LOCUS       DAZKMK010000001       115905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000001 DAZKMK010000000
VERSION     DAZKMK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 115905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.001"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(606..962)
                     /locus_tag="ACG8V1_000001"
     CDS             complement(606..962)
                     /locus_tag="ACG8V1_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0328135.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="ACG8V1_000002"
     CDS             complement(976..1386)
                     /locus_tag="ACG8V1_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0328136.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="ACG8V1_000003"
     CDS             complement(1732..3918)
                     /locus_tag="ACG8V1_000003"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0328137.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="ACG8V1_000004"
     CDS             4136..4819
                     /locus_tag="ACG8V1_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0328138.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="ACG8V1_000005"
     CDS             complement(4900..5619)
                     /locus_tag="ACG8V1_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0328139.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="ACG8V1_000006"
     CDS             complement(5931..6137)
                     /locus_tag="ACG8V1_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328140.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="ACG8V1_000007"
     CDS             6382..6984
                     /locus_tag="ACG8V1_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0328141.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8405
                     /locus_tag="ACG8V1_000008"
     CDS             7125..8405
                     /locus_tag="ACG8V1_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0328142.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8418..9146
                     /locus_tag="ACG8V1_000009"
     CDS             8418..9146
                     /locus_tag="ACG8V1_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0328143.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9215..10738)
                     /locus_tag="ACG8V1_000010"
     CDS             complement(9215..10738)
                     /locus_tag="ACG8V1_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0328144.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10990..12171
                     /locus_tag="ACG8V1_000011"
     CDS             10990..12171
                     /locus_tag="ACG8V1_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0328145.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12231..13859)
                     /gene="treC"
                     /locus_tag="ACG8V1_000012"
     CDS             complement(12231..13859)
                     /gene="treC"
                     /locus_tag="ACG8V1_000012"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0328146.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14310..17021)
                     /locus_tag="ACG8V1_000013"
     CDS             complement(14310..17021)
                     /locus_tag="ACG8V1_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0328147.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17031..17732)
                     /locus_tag="ACG8V1_000014"
     CDS             complement(17031..17732)
                     /locus_tag="ACG8V1_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328148.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17918..18517
                     /locus_tag="ACG8V1_000015"
     CDS             17918..18517
                     /locus_tag="ACG8V1_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0328149.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18598..19611
                     /locus_tag="ACG8V1_000016"
     CDS             18598..19611
                     /locus_tag="ACG8V1_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0328150.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19719..20636
                     /gene="rbsK"
                     /locus_tag="ACG8V1_000017"
     CDS             19719..20636
                     /gene="rbsK"
                     /locus_tag="ACG8V1_000017"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0328151.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20664..21059
                     /gene="rbsD"
                     /locus_tag="ACG8V1_000018"
     CDS             20664..21059
                     /gene="rbsD"
                     /locus_tag="ACG8V1_000018"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0328152.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21059..22432
                     /locus_tag="ACG8V1_000019"
     CDS             21059..22432
                     /locus_tag="ACG8V1_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0328153.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22789..24933
                     /locus_tag="ACG8V1_000020"
     CDS             22789..24933
                     /locus_tag="ACG8V1_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0328154.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24985..26025)
                     /locus_tag="ACG8V1_000021"
     CDS             complement(24985..26025)
                     /locus_tag="ACG8V1_000021"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0328155.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26302..28104)
                     /locus_tag="ACG8V1_000022"
     CDS             complement(26302..28104)
                     /locus_tag="ACG8V1_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328156.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28118..28870)
                     /locus_tag="ACG8V1_000023"
     CDS             complement(28118..28870)
                     /locus_tag="ACG8V1_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328157.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28879..29241)
                     /locus_tag="ACG8V1_000024"
     CDS             complement(28879..29241)
                     /locus_tag="ACG8V1_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0328158.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29417..30289)
                     /locus_tag="ACG8V1_000025"
     CDS             complement(29417..30289)
                     /locus_tag="ACG8V1_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0328159.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30340..30828)
                     /locus_tag="ACG8V1_000026"
     CDS             complement(30340..30828)
                     /locus_tag="ACG8V1_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0328160.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30966..31757)
                     /locus_tag="ACG8V1_000027"
     CDS             complement(30966..31757)
                     /locus_tag="ACG8V1_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0328161.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31812..32420)
                     /locus_tag="ACG8V1_000028"
     CDS             complement(31812..32420)
                     /locus_tag="ACG8V1_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0328162.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32413..32730)
                     /locus_tag="ACG8V1_000029"
     CDS             complement(32413..32730)
                     /locus_tag="ACG8V1_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0328163.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32886..33614)
                     /locus_tag="ACG8V1_000030"
     CDS             complement(32886..33614)
                     /locus_tag="ACG8V1_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0328164.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33773..34654
                     /locus_tag="ACG8V1_000031"
     CDS             33773..34654
                     /locus_tag="ACG8V1_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0328165.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34891..35934)
                     /locus_tag="ACG8V1_000032"
     CDS             complement(34891..35934)
                     /locus_tag="ACG8V1_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0328166.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35937..36605)
                     /locus_tag="ACG8V1_000033"
     CDS             complement(35937..36605)
                     /locus_tag="ACG8V1_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0328167.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36747..39083
                     /locus_tag="ACG8V1_000034"
     CDS             36747..39083
                     /locus_tag="ACG8V1_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0328168.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39198..39509)
                     /locus_tag="ACG8V1_000035"
     CDS             complement(39198..39509)
                     /locus_tag="ACG8V1_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328169.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39690..41165
                     /locus_tag="ACG8V1_000036"
     CDS             39690..41165
                     /locus_tag="ACG8V1_000036"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0328170.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41212..41796
                     /locus_tag="ACG8V1_000037"
     CDS             41212..41796
                     /locus_tag="ACG8V1_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0328171.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41798..42217
                     /locus_tag="ACG8V1_000038"
     CDS             41798..42217
                     /locus_tag="ACG8V1_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0328172.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42498..42782)
                     /locus_tag="ACG8V1_000039"
     CDS             complement(42498..42782)
                     /locus_tag="ACG8V1_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0328173.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43134..43844)
                     /locus_tag="ACG8V1_000040"
     CDS             complement(43134..43844)
                     /locus_tag="ACG8V1_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0328174.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43841..45313)
                     /locus_tag="ACG8V1_000041"
     CDS             complement(43841..45313)
                     /locus_tag="ACG8V1_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0328175.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45451..45924
                     /locus_tag="ACG8V1_000042"
     CDS             45451..45924
                     /locus_tag="ACG8V1_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0328176.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(46077..46628)
                     /locus_tag="ACG8V1_000043"
     CDS             complement(46077..46628)
                     /locus_tag="ACG8V1_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0328177.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46733..47263
                     /locus_tag="ACG8V1_000044"
     CDS             46733..47263
                     /locus_tag="ACG8V1_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0328178.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47746..48189)
                     /locus_tag="ACG8V1_000045"
     CDS             complement(47746..48189)
                     /locus_tag="ACG8V1_000045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0328179.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48205..49365)
                     /locus_tag="ACG8V1_000046"
     CDS             complement(48205..49365)
                     /locus_tag="ACG8V1_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0328180.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49872..51572
                     /locus_tag="ACG8V1_000047"
     CDS             49872..51572
                     /locus_tag="ACG8V1_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328181.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51674..52507)
                     /locus_tag="ACG8V1_000048"
     CDS             complement(51674..52507)
                     /locus_tag="ACG8V1_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0328182.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52811..54976)
                     /locus_tag="ACG8V1_000049"
     CDS             complement(52811..54976)
                     /locus_tag="ACG8V1_000049"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0328183.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55236..57530
                     /locus_tag="ACG8V1_000050"
     CDS             55236..57530
                     /locus_tag="ACG8V1_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0328184.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57523..58188
                     /gene="pgmB"
                     /locus_tag="ACG8V1_000051"
     CDS             57523..58188
                     /gene="pgmB"
                     /locus_tag="ACG8V1_000051"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0328185.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58181..59188
                     /locus_tag="ACG8V1_000052"
     CDS             58181..59188
                     /locus_tag="ACG8V1_000052"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0328186.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59210..60229
                     /locus_tag="ACG8V1_000053"
     CDS             59210..60229
                     /locus_tag="ACG8V1_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0328187.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="ACG8V1_000054"
     CDS             complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="ACG8V1_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0328188.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61986..63407)
                     /gene="tyrP"
                     /locus_tag="ACG8V1_000055"
     CDS             complement(61986..63407)
                     /gene="tyrP"
                     /locus_tag="ACG8V1_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0328189.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="ACG8V1_000056"
     CDS             complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="ACG8V1_000056"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0328190.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65818..67074)
                     /gene="tyrS"
                     /locus_tag="ACG8V1_000057"
     CDS             complement(65818..67074)
                     /gene="tyrS"
                     /locus_tag="ACG8V1_000057"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0328191.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(67186..67391)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            67654..68955
                     /locus_tag="ACG8V1_000058"
     CDS             67654..68955
                     /locus_tag="ACG8V1_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0328192.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(69024..69623)
                     /locus_tag="ACG8V1_000059"
     CDS             complement(69024..69623)
                     /locus_tag="ACG8V1_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0328193.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            69877..70758
                     /locus_tag="ACG8V1_000060"
     CDS             69877..70758
                     /locus_tag="ACG8V1_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0328194.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            70771..72381
                     /locus_tag="ACG8V1_000061"
     CDS             70771..72381
                     /locus_tag="ACG8V1_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328195.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(72458..73117)
                     /locus_tag="ACG8V1_000062"
     CDS             complement(72458..73117)
                     /locus_tag="ACG8V1_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0328196.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(73400..74065)
                     /locus_tag="ACG8V1_000063"
     CDS             complement(73400..74065)
                     /locus_tag="ACG8V1_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0328197.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(74212..74817)
                     /gene="dhaL"
                     /locus_tag="ACG8V1_000064"
     CDS             complement(74212..74817)
                     /gene="dhaL"
                     /locus_tag="ACG8V1_000064"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0328198.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(74845..75855)
                     /gene="dhaK"
                     /locus_tag="ACG8V1_000065"
     CDS             complement(74845..75855)
                     /gene="dhaK"
                     /locus_tag="ACG8V1_000065"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0328199.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(75830..76225)
                     /gene="dhaM"
                     /locus_tag="ACG8V1_000066"
     CDS             complement(75830..76225)
                     /gene="dhaM"
                     /locus_tag="ACG8V1_000066"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0328200.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            76379..76699
                     /locus_tag="ACG8V1_000067"
     CDS             76379..76699
                     /locus_tag="ACG8V1_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0328201.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(76811..77668)
                     /locus_tag="ACG8V1_000068"
     CDS             complement(76811..77668)
                     /locus_tag="ACG8V1_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0328202.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(77776..79080)
                     /locus_tag="ACG8V1_000069"
     CDS             complement(77776..79080)
                     /locus_tag="ACG8V1_000069"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0328203.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(79077..79586)
                     /locus_tag="ACG8V1_000070"
     CDS             complement(79077..79586)
                     /locus_tag="ACG8V1_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328204.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(79761..81896)
                     /locus_tag="ACG8V1_000071"
     CDS             complement(79761..81896)
                     /locus_tag="ACG8V1_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0328205.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(81979..83634)
                     /locus_tag="ACG8V1_000072"
     CDS             complement(81979..83634)
                     /locus_tag="ACG8V1_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0328206.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(83636..83950)
                     /locus_tag="ACG8V1_000073"
     CDS             complement(83636..83950)
                     /locus_tag="ACG8V1_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0328207.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            84120..84380
                     /locus_tag="ACG8V1_000074"
     CDS             84120..84380
                     /locus_tag="ACG8V1_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0328208.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            84394..84708
                     /locus_tag="ACG8V1_000075"
     CDS             84394..84708
                     /locus_tag="ACG8V1_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0328209.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(85438..85992)
                     /locus_tag="ACG8V1_000076"
     CDS             complement(85438..85992)
                     /locus_tag="ACG8V1_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0328210.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(86118..88047)
                     /locus_tag="ACG8V1_000077"
                     /pseudo
     CDS             complement(86118..88047)
                     /locus_tag="ACG8V1_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(88353..89069)
                     /locus_tag="ACG8V1_000078"
     CDS             complement(88353..89069)
                     /locus_tag="ACG8V1_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0328211.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(89070..90893)
                     /gene="glpO"
                     /locus_tag="ACG8V1_000079"
     CDS             complement(89070..90893)
                     /gene="glpO"
                     /locus_tag="ACG8V1_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0328212.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(90907..92403)
                     /gene="glpK"
                     /locus_tag="ACG8V1_000080"
     CDS             complement(90907..92403)
                     /gene="glpK"
                     /locus_tag="ACG8V1_000080"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0328213.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(92539..93999)
                     /locus_tag="ACG8V1_000081"
     CDS             complement(92539..93999)
                     /locus_tag="ACG8V1_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0328214.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(93989..95284)
                     /locus_tag="ACG8V1_000082"
     CDS             complement(93989..95284)
                     /locus_tag="ACG8V1_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0328215.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(95616..96293)
                     /locus_tag="ACG8V1_000083"
     CDS             complement(95616..96293)
                     /locus_tag="ACG8V1_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0328216.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(96323..97414)
                     /locus_tag="ACG8V1_000084"
     CDS             complement(96323..97414)
                     /locus_tag="ACG8V1_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0328217.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(97515..97949)
                     /locus_tag="ACG8V1_000085"
     CDS             complement(97515..97949)
                     /locus_tag="ACG8V1_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0328218.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(98533..99990)
                     /locus_tag="ACG8V1_000086"
     CDS             complement(98533..99990)
                     /locus_tag="ACG8V1_000086"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0328219.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(99987..101882)
                     /locus_tag="ACG8V1_000087"
     CDS             complement(99987..101882)
                     /locus_tag="ACG8V1_000087"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0328220.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(101940..103715)
                     /locus_tag="ACG8V1_000088"
     CDS             complement(101940..103715)
                     /locus_tag="ACG8V1_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0328221.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(103765..105261)
                     /locus_tag="ACG8V1_000089"
     CDS             complement(103765..105261)
                     /locus_tag="ACG8V1_000089"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0328222.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(105455..107077)
                     /locus_tag="ACG8V1_000090"
     CDS             complement(105455..107077)
                     /locus_tag="ACG8V1_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0328223.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(107405..108718)
                     /locus_tag="ACG8V1_000091"
     CDS             complement(107405..108718)
                     /locus_tag="ACG8V1_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0328224.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(108963..109187)
                     /locus_tag="ACG8V1_000092"
     CDS             complement(108963..109187)
                     /locus_tag="ACG8V1_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328225.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(109207..109629)
                     /locus_tag="ACG8V1_000093"
     CDS             complement(109207..109629)
                     /locus_tag="ACG8V1_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0328226.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(109771..110481)
                     /locus_tag="ACG8V1_000094"
     CDS             complement(109771..110481)
                     /locus_tag="ACG8V1_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0328227.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(110499..110996)
                     /locus_tag="ACG8V1_000095"
     CDS             complement(110499..110996)
                     /locus_tag="ACG8V1_000095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0328228.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(111033..111485)
                     /locus_tag="ACG8V1_000096"
     CDS             complement(111033..111485)
                     /locus_tag="ACG8V1_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0328229.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(111620..112225)
                     /locus_tag="ACG8V1_000097"
     CDS             complement(111620..112225)
                     /locus_tag="ACG8V1_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0328230.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(112675..113295)
                     /locus_tag="ACG8V1_000098"
     CDS             complement(112675..113295)
                     /locus_tag="ACG8V1_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0328231.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(113297..114166)
                     /locus_tag="ACG8V1_000099"
     CDS             complement(113297..114166)
                     /locus_tag="ACG8V1_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0328232.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(114189..114596)
                     /locus_tag="ACG8V1_000100"
     CDS             complement(114189..114596)
                     /locus_tag="ACG8V1_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0328233.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(114615..115904)
                     /locus_tag="ACG8V1_000101"
     CDS             complement(114615..115904)
                     /locus_tag="ACG8V1_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0328234.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        33904 a        23946 c        20250 g        37805 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7381 ttttaggaga tcacttaaca agcatcggcg atttttttgt tttgaaatct tcggttattg
     7441 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7501 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7561 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7621 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7681 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7741 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7801 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7861 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7921 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7981 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     8041 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     8101 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     8161 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     8221 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     8281 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8341 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8401 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8461 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8521 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8581 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8641 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8701 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8761 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8821 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8881 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8941 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     9001 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     9061 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     9121 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     9181 agccgatcca aacccttcgg ttcttttttg cctatcagtg caaaagcatt tcctgtgcaa
     9241 tttcacgtcc atccattgta tctggataaa atgttggcca attctccatt tctttcagca
     9301 atacttcttg actttcattt cctgcatata aatgaaaatg ttcggagtca gccggtgcaa
     9361 tcgtgtgatc actgaattga atatatctaa aagcgccact ttccttatct tctgcttcga
     9421 atagatacct tacgccacgg tttcctgcct cgtaggtcaa gatttctttc cctacataac
     9481 gataatttga acgtttcgtt gttccattct taaacgtaaa tgccatctct ttctcactga
     9541 tcttgattga agcaatatct gtttgatagc ccttcgtata atattcttta tattcttgtg
     9601 ctgtcttatc ctgattcaat ttttctttat attcaaaaac aggatcaaaa gtcccatctt
     9661 ctacatacgt atctactgtt tgccaagttc ctgcccaatc tgaaagagag cgatcttgta
     9721 catcctcgtc ttcaaaataa ccgttgtaga cgctcttttc ttttttgggg gtcaaagtat
     9781 cttctagcgg gttttctgtc tctgtcgttt ttttcaacgc ttcgaggttt gcttccatga
     9841 tagaaagata gttttctcca ttttctattt gttcattcgt caatccttcc aaaggattca
     9901 atacttctaa agatacacct gcttcgtttg ctaacgttct ggcaatacta tctttggcgt
     9961 tttcttcaaa ataaatatag ttgattccat gttctttaac aaagtgtttc agttcagcaa
    10021 ttctcgccgc agacggttct tcactagaag aaagaccagc aatcgatact tgattcaatc
    10081 catattctaa cgctagatag ttgaatgcag catgttgcgt gacaaaagat ctttgttttg
    10141 cttgcttaaa ggtttcttga taaagctgat ggagtgcttg aagtttctcg gtataggctg
    10201 ctgcattttt tgtccatact tcctgttttt ctgggtaagc ttcaatcagc tgatcacgta
    10261 tgtttgctac ctgcttgatt gctaaacttg gagctagcca tacatgtggg tctagctcat
    10321 gtgtatggcc ttcttccccg tgttcatggt catgatcatg tccttcttca cttcccggaa
    10381 gtaaaagcat accttctgtc gcttttatcg tatggacatt gccttcttgt aatgtttttt
    10441 ggattgcggg gacccatgtt tccatgtttt catcattata aacaaagata tctgtatctt
    10501 ggatctttgc catgtcttta gcagaaggtt cataatcgtg ggaatcagtc cctgctggaa
    10561 tcaataactc tacttctcct tcatccccga cgattgcttt tgtaaaatca tacatgggat
    10621 aaaaggtcgt catcacgctc aatttttctt tactgtcctc ggctttccca cttgccccgc
    10681 aagcagttaa catacctgct aaaaccagca tggccaatat acctatcaat cgtttcattt
    10741 atgacactcc tcttctcatc ttctttcatt tgaatgttcc gattcgcttt tcgcaaaccg
    10801 taatagttac gttttagcaa caggatttaa tttatcatat ggttaaaaga actgtcaagc
    10861 gttttcttat tttaattgat gagaaagttt atatgaatga ttgacgatat ttttgcaatc
    10921 aacctctatc attttccttt tatacccgct tttgtctata atgaatttat agataaagga
    10981 ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt tgatccgctt tttaggagga
    11041 aaaacctctt actatgtttt aggaatagtt atcttacttg cgctgatcat ttattttttc
    11101 catcagatct cgtttatatt caagcctgtc ggaatcatta tcgctactat cttgccccca
    11161 ttattgtttg cactgatctt gtattatctt ttggaaccag tcatcaaacg tttgagcaag
    11221 cacctttcga ggaacttgtc agtcattatc gtttatgtga ttattttatt tttattgggg
    11281 cttggaagca tatggcttat tccttttctt gaagaacagg caaagacatt gatcagtagt
    11341 ttgccagatt tgtttcaaga ttttcaagag agtctccgac aatttttgga gaaaacacct
    11401 tttgccgatt catttaatca atttttcgct tctattgacg acgtgacgtc tgatatagct
    11461 ggatttattg gaaattattg ggaaagtggc gcacaacgga tcagcaacat tttttcaact
    11521 gttacagcta tttttattac tttatttact ggtccaattg tcgctttttt ccttttgaga
    11581 gatcctcaaa aattttatca gtcagtttta gcgattgtcc caccagcatt tcgtacagat
    11641 tttaaaaatt tgacaaagat tgctaatcaa caattgggtt catttctaaa ggggcaaatc
    11701 attgcttcat ttatccttgg agcagtctac tgggtttgtt tcttactgat cggattagaa
    11761 tttgctagtg ttttagctat ttctgctggg ttgttatgta tcattccata tattgggccg
    11821 tttatcgcct tctttccagg tttgatcatt gcattccaag actctacttt tatggctgtc
    11881 aaatttgtca ttgtctggtt tgccgtacaa cttctccacg gtgacttggt gattcctcga
    11941 gtaatgggtg atcgtttaca gatccatccg attacgatct taatcgtttt acttgtcatg
    12001 ggtgatctaa tgggaattgt gggcgttatt tttggtattc ctatctatac gttagtcaaa
    12061 ttactcgtta cttttatttt ccgtaaattc aaacaacgct acaataaatt ttatggcgat
    12121 aaaggagagt atgaaaactc ttcattttct gaagaagatt attttaaata aaacacaaaa
    12181 cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg tgtttttgtt ttaatttttc
    12241 agcaaagcaa ttgcctggta tggttttagc tccagacttt gatgaagaac agtatcggaa
    12301 taattatcga taagaatatt ggcttctgtg aatccttctg ggatggcgat ccttgtatct
    12361 ttatcataga aatttgttaa tacaagcagc tgctgtcctt caaattggcg aagaaagcca
    12421 taaacttccg gatgatttgc ttcataagca tgataatctc cttgtgcaac gattggatat
    12481 gtttttctca gccgaatcaa tttcttgtaa taggacaaaa tagaatcttc agaagatagt
    12541 tctttttcta cattaatctc ttctagcttg cctccaactt ttaaccaagg tttccctgta
    12601 gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg ttcgagagtt atcgcgagat
    12661 tttgctttga tccgacgaaa cgcttctttt tctgtgaatc cttgttcaag gagtaactta
    12721 taagcattca aactttctac gtcaatatag tcggaaatcg atgaatagtc tgggtcgatc
    12781 atgccgattt cttctcccat ataaatatag ggcgtcccac ggttcaaatg gatcatggtt
    12841 gctagcatct ctgctccttg ttttcgatac tgatcgatag ctacaaatcg attgagcgca
    12901 cgtggctgat catgattatt aaaaaataga gcattccagc cgttaccctc actcatcttt
    12961 tctccccatg tatgaaatag tcgctttaat tctgcaaaat caaatggagg agtagaccat
    13021 ttattcccat tttcataatc gactttcaaa tgatggaaat taaagaccat tgataattct
    13081 tctctactag gttctgtata caagatacaa ttttcgatcg tcgtagagct catttcaccg
    13141 actgtgatga tagtggaatc cgtcccaaac gttgcttggt tcaattcttt tagatacgta
    13201 tgggtgattg gtttgtccgt atattcaggt ttgccatcat tttctttatt atttttcaat
    13261 tgttcgtctt taccgataac attgataaca tcgaaacgga atccttttac ccctttttcc
    13321 atccaaaaac gaacaacttt gaatagttcc tctcttactg cttggttccg ccagttcaag
    13381 tcagcttgtg tccgatcata taagtgcaaa taatatttcc ccgtatctcc aaatggcgac
    13441 caagcacttc caccaaattt tgatacccaa tccgttggct cttctcttaa aatgaagaag
    13501 tcctgataat gctggtcccc tgctagtgct tttttgaacc attcatgttc tgtcgaaacg
    13561 tgattcagca ccatatcgag catgatttct atccggtatt tctttgcttc ggctattagc
    13621 tgttcgaatt cctccatcgt accaaagata ggatcgatgg ctgtgtaatc agagatatca
    13681 taaccgttgt ctttttgagg gcttggataa atagggttta gccaaatcat gtcgatgcct
    13741 agatcattca gatacgggag tttttctttg attcctttta aatctccaat tccatcacca
    13801 ttactgtctt tgtaagattt gggatagatt tggtaaatga ctttgtcttt aaatgtcatg
    13861 gatatttacc tccattcaac tgatttatta cctaccacaa cttatatata caagttatca
    13921 tttattatta taactgaacc gaaagcgttt gtaaaattta tctttataca ttctaagagg
    13981 tctgttgccc aataaaaaac aagagatcag acatgtattt gtctatcctc ttgtttaaaa
    14041 tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa agcttttatc tacggcaatc
    14101 agtcttctca aatgtaaaac atgaggagct aattttattt gagtattctt tttgctcgat
    14161 gcaggacggc ttttttacaa cctctgattc atggtgttca atcagctgtc cttcggtatt
    14221 agctcaaaaa acggcaaaat atgaaaaact attttgccgt ttgtttctct aatactcagg
    14281 tcaaaccgct gatttcacaa cctctgtttt tattcattcg atttcaacgc atcaatcata
    14341 tcgatatgct ttaatttgaa atgggtaaca atcatcacaa taaccgtaaa tacgatagtt
    14401 aatcctgctg ataagacata agcccaccat gtgatcacta atggaaagat gacattctcc
    14461 atagacgctt gttgcaagat gaagtcagtc aatatatatc ctacaccaaa tcctccgatg
    14521 attcctaaaa gagtaaagat gatattttct cgaacaatgt acatcgtgac ttctttatca
    14581 aaaaatccaa ggacttttat tgtagataat tcgcgtacac gctcggaaat attgatatta
    14641 gttagattat acaagacgat aaaagccagc agtcctgata atacgacaaa gattaagaca
    14701 accgagtcta agttcgccat tgattcttct tgcgtttcga tttgtttgga catgaaagac
    14761 gtatttgtca cagcgtctgt ggcaagcagt ttctcggaaa gcatttcttc ttcttttgtt
    14821 gtcatcttat ctgtacgcag caaaaatgca ttatccatca tcgattgatc agtaactgaa
    14881 gcataatatt caggggacat ataaataaag ctacctagat agtttttcac gatttctttt
    14941 acttcgactt tccattcttt ttgatcttca tcataaagcg aaatatgatc tcctgtagat
    15001 aagtcaaata gttctgctac tttttgcgta atagcgattc ctgttttagg caaggagcgt
    15061 gggttctctt caagatcagt taaatggata aaacgatcca aatcagctgt tttacttggt
    15121 acgatcactg tcagactttg cgaagcatgt cctttttttc tcaattctac ggtttgagta
    15181 tgaatagcta ggatctcatt tactttttct tgcttctgca gcatatccgc tgctttttga
    15241 cgactatctt ccgtggatga atccaatgta acgattgctt gatagtcaat gatcggtcca
    15301 aattgtttgg ctgaaacaga acttagagaa tctttcaatc ccacgcctgc aaccatcagc
    15361 ccggcacatc cagcaattcc gataatcgcc ataagcattc ttgacttata tcggaagaga
    15421 tttcggtaac tgattttttg attaaagcta aggcgtgacc acagcggagt gatgtattca
    15481 agaaaaatgc gtttccctgg cttcggtgct ttcggttgaa gcaatgcagc aggccgttct
    15541 cttaaatctt tgaataaaac gatcattgca gcgccaaatg cggcaatgaa gaatgcggca
    15601 gctgcttgta aaatcggcgg ccagtcataa aaaataagcg cactcccaat atcataccgc
    15661 tcgttagaca gctcaaaaat gatccttggc aaaagattcg ttcctaatat cgttcctaaa
    15721 acaataccta tccctgaggc tagacttgca tagattgcat atttttttgc gatttcaact
    15781 ttcgtataac ctaacgcctt taatgtcccg atttccctgc ggttttcttc taccatacgt
    15841 gtcattgtcg taaaggtgat caatgcggca ataaagaaaa agaaaacagg aaaaacattg
    15901 gcaatcgcag cgatacgatc agacagatca ccaaattctt gaaaaccgac attttctgaa
    15961 cgttcattgt ataggtatga tggtttagtt aaattttcaa ttttttctgc atttcgattc
    16021 aattcttcct ctgcagaggc cagttcacgt tctttctcaa ctaattgcct ttcttgttca
    16081 gcaagttgag cttcttgttg agataatgtt tgaacagcga ttgttcgttg cgcttcagga
    16141 agctgctgga tagttgctga ctgttgcgct aactgttttt tagcatcttc taaccgtgtt
    16201 cttgcttgcg ttgtttgtgc tttgccatcc gctatttttt gctgtgccgg ttctaattct
    16261 tcgttggccg attttttcag ctcttctaag cgttcttcgg gtcgatcttg taaatacttt
    16321 tctgcctttt cttgattttg ctccatttta tctttgtaag ctttgctgta cgtattcaaa
    16381 ttctttacgt ttgtaaaatc aagatataag acagaataaa tatctgtttt aaaaacggat
    16441 tcaggtaaat agacaaaata atcgacgcta ccatttccga cgttcgcgta tcctctctcc
    16501 aaattgttga tgaacaatgg agaacggaca aagcccgtga tggtatactc tttttctttc
    16561 aattcatctg aatcaatctg ataaatatca ccgattttat acccttcatc ttccgctttt
    16621 tcatccaata caagctggtt tggtttttcc gggagttttc catcagttaa ttgtagcttg
    16681 ttctgcttgt tatttttgtt ataagaaaat atttgaatca cttcgttttt gccagtattg
    16741 gaataataga tctgttttcc tgtttctact tttgcatctg ggatcttttc tgcttgagca
    16801 atatcttttt gtgtcaagcc accagtagaa acgatttgca aatcagaaag tcctgctgta
    16861 ccaacatagt gatccatcgt tttttgcatg acaggtccag cagttttgac acctacaaat
    16921 aaaagtgttc ctagcatgac aataaggatg attgcaatga atcgtccttt agattgccga
    16981 atttcgcgaa aacctgcttt aagatatgtt cttgttttca taaaatcccc ctaccataca
    17041 atctcatcaa tggatgaagg atgatcattt aattcaacag accttacttt agcatcatta
    17101 atgtgaatga ctctatcagc gatgggcgct aaagcagaat tatgtgtaat gatcaaaacg
    17161 gtattgccgt gttgtctgct agcgttttgc aataatttca atacttgctt gcccgtttca
    17221 aaatccagcg cgcctgtcgg ttcatcacat aaaagaagtt tgggattttt agctagtgca
    17281 cgagcaattg aaaccctctg ctgttcgcct cctgataatt gggacggaaa gttattcaat
    17341 cgatgagcta ggcccacttg cctcaatacc tctacaggat caagcgcatc aggagaaact
    17401 tccgttgcca attccacatt ttcttttgct gttagattag gaacgagatt gtagaattgg
    17461 aaaacgaacc ctacatctgt tcgtcggtaa gcagtaagct gtttatctga aaattgcgca
    17521 atatctgtgt catcaataat gatctgacct tcatcaggtg tatccatccc acctaaaata
    17581 ttaagaatcg ttgactttcc tgctccactt ggaccaagta taatcactaa ttcgcctttt
    17641 tcaattgaaa aagaaatatc gtcatttgct gtaatggtcg tttctcccat tttgtatcgt
    17701 ttgtattcat tttttacttc gacatacttc actctgattc ctccattttc acgatatttc
    17761 tcgctatatc tatgcatcga atgcgttttc tcacacttat ttattttacc actgaatttc
    17821 tctagactaa agaaaggaat cctttaattt agttgacaaa taaacgattt atgatttact
    17881 tacataaaaa acattgttat ttgaaagaag gaacatcatg aatgatttaa aggaaaatat
    17941 gatttttgat ttatcaaaaa ctgcttttgt cgcgatcgac atgcaaaaag agatcgtaaa
    18001 taacggtaca ctctctccac acactgcagc atctattctg acaaacaatg atttgttagt
    18061 aagaacattg aaacgtaccg ctgccttgaa agttttagtg aacgtagaca tctccacttt
    18121 cccttatttg agtcaacaaa ctgatatggg tggaatgact gcttctgtac ctccagaatt
    18181 cactgattta cttttgaagg attctttgaa agatacagat aatatgttga ctatcacaaa
    18241 atacaatcca agtgcttttt ttggtacttc tcttgatctt caacttcgcc ggcgtggaat
    18301 cgaaactatc attttaagcg gtgtagcgac aacaaacggt gtatatgcta ctgcattaga
    18361 tgcctttcaa cacgggtacc atatcgtgct tgctgaagat gcttgcagtg atcgtgacaa
    18421 agaatcccat caattgttca taaaaaagat atttccgaaa actgcacgag tacgctcaac
    18481 gaaacaaata atcgaagcta tccaacaaag caaataagta ctggcacacg tgattttcat
    18541 ttacttcttt ttgatttcat gggaaaataa ccacaggatt tagataggag tatgaacatg
    18601 gtgaaaaaaa agatcacaat caaagaagtc gctaaacttt ccggcgtttc aatcacaact
    18661 gtctccttga tattaaatgg aaatacggag aaattcagtc cgaaaactgt tcagaaagtt
    18721 ttagctgcca aagaagaatt aggctatcag cctgactatt ttgctagaca aatgattacc
    18781 aaggaaacga aaacaattgg tgtactcgtt cctgatatca cgaatccatt cttcagtaca
    18841 ctgatgcgag gaattgagga tatcttgtat aagcagaatt tcgtcacgat attatgtaat
    18901 gctgattctg atcatcaaaa agaaatcgaa tatctagccg agctcacacg tcgaggtgtg
    18961 gatggattca ttatcgcgac ttctgctgtc tctacagatg caatcaacga aaatttgaaa
    19021 aaacaaggcc ggcccttcat tgttttggat cagaaaaaat ctgaaggatt tagtgatgcc
    19081 gtacgaacag acgattttcg cggtggttac ctagcgggca tgcacttgct tagtctagga
    19141 catcaaacga tcgcccttgt ctatcctgaa aatccaccgg aaaatgtcca cgctcggatt
    19201 gaggggttta aatctgctct tgatgtttac caaatccctc acgaccaact gatcctctta
    19261 cctacacagt tctcaaaaca aggtggctac cagatcacag cagaacttct tgattctgct
    19321 gcaactggtg tgtttgcttt aaatgatgaa ttagcttttg gattatatcg cggattagag
    19381 gaagctggaa aaagcatccc tgaagactac agcattattg gctatgataa tatagatatg
    19441 tgcgagtata tcaagccgaa actgacaacg atcgcacaac caatcttcga attgggacaa
    19501 acatctgcca agttattgtt agatcggatc caattcccag aaaaagaatg ggaagaaaaa
    19561 agattacctg tacgattaga aaaaagattt tcaactgcac ccttgaaata gctttcaagg
    19621 tgtgctatat ttttatcgaa ctattaaaac gttttactaa aacgttttaa aaaataattt
    19681 tcccatcaaa ataaatgaga tagttggagg tttgtaaaat gaacacggtt acagtaattg
    19741 gaagtattaa tatggacact acccttcgtt taaccaacat gcccaagcca ggcgaaacga
    19801 tgcatgcgca tgagattttt cacgctggcg gaggtaaagg tgccaaccaa gcagtggcag
    19861 ccaaacgttc aggcgcacgg acatctttta ttggaggcgt tggggctgac agcgaaggac
    19921 aacagttatt agatctactg acaaaagaaa acatcgatac atctgggata gctgaaatcc
    19981 aaggcgccac aaccggacaa gcaatgatta tggtagacgc agcaggagaa aatagtatct
    20041 tgatccatgc aggggcaaat aatgctttcc atgaacaaga agtcctaaaa aacaagcaac
    20101 tgattacaaa cagtgacttt attattgctc agtttgaaag tagcttagat gcgacgatcc
    20161 tagctttttc tattgcaaaa gacgcaggaa aaacaacgat cctgaatcct gcaccagctc
    20221 gtgaaacgat tcctactgaa ctacttgaaa agacagatat cattatacca aatgaaacag
    20281 aaacagaaat cattacagga atcagagtga cagatcataa cagtttggtc gcagcagccg
    20341 aaaaactcca tgaattaggt attggaacag taatcattac actaggcagt gccggcgctt
    20401 tctatcatac agaaaaagaa cacggtatcg tccctgcttt taaagtggat gccgttgaca
    20461 caacagccgc aggtgataca tttattggcg cattaagcag cacacttcag ccagacctta
    20521 gtaacttgaa agaagcgatc ctttatggaa atctagcctc ttctgttgct gtgcagtctt
    20581 atggtgcgca accttctatc ccttaccgcg aagctttaga acaatcagca gtctaaaatt
    20641 aacaatgaga ggaagaaaca aacatgaaaa aaggaaaagt aatcaattca gatatttcac
    20701 gagtaatcgc acaaatgggg cactttgata aattaagtat cggggatgca ggcatgcctg
    20761 tcccctttgg tactgaaaaa atagatttgg ctgtctcaaa cggtcttcca agttttttat
    20821 ctgttttaga aaacgtttta gaagaacttg aggtacaatc gattgaattg gcggaagaaa
    20881 taaagacaat gaacccggaa atattggaaa aaatcaaatt attgttacca gatacaccga
    20941 ttagctttat cccacatgaa gaaatgaaaa aagagttgaa taactgtcat gcatttattc
    21001 gtacaggcga aatgacacct tacgccaata ttatattagt gagtggagtg gtcttctaat
    21061 ggaaacgaca atgaatcctg aacagatggt actgtccgag cagattcata tgctgattga
    21121 acagttggga ggaaaagaca atattgattc tgtccttcca tgtgcgactc ggcttcgtat
    21181 caaattgaaa aaacagccag tagccaatgc agaaaaatta aaacagcaaa tcccttccat
    21241 taagtatttc attttactcg atagatatgc agaaataatc atggatcagg gattggctcc
    21301 tgcttacaag gaattgacgc gtcaaatcga tcattcagct ccgaaagaaa agctaaatcc
    21361 gatcgaaaaa ctatttggtt tgatatcgga aatctttacg ccgatcattc cgctgtttgc
    21421 cggttctggt atattaaaag gtctggttgt tttagcgact acgattggct ggcttagtga
    21481 aacaagcgga acttatcata ttttgtctgc tgcgtcgaat gccgtctttt attttctgcc
    21541 aatctttctt gctttttctg ctgccaaaac gtttaaagtg aatggatata ttgcggctgt
    21601 cattgctgca gcactgattg aaccgaatct gacctcttta ctttcagaca gtggcaaagc
    21661 agctactgat tttctgggga ttcctgtagt actgatgcag tatacgtcta ctgtcatgcc
    21721 ggcgatttta ggtattttct tttattcttt tgttgaaaaa ttcctcaaaa aatatattcg
    21781 cgaaaatatg caattagtat ttgtaccttt actttcatta gttatcacgg ttcctttgac
    21841 gttaatggta ttcgggcctg cgggtgttta ccttggcgaa ggtttagctg ccgcaattac
    21901 ttggatgatg gatattaacg gaccgatcag tggcgcaatc atcgctggtg gttggaatat
    21961 tttggtaata tttggtatcc aatgggctgt aaaccctgtc atgatcagta atatctctgc
    22021 ttatgggttc gaccgaatcg ttccgttgac aggagcagcc aattttggaa tggctggtgc
    22081 agctttaggt gtatttctgc gaagtaaacg ttcaaaaacc cgttcgatct ccggttctgc
    22141 ttttgcttcg attttacttg cgggagtgac cgaaccaact gtttacggaa ttgcgatccc
    22201 attgaaaaaa ccatttgttg ctgcttgcat tggtgcagct gctggtggag ctgtaatggg
    22261 atttgcccaa gtgaaagcga tcgcttttgt atttggtagt ctgaccacat tgcctgcatt
    22321 catttccggt acttttttct ggtacttagc aggactggct gttagtctag tcgtagcaat
    22381 gatcactact ctcgttagcg ggtttgatga agacctaatg tcttatgaat agttaatgaa
    22441 aaagactgaa gtatgagatg tttatcacat atttcagtct ttttcttttt aggatcatta
    22501 tttttataaa tttttgtgat actttccaaa ctttggtata atttacatat gcataaccat
    22561 ttttatcccc acttgtttat ccccaaacaa aggtggtcta tgcaataaaa tgaacgtcaa
    22621 agaaaaacga atgaatgctc gttgcttatt tacaaattgt tttatttaga atacacagac
    22681 agatttatga aaagaaaaag cttttcgaca cgattatctg ttccaaaaaa tgtctaatga
    22741 atgctgaata agacagaaaa tcaatgaaag gaaaaggact agtttattat gaaaaataaa
    22801 aaaacgatac gtttgctagc tgctacttgg ctgattgctc cgtctgtttt gtcgaccaat
    22861 accgtatttg ccgtagataa tcaaatagct gatcaaacag tcacaacaga tacgactaca
    22921 tccagctcag ctactagtga aacaacggat tcttttacag aagacccaac tggaacaact
    22981 aactcaacaa caggatcgac cgagccaact gaaattgatc cggctgatcc atcagaaaat
    23041 gaaaagccag ccgagacata ttcccttaca attgctcctt ctttgattgg taagatccaa
    23101 ctgactgctt cgacagcgga ttcccctgaa cagcaacctt tggatattac ggaaagtgga
    23161 acggtagaag gtttattagc ggaaacgaag gtaacttaca ctattacgcc agctgaaggt
    23221 gaacaaattg cttcagtcgc tgttaatggt gccaaagaag taaattatga tgcaaagacc
    23281 tttacagtag gtacgaacaa tttatctatt actgcagaat ttaaagctgg accggatact
    23341 tcaacagaga aaccaactga tcctagtgaa gaagtcccta gtacaccaga tccggaaacg
    23401 ccaaacaaac cagatgtggg taataacaac aataataatg gttcaaataa ttcaaacaat
    23461 aataacaata attcgaacag cgggaattct tcgaatcaaa caaacccatc tggatcgaat
    23521 aacaatggaa atactcagag agaaccaaac catccaaaca atggttcatc gaatggcgga
    23581 acgaacaatg cttcatctcg tccaaataca gcaaatcaaa gcggtataga gaatccatct
    23641 tctgcttctt ctgattttgt tgtaaaatca ccaattgatg ctgtattgcc tacaaataca
    23701 acgaatgtcc aacaagcaat cgttcgagaa gcattcaagc atcttggaaa accatatgta
    23761 tggggagcaa aaggtccaaa tacttttgac tgttctggtt tgacttatta cgtatatatg
    23821 aaagcaaccg gtcattatat cggtggctgg acaggcgaac agcaatatgc aggtacgcag
    23881 attccagtaa gtcaggccca acctggcgac ctcgtcttct ggggaccttc ttccggtgtt
    23941 actcaccatg taggtatcta tattgggaac ggacaattta tccatgcgcc tcaaccagga
    24001 gataaagttc gagtaacttc tatttcagat ttcacgcctg attttgctgt ccgagtaaac
    24061 ttagcaggtt tacctgctgc ttcaggttcg ctgacaggcg gctcatctat tttagatgga
    24121 ttagatggct cgttccattt cagtcaaaac caaacaactg atcagttctt gaaaaagatt
    24181 gcagatgatg cacaggaaat cgggcaaaaa gaaggaatct acgcatcagt catgatggct
    24241 caagctatcc ttgaaagtgg atctggaaac agcttattat caagcgaacc aaatcataac
    24301 ctttttggta tcaaaggatc atataaagga agcagcgtga ccttcaatac acttgaacaa
    24361 gatagttccg gacaaagcta ccaaatccgc gcacaattcc gtaaatatcc ttcttacaag
    24421 gaaagtttgg aagactatgc cgacttgatc aaaaatggac tgactggcaa tcctgatttc
    24481 tacaaaccaa cttggaagtc ggaaacaaaa gactacaaag aagcaaccaa atatttggaa
    24541 ggacgttatg ctacagatcg ccaatattca caaaaattga acgcgatcat cgaagcgtac
    24601 gacctgacaa aatatgatga accgaaaaaa gaagcagcaa caactgaaga agcaaacgct
    24661 gaaatcctca atatgtctaa gcgattcgat gtgccaatca aatggacaac agatggcgtg
    24721 ccaatcacag gattagaatt ttcaacgata aaacgcgtca acaacgcttc ttccatcttc
    24781 cgtgtcgctt caatctggga tttgtggaac aattttacgg caagacagat accagaaaca
    24841 actactcgta cagttaaagc tgcaactgtt cctgtactaa ccttgttaag tttagacaaa
    24901 tcattctggc ttgatctgcc tttaaagaaa tagaataaat taaaaagggg atgtgacact
    24961 gtcacactcc ctttttaatt tattctattt ctttatattt attttgtatt ccgttacaaa
    25021 ctcgcgtact tggttaggtt ccagagtgac atctccaaaa ctttcaagtc gctccgctcc
    25081 tggcagttct tgagtctcaa atgtgatacc gccatgattt actaatttat tcccgcgcat
    25141 ttccggtcca tcttctccaa aatttgctgt aaaaatcacg attcctgaag catctgtttt
    25201 tacttggatc gatacttttt catctgggct tgtcaattcc gcagcattta gtaaaccaga
    25261 ttgttttttc aacaaaaatg gatgatcgat gccgcccact atcattgatt ggcgaaaatc
    25321 actggcaaag acctcaccta attttttagg acgttggaag tcaaatgctg tgccacttac
    25381 tgaaactact tctcctgtag gcgttgtatc tttattgagc gtagcaaata atcggctatt
    25441 caaccaaagt atatgttcgt cgattgactg tgctggatca ccggttaaat tgaaatagac
    25501 atgatttgta ggattgaata ctgttttgcg gtcactgatc ccttggaccg tcactcgcca
    25561 tgtatcatcg ttcgtcagcg tataccgtac ttctacatct aaagttccag gaaatccgtt
    25621 ttccatatct gggctagtct gatagaaaat gaccgatgct tcgttttcac cattgatgac
    25681 tgtataatgc cactgcttgg cttcaaagct aggtgcacca ccatgtaaac aatgaccagt
    25741 gtccgggtct gtttttactt cgaacgtttc atcatctaat ttgaatgtgc cattcgtaat
    25801 ccgtccagcc atacgtccga ttgaagcacc gatataaggg tctttttcta gatattcttt
    25861 tgcttcatca aaacctaaga tcaactctcg tttttcatta tcgattattg tttgaaaatg
    25921 aacaacccgt gccccaagat cactgacaga caaagatgtc ccattttgat tcgtgatcgt
    25981 aatcaagtga tagttttcgc caaaattttg ttcattgatt tccatgttcc ttgcaccctc
    26041 ttttctttca tttgcactga ttccagtata catggaaacc gttttcttaa cagcttattc
    26101 cgtgagtaac gagaaaaaat ttactatatt ctaataaaag accgtgaaaa aaaggttgtg
    26161 acaaaaatat tgtcacaacc tctttttccg aacaaacggt gttcaaaagc ttgcatctgc
    26221 ggtaataagc ccaaccaaac gaaaaatatg aggagctatt tttgcttggc tgttcttatt
    26281 acttgatgca ggacagcttt ttcacaatct ctttttcaat acgattccag tatataacca
    26341 tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat atcgtaaacg gaacccaaat
    26401 atctttgtat ggattttgca tgaatagacc aaacatttgt aatccgaata agacatggac
    26461 tacacctaag attcctggtg ctaagaataa cacgcctact tctctacgga tcgattgttt
    26521 aagcaacccg cgtcgtgttc ctatcttttc aagcatttca taacgagcaa tgtcactctt
    26581 gcttcctgac aagatcttga acatcaaaca gctagcaagc atggtcaaga aagctaaacc
    26641 taggaagaat cccataaatt caaaaccaga gaatagacca ttatacactt catacatttc
    26701 atatttctgg ttgctgtact ctactttttg caaagatgga ttgttcattt tattttgagc
    26761 aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct tttacctgaa ctagctgtaa
    26821 agtggattgt ggcaagttca gttgatcaaa ttctgattga ctgactacct tgatttcttt
    26881 ttcttgctgt tccggcaatt catagcttcg aagttcctct tgtacagtca gttcattcat
    26941 ttttttagca ggaacttttt ctttcgtgat cttcggcggt gttccatctt catgttttat
    27001 catgatcaat ggttgagccg caaattcact tgcattatag taaatcgttc ccgcatcttc
    27061 tttttgagta taagaagcag aaagtgttgg tgaaagttct ttgatttttt gctgatcttc
    27121 tgcccgagga ttattcatca ctagatcata tccagaattg ttttctgtca agtctgcaat
    27181 ttcattttta aaacctaatc ctaccgtcaa agcgcccagt gccaaggcaa acagcatagc
    27241 gaccattgag agcatttgtg tatattcacg aatacgaaag tttaactgtg acaatgtgaa
    27301 attgttcaat tttttcaatg aaatctgttc tgatttcttt agtaatgata aaaagaaaat
    27361 gatcacagaa tggaaaatga aataagttcc taatacgatc gtaactaagg ctacccctat
    27421 accaaagact tgataggtcc ccacgtgttt catcatatag taaccgcagc ctagaaaagc
    27481 aattcccagt actacttcaa ggaaaaacaa gaatttatgc tgcttgattc ttgttggtgt
    27541 ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca atcgaaaaag cattgacaac
    27601 agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg atcaacaacc cgtgaatact
    27661 aaaaggtgta aaatgcgaaa tattgagatc taactgatcc actaataatc ggtggacgat
    27721 acttgtcaat cctacaccga cagctgaacc cactaaagta gctgcgatcc ctactaagaa
    27781 tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt gctcccagca tcataaacat
    27841 tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt gcatataaaa tataaacgaa
    27901 tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg ataactgtag ccgcaacgat
    27961 cgtattactt ttcaaaaaag cttcgttgct tgccattgat tcaaacatgt aaaaaatagc
    28021 agatgccata actaatcctg agaataacac caagtagtct cttaaacggc tcttgatccc
    28081 agtaagtgat aatttccaaa gcatagtcaa tctcccccta ctgttccaag tttccaagaa
    28141 tagatagtat ttctcgatag aaggaatcac ggctttgatc tccacgtttc acttcttgat
    28201 ggataccgcc atccttgatg aataaaatac gttggcaata acttgcagaa aacggatcat
    28261 gcgtcacaag aagaatcgat acatgatctt ttgtattcaa ttcgtccatc gtatctaaaa
    28321 gatcccgggc acttttggaa tccagcgcac ctgtaggctc atcccctaat agaatcgttg
    28381 gttgtgtgat caatgcacgt gcggcggcga cccgttgttt ttgtccaccg gaaatctcag
    28441 aaggatagct ttccaaaata tgagagatgg ataaacgttc tgcaattttt tggacacgtt
    28501 gcttgatttc ttttggtctg acgccttgta aagaaagagg aacagcgata ttttcttgtg
    28561 ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa gccaatctca ttcgcacgaa
    28621 aatctgctag ttgatttcct tttaatgtgg tcacatcttt ttgattgatt cggatatgcc
    28681 catctgttgg tttatctaat gtggataaaa tattcaaaag tgtcgactta ccagaaccag
    28741 aagcgcccat gatcccaata aattcgcctt tttctacatc gaagctgatg ccgcttaatg
    28801 cttgcgtctt tttctcattg ttttttccgt atacttttgt tacattctgt acttcgacta
    28861 ttttgttcat ttctttccct accctttaat tagtttttcc agtgctgttt tcggcacgtc
    28921 ttttttttcg acttcttcat acgaagtgac acctttattt ttgttccagg tgatttttaa
    28981 atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg aagtccagtt ctttttcctt
    29041 accctctttc gtatagccag gcaggatata tctatagttg actgtttctt gtccagagtc
    29101 tgattttgaa actgtttttg taccatctgt cgtaatttgt acatagtatt cttctccacc
    29161 cataactacg taatctgcga ttttcacccc tgcaagtacc acaacgatga aaacaagtag
    29221 accgatcaga agttttttca ctttttcccc ttctctctta ctttatatac tgattataga
    29281 aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct tctttcaatt ttgtcatttt
    29341 ctttaaaaca actaaaaaaa gagttcaaga caataatccg tcctaaactc cttcttccta
    29401 tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc tgtattgata acaggttgcg
    29461 ttcctttgtc tgtaatgatt gagacaagta aattattgat caattggact ttccgatcat
    29521 cggtgaaatt gatttcttgt ccatcttcga tttgctctaa ggccatttga gtgattgtga
    29581 cagctccttc aacaatggtt tgtcgagcag acaaaatggc ttttgcttgt tgacgttgga
    29641 gcatggcact agcgatttct gttgcatagg ctaaatggtt caagcgtgtt tcgattactt
    29701 cgacacctgc tactgctaaa cgctcttgca gctctttttg caactcgtca gacactgccg
    29761 tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt atcatacggg tattggctag
    29821 cgatatgccg aatggctgtc tcactttgga tttctacaaa atcttgataa tacgctacat
    29881 cgaataatgc ctttgctgta tcaacgactt taaagactac tactgctgag atttcgatgg
    29941 ggtttccatc caaatcattg acttttaaaa cagaactatt gaaattccga actttcaacg
    30001 aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa gccattttct cgaatcgtac
    30061 ctaaatattg tccaaaaaac aaaattactt ttgcttgatt aggactgact acagttgccg
    30121 aacttaacaa caataacgtg atcaaccaaa gaaaaatact taaaaaaagt gcccaaactt
    30181 tcgcttgcca catacctaaa tagaagaaaa acaatccaac taaagctaat acgaccaatc
    30241 cgacaatccc tgcataacca ttgatataaa atgctctttt ttctctcatg ctacttcctg
    30301 cgctccttta tccttcaata aatatctaat gttacttttt taattttttt ttcgaatacg
    30361 cgccattaca ccttctagtt ctaactcttt gtcttcagtg atgtaccaac cgcttgcctg
    30421 tgtccctccg gctgcctgcg gtaaataatc caattttgtt cgaacccagt gcttttcttc
    30481 tgtaaaatag tcttctacca agacctctag atccatccct gctgaaagag gcaaatcttc
    30541 ccgaacagat ttaaagataa atcgttcctt cttcccatca tacttcaata ttcctgtatc
    30601 gataatcggt aaagaataat aatccagctc tttcttcacc gtcaataaat gtttgaaaac
    30661 ttcatggtac acttgctcta attggcgacc ttctgaattg ttcgggtaat taaccatcac
    30721 tttttctttg tctggatcat agaaggtttg gcttagttga gtgatataag tatgtaatct
    30781 ttccatttct gtttttaact tttctctttg accttcttct accttcatat actctttcac
    30841 cctgttttct ttttagatat tttctttctt attttactat agttatgtgc gttaagcgat
    30901 gaaaaacagc aaagattgga aggatctttg ctgttttttc ttaaaatatc cagttgagta
    30961 caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa tattgatcga tgtgtcttta
    31021 gcagtgatct ttagcttttt ctgtccttgt tctaacaaag tatttgttat tggctggtag
    31081 tctttttcaa tataagattc accagacgtt tggatagtat agccactgtt ttccatctca
    31141 tgcaaagata aattgccacc gtttaggatg acttttcctt ctttgctgat caaactgtct
    31201 tctagatgga agttgctgtt ttcacctgtg acgatcaagt tatccacatt ggcctcatct
    31261 aatcgaagtg agcctaattc atctttcgca gttattgttc ctcctgtaaa ttcattgaac
    31321 gatacccagc catcatgtgt cttagtattc acttttttga ttgatacttc gttgatatca
    31381 attgcactat tattcccttc gattgtcaat tcagataatt catgatctgc agggacaaca
    31441 atagtgattt gtgcacttgt gtcttctaaa ctaatgattg cacctgttga ttttttgact
    31501 gcattcacag tcaacacgcc atttttctcc tttatttctg gcttttctgt cttatcataa
    31561 gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa ctgtttgatt ttctgcatgg
    31621 atgacgattt tgtttgcatc cttgattttt tttgtctcag taaccatatc gatcaaccga
    31681 ggaccattat cccaagtcag actcttttga gcgcctaatg tataagttgc acctgctagg
    31741 aggccaccag taagcatcag accaataccg gtaatcaaaa aatgtttcaa tctcaattta
    31801 tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt ttgtaataaa acgtcgtccc
    31861 atccatttaa caaagttcat catgacataa aaaagccctt ttgttccttt cagcaacagt
    31921 ggcgaaataa atactgcgct tcctgtggcg aacagaccga caccgataaa gaataatcca
    31981 ctcagaatcg attgtccaaa cacactgatt cctgagatga tcatgaatag gccaacacct
    32041 aagatactga tacataaaac atagatacta aaaatgagcg ctagtaaagc aaccaatagt
    32101 cccccaataa ccaatataaa ccccagtgct agtggaatga ggacaggaga agcaaataaa
    32161 ccgataatga ttaaccaaat caggcgaatt tgtctttttg gatttttcac taactcgttt
    32221 tctgcttgtt cttcttctga aaagtaactg atttttaatt ttaaggcaaa ctgctttggt
    32281 gtaccatatt catcaatgat ctgatctaat gtttttccag aatctaaaaa atattcctca
    32341 taatactgga tcaaatcaaa acgatcgtct tcaggaattt cttgaagatg atccacaact
    32401 tcattcaaat attcattcat ttgtgctctc ctccaataac cggtctaaag aatctcgaaa
    32461 aacaatccat tcatcaataa tttcttctaa atgacgactt cccattggtg tcaacgagta
    32521 atatcgacgg tttctgcctt caaaaggttc atcataggtc tcaagatatc cttctttttt
    32581 caatcttctc aaaaccggat atagtgtaga ttcactgaca gagagacttt cttgtacgat
    32641 ttttgttaag gcatacccat acatatcttc cttgcttaat gctgctagca cagtaccatc
    32701 aagaagaaca gttggaatct ggatgtgcat tacgtcacct tctattctca agtgtaatat
    32761 tttatttcaa tctatattat acaacatata atatagtttc aaaatcagtt tgtcaaatac
    32821 aaaaaaactg actaaaagat ttgatccttt ttagtcagca aaaaatggct tctctttgat
    32881 ttttattata acagtggcat aatccaattt gtccacatcc agctcagtgg catgataacg
    32941 atcattgcag gtatcatgtc agctactggg aacattttca cgttgatcat tctaaaaccg
    33001 gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta agatcatgtc tggtgtagtc
    33061 aatggataaa taaattttgc caataagaac aaaatataaa aaataatgaa ttggggaatt
    33121 gctatcacag aaacgacata ccctaaatta cacgcaaaaa tagctgctgt gaaaaaatca
    33181 aggacagatt tagaaatcaa aatcgtactg tctcctgtca tcccagaatc taaacttcca
    33241 tagatccccg taccacttgc acaaaataga acaatgaccg tcactaatgt gctgataaat
    33301 tcatcgtggg tcataccaag cttttcacta gggaagattt tagaaatagg tacttgcatc
    33361 agcgcagccc ccttatttat cccattacct aaatgaacaa tcaaaccaat cgctgttcct
    33421 atcactaaag caaaaataac agccggcata tttttcatcg gcgcaatgga atagattccc
    33481 atccccatcg aacacacacc aaaaaccatc gtcagctgtg ctttaaaatg ttcacttaat
    33541 ttatttccta ctaatccgcc aattagtccg ccaaacaaaa cggaacctac attgatcaaa
    33601 actccagttg gcatcaaaaa actcctctct ttttctacga agcgaaaaaa atcagaaaaa
    33661 tttctgtaat tattgctttc gctgcaagaa agattgtaac agacagagaa gattacacca
    33721 attcattttt tgtgttaaag tattctttat tggaataagt aggaggaata tgatggatat
    33781 ccataaattc gaagtttttc ttgatttagc cgaaacaatg aatttcacca aaactgctga
    33841 tcgacaattc accacccaag gaaatatttc taaacagatt ctttcgttag aaacagaatt
    33901 agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa ttgaccgaag ctggaagctt
    33961 attgctccca tatgtaaaaa atgtcgtaga acaataccat gctatgcaag aagtgcttga
    34021 aacctatacg aaagacaaca atctttcatt aaatatctta acgataccga caatgatcaa
    34081 ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc catcctgaat ttacagtcca
    34141 gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac gccaatgaac atagtgacac
    34201 gatccacttt gctagaagtt tccaggcccc accaagttcg attgagtggt tgcctactga
    34261 aacagacgat tttgtagcag ttctacctaa aaatcatcct ttagctagta aaaagaaatt
    34321 ggatctaagc gaactgaaaa aggagaactt tttgctcctt ggccccacta cgaacttgta
    34381 tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa ccaaaaatct cttataaagg
    34441 agcaagaatc gacttgatca tcaatatgat tgcaaatgac atggggattg ctattgttat
    34501 ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg attctcccta tttctccaac
    34561 aacagagagt tatcttgcat ttactcgtaa agtcggtgag cattctttgg cttcagatac
    34621 attttgggag tatttaaatc aagaagaaaa ctaagcccta cattttctgt ccatctatcc
    34681 acccaaaaaa agagagcgac gcatgacttt cgctctcttt cttgcatcat gtccttgttt
    34741 caatgacacc tctaacattt ctcgtagttc aataagaaag cagcgatttt tatgccaaaa
    34801 gaaccgtaag aacatttccc taatcacaaa aaaacgacta aaaaccccta aaaaccttca
    34861 atagaatttt taggggtttt attctttttc ctattgttta cttaaccgta tcattgctgt
    34921 cgttcctact ccttcgatac tgctataatc aatacgtcca tcatgcagtt ctagaatttt
    34981 tttaaccagt gaaagtccta gcccattccc agaaatttga cgacttgtat ctccttgata
    35041 aaatttttca aacattcgat ctctggtctc ttcattcatt cctacacctg aatcggtcac
    35101 ttccagtaca atctctgtgg ctgtctcaaa tagtttgata tgaatctgtc cattgatttg
    35161 attgtacttt atggcgttat ccatgatatt cagccacact tggtacaaaa actcttcatt
    35221 gcctgtgtaa tttactgcag caagttcgat atccaattct agctgttctt tctcccattt
    35281 aggctgaaaa aaaacgatta cttctctgat ttgttcatct aatcgatact ctttcttttc
    35341 cagttggata cgctggtttt ccagtttgtt caattttaag atgttctctg ttaattgtga
    35401 aagctgcgtg atactttcga taatccgata taaaaatatt tggcgttctt cgtcagagag
    35461 atttggggct tgtaacaatt gaacatatcc ttggattgtt gctagtggtg ttttgaattc
    35521 atgggataca ctggacacaa agtcattcct cagtgtctcg atactattga gttcttggac
    35581 cataacgttg aaatctttat acagttgctg tacttctgct actttctgct gttcatccat
    35641 tcgaatcgaa aaatctcccg ttgctaccag actcatattt gtccctagcg cactgattgg
    35701 atgcaggata cgccgcccaa caaaaatcga tattcctgta cccacgacca tactgaaccc
    35761 cgcaaagacg aataacgggt ataacgttgg ttttccagcc gattcagcag gcgagataga
    35821 atgtcgagac aataagaaaa taaaaccgag aaaacacagc agtgtcacta agatcgaaat
    35881 gaagacaata ctagcaaaat agatccataa ttgtgaaaat agcttttttt tcatctttaa
    35941 ataaccgcct tatagcctag tccccgtact gtgatgattt gaaaatcttc cgtcttttca
    36001 aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc gttcttctgt attcgtatct
    36061 aatccccaaa tatcatccat caactgttgg cgagtgaaga tcttgttggg ataagacagt
    36121 aatttataca gaagcagaaa ttctttttga ggcagaatca acgaatgctg atcttgtaac
    36181 actgtataag catctagctc taattttgtt tgtccgatta tcaagctact gctgtaattg
    36241 atctttgaac gtcgaagcaa tgcttccaca cgtaggatca tctcattgac atcgatgggt
    36301 tttgtcatat aatcatctgc tcctaatttg aacccgcgtt tcttatcctc aaaatcggcc
    36361 tttgccgtaa tcattagaac aggtgtgtca aatctggtgc tacgtaatga gtctagcaag
    36421 tcatagccat ccattttcgg catcatgata tctgtaatga ttaattctat acgtgtcgct
    36481 tctagttttt tcagcgcatc ctctccattc aatgcggtga tgactgaaaa gcccactcgt
    36541 tccaacacag aacggtaaag caactgtaat tgcggatcat cttctactac taaaatacga
    36601 ttcattgatc tccctctgtt ttttgaaaaa tttctagatt ttttcttgta gttcatcttc
    36661 agttaacatt tatttcgtat actacgatca ttgttctgat atacttatta gtataccgaa
    36721 tatttcaata cgggagaagg gtgattatgc ttcaattaaa agatataaaa aaatattata
    36781 aagtcggcga aaccaccacc aaagcattgg atggtgtgtc tgtcgctttc agacaaaaag
    36841 aattcgtagc gattttaggt cctagtggtt ctggtaaaac gactatgctg aatgtgattg
    36901 gtggactaga taattatgat tccggtgata tggtaatcaa cggaaagtct actaaagact
    36961 ttaaggacag tgactgggat gcctaccgaa acaactctat cggtttcatt tttcaaagtt
    37021 acaacttgat tggtcatcta ggcattattg aaaacgttga acttgggatg acattaagtg
    37081 gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc attgcaccga gtcggcttga
    37141 caaatcatat gcataaaaag ccaaatcagt tatctggtgg acaaatgcag cgagtggcca
    37201 tcgctcgagc actagccaat gacccagata tccttttgtg cgatgaacca accggagcgt
    37261 tagatacaga aacaagtatc cagatcatgg aattgatcca agagttatcg aaagaaaaat
    37321 tagtgatcat ggtcacacat aatcctgaac tggcccatca atacgcagat cgaatcattg
    37381 aattttctga tggtaagatc ttaacagatt cccatcctca catcgaacga ccaaaagatg
    37441 atcaatttaa cttacgcaga acaaagatga gtttttggac agcgttaaag ctttccttca
    37501 acaatatccg aacaaaaaaa ggacgtactt tcttgacctc ctttgcttcc agtattggga
    37561 tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt ccaaaaacaa atcgataaca
    37621 cgcaagctga aacgatggcg aaatttccga ttactatttc taaggtaacg actaaccaga
    37681 ctcgtgacga tgccggattg ggtgcaagca aagcggatta tccagacagc aaaacgatta
    37741 ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa taagattgat caaacatacg
    37801 tcgattacgt aacagacatc gatcctaact taagtaataa tatcggattt acccgtacta
    37861 caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca gcctgtcagc ttctcgaatc
    37921 aaaatcctga tacggaatct ctttctttat ctagtacgat gtcagcaatg acaggtgtgg
    37981 gagtgtcaag tttccctaca cagcttgaca ctagcaaaga aaacttttta aaagacaatt
    38041 attctttact tgccggaagt tatccagcct cagcaacaga tgtcgtgctg atcgtagacg
    38101 gcaataataa tactaatatc aacgcattga agaatcttgg atttgatgtg aaagaagacg
    38161 aaaaacttga ttttgatgat atcgttggta caacattcaa attagtaaac aacaatactt
    38221 actatacaaa acttccaact gggaatttta tccctaatac agattatgat gcgatgtatc
    38281 agaatgcttc tgatgaattg aagatctcgg ggatcctacg cgtgaaatct tcttctacaa
    38341 tgaatctctt atctccagga atcgcttata gcgatcaatt aacgactcaa atcgtcaacg
    38401 aaaacaaaga atcagaaatc gtcaaagctc aaagagacag tggtgtaaat gtcttaacta
    38461 ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag ttatttaggt ggagacagct
    38521 tgccttcaag tatcatgatt tatccgaaca attttgaaga caaagaaaag atcttggatt
    38581 atctagatga ttacaataaa ggcaaatcag atgaagataa aatcatttat actgatttag
    38641 ctggaacgat gactgaactg actggcggat tgatggatgc tatcacttat gtccttattg
    38701 cttttgcagg gatctcttta gtaacaagta tgatcatgat cagtatcatt acttatacat
    38761 ctgtgattga acggacaaaa gaaatcggtg tactaaaagc tttaggtgca cgtaaaaaag
    38821 acatcactcg cgtatttgac gcagaaacgt gtattttagg tatttcttct gggatattag
    38881 gtgtgtttat tgcatggcta gctacgttcc ctatcaatag catcttatac aacatgacgg
    38941 atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat tattctagtg atcgtctcta
    39001 ctatattgac gatgcttggc ggtcatcttc ctgcacgaat ggcagcgaaa aaagacgccg
    39061 ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat ttagatttga gtcataagag
    39121 cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat atgttccttg tttcgcgctg
    39181 ttttttttaa ttatttttta taattctggt atacttttct ttcctttgta ccacaactct
    39241 tctggcgtat acctgtcttt tccatttatg aatactgttc catctgcttc aatcgttact
    39301 tcagtcccaa tccacatatt acgagaaagt gcgagaagat tttttctttt tcctcttcct
    39361 ttttttccaa attgcaaggc atcggttatc tcttctgtcc aatgagattt cacgtatttt
    39421 acttctccgt tttcaaatgt aactttcaaa atattcgcta tattttccac ttttactgct
    39481 ttaggaaatg actcctccat ttttttcact ttccttcctc caacactaag tatgcgcttt
    39541 cttttagtga taagtatatc atagtttatt ctattatcaa aaaaacgcat ccattccatt
    39601 gtagaatact ttaccgaaca agttgaattg ttttcctctt tttcaccttt tactatagat
    39661 aggtaaagac aactaaggag gaatctctca tgacagatca accgtatgat ttaagaaaag
    39721 tcatcgaaga actaaaaaca cttccaggtc aatatcacga aacagatatt gaaatcgatc
    39781 ctgaagcaga attatctggt gtttaccgtt atatcggtgc tggcggtaca gtaaaacgtc
    39841 cgactcaaga aggaccagct atgactttca acaatatcaa aggtttcccc ggcacacgtg
    39901 tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga aattttgcac catgattatc
    39961 gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc aatcaaacct gtgaaagtag
    40021 acaaagcaga cgctccagca caagaagtcg ttcatctagc taccgatgca gacttcgata
    40081 ttcgcaacat tttgcctgca cctacaaata ctcctgaaga tgctggtcca tacatcacaa
    40141 tgggtgtagt cttaggatca gatccagaaa aaacaatgag cgatgtaacg atccatcgta
    40201 tggttctaga agataaagat acgatcggta tgtacatcat gcctggcgga cgccacattg
    40261 gtcacttcca aaaacaattc gaagccttga ataaaccaat gcctatcact atcaatattg
    40321 gtttagatcc agcaattgcc attggcacga ctttcgaacc acctaccact ccacttggct
    40381 acaatgaact atggtgtgca ggagctttgc gtaacgaacc tattcaaatc gttgatggta
    40441 tagcagtcga tgaagtgggt attgctcgtg ctgaattcat cattgaagca gaaatcatgc
    40501 caaatgaaac aatgcaagaa gatatcaaca cgaactcagg aaaagcaatg ccagaatttc
    40561 ctggctatac tggggatgct aatccagctg tacaagtagt gaaagtcaaa gcagttactc
    40621 accgaaaaga caatccaatc atgcaaacaa cgattggtcc cagtgaagaa cacgtatcta
    40681 tggccggtat tcctacagaa gcaagtattc ttgccttggt cgataaagct attccaggaa
    40741 aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa attgatgacc atcatgcaaa
    40801 tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca agcagctatt cttgctttat
    40861 cagccttcaa agaattgaag acagtcattt tagtcgatga agatgttgat attttcgaca
    40921 tgaacgatgt agtttggact ttcaacactc gtttccaagg agataaagat atcatcgtga
    40981 tcccaggaat gaggaaccat ccattggacc catcagaatt accagaatat aatcctggct
    41041 ctattcgttt ccgtgggata agtgcaaaaa ctatctttga cggtacagtt cctttcgata
    41101 tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga agattgggaa aaatatttga
    41161 aataatgaat gatctgcaac tgttttgaca tcataaaaag gagtgcggaa aatggaacaa
    41221 ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg gcgcttcagg caccatttat
    41281 gccatcaacc tagtgaagaa actaaaagaa tatccgatga tagaaacaca cggaatcatc
    41341 agtccatggg caaaacagaa cttaaagctg gaatccgact tatcactaac tgaattcgaa
    41401 agcctgtttg attaccatta ttcaaacaaa gatttaggtg ctgctgtcgc cagcggatca
    41461 tttttgaccg atggaatgat catcgtaccg gcaagtatga aaacagtcgc aggtatctct
    41521 gttggtttag gcgacaacct gattgcacgt gctgctgatg taacaatgaa ggagcaacga
    41581 aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca ttcacttgga aaacttgact
    41641 aaattagcac gactaggtgt gcaaatcata ccaccaattc ctgcttttta taatcagcct
    41701 caaacgatcc aagatcttgt taatcaccat acaatgaaac ttttggatca atttgaaatt
    41761 gtaaatgagc attcaaaacg ttggcagggt gattaatatg gaaaaagaaa cgatagttga
    41821 agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa gtaatcggta aagacttatt
    41881 gatcacttta acaggcggag atacaccaca tatcggaaca gtcactacat tttctcgtga
    41941 tacagagatg caaactgtac gtttccctag ccacgatggt cggttccata aagacgatgt
    42001 cttagccgaa aaaatagctg tgatcatcaa aactgccgtt ccgggaagtt gtacgatcac
    42061 ttccggtatc catgttgacc atatctccaa tgcacaaatc aacgcctctt ttccgatgtc
    42121 agaagagtta ggttacaaga ttttagattg gttggaaaaa atagatttcc atatcaaaga
    42181 accagtttat tataaagatg gtgaaaaacc attatgaaag aagaggttgt gaaatcagcg
    42241 ttatgacctg agtatgagag aaaaaaatgg aaaaatagct tctcatattt tgccgttttt
    42301 gagctaatac cgagggacag ctgattgaac gccgtttatc aagaggttgt gataaagccg
    42361 cttcgtttcg agtagtaaga aaaattttgt aaacatgggt ttccacattt ttgcataaat
    42421 ttgacttact accgagaaac tggcttttga acaccgttta tcaagaggtt gtgacacgtc
    42481 ttttgtcaca acctctttta tttttgcttc actgtaactg ctacaatatc accgatttct
    42541 tttccgatgt ttttacgaat atcttttcgt agtccaagga tgtagcagac tgaaccatct
    42601 ggatttttca cgcccatatt cacgatactg ccttcatacg gcacattgtc aaaagttgct
    42661 tggactttaa ctcttccagc accgaattct tttcttatat catacggaaa aataatatat
    42721 gccccgccct tgccgacttc ggaagcatag atttttgatt caaaagaata aatttccctc
    42781 attttatttc tcctattttt tacatctagt atagcatagc aaaaaaaaga aggaactgtg
    42841 acaaaagtct tgtcgcagtt ccttttttcg aataaacggt gttctcctgc caactcctcg
    42901 gtcacaagtc acaatagtca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    42961 ctcagagtta aacggcaggc tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    43021 tacggcacta agcccaatca gatgaaaaat atggagtgct atttttatct gattgttctt
    43081 actgcttgat gcagagcagc tttttcacaa cctcctacat ggtgttcaaa atattattcg
    43141 ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt ggaattcagc agctaaggct
    43201 ttcgtacgtt gttcttgctc tgctttgctc atttttggat tttcagtcat tgcttttacc
    43261 ataccgtctt gaacataagc ttttgcttca ctttccatcg tactttgctg tttcttcact
    43321 aattcagcta acttggctgc ttggtccgta cttaattcta cccatgtctc tgggattttg
    43381 aatgtattca ctcggctgtc atattcgtga ttgttatcag caatattgaa ccagaattta
    43441 tacatgtcgt ttttgtattc ccaataagta gttttcgtca ctttttcagc atcgccgtct
    43501 gtcttttcta ctttatttgt ttcgttatcc gtaccagttg cttttggttt ttcttgtttt
    43561 tcatttgttc ggtaaagata cactttttct tttccattcc ctaaagcttt atacaaaagc
    43621 atatctgctc cttgggaatc agcactggaa acaatttttt ctgtttttgt ttccgttact
    43681 ttttccattc caaagtgatt ggacaaatta gcgacgatca aacttagtga agcgacgaag
    43741 attagtccga aaatcaaaga taaaaaggtt tgccatgttt ttttagcgaa cacgttcgtt
    43801 aaagcaaaag caagcaagct gacaatcaaa actccaataa tcatgcatta tcccccgctt
    43861 tcattagaac aggttctttt ttcttcaata aaaaggcaat aataaagcca agaatcccaa
    43921 agatgatcgc tacgataaac gcggcatggt aacctgacag tgtggcattg attgcctgat
    43981 ctttataagc taatggcgta ttatccaata cagatttgct tgggatattt gcttttgtca
    44041 cattcgtcaa cacactgatc aaaatagctg taccaatcga gcttgcgatt tgacggaatg
    44101 tattatttac cgctgtccca tgactgatca agttatttgg caatgcattc ataccagaag
    44161 tcgttaccgg catcatgacc atggagatcc caaacatacg gatcgcataa aggatcacga
    44221 tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg tgcagttgcc gctgtcaaaa
    44281 tgaacatccc tgtgatggct aaacgtttcg cgccgtattt atcgaaaatc gcacccgtca
    44341 caggcatcat agcccccatg atcaatgcac ccggcaacaa catcaagcca gaatggaatg
    44401 ctgattctcc acgaatattt tgaatataca atggtacgac catttctgcc cctaccatcg
    44461 ccatatttgt tacaccagct aaaacagctg caatcgtgaa tgtagatgat ttgaatacac
    44521 gaagttccaa gaatggatgt tccaaatgta attgacgcca tgagaacaag gcgatgaaaa
    44581 tcacaccaac aactaagaaa ccaattactt tcaagcttcc ccagccgtca tctcctacac
    44641 tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga taaaatagcg gataaaatat
    44701 caatgctagg atttgacaat ggaataacac ttttcattag gaagaatgct aaaaccaaga
    44761 caagaacaac gattggcagt accataccaa acaagtcacg ccaatggtag ttatcaatga
    44821 tataaccaga taatgtaggg cctaaagcag gagcaagtcc aataacgatc cctgacatcc
    44881 ccattgctga tcctcttttt tctggcggga aaatagatag catgatattt tgcaataatg
    44941 gcatcgatac tcccacaccg caagcttgga tcaaacgacc gattagtaat gtactaaaat
    45001 taggtgctgt aaagcaagta atcgttccga tcaaaaagat cgtcatggca gaaagatata
    45061 aatttcttga actgaattta ttgatcagcc atgctgttat tgggatcatg atcccgttga
    45121 ctagcaagaa accagtcgtt aaccattgca catcagacgc tgaaatatcg aaagctttca
    45181 tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa aacggtacaa aatgtaccaa
    45241 ttaataagac aacaactaaa agtgtccgat tatatggttt tccgtaaata tctacgggtt
    45301 ggttgttact catatttctc cttctttctg aattttttct gaaactacta gaatacttta
    45361 gtcctaaatt cgctatttgt caacctactt gacacataag ttaatacgga tataattaag
    45421 ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg cttcccttaa acgtttgttg
    45481 gaaaatgatc aattggttgt tggcatcagt gaattaagtg atatcgtagg tgtgtctcca
    45541 cgtcaattaa ggtattggga acaaaaggga ttcattcgtt cgatcgaagc tgatgcgaat
    45601 tgtcctagaa aatatcgttt acctacagtc ataaaagtag aactgatcaa aaaatattta
    45661 gatgacggat ttactttgtc aaaagctgca gaaaaggcag aaatccgcca aaagaaaatg
    45721 caccatgtcc gtaaagtttt ttcaaaagca atcaaagata tcgaattgat cgacgaacgc
    45781 tatacgattt tacaaatcgg taacttcaaa gaagagaatg ctaagatgta catcattcat
    45841 gacaacgaga cagacgaatt aacttacaag ctccttcctg tcgatgaaaa agctgactac
    45901 gaacagttgt taaaagaact ctaaacgcca ctaatctcct aagtaccaag ctttttaata
    45961 agtagatgct acataaatac ttgaaaaaag gcctgtttga acataagaat atccgaacgg
    46021 tgatctgcgt ttctttcata tagaaaggaa ttctccatca ctgttcggat atttttttat
    46081 ttttttcggt tactttcttc taatacatct gttagccaat ttgcatattc tgtttcccga
    46141 ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt catgtttttc tttttcagta
    46201 ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt tttcttcatg aaggtcacgc
    46261 tgctgctgaa taatttctac taatgccgga tcattcacgt ctttgacgaa atacaatttc
    46321 agaacaaatt cgtctcgatt aaccggtagt tcattggtgg gtgtatgaat ccactctgcc
    46381 aattgttcct tcccttcttc tgttaattca taaactttct tttccaattt ttcacctgtg
    46441 atctctactt gatgctgaat gattccttgc tcttccattt ttgcaagttc aggataaatc
    46501 tgactatgtt ttgcttgcca aaattctcct atttcatttt tgaacgattg attcaattca
    46561 tatcctgtca tcttcttctt acttaataac cctaacaaaa tatatcgaag tgtatttttt
    46621 cttgccatca gttttccctc cttgacaaat ctttaaatcc attctatcat aaatgagtaa
    46681 tcgaaaacat gtagtttatt acatgtttta atttataagg agatgcttaa acatgaaaga
    46741 atttaaagaa ttggaagatt ttattggaac tcactttatc tatacgtatg acaatggttg
    46801 ggaatatgaa tggtatgcca aaaatgacca tactgttgat taccgtatcc atggcggaat
    46861 ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc gttaaattga cggacggcgt
    46921 attcaaaatc acttggacag agccaacagg gacagacgtg gcattggact ttatgccaaa
    46981 tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg gttgaagaac atccagaaat
    47041 cactgttact ttccaaaacg aacatatcgc agaaatggaa gcagctcgtg aaaaatatga
    47101 aacttatcca aaattacttg ttcctgaatt tgctacgatc acttatatgg gtgatgcagg
    47161 tatagacaat gaagatgtta ttagcgaaga accatacgat ggaatgccag atgatatccg
    47221 tagcggaaaa tacttcgatg aaaattataa acgagtaaaa taaggttgtg aaagagaggt
    47281 tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa cggaaaaata gttcctcatg
    47341 ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcaagagg
    47401 ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta gttagaaaat agcttttcat
    47461 attttctagg ctagtgggct tattaccgag aagcagccgt ttgaacaccg tttatcaaga
    47521 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaatggaaaa atagttcctc
    47581 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaaa
    47641 gattgtaaca actgcatatt cacacagtta aattagtgac ttcaaaatga aataatgctc
    47701 ccagcaaaaa attaggctgg gagcattatt tcgttattct gttcatcaaa aatcagcgtt
    47761 ttgattccat ttcatcacaa gttcctcctc atttactgct tcatcttttt gctgtgtttg
    47821 aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat tgattttttt gatagactgt
    47881 caaagtcagt gttttattgg tttcttttat ttttttcatt agttgcgtac cgattctttg
    47941 ttgccggtat tcccttttca caaagattcc tgcgataaac tgatcaatca ccccagcaaa
    48001 accaataatt tcttgatcta tttctgctaa gtaaatcact gcatctggta aagctttcct
    48061 aacagattct acttgattct cccaataagt tgatggaatg aattcatgag aatccaagtt
    48121 agcatgcagc caaatcgata gaacagattc cagttctttg gcagacaatt ttgttgttgt
    48181 gcggatcatt ttttctctcc ttacttattc attgatttta aaataaaaac catttttttg
    48241 aagctctttt acaagttttc gcccctcaaa aaagtatatc ttctcttgtt ttacttttct
    48301 aataccaaaa gaaacagcaa ctttaggatc atcagctgaa acgattcgat aagttccacc
    48361 aggcaattga tccagcttga ctttcccatg atggtcgctt tgtactgaaa acggcacacc
    48421 atttctagta agtgtttttt tcttatgttt caaaatgaat gaactaccat ttaccggttt
    48481 ccctgacgaa tcactaataa taaatgaaaa atcaagcact tttttactta atctatactg
    48541 atagattagt cgtccgataa acactaacag tatgactaca gctattatcg ttcctgcaag
    48601 aataaaccat tgccgcatgg ttctttcctg atttcctttt ccttcttctt tttttaccga
    48661 ttcgttataa ggaatccgat gtcctgtcac tagcaaacga tgcgtgttga tcatatacgg
    48721 cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa atcgttagaa cagacgtgtc
    48781 atctggcagt accacttgta tatttttaac ttgataagcc aatttctccc ccaaaacagt
    48841 caaaataaac gtgtctccgt tttctaaacg atctaattcg gtaaataacc gtcgattagg
    48901 taaaccgctg tgagcagaga tcactgaatg cgtcgaattt ccaccaagcg ggaaagaggt
    48961 tccttgcaag acgcctgctc cattttcaag gatcctacca ttgagccgat cataaagtga
    49021 aatatttaat tggatcttag gtatattgat acttccgatc agccattcct ctaattggct
    49081 ggatgttgta ctttcatttc tgttcttttc attgaaagga tcagtaccag gattgaatcc
    49141 ttggtcagtt aatcgttggt ttttctcctc cattttcttt cgctgcactt caatttcagc
    49201 tgcggtccgt ttttgtactt gttcgatacg tttttgatca ataaaactat ttaaagaatc
    49261 cacataaaat ggatataaag cgatcagtaa ccctacgacg atacatatta ataaaaacaa
    49321 acgaaacaaa agtcgacgag actgtttttg cttactctgc ttcacatgtg tcctcccttg
    49381 caagtttttt tgttctttct tttattatat gggttcagga aaaaaataga aaatgaaagc
    49441 cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa gtacagccca aaaaaatagc
    49501 tactcatatt ttttgttggg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    49561 taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa taagagcgct agtcagaaaa
    49621 tagcttctca tattttctag gctagtgagc ttattaccga gaagcagctg attgaacacc
    49681 gtttaccgag aggtcatgaa aaagctactt tgtttcgagt attaagaaaa attctgtttc
    49741 aaaacaagga gatgcaacaa aacttatgtc gcatcctctc tccttagaac tttcacttat
    49801 gtttttcttg taaaattcat aaatattttc atgaattttc taatttcttg cctacctctc
    49861 gcataggata tatgatgaat ttatctaaag tttgcaaagg agttcgacaa ttgaaaaaat
    49921 atggcccatt tttagcaatt tttttcattg ctttggcatt tctcatgcct catttattca
    49981 cacataaact aattttgggt gctgatgccc cattccatta caatcggttc tatgatgccg
    50041 cgcaacaaat taaatatggg gatttccatt attttccttc cctttatgga ttccagcaaa
    50101 gtggcaggat cgtgaatgcc gtttacggcc catttttcgc ttatttacaa ggattgatcg
    50161 tgttgcttgc aggtagctgg tttcattatc agattctttc aaatttcatt ctttatctta
    50221 tttcaggatt ttctatgctg attttactga gatatgtcaa tgttcgaaat tgggtaagtg
    50281 taccagtagc cgttttattc atgagtacgt attctattca atactggtct atcaaccaag
    50341 gattcactag ctgggggact tgtttttatc ctttatgtat gatccctatt gctgatttcg
    50401 tccttcataa aaagttccct gttatcaaag tcgctgtctc agtgggactt atgacacaga
    50461 tccatttact gtcttcgatc atgttagctt tgatgtatat ccctttttat ctttattact
    50521 tttttaacca gactgaaaaa ggaaaagcac tgcttcaact gattaagtcg gtcttgcttt
    50581 attttgttct gacagcaaat atttgggcag gattagctat tgtttacagt gggaataaga
    50641 ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc gatagatctg gatggcagtt
    50701 attggctcca ttaccctaag gcctttccta tattgatatt atcaggtttt ttgcttttta
    50761 ttttctttca acaaaagaac accttattcc aaaaaatcgt gttgtacaca agtcttgttt
    50821 tcttttttct ttctactagc ttgattccat ggacgacatt gatcgaaaaa aatgtgcctt
    50881 ttattagttt gatccagttt ccatttcgat ttttcgtgcc atttacgcta ttgcttctac
    50941 tttaccttgc cctttcactg aaccagtggt ctgaacaaaa gtggttccgt cttttaagta
    51001 tatttggcat tcttattttt tcagcacaga cgatccagaa cttataccag catctggaaa
    51061 agtgggaaaa tgaaacattt gtcagtcgac atacttatct attcgatacg ccggaagaag
    51121 caaggaaaac attcttcagc aaagatatgt ctaccagctt gttcgttttc caaaaaacca
    51181 cacctgacta tctgccaatc tataaggaga caaaagacaa taaatacgac cggtataacg
    51241 aagaaatact agaaaacgaa acaactttta taaaaacaca tccaggcggt cttttgacaa
    51301 tcaagtggaa aaacactggg gacaaaactg ttcatatccc tgttatcgtc tacgatcgga
    51361 ctgtgctaca gcaaaatgga aaaacattga ctgattatga agtaacagat atcgggaccc
    51421 caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct tcattatcaa acgccaatct
    51481 atttctattt cattctttct cttactttga ttggctggtt taccttgcta tgtttattta
    51541 tttatcatag atacaagctt ttacgtgctt aggcaaaagg gataaataca tccgatcaaa
    51601 aaactctcca aacggtataa tcgtttggag agttttattt taatcagttt ttttatgtta
    51661 tatgttatat tttttattcg ttcacatcta attcgatttc caccccgtag tgatcactga
    51721 tgattggact atcgatccca tcaaaaatcc gttgatattt tttaacctgc attccttcag
    51781 ggacgaaaat aaaatctata cgcaattttt ctgtattttg ttcccaacca tctattttct
    51841 tttccacagt agctgctttt cctttttcat tcgcgacaac atacgaatct tgaagaagaa
    51901 ggcaactttc tcttacagta taataacctt gagagtctac tggattattg aaatcaccta
    51961 ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc caagtttttc cactcaaaat
    52021 aaaagccact ttttttatct gtccaccaag aaaagtggca gctcgagaca aaaatgtcct
    52081 gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt ctgataatct tctttctttt
    52141 gaaattggga gacggtaagg acctgacttt ccaatgtttt tttcgtaagc agtgcatttc
    52201 cttcttcata tttatcataa ccgatgtggc tcatttccca actccaaaaa tactcttttc
    52261 cgtgttcctt taaaagctga actaaacggt aggcaaaatt atctttacgg ataggtactt
    52321 gattctttac cggacagaac ttcattagct tcgcaggttc tacttcttca gattccagta
    52381 attggttgat ttcttgtaaa gcaatcactt cataatcttc taacaggatc ttatctgcta
    52441 attcttgcaa ttttttctct ggttctggct caagccagct atgcgtgttg agtgtcatga
    52501 atttcattgg ttttctcctt gtcttcattt cgaaacaaaa gggttgtgac aaaatttttg
    52561 tcacaacctc tccagcaaat agtgttctcc tgctaactcc tcagtaataa gctcaatgga
    52621 ccaggagcta ttttttgttt ggctgttttt atcacttggt gcagggcagc tttttcacga
    52681 cctctccact aaacagtatt caaactgctg cttctcggta ataagcccaa ccaaacgaaa
    52741 aataggagga gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    52801 caacctctcc ttacatttta atggagccga tcactttacc agctgtcact gtacctaatt
    52861 gcttgatcac tacttcttct actttatcgt ttgtaaatac gatcatgatc gttggatctt
    52921 tttcttcgtt ttggatcaca tcaagatcca tctcagctaa tttcgttcct gttcctactt
    52981 cttggccttc tttgacagca atctcaaaag ctggctgatt catttggact gtatcgattc
    53041 ccatatggat caaaacttcg ataccatctt tcgtttccag cccaattgca tgtttgctag
    53101 ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac ttctcttgat gttggaataa
    53161 cagcaaagcc gtcacccatc attttttgtg agaataccgg atcttttact tcagataaag
    53221 gaatcacttc tccagaagct gcggaaacta gttcttgttc tatacctaag tattgcttac
    53281 tacctgcttg tccatttcct gtagaaacca tttctggctc tggaatctct gctcctgatt
    53341 gcagcagatc ttcgatatct gatttcaaga catctgcttt tggtccataa actgcttgga
    53401 ctccattgtc tttgacgatc aatcccatcg cccctgctcg tttccaggct tcttcagtcc
    53461 ctactttttc tcgatcattt acacttacgc gtaaccgagt catacaagca tcgacatctt
    53521 tgatattttc ttttcctccc agcaaatgaa tgatctggac cacttgctga tctgctgttc
    53581 ctgttccgct gttagataca acggtttctt cactgtcatt gtcgtaattt ccgttacgtc
    53641 ccggtgtagc aaagttgaat tttttgatca agaaatttgc taagaaatac gtaacaacac
    53701 caaaagcgat cacacacaac acgaagttga taagatctcc acctagaccc gctttgatag
    53761 ctaacggtgt ccgagtcagc aattcgatat tcccaaatga atggacccgc aatggcaaga
    53821 tatctgccat cgcaaatgca gccccttgga tcactgaata aatgacatat aatggcacag
    53881 cagcaaacat aaacatgaac tccaatggtt ctgttacacc tgttagaaag acagccaacg
    53941 ctgctgaaaa atacattgat ttatatcttg ctttcttatc aggatcgaca ttacgataca
    54001 tcgcaaaagc cattcccatc aagatacctg aagaaccaat catttgtcct actttgaaac
    54061 gagccggcgt ccagttatgc aacacaaact catattgcga catatcccct gcacctttaa
    54121 gattcactaa atcagtggcc caagccaacc ataacggatc ttgcccaaat acttgtgtcc
    54181 ccgcctgtgc gcctgataaa atctcatacg atccgccaag ctgtgtatag ttgataggaa
    54241 tcgtcagcat atgatgcaat ccaaatggca atagtaaacg ttctaatgtt ccaaataaaa
    54301 acggtgctag aatcggtgcg ctttcttgtg attctgcaat ccataaacca aaattattga
    54361 ttccagcctg tacgtttggc cacaccacag caagaatcaa tgccacgatt gttgaccata
    54421 agataacaac aaacggaaca aagcgctttc cattgaaaaa tgacaaagca tccggcaatt
    54481 ttctataatt atagtactta ttataagcca ttgccccgac aaatccagcg atgatcccta
    54541 cgaatactcc catattcaaa gctggtgctt ccaatacact tgtaaaaaat cctttaacca
    54601 tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc attcgcaagc atgtcacttg
    54661 tgacaccaaa gatagcccca gtgatccgat tgattaaaat aaatgagatc ccagctgcaa
    54721 atgctccgcc tgctctttct tttgcccaac ttcctcctat tgctaaagca aataaaatat
    54781 gaagattacc aataattgcc caaccaatat tttcaattac tccgcccgtt gtgacaagcg
    54841 gcgtccaatc aggattgatc aagggaattg atttccctat actgatcatc aacccagctg
    54901 ccggcataac tgctaccaca accattaaag cctttccaaa tttttgccaa aactcaaaac
    54961 taaatatctt tttcattccg tttagctcct ttacgcaatc ggtttcgtta aacgtattat
    55021 agcgtgcgtt ttcttttttg taaatgcttt attttttatt tttctgtcta taccattcta
    55081 taaaacctat aatacgtgtg ttcccttcta taattttctc ctaaaaattt atttttttaa
    55141 gcttttttct atgaaatatt ttcttttgtc tattgacttg tgcaatcggt tgcgtttatt
    55201 atgaacgtat aaaacagctc attaggagga gaaaaatgaa acagatcaaa cgactatttg
    55261 ctattgatcc atggaaaatc agcacgacac aattagataa aaaaaatctt cgactgcaag
    55321 aatctttaac aagtattgga aacggttata tggggatgcg cgggaacttt gaagaaactt
    55381 actcagggga ccaccatcaa ggtacttatt tggcgggtgt ctggtatccc gataagacac
    55441 gtgtcggctg gtggaaaaat ggttatccag aatactttgg aaaagtgatc aacgccttga
    55501 actttatcgc gatagatatt tcgatcaacg atcagctgat cgacttggct tctttggaac
    55561 cagaagactt ttactgggaa ttagatatga aaaatggtgt attatctcgt acattcacaa
    55621 tcactacttc tactaataaa gtacgcattt ccttcgaacg ttttctgagc attgtcaaaa
    55681 aagaatcagc ttatatctat ttaactatag aaatgcttga aggtagcgga acagtcaaac
    55741 tcgtatcaaa attggatgga aatgttcaaa acgaagacag caactatgat gaacattttt
    55801 ggaatgagat tgatcgtgga actatagaag aaatcggcta tttgacgact aaaactattc
    55861 caaacaactt tgggattgaa caatttactg ttactgcagc aatgcgccat ttcgttaatg
    55921 gaaaatcagt catccctgac tatcacgaag atactttagc attacatggt tgcttgtctg
    55981 cagaattatc tcaaggtgag acatggtcag tggaaaaaga aattatcgtt cttactagta
    56041 gagatattcc agaagcgcag caagcagaaa aagctgtctc agaattaacg aaccagacaa
    56101 acaattatat ccaagcaaaa gaagagcaaa cagcggcttg ggcagaacga tggaagcttg
    56161 ccgatgtggt catcgaagga gacgaagaag cacaacaagg tattcgattc aatttgttcc
    56221 aactattctc tacttactac ggcgaagatg aacgattgaa tatcggcccc aaaggattca
    56281 caggagaaaa atacggcgga gccacttact gggatacaga agcgtatgct gtccctcttt
    56341 atcttgcttt agccaaacct gaagtaacta agaatctgct gaaatatcgg cataatcaac
    56401 tgccgcaagc aatccacaac gctcagcaac aaggattaaa aggcgcgctt tatcctatgg
    56461 tcaccttcac tggtgtcgaa tgccataacg agtgggaaat cacattcgag gagattcacc
    56521 gaaatggcgc gattgcctat gcaatctaca attatacgaa ttacaccggc gatacggaat
    56581 atttgaaaaa tgaaggactg gaagttttag cagaaatcgc acgcttctgg gcagaccgtg
    56641 tccatttttc aaaacgagca aataaataca tgatccacgg tgtgaccggc ccaaacgaat
    56701 atgagaacaa tatcaataac aattggtata caaatacgat cgcagcttgg gtcttgcgtt
    56761 acacgcgaga aaattattta acttatcaaa aagaagctgc tatcactatc agcgaagaag
    56821 aactagccaa atggaaagat atcgcagaga acatgtactt cccagttgac gaagaattag
    56881 gaatctttgt ccagcatgac acattcttag ataaagatct catgcctgta tccgatattc
    56941 ccacaagcga tcttcctctg aatcagcatt ggtcttggga caaaatcctt agatcttgct
    57001 tcatcaagca ggcagatgta ctacagggga tttatttctt caatgatact tttacaaaag
    57061 aagaaaagca aaaaaacttt gatttctatg aaccaatgac tgtccatgag tcttcccttt
    57121 cgccaagtat ccattcgatt ttagcagcag aattaggaat ggaaagcaaa gctgtagaaa
    57181 tgtaccagcg aacagctcgc ttggatttgg ataattacaa taacgacaca gaagacggct
    57241 tgcacatcac ttccatgact ggtagctggt tgtcgattgt ccaaggattt gctcaaatga
    57301 aaactgccca tgatcagttg agattcgcac catttctgcc aaaaacttgg aaagcttatt
    57361 ctttccatat caattatcgc ggacgtttat tgttcatctc agtcacagat gaacaagtta
    57421 ctttcgagtt attagaaggt tctcctctag aaatttacgt ctatgaacag tcttattctt
    57481 tagcggatcg tctagaaata acgctaagaa aggaggaagc aaatgtttaa aggggtattg
    57541 tttgatttag acggtgtcat taccgatact gctgaattcc attatcgtgc ttggcgaaag
    57601 cttggtcagg aaatcggcat tacgatcgac cacacattca acgaacagct aaaaggcgtc
    57661 agccgagaag actcactctc tttgctttta gcttatggcg gaaaagaaca ctctttttca
    57721 aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt attatttaga gatgatccag
    57781 acgatcgaac caaaagatgt cttccccggg gtcgtgcctt tactggattc tttaaaagaa
    57841 gaaaaaatca agattgctct tgcttctgca agtaaaaacg ggccattcct acttgaaaaa
    57901 atgggtctta caccttactt tgatgctatt gccgacccgg cagaagcaac aaacggaaaa
    57961 cctgcgccag atatcttcct ccttgcagca aaagctgtcg gactaaaagc agaagaatgt
    58021 atcgggatcg aagacgcgca agccggtatc caagccatcc tctcaagtgg cgctcaacca
    58081 attggcgtgg gacgtgctga agaccttgga gaggagatcc cgcttgtccc tgatacgact
    58141 tttcttacga tcgattactt gaaagaaaag tggcatgacc atggataatc cagagatctc
    58201 cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca ttgaagaacg actacttaga
    58261 agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc ttcgcaccag acaaagaagg
    58321 aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa gatgttctaa cagacaaaag
    58381 cttttttgga gcaactgtcg gacctgtagc cggaaggatc cgggatgctt cttggggtga
    58441 gcatagattg gagaaaaact gcggatcaca ccatatccac ggtggcacga aaggctggtc
    58501 tttccaattt tgggaagtag aagttttcaa aactccatca tcaataggtg tggtctttta
    58561 cttaaaagat gaattttcta cctatccagg accaatcact gctacgatca cttaccgttt
    58621 aacaaaaaac gaattagaga tgacaacctc tgctagtagc cttgttgaaa ccatctgcaa
    58681 tccgaccaac catgcctact tcaacctttc aggaaatgga acaagagaca tctatgaaca
    58741 tcagttaacg gtctttttag atggtgtgct tgaattagat caagaaaaac tgcctactgg
    58801 aaattggaca aaaaaagaaa aactgccaat cgactttaga aaatccccaa cactgcaaga
    58861 gatccttgca tgttatccag atggactaga tgatgtattt cttttgcatc atcctaggct
    58921 ttccaaaact tccttacaat tattcgagaa gcattctggt cgccaaatga ctatagcaac
    58981 ttctaataaa agtatggtat tattttctac gactggattc gaagctgatt tctctgtcaa
    59041 tggaaaacag atgcattcaa actacggatt agcaatcgaa ccgcaagaaa tccctgatat
    59101 cgttcatttc cccaaatttg gatcgatcaa cctccatcct ggtcaagaac ggatcagtca
    59161 gacgatttat cggttctctg cacaataaca agagaaaggt gtttttatca tggctattac
    59221 cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg accgtctctc gtgtcatcaa
    59281 cgatcatcct agtatttccg agacaacaaa gaagaaagtc cgaaaaatta tggatgaact
    59341 aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg
    59401 cgtaatctta ccccctcttg attcaaaaga gcggatcggt aacccatttt atcttgagat
    59461 catggaagca atcaacgaag aagcgcggaa ttatgatatg actacagcaa tcgctactgc
    59521 taaaaatttt gaattgctgt tagagaacgt ccagcgtatg catttacaaa aacaagtaga
    59581 tggttttatt ctagtgtatt cagatagtca agatactgtc attgattatt tataccaaaa
    59641 ccacatccct ttcactttga tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt
    59701 agataatgat aaccagcttc taggtaaaca agcaacagaa tttttgatcc agaatggaca
    59761 taagaatatc ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg aacgatattt
    59821 cggttatcaa aaagcaatga tgatggctgg tcttcctgcg ttatccagtg tagtactaga
    59881 gcatccggag gattatctgt cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt
    59941 ggtggtcatt gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa tgtatggcta
    60001 tgtcattcca gagacactct ccgtcattag cttcaacaac tcgatcttct caacattaac
    60061 acacccttac cttacaagta tcgatatcga tatctccgaa ttaggacgtg tcgctatgca
    60121 aaagctgcgc gaattgatcc atgaagaatt gagcagtggc gtacgtttgg tcatccctca
    60181 tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac ccatactaaa tagctgactc
    60241 actaaatacg ctgaccattt atcctatggt cagcttttta tgtaaaagaa gatactactt
    60301 ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac acttttgtct
    60361 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa
    60421 attcacaaaa atttaaaaag caattttctt gagtttcttc ttatttcttg gagttaaacg
    60481 cttctgtccc agcctctttt ttccttcctt caatttttta cacgtcgacg attcaataaa
    60541 aaaccgccta atattgtaaa gacaggtgca agaatcgaga aaaaggcgaa tggcaagtaa
    60601 tgcaacgcgc taactttcat cgttcccata atgaatgtgc cgctgactcc ccacggtacg
    60661 agagaattga cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt ttttgataag
    60721 ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa gaatgatgga cagatactgt
    60781 tctccaacga tcagattgat tccgatacag ctcaaggcag taaacccgac taaacgagaa
    60841 ggtcggtcca tcttttcttt taattcttga acgatggtat caacgatcag atatttgatc
    60901 agtaacccac ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa catactcatg
    60961 atcccgcctc gagaaagcag ttcatctagc tttggatcag gtgtatttgc gacataacca
    61021 ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt tctcatggat aaaagaaaga
    61081 acgagagcag ctgtcgatcc cgctagtaat gtagggatag ccggaatttt tttgattgcc
    61141 cccccaaata aaataactag tggaatcaat gtccaaggag agatccaaaa gctcgctttc
    61201 atgttttgga tcatttcatt gatactagct agatcggctg aatgactgtt atgtcctaat
    61261 atcgtataaa aaatcaacga aaggacgaat gctgggaaat ccgtataacg cataccagcg
    61321 atatgttcga atagattaac tcctccaata cccgctgcca agttggtcgt atctgataaa
    61381 ggcgagcagt tattccctaa aaaggcaccg gaaacgactg cgcctgtagt cattgcatca
    61441 ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc caatcgtcga aatcgttgta
    61501 aatgaactgc cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag
    61561 aaacgcacag aaacaagttt aaatccataa accataatcg tagggatggt tccagataaa
    61621 atccatgaag caactaagac accaatcaaa agaaaaatca ggattggaat gatccctgga
    61681 gtgattcctt tgatgatccc gtcatggatc tcatcccatg tgaacttttt aaatctgcca
    61741 taaaacaaca aacacatgaa agctgcaagg atcgggatat gtggcgagag ctggaatccg
    61801 atgatcagta ctcctaggag tgccaacaac acgatcaata cagcgatact ttcttttaaa
    61861 gaaatatttt tattcatcgt cgatctccta tttcaaatga aaaggccctg ccatgaaaaa
    61921 actattcact tggcaggacc tcctactaaa tcagcgacaa aacgtcggct gaaagactta
    61981 tttttttatt tttcttctaa acttttttct acctcaatcc cccaagaatc tttcttgaag
    62041 tgattgatga tcaatggaat aacgaacatg accactgtga tagctgctac taataatgca
    62101 tcactcaatg tcgctgtacc taatgtagca aatacgacaa cgatgataga gaagatcagc
    62161 atgcagctga ctacccaagc taatccgttt ccgtttttac caattcggta aggacgttcg
    62221 aattctggat gtttcttacg cattttaagc aatgaaacag caatcaaaat gtacacgata
    62281 caataaataa cagttgtagc atttgtaagt gtcaagaata cagcacttac gtcatccatt
    62341 acaccataaa gcaaagcaaa taatgaaata acgattgatt gtgttagaac gacatttcta
    62401 gatacgccgt atttgttttc tttatggaat ccgaattttg gtggaaggaa tccttcacga
    62461 gctacttgga tgattgtttt tgatggtcca gtaacccaag ctgataattg taaaagcaca
    62521 ccaataaata ccatgaagct gaagatattt ccgatcactt ctggtaagcc taggatttga
    62581 cagtcaatca agataggctg cgtgatattt gctaattcca tttttccatc tggtacgaca
    62641 tttgctacaa gcatcgcatt gatgacattc aataaaacta atccgatcaa agcgataaat
    62701 acgccttttg tataattttt tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt
    62761 tcgatcccaa caaaaataaa tgtgatacca gctaagtatt tcaatgaatc aatatgttcc
    62821 aagtcaggga aagcttttga ccatgaaaag tcacctagat agccgtttga gactagtccg
    62881 actttgaagg ctgccaaaac acctaaaacg aacatgatga ctactgggat atatacccct
    62941 agccatacac cgatgttccc gccgactttt gccatatcga atttcaagtt caagatcgtg
    63001 atgatccagt agataactaa aatacaacct aaaacgaacc agtgattatt gccaagttct
    63061 acattaccaa atgtgtttcc taacaatggt cctaaagtag aagcgaccat taccatacct
    63121 gggaacattt gcacccataa tagccatgca acgacaaatc cccatttgct gcccagtgct
    63181 gtttttaccc ataactgagg accgccttct tgaggaagca tcgtggaaag ctcaccggaa
    63241 accattgata tcggaccggc aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc
    63301 caaccgactg cggctaacgt aggaatactc cgaaccgttg cacacagtgc cattgtcaga
    63361 ccgataaagg tcccaagacc cattttactt acttcagatt tactcaccgt tgacactctc
    63421 cttgattttt tggataaaat aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa
    63481 ttcagcgaat atttgagggg attctttgag tttattgctt gaagggtttc ttcaaagaca
    63541 acctcaaaac aaagggatcg aagagcttag gtctcccctt gctcttcaga tccaaacatg
    63601 cgtcagaaac agttagtatt caagctgttt tctaacatga ttaccttgct tattttgctt
    63661 cgcttgccaa taattggtct gcgtagattt tttccaattt ttcttgaaga gctgctttga
    63721 ttttttcagc atattcttca aagtgttctt ctttgttcat gtatggtgtc attacgctgg
    63781 cacgcaatac agttactttt ccggcacggt tccattcttc atctgagaat cctagttggt
    63841 tcacaaattg taatgggctg ttgccatagt ctgggatagc aaagtcagta tgtgaagtta
    63901 agaactcatt accgtaaatg cttcctttca cgtatgaaga ataatcatat acgtcatggt
    63961 tcaatttatt cattgcaact aagtcatcgt tgcctttttc tttgaataca tagtctacca
    64021 tattgaaatc tggataagtt aatggatgaa cttcgatttc tttgtctcct actttaaatg
    64081 acaagtcatt caagaagttg tagaaacgat gtgcaccttc gattgatgca cccattaatt
    64141 taccatatcc tgtgacattc aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg
    64201 ttgcccctgc ttttgaacct tccaagatat aagccccaag taatgctggg atatctgcgc
    64261 ctttttcaaa tacatatgtc gcaaagtaag agataacgtc tctcatgcgg atatctttga
    64321 tgacgatacc accggcagaa tatggaacat agcccatttt atgtgggtca atcgttacag
    64381 attctgcttc ttcgattgct ttatatgcac tgtgtacttc ttctaagata taatctttgt
    64441 tttctgtaaa gacattgtat ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt
    64501 tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc ataagcagca tctacgtgta
    64561 agtagaagta aataccgtct ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac
    64621 cgtcgattgc tccttcttct gttgatccta caacaccgac tacacctagg attggtgttt
    64681 tttcagctgc tagaccacga acgatttttt caagttcgtt gatgtccatg cgatagttgt
    64741 ggtcaactgg cactggaatc acttggtcta aaccgatacc gatgatgtcg gcagctttta
    64801 gccatgaata gtgtttagtt tgtggaacca accattttcc taatttttct aaatgttttc
    64861 cgctacgtgc tgagtgagct ttgatttcgt cgattttttc tgggacgctg tctaacagat
    64921 tcatgatttc ttctgttgac aagttcatta attcccaatc gctttttcca gcaactagtt
    64981 caggtgtcac ttctttcatt gcaagcggca atgatttgat gttgcgggca taccaaagtc
    65041 cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc ccagccatct ttatagctca
    65101 ttaattttgc aaattccatt cctacttctt cttccatttg gcttgttgca ggagaagatt
    65161 cataggcaac gttattacca ttccatagca tagcaaaatt gtacgctagt aaagatggca
    65221 tcaatgtttc agagttcata tgaccccagt aacgtccggc attatgccaa ggaacggaat
    65281 gggtacgcat ccgcgctgaa atttctgata gaacatcctt tgttttattc acagtgtgtt
    65341 caaaactagc actgcttttt tcttctggcg taataatagg catgtcttga ggcatgtagt
    65401 tttgacgcca gcctaaatgt tcatccacca attcattcaa caaagcttta tagatttggc
    65461 cgttctcagc tttgtcccca atgaataggg catttaggtt tagatctttc gacaatgatt
    65521 cactcatagt gaaaccctcc taaaaaaata tttaaatttt gtcgagccct acattacacc
    65581 tgttcttcta ttctgtaaac taagttttgg cctgattttt aatcatattt taatatgtat
    65641 caattatccg aattgttcac acttttgaaa taattaaaaa aaacgaggga atttacaaat
    65701 gtaaactccc tcgcaaagat tgtattaacg acaaccagcg ttctatttta ggatgggttt
    65761 caaaattgga atgttgtaat agtatttccc tttttcaagg taaaaactta ctaaattcta
    65821 atcaattact ttggccaaaa agtaattttt cttacctttt cgaataacca caaacttacc
    65881 atcgaattca tcggatggat ttatgacaaa atttaaatcg gtaacgcggt caccgttaat
    65941 acttatagct ccgtttgaaa catcttcacg tgcttgacgt ttagatggtt caatttttgt
    66001 ggaaacaagc agttccacga tattttcagg tgtgcttgaa atttcaacgt tcggcatttt
    66061 accgaatcct tgcgcgattt cttccgcatt tagttcttta atatttccag agaacaatgc
    66121 ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc
    66181 ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct gtctctactt ttttcgctaa
    66241 gtcttcgatt tcttcttgag acaagaaagt aaagaatttc aagtatttca ctacatcgcg
    66301 atcatcttga ttcaaccaga attggtagaa ctcaaatggt gaagttttct tcggatcaag
    66361 ccaaacggca ccgcctgcag tttttccgaa tttcgttccg tcagccttca gcatcaatgg
    66421 gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat tttcggatta ggtctaatcc
    66481 agctgtgata tttccccatt ggtctgctcc accgatttgc aattgcacat cgtatgtttt
    66541 atgcagatgc aaaaagtcga tagattgcag gatttggtaa gtaaactctg taaatgagat
    66601 tcctacttcc aaacggctgg caacgatatc ttttgctaac atcgtgttga tgttgaagtt
    66661 tttaccgtag tctctcaaaa attcaagtaa agagattttt gacaaccagt cgtagttgtt
    66721 cacaaatgtg atatttgcat ctttaccaaa caattttctc atttgattgg aaagtgcgtc
    66781 cacgttatgt tgcacttgtt ccatcgtttg caacacacgt tctgttttgc ggccgcttgg
    66841 gtcgccgatc gttcctgttc cgccgccaat cagaatatat ggatgatggc ctgccaattg
    66901 gaatcttttc atcatcataa atggaatcaa atgtccgata tgcatcgaat caccagttgg
    66961 atcaacgccg caataaagtg aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc
    67021 gtttgtttgc tgattaatgg cgtcgcgcca agttaattca tcaataatat tcattttact
    67081 gacctccgtg tagttttttt atgaaagcag gaacctgcta gcatatgtaa ttaattattt
    67141 cttcaggata ggataggaaa taaaaaaagt cccttggcat ataagccaag ggacgaaatc
    67201 tcgcggtacc acccaatttg tatgtctaga tatccaacat acctctcgaa catttaacgc
    67261 tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa ttcgctttaa agcatgtact
    67321 ggtttgcagc gaccaccagc tttctgaaac attggcttta acaactactt cgtttcgatc
    67381 ttcgtcttgt tattattaat atgtgtctat catagagcgc aactttttct ttgtcaacag
    67441 aaaaaggata ttttttttaa attcctcatt ttttcttttt tgaatccgct ctaattccga
    67501 acattttatt ttcaattatt gttaaaagaa ataacttttt ctgtattttt tctgcaattt
    67561 atcgagaagt cactttactt tgcctcttat tctttcttcg ttacaataag gaatgggaca
    67621 agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa aaaagcaaaa aattatttta
    67681 ggagccactt ctgctttaac tatcgttatt ttaattctaa gtttgttttt tgtcatccga
    67741 tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa taacagaaaa agcaaaagaa
    67801 ccgacagatg ataagcaaca actagttact caagcaaaaa atctggctca aagctacgat
    67861 tatgaccaag cgattgcgtt attggaaaaa gatagtaaga acggatctac tgaaacacaa
    67921 cagcttcttc aaacactaaa aaaggagaaa gaacaactga tcgactggtc tgatcctact
    67981 cagatctctc atgtcttttt ccacagcttg atcgtggacc cgcaaaaagc cttccactct
    68041 caacaggcac aaggatacaa agattatatg gtgactgttg aagaattcaa ccgttcgatc
    68101 gaacaacttt atcaaaatga ttttgtttta gttaatttga atgatttgat ccaaaaagac
    68161 gaacaaggaa atctttcctt taacgggctt gcgctacctg aaggaaaaaa accattggtt
    68221 ctttcacaag atgatgtcag ctattatgaa tatatggata attccggttt tcctagccga
    68281 ttggtcgtat caaataacaa tgaaatcaaa aatgtctata aagagaaaga taaagaagaa
    68341 atcggtgact ttgacatggt tcctctgatc gatgcctttg tcaagaaaca tcctgatttt
    68401 tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt acaacggagt tttaggttat
    68461 cgaacatcaa aatctcaata tggagacaat gaaaaaacaa aaaaagagat taaagaagct
    68521 aaaaaagtgg cggaacagtt gaagaaagac ggctggagct ttgcttccca tacatggggt
    68581 cacttgaata tgactcaagc ttcactagaa gatgtaaaaa aagacaatga attgtggcag
    68641 caagaagttg cacctatcct aggaaaaaca aatattctga tttatccatt tggtgcagac
    68701 atcagcgact ggcagcctta ttcagcagaa aatcaaaaat ttgcttactt gaagagtcaa
    68761 ggtttcgata ttttctgtaa tgtcgatgct tctacaccag cttggggaca attaggtacg
    68821 gatttctatc gaaatgctcg gatcaatatc gatggcattc gttttcaatc tgatttagac
    68881 ggaaaaaatc ctattcttga tccattcatc aatgtccatg atgtctatga caaagaagca
    68941 cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa agccgaactg acattttatc
    69001 gtcagtttcg gcttttttat gctttatttc ttcttcggtg cgattccctt gatcaggact
    69061 tctgtccatt ctgctgtata tggaagcagt tttgcgtata tctccggctt agaaattcct
    69121 tctaccattg cttgaaaata gaggatcaag aactcatccg aatatttcaa atccactttt
    69181 ccttctttcc gccctcgatg gaaaagatca agcatgatac tgaaactttc ttgcgtatac
    69241 gcatccatcg cgtcagaaaa ttcttttttt tctttttttg tatagtatgt catcaaatcg
    69301 atataaaatt gtttgttcac tcgattgaga taacggattt tgttttcact catggcaatc
    69361 agtgtttctt caaatggaag attttccgca atcaacgctt tcgcttcttg ccctaaatca
    69421 ttcataaatg caaaaaatac ttcataaatc agcccttcct tgctgccaaa atatttaaaa
    69481 atcgtcgctt tacttacctg agcgttctca gctaccttat ccattgtcac tccagcggtt
    69541 cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt
    69601 gctctctttt caaaaccgtt catttgtaga gcctcccgta attttaaact tttataatat
    69661 atatagttca aatttattct gaggtttatt ataactatct attgagaaag tatcaagcta
    69721 gcaattcctt gtttatgaat tcattgacag aaatttataa aaagaatatt atgtactaaa
    69781 ataataaaaa aagttttata aaaaggtata aaaccttttt attaaactat ttaatcttgt
    69841 ttagtatata tttttagaaa aaggagtggg gtttttatgg cagaaatcgt caaagtcaca
    69901 catttgaaaa agagctttgg tcgtttcgaa gctttaaaag atgtctcttt caccgtacat
    69961 tcaggtgaag tcgttggatt tatcggacca aatggttctg gaaaatccac gacgattcgg
    70021 acacttttag ggattataaa aaaagacggc ggaacggcaa cgatttttgg gcaagatgta
    70081 tgggaagata gtttatctat ccatgaacgt ctttcttatg ttccaggcga tatctctttg
    70141 tggggaaatt tgactggagg agcgatcatc gatctcttca tgaagcttca tggaaaaggg
    70201 agtgctcaaa aaaaagatga actgattcaa cgtttctctc ttgatcctaa gaaaaaagca
    70261 aaaaactatt caaaaggaaa tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat
    70321 tcggatctct atttatttga tgagcctact tctggtttag atcctttgat ggaaacgatt
    70381 ttccaagaag aagttgaaaa attgaaagaa cagggcaaat ccatcctact ttcttctcat
    70441 attctcagtg aagtcgagcg actagcggat aaagtcgtga tcatacgcga aggaacactc
    70501 gatgaagaag gaacactcga tgaattgcgt catttgactc gttcgaccat cactatagaa
    70561 acagaagcag agaatagtca cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa
    70621 ggtcacctcg ctcaatttgc tgctgataac gaagctttcg atgtgatttt agctgaagcg
    70681 tccaaattag gtgtgaaaaa atttgaatct attccaccga ctcttgagga tttattcatg
    70741 agacattatg aaggatgagg aggaagcgaa atgactgata aattttcacg ctggtccacc
    70801 cttttttctc aaaaccttag ccgtgactgg aaaaagatcc tgatatggct agtcggtgtc
    70861 actgcttttt ctggtggttt tgtccctgcg tttgaagaat tgacttccgg acaaggatta
    70921 gctggattat acgaaacctt gcaaaacccg gcaatgattg caatggtcgg accaactcca
    70981 gcttcgaatg ctgctgagta tactttagga gcgatgtatg caaatgaaat gttgcttttt
    71041 tgcagtttgt ttgctgctgt gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa
    71101 gaagaacttg gtctgattga acttattcgt tcctttcgag tcggacgtca agcaaactct
    71161 ttagctgtca tcgctgaaag tattctgatc aatttgcttt tagcaatatc gatcagctgt
    71221 acgatgattt ttttccaagc ggattcgatc actgcagaag gtgcttggct ttttgggcta
    71281 tctgtaggta gtatggggat tttggggagt gccgttgcac tgtttgctgc acaactgatg
    71341 ccaagttcag ccagttcgaa tggtctgtct ttagcgtttt taggtggact ttaccttcta
    71401 cgagcaataa cagatgtagc tgattcctcc ctttcgatgt ggaatccttt aggctggtct
    71461 tatttgacct atccatttac aactaacaac tggattcctt tgctttattt gtttttgttc
    71521 agtctaatct tgataatcag ttcatttgtt ttagaggaac atcgagattt gaacaccggc
    71581 tatatccccg aacgtgaagg aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt
    71641 ttattattct atctaaataa agggatgaca atcagttggc ttatcacttt tggtttgatg
    71701 ggggcggcat acggctctat ctacgggaat atgcaacagt ttttagaaag taacgactta
    71761 atgaaacaga tgtttatcca atctggcgtc tcaattgaaa aaaactttac aagcacgatc
    71821 atggtagtct tgatttgtct agctgctatt ttaccaattg ctagcatcaa tcgtttattt
    71881 acagaagaaa cccacggacg tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa
    71941 ctttattgga ccaatatgtt actagctatt ctatttgcta tattggcaat cctgattagt
    72001 tctggtgcac tagggctttc tgctttggct tcgatgaagg atgcgaaaga catggatcta
    72061 ttgatgtttc ttaaagccgg gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt
    72121 agtggctttc tattcggatg gcttcctcga atcggtaaac ttgcttatat ctacttagct
    72181 tactcctttg cactcaatta ctttggcgga attttggatt taccagaatg gttttccaaa
    72241 acagctgtcc ctagctgggt tccacggatg ccaaacgatt cttttgatct ttccgtgttc
    72301 cttacgttat catgtatcag ccttgcactg ttcgtccttg gttctattgg ttataggcga
    72361 agagacttac tagaaggata ataaaaaagc atgtacaatt tcatgctagc ctccaaatct
    72421 gggtcaaaaa tccaacattt ggaggtcatt tttttattta caagtcttct aactgctctt
    72481 tatcaatgat ccagacttct cgattctttc tttttacata accattctcc tctaataatt
    72541 taaattttct agataaagtt tcgggggtcg taccgataaa tgcagcaagt tctttcattt
    72601 tcattggcag gaaaaagtga tccttccctg tcgctttagc taagtcaagc agatagacag
    72661 cgactcgttc ttctactttt tccatcatca aaaaagttgt ctgctgctgt acttttgtta
    72721 atttctcaac gttgattgct aaaagtttca gactaagggc gggataatgc agtaagagtt
    72781 ccgagaaatc tttttgtctc aatatgcaaa tttccgtttt ttctagtgct tcgccaaata
    72841 aagactcatt tggaacgcct agcaactggc tctctccctc atatccgccc ggctctatta
    72901 ctcgtaaaag ctgttccttt ccgctagcag ataattgata cactttcata ctgccggagg
    72961 ccacgatcac taattgaggt tctccattcg gcgcaagaat ctgttctcct ttttccacat
    73021 agtgatgttt gacaagtgcg tgtacttttt cttgatcttg ctgtactaag tgattgaaca
    73081 aaggaacaaa tgaaacacat aaatgatcat gactcataat ctctcctcct tctaaacagc
    73141 tatttctaat aaagaaacat aacttccctt attccaactg ataattttct tttatcatag
    73201 cataaacgtc aagcgaacca gataccagca aatagagtgg atttcttttt gtcccttgct
    73261 ttattttcct tcttttgaga ccttaagtaa ttgattccta tcaatttttt aaatatttac
    73321 tttttgaata atcaaaaaca aaccttcact cgcaagccga gagtaaggtt tgtttttcgc
    73381 attgcttatt tcgtgttgtc tattgaaatt tttccgcacc tagcttttga tatagtggtg
    73441 ctagttcttg taaggtttca gcggttattt gctcaacgtg ttcgatttct tgcggataga
    73501 agaatagttt acccatttga aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt
    73561 tctttgtatc ggacaaatag tcagacaaag ctaagtggtc gtcaagttca tgttctggtt
    73621 gcggccatat gtaataatcc ttgatatcga ctgttttgct ccactctttc agtatcgcaa
    73681 gcaactggtt atacgctgca atgctaccat atcgttcttc gcttttataa tgttggtaca
    73741 tcaaatagat ttggtattgt ttttgattca acaaaactgc tagacaggca ttctcgtctt
    73801 gacgagattc gcttcggtac gcgctccaaa aatgactgcg taaattccag ccattcgtcc
    73861 agctttcgat tttaggtttg ctcatgccgt acatatcagg caaaagtgca gctgtctgga
    73921 gctgaatctc tttccatttt tgccagtgtt ctttatattc tttcttgatt tgttcaatct
    73981 tatctgttgg atggctctct ttgagctgtt tgaatgcgta gattggtcga tcaaacaact
    74041 gataatctga ttttccaagt tccattgttt cgcctctttc cgttatcttt tgtattctac
    74101 ctgaattttg cgatatggac aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata
    74161 agaacagcca aacaaaaaac tgcgatgagc atcaccgcag ttttattgaa cttaaggttg
    74221 attgatcaca tctaaaagcg ttgaaaataa aatggcacta gaagcagctc ctgggtcgat
    74281 atgtccgatt gcacgctctc ctagatagga agctcgtcct tttttagcta ccaagacttt
    74341 ggtgtgttct tttgcctggt caatgatttc ttttgttaaa ttccctgcct tcaatgcctc
    74401 tgtgactggc agccaaacat ctaacatcgt tttatcgtca gcttctgcct ttcctcggct
    74461 ttgaataccc gctgttccct gctcgatcac ttcgcctaat ttttcttgag aatcaataga
    74521 atcgacctct tttgtcgctt tcatcatgtt catgaatgca gatccgtata acggaccaga
    74581 agccccaccg actttcgata tcatcgccat agagaatact ttgaatgtat cgctgattgt
    74641 ttcaggatga tcggcttgaa aagcttcttc ataagctgaa acccctcttc ccatgttatt
    74701 tccatggtct ccatcgccaa tcggggtatc tagatcactg agatattgtt tgttctgatt
    74761 gattgcctct gcaaattgcg tgagccattc ttggatttct tgaacggtta gtttcatatt
    74821 atcacccttt tatcttgaga ttttttacca tgaaatcgtc tgtacattgc tttctaaaga
    74881 atctttccat tgtgtctccg tcaagtcgat cagtgttaga gaaagacctt gcatatctag
    74941 tgaggtcata aaattaccga cttttcggaa aactacattg acgcctcttt cttccaacag
    75001 agctaaaaca tcattcatga acacgaactg ttccatcaaa ggcgtcccgc ccattccgtt
    75061 gactagtaca cctgcttctt ttggtaaatt cgagtaatca tctaaaatct tagtaacaag
    75121 ttcctttgcc agtactttag aaggctgcat cttttctcga cgatagcctg gctcaccgtg
    75181 gataccaaca ccatactcga tctcatcttc tcctagttcg aatcctggtt tccctacttc
    75241 tggtacagtt gctgctcgaa gcgctagacc gatcgtctta gttgcctgta cgaccttttc
    75301 tcccagttct ttcaactcag ctagggaagc accgtttctt gctgcatcgc ccacgatttt
    75361 gtgtacaagg acagtacctg caactccgcg ttttccagca gtatacgtac tattttcaac
    75421 ggcgatatca tcatctacga tcacactttc tacttcaata tcgtccatag cagctaattc
    75481 ctttgccata tcgaagttca atgcatcccc tgtataattc ttaacgatca gcaaaactcc
    75541 ttttccacta tcggcttcct taatagccgt ctgaatctga tctggagttg gagaagtaaa
    75601 cacatcacca agcaccgctg cacttaacat cccttcccct acaaacccgg catgggatgg
    75661 ttcatgtccg cttcctccac cagaaaccaa accaacttgt tccgctttct gattcttggc
    75721 tacaacacgg gaagattcta cacgatgaac aagttctgga tagcttttta ctaatccttc
    75781 cagcatttct tcgacaatat tgcttggatc attcatgatt tttttcaatt tattcccatc
    75841 ctttctttcc ggcattttgt gcttctgtta agatttgctg agtatcatct gttataccgg
    75901 cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc atccaccaga tgataacgtt
    75961 gctgctgctc ttcttccaac atgtcttttg ccagttccga gttcaatacc gcgctaccga
    76021 tatccgcaaa aacaaaataa ctagctgcat cgcttgattc attgactgct tctacgattt
    76081 tcatcgggtc tgatccgatc tcgccattta ttccgccgag agaaaaaatt tgcacctctt
    76141 cggatttttg catctgctca atcatttctt taataccatc tgttactttt tgactatgag
    76201 aaataagcaa aatacttttt ttcacaagaa tcttcctttc ttttacatag ttgctaacga
    76261 ttggtttcgc tttcattaat gaacaattca agtatataat aaatttctaa ttttgggaaa
    76321 atatgtgtag tgcttcttga agtttgttat actttaatta aaagagagga gtgatcgaat
    76381 gattacaaca acaacgaaca gtgtagaggg aaaagccgta gtagcttata aagggatcgt
    76441 ttttggtgag gtcattactg ggatccatgc atttaaagac ttggaggcgg ggttgcgaaa
    76501 tatctttggc ggccgctcaa aaagctatga aaacgaacta atgggtgcaa gagaagaagc
    76561 acttgccgaa atggccgaac gtgcaaaagc attaggagct gatgcgatca ttggcgtaaa
    76621 aatggactat gaagtattag gcagcgacaa tggcatgctg atggtcacat gtagtggaac
    76681 agctgtctcc acgagataag taggggacat ctgttttaat aactaaagag agatgggaca
    76741 tgtagtggca ctcaagaatt agatttttca gtctaacttc gaggtactct accttcccat
    76801 cctctctttt ttatttcttt ttgcggatga ttccgtaagc tgctgcgact aacacacccg
    76861 cccctgctaa ggaataactg acagaagctg ttttttcatt tgtttttggc aaagtctgac
    76921 ttgttgattt ggatggctct gtcgtttgct gaactgtttc tgtcgtgctt ccagaggtca
    76981 ctgatttttc tcctgccgaa tgcaaaatat cttttattcg ttggaaacta gctgctaagt
    77041 cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc ttcgctgatc tttccgtgga
    77101 ttgcttgact ggccgctgtg atatccgctt gagtttgact tagctttcca gaagctgttt
    77161 gagcaacctg atttaattgc tgattgaatt cttttgcttg ttggatcttc ccgtcaagat
    77221 ctgtttggac agcttccgct tttgctttcg cttcggcaag ctttttctca agttgtgcac
    77281 ggtcatttcc cactttattt ttaagatcag ctaaatcttg atcgattgct gcttggtctt
    77341 ctttcaattt gttttttgca gcttctaatg cttcttcgag ttcttttctt ttattttcca
    77401 agtcttgcaa atgatcgatt tggtcagaag aggtagaact atttattgat gtatcttctg
    77461 aagatgaaat cgtttctcct gaattatctg tcggtacatt ggaagtcatt tctgtttccg
    77521 aggcaccgtt ttcagcacca gaagagctta tggttgttga aggttctgtt tgagcaggtt
    77581 cagaagtttc cgcaaaagct gtctgtccca ataagaaaat actggaaaca gcaattggcc
    77641 agattaacgg cttcattctt tttttcatca cttttcccat ccttactact ttttaactaa
    77701 tttcttatta taagcaaaaa tgtttggaag aaccataaac acaacaggct gtttgtctaa
    77761 aaaacggtgc atcttttatt tttttgtacg ttccgggctt ttccatgtcc cgcttccata
    77821 acctaaaatc gttttgactg ctggaatgaa agtcgtcacg atcgttatcg cgttcactat
    77881 ccagaacaaa agcatatata gtggtgcgaa aagaaggtat ttcaattttc ttcgtcgatc
    77941 atctacaatc agcgaagcta gcaactggaa aaatcctgcg accatttcaa agcagatgaa
    78001 cacaaaactg aatgtaaaag taatataaat ctgttcgtag tttccttgat atacataatg
    78061 cccgattaat ccagcaaaaa gaactacgga aatacaaaag aatattgacc agacaatact
    78121 caatgtttga tcaatgaaga tgatcgttct tccaatatgt ttaaaaggat ggagcatcac
    78181 ttttttgaaa ttcgtcagcc agacttctgt tccacctttt gcccaacgtt ttctgtgtcg
    78241 atatagcatc ttcaacgtca caggtacttc catgaaaaag ataatctcag gagcaaaaac
    78301 agataaccag tcatttaact gatgatccca agcaatactg atgtcttctg tcgctcgatc
    78361 ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga tacatcgtat ttgctccact
    78421 gtacgcataa agaccaccga aaacagctga ctgtgttcgt ttaatgatgc ccacgatact
    78481 tgagaattca accgtttgag attttgcaat caatttggta cgattttgga catccatatt
    78541 tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg aaataattca catacctaat
    78601 cagtgcatct ggctccggta ccgtgtctgc atcattgctt aaaatcaatt tcccttttgc
    78661 aaaggcaagt ccgatattaa acgcgtgggc ttttccttta tttttttcga tccgaaccac
    78721 acgaagatta gcgtattttt tcatcagtct agctaatatt tctggtgttt ggtccgttga
    78781 accatcgtct gtcactaata cctcatagtt gtgataattc aatttcgtca tcaaatactc
    78841 gattgtatat tcgatcacaa tttcttcatt atgggctggc accatgatcg tgatgaacgg
    78901 ttcgacactc aagggaacat ctacccattc tttttgctta tgtttgtata aaaacacata
    78961 acaccagaca ccaataaacc atacaaatcc gcctaaaatc ggataagaaa cgagaaaaaa
    79021 atacataata gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat
    79081 ttcagttcca ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc
    79141 tcttcggaga cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc
    79201 gtatacactt cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattcaaaa
    79261 gatttattta cttttttgta tagatttcga ttgttccacc tagtcaatac aaatgaggta
    79321 atcaggaaac tgaaaaaaat caatacaaaa aagccgatta agaaatacag cattttcttt
    79381 tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa
    79441 aagacccata taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc
    79501 caaatcttta accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca
    79561 acttcttctc ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg
    79621 agtttaccat acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa
    79681 agaacaaaac atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct
    79741 ttttattttt tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct
    79801 ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat
    79861 ggatataaga ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg
    79921 cagttttcta atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct
    79981 tctgtttggg tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt
    80041 attttcaaca attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt
    80101 cggatcgata cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg
    80161 attgctccgc tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag
    80221 tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc
    80281 tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt
    80341 gccagggtat aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc
    80401 ttttcaacaa gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg
    80461 ggttgccaag cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc
    80521 tcatccaccc atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa
    80581 agtttccttg cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat
    80641 aattccaact gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt
    80701 gcttgtttca aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc
    80761 ctatcgatca atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta
    80821 gaaaggactg tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac
    80881 aaaggatctg attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc
    80941 tccgatacat tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct
    81001 gctgaaagct gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat
    81061 gatggtagga cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag
    81121 tcatcggatg tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata
    81181 gcagaggttt taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc
    81241 cgaatgattg tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc
    81301 tcatcttggc ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa
    81361 aacttgacca aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg
    81421 attttatttt gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg
    81481 gcaattggtg aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct
    81541 gtaccgatat aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca
    81601 aagtagggac ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt
    81661 ttgatattca tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg
    81721 ttatctaagt aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg
    81781 tttccttcta tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc
    81841 ttttgataca cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac
    81901 attctcctct ttttttaaag tggtggaacc gattagttta cctcttttaa gagtcgaatg
    81961 caagcttttt aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg
    82021 atataaagaa aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct
    82081 ttccgctaga tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc
    82141 taattcattt aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt
    82201 gcccttttcc aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc
    82261 tgtcggcaat tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc
    82321 atatcctagc atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc
    82381 taattccggt aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat
    82441 ccgtttatcc acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg
    82501 atggaagatc aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt
    82561 tcctgataca tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa
    82621 accggtggat gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg
    82681 atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg
    82741 tgaagcaaga gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc
    82801 ccccaccgca taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc
    82861 gcgtatgcct aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat
    82921 ggataaaata actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc
    82981 gtggtcttca aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa
    83041 ttctttttct ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg
    83101 ttcgatcacc atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat
    83161 gaatcctgca ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt
    83221 atcgatatct ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat
    83281 cgatggaaca actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg
    83341 tttttgaccc tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc
    83401 atgatttgga cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag
    83461 aagattttcc cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa
    83521 agaaacatac ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag
    83581 ccgtgtagca gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt
    83641 tccctcctac tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat
    83701 aaccagattt ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca
    83761 catagacggt cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt
    83821 tagctccttt tatatgcccg ccccggtatt ctgcaggtgt tctaacatca agtaacgctg
    83881 gattttccaa aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata
    83941 gcaaattcat tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa
    84001 caggagcttt attaatatgt caaccagaat gactcttcat ttttttcact actttttata
    84061 tttgcataaa ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta
    84121 tggagattga taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg
    84181 gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg
    84241 ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt
    84301 gctttgagaa acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca
    84361 agatgattat taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa
    84421 ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa
    84481 acgacgattg tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat
    84541 gcagaattct tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct
    84601 aatcatattt cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt
    84661 gatttagttg aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt
    84721 gcgacagaag tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg
    84781 aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa
    84841 acaaaaatag cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    84901 tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa
    84961 aaacgggaaa atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg
    85021 attgaacacc gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa
    85081 aattttgtga aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg
    85141 gcttttgaat accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga
    85201 gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc
    85261 agctgattga acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa
    85321 gaaataattt caaaaacaac ttctcctgtt tttggttcgg ttggcttatt atcgagaagt
    85381 agctactggg agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta
    85441 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
    85501 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
    85561 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
    85621 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
    85681 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
    85741 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
    85801 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
    85861 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
    85921 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
    85981 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
    86041 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagaca ctgatattat
    86101 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
    86161 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
    86221 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
    86281 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
    86341 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
    86401 tattcattga caaatatccg atcacctttt gcaaagagct gacgttcttt tttattctcc
    86461 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
    86521 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
    86581 atccacaaag cttggctttc tcctgtttct tccaacccgc aactgatatt taggacgaag
    86641 tgcttcccga tcgaaagcca tagcagccgg cacaaaatct tcatgatctc cggcgaattt
    86701 cttgatttct ccataatggg tcgtggcaat gatcaatgcc ccttgctcat acatagattc
    86761 catgattgcg atcgctagtg cagccccttc atttggctct gttccactgc caatctcatc
    86821 taaaagtacc aacgtattcc tttttatctt tttcaaaata gcagcgatgt tttgcatatg
    86881 accagaaaat gtactcaacg cattttctaa attttggtgg tcaccgacat cgacgaaaat
    86941 ctcatcaaag acagcaagct ctgtcccttc ttttgccgga acttgcatac cgaacatcgc
    87001 catcaaagtc aataaaccga ctgtcttcaa cacgactgtt ttgcctccgg catttgctcc
    87061 ggtgataatc aagcctcgat agtctttacc gatttcaaca tccaatggta ccgcatgctc
    87121 aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt tccgatttat tgattctagg
    87181 tgtgcttcca ttgatttctc ggctaaattt tgcccgagca aagatgatat ctaacaccgt
    87241 gatcgtttcg atcaataaat cgatcacttc ttcgttttct gcgatagcac cagtcaatgc
    87301 cgccaggact tgatattctt cactgatttc ttcagctttt aacaactgat agtgttcatt
    87361 caatttagaa atcacagttg gttcaataaa aaccgtcgtt cttttatttg attcatcgat
    87421 aatcgttcca tctattttat ttttatagct ggctttgata ggaatcgtat agtattcgcc
    87481 tttttgaaca atcattgctt cttggatcat ttctttgttt ttcggatgac gcaagaattt
    87541 cagtaatttt gactggattt ctttttcgat tagttgtaat tgtttgcgta ctttacgcaa
    87601 attacgtgac gcgtcgtcac tgactttatt attttttatt ttctggtcaa tcaactcttc
    87661 tacatttatc aaatcaggaa gatgtttgct ataagcaaac aacaagggtg tctggtattg
    87721 attcttatca aaaaactttg tgatcatccg actgcttcta agaaaatccg catattcgat
    87781 caaatcggtt ggctgcagaa tcaacccttt tttgacttgt tctgtcagtg catcgatcct
    87841 cggcaacccc ataaatggaa catgctgact gctttctaag atcaagcgtg cttctcttgt
    87901 ttcttcttga cgatccaaga cagtttgtag gtttgtcgag accgtcatat cgctgattcg
    87961 ttttttgcta tagtttccaa ttgctcttgc ttggatttcc tttttgatcc gatcaaattg
    88021 taattgacta atcatttcgt tgtccatatt ctctcctttt cttgaagaga tacctgtccc
    88081 aaaataaata agggcctaga gataaataaa aggatagaac acagcttaaa aaagtcatgt
    88141 cctatcctta ttagaataca aagatattgt tttcctgaca ggtaccgttt cataagcaca
    88201 ccaaataaac tttgtttatt ggctattcga cttactgatt agataccagc tacactcaca
    88261 aaacataccc cattcttcta ggtcttattt attttccact taactatagc aagacacatt
    88321 ttggttgtca aactttagta tgttttatag atttacacca ttaatgaata gacacctacc
    88381 gcgataattg ctccgatcat tggacctgcg attggtaccc atgaatatgc ccaatctgaa
    88441 gttcctttat tagctattgg caataccgca tgtgctaatc gaggaccaaa gtcccgagca
    88501 gggttgattg catatcctgt tggccctcct aatgaaagac cgatggctaa gatcaatcca
    88561 cctacagcga atacgttcgt tccaggtgca aattcgtttt gtccaaaagc tagcaaccca
    88621 agaactaata caaatgtacc aattaattct gtgatcacat ttgctggata attccgtata
    88681 gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat ctttcgttgc attccaatgt
    88741 ggcaaataag aaagccaaac aagaatggcc cctgcaaaag ctcctaaaac ttgcgcaatg
    88801 atgaaaggaa cgacaagatt ccaactaatt gcccctgtca tcgccatagc tagagagact
    88861 gcaggattca aatgagctgg gcccatgaat ccagaaatat agacagccat tgttacagcg
    88921 agtccccaac caaatgcgat gacaatccag ccagaagcct gtgctttact tttgtttaaa
    88981 ttcacagccg cacacacacc atctccaagc aaaactagga tcagtgtccc aataaactcc
    89041 cccatgattt gagtcatatc actgttcatt tatttctccc cctttagttc ccgcagatct
    89101 gattctgcaa tcaatgcttc taaacgttta gtctgttgcg ccttctcctc ttctgtccat
    89161 tcaaaatatc cagcaattgc atcgatcaca ggttgcttga tctcatcaag ctgatctcgt
    89221 tcaaacaaca gatgatttgt acgacgaatg agataatcgc ctggggccaa aaccatttct
    89281 tcttctaaac cataacgtaa ccgagctgac tcagcgagac tcaaaccagg ataaggtgcc
    89341 atttcttttg ctaattcaaa gatacgtcgg gcattagtcc cgtaaaaatc agcgatataa
    89401 gtagcatctt cttcctctaa accggctgct actccttctt ttgctaattc tgtcactgtt
    89461 tcttctaatt tagttggatc gaagtttcca cctgaaatct gatatttttt agagtcgatt
    89521 tctttcgtct ctattccgta ttcttctttt aataattggc gtatcaatcg caaagctcct
    89581 tctgccatct tacggtaatc ggtgattttc ccaccagata aagtcagcaa accatctggt
    89641 tctctttcta aagaactacc tctggaaatc gtagaaggtg ctttttcatc acggctgttt
    89701 tccaaatggt tcaacacgtc ttctacttca gcacgagaaa ctttgttttc cttatattca
    89761 cttactgtat cgacaacttt attgaagctc ttgtctgaaa tcgatccatt atctccacca
    89821 ttgtaatcag aaccagaatt accaatcaac agtggacgaa gtcctgccca gcttgcttcg
    89881 atatcattca atgtgatatt tgcttctgga tagcgatgat tgatcacatc caatagataa
    89941 tctacatctt cttgtgtgac tttcggatcc gtaaaatctc cttggtaatc cgtatctgtc
    90001 gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa ccatccgccc atcttgcttt
    90061 cctgtatcga agtatgtggg ttgcggtaca ggcagttttt tcgcatctac aactaaatgg
    90121 atccctttag ttggacgcat tttaggagag actggacgcg taaaattcaa gtttcgtact
    90181 ttatctaccc aaggaccact cgtattgatc actaattttg ctttgatttc gatcacttca
    90241 tctgtcagca ggtcacgagc tttgacgcca acgatttgat cgccatcata taaaaagcca
    90301 accgctttca ttttacttac tagataagcc ccatcttcgg cagccttttt gatattatca
    90361 atcactaaac gggcatcatt gttgcggaaa tctagataga cacccgcgcc ttttagccct
    90421 tcttttttca aaaacggttc tctttccaat acttcttctg gtgtcagcgt ataattctca
    90481 tatttggttc ctgtcacatt tgccagtttg tcataaaggt ccattgctac tttgaccgag
    90541 aacatattga aggttgttgc tccttcgccc tcatagattg gcaaaagcat tggatctggt
    90601 tttgggatgt gtggggcaat actttgtacg actgcacgtt caccaactgt gtcagctact
    90661 acttccacat caaatgtctt cagataacga ataccgccat gaacaagttt ggtcgagcga
    90721 gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc ctgttttgat tcctgatgct
    90781 gctgactgga ctgccacacc ggcaccagtg attccgccac cgatgatcaa cagatccaat
    90841 tcttcttgct gcattttttg aatgctatct tgtcttgtct tgtttgaaaa catatggatt
    90901 tcctccttat tcaaatgctt gggtagcttt tactgctttt ttccaaccgt tgtaaagttt
    90961 ttctcggcgc tcttcctcca tttgaacttc aaatagcttg ccagcttcat aaaattcgcg
    91021 gatctcgtct gtatctttcc aataaccgac agctaatcct gctaaaaatg ctgcccctag
    91081 cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca ttcaagatat ctgtttggaa
    91141 ttgcatcaga tattcattgt ttgctgcacc gccatctact ttcaatacag gaatcttgat
    91201 tccagtgtca tcttgcatcg tatcgatgat atctctcact tgataagcaa tcgactgtaa
    91261 agtagcttta atgatatctt cttttgtcgt accacgtgtc aagccaaaca tagcccctcg
    91321 tgctgcctga tcccaataag gagcacctag cccaacgaat gcaggaacaa cataaacctc
    91381 atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg ctagcttttt gcaacatttg
    91441 aagtccatct ctcagccatt gaatcgaaga accagccaca aaaatacttc cttctaaagc
    91501 atagtaaacc tttccattta tgccgtaacc aattgtcgtc aataaattat ttttggataa
    91561 ttgcggtttt tcaccggtat tcatgacaat aaatgaacct gtaccataag tattcttgac
    91621 catgcctggt tcgaatgcca tttgtccgaa taatgcggct tgctggtctc ctgccatccc
    91681 tgaaattggt gcttcgcttc cataaaaatg ataaccttgc gtataaccat aaacttctga
    91741 gttagaagtg acttttggta acatcactcg aggaatattt agtaaatcta aaatctcctg
    91801 atcccaatct agctgatgga tattgaacaa catcgtccga ctggcattcg agtaatcagt
    91861 aacatgtgct tgtccatcgg tcagtttcca tactaaccaa gaatcgatcg ttccaaacat
    91921 caattcgcct ttttcagctc gttcttgcgc gccttctaca tgatcaagaa tccagcgtat
    91981 cttcgttgct gagaaatagg catcgatcac taaccctgtt ttttcgtgga aaaattcttg
    92041 atagccttca ttttttaagt tatctgcaat tccagctgac tgtctggatt gccaaacaac
    92101 tgcatgatag attggacgtc ctgtatcttt ttcccatatt actgttgttt cacgctggtt
    92161 cgttatgcca atcccagcaa tctgatttgg tttgatccct gattcgatga acgctccggc
    92221 aataactgac tgtacagaat tccagatttc atttgcatca tgttccaccc aactttcttg
    92281 cgggaaatat tgcgtgaact ctttttgaga actgccgatg tgtcttcctt tcttgtcaaa
    92341 gataattgct cgtgagctag ttgtaccttg gtcaatcgcc atgatatacg ttgattctgt
    92401 cattgtgatt ccctccatta tatgcaaacg ctttctttaa ctactcttat catactactg
    92461 ttttaaaaaa agaaactgtc aaaattttat tctttttttc tttttttgac agttttttat
    92521 tatgaagcta ttttttaatc aattcttttc ttttttcagc gtctctattc ttcttttcaa
    92581 tcgaataatg tcgtaaggtg acgcaaattc tgacagataa tattcacact gttttttgtt
    92641 tctctggcaa aatgtgatca ccaaatcata ttctttacct tcttcataac ttccaatctc
    92701 aataccgccg attcccctca gttctctgat cagatattgt tgaaaagcaa tacgataaat
    92761 cggcagactt tctaaatcga ttccaatcac tactggtttt gcaatataaa aatcgatcat
    92821 caaaagaaca ttggcatact ggatcatcag gtaactaagc tcactatctt cagctctttt
    92881 cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc agtaactgtg ttaaggaatg
    92941 cccgagcatt ttctgctgct gttccaaaag ccgatcacgc tcatagactt cgatctttcc
    93001 aacaaagaaa aaccattcat tgttgatctg ggaaatttgg tagcctactg ccttttcttc
    93061 tttgattttc aaacgacgag gacgataatg ccataacatc gtttcccgaa tgtaagtatc
    93121 taaaacagcg gttggtaact ttttgctccg tgtcaaatca tactgataaa atatttcttc
    93181 gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc attgtttcgt atacattcag
    93241 ttctgctgct gttcgactaa gatgcatcac gatgattgga tcgattgctt gataaagccg
    93301 gtccgactga tagagtaatt tcttttcttt caaagttgcg tacgtacttt tttcattcaa
    93361 tagtcttttt ttcgtaatcc cgagccagca agcaatctgt gcttctgctg atgccgtaaa
    93421 cgttgtttct aatacacggt ccaatcctag aatgaacggt cttttttctg gtgtattttg
    93481 caaaaaaagc ggtcgctgat cttccgggat atattggaat agttcataat aaaaatatcg
    93541 tatctgtagc tcttctccgt ataattgacc gtttttgatt tgtaatccaa attcctctaa
    93601 gagctggttc aattctttga tctttcgaaa aacagaagac tcactgatat taaacgctgt
    93661 cgctaatcga acgatggata cttgctgatg ttctaataaa tagacgagca tcttaaactt
    93721 caagctttcc tgcaataaaa acaatagaat tttttccaaa cttacatctt cgtccaacac
    93781 aagcagcgcc tgatactcat ttcgctgtac ttctactttt ttttccatca ttcggccaat
    93841 caattcaata tcttccaaat attgatcaaa cgaggctttt gacagttgga catgctctct
    93901 caaaccattg atagaggctg ttcccccttg cagaacaagg tttcgcaaaa tcaggatctg
    93961 taactgctct tttctttcta ataatgcttc aatcttcata ggcagtattc gccacctttg
    94021 accacaccat atctggcaca cggtattctg gctggaaaaa tagctcatgt tcttctaact
    94081 gttcaaacgt ccatccttct tctactgcaa ttgtcagttg gtcaagaagg tggaacatct
    94141 cttttttgct ttggagctgt gctccaagaa tctttttttc agaatcaaat agaaatttga
    94201 gtttaacttt gctttgattt attgctgtaa gcttgtagtc tacgtatcct gtttcaattt
    94261 tttggggata aaaaatgctc tcttcttctg tcaaacctac gcttcctaag aaatagccaa
    94321 accactgatt gccgactgga cgataggttc cctgatcctt tgtctgtgag ccactaattg
    94381 tcttgctgac atttcgtgct gtacggacag cgttatttac tagagaaaca tacatttttt
    94441 catttgttgg acgaaaagtt acttggatcg catcgccaat cgcataaacg tctttttgag
    94501 acgtttgcaa gtagtcatct acccagatcg ttccatcatc attcaatgtt aattgttctt
    94561 cccacatctg attgtcggga cgagaatgat tagccagcaa aacaagatca tagttctcct
    94621 ttcttttttc cgtctctaca atagcggttt cattttcggt caaagacaca acttgttctt
    94681 ctaaaaaaag tgaaagccct tgatttttca tttcctttac taacggttct gtcatttcag
    94741 gatctagata acgaaaaaga attgactttc ggctttcgta aagatggatt tgtttttttc
    94801 ttgatgccaa cccttcagct atttcgattc ctgcttgtcc tgcaccaata attgcaattt
    94861 tctttgcatt catgatcttt tgctgaagag ccgttacgtt ttgtatcttt ttaactgggt
    94921 gaataagcgt tgattctgca gaaatatttc gaaacttttg atttgaacca gtcgctagaa
    94981 tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac gacttttttt tctttcaatc
    95041 ctattactgt cttttctgtg taaacatgga ttcgatagcg gtcagtcagt tcttctttcg
    95101 tgatccaaga aagttgtttt tcactggtaa cctttccttt taatatcaaa ttgaacccgc
    95161 tgggaatcca tcctaacttc gattgctttt caatcaaata tatttcactt gttggcatca
    95221 attggcgaag ataaatagcc gcatgaatcc ctgcaaacga cccgccgaca attacaatgt
    95281 tcatctgcat tcctctttct tataaggtgt tctactgcta acttctcaat cagaaaacac
    95341 agtattcata actcgctttc aaccttatga tacatagcga tgcagcagaa tcaaccctct
    95401 ccatcatacc gtgttctagg tctgcttctc aataatgtac tagtggacct aattgaagag
    95461 tatcatttct gaagagatgt ttcaaaaaat gtactcttat aaaagaaaaa aactcgcctt
    95521 ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa aagacgagtt ttagcgtgtg
    95581 taatgtaata gttagaatag acacatcatt tatttttaat tctttaatgc ttcaacaact
    95641 gcttcgccta attgatgagc agattgagga ttttgccctg taagaagccg cccatcaatt
    95701 tgaaccactt ctgaaaaagc tgctccagac tgatagtttg ctcctttttc tttcaaagca
    95761 tcttctgcta agaatggtac ttcattcgtt gtaccatttg cttcctcttc ttcattactg
    95821 aacccagtca ctgtacgtcc agctaaaata gattttccag actcatctgt aactggcaac
    95881 aatcctacaa caccgtgaca aacagctgag accagccctt catttcgata gatttgtgaa
    95941 gcgactttcc caagatctat tgcttctgga aaatcccaca ttgttccgtg gccaccagca
    96001 taataaaccg ctgcataatc atttgcatcc acatcatcag gacgtaaggt atttcccaag
    96061 gcccgatttc tgaaatcttc atcttcataa taggcccaat cgatttcatc catattcaaa
    96121 acactttgcg gttctaaagg tacatatccg cctttaggac tgacaaaatc tgcatcgatc
    96181 ccatttttta ctaatacatc ataaaaatga gtcagttcac ttaaccacaa acctgtagga
    96241 cgctcgatct tttcgtactt ttctacattt gttaatacga tcaatacttt cattttccca
    96301 tcctctttct ataaaaattg cgttattgat aacgtgcttt caagaaatta tataatgggc
    96361 ttggcaaata aagctcttta tatgcttttt caaaaggttt cgttacaaat tcatcctcac
    96421 ggttttccaa taatttctct gcccagtcag gatcgacgat catttgctgt ccaattgctg
    96481 caagttctgc atatttcaat acctgctcta catcttcgcg attgcggatc ccaccgacac
    96541 caactaaagg gatctttcct tgaatcgtgt tatggaccac ttcaagaatc gatttatcgt
    96601 tgtatttatc agaacgagct attcgatcat acatatttaa tgaaacatgc agataatcta
    96661 gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc ttcaaaacgg atacctggtg
    96721 tttcatattc ttctggtgag aaacgatacc ctacgatgaa tggtttttta gcatattgtt
    96781 caaccgctga gaaaaccgtt gttaataatt cttcaataaa acgataacga ttttccaagc
    96841 taccgcccca ttgatcctct cgtctattcg aatgcggaga gaaaaattgt tggatcaaat
    96901 acgtatttgc tccatgcaac tcgattccat caaatcctga ttcaatcgct ctacgtgtgg
    96961 ctttgccaaa tgcttggatt gtttcaacga tttcttcttc actcattgct cgtggtgttt
    97021 ccgcatcagg acgttttgct gcaactgcac ttgcagatac aggctgttct ccagataaag
    97081 ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat aatcgctttt gcgccttctc
    97141 cttggattgc tttcgctagc tcacttaatc gaggaatcat ttcatcatga gcgatgctga
    97201 gttcacctgg ccagcctttt ccgatatctt ggacatttgc agcaccagta ataacagcac
    97261 caagaccttt agaacgttgt ttataataag tgatctcatc ttcggtgatc actccattga
    97321 aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg gttcttcaat tgaactcctg
    97381 aaggtaaagt aattgattga tcaaattgtg acattgtcat tcctcccata attcgctctg
    97441 attaacctat cgagaatcat ctatcattaa accactcgat tagttgtaca tgcaactaat
    97501 ttgcttaaaa aattttattt tttttgtaac ttatctgttg catcattcat tgtctgggct
    97561 aattctttac ttgcttcatt tcccaaaact gctttgtatg actgatgaag ttcttcccag
    97621 ctaccatcga cttccttagc aaattggcgt ccttgttctg taagataaac taatgacaag
    97681 cgaccttcgc taactgtttt gacgtatcct ttatattcta atttttcaac aaaacgagca
    97741 atcgttgatg gggcggaata gagcatttgt gcaatatctt tttgtggtat tccatctttt
    97801 tcttttaata tcaataaaat aaaaccataa gttggtgcca gacctgtcct tctaaacgct
    97861 tcatcagcta atttattcaa catgcgatct agctttttca cgctaaagaa aagacattca
    97921 tctaacgctt tttcaattgg cttttccatt tcatcccctc cattggcgta catatcatag
    97981 tatgattttt attttttgtc aaaaaacacg gaataatgtc cgcctcctct gtctagattt
    98041 gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt tttcgacaaa actccttctg
    98101 gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt ttgagtatga agaaaaattt
    98161 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaaa aactggcttt
    98221 tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct gcatcaagta ataagaacag
    98281 ccaaacaaaa atagttcctc atattttgtc gttttttgag ctaatgccga tggtcagctg
    98341 attgtacacc gtttattgag aggtcgtgag cctgccgttg aactccgagt cacaaggagc
    98401 aatttcaaaa aacagcttct catgttttat gactatggtg acttgtgatc gaggagttgg
    98461 caggagaata ctgtttattc gtaaagaagg ggctgtgaca agacttttgt cacagtccct
    98521 tatttccttt tattatagga tttcgccatt tgattggatg acttgtctgt accaatcaaa
    98581 tgattttttc tttaaccgtt tctttgtgcc attcccttcg tcatcttgat caacatagat
    98641 atatccataa cgttttgaca tttgtgaagt agaagcgctg acgatatcaa tgcagcccca
    98701 cgacgtataa ccaaataaat ctactccatc tttgatagct tccttcatct gctcaatatg
    98761 ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt ccatcttctt ctactttgtc
    98821 tatagctccg agtccgtttt cagctatgaa cagtggtttt tgatacctat catataattg
    98881 attcaaagca attctcaaac ccttaggatc aatctgccag ccccattcac ttgatggaag
    98941 atacggattt ttaattcctc cagttattaa gtttccttgg accccttccc aattttctgg
    99001 atgagcactg actgcactag acatgtagta gctaaaagaa acaaaatcaa cagggtaggc
    99061 tgctaagatt tcttcatcac ctacttctat ttttaagttg acaccttttt cttcaaacat
    99121 tcgttgcgta tacgctggat aatatcctct tgcttgaaca tcactaaaga acaaagaagt
    99181 tctttgctgc tcaattgttt tttgaatatc atccggatca caagaatacg gaacaagaag
    99241 ctgatagctg atcatacaac cgacttgtga acctttgata atttcatgac aagcttttgt
    99301 agctaaagca cttgctataa actgatggtg tgcaccttgg tatttcgctt gttccaaatg
    99361 gggatggttt tcttcaatta ctcctacgct aacaaaaggg tgatgtttta cacagttgat
    99421 ctcattgaaa gtcatccaat attttacttt atttttatag cgtttgaata ctgtttcagc
    99481 gaaacgaacg taaaactgaa taagctttcg attaggccat ccaccatatt ctgtaaccaa
    99541 atataacggc atttcataat gggaaagaga aacgactggc tcaatcccat attttagaca
    99601 ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca ttgggtgttt cttcatcacc
    99661 atgaggaaaa attcttgtcc atgcgataga catccgatag catttaaaac ccatttcagc
    99721 aaataaacgg atgtcttctt taaagtgatg atagaaatca tttccgtgtc ttttaggata
    99781 ataatacttg ctgttgggat cctgcgccgc ttttacttgc tgatgcgtca tcatatgcca
    99841 ttgatctgtc catttttctt taggtaaatc gctgtcgaat gtatacacat cagacacaga
    99901 taatcctttt tcatctacat cccatgcacc ttcacattga ttggccgctg tcgctccgcc
    99961 ccataaaaat ccttctggaa attttttcat atcgatcccc ctaaacttgc tagttttctt
   100021 gtttgattat ttttcttatg actagtaatg atatttttga atctactact atttgtaaca
   100081 ataaccattg tggtcggatc atatccctct ttgattaatt cttgcaaatc tacttccatc
   100141 aataaatcgc ctttacgtac tttttttcca tcagatactg cactttgaaa gtatttgcca
   100201 tttaaattca ctgtatcaat accgatatgg atcagtatct ctgctccttc ttcactgact
   100261 accccaattg catgtttcgt tggaaaaact gttgaaactt ccccgtttac agaactgtag
   100321 atttttccgt ccgttggaat gatcgcaaac ccttctccta aagatccact agcaaatgtt
   100381 ggatcgttta cttcagataa atcaattatt tcacctgctg caggagtact taacactata
   100441 tcctttgagg attcttcttc gatttcatcc ttgtagccga aaagaaaagt tagcatacag
   100501 gaactgatca tcgctaagaa ataactagca actagccaaa caaatccttc accaatataa
   100561 atcgggaaag acaaaatact tcttgtagca actgccaaag caccggctcc tgcggcacca
   100621 gctactccac caaatatagc tccacatata caagcaatgt aaaatggtcg tttatattta
   100681 agagtcacac cataaacagc aggttcagtg attccaaata atgctgtgat aaatgcagat
   100741 aaagataaac tttttacttt cttatttttc gttttcaaaa ataccccaag tactgctcct
   100801 gcttgcgcag ctacggcagt catacatgct ggacctagcg tatcacgacc gtagaaagaa
   100861 agattgttgg tcattactgg tacaagtccc caatgtaacc caaaaatcac taacacttgc
   100921 caaaatccgc cgataacagc accagataaa acagggctaa agttataaac agctaaatat
   100981 ccagctgcaa tcagctgact tgcataatct gcaattggac ctacaactaa taaaataatt
   101041 gggaccatca cgatgataca aatgaatggt gtcaataaat ttcgaatact ttcgtgcaag
   101101 cttttgcgaa tcttaggctc aatataagaa agaacccata cagctaaaat tgctggaata
   101161 accgtacttg tataccttgc tagaatcacg ggcatcccta gaaatcgcaa ggttatgcta
   101221 tcactatatg cactgacaat cgtaggataa acgagagctg ctgcaataac catagcgatg
   101281 aaacgatctg tattgaactt ttttgcagca gtatatgcca aaaatatagg caagaaatag
   101341 aaaaatgcat cagctgctgc attcaaaatg atataagttc ctgattcagt agttaataaa
   101401 ttcaaactcg tacataaggc gagtactcct ttgatcaaac cacttcctgc caagacgcct
   101461 aaagtcgggg caaaaacagc ggcgatcatg tctataaaag agtttaaaat gccttttttc
   101521 tcagttcctc tctttccact agatgcatca tcttctagtt tcatttgttt ggataatacg
   101581 tcatacactt catttactgc atttcctact acgacttgaa acattcctcc agcttcgact
   101641 acagtgatca caccttctag ttgctctacc ttctccttat ctgccttctt attatctttt
   101701 agggtaaagc gtaaccttgt tgcacaatgg taaacattgc tgacattttc ttcgcctccg
   101761 acaaatcgga caatatcttt cgctaattgt gaatagtcca tttttacttc ccccttgtta
   101821 attaatgccg tagcaatcct tcttacaaga taagtatagt tcttagaaaa cggtttctcc
   101881 attcaattag taccccttct aatagcgaac gaaactgtac aatagtgtgt atggtttact
   101941 tagccgagca aatcactcac tgcttgaaag aaaacaagta tatcgtttct cttatagtca
   102001 aaggagacaa aaaaaacacg cttgatcttc ctttgctgat gtataagtgg tatctcaagc
   102061 tgatactctg tgatttttgt ttctacttgg cttttgataa gacagcacaa caaagtctcg
   102121 gcatttaacg gaagcataac taggtcagaa ctaggagaga ttagttgctg aatggaatct
   102181 atctttttat ggatttttat ttgattttct tctttgtagt aatgatcaag cagttttgtt
   102241 ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa tctgataaat ctcccaagat
   102301 tcaaactcat ctagaacact aggtagataa atcaaatcat cccactcaaa aataagctct
   102361 tgctcagtag tcaaaattgc atcatattcc gttattttgt ttgattgatt tttgaaaaga
   102421 tacactggtt ctatccggca ttctttgatc cacatgttca tactttgttg aatctgtttt
   102481 tttagtgtat tcaaaatact aattggctgg ttactaacaa tcagtatctc cccttgtttt
   102541 cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat aagcgctttc agctaaattg
   102601 ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta aataaatttc taaaggataa
   102661 cgggagatga tcttttgtac aaaatgattc atggtattat gtccggcatc catcctcgtt
   102721 ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat ggaacaataa cgaaacagga
   102781 agatctaaaa aacgaataat cgtttgctca aatgctctta gattcttctc tatttctttg
   102841 tttttttcat cattaatcat caaataattt aactctaata gtcgattttc aattgcttgt
   102901 ttttcttgat tggaaaaaga atagggtagt tctgatgata acttttttgt gagggtctct
   102961 accatcggaa taaataatgt atacctgttt gttttttcta aaagttcttc tcttcgaaga
   103021 atggtcaaaa cgagataccg agaaaatcgt atgaagtcct ctcctgaaac aatataatta
   103081 tatgatataa gaacctcttt taatgtcttt tggacagtcg gcatgatatt ggaaaaaatt
   103141 gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg tttctgttcc aacaagagat
   103201 aagaaaattc tttttgaatt ttcttcagca atgattttta gtccttttgt cgacgaaact
   103261 tccagttcta atttaggatt gcgctcgatc caacgcaaaa tcgtttttat ttgtaatgat
   103321 acggctgttt ttgaaagaaa gaaaatgtct gctatctttt gcatactgac ataccctttt
   103381 tgaaacaata agtacgtcac aatagttgaa gctcggaaat ccatgctgga atagatttta
   103441 tgtttcgtca cctcttgtat gatttgctgt ttagtttcag attcaattga ttctaaaaaa
   103501 taacctgaac gtttatttga ctggataatg attttcttct cgtagatagc aagcatattt
   103561 ttgatttcag tttggatcgt tttcttgttg gtacctaaag cttcagctaa ttcttcgctt
   103621 gttatccaat gattcttatt tcttaatact acaagaattt ggagttgtcg tttatttaag
   103681 tccatttgct tacacctcct ctttgttata aacatacagg aaaaacctct caaatgacaa
   103741 tagtcacttg cagaggttta aaaatcattt gaagaaagat ctatattcaa ggtttttacc
   103801 attagactgg ataatttgtt tataatattc aaaactatct tttttcaatc tcctaaagct
   103861 tccgctccca tcattttgtc tatccacata aataaagcca tatcgcttcc tcatttcccc
   103921 tgttcctgcc gatacgatat cgattggtcc ccaccataag taaccaatga tttggcaacc
   103981 atcttttatt gcctcagcaa tttcctgaag atgtattttt agatactcac aacgttttgg
   104041 atcgtggatt cgattcaatt catctggtgc ttcatctagt ccaatgccat tttcaactat
   104101 gaataatggt aattcataac ggtctgtcaa ttcattcatg acataacgaa accctagagg
   104161 atcaattggc caactccaag gttcgggaga tttttccttc aaaaagggat tatcgaaacc
   104221 atttgctccg ccagtatcta cgcgcatttc tgcttctttt ttatagacag cgctacgata
   104281 ataactaaat gcaatataat ctgccgtgtt agcttgcaag cactgttcgt ctccatctaa
   104341 cattactggt gcacagtctt cttccttcca tgtcctgtaa atataagctg gataatgccc
   104401 tcggcacatg acatcggaga aaaaccatga attgcgttta aattgcagtg aaccaaatac
   104461 attctctgga tcacagtcat atgcataagt cgctaatgca gaaaagctac acataatacc
   104521 catcttagca tttggatcga ttttctttaa taattgagta gcacgggcgt tcgctagaaa
   104581 catatgatga caggcttgat aaatcacttt ttttgttagt gtagcaatgg gttcaagttt
   104641 atctctagca gggaatgtaa ccaatccagc agctgcataa ggcatacgaa aagcattatt
   104701 gatctcatta aaggtcatcc aatatttcac tttcccttgg tagcgttcaa agacggtctg
   104761 cgcataaaat tcaaaaaagt cgattaattt tcgattttgc caccccccat actctcctac
   104821 caatgctaag ggggtttcat aatgactaag agtaattact ggctcgatat catatcgatt
   104881 taatgtattg attagtctgt cataaaatac gagaccagcc tcatttggct tctgttcgtc
   104941 tcctcttgga aatattcgtg cccatgagat gcttgttcga aaagctttta aacctaactc
   105001 cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa tcaatcgctt catgagaaag
   105061 ataatggagt gattcgtcta aagcaatctc ccaaatcttt ttttcttcat tccactgaat
   105121 actaggatgc tggttcatga tcccgataag cgtatctgtc acattaggta atttgccgtc
   105181 ttccaaccat gcaccttcac attgattagc agctactgct ccgccccata aaaaattttc
   105241 tggaaatatt tcctgatcca taactatctc tcctcgctat caacaatttc ttacttgaag
   105301 tatagctcga ttcatcaaaa caagatattc taactatttc ggcagaagag aagaaaaaaa
   105361 ttgaatgcta ctcttcattt ttaatattta tatataacaa atacataaga cgatccccga
   105421 aagaaaacac tcgttaacca tctaagcaaa cgatttattt tacttccaac acaaatgctt
   105481 cataaggtcg taaatcaact ttgccgtctg gtaactgttt cagaggataa tttgacacaa
   105541 taacccttaa agcttcacgt ggaatagtga acgtctgctt ttcactggac atattacaaa
   105601 taacgagcca ttgctggtct tcatatatgc gagaataggc aaagacatgc tcaggcgtct
   105661 tttccactaa ttcatagtct ccccagacga tgatgtcatg ttttctacgc aaatcgatca
   105721 atttcttgta catataaaaa acagaatctt gatctgctaa cgcttcttct gcattgattt
   105781 ctttataatt tggattgact cgaagccatg gaattccagt agtaaaccca gcatgttcac
   105841 gggcgttcca ttgcataggc gtcctagcat tgtctcttcc tttcgccttg attgattcca
   105901 agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt atacatattg attgtctcaa
   105961 tatcttttgc ttccgcgata tcatcgattg ggcaattcgt catgcctaac tcttctcctt
   106021 gatagatata aggggtgcct ttcatcatat ggagcaagat agcaaacatc ttggcacttt
   106081 cttttcgata cacatcgtca tttccccaac gagaaacaat tcgcggaaga tcgtgattat
   106141 tccaaaataa actattccat ccttcatcac ctaaagatgt ttgccatttg gataatactt
   106201 ttttcagttc agagatactc aagggtttca aatcccattt gtctttgcct tcttgctgat
   106261 ctaatcctat gtgttcgaat tggaagacca tggataattc atttctttta ggattggaat
   106321 aaagtttagc gatttcggta gttgctcccc atgtttcgcc aacagtcatc agatttttgt
   106381 caccaaaagt tgcttgattc atttcctgaa gatactcatg cagtttaggt ccattacttg
   106441 tgatttcttt atccggttct ttgccaatca tatcgatcac gtccatacga aagcccccaa
   106501 tacctttatc gatccagaaa ttcatcattt gataaatctc ttggcgaacc tgtttatttt
   106561 tccaattcaa atcaggttga cgcttgctat acaagtgcag aaaatattgc cccgtttttt
   106621 catccaactg ccaagcagaa ccactgaata ttgagcgcaa tctattaggc tcttttccat
   106681 tgaccggatc acgccaaata taatagtccc gataaggatt ttctttattt ttacgggcct
   106741 caatgaacca cggatgttca tcagaagtat gattcaccac cagatccatg atgatacgga
   106801 tatttctttt ctttgcttct tcaatcagtt cttccatttc ttccatcgta ccgaattctg
   106861 ccatgattgc ttgataatta ctgatatcat acccattgtc atcattcgga gactcgtata
   106921 ctggagaaag ccaaattgca tcaataccta aatacgccag ataatccaac ctggaaataa
   106981 tcccccgcaa atctccaata ccatcttgat ttgaatcttg aaaacttctt ggatagattt
   107041 gataaacaac tgaatggtgc caccattttt gttccatgat tttcctccta taaatggatg
   107101 tttctatttt ttgcttaatc atttatttta gtctctctca ctaaaaattg taacgctttc
   107161 aaatagctat tgcaccttgt tatcggtaac agaataaaac aaaaaaacag ctgaggaaat
   107221 tccctcaact gttttttgaa aggattgatt tatgcaggga agaatgctga tttcacaact
   107281 tctttataaa cggtgttcac tcagctgtcc cttggtatta gctcaaaaaa cggcaaaata
   107341 taagaaacta ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac
   107401 ctctttattt cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca
   107461 tgattggaga tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa
   107521 aacctaatgc aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga
   107581 atgaagggat cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca
   107641 atactcctac cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg
   107701 gagaaaagaa caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac
   107761 gtcctccaac ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa
   107821 ataaggaacc cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca
   107881 tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat
   107941 caaagaaagt cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt
   108001 cccctacatg ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa
   108061 tttcagaagg cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgccatcc
   108121 cgatgaagat gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa
   108181 aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat
   108241 tcgaaacgat gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc
   108301 cagaaatcgc atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga
   108361 agattgtgat caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc
   108421 atggaatccc cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag
   108481 gtgccgttcc aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg
   108541 ctaatgcagt cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg
   108601 gatttacaaa taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac
   108661 gttttaccgt ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct
   108721 tccatccccc taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata
   108781 aaacaacgtg ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt
   108841 aagcaaaatc cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc
   108901 actttggatt acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa
   108961 aatcaatagt gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt
   109021 gtatcgacag gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag
   109081 ccttttgtct tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt
   109141 gctcgccgat acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct
   109201 ttttattcaa tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga
   109261 aaaattgaaa gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc
   109321 gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc
   109381 tggtgaaaac ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat
   109441 gctgagtaca atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc
   109501 aattttttct acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg
   109561 tgttttttgt atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac
   109621 tgattccatt atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata
   109681 aagcaatagt ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaacggca
   109741 atgaatgccg ctcttcttat ttcgattctt ttataattct gcttctgatt catctttggt
   109801 tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg attttttcct tcaacgcttc
   109861 gattgcctgt gcgccacgtg ccgcttgttc agcattgatg gattcaatga ccagcaccgc
   109921 tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca cgccagttga gacagcgaca
   109981 gatcgctcct tgttcatcat agtagatgat ttcttccgga agtgccggag catcggaatc
   110041 tgcacctaaa ggcaggaatg attcgccacc ttgggctttt cctaaatgca gatttcctgc
   110101 gatcttttcg atatcttctc cgccgcaagg tacgccaaaa gacaaggaaa tactattata
   110161 gatatctaca agcggattga ttgggaaaaa ttcccgttct tgatttgcac gtttcagtaa
   110221 agcttcaata gaagaacgtg cacctttttt agtcttgaat gtggtaaatg cctgacgcca
   110281 ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta aggtaatttc ttgcttcttt
   110341 tgctgcctca ttcaataatt gatgatacgt ttcttcactt tcttttgtta cgtggttatc
   110401 gataccattt acggataaga tttcgatggt tgcttcagga aatacttccc agaaattggt
   110461 atccactatt acttttttca tctgttcaca cctcttgatt agaataattg cttgatgaat
   110521 agatagtctg tttggggatc atctcccata taaaaaacat gttcgctgta tttgataaat
   110581 ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt cccaaacacc gagccaaatg
   110641 gctgtttttt gcttttcttt tgctttttca atagctgtat ctagtaaaaa tgtaccgata
   110701 ccttgatgtt taaatttttt tcgtacatag attcgctcga tttctaaaaa atgatcagcc
   110761 atttcctcag attgactcgt tcctgtattt agtttcaggt aacccgcagg ctcttgatcg
   110821 atactcacta ggaaaaaatc ggtttcttta tcttttagct cttctgtaag tttttctagt
   110881 gcataagctt gtgtcaagta atcagctaaa tgctccggtg aattatcttt tccaaatgta
   110941 tctgtaaatg tttcgatact aatttgttgt agtaagtcta catctgaagg cgtcattttt
   111001 cgaatttcgt atttattcac ggcttcttcc tttcaataga tccttttatt gccttttttg
   111061 acaaaattcc aatcttcgct aacattcttg catactttat ttaaaagata ttccaattgt
   111121 tttgcttctt cgtcagaaag accatttaat gcgactgcat tggagtagtt gttttctcga
   111181 atgatgaaag gatagatttc tgctcctttt ttagtcggat agagtttctt gattttttta
   111241 ttttctttat cttccaaacg ttcgatcatc ccattggatt ctaatttctt gattgctcgg
   111301 gcagtcgttg tacgatctac tttgatcatt tctgctagtt tttcttggat gattccagga
   111361 ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg ttaaatcgac ttctttgaat
   111421 tcgatattgc taatagaatc tagcgctcgg gcaatcattc cgatgtctcg aaggatttca
   111481 gccatttcat cccaccttta ttttgtttgt aagactagtt cacctctttt tgttgcaaat
   111541 gcaactattt tcaataaaaa aatcaagttc ttttttaata actagtaaag tatattcttt
   111601 tcttctttta cgaaaaagct caatcacaat agatctggtt tttgttttct aaaataagta
   111661 actgtttctt cgttgttcgc ttttctgata gctttggcaa ctcttctata ctaaaaaatc
   111721 ccatgttgga agtttcatta ttctcaataa acttggcctc ttcaccgtgg atcgcacaag
   111781 caaaaacaag tttataatac tgaaaaagct gtggaatatc tttttgttta ttcgtatcaa
   111841 aaactgctcg caactgagta gtttctactg ttaatcctgt ttcttcatag acttctttac
   111901 gcacattttc ttcaggagac aatcctattt cagcaaatcc accaggaagt gaccattctt
   111961 tcgtccgttt atcttcgatt agtaggacct tgccatcttt tttgatatag gcacgaacat
   112021 ctacttttgg tgttggatgt ccttctccaa tcgttgtgag cgcttttatt tcttcgaaag
   112081 tttcttttcc tatttcactg atcagttcca atgaaatcgt ccggatctcc tcaaaacgct
   112141 ctttatcaaa tacatctttt ccataatata gaccagcttg agaaagtgcg agtagtcttt
   112201 tgtattgagc caacttcttg ttcattctct tcccttcttt tttagcaaat gtatgactat
   112261 gtaatcaagt atagaagact ggtttaatta cggcaaataa atataatgaa atctgcaata
   112321 aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc gacttctctg gggctgtgaa
   112381 taaatggcgt tcaaaagctt gcatctgcgg taataagccc caccaaacga aaaatatgac
   112441 gagctattct tgtctggctg ttcttattaa ttgaggcagg acagcttttt cacaacctcc
   112501 ccactaaacg gtgttctcct accaactcct cggtcacaag tcacaatatt catcaaacat
   112561 gagaagctgt ttttttgaaa ttgctccttg tgactcggag ctgaacgaca ggctcacgac
   112621 ctctcggtaa acagcgttca aaagccaatt tttcgatatt aagccatttc tttgttactt
   112681 attgtcattg atttcttttt ctaatttatc caatagtgga gtgtaatcca cttgagtata
   112741 gtctgttaca tcaaattcat atagtgttcc taactggaat ttgtcatatt ttcggtcggc
   112801 gatgatcttt ttgaatgctt ctttcgtaat atgattggtc cctttcgttc gatcttccaa
   112861 tacgtcacca tagtgataat gatccatgat atagctcaaa tgacgatctg attcacgatc
   112921 acgtttgtac cgacgttgcc ataacacttc aaagtctgct gttaggcgaa tcgtgatcac
   112981 ctgatatccg tatctatctg ctaattcttg gagtcgtgct ttttgcttat cactaaaagg
   113041 atactcggac acaatcactt tctttcctgc ttccatatac aaatcaagtg ctttgtaata
   113101 gaaaggataa actttcgtcc tttcttgttc cgcccgttct tccaaattgt taaacccgat
   113161 ggattctgca tagtattcct tgatttcatc gagcgtgatg gcatacatat ctggaaatcg
   113221 ttcccgtagt ttattcataa gatatgtttt tcctgtacca ggactaccag ccagaagaat
   113281 gaaatacttt ttcatctcat ttatttccta agttaattgg actttccaag ttcataaaca
   113341 gtccaatctt atgcttgaca atttctttta ctgcttcatt tgctggaatc acattatggc
   113401 gaagagactt gttgatttct gtttcactaa aggtttcttt agctactttc gtccagttga
   113461 ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt attgatcaat tcaggataat
   113521 cttcttcttt cacacctgtt cctccatgga taactagggg gacatcaact actgcatcga
   113581 tttctttcag caagggaatg tttacttctg tttttgattt gaattgtcca tgattcgtac
   113641 cgatagcaat agctaatgca tccacgcctg tagcttctac aaatttcact gcgtcttccg
   113701 gtcttgtata taccttgtca ttttcagcga catggattcc ttcttctgta ccgccgattg
   113761 ttccaagttc tccttctacg gaaacaccgt gcgcatgggc atattctact actgcttttg
   113821 tcttcaggat attttctttg aaaggcagat gggacccatc atacatgaca gaagtatatc
   113881 cgctgtcgat tgcttctttg atatcatcaa agtctcgggc gtggtctaag tgcaagactg
   113941 catcaaccat ttcttcttct gcaatagctg tcattacatt gactaacaca tgcatgccga
   114001 tatatttagc ggtatcgaca cttgtttgaa taatgatcgg cgaacccatt tcttttgctg
   114061 cgcctagcat agcaggaagc atttctaggt tatgcgtatt aaaagctcca actgtaaaat
   114121 tcaactcttc tgctgttttt gttacttctt tcaatgttgt atacatcttt tattgccacc
   114181 ttatcttttt atttgtttcc tttgatcgtt acttgttttg caatttgtcg catctcatcc
   114241 attttgccca tgtagtaatc gatcccttga tcatcaccag cacgagcagc atctgcacgc
   114301 ttctggttat agaggctcat cagtttttta tacccatctc cgatcgtctg tttattttcc
   114361 aaactctttt ctaaatattg gatggcttga tcgttcacat acaattcaga aaatttaagt
   114421 ccaacttgtt cataaagttt cgctaattta tgcttatctt gggtagtgga aatctcttgt
   114481 ttcaattgtg taatctctga ttgaatcttt tcggtcgctt ctggcgatag ggctttttct
   114541 tcttttgcca gaactttttt cggttctttt tcttttttct taaaaaatcc aaacatcatt
   114601 ttccctactt tcaattaaaa tgctttcaaa actgggctta agatccaggc atataagagt
   114661 gctgcggcta ctgtatctac atcggttgct gtagcattgt tgaatcccat cgaattaaag
   114721 atcgttacta gcaaagcggg caataatgta ataaagaaac catgagcaat accgccaatg
   114781 atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc ctgctgtccc tccggcaaag
   114841 aaattggtta gcataccagg taaaatcatc gctaagccga acatgggcaa agtaaacatg
   114901 gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa tgacagcatt tgggctataa
   114961 ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag gaactaattt catagctatc
   115021 cctcgaaatg ccggcacgat ttcacctaac agtagacgta caccagctag caatacatac
   115081 acacccacta cgaattggat ggcttgtaaa aaggcaaaca tgatatagtt ttgatctcct
   115141 gataattcag aagcgaatgg ttcgcctgca aataaaacag tgatgatata tagcgggacc
   115201 atcacgacca tgacagacaa ataagtatct tgcagaaatt cgaaatgtgt cggcaacttg
   115261 atatcttcga tcgacttttt attttcgcct ttctcaccaa aaagcttagc aacaccggct
   115321 tcaaataagt acccgatcgt acaaaaatga cctaaagcaa tatcatttga tccagtgatt
   115381 ttacgaatga tcggttgcgc tactgccggc atagcaatgg cgaaacaagc tccgataaaa
   115441 ccaccgacca agatcaatac gatccctcgc agaccggcaa aatagccaaa gactgtcgtc
   115501 atcgttgcca tccataaaat tgcttgacca gttaaaaaaa tgtacttcca ttttgtgaaa
   115561 cgagccaaaa taatattgaa tacaaaaatg gctaagaacg tcagtgcaat atctctacct
   115621 aaacccaaat cattcattgc ttgaccattg atagattcaa tagaagggat gatcccttgc
   115681 atatgaaatc cttcagtaaa gattttcccg aaataagtta aacttccaac gatgatgctc
   115741 gaaccggcac tcaacacttg aaatcctagg agtgtcttta acgtgccaga gatgacttgt
   115801 cctgctgatt tcttctgcag tagtagaccc aacatagcaa tcaatgcaat ggtgatcgat
   115861 gcctgagtca aaatattttc gatgataaaa ttgataacgc ccatg
//
LOCUS       DAZKMK010000002       104220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000002 DAZKMK010000000
VERSION     DAZKMK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 104220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.002"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..313
                     /locus_tag="ACG8V1_000102"
     CDS             <1..313
                     /locus_tag="ACG8V1_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328235.1"
                     /translation="ILSDKINVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQ
                     VGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPL
                     K"
     gene            complement(394..963)
                     /gene="thiT"
                     /locus_tag="ACG8V1_000103"
     CDS             complement(394..963)
                     /gene="thiT"
                     /locus_tag="ACG8V1_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0328236.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1029..1131)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1137..2108)
                     /locus_tag="ACG8V1_000104"
     CDS             complement(1137..2108)
                     /locus_tag="ACG8V1_000104"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0328237.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2202..3575)
                     /gene="glmU"
                     /locus_tag="ACG8V1_000105"
     CDS             complement(2202..3575)
                     /gene="glmU"
                     /locus_tag="ACG8V1_000105"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0328238.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4078..4896)
                     /gene="purR"
                     /locus_tag="ACG8V1_000106"
     CDS             complement(4078..4896)
                     /gene="purR"
                     /locus_tag="ACG8V1_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0328239.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5063..5887)
                     /locus_tag="ACG8V1_000107"
     CDS             complement(5063..5887)
                     /locus_tag="ACG8V1_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0328240.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5860..6540)
                     /locus_tag="ACG8V1_000108"
     CDS             complement(5860..6540)
                     /locus_tag="ACG8V1_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328241.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6563..7501)
                     /locus_tag="ACG8V1_000109"
     CDS             complement(6563..7501)
                     /locus_tag="ACG8V1_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0328242.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7746..8597)
                     /gene="ispE"
                     /locus_tag="ACG8V1_000110"
     CDS             complement(7746..8597)
                     /gene="ispE"
                     /locus_tag="ACG8V1_000110"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0328243.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(8751..11324)
                     /gene="mprF"
                     /locus_tag="ACG8V1_000111"
     CDS             complement(8751..11324)
                     /gene="mprF"
                     /locus_tag="ACG8V1_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0328244.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11465..12271
                     /gene="proC"
                     /locus_tag="ACG8V1_000112"
     CDS             11465..12271
                     /gene="proC"
                     /locus_tag="ACG8V1_000112"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0328245.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12350..13906)
                     /gene="rny"
                     /locus_tag="ACG8V1_000113"
     CDS             complement(12350..13906)
                     /gene="rny"
                     /locus_tag="ACG8V1_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0328246.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14317..15366)
                     /gene="recA"
                     /locus_tag="ACG8V1_000114"
     CDS             complement(14317..15366)
                     /gene="recA"
                     /locus_tag="ACG8V1_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0328247.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15475..16716)
                     /locus_tag="ACG8V1_000115"
     CDS             complement(15475..16716)
                     /locus_tag="ACG8V1_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0328248.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(16956..17534)
                     /gene="pgsA"
                     /locus_tag="ACG8V1_000116"
     CDS             complement(16956..17534)
                     /gene="pgsA"
                     /locus_tag="ACG8V1_000116"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0328249.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(17743..18666)
                     /locus_tag="ACG8V1_000117"
     CDS             complement(17743..18666)
                     /locus_tag="ACG8V1_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0328250.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(19405..20691)
                     /locus_tag="ACG8V1_000118"
     CDS             complement(19405..20691)
                     /locus_tag="ACG8V1_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0328251.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(20684..21952)
                     /locus_tag="ACG8V1_000119"
     CDS             complement(20684..21952)
                     /locus_tag="ACG8V1_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0328252.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22204..22665
                     /gene="mscL"
                     /locus_tag="ACG8V1_000120"
     CDS             22204..22665
                     /gene="mscL"
                     /locus_tag="ACG8V1_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0328253.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(22724..23170)
                     /locus_tag="ACG8V1_000121"
     CDS             complement(22724..23170)
                     /locus_tag="ACG8V1_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0328254.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23263..23508)
                     /locus_tag="ACG8V1_000122"
     CDS             complement(23263..23508)
                     /locus_tag="ACG8V1_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0328255.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(23623..24201)
                     /locus_tag="ACG8V1_000123"
     CDS             complement(23623..24201)
                     /locus_tag="ACG8V1_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0328256.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24278..24808)
                     /locus_tag="ACG8V1_000124"
     CDS             complement(24278..24808)
                     /locus_tag="ACG8V1_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0328257.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(24812..25672)
                     /gene="rlmB"
                     /locus_tag="ACG8V1_000125"
     CDS             complement(24812..25672)
                     /gene="rlmB"
                     /locus_tag="ACG8V1_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0328258.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(25665..26060)
                     /locus_tag="ACG8V1_000126"
     CDS             complement(25665..26060)
                     /locus_tag="ACG8V1_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0328259.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(26057..27469)
                     /gene="cysS"
                     /locus_tag="ACG8V1_000127"
     CDS             complement(26057..27469)
                     /gene="cysS"
                     /locus_tag="ACG8V1_000127"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0328260.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(27466..28002)
                     /gene="epsC"
                     /locus_tag="ACG8V1_000128"
     CDS             complement(27466..28002)
                     /gene="epsC"
                     /locus_tag="ACG8V1_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0328261.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(28088..28327)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28324..29784)
                     /gene="gltX"
                     /locus_tag="ACG8V1_000129"
     CDS             complement(28324..29784)
                     /gene="gltX"
                     /locus_tag="ACG8V1_000129"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0328262.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(29954..31111)
                     /locus_tag="ACG8V1_000130"
     CDS             complement(29954..31111)
                     /locus_tag="ACG8V1_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0328263.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(31145..32515)
                     /gene="radA"
                     /locus_tag="ACG8V1_000131"
     CDS             complement(31145..32515)
                     /gene="radA"
                     /locus_tag="ACG8V1_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0328264.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(32578..33105)
                     /locus_tag="ACG8V1_000132"
     CDS             complement(32578..33105)
                     /locus_tag="ACG8V1_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0328265.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            33335..34141
                     /gene="proB"
                     /locus_tag="ACG8V1_000133"
     CDS             33335..34141
                     /gene="proB"
                     /locus_tag="ACG8V1_000133"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0328266.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            34138..35385
                     /locus_tag="ACG8V1_000134"
     CDS             34138..35385
                     /locus_tag="ACG8V1_000134"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0328267.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            35461..35805
                     /locus_tag="ACG8V1_000135"
     CDS             35461..35805
                     /locus_tag="ACG8V1_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328268.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(35922..36248)
                     /locus_tag="ACG8V1_000136"
     CDS             complement(35922..36248)
                     /locus_tag="ACG8V1_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328269.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(36863..38065)
                     /locus_tag="ACG8V1_000137"
     CDS             complement(36863..38065)
                     /locus_tag="ACG8V1_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0328270.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            38182..38697
                     /locus_tag="ACG8V1_000138"
     CDS             38182..38697
                     /locus_tag="ACG8V1_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0328271.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(38896..39342)
                     /locus_tag="ACG8V1_000139"
     CDS             complement(38896..39342)
                     /locus_tag="ACG8V1_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0328272.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(39389..40867)
                     /gene="msr(C)"
                     /locus_tag="ACG8V1_000140"
     CDS             complement(39389..40867)
                     /gene="msr(C)"
                     /locus_tag="ACG8V1_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0328273.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(41221..41850)
                     /locus_tag="ACG8V1_000141"
     CDS             complement(41221..41850)
                     /locus_tag="ACG8V1_000141"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0328274.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(41834..42298)
                     /locus_tag="ACG8V1_000142"
     CDS             complement(41834..42298)
                     /locus_tag="ACG8V1_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0328275.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(42785..44053)
                     /locus_tag="ACG8V1_000143"
     CDS             complement(42785..44053)
                     /locus_tag="ACG8V1_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0328276.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(44284..45021)
                     /locus_tag="ACG8V1_000144"
     CDS             complement(44284..45021)
                     /locus_tag="ACG8V1_000144"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0328277.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(45088..45657)
                     /locus_tag="ACG8V1_000145"
     CDS             complement(45088..45657)
                     /locus_tag="ACG8V1_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0328278.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            45785..46657
                     /locus_tag="ACG8V1_000146"
     CDS             45785..46657
                     /locus_tag="ACG8V1_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0328279.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            46999..47871
                     /locus_tag="ACG8V1_000147"
     CDS             46999..47871
                     /locus_tag="ACG8V1_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0328280.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            47868..49826
                     /gene="nagE"
                     /locus_tag="ACG8V1_000148"
     CDS             47868..49826
                     /gene="nagE"
                     /locus_tag="ACG8V1_000148"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0328281.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(50158..51009)
                     /locus_tag="ACG8V1_000149"
     CDS             complement(50158..51009)
                     /locus_tag="ACG8V1_000149"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0328282.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            51132..51599
                     /locus_tag="ACG8V1_000150"
     CDS             51132..51599
                     /locus_tag="ACG8V1_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0328283.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(51862..52344)
                     /locus_tag="ACG8V1_000151"
     CDS             complement(51862..52344)
                     /locus_tag="ACG8V1_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0328284.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(52444..53307)
                     /locus_tag="ACG8V1_000152"
     CDS             complement(52444..53307)
                     /locus_tag="ACG8V1_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0328285.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            53905..54780
                     /locus_tag="ACG8V1_000153"
     CDS             53905..54780
                     /locus_tag="ACG8V1_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0328286.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(54925..55077)
                     /locus_tag="ACG8V1_000154"
                     /pseudo
     CDS             complement(54925..55077)
                     /locus_tag="ACG8V1_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(55345..56676)
                     /locus_tag="ACG8V1_000155"
     CDS             complement(55345..56676)
                     /locus_tag="ACG8V1_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0328287.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(56825..57538)
                     /locus_tag="ACG8V1_000156"
     CDS             complement(56825..57538)
                     /locus_tag="ACG8V1_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0328288.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            57713..59137
                     /locus_tag="ACG8V1_000157"
     CDS             57713..59137
                     /locus_tag="ACG8V1_000157"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0328289.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            59436..60143
                     /locus_tag="ACG8V1_000158"
     CDS             59436..60143
                     /locus_tag="ACG8V1_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0328290.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            60130..60453
                     /locus_tag="ACG8V1_000159"
     CDS             60130..60453
                     /locus_tag="ACG8V1_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0328291.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(60542..61579)
                     /locus_tag="ACG8V1_000160"
     CDS             complement(60542..61579)
                     /locus_tag="ACG8V1_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0328292.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(61900..62883)
                     /locus_tag="ACG8V1_000161"
     CDS             complement(61900..62883)
                     /locus_tag="ACG8V1_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0328293.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(63298..64068)
                     /locus_tag="ACG8V1_000162"
     CDS             complement(63298..64068)
                     /locus_tag="ACG8V1_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328294.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(64061..64996)
                     /locus_tag="ACG8V1_000163"
     CDS             complement(64061..64996)
                     /locus_tag="ACG8V1_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328295.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65312..66961)
                     /locus_tag="ACG8V1_000164"
     CDS             complement(65312..66961)
                     /locus_tag="ACG8V1_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0328296.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(67078..69681)
                     /locus_tag="ACG8V1_000165"
     CDS             complement(67078..69681)
                     /locus_tag="ACG8V1_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0328297.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(69693..70742)
                     /locus_tag="ACG8V1_000166"
     CDS             complement(69693..70742)
                     /locus_tag="ACG8V1_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0328298.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(70768..71082)
                     /locus_tag="ACG8V1_000167"
     CDS             complement(70768..71082)
                     /locus_tag="ACG8V1_000167"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0328299.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(71098..71544)
                     /locus_tag="ACG8V1_000168"
     CDS             complement(71098..71544)
                     /locus_tag="ACG8V1_000168"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0328300.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(71545..73467)
                     /locus_tag="ACG8V1_000169"
     CDS             complement(71545..73467)
                     /locus_tag="ACG8V1_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0328301.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(73679..74575)
                     /locus_tag="ACG8V1_000170"
     CDS             complement(73679..74575)
                     /locus_tag="ACG8V1_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0328302.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(74568..75248)
                     /locus_tag="ACG8V1_000171"
     CDS             complement(74568..75248)
                     /locus_tag="ACG8V1_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0328303.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(75249..76031)
                     /locus_tag="ACG8V1_000172"
     CDS             complement(75249..76031)
                     /locus_tag="ACG8V1_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0328304.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(76024..76941)
                     /locus_tag="ACG8V1_000173"
     CDS             complement(76024..76941)
                     /locus_tag="ACG8V1_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0328305.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(77105..77359)
                     /locus_tag="ACG8V1_000174"
     CDS             complement(77105..77359)
                     /locus_tag="ACG8V1_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328306.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            78216..81443
                     /gene="fss3"
                     /locus_tag="ACG8V1_000175"
     CDS             78216..81443
                     /gene="fss3"
                     /locus_tag="ACG8V1_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0328307.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            81556..81870
                     /locus_tag="ACG8V1_000176"
     CDS             81556..81870
                     /locus_tag="ACG8V1_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0328308.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            81883..82257
                     /locus_tag="ACG8V1_000177"
     CDS             81883..82257
                     /locus_tag="ACG8V1_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0328309.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            82267..82602
                     /locus_tag="ACG8V1_000178"
     CDS             82267..82602
                     /locus_tag="ACG8V1_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0328310.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            82685..84025
                     /locus_tag="ACG8V1_000179"
     CDS             82685..84025
                     /locus_tag="ACG8V1_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0328311.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            84103..84777
                     /locus_tag="ACG8V1_000180"
     CDS             84103..84777
                     /locus_tag="ACG8V1_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0328312.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            84770..84934
                     /locus_tag="ACG8V1_000181"
     CDS             84770..84934
                     /locus_tag="ACG8V1_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328313.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            85010..86152
                     /gene="dcm"
                     /locus_tag="ACG8V1_000182"
                     /pseudo
     CDS             85010..86152
                     /gene="dcm"
                     /locus_tag="ACG8V1_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            86142..86432
                     /locus_tag="ACG8V1_000183"
     CDS             86142..86432
                     /locus_tag="ACG8V1_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328314.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            86691..87875
                     /gene="mobT"
                     /locus_tag="ACG8V1_000184"
     CDS             86691..87875
                     /gene="mobT"
                     /locus_tag="ACG8V1_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0328315.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            87872..88009
                     /locus_tag="ACG8V1_000185"
     CDS             87872..88009
                     /locus_tag="ACG8V1_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0328316.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            88755..90665
                     /gene="ltrA"
                     /locus_tag="ACG8V1_000186"
     CDS             88755..90665
                     /gene="ltrA"
                     /locus_tag="ACG8V1_000186"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0328317.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            90769..90993
                     /locus_tag="ACG8V1_000187"
     CDS             90769..90993
                     /locus_tag="ACG8V1_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328318.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            91006..91509
                     /locus_tag="ACG8V1_000188"
     CDS             91006..91509
                     /locus_tag="ACG8V1_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0328319.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            91569..91958
                     /locus_tag="ACG8V1_000189"
     CDS             91569..91958
                     /locus_tag="ACG8V1_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0328320.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            91945..94392
                     /locus_tag="ACG8V1_000190"
     CDS             91945..94392
                     /locus_tag="ACG8V1_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0328321.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            94397..96520
                     /locus_tag="ACG8V1_000191"
     CDS             94397..96520
                     /locus_tag="ACG8V1_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0328322.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            96517..97521
                     /locus_tag="ACG8V1_000192"
     CDS             96517..97521
                     /locus_tag="ACG8V1_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0328323.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            97540..98451
                     /locus_tag="ACG8V1_000193"
     CDS             97540..98451
                     /locus_tag="ACG8V1_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0328324.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            98638..98894
                     /locus_tag="ACG8V1_000194"
                     /pseudo
     CDS             98638..98894
                     /locus_tag="ACG8V1_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(99111..99326)
                     /locus_tag="ACG8V1_000195"
     CDS             complement(99111..99326)
                     /locus_tag="ACG8V1_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328325.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(99575..99793)
                     /locus_tag="ACG8V1_000196"
                     /pseudo
     CDS             complement(99575..99793)
                     /locus_tag="ACG8V1_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(100088..101527)
                     /locus_tag="ACG8V1_000197"
     CDS             complement(100088..101527)
                     /locus_tag="ACG8V1_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0328326.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            complement(102115..102747)
                     /locus_tag="ACG8V1_000198"
     CDS             complement(102115..102747)
                     /locus_tag="ACG8V1_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0328327.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            complement(103096..>104220)
                     /locus_tag="ACG8V1_000199"
     CDS             complement(103096..>104220)
                     /locus_tag="ACG8V1_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0328328.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT        31313 a        21008 c        19036 g        32863 t
ORIGIN      
        1 gatcttgtca gataagatca atgtaaaaaa atcttttttc aaaaaagaaa cacctgttga
       61 aaaaatcagt gtggaattat tgaaagaaat gcgtaatttg atgcaggaag ggatcatttt
      121 ttcttttgat caagttggtt tgatcgatgg taaatatcgt atcccaatct atgtgattga
      181 agattatatt tggaaattaa aaggcacccg gtatcgagaa gaggaaatca tcgctacttt
      241 aatctactct gtcaccgatc gacactggca attggtagaa gatgccgatc tttcttatct
      301 gccattgaag tagatctatt tcttatcaaa aaataaaaag gaaacaagaa actaggacat
      361 gattctgtct caagtttctt gcttcttttt tatttacagc tcctgtcgtt tatctggcac
      421 aaaaatcgaa ggagtcgttt gttggatccc taataaaacg atgatcgtta caatagctgt
      481 actcaacccg ctggcaccat tcattacgaa agaaaagagc caaggattca tcccccatag
      541 tgcatacgct ccccagaaaa ccactcctgc aataaaatgc caaaaatatc tggaaagtgc
      601 gccaatcaat gcaccaaaca cgatgctttt tctaatttgt ttcgtattat cgtctacata
      661 gcctttttgg acatttgaag ccatgagccc tgctgcgcct gcaaaagtat aggcaatcgg
      721 gtactcaatc aacacttgaa cgacacttaa ataataaact tgtcccaacg gaaaatgtag
      781 gatgccccaa atgaaacctg ccagcatccc tgcctttgtc cctcttctta acgcaaaaag
      841 agtcaatgga atctgcccta atgaaataga aaaactgctg ccgatctgga tcggaatcaa
      901 cgacaacacc atcgctaacg ctgcaacaat cgttccttct acccaaactc tattttttcc
      961 cataaaaaaa cttcctcctt tgaataaaaa agtcacaaag gaggaagcag ctttcgaaaa
     1021 tagtcaaaca gctgtttatt cacaattcct acgctcgcat aaccgataca ggtacgaagg
     1081 gtttagaaat agattcaatc tcagcccaat tggactcccc tttgtgagtt tatttattat
     1141 ttattttttg tttcaaacaa tggactaact ggtttgtttt catggatacg tttgattgca
     1201 tctcccatta gtccgccaac acttacttca tcgattttgt cgattttacg atcttctgga
     1261 agatagatcg agtctgtaac aactagacgc tcgatagctg aatcttctat tctttgtaaa
     1321 gcagggccgg ataatactgg atgtgtacat gaagcataca cactagttgc accagcttct
     1381 ttcaaagcat tggcagccaa tgtgatcgta cctgctgtat cgatcatatc atcgatcagt
     1441 acacaagttt tgcctttgac ttgtccaatg atattcatta cttctgcaac atttgctttt
     1501 ggacggcgtt tgtctatgat cgcaatcgga gatttcaaaa attccgctaa tttacgtgcc
     1561 cgtgtgacac caccatggtc tggtgaaaca acgacaacat catcgccttt gattcctttt
     1621 tctaagaaat aattggcaat aagtggcgca cccatcaaat gatcgactgg aatatcaaag
     1681 aaaccttgga tttggactgc atgaaggtcc aaagtcagca ttcttgttgc accagctttt
     1741 tgaatcatgt ttgctactaa tttcgctgtg attggttccc gtgcacgtgc tttacggtct
     1801 tgacgcgcat agccataata aggcatgact acgttgattg tttttgcact tgctcgtttc
     1861 agtgcgtcaa tcatgatcag caactccatc aagttatcat tgactgggct gctcgttgat
     1921 tgaataacat agacatgcga tccacgaata ctttcttcaa tgtttacttg aatttctcca
     1981 tcactaaatt gagtaacaga tgattttcct aactcgacgc cgactgctgc tgcaattttc
     2041 tctgctaatg ggcgattgga atttaacgca aaaattttta atcttgggtc aaagtaatgt
     2101 tttgacatgg ggacctccac tttctttttc acaactctag aattctacct aataaatagt
     2161 agtcatattt tttaaataaa tcaagtgatt ttgcaaaagt cttagtttag ataagggagt
     2221 ttttttgcat atccttcttt attgacttgt cttgctctcg caattgccag cgcatattct
     2281 ggaatattgt ctgtaattgt tgacccggca gcaatcgaac tgtttttagc tacctcgaca
     2341 ggtccgatga tattcgtgct agaaccaata aagctgtggt cgccgacagt cgtatgatgt
     2401 ttgttctttc catcatagtt gacgaagaca acaccacatc cgacattgat ttcttctccc
     2461 agcgtagcat caccaacata agtcaagtga ccgactttag ttcctttgcc gatttgagca
     2521 tttttcactt ccacaaagtt tccgatatgg acgttttcgc caatttcagc ttttggtcgt
     2581 aaatgtgcat aaggtcccac atctgcatgt ttcttgacat ggctgctttc aatgactgag
     2641 ttttcaatga cgacgtgatc ctcaatccta ctgtctacga tttttgaatg tgacccgatg
     2701 acacaatcag aaccaatgac tgtatttcct tggatttgta cacctgcttc gatcaatgtg
     2761 tctggaccga tttctacgcc tgcatcgatg tatgtcgttg ctgaatcgac aaagcttacg
     2821 ccattgacca tatgcatctg gttaattcgc tttctcatga tctcatttgc tttagctaac
     2881 gcgatccgat cattgactcc cattgattct tcaaaatcat ccgtttgata agcagcaact
     2941 gtatggcctt cttcttttaa gatttcgatg atatctgtta gatagtattc accttgagca
     3001 ttgtttgttc caactttgct taacgcatcg aacaaagctt cgttgtcaaa acagtaagtt
     3061 cctgtattga tttcttgtac aagagcttct tctggtgttg catctttttg ttccacgatt
     3121 ttttctacga tcccaatatg gtcacggatg attcgtccat atcctgttgg gttttctgct
     3181 tgtgccgtca agatcgttgc acttgcattt tttccttggt gatattcaaa aagattgttc
     3241 agcgtttcag tagtcaataa tggtgtatcg ccactgataa ctaacgttgt tccttttttt
     3301 ccttccaaga aagaagcagc ttgcaaaaca gcatgtcctg ttcccaattg ttctgcttgt
     3361 aacgcatatt tactgcgttc tcctagttgt gctttaactt gttctgctcc gtgtccaacg
     3421 atcgtaatca cttcatctgg tttcgtttca ctgacccgat taatgatatg ttctaccata
     3481 ggtttaccag aaacgggatg taaaacttta tataattttg acttcatgcg ggtacctttc
     3541 cctgccgcca aaataattgc ataacgtgcg tccaactgcg tcactcctaa atatagtttg
     3601 ctgatttcag tttatcccag tatacccatt ttttcaacaa aacaagtgat tttcttttat
     3661 cccagcgttt tctggataaa aaaatagata gaccataacg ggcctatcta tttggtaacc
     3721 ggtgctctcc tgccaactcc tcggtcacaa gtcacactac tcataaaaca tgaaaagctg
     3781 ttttttgaaa tcgtttcttg tgacttagag ttaaacggta ggcttacaac ctcttcatta
     3841 aacgtttttc aatcagctga ccttcggtat tagctcaata aacggcaaaa tatgaggagc
     3901 aatttttccg tttattgagc taatactcag gacaggacgc tgatttcaca acctctagat
     3961 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgaaaa aatgtggaaa
     4021 gccattttca caaaattttt cttactactc gaaactaagc ggctttttca caacctctta
     4081 ttctgaaaaa taatttcctg gcacaaccgt aatatttttc gtttgagtat ctacatcttt
     4141 gacataaagc agtgacgtat aatcatcgat ggcgcgatga ccgttgaatt ttgattcggc
     4201 aaatacagtg atgccaacaa gttctgattc gaattcttca atcaagctct tcattccgtt
     4261 gactgttccg ccgcctttca tgaaatcatc cactacaagg acacgtgaac cacgtttcaa
     4321 acttctctta gacagttcca tcttttcaat tctttcagaa gatccagaca catagttgac
     4381 acttactgtt gatccttctg ttatttttga gtcccggcgt acaataacga atggcgcatt
     4441 cagataatag gacacagctt gggcaatagg tacgcctttt gtggctacag tcattacagc
     4501 atcgattttt tcaccaaggt atttagaagc aatgattctt ccaacttgac gcagcagttc
     4561 aggttctcct aacaaatcag agagataaac atatcctcct ggaagcagac ggtcttgttc
     4621 tgatagacgt tctgctagag attcaatata ttttttcgcc tcttcatatg aaatttcagg
     4681 gatgaacaat acaccacctg cagcacccgg gattgtttcc aaggttccat accctcgttc
     4741 tttaaatgtt ttcttgatga ttcccaaatc ttcactgata gaagatttag cagattcata
     4801 acgttcggca aagcttgtta aggaaatcaa ttcatgcggg tgattcaata aatactgtgt
     4861 catgtcaact agacgttcac tgcgacgtac tttcacctat tccaactcct tttaagaaat
     4921 tttatcgttt tacattatat aagtttttct tcaaaaattc gagcatttta tgaaaaaata
     4981 agaaaatagt tcggtattta ttaaaataaa aagaagccta taaaaaattt ttcgttttta
     5041 taaggcttct tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt
     5101 tgatgatatt tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag
     5161 gtgtatctag ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa
     5221 cactgatagc aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa
     5281 gaaccatgat tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca
     5341 caccagtaat cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg
     5401 cggtatcttc atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac
     5461 cgatggcaac gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg
     5521 aaccaaacag ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata
     5581 ctaacgctag cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc
     5641 ggtagatcgt tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg
     5701 taagtgtagg attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag
     5761 aaagcgtatc agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg
     5821 cgattccggc aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca
     5881 acgccattga gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat
     5941 atcttcatga tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg
     6001 gagaagacga taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag
     6061 aacaaataag tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc
     6121 gccagacaat tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc
     6181 acgctgtaca tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata
     6241 tcttcctgaa cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac
     6301 ttgttgagga atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt
     6361 tatcattcct tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc
     6421 tgctccattt tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc
     6481 ttctagtaca ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac
     6541 cttacaacct ccattctaaa acttaattga tactttcttt taaagctttc agattttttt
     6601 ccatgacaga aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag
     6661 gattcaacac ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag
     6721 aggcattttc ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct
     6781 cagctaagcg acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt
     6841 ttaagtcgta ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt
     6901 ttgttgcatc ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat
     6961 aagcatcggc attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt
     7021 cagcaatcgt tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt
     7081 attcatgtga atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt
     7141 ctaaacggtc gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat
     7201 cctttaacga atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat
     7261 cggaaatttc tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag
     7321 ggatcaaaag tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg
     7381 gataaaaagt cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc
     7441 cacatgctgc aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca
     7501 tttatatgct cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg
     7561 tatcaaaata aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata
     7621 ttcttgatta aatcgtaaaa tggatgagtt gctgctcata tctagccatt tcactacaat
     7681 aaaaaactga agaataccat tttaggattc ctcagttttt catcacctat tttacttctt
     7741 atattttatt gcagggttcg tactaaataa acttcctcac aaaaaccttt taacccatta
     7801 tatactctct gcgctcttga ataatgttga cacaaggcaa aaacggttgg tccgcttcca
     7861 ctcatcaatg cagcatctgc tccgtacttg atcatacgct ctttgatttg atggatgact
     7921 ggatgccgct ttcccgttat atcttctaaa ctgtttccca tttttttgat catctgctga
     7981 taatcttgct ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc
     8041 gtatctactt ctctaaacac tgtacgtgta gagacactca ttcttggttt gaccaacaca
     8101 acccagcact gtggcataga aggtaatact tggatttctt cccctttgcc accgacaaat
     8161 gccgtacttc cgtagacaca gtaaggaaca tctgtaccga cttccaaacc gatttctgcc
     8221 agttcttcta acgaacagcc cagattccat aattggttga tccctcttag agcagctgca
     8281 cagtctgtac ttcctccgcc taatccagct gctactggaa tattcttctt aacaaagatc
     8341 ttgacaccgg ttttgatatt gtatttattt tttagcaata aagcagcttg ataaacattg
     8401 ttccgttcat ctaccggtaa aaatgcttta tttgtttcta caatgatttt gtcttctgtt
     8461 atttcttcaa aaaatagatg atctgccaag tctacgctcg acatcaccat ttccaactca
     8521 tgatacccat cttgccgtct atataataca tccagtccta aatttatctt ggctggcgct
     8581 ttttcaatca attccatcct ctcacccatt cgaaaagtct aagtcgcaag tattctatca
     8641 tagggaaaaa taaaagtata gcttatgtct gcctagtggt tgattttgtc atcgtttcaa
     8701 cagctttcac agaattttct cttcataatc caaacaagtt ctagtgattc ttataattct
     8761 gtgagcactt cttctgccaa actcggaaca tttccttttt cttttcctat cagactgagt
     8821 agctgcatgg tagcaaatcc gaaattactt cctttcctat aagcaaaata acgtccttcc
     8881 caatgatcga catattttaa tttcttttca taagcagact ggaaggaata gaaaaaatta
     8941 ccataattat atacgatatg catgactttt tcttgaaaga aagaaaaatc tgtttctcca
     9001 acattagcta atggagctaa gcctagattg gcttccgagt agcccatttt ctcgtataca
     9061 ataaacagat tggctaacag atagtcagct aattcctcag attctttagg taagacccgc
     9121 aacaaatcat atcccgcctg cttcttcgtc atcggctgag cggtgatgaa gccaacgact
     9181 ttttgatctt tacggacaat accgacaccg ctagataata catatgattt atcaaatcgt
     9241 cccacagaga agtatttttc tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca
     9301 gcttgatgta attcttccgg cagttgatcg taccaagtaa aatggtatcc ttctttcatc
     9361 aaacgttgat attcaagttg ctgactcagc cgagacaagt cttctgttgc tagatcaaca
     9421 atccctgctt cacctacttt cgtgaagtga aaacccaatt catgaagcat cactacgtag
     9481 ctttctgata tcttataaaa cgcaagctga tagcctaaac aatctgcttg tttcataaac
     9541 tgattcgttg ctgcccgcat cattttagga ttgccaactg gatctcctaa aacaaagcat
     9601 ttgtttcctt tgatctgata accaaaaacg acttgatctt ggtcgtcttc ttggtaataa
     9661 aaataactgt aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc
     9721 aataaacggt caaaacggtc tttttcgtac tcactgccaa gcttcgtagt atcttcagct
     9781 aaatattgat aaagcgccac taaagctagc aatgagatgc ccaaaccaat aagccctgag
     9841 aaccacacat catctgaagg aaacaaaata aaatgatttc ctgttattcg accgactaaa
     9901 aagcgtccgc tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc gaacaagcta
     9961 gcatcaaaga ttatagctcc ccaagaataa acgaattttg ttcgataaaa ttcttttctg
    10021 gataaccaca cagctaacag aatcaagaca tataccaata tcaactgcca agataacgta
    10081 cgtgtgattg tatttaaaat accaaaagat agtaaagcaa tcgtaggcag aaatgccttt
    10141 ttgactttta ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac caatagattc
    10201 actgtctgat ctaaaaaatt gaatgagaac ggcaataata cttgaaatag ccgactgatt
    10261 gcagacagat tagtgattgt tgatagaagt accatcatga tccccgcaaa atagacagca
    10321 cttacgagca cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat
    10381 cgattgattt tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga
    10441 acataataaa acaaccgata atataatagc caaactactg ctacatcttg ccgaacagac
    10501 aattgactca ttcctaagat catcaaaacg tcaaaagtcc ccattccacc tgggaccatc
    10561 gtcagcattc ctatcaatgt agcaatcatg aacataggat agatcgaaag catcgaaata
    10621 tctattctca tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt ccattgtcca
    10681 aaggatgcca gtagcagacc aagcaagcct tttggataaa actcgaagaa caatgtcttt
    10741 ctctttagat aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag
    10801 atccaatagc ttcgaaaaat actttctgac tgaataaaaa agacatcaat aaatgtaaga
    10861 aacgaccata cagacaaacc agtcagcata aatagtgcga cttttgatac agtggctaaa
    10921 accatttttc tatttgtctg ctttccataa aaactagccc gcagacttgc tcctaccaca
    10981 ccaccaaaac cggcgagatt gttgatcgtg tttgttaccc acgcagcaag aagaaactct
    11041 tttctttcca tttttggttt tccttgtttt tctaagactc tgatcgtcac ccaatcatac
    11101 aacaacatcg gagtgacacc cagcagtcca gccagaatca tgagcaggat cgtcatgcgg
    11161 ctttgttgtc ccatcctttg ccaaatatct tgccaactca tgccattagc aatatttgcg
    11221 acttgatttg ccacaaagac taacacggag ccgaaaaaaa tcagttttaa aatcagacta
    11281 tgttccttta tccattgcag catcaagtat cgtttatttt tcacattttc ccattcctaa
    11341 cttgtatact tttgtgtcat accccaatgt tatcttattt attttaattt ttccacttta
    11401 gacctgttct ttgcttgcga tgatagaaaa agaaagatat cctaatgcta aaggagagga
    11461 taacatgaaa gtaggattta tcggcacagg gaatatggca caagctatta ttgatggttt
    11521 attaaaaaaa ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa
    11581 tgcacaaaaa tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc
    11641 atcacaatca gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa aagtattgga
    11701 agaactaaga gaatatagcc agaaagtctt attcatttct attgcttcag gaaaatcatt
    11761 aatagattta gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa
    11821 tgtcaatgtc agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga
    11881 ggaacagctt cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct atcctttagc
    11941 agaatcagat ttcagtacat tcattggcct tgctggcagc tcacccgcat tcgtttacat
    12001 gctgatcgat gcgatgggac ggactggtgt actgaatggt ttgtccaaac agacagccac
    12061 tgagattgct gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa
    12121 tccatgggaa cttgtagacc aagtttcttc tcctggcggt acaactgttg caggtgtagt
    12181 tgccttagaa gaagcaggat tcatcccagc aatcatcaaa ggaatcaata cgacgatcga
    12241 gaaagatctt gagatgttga ataaaaaata atgatagcaa tgttttttcg tgccttacaa
    12301 aaaaagaaga gctgtggctt ttgccgcggc tcttcttttg tattttttat tatttagcat
    12361 aatcagttgc acgagtttcc cgaataacga ttactttgat gtgtccagga taatccagtt
    12421 cttcttcgat tcgtttacga atatcacgta ctaaacgaac agcgtctaaa tccgagattt
    12481 cttctggctt gaccattacc cgaacttcgc gtccagcttg aactgcgtaa cttgattcta
    12541 caccggcgaa gctgttcgaa atattttcca gacttcttaa acgacggatg tagttttcta
    12601 atgattcact tcgcgcacca ggtcttgctg ctgagagcgc atctgctgca gctacaagga
    12661 cagagataac agaagtagct tccacatctc catgatgaga agcgatcgcg ttgattacta
    12721 ctgggttttc tttgtacttg gcagctaatt ccgcaccgat ctcaacgtga gatccttcaa
    12781 tctcatggtc aagtgctttc ccaatatcat ggagcaaccc ggcacgtttt gctaattgga
    12841 tatcttcgcc aagctctgct gctaagatac cagctaattt tgcaacctcg accgaatgct
    12901 tcaagacgtt ttgtccataa ctagttctga atcgcagacg tccgatgatc ttgattagat
    12961 ctggatgaag tgtatgagca ccgacttcaa acgctgcctc ttccccgtat tcgcgaatac
    13021 gttcatccat ttctttacga gatttctcga ccatttcttc gatacgcgct ggatggattc
    13081 gtccatcttg tatcagtttt tccaaggtca tccgtgcgat ttctcttcgg attggatcga
    13141 atccggaaag aacaactgct tccggtgtat catcgatgat taaatcgata ccagtcagcg
    13201 tctcaagcgt tcgaatatta cgaccttctc gtccaatgat acgacctttc atttcatcat
    13261 tcggaagggt aacgactgaa acagtcgttt ctgaaacttg atctgctgcg cagcgttgaa
    13321 tcgctaatga taataagttt ttcgctttgc gatctgcttc ttctttggca cgctgttcag
    13381 attcttttac cattagagtt aactcatgat tcagctcttc ttcagtcgat ttcatgatga
    13441 catctcgtgc gtcctctttt gataaaccag caattttttc tagttctttt tgttgatctt
    13501 caatcatttt ttcaacgtct ttttctcgct cttcaattaa ttgctgtttt gaactaagtg
    13561 cttcttcttt actttcaagt gagttctcgc gtttttctaa cgagtcatct ttacgattca
    13621 gcgtttgttc gcgttgaatc aaacgattct cttgagattt taattctaac ttactttctc
    13681 tcagctcact ttcaacttca gaacggtatt tctgattttc ttccttagct tctaacaatg
    13741 cttctttttt tagagtttct gcttcttttt ttgcactatc gatgattcca gcagctgatg
    13801 attgtgcacc agctatttct ttctcgtgac gtgactttgc gaccataaac cctaaaccaa
    13861 gaccgacaat taaaccgatg atagcgagga gaatgttgaa taccataaaa tccacctcca
    13921 tactatcttt ttcattttta tcatctatac agcttctatg ataaattatt ctaaactcgt
    13981 caatatgcat cttcgcatat atgtgtacaa aaaacatgca caactttatt ctaatgtttg
    14041 taccccgtag tgtcaacttt aaataaacaa agaaacggct ttctacttat tcattaaacg
    14101 atggttttta aaataaatga cactagtttc atataggtaa acctttaaat tgagaaactt
    14161 ttctattaaa ttattctttg tagatgaaca gatattttta ttttatgtac ccactcgtat
    14221 aattaaataa ctcacgattt ctttattcaa taaaaaaacg gcaggtcaag gagagtacct
    14281 actcttcaga ccagccgttt ttgtttatca taatttttat tcttctacta aaggcaattc
    14341 ttcttgtcct ttttcatctt ctgggacagc gacttcttca ccaataccat aagctgctct
    14401 aactagagca gaaacttctg ccatcatttc aggatgatta gccatataga ttttagcgtt
    14461 ttcccgtcct tgaccaatcc gatcttcttt ataagaatac caagcaccgc ttttatcaac
    14521 aatgtctttt tctactgcca tgtctagaag ttctccttct tgtgagatcc ctaaaccata
    14581 catgacatct acttcagcga ttttgaaagg aggtgctact ttgtttttta cgactttgat
    14641 ctttgtacgg ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac gacgtacttc
    14701 caaacggatc gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt ctggattcgc
    14761 aaacatcacg cctacttttt cacggatttg gttgatgaaa atagcgattg tttttgtttt
    14821 gttgatcgaa ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac
    14881 atgtgagtca cccatttcgc cgtcgatttc tgctcgtgga actaaagcag caactgaatc
    14941 gactactacg atatctacag ccccacttga tactaaagca tcagcgatct ctagaccttg
    15001 ttctcctgtg tcaggctgtg aaagaagtag ttcatcgata ttcacaccta atttttgtgc
    15061 atattgcgga tctaacgcat gctcagcatc aatgaaagcg gccgttccgc catttttttg
    15121 tacttctgca atagcgtgta gtgcaactgt tgttttacct gaactttcag gtccatatac
    15181 ttcgatgata cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta acgcaagaga
    15241 gccacttgga attgtagaga tttgttggtc gattttttcg cccaatttca taatagagcc
    15301 tttaccatag ctcttttcaa tttttttcag tgcagcatct aaggctgctt tacgatcatc
    15361 tgccaatacg ataatcctcc ttatatttac aaatcagagc aacatttcat tatccttatc
    15421 atatcttttt tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca tttatcattt
    15481 tcttaaaagc tgttttctca aaagatcaaa tccttgcata accgcattat atctgatagc
    15541 agcacgatct ctggcaaagt gatatttttc cgaaaccacg ccgtctttag atgaaatagc
    15601 gatccatact gttcctgcag attgaccttc taacgtgtcc ggtccagcca ctccggtgaa
    15661 agctacactg taatctgttc ttgctatctc acgtgcttga atggccatct gctccacaca
    15721 ttctttgctg actgttccat attctgctaa tagctccgga tcaatctcca gaaaaccagc
    15781 ttttgtttgg agcgagtagg taacgaatcc gcctggaaat acttcagata caccagcaat
    15841 gtcgcctaat gcggcttgga aggcgccggc tgtcaaactt tccgctgctg ttacagtctg
    15901 cttgttttct ttcagtagtt ccactacgac ttttgctagc gaattatcat cgccgtatcc
    15961 ataaaaatat tcccctactc gttcttggat tttttcttct aatgctaaaa gagcttgatt
    16021 ccctgcctga acatcatttg ttttcactgt cagtcgtaat gtcacttcat ttggtttggc
    16081 atatggtgcg atcgttggat tgatctgtgt ctcaatcaga tcttttagct cagtgaccag
    16141 tcgcgattca ccaatcccat aaaagcgcaa aactttagaa attagttttt cttcagaagg
    16201 aaaacgttcc tctagtaaag gacgtacttg ttccacaaac attggtttca actcattagg
    16261 cggaccggga agaaggatat acgcatgtgt atctgtttga taaaaagtcc ctaaagcaag
    16321 ccctgtacga tttggtagag gaaccccgcc ttcgataatc tgggactgtt gcagattgtt
    16381 tttggtcatt tttcgatggg ttgtttcaaa atatgccaat aattttttgt atccttccgt
    16441 attttgtatg agagacttac cgaggtgagc cgcagttgct tcttttgtca ggtcatcttc
    16501 tgtcggtcct aatccgccac ataagagtat gagttcactt cttgtttcag ctagagcaat
    16561 caactcttct aaacgctgct gattatctcc tacaactgtt tgataataaa cttcgatacc
    16621 tagatcagct agctgttctg acaagaaagt ggcattcgta ttcacgactt gtcctagtaa
    16681 aagttctgtt ccgacagcta ttatttctgc tttcattact tcccgctccc ttatatagta
    16741 tacgctgtat ttttatattt ttttatttaa aaagagggca aggagctgtg acaaaaatct
    16801 tgtcacagct ccttgttcca aataaacagt gttctcctgc caaaacctcg gtcataagtc
    16861 acaatataca taaaacatga aaagctgttt tttgaaatta tcccttgtga ctcagagtta
    16921 gacggcaggc tcacaacctc tccactaaac gatacttaca ttgatccttt gaacacaccg
    16981 ctgtttttga tgaagtaatc cacacctgaa tagatagtaa agatcagaca agcataaagc
    17041 atgatttgat ctactggaat accgatcagg ctgaatggga tattattgat aaacagcaag
    17101 ataatagcaa ccatttgtgt agccgtcttg acttttcccg gccaagctgc agccatgact
    17161 tctccatgtt cgacaagtag taaacgcaag cctgtgacgg ctaattcacg acagacaatg
    17221 atcgctacta cccaaccagg ggcctttcct tggtcaacca acatgataaa agcagtcata
    17281 actagcattt tatctgccaa cgggtccgca aacttgccaa aattagtgac aaggcctcgc
    17341 gcacgggcaa tttttccatc taaccagtct gtgatactag ctaaagcaaa aataactgca
    17401 cctactagct gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc ccaatctaaa
    17461 ggtaaaccta caaccaaaat gaaaatcggg atcatacaaa ttctaagtac agttaattta
    17521 tttggtaaat tcacactatc gactcctttc ttccctattc tacgtgacta tcagcgaagt
    17581 ttccaccgat attatttact tttccaaatg aatttattca aaggagtatt tttattgaaa
    17641 ttgatctttt tgaattaacg aagaaggacc gcgacacaga tttgtcacga tccttctttt
    17701 ttatacctac aagtaaacag atgcgtaaac taatttatac gatcattctg cgtattgaag
    17761 agtaaggttc acattttttg gactctgatt gttttcccct ggattaactg gtacggattc
    17821 tccattcgct ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg catttgttgc
    17881 tgtttccggt aacacggtac tctgtgtttc atttgctgcc agtgtccctt gataaacata
    17941 ggcattgtct actagcacac ctaaccatac acgattgaca gcagtaaatt caagggttac
    18001 aggcttggta gcgtctgtga tatttactgc gatggcactg ccagaatctt ctcctgtagc
    18061 tattgccatt ttcttttctt cacttgattt tgttgttgat tctactgtag aagactcttt
    18121 ggtctgcgtg ctttgctctg aggaagagga ctcctcgact gatccttcta ctttaagcga
    18181 agaagctgtg tctccgatta ttggatcagc ttgacgatct tgccaagtca tatagcccac
    18241 gacaacgaca atcgccaaag caaccaatcc taataaaatg acaggaagac ttgtccaaaa
    18301 gcgacttgga tgcgattctt cctgatgcat tgcttttcta gaagtactta ctgtttcagg
    18361 ctgcggacgt tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa catcgactaa
    18421 atggtctcca tcttctccta cagcttcagc gtattgccga ataaatgccc gcacgtaaaa
    18481 tttaccaggc aattggtcaa aatcatcatt gtcgattgct tccaaataac gtttttgaat
    18541 ttttgtaatc tgctgtaatt catctaaaga tattttttta ttcaggcgtg cttgccgtaa
    18601 tttttctccg atggtttcaa aagccactcg ttcatcttct ccagtctgtt tgattctccg
    18661 aagcatctgc tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa agtcatgtca
    18721 cagctccttt tttccgaata aacattgttc tcctgccaac tcctcggtca caagtcacaa
    18781 tatccaataa atataagaag ctgttttttg aacttgttcc ttgtgactcg gagttaaacg
    18841 gcaggctcac gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg tattagctca
    18901 aaaaccggca aaatataagg agatattttt ccgtatgttt ctctaatact caggtcaaaa
    18961 cgctgatttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    19021 tccaaccaag ttaaaaacat taagaagctg tttttacttg gctgttctta ttacttgatg
    19081 cagggcagct ttttcacaac ctcttggtac acggtgttcc agaagtagct tcttcggcaa
    19141 taagccgaaa tatcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    19201 aagctgacca cttctgtcac aacctctttg attacttcgt ttgattttat tttaatataa
    19261 gttgattaaa agagagcatg atttgtttta ttttctcact aatttttcca acacaactaa
    19321 gaataccata aaagctgatt ttggaggagg ttcctactac aagtctaaaa cgaaatttaa
    19381 gacttttcct cataaaatac tggtttattt tggtctcatg taaaactcgc tgaaggcctc
    19441 ttcacttata aatgcttccc cagctgccag tacgtctttc atttggatcg aatcgatgat
    19501 ttcaggaaga tcaaataaag ttcgatcgcc aaataaactt tgcgtaaatt gattggcaat
    19561 atattcgatc gaatttaatg attggaaata ttgtccaagc attttcttct tcagcaaatc
    19621 gagatttgtt tctgacactt ccggatcatc agcgaatcca agaatgattt ctttgacttt
    19681 ttctgctgct ttctctggtt cgtccgtatc tccgctgaag tccgcaaagt gaaattcacg
    19741 atccaagctg aattcaaaac caaagctgtc atcgataatt ccttgattgt acatcgctag
    19801 atagtttcga gaagtattgc ctaaaatcat ttggaacaac agattgattg ctgttttata
    19861 gcgtaacagt tctgttcctt cttgcggcaa tgtgtctaat cctttaatac caagtacaaa
    19921 tttatctcgt gtaatggctg cttccaatgc actttgtttg atgatttctt ttgtgttttc
    19981 agggaaataa cgtacaattt cttgctttgg agggaaattt tttgcttctt ggttttcccg
    20041 aataagtttc atcaatttct caggctccat cttaccaact acaaataaaa ccatattgct
    20101 tggctgataa aacgtccgat agcacgtata caagtcttga gctgtgattt tgtcgatact
    20161 ttcaaccgtg cccgcaatat cgatatgcaa tgggtgtgta ggatacaagt tatttagtat
    20221 gccaaaaaac atccgccagt tagggtcgtc ttcatacatt tggatttcct gaccgatgat
    20281 tcctttttcc ttgtttacag tttcttctgt gaagtaaggt gcttgtacaa aatcgatcag
    20341 agtcgtcaga ttcttttcta cctgatccgt cgtagaaaac agatagctgg ttttcgtaaa
    20401 acttgtgaaa gcattggcgg aagctccttg tttcccaaat aattgaaaga catcaccatc
    20461 ttctttttca aacaacttat gttctaaaaa atgggcaatt ccatctggaa ctttcacctt
    20521 ttctttttca ccatagggaa taaattcgtt atcgatcgaa ccataattcg tactgaacaa
    20581 accatatgtc ttgtgatagt catttttagg caacaaatac acggtcaaac cgtttggcag
    20641 tacttcatga taaagtgttt cattgatttg ctcatattct gttttattca tttgcatttc
    20701 ctcctgctaa aaagaagatt gcctgcaatt cgatttgctc agctacttgt tgtatctctt
    20761 tgatcgttac gctctccata cgtttcaacc attcttcatc agataatttt gtttccggca
    20821 gccagctgtc caaaaaagaa gcttcgattg ccgcttgcgg agagtctaga gaaagcagat
    20881 attggtttct cagcattgct tttgtctgag acagttccaa ctctgttatt tcaccatttc
    20941 gcaaactttc taattgctcg tgaatcagat gaagtacttg atttcggttc ttttcatcga
    21001 taccggtttg gacactcatg aatcctcgga acgtatccac gctactagag gcataataag
    21061 ctagactttc tttctctctg acattcatga aaagtttaga gtgaggaaaa ccgccaaata
    21121 aaccgttaaa gaccattagc gcaaaacgtt ctggttcatc gtaatacaca tttgtctgat
    21181 aagctaggtt cagctttgct tgaacgattg gctcacgtac ttgtcgttcc ttcacgatat
    21241 tcacttgagg ttgggtataa aacagatctg gataaactgt ttcccgcgac tcaaatggta
    21301 attgcccaac tgcttcagct gctttgtccg gatcaacatc gccgacaaca aaaatgtcga
    21361 tttgatcatt tgccatcatt tcttgatagt aggacgctag cgaactagca gtgatcttgt
    21421 ccaaatcttc gactacgccc aaccctggga tcttttgttc cgggctgttt tcaaaataaa
    21481 gttcttgcaa ggcataggaa gcaaaagttt gtttatcttc cttcaaactt tccaaatata
    21541 gacgcagatt gttcttttcc aagtcaaacg tcaactgatc aaactgatga tttttaatat
    21601 ttggataaaa taaaacctct tttaaaaaat ctaccgcttg agaaaaaagg tttggatcat
    21661 tgacatattc accattcaca aaactgatcc ctgcgtttag ccaatgtaaa tttccttttt
    21721 ttctaactga taatccaaag cttgcgccat aaagctctgc aagttgcgca cttaactttg
    21781 tctgatccga ataattcaaa ctatttgttt ccaacatgct gctgagaagt gaacgtttcg
    21841 tgatgacttc ttgttgcaga cgcgcagtaa aacggataaa aatccggatc gttttgtatt
    21901 gttttgttgg taagacatga agattcacgc cttctgccaa ttgatacacc atatttatat
    21961 gctccttttc acagttgatt gcccgcttac tcttttaatg ggtcaacctg ccttttttac
    22021 ttattcctct gcttgaaatt tataagttca ggcaattcgc cgttttttct aaatgatgtt
    22081 acactttata gtatagcata gatgtttttt atcaaaagaa acagacggct aaaagcgtat
    22141 attgtttttt tattttttat ttttcgatat actaaagaca aataaaagaa gggagggtca
    22201 ttcatgatca aagaattcaa agagttcatc atgcgtggag atgtcttgga tcttgctgtc
    22261 ggcgttgtta ttggtagtgc gtttacaagc atcgtaaacc aagtggtgca aggcttgatc
    22321 acaccactaa taggttggtt tgtcgcaatg atcacaggta cttctgattt agaaggagca
    22381 ctttcaattc ttgactggtc gccgacaaaa ggcgtcacat ttgcgtttgg taacgttatt
    22441 agtgctatca ttacatttct gatcactggt tttgtcttat tcttagttgt caaagcggca
    22501 aaccgtgcga agaatttacg ctccaaagaa gagcaagtgg aagtggaaga aactcctact
    22561 gcagaagatt atcttagtga catacgcgat cttcttgcca aacaagtggc agaagacaat
    22621 acacatccag tagagcaaac aaatctttcc gatacttcta aataataaag aatctaaaaa
    22681 aaagaaagcc gattttcagt gtcggctttc tttttttaga ttcttaactt gctacttgtt
    22741 cctttttcat gtcgatatgt ggaatatcat cttccaaata cacatctgag attgccttga
    22801 atccaaacga ctcataaaaa gcttgtaggt attcttgtgc gccgatcacg attggttttt
    22861 ctggaaaatt tttctggatc atgttaatcg tttcttgaac aatctttctt cctaaaccat
    22921 ctttacggtt gtcctgatga acaaggacac gtccaaaatg gatactctcc gcttcttcat
    22981 atactcgcgt gtaggcaagg atcttcccat cagtatcttg aaaaaacgta tgccaagctt
    23041 ctccatcaat gtcatcaact tcttgataag gacactgttg ttccacaaca aaaactgcga
    23101 ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa ttcttcaaat ttctttacct
    23161 gatattccac taagcaaacg ccccctgctt ttttattctt agcataccga ttcgcacaca
    23221 aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct ttttaaactg cttcagttaa
    23281 aaattcgatc tcaactgtcc gagtaagtac atctgaatag ctgtaagata ctcgttcaaa
    23341 agaattttct tcaggatcca aatcaacaac gaatactgaa ggataggttt ctgttaaaac
    23401 gccgcggcgt tctgtttgac gctttcttcc tgtctgagca accaacatga ttgggctgcc
    23461 gatacgacct tctaaatctt tcttgattga agctaaagtt gttggcatac agttcacctc
    23521 tactatcaac tataccacat atttttaaaa ttttcaagtt taccataaaa aaaaaagaaa
    23581 tgcaataaaa ataaataaga agctcattaa gatttttcta aattattcct cattataaaa
    23641 tttcaagtgt ttttttattt tgagttttac tctcccgtaa gcgctcatta cttgcttgtg
    23701 atccattgct aaacattcag ccatgtctac aaaatcaacg ccttgcatat aataatgaaa
    23761 aattttagct tctatttttg aaaactggat ttcgcaattt tcgattgctt cttttaataa
    23821 aagatgctga attgctaaag gttcttgcaa tgtgatctct gctaaaaagt ctggtccttc
    23881 tgtctctatt ttttggtcga ttgagacttc atctttttga tcacgacgct tcagcgcgtt
    23941 ctgcttcctc aacagagaat agatttgatt gcttataacg cttttataat aacttccaaa
    24001 gctaacacct ttgctttttt cgtaagttct caatgcctga tataaagcga tccttgcttc
    24061 ttgttcccaa tcctcagaaa cataatttct taaataatag gtgtttttca aacgtagtaa
    24121 aagtggaaaa tatttttgat acagggcagc aaatgcttct tcgtcaccat tacgtgcagc
    24181 gcgcaataac tcctcagaca taaaataatt cctccataaa ttcgtttatg gaggaatctt
    24241 atcatcaatg attcatcgaa ctgattagaa cgatatgtta tttatttttt ctcattaaat
    24301 cgtccaattt ttgggacaat ttttttaact gctcttcatt ccatggagaa ttacgtcgga
    24361 aatcaataaa atgcatatct ttattttctt gggctattgt tttttccgtt ttttttatcg
    24421 tcttatataa ttcataagca gaagttcgta atgctccttg ggaaaagatc atccactgtt
    24481 ctgctaaatc gcttgtcgcc actgtcactt gtgtcaaacg gtcattcagc tgaccagcga
    24541 ttctctcgat ataactgtca gccgtttcgt cttctttcgt aaaaacaaca tccaactggt
    24601 atttcttata attttgttgg atgcccggaa ctagttgggc atcgaagaca acgatgactt
    24661 ccattccttc atatttcgca taatttgata aacgatgtaa aagagcttct cttgcatccg
    24721 ccattttgtc ctgcttttta agctggacaa gttccggcca cgaaccaatc atgttgtagc
    24781 cgtctacaat caacagctgt tttttcatgg tctactcccc ctgacgttgg gaaaaagcct
    24841 gatacataag caaaccagca gcaacactgg catttaagct ctggacgtgc ccggtcatcg
    24901 ggatcgtcag caattcatcc atttcttttt tcaatcctgg actcattcct ttgccttcat
    24961 taccaatgac taaagcaatt gatcctttgg cattccattt ttggaagttt gtgccggtca
    25021 tatctgtgcc aaatacccaa aatcctgttt ctttgagctg ttgcacagct tgtaccaaat
    25081 tcgtcacgcg tgcaacaggg acatgttcga ctgccccagt ggaagtcttt gttacgatag
    25141 gtgtgattcc aactgctcgg tgttttggta tgatcacacc atcgacgcca cttgcgtctg
    25201 ctgtccgtaa aatcgaccca aaattgtgtg gatcttcgat gctatccaaa atcaaaaaga
    25261 aggggtcttc ttttttctct tttgttcgtt caatcagttg gtcaagggat aaatattcat
    25321 aaggtgtaat cgcaagaacg actccttgat gcacgccgtg atcgctcaaa gtttctaatt
    25381 tttgtttagg aacccatttg actggcacag cttgttcacg tgcgatttct ttcagttctt
    25441 ctactttttc tcctcgtgaa tcttcttgaa gaaagagttt attccctcgc ccttgttgta
    25501 gcgcttctac tgcggcatgg tgcccaaaga cgaaattatc ttctaatgct tcttcatttt
    25561 gatcagaaac tgactgattt cgtccagttt gctttttatt tgaatatcta ttttgttttt
    25621 tgtcttcaaa tccacgtttc gattgatgtt tgtttgttct ttctttaccc atttttttca
    25681 ccaactttct gaatacacca gcggatcaat tcttccatcc gctcggtttg ttttgtcaaa
    25741 tgcagatagc ccatgagcgc ttcgaaccct gttgcgaccc gatacgtcgt aacatcagca
    25801 ttttttgcgc tcgtatggct tttagcattt cttcctctgc gataataaag ttcttcttct
    25861 tctgtcaaaa gatgttcttc cagcatttgc tgaatcaaaa aggcttgtgc attcgcagaa
    25921 acataattcg ttgccgcacg atgcagctta ttgggttttg tttctccttg atcaatcagg
    25981 tggtcacgaa catagatttc gtaaatcgca tctccaacat aagccaaggc taaaccgttc
    26041 aattgtgtgt aatctctcat gcttctcttc tccatcgggt tccttgcggt gtatcttcta
    26101 ataagatccc tttttctttc aacaaatcac gaatttcgtc gcttcggaca aaatcacgat
    26161 tgcttcttgc ttgatttcgt tcttcgatca attgttcaat ctcttcatct aataactctt
    26221 ctgctgtaaa taaaataccg aagatcgaaa gccattgcga gaataaagct tctccttgtg
    26281 ccaagacagc ggcagaaact ttcggctggt ctagataagt gttcagccac ttagcaagct
    26341 catagaccac tgtgatccca tttgctgcat taaaatcatc gtccatttct tcgatgaatc
    26401 gctgttctaa attttctaac aaagcgattt tatccgcatc atctgccaaa ttctctgagg
    26461 cagtttcttg gcggaatttc aaattgtcaa acgtattctt cagtcgttgt aaattggtgg
    26521 ctgcttcttt catcgtcgtt tcactgtagc ggatcggacg acggtattgt gtggtcgcca
    26581 tgaagaaacg caagacttgt ggatcaacgt ttttgatcat ttcatgtaca gtgatgaaat
    26641 tccctagaga tttactcatt ttttcatcgt cttcgccaat cgttacatag ccattgtgca
    26701 tccagtaatt agcgaatttt ttccctgttt tcgcttcact ttgtgcgatt tcattttcat
    26761 gatgaggaaa ttctaaatct tgcccgccac catggatatc gatcgtttct cctaaatgtt
    26821 ttgttgccat aacggaacat tcaatatgcc aaccgggacg tcctttgccc catggtgaat
    26881 cccaagaaat ctcgccaggt tttgcttgtt tccataaagc gaaatctaat gggtcttctt
    26941 tttgctgctg ttcttcccct gtacgctgac ttgctccgac ttccagttca tcgatggatt
    27001 ggtcacttaa tttcccataa ttttcaaatt tacgggtacg gtaatacaca tcacctgaag
    27061 attcataagc atagcctttt tcaaccaaca cttgaataaa tgctaaaata tctggcatat
    27121 gatccatgac tcttggatgc agtgtagcag gttcgacatt caacgctttc gtatcttctt
    27181 caaatgctcg gatgaaacga tcagcaactt ctggtgctgt aatacctaat tcgtttgctg
    27241 tacggatgat tttatcgtct acatccgtga aattcgatac gaaatcgact ttatagcctc
    27301 gatattcaaa atagcgtcga attgtatcga aagcaatggt gctacgagca tttccgatat
    27361 ggatataatt atacacagtc ggtccgcaga catacatacc gacatgtccc ttttgtatag
    27421 ggatgaattc ttcttttttt ctggtcaatg tattgtagat ttttatcatt ctgatctcct
    27481 cactttttct cctttgattc gaacgacttt tgctggtact cctaccgctg tagcgtcgtc
    27541 tggaacatct ttcaacacaa cggcacctgc accgattttg gcgttttttc cgatcgttac
    27601 cggtcctaat atctgcgcat tcgcagagag catggctcct tgtttaacag ttgggtgtct
    27661 tttacccgtt tcttttcctg tgccgcctaa tgttacgcca tggaataaaa tcacatcgtc
    27721 ctcgatttct gcggtttcgc caatgacgat tcccattcca tggtcgatga acacgccagt
    27781 tccaatcgtt gctcctggat ggatctcaac accagttagg aaacgccaga attgagcatt
    27841 gatcttcgcc aataaaaaga gccggtgctg atataaaaaa tgagagaaac gatgccaaaa
    27901 caaggcatga aaaccagggt atgtcaaaac gatctcagct gtcgaacgtg cagccggatc
    27961 gtttctcttt gctgcatcaa tcgctcgggt aaaccaactc atgactttcc actcctttcg
    28021 taagaatttt atgtaggaaa tcatcttctt ataaagaaaa gaagcgcctt ttaaataagt
    28081 ttccttatca aaagacgctt tccgcgtggt tccacttttc ttcataatca atgattatct
    28141 ctgacagcta acgcctgtta tacgttcttg gctactttgt ttcctataaa acagtttttc
    28201 ctgttttctg tgtttcgcca agaccactcc cagaggcatt tcggcaacca ttcattgata
    28261 ccttgcacca cccggtatct ctcttgtaat gatcagctgc ttactttttt ctgttcatcg
    28321 tgtttagttc agtacttgat ttaaatgtgc taatgatttt tcacgaccaa gcaattcgat
    28381 cgtttcaggc aattctggac catgcatttg tcctgataca gctacacgga ttggcatgaa
    28441 aagatttttt ccttttacgc cagtttcttt ttgaacagct ttgatggcag ctttgacagt
    28501 tggagcatct actacttcca tttgttctag ctgtgctttg aatgcgtgga gaacagtagg
    28561 aactgtttca ccagcaagta cttcttttgc tgcttcattc aatacaggat gttcattgaa
    28621 aaatagcgta gacagatcaa cgatttctgc agcatagctc atttgaggtt gatacaggct
    28681 aacgatcttc ttcacccatt cgattgtctt ttcatctggg ttttctgcta cacgtccatc
    28741 agcgattaga taaggcagac acatgtcagt aaatgcatct agatccattg ctttgatgta
    28801 atggttgttt acccattgca gttttttcgc atcgaaagct gcgggtgatt tacttaggcg
    28861 ttttggatcg aacattttga tcaattcttg gtgttcgaag atttcgtctt cgcccacagg
    28921 tgaccagccc aataatgcaa taaagttgaa cattgcatct ggtagatagc ctaattcgcg
    28981 atattgttcg ataaattgca agattgattc gtcacgtttg cttagttttt tacctgtttc
    29041 actgttgatg atcaatgtca tatgtccgaa ggttggtggt gtccagccaa atgcttcata
    29101 gatcatcagt tgttttggtg tattggcaat atggtcatct ccacgtaaaa cgtgagtgat
    29161 tttcatcaaa tgatcatcga ccgcaacagc aaaattataa gttggcatac catcgcgttt
    29221 ttggatgaca aagtcgccac cgatattatc ggattcgaac gaaatagcgc ctttcaccat
    29281 atctgtaaat gcgtattctg tattgcgagg cacacggaaa cgtacaacag attctagacc
    29341 ttgtgcttct ttttctgctt gttcttcagg tgtcaagttc gcacatgttc ctgcgtaatg
    29401 aggcatttct cctcttgcgc gttgtgcttc ccgttctgct tctaattctt cttctgtgca
    29461 atagcatttg tatgctcgat tgctagcaag cagctgatca atcagcggtt gatagatttc
    29521 tttacgttca gattgacggt aagggccgta ttctccagga ttttcaggag attcatccca
    29581 attcattccc aaccaagcaa ggttttccaa ctggcttttt tcgccatctt cgatattacg
    29641 tttttggtca gtatcctcaa tacgaataat aaagtctcca tcattgtggc gggcaaacaa
    29701 ataattgaat aatgcagttc ttgcatttcc aatatgcagg tgcccagttg gacttggtgc
    29761 atagcgtacg cgtactttcg tcattttctt cctcttcttt caaactagac tttgactaac
    29821 agcaaaaatt ccgtcagtta cggcttctca aatatcattt atttaaagcc actcagcaca
    29881 aattttacct tttctaaggt aaatagtaaa gccaactggt tgaatttcct taccagttgg
    29941 ctgaatttct tttttatttt gattgtttag tctttttctc tgtctctttt ttattgtcga
    30001 ttcctcgtcc tgcatgtgca ggtttagcaa agatcatgcg tccagcagct gtttgaaggg
    30061 cacttgttac aacgacttcg atacgttcat tcatgtaatg ctgtccatct tctacgacaa
    30121 ccatcgtgcc gtcatcaagg taggctactc cttgttggcg ttctgtcccg gcttttacta
    30181 ctagaacatt catcgtttca ccagggatga cgactggttt tacggcattt gccaacgcat
    30241 tgatattaag aacaggtaca ttttggaatt cagagacttt attcaagtta tagtcattgg
    30301 ttacaatgac accatctaaa agcttagcca atttgatcaa tttgctgtct acttcactga
    30361 tatcttcaaa gtctccatcg tacatttcga cagatatacc ctcttctttt tgcaaggcat
    30421 ttaggatatc taatccgcga cgaccacgca cacgtttcaa actatcccct gagtccgcga
    30481 tgtattgtag ttcgtaaaga acaaaattag ggatcatcaa ggttccttct aagaatccag
    30541 tctttgtaat atcataaatc cgaccgtcaa tgatcacgct tgtatccaag attttatatt
    30601 tatggaaatt ttctcctgat tttcgttcta acagttgact ctcaacttga ctagctgttt
    30661 cttgctcgat ttttttgctt cgtgaaccaa aaactttctt ccattcttcg attcgtgtcg
    30721 ttcccattct caatcccaga tagcctaaga tgatcatcag taaaatcggc aatacactat
    30781 tgacgaatgg aatttccata ttgtaaagag gggtcgagat cacaacacct aaagtcaatc
    30841 cgatgatcgc accgatcgca ccgaagagaa gataagtcaa gctgacctca cttagttttt
    30901 gttctacttg tttgataata gctgagatat gtttggccaa cagcaaagat aataaaaaga
    30961 aaataagtgc gcctaacagg ctgttggtaa atgtattatt cagccatccg tttgactgtt
    31021 gaccgattgc ctgccaagct gagggtaaaa atgaaatacc caagcttgcg ccgacaatga
    31081 tcatcagcaa agtaatgaca cgtttttgca tcgtttttcc tccgtttcga caatcattta
    31141 ttagttagcg gaacacacgc ttcaacgttt cagccaaagt tgctactcca actatctcga
    31201 ttccttttgg cgcttcccag ttacctaagt tgtttttagg taggtatact tttgtgaagc
    31261 cgagtttttg gacttctttg actcgttgtt caatatgact gacacgtctg atttctccag
    31321 tcagtccaat ttcaccgatg aaacattccg ttggctgtgt tcctttttct ttatagcttg
    31381 aagcaatact gacagcgata gctaaatcaa tcgctggttc atttaatttt accccacctg
    31441 cagcttttaa gtaagcatct tgattctgaa gaagcaaccc agctcttttt tccagcactg
    31501 ccatgatcag agatacccga ttgaaatcaa gacctgttgt tgttcgtttg gcatttccga
    31561 acatagtagg tgtgactaat gcttggatct ctaccaaaat tggacgtgtc ccttccattg
    31621 ccacaacgat agcagagcca gttgcaccgt ctaggcgttc ttctaagaaa atctgtgaag
    31681 gattagcgac ttcttccaat ccatgctcat gcatttcaaa aatcccaatc tcattcgttg
    31741 agccaaatcg gttttttact gctcgcaaaa tccgaaaact atgatgttgt tcaccttcaa
    31801 aatataacac cgtatccacc atatgttcta acattcttgg gccggcgatc gagccttctt
    31861 tggtaacatg accgacgata aaaatggcta tcccatttgt tttagcggtt ttgagtagtt
    31921 ctgcagtggt ttctcttact tggctgacac ttcctgccac acttgtgatg tctggctgcg
    31981 tcatcgtttg gatggaatca atgataacat aatcagggga gatatgttcg atggcacgac
    32041 tgatttcgtt catatctgtt tcagcgtata gataaaattc tgtgttgata cttccgaggc
    32101 gttccgcgcg taattttatc tgctctgcgc tttcttcgcc ggatacatat aagacttttc
    32161 cgcctatggc tgctaattgc tgagaaactt gtaacagcaa agtggatttc ccgatacctg
    32221 gatcgccacc aatcaagacc atagaccctg gcaccacacc tccgcctaac acacggttca
    32281 gttcttctaa ttttgttttg atccgaggtt cttttttagg gacgacgtct gcgatttttg
    32341 ttggttttgc tttttctcct gtcaaacttg tccgagttct gcggtccgtt gtatcttgtt
    32401 cgatttcttc gaccatcgta ttccattttc cgcaatttgg gcagcgtccc aagaacttag
    32461 gcgacacata cccgcaattt tggcaaacaa attgtacttt tgattttttt gccattgtac
    32521 cgcctccttt tatcctaatc atgagacatt aaatcaagct taagtatctt taacgtttta
    32581 tttactggaa gaaccaaatc cgccttgacg ttgagcagtc gtttgatctt gatctgcttt
    32641 taaaaacggc aaaaagatcc cttggccgat acgctcgcct tttttgatca ctacatcctt
    32701 gaaaccaaag ttggtgaatt ggaacatgat atggccttca tttgcctcat tattgtaata
    32761 atcactgtca atcacaccta ctccgtttgt caacactaaa aaccgcttca acggattgct
    32821 tgaacggtta gcaagctgca aaaattcatc ttctcccatg taagctttga ttccagtagg
    32881 cacgagggtt ggtttcaatt gtttttcatt ttctgataca tcttttttca atccatctgc
    32941 gactgtttcc aacccctttt tccagcctgc tttccagata cttggaatca cgacctcttc
    33001 tgctgcttca aaatcatagc cagcagcatg atgtgtagcg cgctgcggta gattgatatt
    33061 ttgatcttta tatttagaaa taacttcaaa tcctcttttt ctcaccggtt ttcctcccta
    33121 aataaactac tcctattcta acatatccga ggctgtaaaa gactttcttt acaaattctt
    33181 caagaaattc tgctatctta aagtaaacca ttaattcgtt ctttttccaa acgactgcaa
    33241 gttgtacaaa aaaactattt tttacatgac tttactcgtc aaaacaagta gaatagaacg
    33301 aaattaatga gtggatattt gattgaggtg tcacatgcgt aaagaaatta aaaatgcaaa
    33361 acgaattatt gtcaaagtag gaacaagtac attgatttat cccaatggaa atatcaatct
    33421 tgcggcaatc gatcagttag cttttgtcct ttctgatctt agaaatcggg gaaaggaagt
    33481 gatcttagtc tcatcaggag ctgtaggcgt tggtatgcat aaattacatt tgtcagaacg
    33541 tccgactttg attcctgaac agcaagctgc ggcggctgtt ggtcaagccg aactgatgaa
    33601 tatctataat caaagattca gtgtatataa tcaacagacg gcacagatcc tcgtgacaag
    33661 agacgtggta gattatcctg aaagtcgtaa aaatgtgagg aatacattgg aacagctttt
    33721 gattatggac acgatcccta tcgtgaacga aaacgatact gtagcagtgg atgaattaga
    33781 tcatctgaca aaatttggcg ataatgatca gctgtcagcc atcatggcaa aacttgtcgc
    33841 ggcagacctt ttgatcgtcc tttctgatat cgacgggttt tattcagata atccgactac
    33901 aaatcccgag gctcagatgt atcgctatat cgataaaatc gatgaaaaga ttctggcaca
    33961 agcaggtggt gctggtagca catttggaac aggcggtatg cagagtaaat taaaagccgc
    34021 ttctaggata tttgaagcaa acagcgcaat gattttagca aatggaaaaa atccggcaat
    34081 tatttttgat attcttgcag gaaaagaagt aggtacttta tttaaggagg acttgaaatg
    34141 actgacttga tgcaattagg acaacaggca aaagatagcg cgcaacaact agcacttatg
    34201 cctacaaacc gcaaaaatga gctacttgtt caaatggcac aaaccatcaa aaagaatcaa
    34261 caagcgatca ttgaagcaaa tcataaagac ttagaaaaag cagtcgaaaa caatatcagt
    34321 gaaacgatgc aagaccgctt gcagctgaca cctgagcgaa tcaatgccat ggctacagag
    34381 atcgaaaaaa ttgcttcgtt agacgatcct attggaaaag tagatgagat gtggacgaac
    34441 accgacggat tgagaatcgg caaaaagcgc gtgccactag gcgtgatcgg catcatttac
    34501 gaatctcgtc caaatgtcac gacagatgca gctagtctag cattcaaatc agggaatgcc
    34561 gttatccttc gcggtggcaa agaagcattc ttctcgaatc aattattagt ccaattattg
    34621 cagcaagtac tggtcagtgc tggcgaatct ccttatgcca tccagtttgt cgatgatact
    34681 tctcatgaaa ctgcaggaca gttgatgcga ttgacagaat acttagatgt gctgattcct
    34741 cgaggcggag caaaattaat ccaacgagtc aaagaacagg caacggtacc tatcattgaa
    34801 acaggtacag gaaataatca cgtatatatc gataaagaag cacagttatc tatggcagtg
    34861 aacatcatcg tcaatgcaaa agcttcccgt ccatctgtct gtaacgcggc agaaacattg
    34921 ctgatccatt ctgagatcgc accttttttc ttgccagcta ttgaaaaaga actggtagaa
    34981 cacggggtct ctttacgtgc ggatgctcgt gcgttagaat atctagagac ggctgcttta
    35041 gcagaagaca cagattggga tactgaatat ctagattata tccttgctgt caaagtggtc
    35101 gattctttgg atgaagcaat tgcacatatc aatcggcata atactaaaca ttcagaaacg
    35161 atcgtgacag atagttatgc ggctagtcaa cgtttcttaa atgaagtgga cgcggcggct
    35221 gtctatgtca atgcctccac tcgttttaca gacggatcgg tatttggttt tggtgcagag
    35281 attggtatct ctactcaaaa gttgcatgcc cgtggtccaa tggggttaaa tgaattaact
    35341 tccacaaaat atatcattta tggcgatgga caaatccgcg aataatgttt cgtgtgaaac
    35401 attattcccg ccttttgcct tttttctgta aaggttctat ccaactagaa aggtgtcaat
    35461 atgtttgaaa ttttaatgat tttttttatt taccttattt ctctgaatat cgctgctttt
    35521 ctgggagtcg gtattttatc ccttttcttt caattcaaaa aacgaagcat agggagtcaa
    35581 cgggaaaaat ggtcgcagta ttttgacaaa atcgggcaaa aagggctggt tacacgcttg
    35641 cacattagtt acatggttgc attatgctta ttagcgacaa tcaattacta ttcttttttc
    35701 gatcattcaa tcgcttatac gattacgcta ctgattgctg ggatttttca cttatcttat
    35761 aaatatcagc tgaataaaaa ccacttgaat cggactttcc gctaaaaaat atccgtacga
    35821 taagtagagt gccttgaaga tcggcaaaaa actgccggac tttttagagg ctgtgacaaa
    35881 gattttgtca cagcctcttt ttccatataa acggcgttcg cctaccagct gcctggtaaa
    35941 tctgtttcct cttcgttaag aagcgaaaat ccgtcaaatg tttcgccatc ataataacga
    36001 agcagctcat ccatggacaa gttcatcgtt ttttcttttt cttcttccgc tgattcagcc
    36061 agtagctgtt cccgatacac ctgatacata ccagctattt tttgctgcat cgcttttcta
    36121 gtttcacctt ccgcattgat tattggcaat tctttgatat agccacgata aatcaaccga
    36181 tcatctgaaa attcaatggg taattcaacg agtgtcaacc cgacttcctg aaaatattct
    36241 tccagcatct attatccccc cattcttttt cttaaatgaa ttttagctta cctactgaaa
    36301 attttccagt ttcattattt tgagaaaaaa agggaatgtg acaaaaatcg tgtcacatcc
    36361 cctttttccg aataaatggt gttcttctgc caactcctca atcataagtc acaatattca
    36421 acaaacatga gaaactgttt tttgaaattg ttccttgtga ttcagagtta aacgacagtc
    36481 tcacaacctc ttggtaaacg gcgttcaatc agctgaccct cggtattagc tcaaaaaacg
    36541 gtaaaatatg agaagctatt tttccgtttt gtttctctaa tacttaggat aaaacgctga
    36601 ttttacaacc tctgttaaac ggtgttcaat cagctgtcct tcggtattag ctcaaaaaac
    36661 ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggac aaaacgctga
    36721 ttttacaacc tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    36781 aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tatgacttga tgcaggacag
    36841 ctttttcaca acctctgatt tcttattctt cttcatttgt agcaaaataa ttacggtgat
    36901 aaatgataca acatacactg ataagaacaa tggcacctgc aaaataaaac gcatagcttt
    36961 caccaatatg ttcaccaatc acacctgcaa taaatggtgc taaagctcca ccgaaaaagc
    37021 ggacaaaact atagctggac gaagcaacgt ttcgttgcag acctggtatt tccatagcaa
    37081 tcgtcgtcaa cagtgtattg ataattcctt ggaaaaatcc tgctaacaca atgctgatcg
    37141 gtacaaggac tggcatagaa gttccagctc ctaataacag caaacaaata atgaataaga
    37201 caaatgctgc taaaattgtc cgataagtcg taaacttccg ctcaagagcc ggtgcaacga
    37261 aaatcgaagc aatagccaga agcacacccc agccaaaaaa tacaaaaccg acttctaatt
    37321 cagaatagcc aatcaataaa aatggcgcat acgcaagtaa agtaaagaat ccgaaattat
    37381 ataacaatgc gattagtcca actgaacgaa gtttatgatt gcttaacgct ttgacacctt
    37441 cccaaaggcc tacttttctc tttggtttct cagtttcagg taaaagtacc gctagggcta
    37501 taaaaccgca aaacatcaaa aatgctaccc caaaaaatgg ataacgccat gaaccactgc
    37561 ccaagacccc accgactaga gggccaacag ccattccgca gccaagtgct gcttcataca
    37621 tcatgattgc tttttctgtg ttaccaacta aaatactgac gatcgttgct aaagcagtag
    37681 agacaaataa agcattcccg acaccccagc cggcacgtaa cccaatcagc attccgatat
    37741 tcccagataa tccgccaaga ccagcgaata caacgatgat aaacaatcct gtcatcaatg
    37801 tttttttcaa accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga
    37861 ctaacatgta actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag
    37921 caatcgtttt taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag
    37981 ctgctacaac tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat
    38041 ctatttgatt ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt
    38101 ttacatgtca atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat
    38161 aagacgagag gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta
    38221 tccaaaccac gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat
    38281 cacagtcaga tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg
    38341 gacaaagacg agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt
    38401 gtagaggaat ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca
    38461 aaaatctatg ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga
    38521 gaacaacagc tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta
    38581 gacaagaaac aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag
    38641 caggaactcc aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg
    38701 ataaacagaa cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca
    38761 atacagttgg aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac
    38821 tatttttcta gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca
    38881 aataacagtg ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg
    38941 gccaacgatg atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat
    39001 ataagcagag aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat
    39061 acatgtcacc attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct
    39121 ttctggattt actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac
    39181 atccaaaatg aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg
    39241 aagatcttga tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat
    39301 cattgttgta aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa
    39361 atttgtcacg aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt
    39421 tctgcccttt tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag
    39481 aagtaaacaa aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg
    39541 ccatatcaat aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa
    39601 gcaaagcaat cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca
    39661 aagtttcctg agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact
    39721 ctgtttgtct catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt
    39781 gctgatatac ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga
    39841 tatgttctaa taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac
    39901 ctaaagaaaa ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga
    39961 cattttgtcc catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac
    40021 ggatttgttt catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta
    40081 atgttttcgc attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca
    40141 accgatccag tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg
    40201 cttgtttttt cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact
    40261 ctgcttctcg tagctgagtc tttttctcca attccttttg agtgagatag gcactataat
    40321 tccccgaaaa ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat
    40381 ctagaaaata gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt
    40441 caatcagtcg atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc
    40501 cttgttcata agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc
    40561 atttccgctc tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta
    40621 ggtcctttgc ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt
    40681 ggattttccc atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac
    40741 cgacaccatt ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa
    40801 tagatagttc tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat
    40861 tttccatatg ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga
    40921 gcagaaaaat ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa
    40981 ctatgactaa atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa
    41041 tggacatact ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt
    41101 tattcaaaag ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta
    41161 ttaggttacc acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt
    41221 ttattcgaac atagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac
    41281 cgcttttcct attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc
    41341 ctctgccaac tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg
    41401 cgcaactaac atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat
    41461 ttcttctctt gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac
    41521 ttcttttggc atcttccctg attcgtaagc ttggttgtag atttcttcgc catattcgta
    41581 acattgcata tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt
    41641 gaaacggatc aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg
    41701 ccatggtttc atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat
    41761 ttcttcgtgt gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat
    41821 aattgttgaa aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta
    41881 tattgcaaag ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat
    41941 cgctgaaagc cggggatagc acggaacctg tatctttatg gtccatttca caatgcttga
    42001 ttttcttctg ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc
    42061 tctgacgcga aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt
    42121 gatcaatctg caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc
    42181 tcatcatgat ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga
    42241 ttttttgatc ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa
    42301 tttcctcctt tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa
    42361 agaaaaaagt caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag
    42421 agaaaagaaa ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa
    42481 ccaagattat tgattcttat caaactatag aagatagctc ttctgcagta gataatttta
    42541 caccgccaaa aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg
    42601 atcagtatgc ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg
    42661 agtaaacggt gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg
    42721 aaaatctgtt tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct
    42781 ctttttaata accataaaat cctattcctt catatctcca gcccatcggt gttagccaat
    42841 ctctttcctt agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata
    42901 gtcgataaat tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga
    42961 tttttgttaa agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag
    43021 gattgtataa acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat
    43081 attcccagcc tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt
    43141 ctcctgtatg acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat
    43201 actgtgtcag attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag
    43261 tggcataacg acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc
    43321 atgcaccgga atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt
    43381 tatcttgcaa tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc
    43441 attgcccatt cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt
    43501 taatcccaaa tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt
    43561 caacgatggc ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt
    43621 gtgcagttga ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat
    43681 aaaaagcata gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca
    43741 atgtttgccc atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag
    43801 cttctgctga tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt
    43861 cggttgaaat tgtattttct gagacagtag ctgactgcaa agtactttct gtatcgtttt
    43921 caattcctgt ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag
    43981 ctagcactgt tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat
    44041 tctttgcttt catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa
    44101 ctaaaaaagt aaaagcaagt caatgtccat atttaaataa catttgtttc tttttaataa
    44161 atttgctttt tctgcataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta
    44221 aattgatggt cgtttgaata actatttaac aagtaattct ctctggatat tatccgttat
    44281 ggattatact tcctgtgtcg tcggacggat aatcatgttg ctgacagaca tccggtctgg
    44341 cgtttctatc gcaaatgcta cagcagaagc tatatcttca gatgttaatc cccattcttt
    44401 ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt
    44461 ttgaacagct cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg
    44521 taatccttcc atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg
    44581 ataaacaaca tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc
    44641 catgattgga agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat
    44701 attttgccat tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt
    44761 attgtacaat acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac
    44821 ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc
    44881 ttttattgct tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc
    44941 tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac
    45001 tttttctgat aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac
    45061 tcactatctc ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt
    45121 ggcagggatt cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc
    45181 tgcaacaatg gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat
    45241 ccaagcattt ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc
    45301 ggttagatgg ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa
    45361 acagatgcca cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat
    45421 actaatatgt ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac
    45481 tttgtcacta atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt
    45541 attcagcgct gctaaaggct cttcgttttc cgcttggatc tgatggatca cttcaaagag
    45601 cggatccgtt actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt
    45661 gctccctcct tttatcctta ctttataacg gacggcttat gatgtctaat acttataaaa
    45721 catacttaac tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga
    45781 aaaaatggat attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag
    45841 ccgagcagca aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt
    45901 agaagaagaa cttggtgtga cacttttcac tcgaggctcc cgagagatac agttgacgga
    45961 ttctggtcga tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc
    46021 gaacaattta acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac
    46081 taaagcaatc tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat
    46141 ccagtttcat ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt
    46201 acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact
    46261 gccagcccaa gatcgttggg gacttttgat acatcggaat catcctttag caaagcaaac
    46321 agtcatccat ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt
    46381 ggacagtcaa ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac
    46441 atataactta ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc
    46501 tctagatggg atcatcaata cttatgggac ggaaaaagta ttcatcccac tcgatccgcc
    46561 attacaagct tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg
    46621 taaattttta gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt
    46681 cagcttttta acaacgaata agttaagcaa aagatccaat gattaaataa cataagtttc
    46741 ttaatttgtt ccttcctttt atttctgata attccagtaa attactataa ttataaaaaa
    46801 agaattgaca aaacataact gtctatataa aattgattac gttaacaata attcgtttca
    46861 tgtgactaga tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt
    46921 tttcgtgtct atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc
    46981 ttcaaaaagg gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta
    47041 gatgacggtg gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga
    47101 aatgatttga tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa
    47161 atcaagttgc aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg
    47221 atcattgatc atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct
    47281 ttgaccgacc acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac
    47341 aaacttttaa gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca
    47401 gtagagtatc tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc
    47461 gcaatccata ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa
    47521 gtgacgattg tatctgacat catccattta gtggaatcag aattggaaac agatatccat
    47581 tcagaaccat tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa
    47641 cgaacccatg cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga
    47701 aaatacccag aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac
    47761 cagctcgcga tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga
    47821 agcgcaatcg tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc
    47881 tacaacgaat gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac
    47941 tagtagggat cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac
    48001 aagctggcgg taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac
    48061 tagggatgtc agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag
    48121 ttccagtaaa tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat
    48181 cagttgatcc atcttttgct cagatcagta acgtatttat tggtattatt gccggattga
    48241 ttgcggcagc tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct
    48301 ttagcggcaa gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag
    48361 tgctactgct cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt
    48421 tgagtttagg ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta
    48481 caggattgca tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata
    48541 ttggtaaatt tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat
    48601 ttttcccaat catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac
    48661 gtccagaaaa caaagcaaaa acagcttcct tgatgttagc agcagctttt gcttcattct
    48721 ttactggtgt aacagaacca cttgaattct catttatgtt cgttgcttgg ccattatatg
    48781 tcttgcatgc aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag
    48841 caggatttgc ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg
    48901 cgaatcagcc attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg
    48961 gctttaactt tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc
    49021 tttcagatga cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca
    49081 aaatctacgc tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac
    49141 gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg
    49201 gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag
    49261 tacaattcgt tgcagatgag atggcaaaac tacatggtgg tgctgcaaca cgacctgcac
    49321 aaacaaacac cgtggttaaa acagaaacat ttgccggtgg aacgatcgaa caagatattt
    49381 acgctatcgc aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg
    49441 aaaaaatgat gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa
    49501 tcgctggtac gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg
    49561 ctggtatcga agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat
    49621 tcactttata tgtaaaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg
    49681 atcttgccgc tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg
    49741 gcgacaaagt ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa
    49801 tcggctctgt ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa
    49861 caaacaggag ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt
    49921 ctcctgccaa ctccgcggta ataagtccag ccaaacgtaa catatgaaga gctatttttt
    49981 atttgactct tcttattact tgatgcagaa cagctttttt acaacctctg ataaacggtg
    50041 ttcaaaaacc agtttatcgg tatgaagtca aatttatgca aaaatgtgga aagccatttt
    50101 cacaaaattt ttcttcatac tcaaaactaa tcggcttttt cacaacctct ttatacatta
    50161 ttcctgatcc aatatttctt ttaatgctgc ttcaagagaa gttaatggtt ttcccaatac
    50221 tttttctaaa tcatcagagg aaatagctaa ctgatccttt ttaatatcat gttggatcgc
    50281 taaaaacatg tcagctgctt cttgagggaa tccggcttct tttagatttt cgacaaacgc
    50341 tgcatcactt gcttcaataa tttctaactt tttacctgtc gcacgctcta atgccttggc
    50401 taattcttca tagtcagtta gattgccgga taattctaag atttctggag agtcactttc
    50461 tactagtact tttgctgcag cttctgcata ttcacgtttc aatacccaac ctgtttttcc
    50521 atttcctgca gcataaacga attgaccagt ttttaaagcc tgcccaatga taggtaattc
    50581 attctctaca taccaattat tccgtaaaaa tgtatgggaa atacctgaat ccttaatcat
    50641 tttttcagtg aattggtgat ctggcgctaa tacactgatc gagtgatcgg catctgcaaa
    50701 acttgtataa gcgatataag ataccccagc tgcttgtgct gccttcacta cattcttatg
    50761 ttcggcttga cgatttcctg gaacgccaga gacaaaaagc aatcgatcta ttcctttcaa
    50821 cgcttctttc attgagtcta aatcatcata atcaccaata cgtatattaa atccagcttc
    50881 tttcagtgct tctccttttt cttcactacg tactagtaca tagatttcat tttgtggaac
    50941 catctttttt aagaattcca gcgcataacc accaaaacct ccagttgctc ctgttactaa
    51001 ataatgcata tttgccactc cctattcttt ttatttgtta agatttacag ctgtacattt
    51061 ataaattaca cctttatcat aaagagtgtg gtaagataag tcaagcaaaa aaaatgaaag
    51121 gatgacaact gatgaagtat tctctgcaat ttagtgatgc aatccatgta cttgcctata
    51181 ttgaaatgtt tcaagatacc cgatcacttt ctagtgagat gattgccagt agtatcgaaa
    51241 caaatgcagc aaatgtacgt aaaatcatga gtcagctcaa aaaatccggt ttgatcttta
    51301 caaaaaccgg aaaaccgcag ccttcccttg ccaaaagtcc agatcagatt tctttactag
    51361 atgtctatcg cagcatcgaa ggtaatacac agcttatcca agtcgatcca aaaacaaatc
    51421 caaattgcgt cgttggtgcg aatatccaag aagctttaga agaaagctac gctcggctgc
    51481 agaaaaaggc agaagaagaa atggctaaga tcacacttga tagtctgata cataaaatag
    51541 ccgttttgga aaaagaaaaa agaccagaaa acacaggcct cgtagagaga tttttataat
    51601 tctctctact gtgcctgcat ttttctgatc ggaatcgtct acataaattc ttttattctc
    51661 cgggggacaa aagccttgtc atgccccttt ttcctctcta atgctcaggt caaagcgctg
    51721 aagtcatgac gtcttgataa acggtgttca agtagctgac cctcggcatt agttctgaaa
    51781 aatgcaaaat atggaaagct atttcccatt tttcctctct aatgctcagg tcaaagcgct
    51841 actttcacga cctctatatt atcatggttt attgactacc ggtatccaga tttctcccat
    51901 atatttcccg ttggtaactc ccatttcgac tacagcattt ggacccccaa cataggcgta
    51961 atcttctact tctcttaaaa catgcccaaa ggccgtattc gtcaaatcat ttcttaattc
    52021 ttcaggcgat tctgctaaag cgattacaac aagatacgtt ccttttggga atgggatgat
    52081 tcgggtagtg tttggaacat cctgactcgt ttgaacaccg acatagtaca tcattttccc
    52141 gttatagggt tcgttgactg taaagaaata atgattttca gcagcttgtt tcaatgcatc
    52201 tattgttcca ttaccgataa ctttttgctc aaatgcttgt ttctctgcag ccaaaccgac
    52261 aaaatccgta ttgctactct tcaattccac accaataccc gtcaccgtaa aatctggttt
    52321 ttcttcgatc ctatactttg ccatttctcc caccctttct attatattaa gttcatctta
    52381 cataaaatta aagggaaatg acacgattac gctctttttt ctagggatat ttttattttt
    52441 taactatttt tttgctttca ctgcaatcca aatctcactg taaacattac tgaaatcctc
    52501 tccccatttt gtaaaagaga tctcgggagc ttcgactaac tcataatctg aagaaggaag
    52561 ccattctgca tagattttcc cccaagtatc ttgtaaagtt tggggaaatg gccctttatt
    52621 aggaaaaatg gcccaagtgt gttcttctat agcaagcatt tctaaatcag cgtacggatt
    52681 ttcttttgtc gtagcaaagc caatcatgtg catcaaactg cccttttctt ccaagcgggc
    52741 tccatcaaat ccataagaaa cgttgattac ttgatttgga tataaatctc ctaacttatg
    52801 catctcttcc ctttgagtct ccgtgatcgt ctgcgctaac ttttgtattt cttgattgac
    52861 tccttcaaat ttgacaggaa cttttttcgc cacacctgcc acataaaact gttctttctt
    52921 ttcgatacga aattccatcg attttcctcc tctgacatca ataaaaaatg tcaagcgtgg
    52981 aaaagatttt tgtatgccgt atctgctgat ttctgaaggc agatatcctg accaatcacg
    53041 aaaagctctt gaaaaacctt cgactgtttc atatccatat ctaaacgccg tttctgtcac
    53101 tcttgatcca tctaggagat cgcgattagc caaagccaat ctacggtttt tgatatagtc
    53161 cattagcgac atccctgcaa taaacgtaaa gatgcgtttt aattgatatt cagagattcc
    53221 tgtattttcg gctattattt tataggaaag tccttctgtc aaatgttctt cgatgtaatc
    53281 gatcaattgg ttcagatctt taagcatatt tatcttcctc cttgcttgcc tttttatcat
    53341 attatcaaag aaagtcaatt acttgatgaa tcaggaacca atttagtcag atgtagaaaa
    53401 tgttgtatgg atctaaagaa aattctcaag cagcgtttcg taacggataa ttggtattat
    53461 ctgcctatct atgttttacc tttagaaaaa taaattttgg ggaagacaca ttctaagtaa
    53521 acaaagcggt aaaaatatct tttattccct tagttaatcc gcttactacc tactgtacaa
    53581 agcaaattgt tgttcaaact gctgatcaga acgctgcttg gatttttctt tgcgatacat
    53641 catacctctt ttatcaattg gaacaattat ttatttctaa caaaatcact tcatttttgt
    53701 cagataaata aaaaacaaaa aaagttccat ctttaaaaca cgctattctt ttactttttc
    53761 tcacattctc taagaaaaga aaaggagaat gaatttttga caattcgagc aaaagtgatt
    53821 atcaaatatg taaaatcagc tacttttttc ttaaacgttt ctttatacta aatataaata
    53881 agtgtttcat gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt
    53941 ttggaacgcg gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc
    54001 aagtacttgt ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat
    54061 gaaaagacca acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac
    54121 atggctgctg tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt
    54181 tccgaactct cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca
    54241 gagattctta tagaacatcc gcaatttatc gaacgcttca aagttaagat gccatttcat
    54301 gcaataaatt attcagataa attaacaact atcatccata aatacgatca tattatcaag
    54361 aaaaacaaaa acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa
    54421 gcagttgaat cgctcattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa
    54481 gtgattgcta ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg
    54541 atagaggact gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa
    54601 aggcaaatgt tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa
    54661 cgctactcca taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag
    54721 aaaaaaatag ataaaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag
    54781 caagtaaacg ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa
    54841 acacgaatca tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt
    54901 tgactattag gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctgggta
    54961 gattttccgg cattgttgat agattttatg atacttttct acattttgta agagaggttc
    55021 cactgcatct gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccactc
    55081 taatctttaa agataaatat taaaataacg attaataata ttaacagtct ttatttttca
    55141 ccaccaaaaa cctatctttg catatactaa atagacgtat atacacagat aggtttgtct
    55201 tgtgaaaaat aatgattatc cttaccaaaa aatgtttgtt tttctacatg ttcgtactta
    55261 tcaaaaaata tgatccttat tgaaattact ggctatttat attccaacga cttcttgtgt
    55321 taatttttgt tatctcatag taaattatgt ttcattttca cttaatttag ttgtcacgtg
    55381 ttcgtcaatt cgtaaaaacg gtaaatatat aaacacactt gcggctataa ttaatatttg
    55441 taaaacagct gctcgccagt ctccggctgt tgctaaaaat cccgatataa ctggtggcgt
    55501 agtccaagga atttgcacta ctacatgatt aattaatccc acagcagttg caaaatatgc
    55561 tactgtcaaa gaaaaaatcg gtgcaagcac aaagggaata atcagactga tattaaatac
    55621 aataggcaat ccaaaaataa taggttcgct tatattgaat atagcgggag ttaccgataa
    55681 tttagcaaag tccttataat cttttctacg actgaagata aatattgcaa tcaataaagc
    55741 aatcgtattt cccgatccac ccattaccgc aaaggtatct ttaaaggcca tattgataat
    55801 atgtggaatt tgctctttgt ttgcataagc tgccatattt tcttgcatat tctgaatcaa
    55861 aaacggatct aacaatgaac ctgatattac tgcttgatgg ataccaaatc caaaaaatag
    55921 attagcaata gatgtaatta acaaaaatcc tggtaaacca gttgtaattt tacttaatgg
    55981 ctctgtaact atcgtggtaa ttagcttatt tatatccata ttaaacaatt gaatgattat
    56041 aaatgacaat aaagcaaacg aggtaatcgt aataatgatt gggattaata cattaaaaga
    56101 ctttaaaaca gcgggaggaa tatttccgga tagctgaatc tgtagtttct tattattaga
    56161 aagtttaata aataattctg tagccgctaa tccaacaaat atacctacaa acattcctga
    56221 agctcctgta taggatatag gtatcacacc cgtcacttca acagctttat ccgcattttc
    56281 aggagtaaac atcatctgca taggtgttaa tacaacaaat gtcgaaatag caactaatac
    56341 cgctgcaata tgatttgtat aatttctgct tgcacacata tgataagata ctgctgctac
    56401 aactaataat gtagttatgc ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa
    56461 cgtggtcaat gtactagtat cgacaatttc ccccatgaat cctgttggtt ctaagatcac
    56521 tgaattaata agtgtcacaa atcctgctaa catcataaat ggcataaatg tagcaaacgc
    56581 atctcgcaaa gttcttaaat ggatctgatt ccccaactta gtcgcaacta taccaaattt
    56641 ttcaaataca atgtccttcc tgctactttt gcccatattt tttctccttt atgcttattg
    56701 taatcgatta ctttttccac ccaaataata catcacttat caaaaaaagt aaatactttt
    56761 aattaaatat atatatcttt tttagtataa aaaaataagc tctgaaaaaa taattttccc
    56821 gagcttattt ttcaacaaaa acagcttcaa aaacaaagtc ttgatataaa tgtctcgata
    56881 ccgaatactc gaaaggttta ccagacgata gagcaacaac tttgtctatc tcaatcataa
    56941 attcacctgg atctattttt aatatttttg cgtcaaattc attcgcagta tctcctttga
    57001 tacgtatgtg cgaagtcttt atttccagac ccagttcatt tgtgatataa gcatatacag
    57061 atttctttaa atgttttggt tccaagcctg gtatgactgc taatggcatg aatgtttgct
    57121 caatcgagta tggttgacaa tcaataaaac ggcttcgtac aatattatat acatagtctt
    57181 ttacactgat acttaattta tcagctactt ttttcgatgg aagttcaatt gagaaagtat
    57241 ggatttctga atctacgtgt tcttcaccaa ccacgctaaa caaaccttcg tttggaccaa
    57301 gaactcgtga gttatcaatt ggtgttctga cgacttcagt cccataccca cttctacttc
    57361 gcaaaactcc ttcggaaaca agtaagtcta cgcctttctt tactgttaac ttgcttgtat
    57421 gataaataac tgccaattca tctcctgtag gaagtttctc accaatttta tactctccat
    57481 ttacaatttt tttctttata tctaagtaaa tttccatata cttcgcaatt tttttcaatg
    57541 gtgaccctcc aatcaaaaac atatcttcat ctatacttta acataaataa accgataaaa
    57601 tccacatcaa aattatacac acaataaaaa agatatatat atttaaacaa aactatttac
    57661 tttaagttgt aagcggtgtt atcattaagt ggaaacagga ggtaaaaaaa ttatgacaac
    57721 tatgaaaaag ctaacttttc cagatgattt ttggtggggt tccgcttggt cagctgagca
    57781 agcagaagga aaaggagaaa caggaaaagc agagactatc tgggatcgct ggtttaaaga
    57841 acaaccttac cggttttatc aacgaatcga ttcctctatt acaaccgatc acatccatca
    57901 atataaagaa gatatagaac taatgaaaaa aactgggcat aactcctttc gcgtttctat
    57961 ttcttgggca cgaatgtttc caaatggcgg taccggaaaa gtaaatccca aagccatttc
    58021 tttctatcga aaattatttt ccgaaatgaa taataacgga atcaaagtat ttgccaactt
    58081 atatcatttt gacatgcctg ctactcttca agataaagga ggatgggaat cacgtgaagt
    58141 agttgatgct tatgttcatt acgctgaaac ttgttttaaa gaatttggtg acttagtttg
    58201 tcattggttt accttcaatg aaccacttgg gcctatttta ggtacttatc ttgaagattt
    58261 tcattatcca aacctcattg actttaaacg cggagcgcaa gcagcattct tcactatact
    58321 tgcacacgca aaagcaatac aagtatttaa agaactagct cttcctagta aaataggcgt
    58381 tattctaaat ttgagtccta cttttcctcg tagtcaaaat cctgctgatg tgaaagcagc
    58441 agaaatcgct gatttatttt atacgcggag ctttttagat cctatggtta aagggaattt
    58501 ttctaaaaag cttatcctgt tattacaaga atataatcag cttcctgaag attatacgaa
    58561 agaagatctt gtatgtatct acgaaaatac cgctcaaatt cttggactaa actattacga
    58621 gccacgtcgc gtcaaatctc gttcttctat ggtggataca tctagtccat ttttaccaga
    58681 gtggtttttc gaaccacata tcatgcctgg acgacgaatg aacgaatacc ggggatggga
    58741 aatttacgaa aaaggaattt atgatttgtg catggatatc aagaaccatt atggaaacat
    58801 cgaagcattt atctcggaga acggaatggg cgttgcggat gaagaacgtt tcatggatga
    58861 tacagggcaa ataattgatg attatcggat tgagtatatc aaagatcatc ttgcttactt
    58921 atggaaggca ataaatgaag gatgtaacat taaggggtat catctttgga catttattga
    58981 ttgctggtca tggatcaacg cctataaaaa ccgatatggg ctggtttcac tcgaccttac
    59041 tactcaaaaa agaacaatta aaaaaagtgg cgaattttac aagcaattaa gtgatacaaa
    59101 tggatttaga tacgatgtag acttgttagt tgaataatat tctaaaattc cacttgaagt
    59161 gaacacatta gaaaacaaga ataatgagaa ataccaataa caaatacttt ttcttcatac
    59221 ttcatttata cttgtcagta taagagactg tgacaaatgt agtgatcctc agaagttagg
    59281 tctggaaatc taacttgaga agattttttt aaactacgtc cagtcttttt tatttgttta
    59341 aaattgaagt tgacaagtct tcattaaaaa tttagaatat tcacaagttt gacgacaatt
    59401 atgaaaattt atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa
    59461 ctgcgatcaa agacacgtta cctaccgttt ttggttatat cggtattgga cttgcatttg
    59521 gtatcgttgg gaaagctgcc gggtttcatc cgctggtcgt cacgttgatg tccctgctga
    59581 tctatgctgg ttctgcccaa tttatcacgg tcagcatgct tgctagccat agtccactgc
    59641 tctccatcgt tctctcgacc tttctagtca attcccgaat gattctgatg agtatgacga
    59701 ttgctcctta tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga
    59761 cagatgaaag tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact
    59821 ttcgttggat gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg
    59881 ttggcgcact tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca
    59941 tcgtcgctat gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta
    60001 aacgattaca gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt
    60061 ttatcccaag taacttagtg attttagttg taacactgat tgggtgcgga ttgggggtgt
    60121 ggatcaaaca tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca
    60181 acttggcttt ctaggatcct accatttgtc ttactgaaaa aattcgattt gccgcaaccg
    60241 ttgcttgagt atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt
    60301 ctatttaccc aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca
    60361 gttcctacac ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggtagga
    60421 attctttccc tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat
    60481 tactgtcatg cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg
    60541 cttatccgtt gaacggacca ctaacaacga cttttcctaa tatatgtcct gtttctacgt
    60601 ctttggttgc ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg
    60661 tactgcggat aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg
    60721 tttcgatttc atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag
    60781 attggttttt ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat
    60841 gtccaaatgg tcggacaagg tgtttgattt gatcaaagta gcgggtaata tcgaataata
    60901 cagcgacata ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat
    60961 gataatcgat tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg
    61021 cagtcgcgta gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc
    61081 ccccagctcc gttgatcact aagatttttt tcccgcggtt ggcattttct tctggagtca
    61141 gaccaaattt ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcgg
    61201 cttcttcatc tgaaagattt tttggtgcaa gagcgacaat acgttcgtca accagttggt
    61261 attcttgatt gcttcctgct cttgtcgttg tacctgcgta aaaaacgcga tcgccgacag
    61321 aaaatttttg cacttcatca cctacagcaa caactttccc gactccatca aaccctaaga
    61381 tacgtaaagc atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag
    61441 atactgcgtt gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt
    61501 catcaataaa gctttttgga tcatcgatcg acaacccttc ataaaaaccg actaccttca
    61561 ttaattcctt ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac
    61621 agttttcatt tcaatatata tagtatgcta tttgttccac ttttattcat cataattatt
    61681 ctcttactta aagtaaacta tttgcacttt tttggttaaa aaagaggttg cgacaagact
    61741 tttgtcacaa cctcttttaa acggtgttca atcaactgac cctcggtatt cgctcaaaaa
    61801 acgacaaaat atgaggagct atttttgttt ggctgttctt attacttgat gcaggacagc
    61861 tttttcacga cctctaattc acggtgttca agcaagaaat cattgttcac tgaaaagcgt
    61921 tggatgttct ttttcaaagg tgtctaagat ctctcgtact tcatcagtag atttcgtatt
    61981 catcaactga actcgcaagt catttgctcc tggaaatcct ttgatataga ttttaaagaa
    62041 acgatgtagt cctgtgatgg aacgcgggat gatttctgaa taatgatctt gcagatctag
    62101 ttgcagacga agtagtccga tcagttcttt ttctgtatgt tccctcggtt ctttttcaaa
    62161 tgcataagga ttcttgaaaa tcccccggcc aatcatgatg ccatctacgc catatttttc
    62221 ggccaattct tctcctgtct ggcgatctgg gatatctccg ttgatcgtga tcagtgtttg
    62281 cggagcgatt tggtcacgta aagcaatgat tttagggatg acttcccaat gtgcatctac
    62341 tttactcatc tctttacgcg tacgcaaatg gacagaaaga ttggctatat cttgatgaag
    62401 taggtgactg atccattctt ccatctcaga catttccgta tagccgatac gcgtcttgac
    62461 gctgacaggc aaaccgccag ctttagccgc ttcaatcaat tgtgccgcta cttctgggcg
    62521 aaggatcaag ccactgcctt ttccacgttc tgctacatta gggactgggc agcccatgtt
    62581 gatatcgatt ccttggaagc ccatttctgc catagcaatg ctcatttctc taaaaaattc
    62641 tggcttgtct ccccaaatat gggcaaccat cggctgttca tcttctgtaa aggtcaatct
    62701 tcctcgtaca ctgtcttttc catctggatg acagaaactg tctgagtttg taaactcagt
    62761 aaagaagaca tccggtgctg ctgcatgctt tacgacatgg cgaaatacaa catctgttac
    62821 atcttccatt ggtgctaaaa caaaaaaagg ctttggcagc tctttccaaa aattagtagt
    62881 catttatttg ttactctcca ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat
    62941 tgtcattata ctaggaaaca aaaaaagaag caaagtgaac agcttaaaag atgtttctga
    63001 ttgagcaaaa aaagactgtg acaaaaatct tatcacaatt ccttttccgg aataaatggt
    63061 gttcaaaagc ttgcatctgc ggtaataagt ccaaccaaac gaaaaatagg aggagctatt
    63121 tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct ctccactaaa
    63181 cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cgacaaaata tgaggaacta
    63241 ttttcccgtt tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttttcta
    63301 acttctcaat cctcttcctt tattgatcaa ataccaagta acggcgtaca aaacaacgat
    63361 gaatagcaca agtacgatca tcgatgttgc tactggcaca tcgattgttc caaggaatcc
    63421 gtaacggaaa ccggagatca tataaacgat cggattgact ttcgaaacag cttgccaaaa
    63481 tggcggcaac atagagatgg catagaaaac gccacctaga taagttaacg gctgcaagac
    63541 aaatgtaggc acgatcgata catcgtcata agactgagca aaaatcccgt tgagcagtcc
    63601 tgctaacgaa aacaagattg ccgtcatcaa taatgtaatg atgaccatga accatgaata
    63661 cacatgaagt ggtacaaaga acaaggaaat gatcgttacg agagagccga cgagaatact
    63721 gcgacccact ccgccaatca caaagcccca aatgatcaca tgcgtaggaa caggcgctac
    63781 caagatttct tcgatgtttt tctggaattt ttgagagaag aaggacgaag aaacgttggc
    63841 atacgaacta gtgatagcag acatcataat caaaccaggc acgatgaatt ccatatatga
    63901 aaaaccaccc atgtcaccga tccgaccacc gatcattttt ccgaaaataa caaagtacaa
    63961 ggatgtcgtg atcacaggcg gaaccagtgt ttggacccag atacgcaaat agcggttcgt
    64021 ttccttgata gctaaacttt ttaatgcagt aaaataaaga ttaaacattt ttctcctccg
    64081 tatgattatt ttcttctgtg attttcaaaa acagttcttc taaacgattc gacttattcc
    64141 tcatagaaag aactttgatt ccttgtcttg tcagctgatc gaagattccg ttcacccctt
    64201 gatttcgttc aacttcgaca gcgattgtct gttcatcttc aaatgtacaa gcgtatcctt
    64261 caatcacagg tttggtctca tatggcgcta aatcaaagat aaatgtttca aattgcagtt
    64321 tggatagaag ctctttcata ctagtattct cgatcagttc acctgattgg atgatcccga
    64381 tgttgcgaca aagcatttca gcttcttcta aataatgcgt cgtcaaaatg atcgttgtac
    64441 cattttcgtt caattctcgg agaaactccc acatctcgcg tctcaattca atatcgactc
    64501 ctgctgttgg ttcatccaag atcagcaaac gtggttcatg catcaatgcc ctagcaatca
    64561 tcagtcttcg cttcattccc ccggaaagca tacgtgcacg gacatttctc ttttcccaaa
    64621 gattggactg cttcaaatat ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt
    64681 agtagcctgc ttgattcacg acgatttgct ggaccgtttc aaagggattg aaattgaact
    64741 cttgcgggac taatccaatc tgttgctttg ctcggactag atccgtatcc agatcataac
    64801 cgaagacatt cacttttcca gatgttttat tgactaaaga agtaatgatt ccgatcgttg
    64861 tagacttccc agctccattt ggtcctaata gcgcatagaa atctccttct ttcacggaaa
    64921 gatcgattcc tcgcaatgct tcgaccccac ttccataaac ctttttcaaa ttatttattt
    64981 caagcgcatt tgccatttat ttcctatctc cttatagaca tgattcttta ttgtatcatg
    65041 gaatacagca gatgaaaaaa caacttggtt ccagcgaaaa atcaggttgc tgggctcagg
    65101 aaaatctcct aatgaaagac atcaatcgga cgaagtcaga caaccttttc agaaaaaaca
    65161 aatcgaagat aaaggattac tattcatatg tgcaacaaaa ggagcgtgag acaaaagtaa
    65221 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcaaaa gccaagattt
    65281 ttgatccaat aaatactttc gatcacttcg cctatcccaa tacatgccac tgccgctttt
    65341 catcaaataa cgcggcttcc gtttttgcgg tttctactaa agtctctggg taatccaaaa
    65401 ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa
    65461 aactgattcc ttttccttct gttacttgtt cttcaaagtg gacattctca caataattct
    65521 ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat ggatactccg
    65581 caagccaccg aacgactgaa gcggaagaag caatccgttc gatcgtattt gtccctttta
    65641 aaatctcatc tacgaagcag tagcagcgtt ctttggaagc cacttggtcc aacaaacgtt
    65701 tgatcgattt gatttctgaa acaaaataac tgtcgccttc aaataaatca tcttcgattg
    65761 ccatggaagt caacacatgt ccaggttgca gggtaaactg ttctgcaatc gctgtttgga
    65821 tcgtctgtgc tagaatacag ctgatggcta tacttttgac atacgtagat tttcctgatg
    65881 cattggaacc cgtgactaat gtattcttct gccagttgac cggattcact accgcttctt
    65941 ttaacaatgg atgatagata tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc
    66001 aagtgatggg catataagta cggtaattca atattgcagc ggctacttct aattctccca
    66061 gcaattccca cagcttgatg gcttcagttg aatgcttttc taatttagtg agaacgaaat
    66121 gataggagat aaatggcagc atgaacatga tgttgacata ttcaaaaatc aactctgctt
    66181 cactcccact cttcatacga aatgaaaaac ctaatttagg gatgtctctt aatggtttag
    66241 tttgctgttt cagctcttct tgaagtggtg tcggcagttt tgctagctga ttggcggctg
    66301 aaatcgttga aaccaaataa ctcatgctat tcagctctgt ttctagtgtt tgtttcttca
    66361 ctagataata gaccgtattg aatactaacc caccgattgc caaatagatt cctagtactt
    66421 gtcctaaaag aagcaaaccc aaacccacca gcggaagcaa gccaagaaaa ataaataaac
    66481 ctacgcgtcc aatcttttta ctttttccat ccgctagata ttgttttgca aaattatgat
    66541 ctttttttcc taaacgagcg aattgatatt ggattttttc tctcagttga ggattttcct
    66601 gataaaaagc aatcagtttt tctaactgct gatcttcacc aaaatcaaag ctgcgtaaac
    66661 gttgatacaa cgcttctgaa cctacactag aataggtact gttgattcct tcgaatactt
    66721 cgaacatatc caggtcatac caagtaagat catcgatttc gctatcccat gaacggaact
    66781 tcttctccgt gagccatgct tccttcaagc tttcttcttt atcaaatctc ggttgataag
    66841 gaattttttc ccatttgcta cgaactgttt cctttaattt caaacgtgaa aaaatgtcta
    66901 aagcaactaa taaaataaca gctgcaataa taattaaaac gataaattct ctttgaccca
    66961 tatccctctc cgttccttta tttgttagct ttagtatagc actgttgcgc tatttgaaaa
    67021 gagggtaaaa aaacaccttt caaaataaaa ggtgtttttt tcttgtatat tacatattta
    67081 tctctcattt atcaaaattg acaaataatc atagggagga attgttgtta attcttgttt
    67141 attctccagt gcattgacaa ctgtcccaag ggaaaggata gcctcaggta ctattttttc
    67201 ttgttctgtt ggattagcta agcgaagaac aaatccttgt tcatgaaaat aagaaggata
    67261 aatcgatgat acaattaagt cttcaggtaa ttcaatcaat gaaaatttac gaggaacatc
    67321 aagttcattt tgtaatggcc aaatcttgtt atctaacctg tgaataaata cattcagttt
    67381 ctgcttttga taagaaatag attgttctaa ccgtttttct gcctgttttg ccactttgaa
    67441 ctcattaaat cttccttctt ctacattaaa tccaaaagtg actttatact cacctatctc
    67501 ttgagctagc ggtgtttcca tcatgacatg cccttcattt gtcgtatctc ctgaagctcg
    67561 cccaggtcgc caagcaagat taggttttcc aagttcccct gttgttgaaa ataaggttat
    67621 gattaattga ttttctatac gttcatactc ttttaaacca tctgcataaa acgtaaaata
    67681 atgatcattt tctgccacag agacactctt atcgaatatt tctaaattaa caggtttctc
    67741 taccatagtt tctgaccaat tcggaggaat gacatcaggg gtattttcaa taaatccgtt
    67801 ttgaatttgt gcaattgatt tttcagattt atctaaaaca ttaattgcta agcgcatacg
    67861 atgcgagtaa acagtattat gaattttgag tgtgccttca acgatttgac tttctcccag
    67921 actgaacgat atttcataag atatttcttc catttcgggt ttttcagata aacgatcttt
    67981 taaatcttta ggaagttgag cttttccgta aacaactaat gtttcttgta aagaatcttt
    68041 atatacttta gctgtttcta attttagaga tagttctgta tctccttcta atggtgaata
    68101 atcatacgta tcgccatcat ttgctgaatc gatcaaatga acgaaatcaa gatattgtct
    68161 atctccaact atcaaattca attgcccatc tttaaaagaa agtttatatt tattgttact
    68221 tatctcagtt ttgtctatct cgattgctgt ttctaatttt tcattatttt tctcaaattc
    68281 aacaacagtg tatcctagtg ctggcacatt tacatttatt tttatgtcaa gttcataata
    68341 agctggttca tcaaaatatt cgaaacctgt agcaatcatc cgataagcat ttttccgttc
    68401 tggaaaataa acttcttttt caatgatatt atagggacta cctttgaagt gtattttctt
    68461 tgaatcagct actacatgca cgattttcga tccactataa ttctcagtcg ttgtgttgaa
    68521 aacaaggact tcattcgatt ttagctttaa tgcatcagcc atccgtttta caataaggtt
    68581 ttcgattccg tcagctatct cattagcttc tttgattcga tggaaaatat cttccgcaac
    68641 attatctgaa acacagccac caatactatc atgagcctgg ttttgcaaaa tctttttcca
    68701 taaccgacga agtaggcctt tactgatatg gataccattt ctttctgcaa tcagcatgag
    68761 cggctcaact cgttttagaa ttttttgttc caatttaaaa ttttctattt tcatttttgt
    68821 tcttactgag ccaatagaac gatgaacacg agagtaaacc ggctgacgaa gttcaccttt
    68881 atattcaggt aattcttttt tgtttctgat aacttctaca taagaaggat aatctgaaat
    68941 aatattgtgg aatttactgt atttattcaa cttctctacg acttgatcaa aatttaaaat
    69001 cattgacttt tgatctacac ccgaaggtac taaaatatct ttgttttttc cttgcatatg
    69061 aagcatttca actgcttcat ccaacttttc ttttgcaaat tcaggaatat ctttaattgc
    69121 gtctaaggtc atcattccag tcatataacc aaatggaaaa ttcatggcat aaacaaattt
    69181 atctcccaat cctttccaga taaaataagg agatggaacc atcgtttcaa agtttaatcc
    69241 tcgccaacac atgaaattat cgatttctac ttgatgcaaa agtgtaggca aattcgcatt
    69301 gaacccaaac gtgtcaggca aataccctac catcattgga tctccatatt taaggatcgt
    69361 atcactgatt ccaatcatta agtttcttaa aattgattca gcatccacta ataaggcatc
    69421 tgtttgtgca taccacggtc ccacgaatag ttgtcctgtt ttcatcagtc gttggataac
    69481 cttaagcttt tgcggattga tctccaaata ctcatcgacg attgaagttt gtccatctaa
    69541 acaaaagttt actttagggt tcctttccaa ttcatctaga acttcagtaa agacctgatc
    69601 agacaatacg atggcatctt catttgtaaa ataccattct ctgtcccagt gcgtgtgttg
    69661 aattatatgt gcatttacca tatcagattc tcctactttc cttcttctac tgaatttact
    69721 tgttgttctt ttttatactt gatggatttt agtgcattga ccattaacgc tgtaaccact
    69781 cccccaataa caattgatac taaatataaa ggtatattcg taacaaaagg aattgccata
    69841 aaggttgaaa gcattgtttc ttgagtaaca ccaaaaaatc catttagacc acatgctact
    69901 gcagtgccca ccacgatgct tggtattact cgtaaaggat ctttcacggc aaacgggatc
    69961 gtcccttcag taatccccac aaggcccata atcatagctg tctttgcatt atctcgttcc
    70021 gattcatcat aaagtttttt gtttaaaatt gttgctaaaa acattccaaa tggcggaata
    70081 gcaattccta ataacatggc tgtactaggt ccaaagcttc cgcttgaaaa agaagcaacc
    70141 ccaaaagcat atgcgatttt attgataggt cctcccatat caaacgccat cattcctcct
    70201 aaaatagccg acatcaaagc agcatttgct cctgacatgc cttctagcca attagtcagt
    70261 cctgttgtca ttgcttgaat aggcgtagct agtacatacc ataatatgat tccaacaact
    70321 aatgtagtaa caaaaggaat cagcataatt gattttagac taataacttg catcggtact
    70381 tttacttttt tcaatgcatt agctaaatag ccacaagcta atccagcaac cattcctcca
    70441 ataaatccca taccaagatt ttgacacatc atcccagtga taaaagctgg tgctagaccg
    70501 ggacggtcag caattgaata tgcgatatag gctgaaatca tcggtacgat aaactgtaat
    70561 cccgtctgac caatcattcg caggactccc cagaatgtat ccgtctggtc ccaaacacct
    70621 gtgccaccaa aaatgactgc aatagaagtt agtaaccctg ctgctacgac aagcgggatc
    70681 atataggaaa ccccactttg gaaatgattt agaatttctc ttccttgttt cttgaattcc
    70741 attagtaact cccccacgat atatttttta tttgttaatt aatgcaattg cttcatcaat
    70801 cgtctcttca ccatctttta ctacctctga ggtccctact gttaaaatcg gtttaccgtc
    70861 aaagcgacct cgttctgaaa catttgtatc tactgcaaat atgacaacat ctgcctcgga
    70921 aacatccgaa attgaaagtt tgttttctat ccccatagcc ccctgtgttt ctactttaat
    70981 atctaaccct ttcttcttcg caaacttttc taaattagct tttgccatat aagtatgggc
    71041 aatccctgcg atacatgctg ttactgctac tatttttgtc atatgaaatt cctcctttta
    71101 aaatgataga gcttttacca attctttttt atttctcgaa tcttttattc ttttaacaaa
    71161 ctcgtcatcc atcaatcttc cagataattc tgctaaaata cgtaaatgat tagtaccttt
    71221 atcagtgtct tttattgcta acaaaaagac gagatcaact ggttgatcat ctaatgcgcc
    71281 ccattcaaca gaccgcttta acttggctac agccactgtt gattcaatta ctgctaggct
    71341 tttgccatga ggaatggcga tatttttccc aattcctgtg gtcatatggt tttctctgtc
    71401 aaatacgtct ttttgaaatt gtttgtggtc agacacaaac ccattagtct ctaacaattt
    71461 cgataatttt tcgactacac tttttttatc agatgcattt aaatctatca ctatattatt
    71521 tatattgatt ttttcactga tcatttataa gccttctttc aatatataaa ctaatttttc
    71581 aaacgacgaa ctcgttttta aatttgctaa taactcatga ttcgaagcga tttcattgaa
    71641 tgtttcataa attttattca attcaggttt tactttttca ttaagtgcaa aaaagaaaac
    71701 agaatgaaca agtgtcccat cccaatcaat tccttctgga ttaacgtaga cacagattac
    71761 tggctttttt ataaaatcta cttcgatatg cggcatagca aaatcattca tcgctgttga
    71821 agatagtttt tctcttttga ttgctccctc tagtatccct tctttcgcgt aattttcttt
    71881 taccaatttt ttggtaatta tatttaaaac gttcacttga ttatccttta tttggtgggt
    71941 aaaaaagcaa aatcgtttat ctaataaatt tagaaaagca tcttctgttt gttcatttaa
    72001 ctgtagatac tgttttactt ttctgacatc tgcccctaac atcaaaggac taacaacaat
    72061 aacaggaaaa tttgtatttt caatcggaat cgtgcttata acaagttcat cagtgacttt
    72121 tctatcttgt agttcattga tgcttagtac atcttctatc tcaaccatat ctgcaaaacc
    72181 gttatttatt ctttgctcta ataattttga tgttccatat ccgctactac aaactaatat
    72241 cactttcgtt ttgtcaggtt tgtaacgatc caaaagtgat tgaatatgga gtgttacgta
    72301 agcgatttca tcttcattaa aatgtataca gaatctctct tcgatttcct ctaataaatc
    72361 tacacttatc ataaatgacc ttttaaaaga atatttgatt ttgtccgtat aagggttata
    72421 gatatttact cccaatttga gtcttttaac tgctgcattg agatgtaata ctagattatt
    72481 gatcaattct tcatcaggat aaaattcatt taatttaatt cttactaact cttgtagctc
    72541 aacacccata tctaaatagg aaagcttcgt ttgatctaag tcgtgattca tagcattttt
    72601 ttcaatcgaa gtaatgtgaa tgtcaacata agcttgttca tctattggca caataattga
    72661 aaatgctttt tcaatcattt tgattaaaat aagtgtattc tttgagattt cttcttcaga
    72721 attattacta cgtgataaga aattgcttgt ttcaatcctt tctatcgcaa tagcaaggtg
    72781 gatagctaaa gtttgaaatt cataatctgt aaatttcaat tgtgtcttat tcatgtatcg
    72841 attaagcaat tcggctactt ttttcaatat gtccttattt aagaaattat tcgcttcacc
    72901 aatgaaattg atattcaagt ctaagagatc tgttttactg ttagatgctg taaagccacc
    72961 ccaataatag ttcacaactt ctgacatcaa ccttctttta cttttttcat cagctttgat
    73021 gcgcacgcca tttcgatctg aaactaagga catcccatgc ttgtttagtc tttttctaac
    73081 atctttcatt gtgctttcta ccgtcgacct gctcacgtat aattcgtcag caagattttg
    73141 aatctttaga gaatcattag tattcaataa tttagcaaac acataagtta gacggtcctc
    73201 cggagagtct gcgatattga ttttatgact ggataataaa tttcgaagtg tttctttttt
    73261 tccaataagc gaaacgcctt ttttaggttg aaccaacatt tgtacatctg gcattttttg
    73321 gattatttct ttcaaaatta aaatatcttt gctaattgtt ttcgtagata catataattg
    73381 ttctgataat tctttgtaag ttgtgtgctc attttcaatc aaaaagtata gtattttcat
    73441 ttgccgttcg ctaaattcga tgcccattat cttcacctcc tgaatacagc ttaagtgtac
    73501 aattattatc tctattttca tacagaagaa agttcttgat taatgtagaa gtttctaata
    73561 ttcttgatta tttcaaatca ataaaaaagc ctgcaatatt tatatcacag gcttttttct
    73621 ctattaaata tatataagat aatacatttt tctactagtt ctcactaatc ttgtataatt
    73681 aaaccctccg caacttgacg gagatggaga agatgccccc gtctaatcta gcggtcatct
    73741 ctcctcccat tccttccacc aatttttttg acaggtaaag cccgattccg ctagattccg
    73801 tatttgtgcg gctggtattt tcggtataaa aacgctggaa gactttttca acagaggttt
    73861 gcgttcctgg tttgatagga ttggccactg ttaatcgaat gaatggatcc tcttttgaaa
    73921 atgtcacctt tgcttgtcca tctgcataac gcaataaatt ctgtatgata ttttgcagca
    73981 ctcgcagcaa catgagcgga tctgctaata cagttgtatt tatcgagtgt tcagcgaacg
    74041 ctacctcgat atttttctca gaaaattgtt cataaaaaga gaaaaatact tcttccacct
    74101 ctttagaaaa agaaagcgta tggagatcca tttctttttg cttcgtctct attcgagcaa
    74161 gatcataaaa ctgatccgtc atactgagta atcttgcaac agattgtttg atgatcgtta
    74221 aatacttctc ccgtttctcc gtatcctcta cctgttccag taattgtaca taaccgttaa
    74281 tagcagtcaa aggagttctg agatcatgag aaaggccgac gatcgatagt ctgatgtttt
    74341 cttccatttc ttgaaatgct tgtccttttt gttcattttc atccaaccat gtattcagtg
    74401 catctactag gtccattaga ttcttttctc gaaattcgac tgaaagacga ccaccatatt
    74461 cttgtcgttc agaaagagtg gcgattttct tagttaatct cttgatttcc ctttgcagga
    74521 agaaaataaa acttcccata aagataacaa cgagaagcaa cagccattta atcaaatcta
    74581 aaccccactc cccacaccgt atgaatatac acagcatgtg cctttgctag cttccctcga
    74641 agattggaaa tatgcatatt cactgttttc tcactatcaa aatatggttc ttgccaaact
    74701 gtttcataaa gatttgctcg cgaaaatact tttttcggat gttctaaaaa aagcttcagc
    74761 agatcgaatt ctcttcctgt caaatggaca gccacaccat tcacatttac atccctcgac
    74821 tctttgtcca gtaagatttc cttgtaatag atgcgttgcg acagatcttt tacttggaca
    74881 gaagtctgat gacgaagttg gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt
    74941 ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt
    75001 tttgctgaaa gaacgatgat cggaacattc gatgtctgtc ggatttctgc taacaatgct
    75061 tcacctgaaa gccctggaag catgagatct agaaggatca aatcgacagg atatgtgctt
    75121 aacaataatt ttccttccgt acctgaataa gcatcaagaa gtttgtatct ctgttcaaga
    75181 acctcctcca gtaaggaatg aacagcttca tcgtcttcga taatcaaaat agtttccagc
    75241 aggaccacct acttaatgtc agatttttgg aaaataaaca ttcctagagc tgatgatacc
    75301 acgattgtta ctagactgac cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc
    75361 gcaaagtccg tatacatgag ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct
    75421 ttgatttttg cgaaaacacc gggaatgaag gccgttccca tgatataaag aaaagaaaga
    75481 agcaaactga cactggtact tctagtgatg aatgtcaaag ccactggaac agataatgtc
    75541 cctaatgtca gcagaagcaa tacaggaaat acttttagaa cataatttcc atagtttttg
    75601 attccctcaa cttcaccaaa tccgaaagca agtgtgtaag caagcatggc tagtcccgtg
    75661 atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga cacataataa
    75721 gtcattcgat tccgtcctaa agataacgta ttgcgaatcg taccgttttg aaattcttcc
    75781 ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc cattttttct
    75841 accattaaaa tccctgttgc accgttgatg actgtatccg ttgcttggtt taattcattg
    75901 ccgtattttg tatgcatata ccattcgttc caagaataaa aaagcggaag tacgaccatc
    75961 agtcctagtg agacccacat tttacggctc ttccatactt ttattttttc agcagttaat
    76021 agattaaaca tgtggattgc ctccagttaa ttgtaagaaa taatcttcca gtttttgccc
    76081 aactaggtga ataccatcca caggaacttt tgcttctacc aatgcttgat tgatggctgc
    76141 tacttggtca attccttcaa aaagctggat aacacggcta gattgtacaa aataatccct
    76201 ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt caatttttat
    76261 aaattgacga gattcttgtg ccaattcttc tttcgataat tctttgatca agtgtccttc
    76321 atgcaaaata ccatagtgag tggcgatctg tgacaattca tctagcaagt ggctagaaag
    76381 caagacggtg atgccccgtt cttttaccaa acgttggatg atttctcgca tttctgccat
    76441 ccctgctgga tccatcccat tcgtcggttc atccaaaatc aaaaattctg gattcgtgat
    76501 cagtgcattt gcaatagcta aacgctggcg catacctaaa gagaaattct ttgctttctt
    76561 tttccctgta ttttccaatc gcatcatttt cagcaggtct tcgatttctc tatccgacac
    76621 accgccgttc gctgcttgga tacgtaggtt atctcttgca gttaattctg gataaagagc
    76681 aggggtttca atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa
    76741 taattcgatc gaaccttcat caatcgagat caaacccata atagttctca taaaggtaga
    76801 cttacccgct ccattttccc caatcaatcc atagatcatt ccacgtttga tttcgattga
    76861 tactttatct aatgctttgg cagcaccgta ttttttggtt ataccagttg ctttcaagac
    76921 cgtttcattt ccaaaatcca tacttatcct tctttcttat tgatgagtga agtatagttg
    76981 acgggtataa agaaagcagc cagaaatgta taaagaaagt gtaaattttc caattgaatg
    77041 tcaaaaaaaa caatcggaca atacgtgtct ttcctattat caggcagttt gttatacgct
    77101 tgtatcagtt tcctgattct ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac
    77161 tgaaaatttt tgcttctttc agatcaagct tgagtaattt taccagaccg gttataagga
    77221 taactatagc aagactccct cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg
    77281 tcttccaatc aaagagcgca ccaaacagtg ctaatccaag tggactggcc accatggtaa
    77341 tcgttgttat cgttgacata agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc
    77401 agaccatata aaaagcaaaa gaaaacatta atccttaaaa acggacttat gaaaaataat
    77461 acatgccgtt tgggatttac aattagctac caaataagag gttatgacaa aagacttttc
    77521 acaatccctt cttccgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
    77581 tatttatcaa acatgagaag caatttttgt aaattccttc ttatttcttg aagctgacca
    77641 cttctgtcac aacctcagaa gcggataaat aatattgttt gatgatactt tatctttttc
    77701 ccgtttgttt atagatcgaa tgatattata gaccaagaat cctacctatt aacatagcta
    77761 aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt
    77821 aactgcgctc agcattgctt cgcccagtac gattctcgta actcgtgaac tcgaacgata
    77881 atcgcaatta acgttttgag aactgtgaag ctttacgggc tttcttaaga cctggttttt
    77941 tcttcgtatc attgaataag tttgaaaaac cttgatttat aaaggttttc ccgtaaatat
    78001 attttattat atttgattaa atttgaaatg tttgtgttct aaaatgtgtt ctaatttaaa
    78061 ggcagtatat ttcgtatttt agattaaatt tttaggctca ttcattctca aatccattct
    78121 attcagtcaa taaaaatttt aaaaagactt tataaacaaa gatgttcaag cgagcatctt
    78181 atttttttgt gcttaatttg aaaggagaac atttcatgta taaaaaacaa aaaaagaaat
    78241 tatgtgtatt aactattttg agtttggctg ttcaagcaat tgttggagca attgctccta
    78301 cgattacttt tgctgatgaa attacccatc cacaaacggt aaccgtagaa ctagatttag
    78361 cacatcaata tgctgttgaa ggaactttta gtgatggtcg tccaatgtca gaagtcactg
    78421 ttccgcatta tgcagtgtac aacggcttta aacaagatat tttctgtatt gaaccaggag
    78481 tcccgattta caatgagttc actcctggct atgagaaaaa cccgttacca gatatgtccg
    78541 ataaagccaa attagtttcc gttttatgga aaaaagtagg tacagatgca gatactcata
    78601 ttgtggcaca gaagatgatc tggcaagaag ttaatgggta tacccttcac tcaataaaac
    78661 gatcagatgg tagtgccgta aatatcgcag ccattgaggc aaaaataaat caagccattg
    78721 acgattatca gaagaaacca accttccata atagtacggc taaaacagta ttagggcaat
    78781 cgactactgt gacggatacg aataatctga atttgtcaga gttcgatgag gttgtggaaa
    78841 atgccgcaaa cattgattat cgtgtgaatg gtaatcagtt gataatcacc ccaaatgcca
    78901 attcaaaaga aagtggtgtg ctgacgctta agaaatctgc tggtaccgga acacctgtcg
    78961 cctataaaaa ggctggccaa cagactttaa tggccggggc gattgataaa cctaatacct
    79021 acacaatcaa aattgatgtg gaaacggagg gttctttaaa gatcaaaaaa gtcgataaag
    79081 aatcgggtga tgttgtacca ggaacggttt tccatttaga ctttggaaaa actttacctg
    79141 caaaagacgt gactactgac aaagaaggca ttgctacatt ggatgagatt cctcatggta
    79201 caaaagtaac cattaccgaa aaatcagtac cggcgcctta tacgattgat accaccccca
    79261 tgactaccac cattaaagca ggcgaaacca tttacgtgac ttcaaaaaac gcacgggaaa
    79321 aagggcaaat cattctggat aaaagcggag ttgaaacagg gagtgatctt tggaatgaca
    79381 attattcctt agctggtaat acgtttgcca ttcgcaaaga cagtccaacc ggtgaaatcg
    79441 ttcaagaaat gaccacggat gaaaacggtc atgcggaaac accaaaagag attgctaatg
    79501 cgttggaatt gggaacctat tatgtgaccg aaactaaagc tagtcatggg ttcgtgaata
    79561 cgttcaaacc tgtaaaagtc gaattgaagt atgccaatca aaccgtagcc cctgttacca
    79621 gtaacgtaaa agggcaaaat caagaagtca ctggcgaaac cactttgacg aaagaagaca
    79681 aagatactgg ggataaagca caaggtaagg cagtctttga gggtgctgaa tacaccttgt
    79741 ttactgctaa ggatggaaaa gctgttaaat ggagtgaggc ttttaaacca gagttagcga
    79801 agggaacgaa aggttctgat gaaacggtca ctttgacttt agacgaaaag aaccaagttg
    79861 ccgttaaaca tttagctatc aacgagtatt attggcaaga aacaaaagcg cccgaaggat
    79921 atagccttga tgaaacaaag tatcctgtat ccatcaaaaa ggtggatgat agcgaaaaaa
    79981 atgccgtgat tacccgaaat gttacggcaa aagaacaagt gattcgcttt ggctttgatt
    80041 tctttaaatt tgctggatca gctgcgggca ctaccgaaac tggcttcaat gatctgacct
    80101 ttaaagtgtc gccattggaa gggaccaatg aaatcacagg tgccgaagat gaagcgacca
    80161 cagcttataa cgagcaatta gggtttgatg gttatggcaa gtttgaaaat cttccttatg
    80221 gggattattt acttgaagaa gtagaagccc cagaaggttt tcaaaagatt acgccattag
    80281 aaatccgttc tacttttaag gaaaacaaag aagactttgt gaagagtgag tatgtcttta
    80341 ccattaccga acaagaccaa aaacaaccaa ttaaaacggt gaccgttcct tacgagaagc
    80401 tgacgaacaa agcattttct gttagtttga accgtttaat gctctatgat ttgcctgagg
    80461 aagaagatag tttaacttct cttgcgactt ggaaagacgg aaacaaagaa ttaacttctc
    80521 tggattctac cgagctagtt gataaattga gttacaatct acatgaaatc aaagaagact
    80581 ggtatgtggt agcgcaagcc attgatgtgg atgcgacaaa agctgcccaa gaaaaagatg
    80641 aaaaagccaa accagtggtg attgcagaaa caagcgcaac gttggcgaac aaagagaaaa
    80701 caggaacttg gaaaattcag cataaattaa ccgccgaaca agttttaaat aaaactattg
    80761 tcttattcaa ctatgtgtat gaaaataagg aagcttttga agcaggcgat aagccagtag
    80821 cgaaggatgt tagcttgaat aaccaagccc aaactgtcag ttgtacagtg gaacaccatg
    80881 tttctatcca aacaaaggcc cacctagaaa acggttctca gacctttact catggtgatg
    80941 tggtggatat gtttgatgat gtgtcgatta ctcatgatgt actggatggc tcaaaagaag
    81001 ctttcgaaac aattctgtat gctttattac cggatggtac aaacaaagaa atttggaaat
    81061 ctggcaagat tgattatgaa gtaaatgata aagaattcac caaaactgtg cttgcgaaaa
    81121 aagtagatac cggaaaatat ccagaaggaa ccaagtttac ttttgcggaa atcaattacg
    81181 ataaagatgg gaatatcaat ggaaaacaca atgaagattt gaaagaaaaa tctcaaacct
    81241 taacaccaaa agaagttcca accatactaa gtacgccgaa acaaccggaa acaccaactg
    81301 ttccaagtgg ctctcaagaa tctagtccca cagtgaagac cttccctcaa accggggaga
    81361 aaaattccaa tgttttactg ttcattggtt ttactttgat ttttgcgacg gcgggctatt
    81421 atttctggaa tcgccggaac taaggtgatg agatgataca agtaaatcct aagaaccaac
    81481 gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt gtatccaaaa ctaacaatga
    81541 aaagaggaaa agaagatgga attgaaattt gtcgtgccag atatggctga aacctttgga
    81601 aagttaagtt atgccggtga aggagaaatt ataacagaag gatatggacg gaatactacg
    81661 gtgattggtc gcagttatca tttgtattcc agtaagcaac gggccgatga tattgaggtg
    81721 gtagtagctg cggaagccgg tgaaaaggat tttgatcaag accaaccgct aaaagccgtg
    81781 aatccccatt tggttgccaa aggctatgag attgagaatc gtgggtttac tgattacgtg
    81841 ttgtatgttg atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt
    81901 attgtcgtag attcaggttt aacatttggg aagttgcgtt tttccgcatt acgccgagaa
    81961 gtacggaagc aaaatgagga tggaacaatt ggtaatgagg taaaagaacg aacctataat
    82021 ttaaaatcct ctgcacaagg gcgaatgatt caagttagta tcccagctaa tgtaccctta
    82081 cgcgagtttg cttatgatgc agaggtagag ttggtcaatc caatcgtgga taccgttgcc
    82141 aattatgttt ttcgagaagg aactaccgtt aattggttca ttaaggcgga tgatttagtc
    82201 ttgaagcggc agccgaatca aggaaatcct acgaaccaaa atgaaagaaa gaaataggtg
    82261 aagattatgg aaattaatgt tgaacatatt ttagattgtc tcgatcaata tggaaaaggc
    82321 gagctgactg aggaacaact cgttaaagca ttaacctctg atgaaaaaat gtttctgatt
    82381 atacatcaag gtttacttgg agaaaatagc gacagtgaag aagactctga tgtactagac
    82441 tggttaggag aagaagagtc tttctttata gttgtcgaag taaatgaaaa cttatgtcgg
    82501 caggcagaat ccattttgga agaaattggt gtggagatgc cggatgctat tgaaggcttt
    82561 ttaaatcaat tggttgaaac gaaacaactc ccctcctcgt agcagtaaat gactagcata
    82621 actttttgaa attgcctaat gaaatcctat tgggcttttt cgattggagg tggaaaccat
    82681 caaaatgtac aaaggacatc gcatacgcgc aggagatcag cacttggttt atcactttgt
    82741 tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt ttcaagagtt
    82801 tagacaattt gatatttcta agctatcact ttctactatt gaaatcgttt ggtctataaa
    82861 agatttagta tgtctgcttg gaagtctcgc tttttcagga gctatgatat tgctttatat
    82921 tcactttttt cttgatcatt ggcgaagctt gtggcatcga caaaagctag ctcggatgat
    82981 tctagagaac cattggtatg aagtgaaaca aactcaaagt gaaggctttt tcaaagattt
    83041 gaatagtagc cgaaccagag agactatcag ttacttcccc aaaatctgtt atcgaatgaa
    83101 agatggatta ctgtctattc gcgttcaaat ttcacttggg aaataccaag atcagctttt
    83161 aaaattggaa aagaagttag aaagcggact gtattgtgag ttagtagaaa aggaactcaa
    83221 agactcctat gtggagtata ctttactgta tgatatgatc gccaatcgaa ttgggataga
    83281 cgaagtagtg gcagaaaatg ggactctgcg attgatgaaa aatcaagtat gggcttatga
    83341 ttctttaccc catatgttaa tcgctggtgg cacgggtgga gggaagacct atttcctcct
    83401 caccattatc gaagcactat tgaagtcaga tgcagaattg tttatcctcg atcccaaaaa
    83461 tgcagattta gctgatttag gtacggtgat gcctcatgtt tattcacaaa aggaagaaat
    83521 ttctgcttgt gtggaagatt tctatgaacg tatgatggct cgtagcaagg caatgaaaga
    83581 aatgtctaac tacaaaacag gagagaatta tgcctatctt ggacttccac ccaacttttt
    83641 aatctttgat gaatacgtgg cttatatgga gatgttaacg acgaaagaaa gtgcggtgat
    83701 tttgaataag ctcaaacaaa tcgtgatgtt gggtcgtcaa tctggtttct ttctgatttt
    83761 ggcttgccaa aggccggatg cgaaatattt aggagacgga atccgcgatc aattcaactt
    83821 tcgcgtggct ttgggtcgta tgagtgaact cggatattcc atgatgtttg gggaggtcga
    83881 aaaaaacttc tttatgaagc acatcaaagg tcgaggttat gtggatacag gtggcagtgt
    83941 gattagtgag ttttatacgc cacttgtacc aaaagggtat gatttcttag attcaattag
    84001 taaattagaa aagatgaatg attgagtgat cttcaatact gactttatac ttcaagtaac
    84061 gtataatact tttatatagt aggaagaaag gggataatta atatggaaag taatgattct
    84121 tatttagagt atgcaaaagc aaataaggat aagtttattt cttatattat caaagggaaa
    84181 tcccccgaag ttggacgaga cgcagttttt atggcaggga gtcctggggc tggaaaatca
    84241 gaggttgcca ttggattagc tgagaattat gataaccatg tgattattga tgcggattac
    84301 tttcgcaccc agtttcctga ttataatgga aaaaactcta gtctctttca aaaagcatct
    84361 tcttggttgg tagagcaggc atttagatat gtcttagatc atggctattc ttttattctg
    84421 gatgcaactt ttgcaatatt aagtgctgaa aaaaatatct cgcgggcgtt gaaaaaacat
    84481 tttcgagtga cgattttcta tgtttatcaa gaccctaaaa tcgcttggaa atttacacaa
    84541 gaacgtgaaa ttgcagaggg acgtcatgtg ccgaaaaagg catttattaa cgcttttttc
    84601 aaatcaagag agaacattgc aaaagtaaag agtaaacatc cagatgtatt gcttcatatc
    84661 ataataaaag attatacgaa taatatttcc gaagttcatt atgctacgga taatctagaa
    84721 ttatttttac cactagggta tagtaggaaa gatttggagg ttcaattaca tgactgagaa
    84781 agaacaagta acgaaaattg taaagaaata caataagagc attgcggatt tatcggaaaa
    84841 tgctactgca aaagagttca aaacggtgat taaatatgta gccgatcagg ccaatgaaaa
    84901 acaacggaaa ttagttggat taaataaaaa atagtaggac aaattaataa tgtatgagcc
    84961 aagagcaaaa tgttcttggc ttttttcttt gcaatgaatg aggtaatgta tgagattttt
    85021 agatttattc gccggtattg gtggctttcg tttaggtatg gaacaggcga gccatcattg
    85081 tatcggtttt tgtgaaattg ataaatttgc tagacgtagt tataaagcaa ttcatgatac
    85141 aagtaaggag gtggaaatgc atgacatcac aagtgtatca gatgagttta ttcaatccct
    85201 cggaccagtg gacatccttt gcggcggatt tccgtgccaa gctttttcaa ttgcgggaaa
    85261 gcggcaaaga ttttccgata ctcgaggtac tttattcttt gaaatcgctc ggttcaccac
    85321 tcttctccga cctaagcttt tattccttga gaacgtccga ggattgctca atcacgaagg
    85381 aggggctacg ttcgagacga tcctccgaac gctggatgga ttggggtatg acgtggagtg
    85441 gtaggtgctt aactcaaagg cctacattcc ccaaagccgg gagcggatat tccttatcgg
    85501 acattctcga gacgcatgta ccgaacaagt atttcctatc attggaccgt cttcaacatc
    85561 tgatcaaaac atcagaaatt tggtaaatat caatccttcc aatcgaggaa tgggtggaca
    85621 agtctatggt tcagatggcg tagcgccaac cttaacaggt gatgaaggaa tcaaaatcgc
    85681 actgccagca agggatggaa tctctgtagc aggactatta ccgggaaagt ttgaacaagg
    85741 taatcgagtt tatgaagtta ctggaaccgc ccccaccttg tcaacgaaac aaggtggtac
    85801 aaaaatcatg attcgaaaaa atgaaacaga atatcaagag gtgaagccag aagacagtgt
    85861 gaatctggct tttcctaatt ccacaagtcg ccgaggtcgt ctgggaaaac agagtgttca
    85921 taccttacta acaggagacc aacaagcgat cgtcacggat cagtatcaaa ttcggaagct
    85981 aacacctaga gagtgttggc gacttcaggg ctttcccgat tgggcttttg atcgagattc
    86041 tcaagtcaat tcagatagtc agttgtataa gcaagccgga aactctgtaa cagttccggt
    86101 catttttgat attgccagaa gattaaaaga gttggatgta catggtttat gatgagttga
    86161 ttggagaaat ttattgggtg attggaaaga ttcagtcaga cccagaatta gaagaacaat
    86221 tgcgacggtt aaaatttgat attcgcaaga atggtgtcaa agtcgctggc gatccatatt
    86281 taatgaatga ggagacggat gctcgaattg aaataaatca agtaattgca gaatttgagc
    86341 ggattgcgga ccaagcaaaa gaaccagata ttcgtcaata cttgttagaa atgaaagcag
    86401 agttggaagt aaatggtatt acagatgagt aacctaaacc aacacaactg actgttaggc
    86461 gctgaccgcg gaggattcgc gaagcagaaa tcctccgtgg tgctgtgcct gttcatggac
    86521 ggcgttagcc gaccatgatg tctcaccccc gtatctaaca ggggggtaca aaaacacaaa
    86581 aaataagtaa gcaaccatta agaatgcttg ttgtatcaaa gatagtaagt aatttgtata
    86641 ggtgttaact catttgaaaa agttaacacc ttgaatttgg aggtgagaac ttggcacaac
    86701 ggaatttaga ttatcggcta ttaaaagacc gacgcaacga atacggcgtt tcacaaaata
    86761 aattggctac ggcttgtgga cttagtcggc cgtacttaaa tcagatagaa aatggtggcg
    86821 taaccgcatc tataaaaacc atgaggaaaa tttttgacca actggaaagc tttaatcccg
    86881 atttgccttt gacgttactg tttgactatg taaggattcg ctttcccaca acggatgcac
    86941 gaaaaattat tcaagaaatt ctccatttga aatttgagta tatgctccat gaagattatg
    87001 ccttttactc ctatcaagaa cagtatgtca tgggtgatat tgtggtgatg ttgtctcatg
    87061 aagaagataa aggtgtgctt ttagaattaa aggggcgagg ttgccgacaa tttgaaactt
    87121 ttttactcgc tcaaaagcgt agctggtacg actttttcga ggattgtcta aaggccggtg
    87181 gcgtgatgaa acgcttggac ttggcaatca atgatcgagt ggggctattg gatatacctg
    87241 agctaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc tttaaaagtt
    87301 atcgttccgg ggaactttta aaggctgatg aaaaggacgg catgggcaat accttatata
    87361 tcggcagtct caagagtgag gtttatttct gtttgtatga aaaagactat gaacaatata
    87421 tcaagttagg gattccatta gaccaaactg agacgaaaaa tcgttttgag attcgactga
    87481 aaaatgaccg tgcttttcat gcgattcaag atttgttaaa aggtcgtagc attgagagca
    87541 ccacgttttc cattatcaat cgttacttgc gatttgcgga taaagtagaa ggtaaaaggc
    87601 gaaccaactg gcccttgaat gaacaatggg ggcgttttat tggacggaat cggaaagaga
    87661 ttcaattaac ttccgagcca aaaccgtata ccattgaacg aaccttgaat tggctaggcc
    87721 gacaagtcgc ccctacgtgg aaaatggcta aggagttgga tcgattgaat cggacaacct
    87781 atatccagga tatggtacag aatgcacgct tatcagaccg acataaaaag attttggagc
    87841 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtca tgggaattgt
    87901 aaaagatatt tttttggtat ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    87961 gaatgcaagt gcagcagcgg atcgagcaat ggaaaagaaa ggaaagtgac aagatgaatt
    88021 tcggacaaaa tttatgtgcg actcgttcct tagtgaaaag ctaaggcact aataaaagaa
    88081 cgaaaaagtt tggttattag gagaactgat aatctgacag gtggaatgat gaagtaacgc
    88141 tctgaaacgc ctgccctgag cctccgacaa gcattgtatg aggaaactca gaatgtttga
    88201 agctcggtaa agacggctga aagataccgt aatagttgat gaacatatat cttctatcga
    88261 aaactatcca gaggtgggta gcgtatccaa caggtcgggg gtctataaaa tatctatggt
    88321 tagaatgttc tataaggaac tgacgaaaca gcgaatgtac gggtctaaat ttgaactttg
    88381 tagaaatgca aaggtacgag aaatcgtagc tggtgtggca aagtaagaat ataggttatg
    88441 aaatgccata taacgttaca ggcggtatca agccagcagg cttatagcta gtgacctaag
    88501 gatatatatg tatagataag attatcggaa cgaggaaagg tattgggttc tcatcaagag
    88561 aataaactga cgaagaacaa taagcagttg aactaatgct gaaaagcaga gggcgaacct
    88621 aagaaattct tgtaatgaga atggaggaat gccctcaagt cgtgtatata ttagaaaagt
    88681 attatgttgt ttcaataagg attacgagta agataaaaaa gctcactcaa tttcaagtaa
    88741 aggagtgaag ccttatgcct aaaaataaaa atgaaacatt gtgtgtagaa gatttaagac
    88801 acgctgaata ttacgaaatg caaaacacct ttgatgattt atatgccaaa agtaagaatg
    88861 gagatatttt cactcatctt atggatatta tcctatcgag agaaaatata ttactggcat
    88921 atcgaaatat caaagcaaat gcaggcagta aaacagcagg aacagacggt acaataatta
    88981 aagatattgg caaattgcca gcagaaacag ttgtcaaaaa agtaagatac attgttgcag
    89041 gcagtccaca tgggtatcga cctaagccag tgaggcgaaa agaaatacca aaaccaaatg
    89101 gtaaaaccag acctctaggt attccttgta tgtgggacag gcttatacaa caatgtataa
    89161 aacaagtatt agaaccaatc tgcgaagcta aatttagtga aaacagctat ggttttagac
    89221 ctaataggtc agttgaaaat gcaataaaag caacctataa ccgcctacaa atcagccagc
    89281 tacattatgt gattgaattt gatattaagg gtttctttga caacgtgaac cattccaagc
    89341 tgataaaaca gatatgggct atgggaatac gggacaagca cttgatattc attttaaaaa
    89401 ggatattaaa agctccaatc aaaatgacta acggtacaat aacatatcca gagaaaggaa
    89461 ctccgcaagg gggaataata tccccactac tagcaaacat tgttcttaat gagttagacc
    89521 attgggtaga aagccaatgg caggaaaatc ctgttacaaa aaactatgtg gttcatatca
    89581 ataaaagcgg tagcccatgt aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa
    89641 aggaaatgta tatggttcgt tatgcagatg attttagagt tttctgccgc tacaaggaaa
    89701 gtgctgaaaa agccaaaata gcaatcactc aatggattga acaacgatta aaattggaag
    89761 tatcacaaga aaaaacaaga attgtaaatg ttagaaaaag atactccgac tttcttggtt
    89821 tcaagattaa aatgataccg agaagaaaga agttagttgt taaatcacat ataagtgata
    89881 aacaattcaa aaaccaaaaa gacaagctgg tatcacaggc aaagaaaatt gcttctccct
    89941 caaaagggaa aacggaatta gaagaaacaa gactttataa ttcaatggta ttaggtatgc
    90001 aaaattatta tcagattgca acgaatgtaa atctggattg tatgaaactg aatagagctg
    90061 taatggctat cttcactaac agattaggta caaacaaaag gggcagattg agaaaaacag
    90121 gcagacaact tacgacaaag gaggcaacaa gatatggtaa atctaaaatg cttcggtatg
    90181 ttgctggcga gggcgaacct attttccctg ttggatatat ccaacacaga aaaccaatgg
    90241 caaaggtgta tttagcgaat tgctacactc tagagggaag aaaatttatc cacaccaatc
    90301 taagtgttga taaattactg atgtaccagt taatgaaatt atctttggaa aatcgcacaa
    90361 tagaatacgc agacaatcga atttcgctat tttctgctca acatggaaaa tgtgctataa
    90421 ctctggaaga atttcaacaa gctaatgaaa tacattgtca tcatattgta ccaactggag
    90481 ctggtggaaa tgacgattat aaaaatctta ttttggtaaa agaggcggta caccgtctaa
    90541 tacacgctaa aacagaagaa acaatacaaa aatatatagc tctattgaag ctaaataaag
    90601 tccagctcat aagactaaat aaatatagag tattagctgg caatcaagaa ttaaacttga
    90661 tataggtcta ctacataaat acttttcaat ataaacacga tggaacgcgg agtgcggtga
    90721 aagtcgcacg ctccgtgtga atggggggaa aagtttgaga tatttataat atcaggaact
    90781 tacctatcca tatatcaatg gtttttaacc aatgcccaat ccttagtctt actggcaatt
    90841 gtggtcattg gtttgttttt aggatttaag cgggagtttt ccaaactgat tggctttttg
    90901 gtgattgctt tgattgcggt cggattggtc tttaatgctt ctggggtgaa ggatgtctta
    90961 ctcagtttgt ttaaccgaat tgtcggtgcg taagggggaa gcacgatgga acagatgcgt
    91021 gtttatattg cgaacctcgg gaaatataac gaaggtgaat tggtgggggc gtggtttacc
    91081 ccacctgtcg attttgatga agtgaaagaa cgaattgggt taaatgacga atacgaggaa
    91141 tatgcaattc atgattatga acttcccttt gagattgatg agtatacgcc aattgaggaa
    91201 attaatcggt tgtgtggctt agcggaggaa cttgaaggaa cgccagttgg tgaagttgct
    91261 tctgagattc aacatgcttt cttcaattct tttgaggaga tggtagagca tgtggatgat
    91321 attatctatt atccagattg cgatgatatg agtgatgtag catatttctt gattgtagaa
    91381 gctggtgatt ttggagaagt accagaacac ttgaagacgt atattgattt tgatgcgtgt
    91441 ggtagagact tggagattgg aggcaattat ttggtaacga gtcgaggtgt ttttgagtat
    91501 ccatattaac aaaattattg aagggtagtt gatttctaaa ctatccttct ttttaaagga
    91561 atgatttgat gaagaaaata aaaagctaca ccagtatttg ggcagttgaa aaggtcattt
    91621 atgcaattaa tgactttcaa ctgccttttc ctgtgacttt taaccagatg gcttggtttg
    91681 ttttatcctt gctgtttgtc atcgtatttg cccatgtgcc tccactatct atgatagagg
    91741 gggccttttt aaaatactta ggtataccgg tggcagttac ttggttcatg tcgcaaaaaa
    91801 cgtttgacgg taagaaacca ttgggatttt tacgatcttt tattagctat cacttacgat
    91861 ttaaggtcac tttcgcagga aagaaagtca aagaacagaa gaaacgatgg gacgaaccca
    91921 ttactctggt aaggagtgtg aactatgtac ccgattaagt atattgaaaa taatcttgtc
    91981 tttaatcaag agggagaatg ttttgcctac tatgagttaa taccttacaa ttactccttt
    92041 ttgtcacctg aacagaaata tcaagtccac gataactttc gtcaattgat tgcgcaaaat
    92101 cgtgaaggca agattcatgc cttacaaatt gccacagaaa gtagtattcg tgccacgcaa
    92161 gaacgatcaa aaaaggaaat taccgggcga ttaacagaag tagcgaaaca acgaattgat
    92221 ttacagacag aggcattagt atccatgatt ggcgatagtc agattgatta tcgctttttt
    92281 attggcttca aactgattgc gacagatgaa gaggtcaatc taaagaatct caagaaatct
    92341 ttcttctccg gctttcaaga atttgtctat ggagtgaatc atcatttgat gggcgatttt
    92401 gtttccttat ccaatgaaga aattcgccga tataccaagc ttgaaaaact aatggaaagt
    92461 aaattggctc gaagatttaa agtcagacga gtcacaccga gtgatctgat gtatttgatt
    92521 gaacatatct atggcgaaaa gggtacacca tttgaagaat atgaatttca gctaccaaag
    92581 aaaaagctga agtcggaaac tttggtgaaa cgttacgact tattacgtcc cagtcgttgt
    92641 ttgattgaag aaaagccccg gtacttatgc atggaacatg agaatcatga atcatatgta
    92701 gcgtacttaa caattaatac aattgtgggg gaaatggagt ttccctcctc agaacttttc
    92761 tattaccagc aacaacaatt tacattccca attgacacta gtatgaatgt ggagattgtg
    92821 acgaacaaaa aggcacttgc taccgttcgc aataagaaaa aggaattaaa agacttggac
    92881 aaccatgcct atcaatctga caatgaaaca aattccaatg tcttagatgc attggattcc
    92941 gtagatgaat tggaaactac cttggatcag tccaaagaat ccatgtacaa actgagctat
    93001 gtggttcgag taagtgccga atcagtagat gaactaaagc gccggtgtga tgaagtgcta
    93061 gatttttatg atgataccaa tgtaaaacta gtccggcctt ttggcgatat gatggggcta
    93121 catgaagaat tcttaccttc tagtaagcga tacatgaatg attatatcca gtatgttacg
    93181 tctgattttc tcgccggact tggttttggc gccactcaga tgttagggga gttggaaggc
    93241 atttactttg gttataacgt agatacgggg cgcaatgttt acctgaaacc ggcattggcc
    93301 tcacaagggg tcaaggattc agttaccaat gcgctagcag cggcattcct cggttcactc
    93361 ggtggcggaa agtcctttag taacaatctc ttagtttatt acgccgtcct ttttggtgga
    93421 caagctgtga ttgtcgatcc aaaaggggaa cggggtggct ggaaagaaac cttgccagaa
    93481 attgcggatg aaatcaatat tctcaactta actagtaagc caaaaaatca aggattactt
    93541 gatccttatg tgattatgga gaaaaagaag gattctgaaa gtctagcaat tgatattctg
    93601 acctttttaa caggaatttc tagtcgagat ggggagaagt ttccagtgtt gcgtaaggcc
    93661 attcggacgg ttacccaaag caatcaacgg ggattattac aagtaattaa tgagcttcga
    93721 aaagaaggct caccagttgc ggaaaatatc gcggatcaca tcgagtcctt taccgattat
    93781 gattttgccc atttgctttt ctcagatggt tcgattaccc agtcgattag tttagatagg
    93841 cagttaaata tcattcaagt agcggatttg gttttaccag atgcagaaac cacttttgaa
    93901 gaatatacca ccatggaatt gcttagtgtt tccatgctaa tcgtcatttc cacttttgcc
    93961 ttggacttta tccatagtaa tcgagaaatc ttcaaaattg tagacttaga tgaggcgtgg
    94021 agttttcttc aagtagcgca agggaaaaca ttatcgaata agctcgttcg tgctgggcgg
    94081 gctatgaatg ccggggttta ctttgtcacg caaaatagtg atgatttact ggatgaacgc
    94141 ttgaagaata atatcgggtt gaagtttgcg tttcgcagta ccgatattca tgaaatcaag
    94201 aaaaccttgg aattctttgg cttggatcga gaagatgaaa gcaatcaaaa acgcctacga
    94261 gatttggaaa atggccaatg cttgatgcag gatttgtatg gtcgggtagg cgtggttcag
    94321 gtacatccag tctttgaaga actcttccat gcctttgata cccgtccacc tgttcaagag
    94381 agaatggagt gaggggatga aaaagaaact ttttcggatt gtgggaatcg tttttattag
    94441 catactcact ctgatagttc tattgagttt agttggtacg gtagccgagg cgacagggct
    94501 agttgatgat acagttgaag caggaaatct atattccaaa tattcactga acaactatca
    94561 actagatttc tttgtggata gctcttggga ttggttgcct tggaattggg gtgatggctt
    94621 agggaaaagc gtgatgtatg gtctttatgc cattactaat ttcatttgga cggtgagttt
    94681 gtatttgtcg aacgccacgg gttatgtggt tcaagaggct tacaaattag atttcatttc
    94741 ggatacggct gaaagtattg gaaagaatat ccaaacattg gctggtatta ctgaaaatgg
    94801 actccagacc tccggatttt attttgggtt tctattattg atgattttag ctctgggggt
    94861 gtatgtggcg tataccggat tattaaaacg agaaaccacg aaagcagttc gagccgtgat
    94921 taattttgtg atgatttttc tactatcggg ttcgttcatt gcttatgcgc ctacctatat
    94981 cacaaaaatc aatgacttta gctcagatgt tagtgaagcg gcccttaccc ttggaaccga
    95041 gatcgttgta cccaattcag aaagccaagg aaaagatagt gtggatttga ttcgggatag
    95101 tttgttttcg attcaagtcc aacaaccttg gttactactg caatttgatg attccaacat
    95161 agaagaaatt ggtgaagacc gggtcaacaa gattctatct gtgagtccag atgaaaacaa
    95221 aggcaaagat cgggaagaag cggtcaaggc agagattgaa gacaatgaca atgcgaatct
    95281 cagtattacc aagaccatga accgtttagg gaccgtggtc tttttggtat tgttcaatat
    95341 tggcatttcc ttctttgttt ttcttctaac agggattatg ttgttctcgc aaattctctt
    95401 cattatcttt gcgatgtttc tgccaattag tttcttattg tccatgttgc cgacttatga
    95461 aagtttagga aagaaggcga ttattcgttt attcaatacg attatgatgc gagcgggtgt
    95521 tactttggtg attacgactg catttagcat ctccacgatg tttttcaata tttcggcaac
    95581 ctatccattt ttcatggttg cttttctgca aattgtgacc tttgccggta tctatttcaa
    95641 attgggcgac attatgagta tgttcaacct ccagagtaat gatagccagt caatgggaag
    95701 acgcgtcatg cgaaaaccgc aaatgttaat gaatcgaaaa ttgcggcaac tcaatcgaaa
    95761 tgtgggacga gctttggctt ttggtggtgg tgcagcagtc ggaaataagt tggcaaagga
    95821 acaatcaaaa tccaaattta agccatccgg ttcttctcta cgaaagaatt cacgattacc
    95881 gaacgatcac gaaatactat ctgacttaac taaggagaat ccagtttcaa ataataagaa
    95941 acaatcccga atgaatttgg ctgggagaaa aatcggcaaa gttttggata cccaagcttt
    96001 ggtgaaagat aagactaaac aagtaaagga tcaggtaagg aatacgccaa cgaatttgaa
    96061 atacaatctc cataaaggaa tcgaaaagtc gaaaaaagca ccagaagaat tcaagcgcgg
    96121 actgttccaa gaaaaagcgg atcgagtgga tttgcgagaa aaacaacgac aacgaagaaa
    96181 tgcgaaaatg gacgaaaaac gcaagatact gagtgaagca acaaatcgtc agggaaaaag
    96241 aagggcgaat attccaatag ataaagcagc tcaacctcag aaagatatga tgctgaaaaa
    96301 agaagcacca aagcggattg tgagagcaaa tctgaatccc gaaatcaaac gaaaactaac
    96361 tagtcaagaa atcatttcaa aagggaaaag ccaatcagca agtaagaaaa attctttcca
    96421 acaagtgaaa cagaggtcca ctctgccaaa gcagaccaat caaaaagtac aaaaaagaat
    96481 gaaccatcgt ccaaaatcaa ggtcaggtga gaaaaaatga acttgaagaa agtgggtgtt
    96541 atttcattaa cttttctgac catctctttt atgggtattt tgatgtgtgt gggtttgttg
    96601 tttggtgaag acagtgaggg aaatggtagt tctggcactt ctcctggcgg aactagtgtc
    96661 tcagaagaag tattgaaaca ccgtgcaatg gtggaaaagt atgccaaaga atcggatata
    96721 acagagtata ttccaattct tttggcgatt attcaagtgg aatctggtgg cacaatggaa
    96781 gacgttatgc aaagttctga gtcagccgga ttaccaccta attcattaag taccgaggca
    96841 tccatcaaac aaggctgtct ctattttgcg tctttggtta agcgttccaa agaactaggg
    96901 tgtgatcaag atagtattat tcaggcctat aactatggtg gcgggtatct agatcatgtc
    96961 gggaaaaacg ggaagaagca tagttttgct ttagctgaat ctttttcaaa ggaaaaatca
    97021 ggtggtcaaa aagtcgatta ccccaaccca atcgcgatta aggaaaatgg tgggtggcgg
    97081 tataattatg gcaatatgtt ttactgttta ttagtgaagc aatacctaac tacaactcag
    97141 tttgatgaca aaatagtcca agaaattttt gatgaggcat ttaagtatga aggaacggcc
    97201 tatgtgtttg gtggctcaag tccagaaacg gggtttgatt gtagtggctt gacccaatgg
    97261 tgttatgcca aagttgacct gaaacttcct cgggtggctc aggatcaata tgatgccatg
    97321 actcatattg atctaaaaga tgccaaacca ggtgatttag ttttctttca ttcaacctat
    97381 gatgccggta cttatgtgac ccatgtgggc atttacgcag gggagaatcg catgtttcat
    97441 gcaggagatc cagtggggtg gacgaatcta acggaaaact attggcggca gcatcttatc
    97501 ggagctggcc gatacaaata agagaaaaga ggaaaaagaa tgaagataaa aattgaacgg
    97561 aatcaaaagg aaaagagccc aaaaaagaaa aaagaacgag ttgtatccgt gggtaagcat
    97621 cgaaaaatgg tgcttgccct ttggctttta ttgtcttgta gtcttgcttt tggcgtttat
    97681 aaaaatttca ccgccattga tcaacataca actcatgaaa aagtggttat aaaagagaag
    97741 gtagtgaata cttctggcgt tgaaagtttt acagaggatt ttgcgaaaga atatttttca
    97801 tggaaaaata acaaagaagc aattgaaaag agaatgagta atctcgaaca gtaccttaca
    97861 gaaaacggtc tcgctttaat ccaagatatg gttcgagcag atattcccac gagttccgaa
    97921 gttcaatctg taaaaattct agatgttgaa aaacactcgg aagaatttgt cgtttcattt
    97981 ttagtggagc aacatattac agagggaaaa aagacgcaat caatttcttc tgcatatcga
    98041 gtgacaatct ttgaagatga aaacaggaat catttagtaa ctagtttgcc aacaatgatt
    98101 ggaaagccag acaaggcaaa atatgaagca aaacaagtag aaaacgattc agaaattgat
    98161 gccaaaacaa cggaagaaat caccgatttt ttagaaacat tttttaaact ttatccatca
    98221 gcttctgaaa aagaattgga gtattatgtt gaaaatggtg cgatgcgacc aatcaatatt
    98281 gacttaaagt tttcggagtt agagaatcca gtttatctac aaaacggaaa aaattttcta
    98341 gtgagtacaa agataaaata ccttgataat gtatcaaaaa ccactagcta ctttcagtac
    98401 aaattagtat tacaaaaaaa tggaaactgg aaaatcgttg atgttaaata ataatgactc
    98461 aatctaattt tattagaagt tataagtagt gtaacttatt tcaggcagaa gtcgcccatc
    98521 ctctataggt gggtgatgaa tgccattcgg taactcgaaa accgacaatt aagacatagt
    98581 gcctttttat tttaatccat gtcataatca gtctatatag gaaatggttg atataccatg
    98641 gaattagata caaataatca ttcagttttt cttcttggtt acccccttat tttagtagtg
    98701 aaacattgta aacatgtcat tgatgatgta atgtcagctt atgcaaaaac tgcctttgaa
    98761 agaatttcag aatcacatca cattacctta gatgaatgaa atcgtgataa agaatacttt
    98821 catattatgc tcaaagcaca acctaaaacc gaattaacaa aattcagcaa tgcctaagct
    98881 cacgtctgat aaaatgagac tttctaaggg tcaaaaattt cctttgaata cgattattgc
    98941 ttagataaaa gtaaaagcct tagaactacg gggatagctt ggttaataag agatatcgtt
    99001 gttagtaaag acataggctt gcaagtatgc tctattccca agaatctccc ccttcaagca
    99061 ccaaggatcg ttaaattcag ctaagtggca agtaattcac tagacgataa ttatctccat
    99121 atttcaatct cagcttttcc atttttcata gcaaattccg ttatttttat agcatctaaa
    99181 agattcagct tttccactga tctttcacca cttttcagct tttttattgt gcttaaattg
    99241 ataccagtac cttttgatat tgaattggaa gtaaaatcat taagggtttt ttttattaaa
    99301 ttcatatccg caattccagc gttcataaga atgaagattc tcctctcata gactactttg
    99361 aaaatggtac tacagtggct gtttatatat ttcagtaggt tactgtagtg gtcatcttat
    99421 tataattaga aaaggaacct aaatcaaaaa tgctaaagaa aattttcata gtattaagcc
    99481 tctcatgact aaagccacgg aattcttgat aaaagagtat acttgcaagc ctaaacagcg
    99541 gtatctctta ttaaccaagc tatccccgtt gttcctaagg ttcttgatgt tttctaaggc
    99601 aatgcttgta ttcatagccc ttggtcaaaa tattgatact atcgttgacg tttcgattat
    99661 gatgtatacg gcaaatagga caagtccatt ctcgaattgt gagtgacttc ttaccatctg
    99721 tgtgtctaca ttctgaacca atttgactag acggaaatca tttatctatc ttaatgattt
    99781 tttgacagaa gtatcgaaaa tctttacata atctgggtga tgtattctta tgcagatgag
    99841 agcgttggct ccgtattcat aaccagtagc ctctaaaacg tagtccatcg gtgggatacc
    99901 tagcttgcgg ttgccagcgg ctttcgcagt cttcttgtga tcaaatcaag catctcattg
    99961 agcactagta ttttgaagat aatacttgag tttttcggct tttagaagtt cacttcatct
   100021 tccatcatcc atggaaatcc cactcaccca tacgcaggcc tccaaaaatt cttcaacagt
   100081 ggttttttca gacatttttc tgaatacctc cttgtttttt gatacgccag atagtcagga
   100141 aaaaggcaac aaaacacaaa acaaagctgc ctaaaaaggt aatcttcatt ccatataaaa
   100201 agacatccgg ccgatttgcg atatagttag ttacttgctg gcccatctta gcagacatcg
   100261 cttgatataa gaccgtcgta gaaagcgaaa tcccgctgac catccccaaa tttcgggcaa
   100321 aggaattcat cgagcctgcc agtcccaagt tttcgatagt gaccgagctc ataaccatcg
   100381 tattattggg ggactggaac aaggcattgc cggcacccat gatggcagta gcgacaatgt
   100441 atagccacaa aggagagtcc actgacaaaa acatatacat gacctgggtt accgacaata
   100501 agcccaagcc tactaatacc aaaatctccg ggccaacccg gtcggtcagg tagccgctta
   100561 aaggcgcccc gacgaccatc atcaaaggaa agaccatcat caacagccca gcatagctag
   100621 gactcaaccc gcgagcattt tgcagataaa aaggaatcac tacattgaca aagaagttgg
   100681 aagaaaaaat caacaaggct gtaatcaagc tcatggtaaa aatcttgttc ttaaaaatac
   100741 cgaaagaaat caatggagag gctgctttct tttcaacttt taaaaagctg acaaaggcaa
   100801 tcactgaaat tacgaacagt acaaggggga ctggatggaa aaagccactt tcttgcccca
   100861 aaaagattgc tccaaaaaag ctgataatcg tagccgcaat ggcagcaaag ccccctccat
   100921 cgattgtatg gccgctcatg gtgatatctt ttggtaagaa tttttcagca atcaacatcg
   100981 ttacgattcc caccggtaca ttgatccaaa aaatgtacgc ccaagaaaat tgggacaaaa
   101041 tcaagccgcc aagacctggt ccagcaatcg agcctaatga aacaaacgca cctattgctc
   101101 ccaatgcccg accacgttca tgtatgggaa agacttccgt gatgattccg gcatttgtag
   101161 ccatagtcat actagcacca atggcttgta ccacccgtgc gaacaataaa atacctagcg
   101221 aatgattgaa cccacacaac agcgatccca agacaaagat cagcgtacca atccgaaata
   101281 ctttgatttt cccacggcta tcgccaattt taccaaacag caacaaacaa gaacagacaa
   101341 tcatcagata aacggacact acccattccg cttgattcat tggtatgccc atatcatctg
   101401 agatggtagg tagtgcaata ttgacaatac tgccatccaa tgtggacatg aaggtaaaca
   101461 tagatatagc aatcaaaatc caccagcgat ttttttggac ttttgcatct tcttgataac
   101521 tatacataat aggatttcct ttctaatgat cgtgtagcta agaacaatat aacctatgtc
   101581 tactgcccaa caacttctcg cgactctagc cataggaagc ttgagtaatt tcgatttgat
   101641 atcctcatgt aaccatgtaa gttggagatg ctttatttga acttactcta tagtaactag
   101701 tgataattcg ctttactttc ttctagcaat ttctaaaagt cgacttcatt gttcttcaga
   101761 ttaatagtgt gagctatctc gtgagacgta agcaaatttc cattctactt tttcccaatt
   101821 ttttcatatt tatggtgttg cacctatttg ttttccaatt tgcttttcaa atacattgct
   101881 atgatattca attccatagg attatactaa atatcaatag tatgaataat atattataga
   101941 tgtttatata tcactgttaa ttattatcca caataattaa tgattacctc gatatttgtc
   102001 aatcaaaggt tgaaacaatt tattttcagc atcttttcta gcttgagctg cactcttaat
   102061 agaatcatac gatcctaaat agtacctctt tttttttaaa gttatatacg caatctattt
   102121 ctctttagaa cgataaactc ccttaacacc acttgtatta tttttcggta attttgacgt
   102181 tagacactct attactgtcc cttcaacaca tgtccttgtt tcttgccttt ttaaacatcc
   102241 acaactttga gtatgtccaa aaaataagtt actataactt atctcacaca tactgccaca
   102301 ctcacaatga caaacccatt ttgttttcca tgaacagtta ttgtccaaag gatactttgc
   102361 agtaagtaat ccgaattttt tattttcaat gtgatgcttt tttgatttca aacatccaca
   102421 gctttttaca tcacctttta gaagtcgctt tgccgatact aacttttttc tcccacacag
   102481 acattgacat tcccaaacaa cagttccttc acttcgcagt ttagttggct tgattgcaac
   102541 taacttacca aaagttttat ttgtcaaatt tttatattta tgcctatttt tataatttcc
   102601 acatccacaa cttttggttc tacctgatag taagtttgat cgatatacaa tcgtttcatt
   102661 cccacagtca cataggcaac gatatttttt atatccatta tgagtaatct cctcaacaac
   102721 agtaaggtga ccatatcttt ccttcactgc acctaccctc ccaagaaaga agtaccatat
   102781 aatttcaatt aacttttaat aaatcagaaa aaaatatttc caatttttct tagtctgatt
   102841 ttatgactaa tttacaatac aagactgaac gatacgtaca cgaaatcctc aagatagata
   102901 aattgttttc atctagtgaa acctgctcaa aatatgagca taaagacggc aataatacac
   102961 tcgatattcg ataatggact tgttttattt gtcaaaccca tcgtaatcga gatatcaatt
   103021 ccagtctcaa tatcctgatc aaaggtctaa gaatccaacc attttcttgt tactttgtca
   103081 gattgattac cttttttagt ctccgaataa taaggttttc ggatcaacta acatgctctg
   103141 agaaagacta ctatctttgc aaatttccaa atgaaggtgt gggccagtac tattcccagt
   103201 acttcctaca tagccaatta tttgtccttg caaaacatta tctccagttt ttactaggta
   103261 ttgttgctgg tgagcatata acgcggttga tccatcttcg tgttgtatca ccacgtaatt
   103321 tccccaagag tagtgaaact ctgctttaac aaccttacca gctttaactg catgaatcgg
   103381 ctcaccctgt gtagctgcca tatcaagtcc tcggtgaaat tcatttccac gatttccaaa
   103441 caaactggtt attgaataat tatgaatagg cacttggtat ccagtcttac tcatttgtgg
   103501 acctttaact tctttatcat agttggttaa atcgtaggtt ttaattaatt catttagctt
   103561 caaatagtat tgtgtatctg ttgcataccg tcctgtaagg aatttagttg cttctcgata
   103621 tgttttacta ttagctttta atgctcctgc ataaaaatct ttatttccac taattccctt
   103681 agttaataat ttggcataat cttcaaagct ttcctcatag ttttcataca ctctaaattt
   103741 tgattgggtc gtataaagcg ttccattacc caaatcttcg tttgttacca taatgacaaa
   103801 gtttccatta taagttcctt ttatcccaaa taaattgtaa ttcggtgcct gagctaactg
   103861 gctttgacca ctggcagatt ctaagatcgc ttgtgcaatc ataacagaag catataaatc
   103921 atatttctga ccaatttttc tcgcagactc accaattttc ataataaaat tttctacaga
   103981 ttcatttttg tcaaaatgaa tttcttcatt accagatagt attggttgtg attggaaaga
   104041 ttctgttgat tctatttgct gtggtgtcga aaaagttttt gtttgtcctt ccgtttttgg
   104101 cgcttccttt tttgaagatt ctatatggtt tggttttgtc actttcgaag aagttatttc
   104161 acttgtacta cttgaagttg ttgaagattc ggtacttgaa atcgtactac tttcacttga
//
LOCUS       DAZKMK010000003        77656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000003 DAZKMK010000000
VERSION     DAZKMK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.003"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..1644)
                     /locus_tag="ACG8V1_000200"
                     /pseudo
     CDS             complement(9..1644)
                     /locus_tag="ACG8V1_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1967..2446)
                     /gene="rlmH"
                     /locus_tag="ACG8V1_000201"
     CDS             complement(1967..2446)
                     /gene="rlmH"
                     /locus_tag="ACG8V1_000201"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0328329.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2874..4163
                     /locus_tag="ACG8V1_000202"
     CDS             2874..4163
                     /locus_tag="ACG8V1_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0328330.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4202..4807)
                     /gene="ytpR"
                     /locus_tag="ACG8V1_000203"
     CDS             complement(4202..4807)
                     /gene="ytpR"
                     /locus_tag="ACG8V1_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0328331.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5012..5476)
                     /locus_tag="ACG8V1_000204"
     CDS             complement(5012..5476)
                     /locus_tag="ACG8V1_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0328332.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5498..5818)
                     /locus_tag="ACG8V1_000205"
     CDS             complement(5498..5818)
                     /locus_tag="ACG8V1_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0328333.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5962..7041)
                     /gene="pepA"
                     /locus_tag="ACG8V1_000206"
     CDS             complement(5962..7041)
                     /gene="pepA"
                     /locus_tag="ACG8V1_000206"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0328334.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7241..7591
                     /locus_tag="ACG8V1_000207"
     CDS             7241..7591
                     /locus_tag="ACG8V1_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0328335.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7814..8761
                     /locus_tag="ACG8V1_000208"
     CDS             7814..8761
                     /locus_tag="ACG8V1_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0328336.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8818..10236)
                     /gene="pepV"
                     /locus_tag="ACG8V1_000209"
     CDS             complement(8818..10236)
                     /gene="pepV"
                     /locus_tag="ACG8V1_000209"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0328337.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10385..11230
                     /locus_tag="ACG8V1_000210"
     CDS             10385..11230
                     /locus_tag="ACG8V1_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0328338.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11234..11674
                     /locus_tag="ACG8V1_000211"
     CDS             11234..11674
                     /locus_tag="ACG8V1_000211"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0328339.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11724..11918
                     /locus_tag="ACG8V1_000212"
     CDS             11724..11918
                     /locus_tag="ACG8V1_000212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328340.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12054..12740
                     /locus_tag="ACG8V1_000213"
     CDS             12054..12740
                     /locus_tag="ACG8V1_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0328341.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13148..13837)
                     /locus_tag="ACG8V1_000214"
     CDS             complement(13148..13837)
                     /locus_tag="ACG8V1_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328342.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13812..15941)
                     /locus_tag="ACG8V1_000215"
     CDS             complement(13812..15941)
                     /locus_tag="ACG8V1_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0328343.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15989..17326)
                     /locus_tag="ACG8V1_000216"
     CDS             complement(15989..17326)
                     /locus_tag="ACG8V1_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0328344.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17578..17937)
                     /gene="rplT"
                     /locus_tag="ACG8V1_000217"
     CDS             complement(17578..17937)
                     /gene="rplT"
                     /locus_tag="ACG8V1_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0328345.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17990..18190)
                     /gene="rpmI"
                     /locus_tag="ACG8V1_000218"
     CDS             complement(17990..18190)
                     /gene="rpmI"
                     /locus_tag="ACG8V1_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0328346.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18262..18786)
                     /gene="infC"
                     /locus_tag="ACG8V1_000219"
     CDS             complement(18262..18786)
                     /gene="infC"
                     /locus_tag="ACG8V1_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0328347.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18830..18955)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19106..19738
                     /locus_tag="ACG8V1_000220"
     CDS             19106..19738
                     /locus_tag="ACG8V1_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0328348.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19740..20645)
                     /locus_tag="ACG8V1_000221"
     CDS             complement(19740..20645)
                     /locus_tag="ACG8V1_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0328349.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20730..21389)
                     /locus_tag="ACG8V1_000222"
     CDS             complement(20730..21389)
                     /locus_tag="ACG8V1_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0328350.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21705..22649
                     /gene="mvk"
                     /locus_tag="ACG8V1_000223"
     CDS             21705..22649
                     /gene="mvk"
                     /locus_tag="ACG8V1_000223"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0328351.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22642..23619
                     /gene="mvaD"
                     /locus_tag="ACG8V1_000224"
     CDS             22642..23619
                     /gene="mvaD"
                     /locus_tag="ACG8V1_000224"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0328352.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23632..24717
                     /locus_tag="ACG8V1_000225"
     CDS             23632..24717
                     /locus_tag="ACG8V1_000225"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0328353.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24714..25778
                     /gene="fni"
                     /locus_tag="ACG8V1_000226"
     CDS             24714..25778
                     /gene="fni"
                     /locus_tag="ACG8V1_000226"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0328354.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26027..26830)
                     /locus_tag="ACG8V1_000227"
     CDS             complement(26027..26830)
                     /locus_tag="ACG8V1_000227"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0328355.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26850..27692)
                     /gene="kduI"
                     /locus_tag="ACG8V1_000228"
     CDS             complement(26850..27692)
                     /gene="kduI"
                     /locus_tag="ACG8V1_000228"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0328356.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27708..28715)
                     /locus_tag="ACG8V1_000229"
     CDS             complement(27708..28715)
                     /locus_tag="ACG8V1_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0328357.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28722..29366)
                     /locus_tag="ACG8V1_000230"
     CDS             complement(28722..29366)
                     /locus_tag="ACG8V1_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0328358.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29466..30224)
                     /locus_tag="ACG8V1_000231"
     CDS             complement(29466..30224)
                     /locus_tag="ACG8V1_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0328359.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30351..31502)
                     /gene="fucO"
                     /locus_tag="ACG8V1_000232"
     CDS             complement(30351..31502)
                     /gene="fucO"
                     /locus_tag="ACG8V1_000232"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0328360.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31584..31895)
                     /gene="rhaM"
                     /locus_tag="ACG8V1_000233"
     CDS             complement(31584..31895)
                     /gene="rhaM"
                     /locus_tag="ACG8V1_000233"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0328361.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31897..32742)
                     /gene="rhaD"
                     /locus_tag="ACG8V1_000234"
     CDS             complement(31897..32742)
                     /gene="rhaD"
                     /locus_tag="ACG8V1_000234"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0328362.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32756..34012)
                     /gene="rhaA"
                     /locus_tag="ACG8V1_000235"
     CDS             complement(32756..34012)
                     /gene="rhaA"
                     /locus_tag="ACG8V1_000235"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0328363.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34009..35469)
                     /gene="rhaB"
                     /locus_tag="ACG8V1_000236"
     CDS             complement(34009..35469)
                     /gene="rhaB"
                     /locus_tag="ACG8V1_000236"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0328364.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35622..36491
                     /locus_tag="ACG8V1_000237"
     CDS             35622..36491
                     /locus_tag="ACG8V1_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0328365.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36617..37516)
                     /locus_tag="ACG8V1_000238"
     CDS             complement(36617..37516)
                     /locus_tag="ACG8V1_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0328366.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37681..38505)
                     /locus_tag="ACG8V1_000239"
     CDS             complement(37681..38505)
                     /locus_tag="ACG8V1_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0328367.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38516..39811)
                     /locus_tag="ACG8V1_000240"
     CDS             complement(38516..39811)
                     /locus_tag="ACG8V1_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0328368.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39827..40306)
                     /locus_tag="ACG8V1_000241"
     CDS             complement(39827..40306)
                     /locus_tag="ACG8V1_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0328369.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40312..41340)
                     /locus_tag="ACG8V1_000242"
     CDS             complement(40312..41340)
                     /locus_tag="ACG8V1_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0328370.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41357..42463)
                     /locus_tag="ACG8V1_000243"
     CDS             complement(41357..42463)
                     /locus_tag="ACG8V1_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0328371.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42692..45289)
                     /gene="adhE"
                     /locus_tag="ACG8V1_000244"
                     /gene_synonym="adhC"
     CDS             complement(42692..45289)
                     /gene="adhE"
                     /locus_tag="ACG8V1_000244"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0328372.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45594..45974)
                     /gene="yajC"
                     /locus_tag="ACG8V1_000245"
     CDS             complement(45594..45974)
                     /gene="yajC"
                     /locus_tag="ACG8V1_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0328373.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46071..47216)
                     /gene="tgt"
                     /locus_tag="ACG8V1_000246"
     CDS             complement(46071..47216)
                     /gene="tgt"
                     /locus_tag="ACG8V1_000246"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0328374.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47434..48132
                     /locus_tag="ACG8V1_000247"
     CDS             47434..48132
                     /locus_tag="ACG8V1_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0328375.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48300..50939)
                     /locus_tag="ACG8V1_000248"
     CDS             complement(48300..50939)
                     /locus_tag="ACG8V1_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0328376.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51558..52589)
                     /gene="queA"
                     /locus_tag="ACG8V1_000249"
     CDS             complement(51558..52589)
                     /gene="queA"
                     /locus_tag="ACG8V1_000249"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0328377.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52782..53956
                     /locus_tag="ACG8V1_000250"
                     /pseudo
     CDS             52782..53956
                     /locus_tag="ACG8V1_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            53960..54601
                     /locus_tag="ACG8V1_000251"
     CDS             53960..54601
                     /locus_tag="ACG8V1_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328378.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54598..55515
                     /locus_tag="ACG8V1_000252"
     CDS             54598..55515
                     /locus_tag="ACG8V1_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0328379.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55512..56183
                     /locus_tag="ACG8V1_000253"
     CDS             55512..56183
                     /locus_tag="ACG8V1_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328380.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56724..57212)
                     /locus_tag="ACG8V1_000254"
     CDS             complement(56724..57212)
                     /locus_tag="ACG8V1_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0328381.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57413..57679)
                     /locus_tag="ACG8V1_000255"
     CDS             complement(57413..57679)
                     /locus_tag="ACG8V1_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0328382.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57679..57942)
                     /locus_tag="ACG8V1_000256"
     CDS             complement(57679..57942)
                     /locus_tag="ACG8V1_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0328383.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58463..58603
                     /locus_tag="ACG8V1_000257"
                     /pseudo
     CDS             58463..58603
                     /locus_tag="ACG8V1_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(58745..60568)
                     /locus_tag="ACG8V1_000258"
     CDS             complement(58745..60568)
                     /locus_tag="ACG8V1_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0328384.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61166..63871)
                     /locus_tag="ACG8V1_000259"
     CDS             complement(61166..63871)
                     /locus_tag="ACG8V1_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0328385.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64177..65124)
                     /locus_tag="ACG8V1_000260"
     CDS             complement(64177..65124)
                     /locus_tag="ACG8V1_000260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0328386.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65314..65583
                     /gene="rpsN"
                     /locus_tag="ACG8V1_000261"
     CDS             65314..65583
                     /gene="rpsN"
                     /locus_tag="ACG8V1_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0328387.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65759..66121)
                     /locus_tag="ACG8V1_000262"
     CDS             complement(65759..66121)
                     /locus_tag="ACG8V1_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0328388.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66132..67253)
                     /gene="alr"
                     /locus_tag="ACG8V1_000263"
     CDS             complement(66132..67253)
                     /gene="alr"
                     /locus_tag="ACG8V1_000263"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0328389.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67269..67619)
                     /gene="acpS"
                     /locus_tag="ACG8V1_000264"
     CDS             complement(67269..67619)
                     /gene="acpS"
                     /locus_tag="ACG8V1_000264"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0328390.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67749..69260)
                     /locus_tag="ACG8V1_000265"
     CDS             complement(67749..69260)
                     /locus_tag="ACG8V1_000265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0328391.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69582..70934)
                     /gene="murF"
                     /locus_tag="ACG8V1_000266"
     CDS             complement(69582..70934)
                     /gene="murF"
                     /locus_tag="ACG8V1_000266"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0328392.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71087..72163)
                     /locus_tag="ACG8V1_000267"
     CDS             complement(71087..72163)
                     /locus_tag="ACG8V1_000267"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0328393.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72359..74104
                     /locus_tag="ACG8V1_000268"
     CDS             72359..74104
                     /locus_tag="ACG8V1_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0328394.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74384..74821)
                     /locus_tag="ACG8V1_000269"
     CDS             complement(74384..74821)
                     /locus_tag="ACG8V1_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0328395.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74805..76178)
                     /locus_tag="ACG8V1_000270"
     CDS             complement(74805..76178)
                     /locus_tag="ACG8V1_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328396.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76500..76988)
                     /locus_tag="ACG8V1_000271"
     CDS             complement(76500..76988)
                     /locus_tag="ACG8V1_000271"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0328397.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(77010..77612)
                     /locus_tag="ACG8V1_000272"
     CDS             complement(77010..77612)
                     /locus_tag="ACG8V1_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0328398.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22363 a        16548 c        14194 g        24551 t
ORIGIN      
        1 aaatgtaatc agatcattat ttttatatgt tgtaacttcc tgataagaaa actaattaaa
       61 agaaagaggt aactacataa actaattaaa aaagctaact tcatagttgg aggataaatg
      121 gaagaaactt caatgattgt ttctccttgt gttccttcaa tcgacactag tcctattatc
      181 aatctctctt ttttgtccat ttacttttgc gactgttccc caataatcaa atacaggaag
      241 cgttattttt tgtcctttag attcatgtac tgtatagact attttcgaat ccatcacttg
      301 ataaggcata gtatgccatt ccttgccaat tcttacttca tgattctgca aatgttcttt
      361 gtattgtaat gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga gtgtcgtgta
      421 acgaataaga ttttcttgat tcattaccaa gtgatctctc tgagtgacaa ctttatgttc
      481 atagtttgct tttgttgcag cagatacagc caacagtaat aaaacaattg ccgttatatt
      541 aactactttt ctttgcttag ctgatgtgct cttcatgaaa acgaacgata aaatcaacga
      601 gccaaacagt acttgaaagc ctaagattcg ccaaggaaat tgaatcattt gaatgggtgt
      661 attttgtaat aaagaccatg gaaaaaattt ggtggataag agccaagaga ctaccgcact
      721 aaggtaaatc actttttctt tgcgattaaa tcctttaaaa aagaagagtg gacagatcaa
      781 agaaataata ccaatgattc ctaccgtaaa ggttgtcagg tcattattca tcgcaccaat
      841 caaactatcc gagacattta atgcttggtg ttgtaaggtc gttttatctg gctcattaat
      901 cgattgaacc atgatttgtc ggaacatcgg ataccaaaag tatgctgtta aaagaatcgt
      961 cattcctact gcctttgaca actccacaat caaccggatg ctttcttttt ttcttgtttc
     1021 acttactaca aagccgccta agactagtaa gccaacggct attacactca taaaagtgga
     1081 aagaacatgt gtataaatga gtaagctcat actgactgct aatggaattg cacatggctt
     1141 tttctcataa atcagtgcgt aagcataata cataactatc ggtataaccg taagtgcaat
     1201 cgcttcagca atagcagaac gatgataaat atcaatcgta cggtacccat aaaatgtata
     1261 taaacagcta aatataaaag ccgcatcgat tctttttaaa aattttagtc cataatgaaa
     1321 catcagcatg atagtacaaa cattcaatag ccaaacatat aagatataac taacgattaa
     1381 attattactt atccaataaa aaatgactgc cggaaaaaag gtcaagtagg gataaaaata
     1441 attcactccg ctcccataat gattaaacgt cgtaaaattg attggaccag aaaaaattga
     1501 agataaccct ttgactctag caaaatggaa ggcaatatcc cccgatgtag aaatagtatg
     1561 aacatttcta aaataattga ttgaataaag tccagcaata attgctagga gaatggacaa
     1621 tattattttt ttctttactt gcattactta attatatact cctttcatgg attgctttat
     1681 ctagtataac ttaaatttta aataaatcgt tatttatttt tactctcact taaaaaagca
     1741 ctaaaattat aagagaggga ggtaatggtc tgaacaccaa gaattgttcc aacatcgaat
     1801 tgcttaacta atgagttcaa agcttaatta tgtggctctt atatttaccg agcgtggatt
     1861 gcgatctctt tagcgaaaca aacatctttc ataaaaaaaa cagccatatc cgctttcttg
     1921 gatatgacta tttactacta ctttactatc tttcattctt cttgtctcac ttatgataag
     1981 gcgcccccgc attgatccgg aaggcacggt agatttgctc gacaaggatc agacgcatca
     2041 attgatgtgg atacgtcatt tttccaaaag agatctgagc attgcttctt ttcatgacgg
     2101 cttcgctgag tcctaaagag ccgccgataa caaaaacaag gtggcttttt ccttggatac
     2161 ctaattggtc gatttgttta gcaaaagatt cacttgtagg atttttccct tcaattgcta
     2221 aggcatagac gtattcttgt tctttgattt tcgctaagat ccgttcccct tctttttctt
     2281 ttacttgtct catttgcgct tcgcttaaat tctccggggc tttttcgtca ggaacttcga
     2341 tcaattcgat ttttgcatag gcattcagcc gtttgacata ctcattgata ccttggatca
     2401 ggtatttttc ttttaatttt cctactgaga taattttgat attcatgttt tctctccttt
     2461 ggtcttcatg ctcactttgt ttattataat caattttttc gtctatatgt ttattttggc
     2521 tatttttgtt aaaaagattt ccccaacagt tatgcacaac atccacaggt ttatccacag
     2581 aaaacagtgt tattttacct tatttcatca cattcattag gcaaacatac gttttataag
     2641 aatttatcca ctttatacac aggctgtgga tatgttttat cccttatttt tccaatccta
     2701 tccgcgtagt tttacacagt atctgtggaa aaagaaaatc aaatattttc atctgaaaac
     2761 aatgattttt cctaaagttt ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa
     2821 caaataagac tatactaaca gtagataaat gaatgtaatt ggaggaggaa cacatggata
     2881 gaaaaaatgt gacaccaaag atgaaaaaaa gtaaaaataa tagcctctgg cgtaagttag
     2941 gtctggggct tgttggaggg atcgttggag gactactgac agctggcatt ttttatgcag
     3001 cgatgggcac tggcaacaat agttctactg ctacaagcgg caatcagaat tctgccggtg
     3061 aaacagttgt ggaaaatgtc aaagtcaatg tagattctga tatcaccaaa gcggtagata
     3121 aagttcaaga cgctgtcgtt tctgttatca acttacaaag tcaaaaccaa tcaagcggtt
     3181 ttggcggact attcgggcag caagaagaaa gtagcagctc agatgatagt aatttggaag
     3241 cttatagtga aggaagcggc gtgatctaca aaaaagacgg gaatactgct tatgtagtca
     3301 cgaataatca cgtagtagat ggccagcaag gtcttgaagt cttgatgaaa gacggtacga
     3361 aggtaaaagc tgaattagtt ggaacagatg cttattcaga tttagcggtg ttgaagatca
     3421 attcagacaa agtcgaaaca gttgcctctt ttggtgactc aagtgctctg aaggttggtg
     3481 agcctgcgat tgcgattggt tccccactag gatctgaata tgcaaattct gtcacttcag
     3541 gaatcatttc ttccttgaac cgacaagtga caagtacgaa tgaatcgaac gaaacagtca
     3601 atatcaatgc gatccaaaca gatgcagcaa tcaaccctgg aaattctggt ggtcctttag
     3661 ttaatatcga aggacaagtt atcggtatca actcaagtaa gatcgcaagt acttctgaat
     3721 ctacttctaa tgtaagcgta gaaggaatgg gatttgctat tccaagtaac gatgtagtga
     3781 atatcatcaa tcaactggaa aaagacggaa aagtgacacg tccagctcta ggtatcacaa
     3841 tggtcgactt atctgccgtt tctactcagc aacaagaaca aatcttgaag attccttcat
     3901 ctgtaactaa cggtgtcatc gtgacaaatg tccaaacagc tacaccggct gaaaaagctg
     3961 gattgaaaca atacgacgtc attactaaga tcgatgacaa agaagtttct tctggtgtag
     4021 aacttcaatc tgctttgtat aagaaaaaag tgggagattc tgtcaaaatc acttactatg
     4081 aaggctcgaa agaaaaaaca actactgttg aattgacgat cgatcaatcg gcattaaaac
     4141 aaaatcaaaa tagtgaaaat taataaaaaa gaggttgtga caaagacttg tcacaacctc
     4201 tttattttgg aaatgcttct ccgactacgg catcctcagg caatactaag attccttttt
     4261 ttgctggagc atttggcaaa tgcaattcac gagcggaaca gatcattcca tagctatcta
     4321 cccctcgcaa agttcctggc cagatcatca ttccatctgg catcatagct cctggttttg
     4381 caacgacaac tttttgtcca gcttcgatat tgggtgcacc gcaaacgatt tgcaatactg
     4441 taccattgtc tacttctgtc tcagtaatcg ataaatggtc agaatcagga tgtgggacgc
     4501 atgtttttac gtacccgaca acgatcttcg gttcatgatc agcttcaagc tgttcttcga
     4561 atcctgcttc tttgatcaat cgattcaatt tcgctaattc ctcatctgtt aagcttactt
     4621 ggccgttccc ttcgattgtt agatgatcgg agatatggaa aaagttccaa gcaacaactt
     4681 gaccttgttc atctttgatc cgagcaacat tccatttgcg ttcaacagtg ttttctgttc
     4741 ctttatcgtc ggctataata accatcaagg tgtctccgac gtgtgctttg ttataagcaa
     4801 aaatcatgtt ctttcctcca ttatcctttg ctgagattta tcttaacaaa aaattgcatt
     4861 cactgcatga aatttttttc atgctttatt tccgaagaag cctcttctgg aacaccattt
     4921 attcgtaaca agggactgtg acaagacttt tgtcacagtc cctttactgt tttagttaat
     4981 tgattaaaaa ggtcgtacaa gttccacttg cttaattact tggctgttct tcttcgtcag
     5041 aaacgatcaa gacatcacaa ggtgaatgac gtatcacgta gctggctaca cttcctgtca
     5101 taaagcgttc gactgcattt agacctgact gcccaaccat gattagatcg atctcatatt
     5161 tttttggcaa tttgactgcc atggattcac gaacagaacc atatgcaata attccttcta
     5221 tatttgtgta atcaacagat ttcccatatt ctttacattc ttcgatcatt tccttggctg
     5281 tttcagtttg ctgatcgact agattttgat caataggtgc atagcccata aagttataaa
     5341 attgctgatc gattacgttt gctacatata ctttcccatt attacggcga gccacttcta
     5401 ccgctttttt gaaagcagca agtgcttgtt cactaccgtc gatacctact aaaatattct
     5461 tatattcttg attcatcttg acccctccta acagatgtca gccttttgtt acactttcta
     5521 ggaacgattc gatttcctgt tttgttttac gatctttatt cactagacga cctaattctt
     5581 ttccgtcttg gatcacgaca aaacttggga taccaaaaat gttccattca gcggctacat
     5641 ctatgtactt gtcacggtct acttcaataa attgccaatc agaaaagtca ttttcaattt
     5701 caggcatttg cggtttgata aaacggcagt ctccgcacca gccggcagta aagaaaaata
     5761 cactctttcc agtttctacg tagcctgcta attcttctaa actatttggg ataatcattc
     5821 tttgtcctct actttctgtt tatgtatttt tcaattaaat tatacacctt tctttcgaac
     5881 taacaaacat tgatcctctt tttataaaaa aaatctggca aaaagcaatc ttttgccaga
     5941 ttttatagtt tcactatcgc attattttcc gtatacgatc gtatttactg ttgatttatc
     6001 caaacctttc agtgtttgaa tcaacatttc tcgtgcagct tcaaaatcgc gaatactgaa
     6061 catcgtttga tgcgtatgga tataacgtcc cactacgcca atcactgtac ttgggattcc
     6121 ttcgttttgt gtatgagcag cacctgcatc tgtaccgcct tttgaaacaa agtattgata
     6181 agggatattg tttgtttcag cgatatccag caagtactcg cgcaaacgag gaagcatgat
     6241 caaacctgga tcttggatac gcatcaatgt accttctcct aaatgtccga atgtgccatt
     6301 tttcgtcact gtatcatctg ctgcagagca gtctactgca aagaacagat ctggcttgaa
     6361 tttattgaca gaagctttag aaccacgcaa gcctacttct tcctggacat tcgcgcctgc
     6421 aatcaatgta tgacctaatg tttcattttg caacgcttct aaagcttcta ataccattgt
     6481 acagccatag cggttgtccc atgatttact gataatattc tttccgttgg ctgttttgat
     6541 cgtttcagcg taaggaacga ttgtgtctcc tggaaggaca ccgtaactca ttgcctcttc
     6601 tcttgattca aacccagcat cgaacaaaat atcggttact tctactgatt tttgtccgct
     6661 tgttcctcgc aataaatgtg ggggaacgga agaagaaata catggataat tcttaccagt
     6721 agaggtttta agtgtaaaac gctgagcaga aacaacgtaa ggattccagc cgcctagagg
     6781 tacgacttta aaaagcccgt tttcttggat ttgtgtcagc atgaatccga cttcatccat
     6841 atgtgctgcg accattacac ggggtgcatc cgcttcttca tgatggcgca gaccaaaaat
     6901 gccgcccaaa ccatctaact gtaactcatc taccagcggt tccatatgac tttccatata
     6961 agcgcgaaca tcttgttcaa aaccgcttgt tccttgtaat tctgttaact ctttgattcg
     7021 ttgaaatgtt ttttcttcca tatgctattc gtctaaattt cagactaaac tcctctcaga
     7081 cttatattga tctgcggata aaatatgatt ttttctgcag accgatcgta tttattatag
     7141 cttatttttt ctggtagcgg ttatttattt caaagaaaat atgagagtgt ggtaaaataa
     7201 gcacaaggag taacaagtga gaagaaggag gtatgtccac atgtatgaag aaagcgaact
     7261 aagctatttt aaaggcgggt tagcagttgg aatcggtcta ggattattga gcggggtagc
     7321 ttcaacgatt tggtatcatc gcaaaaaagt attgtcagca gaccaagtac ttgaaaatat
     7381 caagtcatct tttctaaaag aaggtgcaat cgaaggttct tggatctctt ttgaaaagga
     7441 acctattagg aaattcgccg ttcggtcgag tgcttatcgg ggaggtcttt ctcgaataga
     7501 agatggcgaa actgtttatt atgaattttt agcagatgct catacaggaa ccgtattaga
     7561 aatttcaaga aaaaaaggtg caacagatta atacgtcaaa aaagtgagag aacgaatacg
     7621 cacccactat tcaaaagaga ggtatcatgt ccttcttttg tagtgggtgg ttttttagat
     7681 agagctttcc aaacatatat tccagtattt tctttttaaa taaatgatat ttagcgggaa
     7741 aaactagcat ttcctttgat tatgaggtat ttttaattta gcttttgttt tctatagtta
     7801 aggagagtga aaaatggaac aacaagaaaa aattgctgtt ctgattcctt gttacaatga
     7861 ggaagcaaca atttcaacag tcattgcaga ctttaaacga gaattaccag aagcagatat
     7921 ttatgtctat gataataatt cgaccgatcg aacatacgaa ttggccgttc aaggtggagc
     7981 aatcgtaaaa aaagaacctc gtcaaggaaa aggaaatgtg atccgccaaa tgttctttga
     8041 tatcgatgcc gactattatt taatggtaga tggcgatgat acttatcctg cagaagctgt
     8101 acatggtttg ctcgagaaat tacgttctgg tgaagcagat atggtcattg gtgatcgcct
     8161 atcaaatgga acctactttg atgagaataa gagacctttc cacgattttg gaaataacct
     8221 tgttagaaat acaattacta gaatgtataa aacaaaaatt cttgatgtca tgaccggcta
     8281 tcgaggattc aatcgtatct ttgtcaaaag ttttcctatt atgagttctg gttttcaaat
     8341 cgaaacggaa ttgacgatcc atgctttaga taaaaaattc aagttcgttg aaatcccaat
     8401 cgattatcgt gatcgtcctg aaggcagcga atcaaaattg aatacattct ctgacggatt
     8461 caaagtgatc atgatgatta ttaaaatgtt taaagattat aagcctttgc ttttctttgg
     8521 gatttggacc atcttcttct ttttatttgg attggtcacg gggattcctg tgattcgcga
     8581 atatatgctg acaagcttta ttacacgcat cccttctgcg attttaagta ctggattgat
     8641 gactttagca cttctttcat tagtaacagg gttgattttg gatacagtcg tcacgaacgc
     8701 taagaaagaa tatgaattga atctatatca tgtttatcat gaataccaca gtagaaagta
     8761 aaaaaaggat caagactgca aaaataattt gcaattcttg atcctctttt taattaatta
     8821 tttcgccaaa cgatagatgg catctgcata gattgctgtt gctctgaaca agtcatcaag
     8881 actcataaat tcgtttgctt gatgcattgt atcagtatat cctgggaaca tcgctccata
     8941 agcaacgcca cgtttcaaca aacgtccgta agttccgcca ccgatgattt gttcgtagcc
     9001 tttttctcca gtgtggtctt catagacttg caataatgta gccaccaatg gatcatccat
     9061 tggtacgtaa tgtggttcca ttacgcgagc acctcgagta actgttgcgc cttcttgccc
     9121 cactgtttgt tcgatgtcac tttgcaatcc gtctactgtt acaccttttg gaaaacggaa
     9181 attcatattg atgaagttcg cttcctctgt accattttct tcaaaagcaa atagtccagc
     9241 attcattgtc aatttgccca ttttttcatc ttcataagcg actcctagtt tttctccata
     9301 aaaatcttca tggacataag cagctgctac atggataaat tgtttagcag atcctaggaa
     9361 ttcatagtta tctaagaaca atgccaagaa agaacctgca ttgataccag attgtgggct
     9421 tgcaccatgt gcgcctttac caactaattc gatttttaca tacgtattgt ccgcttcaat
     9481 cgttccgctt actggatttg cttcgatgaa ttggtaaaat gcttcttcca tcgcgatagc
     9541 tgcatctgca cttggtactt gaagtgctgc agttgctgtt cctggcacca tattttcgcg
     9601 aagtccggaa acaaaagatt tcaagacata atctcctgca ttgtcgcctt tgaagcgtaa
     9661 agctaaagaa acatttcctt tttcaccatt gatgattggg aattcagcat ctggtgagaa
     9721 accaaaatct ggttcttctt catgttcgaa ataataagcc atatcgcccc agccgctttc
     9781 ttcatcgctg ccgactacga agcgaacttt ttttgatagt ttcaagtcaa gttctttaat
     9841 gatcttcatc gcatagtaag ctgccatact tggacctttg tcatcgcttg atccgcgtgc
     9901 aaagatcttt ccatccttga ttactggttc ataaggatct gtgtcccagc catctcctgc
     9961 aggaacgaca tccatgtgtc caaatatccc tagcgtttcg tctccttcgc caagatcaat
    10021 atgccctgca tagttatcga cattttttac tttaaatcca tcacgttcgc cataagctaa
    10081 catatgtttc aatgcgtcgc gaggtccagg tccaaatggt gcatctggtg ttacttttga
    10141 gtcatcgcgc tcgctgttga cacgtaaaag atttttcaag tcttcaagca agtcttcttt
    10201 acgtgcttct acttcttttt gccaatcaat tgtcataaag ttaatcctcc acttatttaa
    10261 gtcttcgtgt tcatgatacc acaaattaca gaaaaaaata gttcaaaaaa tctgacaatt
    10321 ccttttctta tctgtttttc tctaaaaaaa atatacgcta taatgagtaa aaggaggttt
    10381 ttagatgctg ataaacgaac aactagtgaa aagagtcatc acacctcgtc caaaaatttc
    10441 acataaagga acatttggac gtatcgtttt gattggcggc aatgaacaat atggcggagc
    10501 aatcattatg agtgctttag ctgccgtaca cagcggagcg ggtctcacga ctgtagtaac
    10561 tgatgaaaaa aatcggagtg ctcttcacag tcatctgcca gaagcaatga ttgtagtttg
    10621 gacggatact gaccagtttc tttccgtaat aaagcaagca gatgtactct tgattggtcc
    10681 cggattgggt acttcttcta agagtttgga acgtttcaaa caagtactag aaaaccaatc
    10741 aaaagaacaa tggctggtga ttgatggctc tgctttaagt ttacttgctg ctaatcatct
    10801 tttgctaccc tatcccgaac aaactgtact tacacctcat caaatggagt ggcaacggtt
    10861 gagttctcta ccgatacaag aacaacggga taaagaaaac aaaaagaagc aacaggaaat
    10921 cggtgctgtg ctcgttttga aaagtcatga gacaaaaatt tatggtcacc agcacttcta
    10981 caccaatcct ttaggttctg ctgctatggc aacgggcggc atgggtgaca ctttagctgg
    11041 gatgatcaca ggatttttag cccagtttcc tcaaaatgac caaacgattg cagcagctat
    11101 ttatcttcat agtttcattg gagaccagct ggcaaaagaa caatatattg ttttgccgac
    11161 acaaatcagc gaagcacttc cgaaatggat gaattatttt tgtaaaaaat caatggaaaa
    11221 cgaggcatga gtcatggaga aatttacagg ttatgtgcga gaaccttttt attatgaaac
    11281 agatcaaatg gggattattc atcacagcaa ttatatccgc tggtttgaag aggctcgggt
    11341 agccttattg gtacatctag ggtttcccta taaaaaaatc gaagaatctg ggatcatcat
    11401 ccccgttctg gaagtaaatt gcagctataa agaaatgatc cattacggag aaaaagtgag
    11461 catccatcca actatccaaa agtatacggg gacacggttg gattttagct acgaaattgt
    11521 gggaactgaa gacaaaaaat taaaaactac tggcagtagt aaacactgtt ttttactttc
    11581 tgattcgcaa cgattagtga agctgagcaa agtcaatccg gagttagatc agttattcca
    11641 agcgtacgct tctttggatg atgtaaattc ttaataaaga ccgtctaagc atttttcttg
    11701 tattgtgtcg agtagttcgt ataatgaaaa gaaaaaagca ggtgatcccc atgtatgtcg
    11761 ttaaagtaat gcacggttac atcgataaaa ctggctgtcg cactcgggaa aaaaacttag
    11821 acaatctgct cattttcaaa gacaaaaaag aatccgaagc ttttgctaaa agaatcggcg
    11881 gtcgtgtcaa accgatccaa gaggtacgac cagattgatt caatattttt cttttcgagg
    11941 ctctaatagg catgcctatc agggcttttt ttgcagactg tgtttttatg cccacacgat
    12001 ttgtaagcga tttatgttat aatagattat atctgcatag aaaggaaggt taaatggtta
    12061 tcaataattt gcaacagcaa gcttatgaat ctatacgtaa acaaatcatt tattccgagt
    12121 tagcacctgg aagtaaaatc tccgataaag tgttagaaga acaactagct atcgggcgta
    12181 caccgattca agaagcactg atccaactgc gcaatcaaga gcttgtaaaa accattccac
    12241 agtccggcac ttatgtttca aaaatcgata tccgatctgc ttcattagcc cgttatacgc
    12301 gggaaaagtt ggaaaaaccg atcctgcaag aatgcagtgc gaaaatgacc aagcaagatc
    12361 aagctaaact agaaaaaata ctagaacaaa ccgatcaggc agtaatcgct caaaataaaa
    12421 aagctttttt tgatttggat actgattttc accgaacttg ttatgaaatt gctggaaaac
    12481 gagaaatttg ggattggcta gaaagttata gtacgcactt gaaccgtttc agatggttac
    12541 gtttgacgat ttcagaatta gactggggtc gagtattaga tgaacatcag acaatgcttc
    12601 aatcaatgat tgatcataac tttgatgaag tcggatttct ttgcacgatg catttacata
    12661 tgattatcga ggagcaagaa tacgtgattc ataattaccc cgattatttt gaagatattc
    12721 aggacagacc catcaaataa gaggttttgg aatcagcgtt ttgacctgag tattagagaa
    12781 agaaacggca aaatagttcc tcatattttg ccgttttttt tgagctaata ccgagggtca
    12841 gctgattgca caccgtttat tgagaggttg tgaaaaagct gccctgcatc aagtaataag
    12901 aacagccaag taaaaatagc ttcttatgtt tttaacttgg ctggacttat taccgcagat
    12961 gcaagctttt gaacaccgtt ttccaagagg tcgtgagcct gccgttcatc tccgagtcac
    13021 aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa tattttgact tgtgaccgag
    13081 gagttgacag gagaacatcg tttattcgga aaaaaaggac tgtgacacga cttttgtcac
    13141 agtctcttta tttgtaatct aaatcatact ccgtacatct tttttctatt ttttttattt
    13201 cttctaagat cccttggcta cgaatttcat tatatggcca aagtaagact gcctctaata
    13261 cataaggcga aaaatcgctt tgattcatct cttttgaaaa actcatcgct gctatctcgc
    13321 tcaatgcacg aatgtttgat tgatgtgtat aaaaaggcaa ccgccacagc acaatttttt
    13381 cgctttgcgt ataaattccc ttaacagaag cttcgatgtg gtgaaatagt tcatgtccaa
    13441 taatcaaagc ttgcgctgtt tctttagaga tcgaagctag tcctgaacat tttaattttt
    13501 ctaacggctc cgtcatcaat cgaatccctt catcttctgc ataagttgca aaagtgattc
    13561 gttcgtttat ttgctctgat tcacaggaaa cgattggaac agccatttgt tcacaaattt
    13621 ctcctacatc ggcaaaaggg tacttttttt tgatgctaca tgccgtttcg ataccacaat
    13681 cgattgattg ttgtataaga attttctgct tggacaaagg aatctttttc caaaataaat
    13741 ctcgccgaaa ctggtagaat ccccaaattt cttcggggat ctgttgtaat tcgtttagta
    13801 actcttctaa cttatgtttg tttgatattt ccggcaacga cgatcacttc ttcctccggt
    13861 tccatagtag atacttccag atccatcaaa agctgtagtt gttccagatc cgtcgctgta
    13921 aaatcagaga ctcttgctcc taagcagaga taaaattctg gtcgggcaat caattcagtt
    13981 tgataaactg ccatatcttc ccaaagttgt tcgacagctg tcatataatt ggctgcagtt
    14041 gttttcatac atgaagcatg tccacgttgg accttgataa atcctttttg atcgatgatc
    14101 cgaagacttc cagcattttt tgaacgattc tgttcgccgc atacataaaa gaaaggtgta
    14161 ttttccaata agcaaacttc atttttattt aatcgcatat cctctgcagc aagctctagt
    14221 gcttcttctg ttgtgatctc cttggttaga tcggttgctt tcacttccgt agaaccagta
    14281 gcgatagccg ttacttttga agtctgtgga tcgatttcta cgtggacttc gatcgattct
    14341 ggtgtagcgc ctgattcaat cgctttattc atcgcttcat ttttcaacga tcgtatatct
    14401 tctttacttg gcgaagggat gatacgttct acaacatctc ggaccattgc aagtgctaca
    14461 ccaatagaag aaatcacttc ggcgttttca ggaatcgaat atttcacacc cattttatta
    14521 ctaaagtaag taatcaatga ggcagcgccg ccccctacac ctacaagaga aatctgatct
    14581 ttctccaagt gatacttgtc tgccaattct aaaatcaccg gttcaatctt agcataggat
    14641 ttttccatga tctgttctgc gatgtcttca accgtcgtat gacaataatc agctaatgct
    14701 tggatggctt ttcgtgcaga tgggacattt ccataagaaa aatgttcttc ttgcactaat
    14761 ccaaggacat tggctgcaca tgtatttgta atagtcactt cttctccgtc ttccatcact
    14821 accttgacgt aatcagcagg gtctccttct ttaggagaga aaaattcaac cttaggacct
    14881 ttgatttttt ctgtctccgt aaatactgca taatctagac cggcaatatg tgcagaacgt
    14941 ggaccgacat cgatgattcc tgactgatta gcccgtacca tcgaaccgcc tgccacacct
    15001 agaacccgaa catctaatga agagatatat gttgggtggc cacctacaat tgaataatcg
    15061 attgctggac gtccattttt aatgaccccg atattggttg ttgttccgcc tacttcaaaa
    15121 taaacaccat tagacgctct caagtacatc aaagagccca tgactgatgc tgctggaccg
    15181 gaaagcatgg ttaaaacagg tcgttttttc atttcattga tttccattac tccgccgtct
    15241 ccgcgcataa tcatcaaaga aacattgacc cctgcttctc gtacggaatc ttctgtagag
    15301 gttgccgtat ctagcatttt tggtaaaata gatgcattga ttgctgctgt ccgagttctt
    15361 cttgtcaatc catataattt tgtgatgtca gaagccatcg ttgtagggat cgattttacc
    15421 gatgctgctt cataaacaac tcgttctggt tccccattat ctactccaaa tgccatagaa
    15481 gaaaccaaga cctccgctct ttctctttct aaagaagaaa tcgtttctga aacacggtct
    15541 acatttaagt gtttgacagg taagaatgca ttgacaatct ctattttttt cttattccct
    15601 aaatcgatat cgttcaatct agtttgtcgt tttgctaaaa aaccttctaa tccacctttt
    15661 gccatcccga ttacgccgac ttttgctacg tctccttcaa tcagtgcatt agtcgcttgt
    15721 gtggtagaat gtgcaacaaa cacaacatct tctggactga tattattttc cagcaaacaa
    15781 ttttgaaaag actgaacgac acctgcggct actccacgga catcatcgtg ggttgttttc
    15841 actgaagatt ttccgatgat ttcgtgcgtc gcattatcga ttgcgactgc tttcgtatgt
    15901 gtaccgccaa catcaattcc cattcgcaca aaacgtttca ttttcttttc ctccattcat
    15961 ctaatcgttt ttatcgacgt tttgtttact aggcgttccc gtacaataag aacgtgatca
    16021 ggcataagat cgctccgatg atccaaccag ttgggatgga tttcttcata tattctcgag
    16081 aagttacttt tgtataacct aatccccaag caacccaaga ttgtgtaata tctaaatgct
    16141 gaggagcaat cgttgtgatt gcaaataacg ggaacaaaaa ggctggcgac atcgttggat
    16201 taacagctaa tacgacagct aaaatagctg agcctgaacc gactagattc attggaccgc
    16261 ggaaaaatcc tagtggtgtc aatatagcaa atacgattgc caagactaat ggtgattgcg
    16321 gtatccaatc tccaaaaata gctgaaaaat aaggagaagc atacgcagct gaattattga
    16381 acatcgctaa ggtcatcaag aaaccaacca ttggcgcaac atctactgcg ccatctgtaa
    16441 ataatttcgc aaactgacga cagatttctt tataacctcc gcgcaatttt ccagtaaaca
    16501 gcaatgccca gatacctgct agaataaaac ctaagatgat tggaagatct aaaagaacga
    16561 cccctaaaac tggtaataat acggctaacc atgagatcat tggtgcgtcg ccaccttcag
    16621 ctggtacgtt tgctgccatt gcatagcgtt tctttttgtt cattgatata ttagcgaccg
    16681 ttagtaccac aactaagctt acaatcatcc caatcatacc aatttggaaa tagtcattat
    16741 aagtatagct ttcagccgct ggattgaaac cagcataaat cgtttgatat tgtttgaaat
    16801 tgacgatatt agcaaagatt ccagccataa tcgagcccat aaatgagaac agtgcaaccg
    16861 gtactgaaat acctaatgac atcaagattg gcaacacaat aactgcaata gaaatcactg
    16921 gtccgattcc tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg
    16981 tgattcttgg cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc
    17041 cgctatctac tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt
    17101 tggcgtactc agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc
    17161 cttccatcgc cgggtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag
    17221 tagccatgat ggcaaagccg accatcaagt tgtagccttt tacacagtac cagaccatga
    17281 aaaagaatga gataactaac aaaataccaa taataatatc actcatgtta ttcctccgtc
    17341 cattttgttt ttctgataag caaactaata tatcagtcaa actcattcta acttatgtag
    17401 ttagcgcttt cattaaaggg tgaccataaa tgttcagttt tcaattaaaa aaccttaatt
    17461 atgctatttt agacttatac gttattgtcc tgtttcattt gatggaggat tgtaaataaa
    17521 aaaagaggaa agaacataac ttttcgttat cttctttcct ctttataatt gtttagctta
    17581 tttagccaaa gcttcttttg cttggtcagc caatgcagta aatgctgctg catcgttaac
    17641 agccaagtca gctaacattt tacggttgat gtcgatttca gcaactttca aaccgtgcat
    17701 taattttgag tagctcaagc cattcatacg agctgctgcg ttgatacgag caatccacaa
    17761 tttacggaag tcgcgtttct tttgacgacg atcgcgatat gcgtagttgt atgaattcat
    17821 tacttgttct tttgctgttt taaataaagt gtgttttgaa ccgtagtaac cttttgctaa
    17881 ttttagcact cttttacgac gtttacgagt tactgttcca cctttaacac gtgccatgta
    17941 taattcctcc taataaaaat aaatcttcag attctgttat ttgtacttct tatttcattt
    18001 ttgctagttg ttggcgaata cgtttgaagt cgccactaga aaccatgctt gctttacgca
    18061 attgacggcg ttgttttttt gttttgccgt ggaaacggtg acttgtaaac gcgcggaagc
    18121 gtttcaatcc gcctttacca gtacgtttga aacgttttgc tgatccgcgg tgtgtttttt
    18181 gttttggcat gactatattt cctcctcaaa atctttcgtt atccgactat cggctgtcca
    18241 acagccagtt agtcattcaa attacttact gtcgtttttc ggtgccagcg tcaagaacat
    18301 gctgcgtccg tccattttcg ctttttgttc cactgttgca atatccgcag tttcttcagc
    18361 taagcgattc aagacttttt gaccaatttc tttatgggta atggcacggc ctttgaaacg
    18421 gatagaagct ttcactttat cgcccttttc caagaactta cgtgcattac gaagttttgt
    18481 attgaagtca ttcaagtcga tcgtaggact taagcgaact tcttttacat tgatcacttt
    18541 ttgtttttta cgcgcttcgc ggtctttctt ttgttgttcg aaacggtatt taccgtagtc
    18601 catgattcgc gcaactggtg gtttcgcacc aggggcaact aacacaagat ccaagtttgc
    18661 ttgttctgca atattcaatg cttctgcttt tgtcttaact cctaattgct caccatctga
    18721 accgatcaat cgtaactctc gtgcacgaat gccgtcgtta accatcatat cctttgctat
    18781 ggtcattcac ctccaaatat tatgagagaa agaaaagctt accgaataaa cggcaataaa
    18841 aaaacgagtt ctcttacaga acccgcgcgt acttcaccta atagaaccag tgaaaactat
    18901 ctagcccagt gacattatca actaaggcga gaagcgggtg cttctgcttt tatttctcaa
    18961 ccatcatagt atagcaaaaa ctaatctttt tgtcaatgtt tttcatacag tttgtcttcc
    19021 ttttctgaaa atgattagat tagcagactt tcttgcagtg attcccgctt ctttgctata
    19081 ctaaagaaaa acgaggtgaa cccacatgaa aaaaatcgaa cctattccac cttctcttcc
    19141 tcttcttaca aagggagagt tttatttaga agatgaggag atcttctctg gtcttctgat
    19201 caacgggggt gaccaaagct atctcgatgc caaaaatatg gtattaagag agagtcatat
    19261 ccaaaaactt acgatgcaaa aaacagtttt acgtcatttt gaatgcagta atgtcatttt
    19321 tgaaaaatgt gatttttcca atctggagtg gattggtggc agtttccacc aagtcgtgtt
    19381 tcatcaatgt aaattaacag gtacgaattt tgccgaaagt tatcttcgag actgtacttt
    19441 cactgattgt attgcttcga tggcttcctt tagtagtaca aacttgaaag ctgtctcctt
    19501 cgatcattgt caattagaag acagcgaatt ttatgaggtc acttggaaaa accttttttt
    19561 gtctgacaat cagttgaccg gttcgaactg gtttcgaact tctctgaagg gattagactt
    19621 tacgactaat ttcttcacag caatcgcttt atctcaagat tctttggcgg gactaatcgt
    19681 caaccaagag caagcacttg tgatcgccca agcccttggt ttgcagatca aagactagat
    19741 caacgaatcc gccgccacag tgcaataagc atccaaatag ataagaccaa actgatgatc
    19801 aaggcgattc cccatccgaa aacggaatgg gtaaagggta actggagatt cattccgaag
    19861 aatcctgtca caatagttgg aacggtcaag atcagcgacc agaccgtcaa aaatttcatc
    19921 gtgtcattca agttgttatt cagcaaatta ttataagtgc cagagagctg gtctaaaatc
    19981 tgcgaagcta agttcgtcat ttcaactgct tgtcgtgctt cgatcagcgc atcgtccaac
    20041 tcttcttttt ccttttctga caacttgcga taaatagcca atgccttgat ttgttctaaa
    20101 agtactacat tttgttttgt tcctgtaact aagtaaacca aaccgatctc taaatctgaa
    20161 agttgcagca gattcttgtt cgttgttttt tctcttaatt tcagattaag ccgctgacgt
    20221 tcactattca cttcctcgat taaaggaaaa aaggaatcag aaatcaaaaa caatgtacga
    20281 aacaataaac tgtatacact catgtctggc attcgttcca aaagcgatcg aattagtcga
    20341 atcacgtact cggtattcgg cgtggtaaat gtaaacaaac cgtcttcttt taaaataaaa
    20401 gcgataggac tagtttcata atgattatcg actttttgct ggtgcggaac atttgataca
    20461 agcagtaacg cttcatcaaa agaatcgtac tctacgcgcg cccgttcatt tttatctaga
    20521 gaataagtca gcatctcgtc actaatgcca tattcttctt ttaagttttt aacagctagc
    20581 acatcgtttt gctgtacatt gatccaaaaa ttacggtctt gattaaaata gtgtttttcc
    20641 cgcatactct atcctcctga catttgtttc cagtataaag aaaaaagtcg ctcaaataaa
    20701 ggtttaccct ttatttgagc aactgtaaat caaatggcta ctttggtttg gcgaatctct
    20761 gctaattttt cttcgatcat tttcaagact tcttcttttg cttgaggatc ttccacaaaa
    20821 ttatagcggt ctccatcgat ttggattttt ggactttctt tgtattcttc ataccattga
    20881 tcgtaacgtc tgttcaattc tttgtaataa tcgtatagac ttggatcaaa ggacaattgt
    20941 tcatacgaac gaccacgttt ttcgatacgc tccaacatcg tatcaaaaga tacacgaata
    21001 tgaactaata aatccggatg ttttttatgg gcagcatagg gaagttcttc catcatgttt
    21061 tccagcagtt catcatacac acgaacttct gtttctgttg ctcttcctag atctgcattt
    21121 agatggaaaa gcagtgaatc ttcataaatc gaacggtcca atacattatt gtcatctttc
    21181 attgcttgtt tgatgcttgc aaaacgttta ttcaaaaaat aaatttgcag caaaaaagca
    21241 tactgttcag ggtttgcata aaataagggc aagacttcat tatcatcaat cgattcataa
    21301 aatgcctgac tatcaaagtg ttcagccatc atttctgtta aacttgattt tccggcacca
    21361 attgtacctg ctaaaacgat cacgctcatt tacctccagc tcacttcatc actaaatatt
    21421 gtgtttaact ccaacctttt taaatatacc cccaatatct agtagtgtca attgtatttt
    21481 tttctagtca aagaagaaaa aaagtcaaaa aattcttaag aaaaaagtgt tttttcttta
    21541 gaaaacgtat tcttagtttt acttctgaga tggttttagt aaaataaagg aggaatgttt
    21601 tttgagaccc ttagtggaca aaaaaattcg tgaagcagcg caactcgttt tgggcgcttt
    21661 attttttgct taaaaatcac ttttacgtga gaaggagtta gattatggca aactatggcc
    21721 aaggagaatc aagcggaaag atcatattga tgggcgagca cgcggttgtt tatggagaac
    21781 cagcgattgc ctttcctttc tatgcaacaa aagtcaccgc attccttgaa gagctggatg
    21841 caatggacga tcaactggtt tcttcctact attcaggaaa tttagccgaa gctcctcatg
    21901 cattaaaaaa tatcaaaaaa ttattcattc acttaaaaaa acagcatgac atccaaaaaa
    21961 acttgcaact gaccattgaa agcacgattc ctgctgaacg tggaatggga tcaagcgctg
    22021 cagtcgccac agcagtcact cgtgcttttt atgattactt agcatttcct ttgtctcgtg
    22081 aaatactatt agaaaatgtc cagctttcgg aaaaaatcgc ccatggtaat cctagtggaa
    22141 tcgatgcagc cgctactagc agcttgcagc cgatttattt tacaaaaggg catcctttcg
    22201 actacttttc tttgaacatc gatgcttttt tgattgtcgc tgatacagga atcaaaggac
    22261 aaacaagaga agccgtcaaa gatgtcgctc acctctttga aacccagcct catgaaactg
    22321 gacaaatgat ccaaaaatta ggatacttga cgaagcaagc aaaacaagca atcattgaaa
    22381 attcaccaga aacgttggca cagacaatgg atgaatcaca atcacttctg gaaaagctga
    22441 caatcagcaa tgattttctt aatctattga tccaaacagc aaaagatacc ggggcattgg
    22501 gtgccaaatt aactggcggt ggacgcggtg gctgtatgat tgcattagca caaacaaaaa
    22561 caaaagccca agaaatcagt caagcacttg aagatgcggg tgctgctgaa acttggatac
    22621 aaggattagg agtacatacc tatgtttaaa ggcaaagcac gcgcatatac caacattgct
    22681 ctaatcaaat attggggtaa gaaaaatgaa gaactcatcc ttccaatgaa caacagcctt
    22741 tcgttaacat tggatgcatt ttatacagag acagaagtca tcttttctga tagctatatg
    22801 gtagatgaat tttatctaga tggcaccttg caagacgaaa aagcaacaaa aaaagtcagt
    22861 cagtttcttg acctttttcg taaagaagcc ggcctctcac taaaagcttc agtaatcagt
    22921 cagaatttcg ttcctaccgc agctggatta gcctcctctg ccagcgggct agctgcttta
    22981 gcaggcgctt gcaatactgc tcttaagctt ggattagatg atctctctct ttcaagattt
    23041 gctcgacgcg ggtctggttc agcttgccga agtattttcg gtggtttcgt cgaatgggaa
    23101 aaaggccatg acgacttaag ttcttacgct aagccagtcc cttccgattc tttcgaagac
    23161 gatttagcaa tggttttcgt tttgatcaac gaccagaaaa aagaagtgtc cagcagaaat
    23221 gggatgcgtc ggacagtcga aacatccagt ttttatcaag gctggttaga ttccgttgaa
    23281 ggggatctat atcaattgaa acaagcaatc aaaacaaaag atttccaact tctcggagaa
    23341 acgatggaaa gaaacggact aaaaatgcat ggaacgactt tagctgccca gcctccattc
    23401 acttactggt ctcctgacag cttaaaagcg atggatgcgg tgcgacaatt gagaaagcaa
    23461 ggcattccat gctactttac gatggatgct ggccctaatg tcaaagtttt agtggaaaac
    23521 agccatttat ctgaggtaca agaaacattt accaaattat ttagcaaaga acaagtaatt
    23581 actgcacatg ctggtccagg aattgcgatt attgaataaa gggagatttt tatgattgaa
    23641 gtatctgcac caggcaaact ctatattgcc ggagaatatg cagttgttga aacaggccat
    23701 ccagcagtta tcgctgcagt cgatcaattc gtgacagtca ctgtagaatc tgcacgaaaa
    23761 gtcggaagta tccaatctgc acaatatagt gggatgcctg tacgttggac aagacgcaac
    23821 ggagaattag ttttagatat tcgggaaaat ccttttcatt atatccttgc tgctattcgc
    23881 ttgactgaaa agtatgcaca agaaaaaaac attcttttat ccttttatga tctgaaagta
    23941 acaagtgagt tagacagttc aaatggccgg aaatatggat tgggttctag cggtgccgtg
    24001 accgttgcaa cggtcaaagc cttgaatgtt ttttacgcgt tgaatttatc tcagttggag
    24061 attttcaaga ttgcagcact agccaattta gcagttcaag ataatggttc ctgcggcgac
    24121 atcgctgcta gctgttatgg tggctggatc gctttctcaa ccttcgacca tccttggctc
    24181 caagaacaag aaactcagca ttctatcagt gagttacttg ccctggattg gccaggtcta
    24241 tccattgagc cattgattgc tcctgaagat ttacgtttat tgattggttg gacgggtagc
    24301 cctgcctcta cttctgattt ggtcgatcaa gttcaccgtt cgagagaaga taaaatggtg
    24361 gcttatcagc ttttcttaaa aaatagtaca gaatgtgtca acgaaatgat caaagggttt
    24421 aaagaaaata atgtaacgtt gatccaacag atgattcgaa aaaaccgaca attactgcat
    24481 gatttatctg caatcactgg ggtcgtcatc gaaacgcctg ctttgaacaa attgtgtaat
    24541 ttagctgaac agtatgaagg agccgcaaaa tcttctggtg caggtggggg cgattgcgga
    24601 atcgtaattg ttgaccagaa atctggcatt cttcctttaa tgagtgcatg ggaaaaagca
    24661 gaaatcactc cactgccgtt acatgtctat agcgatcaaa gaaaggaaaa ccgatgaatc
    24721 gaaaagacga acatgtttca ctagctaaag cctttcatga taaacaaaaa aacgaatttg
    24781 acttcgtacg gatcatccat aaccctttgc cgcaaatagc agtttctgat gtcgatctaa
    24841 gtacacaggc agttgggttt acactaagca gcccatttta tatcaatgcg atgactggcg
    24901 gaagcgaaaa aacaaaaaaa atcaaccaag atcttgcaat cgtcgcaaga gaagcagatt
    24961 tgatgattgc cactggttca gtcagcgctg ccttgaaagt cccttcttta gccgatactt
    25021 atacgattat gcgacaagaa tatcctcacg ggaaaatcat tgccaacatc ggtgcaggga
    25081 cttctgtcga aagagccaaa gaagctatcc gattgtttca cgcagatgcc ttacagattc
    25141 atttgaatgc acctcaagag ttggttatgc ctgagggaga ccgtgatttt accaattgga
    25201 aagtacttat tcaagaaact caaacagcca ttgacgtccc cctcatcgta aaagaagtcg
    25261 gttttggcat gacaagagaa acgctcaatg atctagctgc tttaggagtt catacagttg
    25321 atatcagcgg tcgaagcgga acaagcttca cccagatcga aaacgcccgt cgctccaagc
    25381 gggaactgag ttatttagct gactgggggc aatcgacagt ctcttcattg cttgaagcaa
    25441 atgaagcaga cacttctatg gaaatcctag cctccggagg tatccgcaat gcctatgaca
    25501 tatttaaagc cctttgtctc ggcgcaaatg ccgtgggtac ttcaggaacc gtactgacgc
    25561 atctgatgaa tcacggcgta gaagaaacta ttatactgat gaagcaatgg caagaagagc
    25621 ttcgtctgct ttatactatg gtcggcgcaa cgaataccgc ggccctacac caacaatctc
    25681 tgatcttttc cggaccagtg aaggattggt gcgaagcaag agggatcgac ttagtgaagt
    25741 atggaagacg cacagaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc
    25801 taaacacgta caaacggcgg aagtaaaagc aactccttcg gaaataagct gaaattcaca
    25861 aaaatttgaa aagcaatttt cgtgaattcc ttcttatttc ttggagttaa atgcttctgt
    25921 cccagcctct tttttcgaat aaatgatgtt taatcagctg tccttcggta ttcgttcaat
    25981 ccgctgattt cacaacctct ccactaaaca gtgtactaaa gctcttttaa ggttgttttc
    26041 cgatataggc aagtatgcct ccgtccacat acaagacatg gccgttaacg aaatcagatg
    26101 cgctagaagc caagaagaca gatggtccag ccaaatcgat tggctcgccc cagcgtgctg
    26161 ctggcgtacg tccaacgata aattgatcga atggatgacg ttcaccgttt tcttgtgttt
    26221 cgcgtaatgg agcagtttgc ggtgtagcaa tataacctgg tccaattcca ttacattgga
    26281 tattgtattg accgtattca gaagcgatgt ttttcgtcag catttttaat ccgccttttg
    26341 ccgcagcata agcactgacc gtttcgcgtc ctaattcact catcatggag cagatattga
    26401 tgattttacc gccgcctttt tcgatcatat ccggaataac tgcttttgaa acgatgaatg
    26461 gggcatttaa atccacgtcg attacttggc ggaagtctgc cgcatccatt tcgatcatcg
    26521 gtgtccgttt gatgatccct gcattgttga ctagaatatc aactgaacct acatcttctt
    26581 tgatctgttg aaccatcgct tgaaccgctt cttcatcggt cacatcgcag acataacctt
    26641 ttgcatcgat gcctgcttct ttataatgcg ctataccttc atcaactgat tcttgattca
    26701 agttattaaa tacgattttt gctcctgctg tagctaaaga gcgtgcaatc tcaaatccaa
    26761 taccatagac agcaccggtg actaatgcta cttttccatc taaacgaaac atatccatac
    26821 taaattccat cattttctct cccttatgct tattttaatt gatccatttg caccatatcc
    26881 atgtctgtat aggtgatatt ttctccgcac attgcccaga tgaatgagta attgcttgta
    26941 cctacacctg aatggatcga ccagcttggc gaaataatgg cttgttcatt gtctacaact
    27001 aaatgttttg tttcatctgg tttgcccatc atgtggaaca cacgtgtatc ttcattggca
    27061 taatcaaagt agacataagc ttccatacga cgttcatgtg tatgacaagg catcgtattc
    27121 caagcgcttc ctggttctaa aatcgtataa cccatttgta attgacagct ttcgcataca
    27181 tttggatgga tgtattggta gattttacgt tcattcagtg tcaggccttc accagtctcc
    27241 attggtttta cttggtcgat actgattttt acatttggat atttatgatg agctggaaca
    27301 gaactaatat agaatttagc tgggttttca gcatcttgag aagaaaatac aacatgtcgt
    27361 gtttcttttc cgatatagta tccgtcttgt tttttcattt cttctttttg cccatcgatc
    27421 tcgatgaatc ctggtccacc gatattgatc acacctagtt cacgacgctc taagaagtaa
    27481 tcaacaccta gttctttgtc taatgtgatt tctaattctt tttcagtagg tgtcactcca
    27541 ccaaagatca ttcgatcgtt gtgcgtataa gtcaaactga ttttccctgg ttcaaatacc
    27601 ttttctacta aaaattcttt tctcaattgt tccgttgaat aatgacggat atcttctgga
    27661 ctgtgtgtat aacgtgtttc catttcttgc attttcttct actccttcta tcgaatgatt
    27721 tttccagagc cgcatgctaa aaattgtgcg acttcttctt gactaaattg gttgcagtcc
    27781 ccatggatcg tatgcttcaa ggcagaagct gccgtagcaa aatcaatgat ttcttgcgga
    27841 gacatattgg ataaaatccc atggagcacg ccacctgcaa aagcatctcc gccacctact
    27901 cgatccacga ttggggtgat tctatggact tgggattcca catattcatg gttcatccac
    27961 aatgtcccct tcaattcatt ttcacttgct gaatgaacgg tacgcttggt cgaataaaac
    28021 acctgaatat tcgggaactt ttcctgcatt ttttgataat aataaattag aggttgttct
    28081 tctgcttctg gcgctttctc gatacctaac aaataaagag catccatttt tccagctgag
    28141 cagatatcta ctaaaggtaa aagctgatgg attacttcgc tggcttcttg ctgtgtccat
    28201 aattttccac gatagttgat atcgaagctg atcaggcagc cagctttttt tgcagcttct
    28261 atcacctgtt tcgtcatttt ttgccatttt ttagacaaag ctggggtgat tcctgaaaca
    28321 tgaaatatat ctattccttg gaataactcc tctagcgacc attccaaatg atccatttca
    28381 gcaaaacttg atccggcacg atcatagatc actgaggcgg cgcgttcgcc actaccagtt
    28441 tccatataat aagtacctag tcttgatcca ccagataata cctgtttcgt atctactcca
    28501 taacattgca agtgcttttt gactgctttt cccaatgcgt tatcaggtac ttttgtagca
    28561 aaaatcgctt catgtccgta attagctaaa gaaattgcta cgtttgcttc ccctccgcca
    28621 taatgggcag cgaatcgttc acttgaatcg atacgggttc cctgctctgt ggacaatcgc
    28681 aacatgattt ctccgagtgt cactactttt cccataagca gctacctctt gatttctttc
    28741 atttttgctg catactgttg cgcgacttcc gttactttgt caaaatctcc tgtagctgcg
    28801 ggtgctaaaa ggttcccacc tactccgact gttacgacac cagcatcgaa ccactctttc
    28861 atattgtcca gactcacacc gccagttggc atgatattga cttgaggcat cggcgccttg
    28921 aaggcagaaa tgatacttgg tccataagcg cttccaggaa atagtttgac gatatctacc
    28981 ccgcttttca gcgctgtttt gatctcggta atagtcatac atcctggtaa gtaaggaatt
    29041 tgatacagat tgcacatctc tgccgtttct tgatcaaagg aagggctaac aatatactct
    29101 gcgccagcta agatagccaa gcgagctgtg acagcgtcta atactgtgcc tgcgccaatc
    29161 actatttctg gttggtcttt gtagaatgac aaaagttctt tgattacttg atctgcttga
    29221 gggactgtaa aagtcaactc aataccagtt agaccacctt tgatgattgc gtggcaagca
    29281 ttcaaagctt cttctttgct ttttccgcgt accaccgcaa tcactccggc gtttttcaat
    29341 cgagataaaa tatcaatttt tttcatcttc tccacctatt tcctatttag aaattttatt
    29401 ttataacttg gaaatctata cccatattat acggcagaaa agaaaaattg caaacgcttt
    29461 tttcattatt tttgaattct ttgctcgatt tcgctttttg tttgcttgag ggcactgatt
    29521 atttgttctt ttgtctcatc tgttaaacga tatttcggca tacttacact aaatgcacca
    29581 atgatttgtt cgtttttcac gattgaggct cctatacaaa aaatatcacg ttcgatttct
    29641 tcatcgtcaa atgcgatgtg ggtttctttt accgttttga tttccttttt caaccgtaat
    29701 gcattggtga tcgtatattc tgtgtaaggg atcaacggat gcttttcaag ataatcagtg
    29761 tactgctctt cactgaattc tgctaatacg gctttgccca tcgcagaatt gtataatggt
    29821 cgggtgattc cgatttttga agacatgcgg atcgtttgat gttttggatc tagtttgttc
    29881 acgtacaaga tctcattgtt gtccaatacc cctaagtgga tagtctcatc gatcttttgc
    29941 tgcagctgtt ctaaaaacgg taaagtcaac tctaccaaat cgatttgttc aatgttttta
    30001 ttcgcgtagc gaataagctt tgcacctaaa cgatagtttt tctctgaatt tttatttacg
    30061 tagccgatca tcaacaaagt atctagtatt ttcaagactg tagaagaggt cattcccaca
    30121 ccctttgcga tcgtctgcaa agagacatct tggttttccg ctaaatagtc tagtatttta
    30181 gaggctttga tcaatacagt tccataaggc tttttatttt ccatataata ttctcctatt
    30241 tctttataag aaaaattata gcggaaatac catcaattta aaagagatat ccagatataa
    30301 actaccattt ctctttacag gcaatttaat gttgaagatc cgcttcgttt ttacatcaaa
    30361 ctttgatata aagcaatgat ttcttctact gtcgtttctc ttggatttcc tggtgtgcat
    30421 acatctcgca aagcatcttc tgcaattgct ggaatatctg cttcttttac acctaattca
    30481 gaaatggttt ctgggattcc aaccgctttg ctcaaccgat cgatctcacc acaagccgct
    30541 tctcttacat cttctaaaga catttcagct gctcctttaa tccctagaac aagagcgatc
    30601 tcacggtact tttctccagt atattctttg ttatatttca tgacagttgg caaaagtgct
    30661 gcacacgcta cgccatgagg aatgttgtac catgcactta acgggtgagc catcccgtgt
    30721 accagtccta aaccgacatt tgagaagccc attcccgcaa tatactgtcc taatgccatt
    30781 ttttctcgcc cattttgatc gccatctaca gaagcttcca aagaatggcc gatgatttcg
    30841 attgctttta aatgcagcat atcagtcatt tcccaagcgc cttttgtgat aaacccttca
    30901 atagcatgcg tcatagcatc catacctgtt gatgcagcca attttttcgg cattcccatc
    30961 atcatatcgc tgtcgatgaa cgcgacgatt gggatatcat gaggatcaac acaaacaaat
    31021 tttcggtgat ttacttcatc tgtgatgacg tagttgatcg ttacttctgc tgctgttcca
    31081 gaagtcgttg gtatagctaa tataggtaaa catggatttt tcgtatcagc aacaccttct
    31141 aaactaatga cgtctgaaaa ctctggattc gtcaaaataa tcccaatggc ttttgccgta
    31201 tcgattggcg aacctccacc gattgcgatc aaacagtctg cctctgcctc tttaacaaaa
    31261 gccactccat cttttacatt ttggatcgtt ggattaggta cgatcccgtc gtataaagca
    31321 taagcgattg ctgctttatc tagaagttct gttacttttg ttgctatacc gtgctcgatc
    31381 aatcctttgt cagtaatgac agctgctttg ccgaatcctc gctgctgaaa ttcagggacg
    31441 atcgactcaa tcgctccttt tccaaaatac gatgtttcat ttaaaatcat tcgattactc
    31501 attttctatt cctcctaatt tgtattattt ttcacaataa aaacgaaaga accccaagca
    31561 accggttgcc cagttgcttg ttgctacagt tcaaagactg gcatcaattc ccaactgact
    31621 ggagaggcat ctggatttgt ctccatgata tcttccatgt acttccacca tttttgattg
    31681 atagatgtat ttgccatttt ttcccataac ttttcatctt cgatcactaa gtaaccaaat
    31741 aacaaacttg tactcggatc aagatagatc gaatagtcga tgaccccgtg ttcacgaagt
    31801 gctgtacgca tttctggcca aagttcatca tgtctttttt tgtattcttc ttgtttttct
    31861 gggtaaactt tcatgcaaaa agcttttttc accatgtcag acacctgctt tcgtatgaac
    31921 ttcctccaaa taacctgctt ttggagtgac accaaacgca tctgctaaat gccacaggtt
    31981 ttcgtccgtg attgtttgca agattggtcc ttgcgctttg acgtacgtat agacttctgc
    32041 tgccttttct gctgtctcga tcaaaccaaa gacttcatcc atgtcttttc ctgacccgta
    32101 gattccatgt tgtggccata aaaccaatcg agtttctttc attttttcag ctgttgcttc
    32161 cccaatctca tttgttcccg gaaccatcca cggaatgatg gaaattcctt ctggaaaaac
    32221 aactaagcat tccgtacaca tttgccatag cgtacgacta aaggaacgtt cgtctaattc
    32281 atgcgtaaaa ctcatcgcta atagatgact agcatggcaa tgcataacga tccgattatc
    32341 tgggtctact tctaaacggg cgatatgact catgaaatga cttggcaact cgcttgtagg
    32401 aaggcattcg ttttccagcc cccacaaaag ttccagtgtt ttgccatctt cagcaactcg
    32461 aacgattcca agattctcgg caggttcgct cgctacattt ttaaaatatc ttcctgaacc
    32521 agttacaata aaatagcatc ctgctaaacg gctggcatca aaaatcatcg agatgcggcg
    32581 gatcactttt tcaggatcca agaacggtgt gatctcttct tcttttagta aataagaaat
    32641 attcccgcca tttctttcat cccatcccaa gcgataaaga ttcgacgtcg tttcgatcat
    32701 ttctttcaca taaggcgctt gtaaaatatc tatttttttc aatatagcca cactcctatt
    32761 ttcttaattc caacacgtct ttttgatatt cctggacttc ttccagccaa tccaaaccaa
    32821 ctggaacttg atttttgtaa cagaaataat tccatacatc ggcatacggg aaatctttca
    32881 attcttccgt ataagccatt cttgaagtaa agtcaaaagc cagctccatt tctttcaatc
    32941 gctcaactgg ttccagcata gctttcagca gtgctttttg tgtatttctc gtaccgatga
    33001 cccaagcagc gatacggttg atcgttgcat caaagaaatc cagaccgata tttgtttttt
    33061 ccaacaattc gttacggact aattctttag cgatttcctg caattcatca tccatcaaaa
    33121 ccacatggtc tgaatcccaa cgcactggac gggaaacatg caacatgatt cctttgctga
    33181 ataaagctaa tgaagataat ttattggaaa tcacttctgt tggatgaaaa tgtccagcat
    33241 ctagacaaac aagtttgtca cgagtcaacc cgtaacccat ataaaattca tgggaaccga
    33301 ctgtataagc ttcagcgcct aaaccaaata gtttgctttc tactgcatct tgtacatacc
    33361 gttcatctaa ctcttctgta aaaatttcat cgagtgattc cattaaacgt tcccgtgggg
    33421 taaaacgatc aatcggatta tctttcatcc catcaggtac ccaaaagtta ttgatacttg
    33481 tttgcccagt ttctttccca aagtactccg cgattttgcg gcttcttttc ccgtgttcga
    33541 tccagtattc acgaacttct ttatctggat gagccaatgt aaagccgtct ttcatcattg
    33601 gatgcgaaaa gaaagtcgga ttgaagtcaa gaccgatttc ttcttttttt gcccactcta
    33661 cccatttttc aaaatgtttc ggctcaattt catttagatc gacagattct tctgtatcca
    33721 aatagatagc atgaaggttc aatttgtgtt tgcccggtat caacgaataa gccttctcca
    33781 gatcttgtct aagctcttca ggtgttcgtg cggctcctgg aaaattccct gtcgccatga
    33841 ttccaccggt caattcgcca tccggtgtca agaaaccttt gacatcatct ccttgccagc
    33901 aatgcataga aattttgatg tcctgtatct tttctagtac agcatctgta tctacaccga
    33961 tagttgcata tttttctttt gcttgttcgt aatttctttc aatcgtgttc atacgattac
    34021 tcctttgatt ttattcacgt accattccat cgtgttttgc ttattttcat ctgggtaaaa
    34081 agtttcacat ggaaacgact gtttgattac atttcttgct tgttctatat tcgaaaactg
    34141 acctgtactg agcatttgca tggcaacatt cccgattgct gtggcttcac taggtcctgc
    34201 ttctacaatg attccggcat aatctgctgt caattgattc agtagacgat tattcgatcc
    34261 gccccctacg atgtgcagct tttccaattt cgttccagtt aagtgttcta ctttgatcag
    34321 ctcttgtgcg taacaaagag caagattatc atacacacaa cgagcgatag ctcccgatga
    34381 gacagggact ggctgctctg tttcaaaaca gtattgctga atctcttcta tcatatttgg
    34441 gggattcaaa aatcgagggt cattgatttc catcacttgc tgcagcggtt tttcttttgc
    34501 agcaagctct gccaattccg cataactgta ctcgtactct tgcattcggg caacttcctg
    34561 gatcaaccac atccccatga tgtttttcaa aaaacgcacc gtgttatggg caccccattc
    34621 attcgtataa ttttcagaaa aagcattgcg agaaacattg gcgatctttg tttcaatccc
    34681 taaaagcgac catgtcccac tgctgatata accccaacgt tctccttgac ctggcgttcc
    34741 taatacagct gacgccgtat catgacttgc tactgtgata aaggtcacat ctggcaaatc
    34801 atattttgaa aattgttctt tttgcagatc tcctagtatc gttcccggct ctgtcaatgg
    34861 tggaaataat tccttcgaga caccgatttt ttcaagaagt tctatttccc attcttttgt
    34921 ccctgcattc agcagctgca tagtagatgc attagttttc tccgtcacca tgttaccagt
    34981 aaagacatac cctaaataat caggtatcag taacaattta tgtgcttttg ccaaattttc
    35041 tttttcttca gcaaacaatt ggaacaaggt attgaaaggc tggatctgaa tccctgtctg
    35101 ctcatacaac ttatctagag aatagatttc agaaaactgt tctactgctc cattggtacg
    35161 cttgtcccga taagcaatcg gatcatttat ccgatcccct tgttcatcaa tcagacagta
    35221 atcgactgcc catgtatcga tccccgcaaa aaggcgatca atccctgtct gctttaattt
    35281 ttcaaatcca atcaaaatct ctttgataag ataatcgatc ttccagcgat cgtgaccgtc
    35341 tttgttccga aagccgtttt tgaaccggtg tacttcttcc aagatcaaag aaccttttgc
    35401 atccctcgta cttttcatta gtcgtccgct tgatgcaccg atatcgatgg caacaaatgt
    35461 tggattcatt tctccacctc ttttacttta ttgttgatta gcactttatt aataaagcgt
    35521 tatcatttat tgacaatgct atcataatac agaatcttat attttgcttc acactatttt
    35581 ggtatataat tgcattatct tgacaagttg ggagtgttta catggattac tgggaaaaag
    35641 ccaaccactc atggtcggaa gactcaaaaa gagtgatctt gacgcctaca caaagaagca
    35701 ggaatttatt tttttatatc caagaaatcg gttacttcaa agcaccaatc ccttactata
    35761 ctgaacgagc acatttacct tcttacttga taaagtttac tttgtctggc aaaggttcgc
    35821 ttacatataa tgataatcag caacagctaa aagctggaga cttatttttt attgactgcc
    35881 aagaatatca gcgttatcaa acgatcagcg aagaaccatg ggaaatggat tggatccatt
    35941 tgaatggtca gaacgtccaa tcattttatc aggagttcac aaaagacggc cataacacct
    36001 tccattcaac aggagatcca tcagaaaaca agatccacca tttgatcaca gaattgatcc
    36061 acatgcaaca aggcacgaat gcccgtaccg atttccagtc ctctgtagct atccacgagt
    36121 tgctgaatga actgattttg caaaagtatc agctagattt cgaagaagag gatattcccg
    36181 cctacgttta caaactgaag caatacttag acgaacattt caaagaaacg attacacttg
    36241 atcaattaga acaacaattc ttattgaata aatatcagct gagcaaagat ttttccaaat
    36301 ttatcggttc tccaccaatc gattatttga tcagcaaaaa actttcttac gctaaagacc
    36361 ttcttcggta tacagaattt acggttcgaa tgatttccga agaaataggg attgagaatt
    36421 ttgcttactt tagccgcctg ttcaaagcaa aaacaggact atcgccgaga gactttcgta
    36481 aattcagctg attcatataa tatgtacgtc attttttcaa aatattttga aatatataga
    36541 taaaaataaa ataaccgcag tttctgcttc tatgtaaaca acagcagcta ctgcggttat
    36601 tttttagtga tcattctcat tgttcatctt caaaatgttc ataatgaacc aactgtttct
    36661 gtgtatcacg tttcaaaaat tctttaggcg ttatgccgaa ctctctcttg aatgctttca
    36721 gaaaatgcgg ataagaagaa aaaccacttt tactatatac agcactagag ctgcggtatt
    36781 ctcgcaacaa gtgcttggca tagatcagct ttttcttcat tacatactga tgaaacgtaa
    36841 atccagtgta ctttttaaat tctcgtgtga tatggtattt gctgacaaaa aactgctttt
    36901 ccatttcctc taaagaaagc ggatgagata aattttttga gatatattgt aacatttgtt
    36961 ccacacgctc attaggaact acagcattct gttcttgcgc actctcttct ttttgtacaa
    37021 cttgattgag aaaaatcaag tattctacca gagcttgttc atattttatg tcttcaccgt
    37081 aaattttttt atcaggagga tggtcgataa atgataattt ttgcttcaat acttcctgtt
    37141 ctatttgcaa tacacgactc ctcttctctc ccattggttg aaaacaagcc gatagattag
    37201 accatggcgt ggaacgactt tctagaaaag aaggggtgac aaagatatag acacgctcga
    37261 atacatctga agattggcgg acgatacgat gcaaatcatt ggaatggatg agcagcacac
    37321 ttcctgcttt gatcgtaaat tgtcgtccgt tcaaataaaa agtcgcttct ccctttaatg
    37381 tagcatggat ctcatagaaa tcatgatgat gatagatcgt tttattttgg ctgacgacat
    37441 ctttgacatg atagatttca tactgatcag aaaccatcgt attgacttgt tcaccgctga
    37501 ttttcttttg gagcatgtgc cgcacctcct tttgttcact tatgcacaat tttattatac
    37561 cacgtttttt gtaatcaaaa agagctgctt ttatatactc ttataaaaaa gaagagacgt
    37621 ggcatcttct agccccgccc cctccattct aaatattagt taaccaacgt cttctttaca
    37681 ttattcaaga agacttcttc cataacgaat cccatcatct tttgtttctt caagaaccac
    37741 atacaggtac ggacgattct tcatgatgat ttctttgatt tttgtgtact cgatcacacc
    37801 gtctcccaaa tttaccggca cgattttttc ctgttcgaca cgaaaatctt tcaaatgaac
    37861 cgcacatatc ttttcaccaa aacgttcaaa agcttcttct accaattgga cttgttcttg
    37921 ataatcacta cttgtgatca aattggtcgg atctaaaatg attcctagat agtccgaatc
    37981 tactgcttgg atcagctgct ctacccttgc taaagaatac accggatgat tcaaacctgg
    38041 ttctatcccc accatcattt ggtatttttc tccagctttt accaagcgac ggataacgga
    38101 cacagcttca gcaaatgctt catctgtgaa attttcttcc gtatattgga tttccggcaa
    38161 aacacatcca gtttccgaag caaccatcga cgcaccaaag gaagaagcat agcgaagata
    38221 ctgctcgaac tttgtcagca actcttgtcg tatcgttaaa tctggatgaa tcatattgat
    38281 atagcaactt aaaattccta cggtcacttc ttgcttttct aaaactcgtt tgacataatt
    38341 tcccatccca gaattgatct catttgcgcc agaaggtatt tcaggaaacg aaagacctaa
    38401 ggcgagctgc agtgtatgaa tcccttgttc ttttgttttt tctgcaagat cttctatcgt
    38461 ttggacttcg gaaagatcat gtcctctaat tgtaatgttc aacataacta ttctcctaac
    38521 tcagcatatt gggtagccac atgacgattt gcgggaagta agtaatcaaa aacaagacga
    38581 ctaagataac cccataaaaa ggaagaagcg gtttcagcat tgcctctgct tttacgcctc
    38641 ctacacttgc accgacatat agtcctgtac ctactggcgg cgtgatggag cctacacaca
    38701 gattcatgat tgaaaacaac ccgtaatgta caggatcaac ccctacactt gtgacgattg
    38761 gtaaaaatat tggtgtaaaa atcaagatag caggcgccac atccataaac atacccacca
    38821 gtaataacac aagattgact aataacagaa taataaattt attatccgtt acgcccataa
    38881 tcaacgaact aattgcctga ggaataccgg taaacgccat ggcgaaagac atcatggaag
    38941 aagtagccaa taagaacatg atcgtccctg acattgctac tgattgaaac aacatattag
    39001 gtatatcttt tagttttacc gttttatagt aaaacatagc aatcaacagg gagtaaatga
    39061 cagctatcgc tgaagcttct actgctgtaa atatccccgt caagatacca ccgataatga
    39121 tgacaatcaa tagaaggcta ggaatcgcct cttttattat ttttccagca tgagccatct
    39181 gtcctttttc gacaacaggg tatttattct ttttagcaac gatgaatgct actagcatga
    39241 ttcctagcgc ccacaaagcg cctacgagat aacctcccat aaacaaagca gagatcgaag
    39301 caccaccagc aacaagtgaa tacatgataa aagcagtact tggaggaatg accatacctg
    39361 tcggagctga cgcgatattg accgctgtcg caaacttccg gtcatatcct tcttccactt
    39421 gctgagggat caacactccg ccaatcgcgg tagaagcggc aatggccgag ctagagatgg
    39481 aaccaaataa agcattccct aacacattcg tatgagccaa agcccccggc acgcgcccga
    39541 cgaacaacat cgcaaaatcc actagtttct tagctatccc tccattattc atgataatgc
    39601 ccgagagaac gaaaaacgga atagcgatca gcgaaaaact attgatactt cccaccattt
    39661 tttgagctga actaaaagca gaaacatcaa acggtataac taacaataaa gtcatcatcg
    39721 atgagaatgc aatacttata gcaatcggca tccctaatac aagcaacaca acaaatacaa
    39781 aaaataacac taatccggct tctattgcca ttttattcct ccatttttat attttttctt
    39841 gaattgctgg cttgttgctt aatgtatcga ttagattgat caggctatag ataatgatga
    39901 gtacccccga tacaggtact gacagataaa catatcccat aggaatactt aaagaaggcg
    39961 agatctgccc cattgtcaat gtgacagcat tccatccgcc atacaccatg atcatcgcag
    40021 caaataccag aaataaactt tgaacaacaa cttctaaaat tttacttttt ttgccagtta
    40081 atttttcact taaaaccgtt acggctaaat gtgctttttg tcccacaaca tacgcggaag
    40141 acaacatggc aaaccagaca agaccgaatc gaacaagctc ttccgtgacc gtatttggat
    40201 ttcctaaaac cgttctggaa aatacttgcc ataaaacaac agcgaccatc ccaacaagca
    40261 aaacagctcc caggatctct aataccttgt ttaaaatcat tttcatcaca gctacgctcc
    40321 ttgctcttgg atcttttgat aaaggtcttt gtaggtagga tcgtttttga atatatcatg
    40381 aagtggggca actgcctctc gaaacggtgt attatctaca tcattgaatt ggactccatg
    40441 ttcttcttgt gctaaagctt tttcttcttc aattgctttt gcaaaagctt caatttcaaa
    40501 aaccgttgat tctttcgctg cttcttcaat ggctttttcc tgctcatcag ttagctgttc
    40561 tttgatggca tcattcatga tgatgatatc tggcacacgt gtatgctcat cataggaata
    40621 gtatttagcc acaccaccat gtccagctgt atacaagaca aattcattgt tttctgatcc
    40681 gttgatcaaa ttggattgaa gagacgtgta cacttcatca cttcccatag gtacgggcga
    40741 accgcctaaa agctctacca ttttgatagc ggtttcgctt tgcatcacac ggatcttctt
    40801 tcccttcaaa tcttccggcg tatggatcgg accgtctacc atataaaagc tgcgctgacc
    40861 agaatcataa taggtcaatc cggtaaatcc taaatttttg gtcgattggt agattggctc
    40921 cattatttct tcattatcca tcaccttaaa gaaatgttct tcatcattga atagataagg
    40981 aactgaaaaa atcgaataat ctttagagaa actttccaac gctgaggcac ttacttttgt
    41041 gaaatctact gctcctgact gtgtcaattc gatcagttca cgttctccac ccagttgtcc
    41101 atctgggaaa tactggatct gtacggaacc atctgttttt tcttttacta aacgcccaaa
    41161 ttcattcatt gcatcaatta ctggctggct gttgcttgca taagcaaagc gcaaggtaac
    41221 tatctcattt acatctgcat ctgcgacagt tgccacacta gaattatttc cgcagccgct
    41281 tagacctagc agacctaggc taagcgaact tgctgcaaac agcagtgatg tttttttcat
    41341 atgggtttcc tttcttttaa caataagaga cgagcaattc tgtcaaacaa agcaccgtta
    41401 acgactgtcc ataaggcatt gctgtgattc cgatattccg ataaaaatct aaactgtccc
    41461 ccattcctgt cccgattgaa acgttttgaa cttctccttt ttcatcgatt tgttcaagca
    41521 atccttggat agcacgataa gcgacttctt tatattcttg tggtaaataa cgtttatgga
    41581 ccgctttcaa aataccgtaa gcaaatcctg cagttgcaga agattctacg taagaagaag
    41641 gatcatccaa caacgtatgc cataaaccgc tttcagactg ataagttttt aatgcacgca
    41701 cttgtgcttc aagtgtttcg atcaataatt tgcgcaatgc atcttctttc gataattcta
    41761 agatttcaat gatctctgga atagcaatcg tcaaccagca gtttccacga gcccaaaatg
    41821 cttcggcata gttatgattt ccttcaaacg tccagccgtg ataccacaat cctgttcgtt
    41881 tatccatcaa gtatttgata tggatcataa actggtaacg agcttcttct aaatattctg
    41941 ggcgattcaa cagtttcccg atttttgcca aaggaagcac tgtcatcatc aatgtatcgt
    42001 cccacaactg attcttattt tccggtccat atgtggcatg ctgtaacccg tcttcatttg
    42061 tccgaggcat gtcattcatg acccattctg cccattgttc taaataaggc aaatagcgtg
    42121 aatctttcgt gtcttcatac aagtaagcca tagtcaatag cggtgccatt gtattgacgt
    42181 ttttgggcgg agcgccttct tccatacgat cctcaaacca ttcattgacc acttgataag
    42241 cgcgaggatt ttttgtcatc tggtaatttt tataaatacc atatagaccg acaccttgtg
    42301 gccaattcca cactgtccag cttttgtcat ctactttcag tccatcaaag tccaacaaaa
    42361 attcaccatt atcgtctttg atcgttgtca ggttttcaac gagcaggtca attttttctt
    42421 tcactaattc tttaggtaca aaaatctctt ctttaataat catctttacg ccaccttttt
    42481 aaacgctttc attttatatc cttaatatag cagtaatacc gtccactata aatatcgctt
    42541 tttgaacaaa aactgtctaa aattgaccta aatgttaaat aacaaaagta actttcagtt
    42601 acttggaaat taaaaaaaga acttggagaa acaacgtttt ccaagttctt tttcatctat
    42661 tttcttatct tttttctacg ctttcttttg cttatccgtt gtaagcatca atgatgatct
    42721 gtttcaattc agagatcaat ggttctttcg gattagctgt cgtacattgg tcttcgtaag
    42781 ctaattcagc catacggtct acggttgtat ctagtgtttc ttgtgtgact ccttgagctt
    42841 ttagactcat gttgatgccg acagattttc ctaattcata aacagccgtt gctaaagctt
    42901 cgactaattc ctctgttgtt tcgcctttca gacctaagaa ttttgcgata tctgcgtaat
    42961 ctgtatctgc acggaaatag tcatatttag ggaacatcgc atgtttttgt ggatcttttg
    43021 cattatagcg gataatatgc ggtaaaagaa tcgcatttgt ccgtccatga gggatcccgt
    43081 attccccgcc gattttatgt gcgactgagt gacaaatacc taggaatgcg ttagcaaacg
    43141 ccataccagc cattgtagaa gcgttgtgca ttttttcacg tgattccata tcaggtgttt
    43201 ttactgattt ttctaaatag tcaaagacta acttgattgc ttgtaggctc aatccacgtg
    43261 tataatctga agccatgact gaaacatagg attcgattgc atgtgttaac acatccatcc
    43321 cggtatctgc tgtgacagat gctggaactg acatgacgaa ttgaggatcg acgattgcga
    43381 catctggcgt caatgcataa tctgctaacg gatatttcac atgtgtttca ctgtcagtga
    43441 tgaccgcaaa tggcgttact tcagaaccag tccctgatgt tgtcgggata catacgaatt
    43501 gtgttttttc tggtttgtcg atcttatatg tccgtttacg gatatccaag aatttttgtt
    43561 ttgcaccaaa gaaagatgtg tctgggtgtt catagaacat ccacattccc ttagctgcgt
    43621 ccattgcaga accgcctcca agagcgatta ctgtatctgg cttgaagtct gccatcagtt
    43681 tcgttcctgc gtatacggta tttgtcgatg ggttcggttc aacatctgag aagacttcga
    43741 tcttcacatc atttttacgt cgtgccaata cttcacgaac tgtatcgcag taaccgaatt
    43801 ggaccatacc agggtcacaa acgatcatga cacgttcaac gttttccatt ttctctaagt
    43861 acaatagaga gttcttttca aagaagattt ttggcggtaa tttgaaccat tgcatgttat
    43921 ttctccgttt cgctacagtt ttgacattga ttaagtttac agcagagacg ttacgagaaa
    43981 cagagttctt accataagaa ccgcatccca aagtcaatga cggaatcatt tcgttataga
    44041 tatcaccgat gccgccttct gcagatggtg tattcactaa aatacgacaa gctttcattc
    44101 gaagaccgaa tttgacatgc aactcttcgt tttctgaatg gataactgct gtatgtccta
    44161 atcctccaag attcaacata ccttcacaca aatcaaacgc atggtccgtg ttgttagctt
    44221 tgatcatggc aagaactgga gaaagttttt ctcttgatag cggataatct gggcctacgc
    44281 cttccaattc agcaatcagc attttcgttc cttttggtac actgattcct gccaattttg
    44341 cgatttccat cgcagaatgt ccaacgattg atggattgac cgcatatttt ccttcgttca
    44401 tcactgcatc ttctaatttt tgcaattcat ctggttttac gatataaact tggtgtgctt
    44461 ggaattctgc ttttactgct gcatagattt ctttatccac gatcacagct tgttcagaag
    44521 cacaaatcat tccattatca aatgttttcg aaacgatcaa atcgttcact gcacgtttaa
    44581 tttttgctgt tttttcaata taagctggta cgttacctgg acctacacct aaagctggtt
    44641 ttcctgtaga gtaagcagac ttgaccattc cagctccacc tgtcgctaag acgatcgcga
    44701 tacctggatg gttcattagc atagaagtcg cttcgatcga aggatgttcg atccattgga
    44761 tacaattttc aggagctccg gcagcgatag cagcatcacg taccactcgt gctgcttctg
    44821 cagaagattt ttgggcactt ggatggaaag caaaaacgat tggatttctt gttttcaacg
    44881 cgatcattga tttgaaaatc gttgtggaag tcggattcgt tgttggagtt actccgcaaa
    44941 ccacaccgac tggttcagca atttcaatca accctttttg gacatcttca ttgatcacac
    45001 caactgtttt atcatgtttg atattatgcc aaatatattc ggacgcatac atatttttga
    45061 ttgctttatc ttcgtaaaca ccgcggcctg tttcctctac agccatcttc gctaatggca
    45121 tatgttgatc caaagctgcc attgccattt catgcacgat atggtctact ttttcttgat
    45181 caaagctttc catttctttc aatgctgcat ttgctttttc tgccaaatca tcaatcattg
    45241 cttgtacgtc aattgtcttc gttttttctt tttctattac tttagccatc attatcctcc
    45301 taatgggtca tggttatttg ttaaataatt cacatgtata tcatacaata tttatttttc
    45361 tatgtaaaca cttttttgtg atttttttca cagttatttt taaatataaa attgctttat
    45421 ttacaggtgt ttgtatagaa aaatatataa agttatttat tattatttat atgacacacc
    45481 cgattattca atattgagca atatatccca tgctcttatc tctttctttt ttgaataaaa
    45541 aatgaggtag gacatctttc tgccctacct cgtctttaaa taagctttga tacttatttt
    45601 tcttccttct cttctgtctc ttccagcgtt tctgttgttt cagtttttac ttcaactgtc
    45661 tcagctggtt cttctgttgt ttcaacagta gtcgttgcat catttgttac gacagtccct
    45721 ggtttgaccg ttttgattgc tgcacgatcg tactcaagga agatcccttc gcagtctaaa
    45781 acaactgtcc gtttgtcatt gtcgatttct gaaacaactc catgtaatcc gccgatcgtt
    45841 acgactgaat cacctggttt catgctatct aacaagtttt gacgttcttg ctgttgtttc
    45901 ttttgcgaac gtgacataaa aaaccacatt gctaatagcg cgataaacat cacaagcatt
    45961 ggtaatgttc ccattttttt cacctcacta attctgtatc tacaaaaaac actttagcag
    46021 aaaaatgctg ataacactag ttttttactt aatttttaaa agattttcct ttagaagctt
    46081 tttgcgtttt ctttattgaa cccgtattct tcaaaaaagg cttctctaaa ttcaagcaaa
    46141 ttatcatcca tgattgcttg acgtacttgt ttcattagat tcaacaagaa gtataaattg
    46201 tggtaagaag tcaaacgaat cccaaatgtt tcatcacatt tgatcaaatg gcggatatag
    46261 gcacgtgtat agtttttaca tgtataacaa tcacatttct catcaattgg acggaaatca
    46321 tgggcatatt tcgcattttt cactactaga cggccttgag aagtcataca tgtgccattt
    46381 cgagcgattc gagtaggcaa gacacagtca aacatgtcta ctccacggat aacaccatcg
    46441 atcaatgaat cagcagcacc gacacccatc aagtaacgtg gtttgttttc ggggatcaac
    46501 ggtgtagtga attctaatac acgattcatt tctgatttag gttccccgac agataacccg
    46561 ccgattgagt aaccagggaa atccatcccg actagatctt tcgcgctttg acgacgaaga
    46621 tcttcaaacc ctgctccttg gatgattcca aataatcctt gacggtctgg attagcatga
    46681 gctttcaatc ctcgttctgc ccatcgagac gtacgttcta ctgatttttt cacgtagtcg
    46741 taactttcat cgaatggtgg acattcgtca aaactcatca tgatatcaga gcccaattta
    46801 ttttggatgt tgattgcttt ttctggtgat aaaaacatac gcgaaccgtt caagtgattt
    46861 ttgaaatgca cgccttcttc ttcgatattc cgcatatcac ttaatgaaaa aacttgaaag
    46921 ccgcctgaat ctgttaaaat cggttgatcc cagttcataa atttatgcag tccgccagct
    46981 tcttctacta agtcttcacc agggcgtagc cataaatgat atgtattact taaaataacg
    47041 ccagcaccca tttctttcaa ctcttctggg gacattgttt ttactgtagc taatgttcct
    47101 actggcatga acattggtgt ggggaaagtt ccgtgtggcg tgatcaattc ccctaaacgc
    47161 gcgccagtat gtttttcttt cttgattaaa cgatagcgaa tggcaggttg cgtcattcat
    47221 ttccctactt tctatataaa atatcttgac aactcccacc atcataatcg tttcaagtgc
    47281 tttttgcaag ttccctcggc ttccttctac tttatttaat attctttttt ttatcctttt
    47341 tttatgaata acaaaaaaaa gaaaaacatt atgacagaaa tattttttga tacatggtat
    47401 attggtgtta ttaaagaaaa ggagggaaaa atcatgcaat cttctgcaga attaaaacgc
    47461 caagcaaaag agagtctaaa aggacgttgg ggacaagctg ttttactaaa tctaatccct
    47521 acattgattg gtattgctac tattcttctt attatgattc ctattatctt gtttgccacc
    47581 accatatatg atggatcagc gagcgtctca gaagcgacaa cttcaggagg cggtagttca
    47641 ggaggcggtg gcggaattat ttccagcatc attagcgctt tgttcatgag cggtatttct
    47701 tggacttatc ttgatatttt acgtggacaa aaagatcaga tcgaaccatt caaagatgca
    47761 tttcgtggat tcaaaggttt gtttatcggc ggagtgatca gcttagctgt gctgatctcg
    47821 atctttacaa cattatgggg tttgctatta gttattcctg gaatcattaa aagctattct
    47881 tattctcaaa gctattttat ttactacgat atcgtccaag aaacaggaca aacacctaaa
    47941 gtactggata cgatcactgc aagtcgaaaa ttgatggatg gttataaagg acaacttttc
    48001 tggttagatc tttcatttat cggctggcat atcttagcat tagcaactgc tggtattggt
    48061 tatctatggc tcagcccata catttctgca acaaaagcag cattttatga acaattgcca
    48121 aaagatattt aaaagagagt gtgacttgtg accgaggggg tggcaggaga acaccgttta
    48181 tcgagaggca gtaaaagaaa cattcagccc attagcaaaa aggaggtgtg acaacattca
    48241 tgtcacacct cctttttgta tagttccagt aaaaagaaaa tcaacttaga aacagttaat
    48301 cattcctctt cttctgcttc cggtaatcga tggaacttga tcagttcatt caccgcgatt
    48361 ggtacgatag ataaaatccc taccaagaac cagtgattca aactgatgtg tactaggccg
    48421 aatagttctt gcgtaaatgg cagcaaagca acagccgttg taatagctaa tgccagtaac
    48481 gccatttcta ccaaagcttt attggtcgct aaattgactt tgaagatcga ttggctgctg
    48541 cgtacattga acacatgcag aatcgatgaa taagctaaga tcaagaatgc aacagtttga
    48601 ccgacatgct gacttgcgtc tacccatggg ctgatagtat cgacataact acctaaataa
    48661 taaccaaaca aggttacgat ggtgaagacc cctgcattta tggcaatttt ttgcaataaa
    48721 cctcgagcaa agatcccttc atttttcgga atgggtggct gatccatgat gccgtgttcc
    48781 gctggctctc ttcctaaagc aaatcctgga agtccatcag caactacatt gacaaacagt
    48841 agttgcacag ctgtaaatgg tgctccccat cctaacagca tcgcaatcaa aacaataaag
    48901 atctccgaaa tattacagct tagcaaaaag ttgatagctt tacgaatatt ctgataaacc
    48961 gaacgtcctt gactgacagc gtctacgatc gtcgcaaagt tgtcatccgt caaaatcata
    49021 tctgcggcac tttgcgccac atctgttcct gtgatcccca tggcacaccc aacgttactt
    49081 gctttcaacg ctggtgcatc attcacacca tccccagtca tcgctacgac tgcacccgta
    49141 cgttgccaag atttgacgat ccggatcttg tcttctggcg ttacccgagc atagacagag
    49201 agagctttga cacgcgcatc taattcttcg tctgataatt gatgcagttc tgatcctgaa
    49261 agtgcttctg acggatcatt caagatacct aattctttcg ctatggcact ggcagtcacc
    49321 acatgatctc cagtgatcat gactgttttg atgccagcct ttttcgctcg cttgattgct
    49381 cctttacttt ctggacgtgg tggatcgatc attccgatca gaccaatcaa acgtaagtct
    49441 ttttccagtg cttcagaaga aatatcttgc ggttcttcgt cataagtagc ataaccaaca
    49501 gcaatcactc tcagtgcccg tttaccaaag cgatcattaa cgatcgccgc ttgttctaca
    49561 tcgcctgaac taaagcgcgg agcaagaaca tcgaatgctc ctttggtgat tgagatgtat
    49621 ttttcgccca tttggtgaac tgttgtcatc atttttcgtt cagaatcaaa aggaagctcg
    49681 tttactcgtg gatatttttc ttctaattct gcctttgtat gataattctc ttcaacagcc
    49741 cttacgatgg ctacttctgt tggattacct ttgataacaa ggtcgtcatc ttcttgctcg
    49801 accgtcacat ctgtacatag tgcagccatt ttcaatattt ccatcgcgct gttcgtcatt
    49861 gaatcttcga tattcgttac ttcatcttct ttgctccata cccggcggac gcgcatttca
    49921 ttttgtgtca gcgttcctgt tttgtctgag cagataacat tagcggttcc tagcgtttca
    49981 actgctggca actgtcggat gatcgcatgt ttcctcgcca tcttctgcac accgtatgca
    50041 agtgtcaacg taacaataac agtcaaggtt tccggtacag ctgcaacagc caaggaaatc
    50101 gaagtcatga acatctcaag gagtggttct ccttgcagtt caccaatcac aaatactaaa
    50161 gccgcagccg ctaaagcgat catactgatt cgtttgccta gctgattcaa tcggacctgt
    50221 aaaggtgttt tttgcatagt attttcgttc aaaagatccg cgatcttacc catttctgta
    50281 gccatgccaa tggcagtgac gatcgctttc ccacgacctg cagcaaccgt acatccttta
    50341 aataccatat taagttggtc acctaatgac tcatcttctt cagcaataaa tgctgcatct
    50401 ttttcaactg cttcactttc tccagtcaac gccgattctt ctacacgtaa ttgtgatgct
    50461 tcgatcaaac gggcatcggc agaaatagca tcaccggact ctaaaacaag aatatcaccc
    50521 actaccagtt gctctgactc aacagtggtc actttgccgt ctcgtatgac agttgccgtt
    50581 tgtttattca tatcttgcaa ggattccagt gctttttctg cgtttccttc ttggacaatc
    50641 gccaataccg agttgatcac aacgatagcg atgatcagca gtccttcaaa taaatcccca
    50701 tgttctgtag caaatgcggt ataaaaggaa atcaccgctg ctacaagaag gatgatcgtt
    50761 gtaaaatcgg acaagcttcg aaagaatttt ttgatcatcg attctttcgg cgcctcatcg
    50821 aatttattca atccctgctt ttttcgccgt tcatccactt cttgtgaaga caacccagta
    50881 tctggatctg cttctgttac cttctttact tcttctattg tctgcttgta ttcttccatc
    50941 ctattcctcc tttaaaatct tcgtacaaaa aaaggtactt attttactat aacatccttt
    51001 ttttatttac ttcaaataaa gggcttatcg attatttgtt aagaaagcgt aaaatttaaa
    51061 gtgcttatat ttgcaaacgc taccaaagga tgctattata tagatgtaag gaaggaggat
    51121 accatcaaat agacagaaga atttctcgat agaacagtgt aagcaaacaa agttgttaga
    51181 agtacaggtt tcacccagac atgcgtgtag agtacaaacc tataaatagt gaatgcgtat
    51241 ggatgtttgg atcacgaagt atccgaaaca agaagtaatg cgatacgtaa caattgaata
    51301 acgtaaacgt ctgaaaattg aataggaaca agagatgttt caaatgttga agatgtaacg
    51361 aaaacaaaat ttttgtttaa catgaagaaa ttcaacaccg tgctttgcat gtatgatgga
    51421 aagttacaat catttctgtt tatttgaatc cttccgaggt acaagaaagc cgagcttatc
    51481 gagagctcgg ctttttcttt gtcataaaat ctttgtaaag cttttttgtt attcaaaact
    51541 agcggctttt cataacctta tttcacgaac atcgcatctc caaaactaaa gaaacgatag
    51601 cgctcttcaa tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta
    51661 actaacatga ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc ttctaccact
    51721 ttgaattcat agccaggcgt aatgaaaata tcagtccatc cgctgtctgc tttgatttct
    51781 ccattaaatt tcgttccaat cgtttctaat gttcgaattg aagttgttcc gacagccacg
    51841 attttcccgc catttttacg tacttggttc aatcgctcag ccgcttcttc cgtcaaacgg
    51901 tagaattcac tatgcatatg gtgttcttca attttatcta cgctgacagg tcggaatgta
    51961 cctaacccta catgtagtgt cagatagact aattcaacac cctttgcttc gatctcatca
    52021 agtaattctt tcgtaaaatg caatccagct gttggcgctg cagcagaacc attttctttc
    52081 gcatagacag tttgatagcg atctgggtct tccagacgtt ctttgatata tggaggcaat
    52141 ggcatttctc ccaatgattc aagaatctct agaaaaattc cttcatactc aaagtcaatg
    52201 atccgtccgc cgtgttccaa ttcttctttc actactgctt gcaatctgcc atcaccaaaa
    52261 gagattcttg tcccaacttt ggcacgtttc gcaggtttga tcaatgtctc ccacgtatct
    52321 ccttgtgtat tcgttagaag aaggacttct aaatgcgcac cagtatctgg tttttctcca
    52381 tataaacgag caggcagaac tcgtgtatta ttcataacta gcgcatcacc aggctgtaac
    52441 tcttcaatga tttcatggaa atgtttatct tccatctctc cagttttccg atccaaaaca
    52501 agtaaacgag aatgatcgcg gtcttttaat ggtgtttgtg cgattaattc ttccggcaag
    52561 tcaaaatcaa aatcttctgt tgttaacata tcgtcactca ctttctatca tgtgcttcat
    52621 cattctatca tgtttttcgt tctgttggca aaatcagcac tttttcactt ctattttttg
    52681 ttttcctaaa taaaaaaact accctatttg tattttttca ttgttttagg catactagta
    52741 atagatagtt cttcattgaa aatagaaaaa gaggtggcac catgattgaa tttcaaaatg
    52801 tgtcaaaaat ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa
    52861 agggtgagtt catttgtttt atcggtacga gcggaagcgg aaaaacgacg acgatgcgga
    52921 tgatcaaccg aatgactgat ccaacacaag ggaaaatttt gattgatgga aaatccatcc
    52981 aagaaattga tccagtggaa ttgcgacgtc agatcggcta tgttatccaa agtattggat
    53041 tgatgcctca catgacgatc cgtgaaaata tcactcttgt ccaaaaattg ttgaaagtca
    53101 gtcaagaaga acggaataaa acagctgaaa aaatgattga tcttgtggaa ttgccccgtg
    53161 agatgttaga tcgctaccca catgaattat ctggtggtca gcagcagcgt attggagtcg
    53221 ttcgtgccct tgcagcgaat caagatatca tcttgatgga cgagccattc ggtgcgcttg
    53281 atccaatcac tcgtgattcc ttgcaagacc ttgtcaaaga tttacaagag cgtttgggaa
    53341 aaacgatcgt atttgtaacc catgacatgg atgaagcgat taaactagca agccgtatcg
    53401 ccatcatgag tgaaggccgt gtgatcaatt tgatacacca gaaaatattt tacgtcatcc
    53461 tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag ccaagccaaa
    53521 tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc acgattacac cggaaaaatc
    53581 attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt tagttaccga
    53641 taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa aacgaggaaa
    53701 agtatccagt gtcggcgaca ttttgaataa acatgttttt tacgtaaaag aatccgccta
    53761 tcttcgtgat accttgcaac gcattttaaa acgcgggctg aaatacgtgc cggtcgttga
    53821 tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata tcgtctacga
    53881 tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagcta ctcctgaaga
    53941 atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag aaattctctc
    54001 aaaaggtttt gaacatcttt atatttcagg acttgctttg atcattggta tcatcattgc
    54061 tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg tgattggctt
    54121 aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga tcccgctatt
    54181 tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat tgccgatttt
    54241 acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg tggcaaaagg
    54301 catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg ctatgccaac
    54361 gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta cacttgcctc
    54421 ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata attaccaacc
    54481 agatctcatt ttggcaggta cgatccctgt aacaatttta gcactattgg ctgattggct
    54541 tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga atgaagaatg
    54601 aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc gagttgttcc
    54661 ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg tggaatcact
    54721 tcagaagcac agatcttagc aagtctagtt gccggaatgg ttgaacatta tacagataaa
    54781 aataccgcaa tcatcaacaa tctggctact acaactatca accatcaagc gatgatgaac
    54841 ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac tacattgaac
    54901 cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga atttgagaaa
    54961 agatacggtc aaacgtggtt cccgtcctat agttttgaaa atacgtatgt ctttcttgtt
    55021 cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca gcgatctgaa aaatgtagcg
    55081 gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga cggttatgac
    55141 ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca gattggactt
    55201 gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc tacggatgga
    55261 cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt cccaccatat
    55321 gatgctgctc cagttgtaga caacaagata ttagctgata cgcctcattt aaaagaagcc
    55381 ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa ctacgaagca
    55441 gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga aaaccactat
    55501 tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt ttctattatt
    55561 tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg atttctatct
    55621 atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt tctaggagac
    55681 gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc ccttctctag
    55741 ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg attgttactg
    55801 tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg atccaagtag
    55861 acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa cgactattca
    55921 tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac gcgttagttg
    55981 ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt ggagatatca
    56041 ttatccgagg aaccaatgca acagagggca catcgattat tttagcaggt gcattgccaa
    56101 ctgcattaat ggcgatcatc acggactggc ttttaggtat tttggaaaga cggctagatc
    56161 cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg cgaccatttg
    56221 ttggtcgcaa aggctttttt agttctgtca tttttaaggc aataatccac tctgattctt
    56281 ccctccttat gggcaaagag ttgcttttct tagctattta tccattccgt atactccaac
    56341 ttcatactgc caacgggttc atatctgcat ccaccgacta agtttttttc ttatccgcta
    56401 aggcattttg ccaatccatt ttcccatcac tcgtcatgga attcatacca catgatctct
    56461 aaatactcgt ctgcttgttc tttgaattgg gcatcaattc ttacatcctt tggcgtggct
    56521 ttcatttaag ccttttccat cagtcctcga ttgttcgatg ccttcagctg catcatcaaa
    56581 actggtgact gccgtatcgg taacttcgat catcttttat acaatcggga acttgccatc
    56641 ctcatccttg gctaaaaaca tatctagcca ttccagcttc tcgccatggg aggtttgcgg
    56701 ttcctaaaac cggtacacga ttcttatgga ctgcatccgt cgcaactaga cttggcgata
    56761 cgatgatcct tcaccagaag agcacccccg taaaccaatt gatcgatata ttgccaataa
    56821 gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg cattgccgtt gatactgcta
    56881 ttcatggccg aaagcgccac gactttaact tcttccgttt gagtgacatt catgttcggc
    56941 aatgtcgctt tggatacccg ctctgctaaa ggaacgacac tggtattgta tttcgtatct
    57001 gtatcttttc caaaagacca tgccaacgaa tcctctggga accaataaga cgactccggt
    57061 tgattgtcca ttgctttttt caacatactt ttggctgttt ctcgataaac gacatttcca
    57121 attgtcttcg cctcactttg gcgataatcg atggtcgttg ccactgccaa cactaacaac
    57181 gatgctccaa ttttactgat tttccttgtc acacttcttc ctccttgttt tacataaacc
    57241 cgtcaacatt ccattcctaa actcaaacaa tgacccttca aaggtggaga tataggaaca
    57301 aattaaagaa atcatgcaat aaatccaaga aggttggttt tagaacaaaa aaagagtctc
    57361 ctttttgggg agactctttg ttaactatct aaaaaggttg gctcttctca cttcactcat
    57421 aatgtgtcca ggcagaataa atttctacaa ctttattttc ctcatcaact ttgtaaacca
    57481 ctcgatgctg tctattcagc ctgcgtgagt atctgccttc atgtgtaggt ctcagctttt
    57541 caaaagactg cgtgggcata tatggatctt ccttcaatgt ctgaatcact tcttcaaatt
    57601 gtttctttaa attcgtttgc ttgatttttc ttaaatccac tttcgcagag tttttgattg
    57661 cgactgtata gttactcatc aaagagcttc ccaatcaata tcatcgatat tcgtaaagct
    57721 actgtcgtct ttttcgcggt ctcgtacttt atctaaagta cctgtttgct caagaaaaag
    57781 ggtttcttga atcgctctcc agtcttccag cccaatcaat acagcattgt ttccactacg
    57841 atcactaata atctcgattt cagtatgatt ttcatttacc tctttcaata actgataaaa
    57901 atttttacga gcagtgctag gattcatcgt tgtattagcc attgtctctc ctcctttttc
    57961 tgatataatt atagcgtact tgattaagta cgtcaaaaaa atacaataaa gcacttaaat
    58021 tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg gaggatcttt
    58081 gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa gtaattggta
    58141 acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg caacaacacc
    58201 aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata acctgaggat
    58261 acgcacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag tcttctactg
    58321 cctcaaaaat gattgcctct ttcgtctcct gtcattcatg ttcactatcg atttgatact
    58381 tgcttagacc gttgggtttg tgaatcacca attaaaaatg gcactgagtt atggtaacaa
    58441 aaaatggtca cttgacgagg ctgagattcc tacttatgaa gacacatggc acttaagagt
    58501 cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca aaggattcgg
    58561 aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa gaataataac
    58621 atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc tgagaataag
    58681 acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga ttttaaaact
    58741 tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca cgccaagaca
    58801 atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag cgtctgtttg
    58861 gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca tttttactta
    58921 cgatatctac ataacggata attggatttt caatcgagcg ataagaagag tggacgattg
    58981 aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt tctttttcga
    59041 catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa cgtgcgtaat
    59101 tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg gtattcccgt
    59161 cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact tttttataat
    59221 gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag aaaggccaaa
    59281 tgtcccctaa ccgatagaca aacaaaatcg caatgattgc atatgagatg aacgcacccg
    59341 taatattggc aatcgatttg cttaaccatc tttttccttc ttttttccat ttgacgacca
    59401 tccctgtttg agataaagca aatggaatga acacgccgat agagtataac ggaatcaagc
    59461 gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct agtgtgataa
    59521 tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc atgaatttat
    59581 cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta gcagcaactg
    59641 ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt ccaaagacag
    59701 ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca taccaatagt
    59761 tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc gtcaatgtag
    59821 cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta atcgcctcca
    59881 ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg atagaaacac
    59941 ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct gtcacgatat
    60001 tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac accggaaata
    60061 ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt agcaaaacga
    60121 ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat gtgatcgctt
    60181 cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat aaagagccac
    60241 cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat gctcccccgc
    60301 catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc aataaaataa
    60361 taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa gcgactaaaa
    60421 cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac gacaataatg
    60481 ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt aaaggcttac
    60541 ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca attttacttc
    60601 aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca ctgaccttaa
    60661 aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa gcgctttttc
    60721 aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt tgaattctat
    60781 aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat atcttaactt
    60841 ttggaagaga gatgtaaggc tgctttctac ccatcattct tactactcga agcatagcgc
    60901 agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt aataagccca
    60961 accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact tgatgcagag
    61021 cagctttttt acaacctctt ggtacacggt gttcaatcag ctgaccctcg gtattagctc
    61081 aaaaaaacgg caaaatatga ggaactattt ttccattttt ttctctaata ctcaggtcaa
    61141 aacactgatt tcacaacctc tttttttaat gaaaaatatg gctctcatgc gccgcttctt
    61201 tgaatttcgt cattttttct tcgatgaacg gatagattgc cgttcctacg attccaaaga
    61261 caacaaacaa accgatcatg atccagattg ctttcgtagc atttggccaa taaattcctc
    61321 ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg caagaacgga ttgatcattt
    61381 ggaagaattt ccctgagact tggatcggat agttcccgcc tccgccagag atcgacagta
    61441 ctaagatgat aatagcgatc cctttaccga cattatcgaa taatgcagcc aagacataga
    61501 cgatcatcat gaacgctaag gcgatcaaga cagcaaatga cacgctgtag accggatttt
    61561 ggacatctac tcctagtaag aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg
    61621 ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta aaattgtttc
    61681 ggtctttctt tcctaaatag tattctgtcg tagcaacact tgaaagaaga acagcgccga
    61741 cccacagaca tagtgcagta taaaacgggg tgctagctga accgttattt gcgattggat
    61801 aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct tttgttgcgt
    61861 ctagtttcaa taatttgatg acttcgccta gatcgacttc ttgttcgcct tttcggatcg
    61921 cgtctgctgc tttttggata ccagctttga tatttggcca gtcattgttg atcaagtccg
    61981 cagcagcgtt cacagctgtt tcgacttctg gcattttttc atttactgtt gtcaatgtac
    62041 cggtgatttc attttttagt gctggccaat cattttggat aaaatcagcg gccaatccta
    62101 atttttgctg aatgatcggt aattcattgt tgtataggtc tgccccttca ttgatgccat
    62161 tgacgatggt atccatattt ccattcagca ttgtattggc atcatggatt tcttgtccaa
    62221 ttgcaggcat ttctttttga tatttttcta aaattgccac tgcattcgtt acagttgctt
    62281 tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc tttgctcagt gcgccttgtg
    62341 cagtcccaat cgtatcgatg acttggtcta gtagagaagt gacagtggaa ctgatttgac
    62401 ctgtattgat accagcaatg atgccattga tattattaac aacggcatca atttgatcta
    62461 gcaattcatc aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc
    62521 ggttttgcag attgcttagc aagggtttta attgatttag ctgatcaata attggctgta
    62581 atttcgtatt tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg
    62641 cattttgttg ttgagatatt ccgtcaataa agccgttcaa tacattgctg atgtttttct
    62701 tttcatcatc agtcagctta ccatcttcaa tagctgttct tacagtatct attaacgaac
    62761 ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac atctacactg tttgtgatgc
    62821 ttggtatcgc atctttcagc tgagaggcac ctgattttgt ttggtttgct agatcacttg
    62881 cctgattttc taaatttttc acatctggca agatatcttg tacttgttgg atgatcgtca
    62941 agccttgttt tgcttcatcg attccttggt tcattgtctc ttggatcgaa gcaaaatcgc
    63001 cgtcgatctc tgctagttgc ttaccggcat tttggatctc tgggattttt tcttgcaaat
    63061 caaggatgat ttttgcttga tctttcaact caggcatttt gtcattcaat gcaacgactt
    63121 tttcacccat ctcatctact ttagggatgt aatccacgaa ttcatttgct ttattcagtt
    63181 tttccttgat ttcaggtagt tgactttgta attcgaggac ttgttttgtg tagccgtcga
    63241 tcgtgtccaa attttcatct gttgataaaa tcatatcttt aactttattg atacttacta
    63301 ggttcgtatc gatgtcgtaa cctatctcat tgaatacttt caataaagtc gaactggctg
    63361 ttttgataaa ttcatttgtg atttgcgatt ggatcgaact tgctccttta tcggtgattt
    63421 tcggtgcaat cgcattgatt ttttcattta ccgtatattc gatttttggt ttcttgatat
    63481 caccactagt aaaactcagc agatcttctg agaaatcctt tggcaagtaa atacctgcgt
    63541 aatactttcc ggaacggact ccgtcttcta attgttcttt tgaatcaacg aattgccatc
    63601 ctaattgttt atttttatga agcgattcga tgacttgctc cccaatggcc acttctttac
    63661 cttgaaactc tgctggcttg tctgcactgt aaacagcaat tggcagctct ccagtatttc
    63721 catatggatc ccataatgct ttgatgttaa accacgcata tagcgaaggg atgatcatga
    63781 tcgcgatgat cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat
    63841 ataatcgaag cgtatttttg attgactgca tagttctccc ccttcttctt ttttcagctg
    63901 aattacttta ccatgtattt catacttttt tcaatcggct tttttcaaaa aagtcgaatt
    63961 tttggacata tcacggcaat tgtctcattg taatctctta catattgaga aataattaag
    64021 gattacagcc tttactttat catgaaataa aagggaaaga taattttatg ccaaagtttt
    64081 tatctattat acgtatacgc aaaaaagcta aaacattttt tgtctttgcc cctacaaaaa
    64141 tgtttcagct tttttcgttt tttattcctg tatttcttat ttttcgaatg acaggatgat
    64201 tttcccagca ggaggatgtc cttcaactag ctgatgtgct tggatgacat gcttaagtga
    64261 agcagacaga ttcattgaaa taaaaacatg aaaggctcct ttttggtagg ctttgattat
    64321 gccagcaaag gcttctgcat ctagatattc ctttcttgga acaaacgatt cataaaagcc
    64381 ttcctttttt tgccgcaaat ctagatctgg gaggtcattt aatgcaacat acctgccgcc
    64441 tggtttcatg atctggatgc cggtttctcc tttgatactt cctttcgtcg catcgattac
    64501 aaggtctgcc tgatcagcaa attgcagacc tggatttgtt ttatcataag cagcaaatgc
    64561 tgaagctcct aattttttta ccttttcctc atttttactg gatgcactgg ttaagattcg
    64621 gattcccttt tcatggagaa gctggatcag acttgacccg accgcacctg atgctcctag
    64681 tatcattaca gtatctgtcg gctggatttc cgtcaaatgg ttgattagat tataagccgt
    64741 aattcctggc gtgaccattc cggctgcttc ttcccaactc attttatctg gaatttttgc
    64801 tacttttgct gatggcaaca cgacttcttc tccgtaagtc ccgccgactg catggacagc
    64861 tactcgatca cctacatgaa cagttgtgac atctgaacca atctctgtca caataccagc
    64921 gccatcatta cctggtacat aagggaattt caatgttcgt atctctttca tttctcctaa
    64981 acgcaaggca acatcataag gattcacact gaatgctttt atttctacac gaacatgtcc
    65041 agaaccgact tctctgggat gagcatcgat ttcttcaaaa acatcttgtg cttctccgta
    65101 accgttaatt gcaaatcttc gcatggtcac catctccctt tttctttaag tttacgttta
    65161 tgcctttatt ttgacaaaaa aaatgtcttt tcgttttggc gcatgattca aaacaaaata
    65221 gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa aagcgtaaag attacgattt
    65281 acgctaataa aaaataattg gaggaatttg ttgatggcta aaaaatcaaa gattgccaaa
    65341 gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt tacgccagga attaaaagcg
    65401 caaaaagatt acgaaggctt gcgtaaactt cctttagatg ctcgtccaga acgattgaaa
    65461 aatcgtgatt taatcgatgg acgccctcgc ggatacatgc gtaaattcgg gatgtcccgt
    65521 ataaaattcc gtgaattagc acataaaggt ttgattccag gagtaaaaaa agccagctgg
    65581 taatccaagt ctggctgtaa tgtaaaggag gagggcgcat ggaccactcc ttggatcatc
    65641 aaaaaagata cgttcgcttc ttctagcaga aatgctggta ctagaagcag acgtatcttt
    65701 ttttacgcac agctggtcct tttttatatg taaaggctgc atggttactt cgctatattt
    65761 atttagtaac aggagtcagt gcttcgatac ccacactaat tttcaatgct cggtcaactt
    65821 ctgccataat gggtcgatcc aaatggcaga ccttttcttt caatcttgat ttatcgatcg
    65881 ttctgatctg ctccaacagg ataacggagt ctctttctat tcctgttcca gtagctttga
    65941 tcccaatgtg agtcggaagc ttaggtttag ccatttttgc cgttatggcc gcaacaatga
    66001 tcgtcgggct aaaatggtta cctagattat tttgaataac caaaactggg cgtgttccgc
    66061 cttgctccga tcccacgact ggagaaagat cagcaaaata aatatctcct ctttttacca
    66121 taggatcctc cttattcttg atattctctt ggtatccgct gtcctaatcc gcaagccact
    66181 tcatagtgga tcgtacctaa ttgatctgct atatcctgca tagtgatttc tttgtctgca
    66241 tttttgccga taagcgtcac ttttgttccg acaggaaatt cttgaggcaa tcggatcatc
    66301 agttgatcca tacatacacg cccaatcgtt tcacagtagt tcccttctac caatagagaa
    66361 aaaccctgca tctttcgtgg ccagccatct gcataaccga tcggtatcgt tccgatccac
    66421 tctgcttcag gagtgatata tgtttctcca tagccgattc cttcacctgc tgggagtttt
    66481 ttgacttgaa tcaattcaga aaccagctca agtgctggct gcaatggata aacttccggt
    66541 agtgcgtgcc cagagggatt aagtccgtac attgctactc cataacgaat catattgccg
    66601 attgtttcat catgccataa agccgttgca ctattgctga catgtacata tcgaggaagt
    66661 tcgggaagtt ttttcagcac ttctataaag cgttcttttt gcagattcca atatgtatca
    66721 tctgcctgat ctgctgtaga aaaatgggta aataaacctt cccattctaa tacacgactt
    66781 tctttgatga atgcagctgc ctcttttact tcttcaggtg tacaaaatcc gatacgaccc
    66841 atcccagtgt ctgcttttag atgaatcgaa agaggcgtct ctagcttact tttctctaaa
    66901 acagcagctg ctgcttgcaa ccattcccgt gtacctgcag ttactgataa atcatgagca
    66961 acagctacag tcagtgcgtc gtaaggaacc atattcaaga taaggatggg ctgagtaaat
    67021 ccagcttctc tcaactcgac tccttcatct aaatttgaaa cacaaaagcc cgaagctcca
    67081 cctgcaactg ctgcctcagc tgtttcaata gctccatgac cgtatccgtt cgcttttact
    67141 actgcaaaca attcttttcc ttgcggaagt cttgcaactt catttgctac attttcagtt
    67201 atagcttttt tatgtatcac tgctttcgtt gggcggtgcc aagcgacaac catcgttttt
    67261 ccttctttct agtcttccaa aataatttgt gcaaaagctg tttcatttgt atgagtaata
    67321 ctgacccata cattgccttc atgaggtgaa cgcgtcacaa caggctgacc attttcattt
    67381 tttaaaatct cgacatcttg aaaggtaact tttccgatcc cagttcccca agctttagag
    67441 aaagcctctt tgcaagcgta acgcccgcct aaaaattcta cttggcgatg aaaaggcaag
    67501 gactggaata gtttcatttc atcagaggtc aagattcgtg caatgaattt tggtttttct
    67561 tcgatcagcc gagtgatcct aggaagttcg actgcatcga tcccaattcc ttttatcata
    67621 tgcttttctc ttttcaataa atttagaaca ctctcattct accaaattat aaccattttg
    67681 ccacaaataa gtccacaaat aacaaaaatg aaagtttctc ttaaaaaaga gaaactttca
    67741 ttttatgttt aatcgttatt tgaacggatt acaaatccgc gttttttatc attgtgacgt
    67801 ttaccgccat ctttttggct gttattattt ttgctataac cgccttttga tttgtttcca
    67861 cgataattac cgccgtcacg acgattacgg ttgtttccgc caccacgttt gccattttta
    67921 ttgtatcctt ttttctgcat tggcaatggg cgttctggtg tgatcttcac tggcactgca
    67981 tcagctggat cttttgctgt cgtttttagc aaaagtgcaa ctaaatcttg tgcagaatat
    68041 tcttctaata atttatctgc tgtttgcagg tatttatcta gaccattttc tgcaagtttt
    68101 gtttcgattt gttcaactgc agcacctaat tgacctttga atgcttcttt ttcggttggt
    68161 ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa tcacatgcaa gtagtccatt
    68221 tcatttggtg taacgaaagt cacagacata ccgccttttc cggcacggcc tgtacgtccg
    68281 atacggtgaa cgtagctttc tggatcttga gggatatcat agttgtacac atgagtgaca
    68341 cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta caaggatatc taaatgaccg
    68401 ctcttaaatg aacgcaatac gctcattcgt ttttgttgag acaaatcgcc atggattcct
    68461 tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt cgtctacacg gcgttttgta
    68521 cgtccgaaga cgattgttaa ttcaggtgtt tgaacatcaa acaagcgagt catgatatcg
    68581 aatttttcgt attcttttgc gcggacatag tattgatcga tcaagtcagc tgtcatttct
    68641 ttcgctttga tcttcacatg atgtggattt ttcatgaatt taacgccaat attcttgata
    68701 gctggcggca tcgttgctga gaaaagtaat gtttgtcttt gatcaggaac ttgtgaaatg
    68761 attttttcga tatcttccaa gaagcccata ttcaacattt catctgcttc atccaaaact
    68821 aatgtttgaa cggttccaag tttcaatgtg tggcggttaa tgtggtcaag catacgtcct
    68881 ggtgtaccca caacgatatg tggacgatct ttcaagccac ggatctgacg gccaatatct
    68941 gctccgccgt aaacggcttg tacacgtact tttttgtcgc gccctaaacg atataattct
    69001 tcttgtgttt ggatagctaa ttcacgtgtt ggtgcgataa ctaaaccttg taattcgtga
    69061 cgatctggat cgattttttc taacattggt aatccaaatg cagctgtttt ccctgtacct
    69121 gtctgtgctt gtccaataac gtcttttcca gctaatgcta gtggaatcgt ttctgcttgg
    69181 ataggcgttg cttcttcaaa accagcgcgt tcgactgatt tcaacaattc tggtgataat
    69241 tctagttctt taaatttcaa atgaatcctc ctatatggtt gttaactgag gatgtagctt
    69301 ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt ttctttttga cagtggccac
    69361 gcacgttttt atccattctt agcccctgtg gcaccaccga aaaatcactt tgcgaagttt
    69421 aggcattaga aaagccaaac gggtcgcgtt cagctttatc attattatga catctggctt
    69481 caacaagttg tttctatggc aaaaccagtc caatttctct tcatctttcg gcaataggtt
    69541 attctaacat agtttttcgt tttctgcaag tttacagatt tttatttcat ttgctgaagt
    69601 tgttctacca cttcaaataa gcccatgctg ttgctgcctt ttagcataat actatctgtt
    69661 gcttcaagat tttgtttgat agcatcaatc aatttattct tttctttttc aaaatagaac
    69721 acaggtagtt gggggtattt ttggttcaat tcttctttta gtgcatgcat ttgttcccca
    69781 taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt cactcatttg cgcatgcata
    69841 tttaaagact ctttccctaa ttccagcata tctgctaaca cagcgattct gcgtcctttt
    69901 gttggcagat ttttaaaagt atctaatacc agtcccatgg ctgttggatt tgcattgtag
    69961 acatcgctca aaatagctgc accatttcct gccgttagcc attcggttct gttttgagtg
    70021 atagaaacat gggcgagccc ttggcgtatc tcaggtatgg acagtccaaa gaagcgcccg
    70081 atcgtatacg caatcaaagc attcgttaca ttataacttc caggcagcgg gatcgtaaat
    70141 gtttctcctt ctactacaaa atcggtttgt gcttttcctt ttgctgtcac ttccgcatga
    70201 acatcgccat ttccaatacc gaatgttgtg actgtttgta ctaatgttgc cgttaaaggt
    70261 gtcagcaacg gttcatctgc gggcacaacg agtaatccgt ctgatacaag tccgtcagtg
    70321 atttccattt tggctttagc gattccttct cgcgacccaa gattttctat gtgtgcttca
    70381 ccgatcattg tgattgctgc agcttttggt tgagccataa gtgaaagttc cgtgatttca
    70441 ttggcgtgat ccatgcccat ttccaaaacg atcttttctg tatcttgggg catgtgcaaa
    70501 atcgtgtaag gaagaccaat attattgttg taatttcctt gcgtcttata tgttttgtat
    70561 ttttcagaca agacagacgc ggttagatct ttcgtggtgg ttttaccatt gcttcctgta
    70621 attgcaacga cttctgcgcc tactttttcc aagtaatagg ttgccaattg ctgcatggct
    70681 ttcaaaggat ctgctacagg aataactgga aaattttttg gagcttgttc aacaggcaag
    70741 ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct ggataaattc atgtccatct
    70801 cgcgtaccag caagaggaac gaataaactt ccttcggtta tcttccggct gtcaaattcg
    70861 acattggtaa tctttgtcag tgcagagcat tcgctgccca cggcttgtgc aatttcttgt
    70921 attgttagat tcattgagat ctctcctaaa aaaggttgtg aaaaaagcgt ttggcctaaa
    70981 cggcgggttc gcatgactct cacttccaag taaatttctc tattcgtatt tagaagctag
    71041 aatgaacgcg gttacgcaat cactccagcc tcttctttat ttctctttat tcattttttt
    71101 caaaaaaaga ttgacgctga tggtatcgat tcattcctaa ctggatcagt tcttcaatca
    71161 aatcaccgta tttcaagccc atattttccc ataagagtgg gtacatactg aactccgtaa
    71221 atcctggcat agagtttaat tcattcagga ataattcatt tttatttgtc aaaaagaaat
    71281 cgcaccggct caatccgctt ccacctaaca tcgtgtaagc taacttcgcg tactcttgcg
    71341 ctttttgata aacttcttct ggcacttcgg ctggaatctg catttcgatt ttattattga
    71401 tatattttgc ttcataatca tagaatgcta cgtcttttac gacttcacca ggcaaagtcg
    71461 tccgaacgtc ttcatttcct aatacagcaa cttcgatttc gcgcgcttca attccttgtt
    71521 caacgattgc tcgagaatca tactgatagg ctgttgctaa agcattttgc agctcttctc
    71581 ggttttctgc ttttgtaatg ccgacactag aacccatatt cgccggtttg acaaacatcg
    71641 gataaagcaa agaaccttca cattgatcaa atactttttt aggattttct ttccattgat
    71701 tcttaagtac tggtacataa ggaacttgcg gcacaccagc agcttgtaaa atatacttgg
    71761 tcatgatttt atccatggca catgcactgg tcaatacgcc tgcgccgaca taaggcatat
    71821 tcaatgtctc taagaagcct tggatcgttc catcttcccc gtttggccca tgtaaaactg
    71881 gaaaaacgat ggctccttct tctttgattt cgcccggatt gatcactttt cctgtaaagc
    71941 cttcctctgt ctgtccactt gggtcccatg aaagatgcaa gacatctttg ctggcaggtt
    72001 tttctgttag taatggacct ttgacccatt gtccttcttt tgtaataaat acgagttgaa
    72061 cttggtaata attataataa atggcattta aaactgaaaa tgcggaaaga atggacactt
    72121 catgctctgc gctgcgtccg ccatatagta aagtaatctt caaaagattg tcctcctgta
    72181 ttctaaatcg ttcagtgatt tactctcata ttttagcaca aaacaaaaat aaacgaataa
    72241 cttatttata aaaaaacgag ccagcttcta aaagaacgag cgtcatttat tttttgtcag
    72301 ctagattatt tgtcccgttt tttataaaaa gttatgataa agaaaaggag ggatgatgat
    72361 ggcgcaaaca aatccacgca gccgcttggc agaaatcatt cgagtgttca tcaattacaa
    72421 tgtagtgcca aactttgtac agcaaaaaaa tcccgaacag gtaaaaaaag cctttgaaga
    72481 actaggcccc accttcatca agataggaca gatgctttct gttcgagaag atttgctttc
    72541 ttctgccttc actcaaacat tcaaaacatt acaagatagt gtacctagcg acacattttc
    72601 aacggtcaaa aagacgatcg aaacagaact ttctctttca ttatcagata tattcgatga
    72661 tttttcaaaa tcgccttttg cctctgcttc tatggggcag gctcatcgtg ccaagctgaa
    72721 gaacggtgac gcggtagtcg ttaagatcca acaccctaac atcgccgaag agatccgact
    72781 tgatcttcaa ctatttgaac gagcgatccc tttgatcaaa tacattcctg aaactagcgt
    72841 agttgatcta aaaggggtac tccaagaagt aaaacgttcc ttgatcaatg aaatggactt
    72901 cttaaaagaa agtcaaaatg gcgaacaatt ctatcaaaag aacaatggct ggaaagagat
    72961 ccgcagcccc aagatttatg acgctttttg ttcgaaaaaa gtgatcgtga tggaagaaat
    73021 gtctggtaaa aacttgaatc atttaatgaa cgccgaaaac aaaactgaaa cttttatcac
    73081 tggaattcaa aacaagcagc tgaaacaaga agttgcaaag cttttagttg agaactttat
    73141 gaaacaggtg tttgatgatg gttttttcca tgctgatcct catccaggga atctgctatt
    73201 ccatgtacta acaaaagaag aacagacaca agctagccga aaaactgaaa cagtccatga
    73261 aaaagagttc ggttcctttg cttttcgagc ctccacttct gcagaagacc ctgttgcgcc
    73321 atataccatc aactacatcg attttggtat gatggggcat ctttctgcag gacttaggca
    73381 aaaactcaca caagccgttt tagcgctgta tacgaaagat gcttatcgaa tcgaaaaagc
    73441 ggtccttcga ctttgtcagc aagaaggctc gtttgatgaa agtcggttcc accaagaact
    73501 gacaagtttt cttgaacagt attatgattc tcctattgat gaaatcaact tgcaagaagt
    73561 attcacacat gtcgtgacga tttgtcatca aaataatctg cagttcgatc gtgatatcac
    73621 gcttttgctg aaagcatttg ggacattaga aggggtgatc cgcgtcctcg atcctgaagt
    73681 ttccctcatg gaagtcgctt cgccttttgc tcaacattat tttctcactc atctggatgt
    73741 agaagataca ttgaaacaaa gcggattaga cttattggaa gggatgaagg ctgcgcctaa
    73801 gatccctcaa cagctccacc atctattaga aatgtggact tcgggacaag gaaaagtcaa
    73861 tttggaattg aaaaagcaag acaaattatt gagcaggatc gaaagtatga tcaatcggct
    73921 tgttttcggt atgattttag ctgcattgat cgtagggtcc tccctgcttg tccaagcagc
    73981 acctgtagaa aacgcagagg tcgtttcctt attagggata tttacgtatg ctattgccgc
    74041 attcgtcatt atttttttgg caatcgatgc attgatccaa atgtataaga agaggaaaaa
    74101 gtagaaaaag gttgtgacag aaagaatcag cttcaagaaa taagaagggg ttttacataa
    74161 attgtttctc aaatttttgt gaaaattcgg cttatttttc gaagaggcta cttctgaaac
    74221 accgagaata aaaaatgtga aaaagctgtt ctgcacccag taataagaac aatcaaaaag
    74281 ggaacatgga acacatttat cctgtttccc tgttcccttt ttgatgtatt cgagcataaa
    74341 taaaatacct tcgttaccta ctatcttctg tacttggtaa accttagtca attgcctttt
    74401 ttaacgcttg acgaaaagac atatgatctt tctccgtaaa gggttttgga ccctttgtcc
    74461 ttatgccagc ttttcttaac tttccactat gataacgttg ttctagaagc gtgccgatat
    74521 tcgcttcgtc aaattctttt ccagagtgat ggaacactcg cacacccttc ggcaatacaa
    74581 gtgaagctaa accataatct tgcgtgatca aaaagtcatt cttcttaacc ataccgacga
    74641 ttttataatc cgcactatca gctccgcggt ctacgtagac gatttggaca tgattagcgt
    74701 attctttggt cgtataatgg tccacactgg tcacgataac cacgtcaaga ccataggatt
    74761 ctcctaattt tatgacatct tcttttactg gagaaccatc tccatcaatg aatagcctca
    74821 ttctttttcg ccttcttcca taaatgataa acagttacta atccgctcag taaaagacca
    74881 acattgatcc attttgccaa gataagcagt tgtacacttt ctatcggcac tgattcatgg
    74941 ttcagcgtga tcaataatag atggattggc tctcccattt cttttgctaa aacaaacaaa
    75001 agcgtcatca ataaaaactg gtggatcaca ctccctttat tttgagtagt atacttgaca
    75061 acgatccggc gatccattgg aatagctgta tcagtttgat aatattcctt atttagccag
    75121 ctgcctgtta ctacgtgcca gaaactaagc agaaagacac ctatcgcaaa accatttgga
    75181 atcaagagga ccaataaaga agaactcagt ccaagcaatt gcatagccaa tggagaccat
    75241 gttaaacgtt tcaaaatcca tggtccgact atttttgctg caatcaagcc tgctgcataa
    75301 ggcaaataca taaaaatcga aagtttttct aatccgtaca gtatgccaac ataaaaactg
    75361 ctaaataaaa aaaggaaatt ccctagcatt ccatttagaa aagtaaacag atttagccag
    75421 cttggcaact tccattcctt cttctggtgt cctaaaacaa acacgaacaa gatgaacagc
    75481 agcaagaaac cataaatagc aagatcaaaa agcctttcat cgaataacaa tcgggcattt
    75541 ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa caagctctcg cgtagctacc
    75601 agatttcttt tgatgtcttt gaaatccagt tcgtaacgcg gataatgact aaccgtatgg
    75661 taggccatga cgtaaaaaag tgtataaata gtaaagcttg ctgcaatctg agtcggtgca
    75721 ggcaactcaa tcgcttgata caggagaaat agccagatag ctgcaaataa gtactttttc
    75781 ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat tgaccgtggt attagcaggt
    75841 acaagccatg ctgcactcag ccctagcaaa acacttgaaa aataaaataa aggaaaataa
    75901 aatacgccta atattccaag caatgaacca gcaccaccaa tcagcagagc gatcatcagc
    75961 aacgtgtaac tatctagacc aaatcgtaga ctgttgacta aaaatagtcc agtcatccga
    76021 aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat tatgcgagtc tgttagtccc
    76081 aaaaataaaa gataaggaat aaaaataacc gtgttactta agataaaggg cgttgtttgt
    76141 gtaaaattac gcccaaatcg tttgagtttt tcgaacatga caatggctcc ttaaacagtc
    76201 ttataactaa tctgctcttc gattttagca ttaaagggga aataaaccaa ttttacagaa
    76261 tatattcttg taaaaaaaaa tacagaaaga agcataagct tctttctgca tttttacggt
    76321 acttgattac tcaagcagac aagcccttct ccacaaaagt cttgcccgac acccctgcat
    76381 ccggcaggtg ataaagatag aaatcaatta tcctgtgcct ccaaaatcaa ttaggagctc
    76441 tgtaaaccag agtccttctc gacccgtcgc ataaatttac tttaacacag ttcctatgtt
    76501 tatgcgagag cttttttcat tttataatcg accatttgaa atccttgacc gatatctgaa
    76561 ataagttctt ctgtaatctc aaatccttca tgcttataaa cctcgattgc ttgatgattc
    76621 ccctgattca ctcgcaaaaa gatctctctt tcctttgttt gcgcagcaag ttcttcatac
    76681 cagcgaaaaa ctgaagaagt caatccttga ccacgtactt tatttagcaa atataattta
    76741 cttaaataaa tttttccatt tatctcttca taagcagtgt aaccgacagt cttttcttcg
    76801 ctttttaata aaaagtagga cacaccttct gtcagttctt tttgaatctg tttttttgac
    76861 tgataagttt tcagcatgta atccacttgt ttttgaccaa tgatcggcac aaaaacttcc
    76921 ggccatactt cttgtatgac tgccatcagc tcatccagtt cattttctgt tacttttatt
    76981 ttttccatac acccacgtcc ttttttacat tataaatgac cttgcgcttg atggaataaa
    77041 aagagcatga tcatgctata cacacccacc gaccacggtt tcaagaaaat cacaatctgc
    77101 ataaattccc taaaagataa tttcgtcaaa ccagctacta gacagatcaa gtcgtctggc
    77161 gcaaagggaa gcagcaaggt aacaactagt acttttttaa tatctttcgt ttttcttgtc
    77221 agcaattcat caaatttctg atactttttt ggcgataaaa taagctggac aaaggctacg
    77281 ccatagtaac ggacaagcaa aaatccgatt gcttccccaa tcactaggcc aatacaacta
    77341 tataaaagcc caaatccatt gccaaacatc agcatgcccg ctactgagga gatccctccc
    77401 ggcaaaatag gaatgatcac ttggatcact tgcaacagaa caaatatcat cactgcatac
    77461 ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg atcgaaaaat cccatgatga
    77521 taagcataaa gaatcaacac aaagaaaaaa aacagaccaa taatgggcat gatctgaaga
    77581 catttttgta aaactgatcc ttttttagac atctatattc cccacatctt ttttcattta
    77641 ttaaaactgc ctcctt
//
LOCUS       DAZKMK010000004        73369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000004 DAZKMK010000000
VERSION     DAZKMK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.004"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..329)
                     /locus_tag="ACG8V1_000273"
     CDS             complement(<1..329)
                     /locus_tag="ACG8V1_000273"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328399.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(343..600)
                     /locus_tag="ACG8V1_000274"
     CDS             complement(343..600)
                     /locus_tag="ACG8V1_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328400.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(795..1337)
                     /locus_tag="ACG8V1_000275"
     CDS             complement(795..1337)
                     /locus_tag="ACG8V1_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0328401.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1358..2449)
                     /locus_tag="ACG8V1_000276"
     CDS             complement(1358..2449)
                     /locus_tag="ACG8V1_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0328402.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2457..4667)
                     /locus_tag="ACG8V1_000277"
     CDS             complement(2457..4667)
                     /locus_tag="ACG8V1_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0328403.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4683..7211)
                     /locus_tag="ACG8V1_000278"
     CDS             complement(4683..7211)
                     /locus_tag="ACG8V1_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0328404.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7263..7673)
                     /locus_tag="ACG8V1_000279"
     CDS             complement(7263..7673)
                     /locus_tag="ACG8V1_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0328405.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7686..7904)
                     /locus_tag="ACG8V1_000280"
     CDS             complement(7686..7904)
                     /locus_tag="ACG8V1_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0328406.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7901..8893)
                     /locus_tag="ACG8V1_000281"
     CDS             complement(7901..8893)
                     /locus_tag="ACG8V1_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0328407.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8972..9883)
                     /locus_tag="ACG8V1_000282"
     CDS             complement(8972..9883)
                     /locus_tag="ACG8V1_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0328408.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9902..10246)
                     /locus_tag="ACG8V1_000283"
     CDS             complement(9902..10246)
                     /locus_tag="ACG8V1_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328409.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10266..10544)
                     /locus_tag="ACG8V1_000284"
     CDS             complement(10266..10544)
                     /locus_tag="ACG8V1_000284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328410.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10903..11403)
                     /locus_tag="ACG8V1_000285"
     CDS             complement(10903..11403)
                     /locus_tag="ACG8V1_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328411.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11404..11949)
                     /locus_tag="ACG8V1_000286"
     CDS             complement(11404..11949)
                     /locus_tag="ACG8V1_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328412.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11946..13202)
                     /locus_tag="ACG8V1_000287"
     CDS             complement(11946..13202)
                     /locus_tag="ACG8V1_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0328413.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13563..13874)
                     /locus_tag="ACG8V1_000288"
     CDS             complement(13563..13874)
                     /locus_tag="ACG8V1_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328414.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13897..15372)
                     /locus_tag="ACG8V1_000289"
     CDS             complement(13897..15372)
                     /locus_tag="ACG8V1_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0328415.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15821..16273)
                     /locus_tag="ACG8V1_000290"
     CDS             complement(15821..16273)
                     /locus_tag="ACG8V1_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328416.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16275..16640)
                     /locus_tag="ACG8V1_000291"
     CDS             complement(16275..16640)
                     /locus_tag="ACG8V1_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328417.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16739..21022)
                     /locus_tag="ACG8V1_000292"
     CDS             complement(16739..21022)
                     /locus_tag="ACG8V1_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0328418.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21169..21408)
                     /locus_tag="ACG8V1_000293"
     CDS             complement(21169..21408)
                     /locus_tag="ACG8V1_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328419.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21498..21740)
                     /locus_tag="ACG8V1_000294"
                     /pseudo
     CDS             complement(21498..21740)
                     /locus_tag="ACG8V1_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21853..22104)
                     /locus_tag="ACG8V1_000295"
     CDS             complement(21853..22104)
                     /locus_tag="ACG8V1_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328420.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22267..23226
                     /locus_tag="ACG8V1_000296"
     CDS             22267..23226
                     /locus_tag="ACG8V1_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0328421.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            23396..24076
                     /locus_tag="ACG8V1_000297"
     CDS             23396..24076
                     /locus_tag="ACG8V1_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0328422.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            24166..25200
                     /locus_tag="ACG8V1_000298"
     CDS             24166..25200
                     /locus_tag="ACG8V1_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0328423.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            25214..25549
                     /locus_tag="ACG8V1_000299"
     CDS             25214..25549
                     /locus_tag="ACG8V1_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328424.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            25533..28013
                     /locus_tag="ACG8V1_000300"
     CDS             25533..28013
                     /locus_tag="ACG8V1_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328425.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            28069..29628
                     /locus_tag="ACG8V1_000301"
     CDS             28069..29628
                     /locus_tag="ACG8V1_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0328426.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            29625..30989
                     /locus_tag="ACG8V1_000302"
     CDS             29625..30989
                     /locus_tag="ACG8V1_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0328427.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            31160..32230
                     /locus_tag="ACG8V1_000303"
     CDS             31160..32230
                     /locus_tag="ACG8V1_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328428.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            32245..32913
                     /locus_tag="ACG8V1_000304"
     CDS             32245..32913
                     /locus_tag="ACG8V1_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328429.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            32933..33511
                     /locus_tag="ACG8V1_000305"
     CDS             32933..33511
                     /locus_tag="ACG8V1_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0328430.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            33912..34823
                     /locus_tag="ACG8V1_000306"
     CDS             33912..34823
                     /locus_tag="ACG8V1_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0328431.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(35201..36118)
                     /gene="rnhC"
                     /locus_tag="ACG8V1_000307"
     CDS             complement(35201..36118)
                     /gene="rnhC"
                     /locus_tag="ACG8V1_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0328432.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            36320..36757
                     /gene="zapA"
                     /locus_tag="ACG8V1_000308"
     CDS             36320..36757
                     /gene="zapA"
                     /locus_tag="ACG8V1_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0328433.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            36815..37363
                     /locus_tag="ACG8V1_000309"
     CDS             36815..37363
                     /locus_tag="ACG8V1_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0328434.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            37478..39838
                     /locus_tag="ACG8V1_000310"
     CDS             37478..39838
                     /locus_tag="ACG8V1_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0328435.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            39925..40239
                     /gene="trxA"
                     /locus_tag="ACG8V1_000311"
     CDS             39925..40239
                     /gene="trxA"
                     /locus_tag="ACG8V1_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0328436.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            40421..42199
                     /gene="uvrC"
                     /locus_tag="ACG8V1_000312"
     CDS             40421..42199
                     /gene="uvrC"
                     /locus_tag="ACG8V1_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0328437.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            42344..43243
                     /locus_tag="ACG8V1_000313"
     CDS             42344..43243
                     /locus_tag="ACG8V1_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328438.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            43236..44498
                     /locus_tag="ACG8V1_000314"
     CDS             43236..44498
                     /locus_tag="ACG8V1_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328439.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            44659..46557
                     /locus_tag="ACG8V1_000315"
     CDS             44659..46557
                     /locus_tag="ACG8V1_000315"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0328440.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(46887..47606)
                     /locus_tag="ACG8V1_000316"
     CDS             complement(46887..47606)
                     /locus_tag="ACG8V1_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0328441.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    47796..47843
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            47943..48275
                     /locus_tag="ACG8V1_000317"
     CDS             47943..48275
                     /locus_tag="ACG8V1_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328442.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            48262..50265
                     /locus_tag="ACG8V1_000318"
     CDS             48262..50265
                     /locus_tag="ACG8V1_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0328443.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            50284..50754
                     /locus_tag="ACG8V1_000319"
     CDS             50284..50754
                     /locus_tag="ACG8V1_000319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0328444.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            50775..51359
                     /locus_tag="ACG8V1_000320"
     CDS             50775..51359
                     /locus_tag="ACG8V1_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0328445.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            51374..52360
                     /locus_tag="ACG8V1_000321"
     CDS             51374..52360
                     /locus_tag="ACG8V1_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0328446.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            52350..52661
                     /locus_tag="ACG8V1_000322"
     CDS             52350..52661
                     /locus_tag="ACG8V1_000322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0328447.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            52683..54464
                     /locus_tag="ACG8V1_000323"
     CDS             52683..54464
                     /locus_tag="ACG8V1_000323"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0328448.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            54457..55833
                     /locus_tag="ACG8V1_000324"
     CDS             54457..55833
                     /locus_tag="ACG8V1_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0328449.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            55840..56475
                     /locus_tag="ACG8V1_000325"
     CDS             55840..56475
                     /locus_tag="ACG8V1_000325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0328450.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            56505..57860
                     /locus_tag="ACG8V1_000326"
     CDS             56505..57860
                     /locus_tag="ACG8V1_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0328451.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(58052..59446)
                     /locus_tag="ACG8V1_000327"
     CDS             complement(58052..59446)
                     /locus_tag="ACG8V1_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0328452.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            59538..60509
                     /locus_tag="ACG8V1_000328"
     CDS             59538..60509
                     /locus_tag="ACG8V1_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0328453.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            60506..61288
                     /locus_tag="ACG8V1_000329"
     CDS             60506..61288
                     /locus_tag="ACG8V1_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0328454.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(61457..61828)
                     /locus_tag="ACG8V1_000330"
     CDS             complement(61457..61828)
                     /locus_tag="ACG8V1_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0328455.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            61962..62405
                     /locus_tag="ACG8V1_000331"
     CDS             61962..62405
                     /locus_tag="ACG8V1_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0328456.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(62448..63257)
                     /gene="yidA"
                     /locus_tag="ACG8V1_000332"
     CDS             complement(62448..63257)
                     /gene="yidA"
                     /locus_tag="ACG8V1_000332"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0328457.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(63276..64643)
                     /locus_tag="ACG8V1_000333"
     CDS             complement(63276..64643)
                     /locus_tag="ACG8V1_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0328458.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(64627..65457)
                     /locus_tag="ACG8V1_000334"
     CDS             complement(64627..65457)
                     /locus_tag="ACG8V1_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0328459.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            65620..66057
                     /locus_tag="ACG8V1_000335"
     CDS             65620..66057
                     /locus_tag="ACG8V1_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0328460.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            66133..66753
                     /gene="rpoE"
                     /locus_tag="ACG8V1_000336"
     CDS             66133..66753
                     /gene="rpoE"
                     /locus_tag="ACG8V1_000336"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0328461.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            66942..67448
                     /locus_tag="ACG8V1_000337"
     CDS             66942..67448
                     /locus_tag="ACG8V1_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0328462.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            67465..68364
                     /locus_tag="ACG8V1_000338"
     CDS             67465..68364
                     /locus_tag="ACG8V1_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0328463.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            68451..69335
                     /locus_tag="ACG8V1_000339"
     CDS             68451..69335
                     /locus_tag="ACG8V1_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0328464.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(69527..69598)
                     /locus_tag="ACG8V1_000340"
     tRNA            complement(69527..69598)
                     /locus_tag="ACG8V1_000340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69563..69565),aa:Arg,seq:ccg)
     gene            69658..70992
                     /gene="rpoN"
                     /locus_tag="ACG8V1_000341"
     CDS             69658..70992
                     /gene="rpoN"
                     /locus_tag="ACG8V1_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0328465.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            71141..72232
                     /locus_tag="ACG8V1_000342"
     CDS             71141..72232
                     /locus_tag="ACG8V1_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0328466.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            72273..73274
                     /gene="gap"
                     /locus_tag="ACG8V1_000343"
     CDS             72273..73274
                     /gene="gap"
                     /locus_tag="ACG8V1_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0328467.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT        23441 a        13676 c        14103 g        22149 t
ORIGIN      
        1 gaattcattc cccaaaggcc ttttaagatt gatacagcag tccgttcact tcttttagac
       61 ttgtcataca agtcttttaa atgatcccat atcagactag tttgtctatc tagcttcaat
      121 ccattaaacg ctaatgaacg gttcattttt tctattactt tagataaagc tacagaaatt
      181 tttatacctt cacaacgcgc atcttggatt tcaataatag tatcatcaat tagtttttga
      241 atttcttgcc tatcaacata ttcaatctta gaaagagaat cttttaaagc tactaatgat
      301 tcaacgattt cttttttctc ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc
      361 atgtagagca gagtccattg aaccagtaat tacactatct acaacgccat tccaatttac
      421 tctacatttt ccatttttca gatttgtcgg tccatacgga tagccttttt gacattcaat
      481 gttcggatta taccagatag gattatacca ttcagccgca gaagcagatt cagtcgatac
      541 aaacaatccc acaccaacaa atccaataca catcacacta tagccaatta gttttttcat
      601 ctcgtatcca tctcctaaaa aatagaatag ttcttatagt tattcataag acatattttt
      661 tccgtttttg ttttatcctt tctgtcctat aacaactttt ttcaagtaaa gaatgacata
      721 gtttttctta aaaaaacagt ctgttttttt ctatttcttc ggtgtataat aaatatgagt
      781 gttcattttt tattttaata cgcactccgt acataataaa tcttgatcgc atcaatcttt
      841 tgagcttctt gatctacttt caattgaagt aatacaaatt gagtaacctt attcattgtt
      901 tcttctccaa ataacaaata ttgatccgga ttctctatat cttgtgtcac ctggtaaagt
      961 ttcccagttg ttttcatttg tacatgcggg tctacatcaa tactattgtc tttaatacaa
     1021 cgatctgtca tatagtccct cacagcttga ttgcgtttat aaacatctgt gaaattactc
     1081 cattttctag caaaatcttt taagaaattt tttgtcattt catctatttg catttgttgc
     1141 gctgtctcgt cttctaatga accaatttga ctgttacttt cagtactttg aagagttcca
     1201 cttgtttctt ttactgcttc gattgtttct tctgtcgttc tcaattgtgt tttttgtttt
     1261 tcagaagaaa accaatgaca accagtaaga cctatcaaac ataaccaact aaatactaaa
     1321 attatccctt ttttcacgtt tctagtcact gcctttctta tttcacctta cgaaccagac
     1381 tacgaatttt tgtttgattg tacgtgcgtt gataccgagc cataatccgt ccttgctcgg
     1441 cattctgttc cacactagag aaatgttgtc cgccatatga aacatctgtt ataattccgg
     1501 tgtgaccata gattccagga gacaatgcac cacctgcttg ccaattcacc acatctcctg
     1561 cttgtaaatc acttggtttt gggttcatga ttacttgcca accataggtt gtccagttat
     1621 aatcttcccc tattaacatc gcaaacatgt gtccagaacc cattaactgt gggccaccta
     1681 attgagatac ataccatgcc gtaccaccat aacattcccc attatttacg gatcgtccaa
     1741 tgatccgatc caaaaccttc gcattgcctt tactttttcc tgcaataagc gaagtttttt
     1801 ttgttggtag gctattccaa tgcttgatcg caaaagtatc atattccgtt aagttatagg
     1861 acgttatgat actcattgct cctcgataat acggactccc ttcggtaata cctgctgccg
     1921 catagcctcc acgacctaga atcaataaac aacttccagg atctttattg cccacacatt
     1981 gattaaagtt gctttggctc gccaccataa agccataata gtcataacaa gcttttaagt
     2041 tcggaaactt catatagaaa ccaccttcag aaccaccaat tccacgtgca gaaccttgtg
     2101 ggtagggaca accaggaaac caggtaatcc caaaaaagtt cgtatcgttt cttccagatt
     2161 gtgactgtcc ataagctgat tcatagccta attgagaaag aatcacactc ggtaataact
     2221 gatactttac agctgcacca actgctgctt gcgcatgttc atctgtgatt ggaccacgtc
     2281 ctttaatctc tgaaaaaatc ggtaatcctt cggtatattc cccattaaaa gcgacagcta
     2341 gacctgtagc ttctcctcca tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa
     2401 aaaagggaag aataaagaaa agaagacaac ctagacaccc tttcttcatt tcttcctcat
     2461 cctttcttcg ataatggttt ttggactgtt cgtccttttg gtaaagtaaa atcagttcct
     2521 ggttttgcgt ccaaaatcgg cagtgtttta ggatcctttt ctattttttc cgattgctct
     2581 gttggcttat ctaaagaaga aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt
     2641 ttactttcag acgaacgttg ccccggttgt tgcacagaac gatttcctga tagagtggct
     2701 accgacgaat taggaacaga gtattggttc aataactgct gcattgctgg ataactccca
     2761 tttttcgcct gatttttcac ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat
     2821 tgtggcacgg gtacttttcc agaaggcaac ggaatcgatt gaccaacaaa agcgatacct
     2881 ttatttaaca atggttttct ttgattattc ccttgttgct tactcaattg tgccaatgat
     2941 tgtacctgtt gttttggtct tactgaattc atggacgaat ttggaatcaa accatttgaa
     3001 agaccagaat tccactctgt tttcgatttg gtagaagaca tttcaggctc taccccatga
     3061 taacgaacat gacgaccact ataaggcaag gcttgatttg aacgcccacc tagttcaaag
     3121 ggattaacca cttgcctccc ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt
     3181 tgaaatccat gtatcgctgc agaagcgagt ggattcgttg ctcgatacac attccctgct
     3241 aatcctgcac ctttgattgc caaattttgt ccagcagttg atagttgacc aggcagttgt
     3301 ttcatttgag acaaagaatg acttgctatg tttttcgtgt tgtttatagt ttgatctgca
     3361 gctcctaacc ctagtttctt taataaatct ttccgtttta caatgagtaa aattgatagg
     3421 agtgctttga caaacatatt tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg
     3481 tctactatgt tatagatcaa aaatgccacc aaaataatca cactgtaaaa agatttttga
     3541 aacatcaagc ccaaaaagga ctttaaggta ttcatgagca tataatcaaa tactggaaca
     3601 aaagaagcga gtaatgaaaa aggaatcaca agtaataaac acaacaccaa catttgaaag
     3661 ataaaacgaa tcatgccaat cactacaagt ggtaatccaa tacttagatt aagcaatggc
     3721 accatagaac caacgactgc tttataaaca gctttagttg gttgaatata gacacgataa
     3781 gccttctttt tccctctatc ttctcctgca gcagtggtca cttttgtctc aatatcagat
     3841 aattcttcac ttgacgcttc cttggctaga aattcttgtg gttcaatctc tgcttgatac
     3901 aattcttcta aatttgttgt cccataattt aacaatatat acggatcaac aacagccgct
     3961 ttataataaa gttggcgcac tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca
     4021 ccattgacat cttttgtcgc caattcatct agactatttg tagcagaaaa aataaagcct
     4081 tctacctcca cggacaaatc atttaaccct ttggtaaaag cttctccttt actaggtacg
     4141 ttactaccat agccaaacca aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac
     4201 accatttttg cgtaatgttt ccctctaaat atacgaagag taaatagata taacacatac
     4261 aagacgacaa tccccgcacc aataggatcg tatatttttg aataaatagt atccgaaaaa
     4321 gtgaaaaact ggtgaattaa ctcgttcatt gcagctcctt catagagctt ctcaatgaca
     4381 aaatcaaggc cttgccaaat caatttactt aaactaaaga acatattcgt catcattttt
     4441 agaaaatctg tgccatttgc cccaaataac attttgccta atgaaaattc actatcagat
     4501 aaataagaag aaaaggtatc aatatgaaat ttcccaatct catagatcga ttgatccacc
     4561 tgcttttctg ccgcatacac caatccacca acaaaaagca gtccgaatcc aatcgttaaa
     4621 aaaaacaaaa cgcaccattt cttttcctta tgctttatat aagccattgt ttcacctcat
     4681 ttctattgca tgattaacgt atcctgttct tctacttgag agtgacttgt tttttccgtt
     4741 gtttgtaatg cagcggtcat ttcttcaaac aaactgtgta cattgagttt tcctacacgt
     4801 ccatagggat ctaacatcca acactgtcct ttaatcatgt ggctcatttc agctaagttc
     4861 gcttcattta cttccaaacg taaatgattc aaaatctttt ctcgctcact gtcttcatca
     4921 aaagcaaaga cgacaccaaa attccctttt tctttatctg aacctaagtc accaataaat
     4981 tgtgtatcta acactaattg attcatctgg gaacgcccca cacgtgccat tgctgcaata
     5041 atactacgtc ctgcgttcga attattaaat acccatgctt ctgtaaaata aatcgctgtt
     5101 ttcttactac tatctcgttt tccaaacatt tcacaaaaac gacctaaact aatcatcaaa
     5161 cacaaagact ttcgatccgc atctgtatat agttctggtc ggacattgtc atttggtaat
     5221 ttcaaatctt ggatctctaa aatcgttgtt ttggcattaa agtctaatcc cgggttttct
     5281 ccataagaaa atcctaaacg taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca
     5341 tattctcgaa cctctttttg ttccatttgt tctaaatagt cgaccacatg catcaagcca
     5401 acttgttgtc cggcttcttt ttgaactaaa acttcttgga ttccctttaa taccgcccct
     5461 tttaaaaggt gattggtacc taacggactt aactcatcaa tcatatcctg ggctacttgt
     5521 ttcgcatcta ctcgggataa acaaacaatt ggatctaata caccataatt acggggattt
     5581 gtagcatcta acgtgacata actcattttt tgcaaatgtt ctacaaacaa agggtaatgt
     5641 ttctgataat acgggtcttg aatgagtttc gtaaaccatt tgcgcttttc tcctttggga
     5701 tccacaaata aactttgtac attcataaac gaactataca aaaagagcaa gccaactaaa
     5761 aaggtcttcc ctttaccggt ttttccagtt actgctacat gaggcgcatc aaacaacgca
     5821 cctttgattc cttccgcaat ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa
     5881 attaattctt ctaatggtgt tttttctcct cgagaaccac catcccctaa tgtatcttgc
     5941 cgaccaatat accagccgga ttccatacct aattctgata ctgctgaaaa caaccattcg
     6001 ccaactgctt ctacagttgt tcggtgcttc caatgtaact gccgtcccaa tggttgccct
     6061 aataaacatt tatagaacaa ttctcgttga tcattttttg cacggtggat atggataatc
     6121 gtatgaagtt gttgaataac cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg
     6181 tatacaataa aacatgcgat ccaatcgacg acaggttgcc cattatctac tcgttgcttt
     6241 aaatgtttta ctaatcctcg aacagttaat aagcgttcac taggttcttc atcattttga
     6301 accaattcct tatgctctgt tttgaaccgt tccgatagcc aattcagttt taaaccaata
     6361 cctccctgtc ctttcaagcg tggatagtga attttcattc gcaattctgc tgggaaagaa
     6421 agatcctgag caaattgaaa tagatcacaa tttctcatat tttcaggtgt ttccccaata
     6481 ggcaacaatg ctaaataact actccctaac tcatcttcta attgaagaag gcctttatgt
     6541 tccttcggta tacgtacata cgttccttga ttgtctttca gtacttccca attattttgt
     6601 tcttctactt tgtgagccat tcctcgaata tattgcagac gattgagata caacagctct
     6661 ttttttacta agggacgaat agaaaagcta taaagtaatt gtgtcacttc ttttacataa
     6721 ggatcacttt cagaaaacaa agtttccaaa tcatctactt ctaatccaaa taagcgtgct
     6781 aaacttcctg ctaaatctaa ccagattaga tttattacat catgaaaact ctccaccgtt
     6841 gctttttctt tgactttgat tcctaaaata aatgaaggac gatagacttg accttctcgt
     6901 tccaacaatg tcactgtttc ttttgcataa tacgcaccaa ttgcttgact atcgggagaa
     6961 aatccctttc ctaatgtgtc aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc
     7021 aactccacat cttcataatc tgctaaaagc aacagtaaac gagccatcat ttcttttatt
     7081 tcctgtgtct tttttggtgt gttttttgaa attgttcgtg gaaaaacatg atagtaggcc
     7141 cagatacaat cctctccatt taacaaacaa tttttcttga atgcttttac tggttctttt
     7201 acaagcacca atgaatttca cctcccaata aaaaaggaac aaccagttga ttgtcccttt
     7261 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     7321 gataccttgt cccagttata atacacacaa tcaccattca tcatacgaat catataacaa
     7381 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     7441 aacacacata aataggggag tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     7501 atgacccaac caataaatga acgtaaaaat aaatattcaa agattacgac caacaaaaaa
     7561 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     7621 acgcgataag gttccgcata aacaatcgaa taatcataag gttcccaatc cacttcacta
     7681 tcctcctaat ccataaataa tttttcaatg attttaccaa acgcatcaaa tacacgtgtt
     7741 ggatcattta agaagaaata aactgcggca cctgctgcga tagatattaa tgcatttcct
     7801 acttttgatt tcataaaatt tcgcaatccc agataaacaa ccaccattat taaaaaatag
     7861 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     7921 tcctatccaa ccaattatgt gttaattttt caacataata ctgatttgtt tttttcgtaa
     7981 tgacaatcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     8041 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     8101 gatcaacttt agctgtttga ttgagtcctt caggttcttt catcatgtat gacatatctt
     8161 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acatctttcg
     8221 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     8281 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     8341 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     8401 ctggattcat cgagcgaata tcataagtta ctttataact agcagttgga atggtttcaa
     8461 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     8521 gttcttgaat aaagttttgt gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     8581 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     8641 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     8701 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     8761 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     8821 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     8881 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     8941 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9001 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
     9061 gtgcaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
     9121 tgatttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
     9181 tcccttcact atcttggatt aatggaatac catcttttac accaacaatt gtttgatcac
     9241 ctgctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
     9301 tgacaatcgg tacactcatt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
     9361 ccgatactgt gacttctttt gctggaacaa taggtttatt ttcttttgga attgcttctg
     9421 atttttcaac aggttgtttc ggttcttctt tttgttcttc actggtagac gacatcgttg
     9481 gctgttccgt acttgacgaa tcagtattct tttcagaaga actggttgtt cctgattgtt
     9541 ccggagttgt gactatactt gaatccgttg ttgttgaact atgcacacct tcttcttcga
     9601 tcgaatcagt cggtaaagtt gattcaggaa actctggttc tggtgtcact ggctcttttt
     9661 caggtttctc tgactgcaat tgttcaggtt ctgctggttt ttccgttgtt cctccattta
     9721 gctctgtctc aacatctggt aatccttccc ctggcaatgt ttctacaatt tctgacttag
     9781 gtgtttctac agtttcatct gcataaacca atgaactccc taattgaaac gaaaataatg
     9841 aaattcctag tgttgttacg actgctaatg ttgttttttt catgattatt cctccaattt
     9901 tttatgcaat tcgtgttttg ttcatagacg ccgcactaat attatctgaa cgataaaaga
     9961 actcccgtcc gttttcttct aacccaacat atggattatt tttgaataat tgaatcaata
    10021 gttcactggt gttgtatttt ggatagtttg caaaaaaata atactgtgct tgtcgcccat
    10081 ccgaaaattc aacaagtaat tcataacgat accgcttcaa tccttttcgt tgttttaaag
    10141 gatctctata ccaacttgtc catcgttcca ttcgtggata gagatagaca cgatacacat
    10201 agaacaatgc cataccacat accaccaaaa ataataatcg ccacataaaa attccccctt
    10261 ttcttctacc tttcttgaat catcgaacat tcataccctc tatagagatt tgaaaaatcc
    10321 atcatcaatt gttcatataa acgttgttgc aaatagcgat tgtttcgaat acataattct
    10381 tcatagccat caatcacttg atcttctttt tcatagcatc ttgtagctct atgacgctca
    10441 gcttgcatga cataaccaaa ctctgcacca tatcgttcta ctgttaataa ctgttgacta
    10501 cgcaataatg caatataaca atcacaaaaa tgttcaaccg acatttgatt tttctcacgt
    10561 ttgtgaaata ggtattcttt agaaagcaca ttattcgtta aacaatctga taaatagcga
    10621 taatctggcg gtcggttatt ttcatctgtg aatttttccc ataataaact gatttttatt
    10681 cgttcagcgg ctagatagtc ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt
    10741 ttatcacctg aataaacaaa tccatataat gcgaaacgaa ctcttccttt tcaattggct
    10801 ctcctaaaaa ttccagtgac ataccatctt ctccttccta aaaaaaataa aaaagcagaa
    10861 accgatttgg cttctacttt tctttccttt tatagctctt ttttacggaa gaatccgcaa
    10921 atgacatttt ttcaaaaaat caatcggcac aagttcacga aaacttggag caattttgga
    10981 tagaatcact tccccatctt gtaaaataca atcataaaaa tcatcagtta cttgatactt
    11041 acgcttctta caataattga taaaatagta gcgatcattt tctatttcca ttcgttgttc
    11101 tactaagcgc atacgaacat catcatagaa ataagccaca aatcgccctt caagttcatg
    11161 tttttcaatg ggatactctt ctggtaacaa tctatttttt cgtcgtttat acattgctgg
    11221 ataccttaga agaagttcat ctaaacgtct gtcagtatat ggtaactgac gaatcaacga
    11281 ttctaatcgt tctttataca gatagaataa ttggcgcaag caatagtgta ccgcctctgg
    11341 attttctaat actgttggat cacgtgacca atacgcacca acataaatca taattttttt
    11401 catttagaca atcacaccag caatactaat gaccgatcca caataggtag aacaagtggc
    11461 atagcgacca atttcctcta attctatctc aatctcttct aaagaaagat atgtctgttc
    11521 ttctgttggc tggcgactca tttcccaagt gacagtcacc aatgaaattt cttccataag
    11581 aggtttttcg ctgctaagtg ctgccacaca gttttcatat atcttcattt tatcgcagcc
    11641 ttttttaacc gctaaaatcg caggtattgg caatgaaaat cgataaaagc gaatgaattc
    11701 tagccttcct ttccttcggc taaattccaa ctcttcgagt tcttgttcgt atcccttacg
    11761 aacttctaat aagcgtgcaa tttcttgcca gttctctttt tcctgataac gtccagcttt
    11821 taccgcttgt tcaatataag gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc
    11881 atttaatgct tcgattgcag cctttaaatc caacaggcgc agccattctg ttgtcgtata
    11941 tgcattcatg caattttccc tcctgacgtt cccatccatt ctagatagtt ttgttgctcg
    12001 tcatttagtt cggcattatc aatcccttct tgtacaaagt ctcttcctaa aatacgatct
    12061 gcttcttgta acaactttaa tgtcggagct acttgtttca aaaaccagcg ttctgaacga
    12121 tctaatgaga atttttctgg ctttactgta aaatccaatt cttccatcga acgaaataat
    12181 tttgcccatc gtttatcaac aatccgtgca tcactttcca gtctatccca aacaataagt
    12241 tttgcattaa ttaaaccaac gccaatctct cctaaacgtt tcggtgaatc acctaaaata
    12301 aatgcttcta ctgcttgtgt tgctttctca ttggctaacc gtaactcata tcgattataa
    12361 ataccatatt tcgttaatgc ttctaattct gaaatttttt ctcggttcgc aatttcaaaa
    12421 cgtttctcat aaaaattaaa aaagagagga ctctttcgcg atccaaaata caaagataat
    12481 ccttcacttc gaaaatagcc atcatctaaa aaacaaccgc caatattctg gaatctctta
    12541 aagtccattt ctaataaacc tgctttttgt ttttctagta attcaaagag atcgaaatgt
    12601 tcttccgttt ccaatcccca aagttcatct aacgcaatat cgaatcggct acattgcaca
    12661 cgtgtataac cagcttccaa ctttccttga tttttccaaa tggaatatag ccatgttcgc
    12721 caatctgttt gttgttcacg catgacttcc tcatattccc ggcaaccttg tcctgaaaat
    12781 tgcacaagta cattttctgt tacagttcgt tttgaatgcc agaacaccca gatattccca
    12841 cgaacagaaa gacagtcaaa aagatacaag cccgttggac gtgtttcaaa ttctgtcaga
    12901 tccattcgta atacacgttg aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc
    12961 aaataatcaa tcattgctcg aagatctatt ccgcttttca tatacaatcg aaagcgttcc
    13021 ttgattcgtt gttcaaaatc ctctgggatt tttcttttcc cttgttccat ctttctaatg
    13081 taatcttttg agcagcccat caattgcgca aatcttgctt gtgatagcgg ctttttatct
    13141 ccacttatat tatgaattgc acttaacttc tttctgtagc tttttagctc aatcggtgtc
    13201 attttctcgc tccttttata aaataacaaa atcgattttt tttaaccctt tagaaacacc
    13261 gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct gaccttagat
    13321 aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt atttctgccc
    13381 cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc acgacctctt
    13441 tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg cagccttaat
    13501 gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc gatagataat
    13561 aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg agaaaaaacg
    13621 cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata caatcgagtc
    13681 aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc tgtaaaaaca
    13741 cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa ccaatcataa
    13801 aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac tctataataa
    13861 caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg catcagaaaa
    13921 acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt cttgtaacaa
    13981 ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa aatcatagac
    14041 aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt aaacaaatgg
    14101 cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac caggttgatc
    14161 tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg
    14221 agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac ttggaaaata
    14281 ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac gaccctttaa
    14341 tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca acgaaccata
    14401 aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat aagcataatt
    14461 tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc gttgattcat
    14521 ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt aaaacacatg
    14581 tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc catcgcaaat
    14641 atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat aggttttccc
    14701 accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc aatccaaagt
    14761 tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa tccgaccaga
    14821 atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg
    14881 caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc
    14941 atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa cttttggtaa
    15001 tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa caaaccgatt
    15061 gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat atccttcttt
    15121 tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca aatacaacac
    15181 aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta aacgtggata
    15241 aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa agaaaccata
    15301 atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat acttgaaaaa
    15361 atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt
    15421 ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata atcgaaaaag
    15481 atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta tacgaatcca
    15541 aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa caagatcacg
    15601 taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta caaaaatcgt
    15661 tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag cacaatctct
    15721 caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta tcttagataa
    15781 aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc tctttttcct
    15841 tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt ttcatattag
    15901 gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact aaatctgaat
    15961 attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga tcaattaatt
    16021 tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat ttaactcgtc
    16081 gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt gatcgaattt
    16141 ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct aatgcgccaa
    16201 agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa ttatttggaa
    16261 attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa gacaccaaca
    16321 tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc acgactattc
    16381 actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac ctctttttct
    16441 ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc aggctgttct
    16501 tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt aatttccttt
    16561 ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc tttcttcata
    16621 cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa
    16681 tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt cctcatgact
    16741 aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact aatactacta
    16801 atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac attggtacaa
    16861 cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt ggcgcttcca
    16921 caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa
    16981 acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc aagccttttc
    17041 ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt tctttatcgg
    17101 ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt gttaagtagc
    17161 cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg acccattcaa
    17221 gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc gtcgctttcg
    17281 tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata tctgcgtgag
    17341 aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct ttgcctcgca
    17401 acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt tctgccacaa
    17461 acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc gttactttgt
    17521 ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg tatttcacag
    17581 cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg
    17641 tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt
    17701 ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg acgatttcac
    17761 gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt
    17821 tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag ggttcaccgg
    17881 ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga atctcggtaa
    17941 tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt
    18001 caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg gcttcatcgt
    18061 tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca aaaacaacga
    18121 tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt gtgccattga
    18181 ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt aaaattggcg
    18241 cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg acaaacaagt
    18301 tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc actttttcac
    18361 catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag
    18421 aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc gtgaatgctt
    18481 gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag taatagtcat
    18541 cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat tcaccaattg
    18601 cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt
    18661 tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc gcaaaatgga
    18721 tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa
    18781 cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca taagtaagtg
    18841 taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat
    18901 catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg
    18961 tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt
    19021 gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga
    19081 cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtacg
    19141 tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct gtcacaacgg
    19201 ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca gctaattttg
    19261 gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc actaaatcaa
    19321 tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca ggttcaacaa
    19381 caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt tcttttaaaa
    19441 catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta atttttttca
    19501 aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc gcattttcta
    19561 aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac acacgaatgt
    19621 ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt ggatgtaaca
    19681 caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga actttaattt
    19741 tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc catacttcat
    19801 tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca tatttcaaaa
    19861 aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt gtttgattat
    19921 taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct tttcgacgat
    19981 cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa
    20041 attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag
    20101 agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc aaagcagtag
    20161 ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc
    20221 cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc ggactagaag
    20281 taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct gcattggttg
    20341 cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg
    20401 tttttggaat gttctgctct tttttctttt gattttcaat actagccgta gattctttgt
    20461 ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt
    20521 cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc
    20581 gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc gttgcttcgg
    20641 cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt
    20701 cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct
    20761 gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag
    20821 tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga
    20881 cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag
    20941 cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat
    21001 tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa
    21061 acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc
    21121 gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa
    21181 aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttttt
    21241 ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat ccttaatttc
    21301 tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac tattgccaat
    21361 tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac cataatgatc
    21421 cgattcctca gtattattgt cattactagc tactggtttg gttgttctac tggcttattt
    21481 tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc ccattcaaac
    21541 cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct
    21601 tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc
    21661 catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc
    21721 tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct
    21781 caaataactc tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta
    21841 acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt
    21901 tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt
    21961 cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc
    22021 acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt
    22081 attaaacact aaaactttcc gcattttttg ctcattaatt aatcacgcgc gaaatctagg
    22141 aatcacaact tattcgcttc ttctttataa atcgccgatt gtaaaattaa gctagacaac
    22201 taaaaaaagt catttgtgct acactcaaaa tagttccgac gaaagaatta taaggagtga
    22261 gtacaaatga cctataacca tcttacacca actgaactcg taatgataga agcttatttc
    22321 aatcaatcac aacctgtttc aaaagtagcc aatctgttac aacgttctcg acaaactatt
    22381 tataaggtct atcgttttct taaatctggt ggaagcgcaa tgagctacta taaacaatat
    22441 aagaaaaaca aacgtaactg cggaagacat ccgattgttt tacctgatga gcaacaggaa
    22501 tacattcaaa aaaaggttgt tcaagggtgg gctcctgatg tccttattgg tcgagcagag
    22561 tttcctattg cttgttcggt acgcacactg tatcgaatgt ttaaagataa aacctttgac
    22621 actcacgatt taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga aaaaagagga
    22681 aaacaagggt ttcgccggtc aatccgagac agaaagacag actataccca gtttgatcat
    22741 gaatttggtc acctggaagg tgacactatc gtgggtggaa aacataaaag cgcggtcatc
    22801 acattagttg aacggctatc taaagtcatc attaccttga aacccgaagg cagacaggct
    22861 aaagacattg aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa cttatttaaa
    22921 tccatcacat ttgattgtgg aaaagaattc tcaaattgga aaaagattag taataccaac
    22981 gatatttcaa tttattttgc ggatccagga acaccttctc aacgaggatt gaatgaacat
    23041 tcaaatggct tattacgtaa agatggtttg tataaagcta tggattttaa tggtgtatct
    23101 gaaaccttta tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa atcattacac
    23161 tatcggacac caatggaagt atttttgagt tacgtcacaa aagatgattt gtctaactta
    23221 atttgacaaa cgaaacttaa taaaaaagga agaatattca tgtaaacgat tttattgttt
    23281 ttttctcttt tatacaaaaa gggtcataat ttcaagtaag cggatgaatt catgaggatt
    23341 ttttgcatac tgtctctact atacttaggt cactaagaaa aaggagtgat ttattatgaa
    23401 aaagaacatg attgggacat tattactttc aacacttctt ctaggcggct tggcaacacc
    23461 agcttttgct gagggtcaag cgacttcaaa aggagatatt acctttactg aaccaacaaa
    23521 taccgtggag cctcttaacc ctactgatcc ttcaaaacca gtagagccag cagatccgga
    23581 aaatccagca actggccaga caggtccttt aacactggat gtagttcccg aattgccatt
    23641 tggtacacac gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt caaaaaacga
    23701 tacaccttat ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc aagcacaagg
    23761 gtggaacgtg acagtttcgg tttcagattt tgtaaacggt tcacaagttt tgcaaggcgc
    23821 tgaattagat tttggaacat ccactgttaa aagtacgtca gataacgaat ccactggacc
    23881 aactagtcaa actgtaactg ggttgagtaa agcaagtgca gcgacaccaa tatttacagc
    23941 agcaaaagat caaggtctgg gaacatggct ttctgtttat gatccagcaa atatcacact
    24001 gaaagtgccg aaagcagcag ctggaacatt cacggctgat ttgacttgga acctcgtggc
    24061 tggcccagta gcatagagta tgaaataaat tcaacaaact gcggtgaaaa tactataggt
    24121 gaaggatttt ttaccgcagt tgttgttgaa aggatgtggt ggacaatgaa tcaaagtaaa
    24181 cgactgtttg tttcgttttt ttctatttta agtctatttt tcatttttcc gtcaatcagc
    24241 caagcaaagg attcagcagg agactttgga atcaaaccgg tatttccaga gaaccaaata
    24301 gataaagcaa tcggttattt tgatttatta gtggcaccgg aacaaaatca gacactagaa
    24361 gtaattatca gcaattcttc agatgaagaa cgaacatttg aggtctctgt caatccggcg
    24421 gtaacgagtg atggtggaac gatcgattat agtcaaaaaa acccaacgtt agatgagaca
    24481 ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa tgtatcagca
    24541 catgcagaaa caactgtacc tatagaagtg aaaatcccag caaaaagctt taagggacgt
    24601 gtattagcgg gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa tgctacagaa
    24661 ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag tcgttttaca agaaagtcaa
    24721 gagacgatag aaccggatct gaaactttta tcaggagatt tggacgaggt caacgcaaag
    24781 ccaactgttc agcttcgttt ccaaaatcct cagcctagga ttatcagcaa tttgatattt
    24841 acgagcaaga tcttttatga aaatcagctt tatattgaaa atacgtctaa tgcattttta
    24901 gttgcaccaa attcgaattt tcacttgaat cttgatttag ctggtgataa agcaaaagcc
    24961 ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg gcgatttaca
    25021 caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc cgtttttgca
    25081 gtacaagaac aatctttccc atggctttat gcaggattgg ttgctgcagt tctagtgtta
    25141 tggttgattt tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga aacaacatga
    25201 tgaggtgatt tcgttgaaaa catcgatctt attgggaggg attattgcaa gtttgctgtt
    25261 cttcagctgg caagagccag tccaagcaga taccaaaagt tcaggtacga ttacatttta
    25321 tgatctaagt ccaattccgc atccaagtgg ttcagtcaat ggcgcatcaa aacagcagac
    25381 acagctaccg atttataaag aatctaatca acatggcgta ttcccggaga cgaatgaaca
    25441 aaagatccgt tttctttatt tgatcattgg tatagaatta tggcttcttg cgtggttgtt
    25501 gttatggaga agaaaggaaa aggaggatga acatgttgct acaaaataaa ttttcatttt
    25561 ttcgctcctt cttttttact tcgctttttg ttttaagtat atttatcttt tcaccttcgg
    25621 cagaagctgt tggagcaacg cctcctgcca tttcttatga tccatacacg tatgaaaata
    25681 tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc caactaactg
    25741 gggctaacca attgcctaaa ttgactgcta cttatttgaa cgatggttca aatgatcctt
    25801 ggaaaggtgg cacggtccgt tttcattggg aaagccatgt gaaactaaca aatgtacttg
    25861 atttggtgaa tatcactgca caaggatttg catttgtggc gatcatcaat ttgccggaac
    25921 atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa caacgcctac ttgtatattg
    25981 ccggtgcccc catcaaggtt actagcggaa gtttattatc agcaggaagc cataccttgc
    26041 gttttgggat ggggtcgtgg gatacaaata ctctattgac agatattatt gctattatta
    26101 cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt tgatgttaca gctaatgtcg
    26161 cgaagatgac agaaaacgga gataaatatg atacgagtcc aaacaaaatt ttgaccaagg
    26221 gtaaattaca accttctatg tctaaaaagg ccgttttttc tgttgatttt tatgattcga
    26281 ataatattat ccaagatcgt atgttcaatt cgattttagc tccagctgca ggtagatttt
    26341 atcctaaatt aactggcgga aacttgaatc caggagcata tgcattttct gcaagtgcag
    26401 agataaatac gtggaatagt tacatctcac caagtgattc aacaggaaaa tataatacat
    26461 ttgctgatac cacagaatac gtggatggaa ataaccagaa ccttccaggc tctaccaata
    26521 gtcggacatt gacgatgggg ctgactgaag gacgttttga tcagttgcca tttagtcgct
    26581 ttgatcgcgt agtcaactat tttacaggaa aaaacgttac acaaggagca atactgactc
    26641 atactccagc aagcacaagt ggattgaatc agacagccaa tatcgtgtac agtgggaaag
    26701 atgcgcaagg cggattgctt tctcctgttg ctttaaaggt attggagcag tacaagctag
    26761 acggaacggt tttaccaaca aacttaacag ccgatagtca gtggggaaag ctcctttctt
    26821 ctgcatcgta ccaagtcacg gccaattggt cagcaactga actaaaaaag ggttcgatcc
    26881 attacaggct ttattcgaaa ggaacccaaa aattagcgac aggatggaca gatcaacttt
    26941 tccaaacgat ccaatcaaat aacgggggtg taaatacagc tacttctaca ctacctgcat
    27001 tggcttcagg gcaatattat tttgattatc gcttggtgga cgatgtactt actaacatgt
    27061 atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg aatcaccact cttcctcaaa
    27121 taacagtggc taattttcca tctgtgagtg cagatagtca gctaaaaaac cttagcagac
    27181 aacctgagac aggagatccc ctgtctgctc tatcaggaga tacgattcaa gaaaatgtga
    27241 cattcacgtt aacaaaatct ggggatgcga taaccgacaa aaaaatcacg atctcacttc
    27301 ctgaaaatac tacttatatc aatggttcct taaaattaaa tggtacaact atcgcagata
    27361 ccggcattca acaaggagtt tctgtcccag cagatctatt gagcaaaata ggagatacca
    27421 ttcatcttac ttacaactat caattaaata ctgtagatac ttctttacaa tccgtcagca
    27481 tactgacaaa ggcagctgta ctttccagca atattacatt agcagatggt gctaagcttc
    27541 ctaacccagt tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa gaattaacat
    27601 tagttaatgt accagatgat tttacatttg gtaacgatct ccccaaaccg ttgaagacaa
    27661 gttattatga atcaaaaggc gatttttctt ttgatgtgcg ggatactcgg cttccaagca
    27721 cgtctccttg gcaactgaca ggtacattga cttctttatt taagaatgat caaggtcaag
    27781 aattaagcgg aacaaaatta tacttcaatc attctggatc aaagcaattg attcagcaag
    27841 gtcaaaacac gttgatttat gagagtgatg gaacagctaa aggagaggtt ctcgttgact
    27901 ttcccgatac cgatggttta ctcttagagg taaatagcag tacgaatgct caacctggcg
    27961 caacgtatca aggcatggtc acatgggaac tgacagctgg gccaaccagc taagtgatta
    28021 gaatttgtat ggatctgtga taactgtcac agatcctctt tttcaaggat gagaaggaaa
    28081 cttctgctgt taaaatcaga aatgcttgaa ggagtggact tggaactttt cagtttgctt
    28141 gaaaaaaatg aacaacggca aatcaaactt ttccaacttt tattagagga aaaaggagag
    28201 gtttccgtta ataacgtact tacccgctta tcagttgatc gggctacctt gaaagaagat
    28261 ctgttttatt tacaagctaa cttggaaaat atcaaacaat tggaaattgt tatccaagaa
    28321 gggaatattt tattaatcag acatggaaat atagcagcag tcgatgttta ttatcattgt
    28381 tacttgcgct attctacaaa atatcagata cttctttatc tgttggaaaa tggctcattt
    28441 gatcggcaga aacttgtcat ggaacttaat atcagttcgg ctactttgtc tcgcagagtg
    28501 aaggaattaa atgattcttt aaatgagttt catctgaaaa taaaaagcgg gcggctgacc
    28561 ggtccagaaa gtcaaattag ttatttttat ttccaattat tttggtttgg acgtccctat
    28621 cttgaaaaca aaaaagaata cagtgattta tcaggaaaca cttttttaga tctgctgcaa
    28681 aaagagttta gttttgctat tacagaagaa ggacgattga aatttgaaat atggctttcc
    28741 attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac cagattatag caatcttcct
    28801 gttggattgt ttcaaacgac acaattttta ttggacctcc atacattttt gacccgttac
    28861 ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt tggtttttta tcttttcatg
    28921 atcagtaact ttacattaga tacacataac tcgcatgtcc aggagttttt gataagcgaa
    28981 aaatacaaag acgacgtagt agctcaattg aatcgaacgt ttaaacaatc gatagaagaa
    29041 tatttctctt tttatgtttt tccagaagag atttcgcaaa agattgcgtt gacagtgatg
    29101 caaaatcacg ctcattttgc ctattttaga ggatggatga atatttttgg ccaagaagga
    29161 ttaaaacagc ggttaactga agtagaagat aaacaattcc tggttttatg tgaaagattg
    29221 gtcagacaaa catttgctat tgttgatttt actgaagtag acgaggaaaa tgcccgcttt
    29281 gaattatttg ctcgctatgc atctatttta cggacgctga tcaagcagtt agaattacgt
    29341 ctgcaaattg cttatgatct tccttatgaa aggctaatgg cggatatcgt gaaagaaatc
    29401 atccaggaac aattgctgaa tacgatcaaa tatgatttag tcgaatacga gaaagaaaag
    29461 cagtatgata tcattttgac tagccaattg aaagagtacc cgtcacagaa gtccgctaaa
    29521 gtttttgtat tcactagcaa cgaatataat tatgatttcc cctatttgaa ccaattttta
    29581 aaagaatgtt atttagaaaa attgaattta aggatgaata aaacatgaaa atcgtgatca
    29641 ttggtgcttc ccatgaaggc atcacagctg ccctaaatct aagaaaactt caaccgaaag
    29701 cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt tcaaatggaa
    29761 tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat aacatgcgga
    29821 ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa cttgatccag
    29881 acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata acctatgata
    29941 aattaatcgt agcgacaggt tcctctcctg tcaccttgta taaacaaatt gaagctgaaa
    30001 atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct ttaaaagaag
    30061 caaaagaagt agtgatcttc ggtgctggtt acattggatt ggaattagca gatgctttga
    30121 gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta tcaagatatt
    30181 tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa ataaattttt
    30241 atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta tccgtacaac
    30301 ttctgtctca aacaataaag gcagatatgg tcattttttc tgcccaaact cgtcctaata
    30361 cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata gtcaatgaat
    30421 atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca gtcagttttg
    30481 acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat atggcaagag
    30541 cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa aagataacag
    30601 cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat gaagcgccat
    30661 tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca caatacgacg
    30721 aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta gaaataattg
    30781 gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt gcggatatcg
    30841 tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt ctgtctgaat
    30901 acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct aagagagcaa
    30961 atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt gtgtggggat
    31021 ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc ggaagaattt
    31081 tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat aaaatgatta
    31141 tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt caaagttacg
    31201 ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt ttttgactgg
    31261 gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg aggcagattc
    31321 gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta ctaagaagat
    31381 agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc caaacacctg gagtcgtgac
    31441 tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta tcgataaaaa
    31501 tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg atgaggcagt
    31561 aggcgatatt agtttgaaag aagaatatgg tctagccata ggagatacag ttaagttatc
    31621 tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat tcaatgtctc
    31681 accagttctc tatacaacca tcaatgctta ccaagaaatc cgcttcgaaa aagaagatac
    31741 aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta atttacctga
    31801 agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat ataatgcgca
    31861 agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta ttgtgatcgg
    31921 gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa tgaaagcaca
    31981 agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca ttttatcatt
    32041 tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc tacctgatgc
    32101 ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga tgttagtagt
    32161 agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc cgttgaaggc
    32221 aattggataa gaaggaggaa gaacatgaaa gcaatagaat ttaaagaagt agaaaaaagt
    32281 ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag tatcgataaa
    32341 ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt tttgactttg
    32401 gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca agaattcagt
    32461 aatcaaaaag aaagcgaacg cgttaaacta cgttttaaag aaattggctt tattttgcaa
    32521 gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt agataagatt
    32581 aaacatgaac atcgtcaaaa agaagcaaac gaattatttg agcagcttgg cgtggataag
    32641 ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt cgcaatcgct
    32701 cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc tagtttggat
    32761 tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga caaaaataaa
    32821 gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag aatatttatg
    32881 atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa aaatggtcca
    32941 tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact tgtttatgtc
    33001 caagggatat ctcggcactt caacaagaga aatcgcacaa aaagttggga tcacccagcc
    33061 caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt tagaaaacca
    33121 cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa cgggatttca
    33181 agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg atttgtttct
    33241 gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc ttttctttct
    33301 atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc aatctgtgct
    33361 aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt tagcacctta
    33421 catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat tgatcgattt
    33481 atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa aatagttagg
    33541 tgaagaagca agatatgacg aagatcgtta tatcttgctt ctttatatag gtagtttttc
    33601 ataaatacaa atttgttatt ctctcatgat tatcagggta tatttttatt tattatgtcg
    33661 gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta gaatgaagga
    33721 aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc aggatatgcc
    33781 aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat cttttacagc
    33841 cccttttttg cttacttaag ctgattttaa gttttggccc ttagtctgga tctacgaggt
    33901 gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt atcattatta
    33961 tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg gtcagcagta
    34021 cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa gagacattaa
    34081 tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact accccacttg
    34141 caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat gcttatcaga
    34201 tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc aaagtcacaa
    34261 agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta gatcctgggc
    34321 atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa aaagatataa
    34381 cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca acggtttatt
    34441 tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac agtgaagaaa
    34501 aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg aatgaagcaa
    34561 ctggaatcac gacatattat tactatggac aagaggaaac attagcacag acaattgctg
    34621 actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac tatcaagttc
    34681 ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat aatgatagtg
    34741 atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt ttgacacaag
    34801 cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag ctcctcatat
    34861 ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt taaacaccgt ttaaggaaga
    34921 ggtcgtgagc ctgccgttca actctgagtc acaaggagca atttcaaaaa acagcttctc
    34981 atgtttgatg actattgtga cttgtgaccg aggagttggc atgagaacac cgtttaagaa
    35041 aaaggttgtg aaatcaacgt tttgttctga gtattagaga aacaaacgga aaaatagttc
    35101 ctcatatttt gccgtttgtt gagctaatac cgaggatcag atgattgaac accgtttatt
    35161 cggaacaagg gactgtgaca tgacttttgt cacagttcca ttattttttt agtagcttca
    35221 aagctttttc tgtattggca aaatgaagtt tggcatattt ttcaagcata ggcatgccgc
    35281 ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct gacggaaggg
    35341 tgattccagc ttcagctgat gctttgtcta actcttccaa aaagctggca cgactgatga
    35401 tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag tacagttttt
    35461 cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc ggcgtaaact
    35521 gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc aaatggatcg
    35581 cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg atctcattgt
    35641 atttttctgg agataagaca agcagtttat aggggatggt tttttttaag atgtcagcaa
    35701 gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt ttacgtaatt
    35761 ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggaccg aaatagctgc
    35821 catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt ggcaaagaag
    35881 aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta tcagcttcta
    35941 ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta atggtcgtcc
    36001 cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa taagagcgat
    36061 agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg ttgctcataa
    36121 aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc gttttctaag
    36181 agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt atgtgaaaaa
    36241 tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa ctttcatcaa
    36301 ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcgg tgattgccaa
    36361 ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg taacgaagct
    36421 gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata atgaacaagc
    36481 agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag aaagaatatt
    36541 ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta cagagcttga
    36601 aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat tgaaagaaaa
    36661 tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt taaacgagaa
    36721 acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca aaataagaaa
    36781 aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga ttttattttt
    36841 attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa tcatattcgc
    36901 gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat tagcaagtca
    36961 tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag cattttttga
    37021 tcaggtattt gcctttcacc tagatgaagc tttttatgca ggaactgctt ttctgattat
    37081 tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg gattgacgta
    37141 tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac taatcatggc
    37201 ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag atgtggtcca
    37261 aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac
    37321 caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa
    37381 aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc
    37441 acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac
    37501 attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa
    37561 agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca
    37621 agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaaat
    37681 tgaaaatatc cgtccccata tgaaacggat tgagatcgga gcagacttga atggagtgga
    37741 gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga
    37801 tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac
    37861 gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga
    37921 tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt
    37981 tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat
    38041 catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt
    38101 tggcggtgta gtccacgatc aaagtgcttc aggtcagact ttatttattg aaccaaaaca
    38161 agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca
    38221 acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa
    38281 tacttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt
    38341 aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca
    38401 tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca
    38461 agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg
    38521 tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat
    38581 cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct
    38641 atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa
    38701 aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct
    38761 agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca
    38821 ttatcctgaa ttgaaagttt atggatataa tcgggcaaat acgattaatg ccagcatgga
    38881 gtttgatgtg gatacattaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag
    38941 caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa
    39001 gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg
    39061 ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga
    39121 acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat
    39181 ggaaaaagca aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa aagcagaaaa
    39241 aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca
    39301 tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa
    39361 aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt
    39421 ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt
    39481 agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca
    39541 aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc
    39601 gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata
    39661 tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac
    39721 aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt
    39781 tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag
    39841 cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata ctagtcatag
    39901 attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa
    39961 gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt
    40021 atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt
    40081 gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt
    40141 ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca
    40201 aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc
    40261 tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt
    40321 caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca
    40381 tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa
    40441 caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac
    40501 gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg
    40561 tagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt
    40621 tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc
    40681 taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga
    40741 aaaatatccc cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac tgtattttgg
    40801 accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc
    40861 tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca
    40921 atgcctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga
    40981 agtgaaaaac tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat
    41041 ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg
    41101 tgccattgag acaatcatga cttgccaaaa aatgacaaat actgatttac tggatcgaga
    41161 tgtatttggt tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt tcgtaagaca
    41221 aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga
    41281 tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt
    41341 cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct
    41401 gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga atgcctcagt
    41461 tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt
    41521 tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa
    41581 ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc
    41641 ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc
    41701 atcaatgaga gaagtcatct atcgcagata ttcgcgagtg attcgcgaag ggttgccttt
    41761 acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt
    41821 agaaaatcaa ttaggaatcg atattcctat tgctggcatg gtaaaaaacg ataaacataa
    41881 aacaaatgag ttgctattcg gtacagattt ggctgtagta ccgcttgagc gaaattcacc
    41941 agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca
    42001 tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aagggttagg
    42061 accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc
    42121 cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga
    42181 taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt
    42241 ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg
    42301 gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga
    42361 tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga
    42421 tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt
    42481 gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg
    42541 ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt
    42601 aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat
    42661 tgaaccaaaa atcgactttt ggatggaaaa atttcaggta aaagggaaaa agacggataa
    42721 agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca
    42781 tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga
    42841 attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag
    42901 ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa
    42961 aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt
    43021 cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat
    43081 ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat
    43141 ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt
    43201 ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca
    43261 aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt
    43321 ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca
    43381 ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa
    43441 cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg
    43501 aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc
    43561 tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg
    43621 gcttgcaatc gatgcttcga gcgagtcaat taggcatttc accagaagaa gtagctagct
    43681 tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga
    43741 cagtcggaga agataactcg acagtccaat acgtcgtaag ttatgtagca acgattgtct
    43801 tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag
    43861 gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa
    43921 aattaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg
    43981 gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct
    44041 cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca
    44101 ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata
    44161 cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatacatg cttggattga
    44221 tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct
    44281 tatcttcata tattatgccg attcgcttag caaatgagac agtcggtatg ggtggagcat
    44341 tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca
    44401 tgtacaaatc gaatgtactc gtttatagcg aaggcggatt atggagttca ttgaaacaat
    44461 cgatttcaat tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa atacagaagg
    44521 aagctggaca tctattgttc ccagcttctt tttgtataga cctattttat ttattttact
    44581 atattggata tatattcatt gaaaatagtg tataacaact ttataatatt gagtatacca
    44641 atttttggag gcgttttaat ggaccgttat ttagatttac tttcagaaaa attcccctca
    44701 acggaggatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga
    44761 acagagttat ttttgagtga tatacatggc gaatttcctg cttttgatca tattctgcgt
    44821 atcggttcag gaaatcttaa agaaaagatc aaagaccttt tttctgaaca aatgacggaa
    44881 acggatatca gccaattcac tatttttacg gcttatccag aatatgcatt aacgactgat
    44941 tggtacaaag aacaagataa atcgcagctg atccatcatt tgatcgatct tttacggttt
    45001 acgacagtca aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga atatagctat
    45061 atcattgagg aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga ctatgtaaaa
    45121 aaaatcattg aacaacttgt tttgatgaag gaagaagaaa aatttttaac gaaacttgca
    45181 cagacgatcc aaaaatctgt catcgatcat ttgcatatcg ttggagatat ttttgaccga
    45241 ggaactcaag cagccaaagt gatggatcga atcatggatt tttcttctgt agatatccaa
    45301 tggggaaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc atgtttactg
    45361 acacttttac ggattgcagc acgctataat tatttatttg aattagagaa agaatatggg
    45421 ttgaatttac gtccattgtt ttcttttgct catcaaactt atcagaagaa tccagtattt
    45481 gcgcctaaaa acagtaaaaa tctatccgaa agagagcaag aagaattaga aaaaatccat
    45541 caggcacttt ctatccttca attcaaatca gaacatcagt tattggatcg tcgacctgaa
    45601 tttaaaatgg acgatcgtaa attgctggaa aaaatcgact atgaagcatc aacgattgat
    45661 ttagaaggtc agctctatga gttgaaagga acttgttttc aaacgatcca gccagatgat
    45721 ccaaaaaaat tcttggaaga ggaacaaaaa gtgattgata gtttgatgta ttcattccag
    45781 cactctttga gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg aatgtatctc
    45841 acgaacaatc agcatgtgct gtttcatggc tgtatacctg tagacgagaa gggacagctc
    45901 ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggctt ttttgaaaaa
    45961 cagataactg aaagcgcaaa agatctgact gcaaaagaag atttagcaac cgatttgatt
    46021 tggtatgcgt ggagtgggcc attttcgcct ttattcggca agagtaaaat ggcgactttt
    46081 gaacgttatt ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc ttattatcac
    46141 ttgcgagatg aagagtggtt cagtgaaaaa attttagcag aattcgacac aaaagaagaa
    46201 gggtcagcaa tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga gtcaccgatc
    46261 aaagcaaatg gaaaagtatt tgtcattgac ggaggtttat ccaaagctta tcaaaaaacg
    46321 acaggaattg ccggttattc actattatgc aattcttatg gtttccaaat cgtgacacat
    46381 tatccgtttt tgcctattga acaacaattt gcaaaaaaat ccgaccaaac aaatgtgaaa
    46441 aaagtaatcg aacagcaact accacgcaaa ctcaatcgtg atacaacaaa aggtatggaa
    46501 ctgcagcagc agattaccca gctcaaaagg ctgttggact atcgaaagga cgattgagaa
    46561 gaattccagt gaacattgtt ccatagactg gagactgaga caaaagtcgt gtcgcagtct
    46621 ctttttccgg ataaacggtg ttcttctgcc aactcctcgg tcacaagtca cactattcaa
    46681 caaacatgaa aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct
    46741 cacgacctct tggtataaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
    46801 tgcaaaaatg tgaaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacagct
    46861 ttttcacaac cccctcagaa agtctattaa aattcattca caaagaaatc gaataagcgc
    46921 tgttcagatg gatgtacacg gtgggtaagt tccggatgcc attgaacacc gaggatttta
    46981 tgcatatcat ctttgctttg aaccgcttca acgagtccat cgttagaaaa agcgatggct
    47041 tttaataaag gtgaaagatc tttgactgcc tgatgatggt aagaattcac aaaatatgga
    47101 tcggggagaa ggttgcttaa caaactgtct gaaacaatct tgacttcgtg agtcgtaaat
    47161 tgcggggcag tgggctgttg ttcatgtttt actgaccact ttggatataa tgaaagatct
    47221 tgataaagtg taccttccaa aacgacattc aatagttgga gtccccggca gactgcaaaa
    47281 attggttttc tttgtttcaa tgcttccaag atcagtgcct gttcaaattg gtctcgttga
    47341 atatttgttt ccgttagctt tggatgagga tcttccatgt aaagttgtgg actgacatct
    47401 tgaccacctg caagaataag tttgtcgatt tgtccaatca gttgaggagc tgaatctgga
    47461 gacatgactg gcagaataag aggcaatcct tgagcatcta aaactgcatc cacgaatcct
    47521 tgcggtgtat agctcacttg atttccttgg aaagtatctg ttgctcgaat cagttgattt
    47581 ccggcaatgc cgatgattgg tttcatttgt attcccccta aagtagactt attgtaacaa
    47641 ggatttaaaa aaaattaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg
    47701 ttactatctg taaagagaaa tttcgtgtga aattcatgac gatagttgaa aatattgctt
    47761 aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct
    47821 agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct
    47881 ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg
    47941 aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaaa
    48001 agcgtgctca aacactgcgg cagcagctga agatctatgc aacagaaaaa gagcaggaat
    48061 tggaaacgat ccaaacaaac tatcgacaac aactaaaaca gttggtggaa gaaaaagaac
    48121 aatttgaaat acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg
    48181 tgcaagcaac tttgaaagat caatatcaaa agcatcagag tcaagcgatc ggtgcgatca
    48241 ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca
    48301 gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa cgttgaaatc
    48361 cgggatctgt atcaaaatag aacaaacaac cagtgggtgg agaagtattt tccagatacc
    48421 acgattattg ataaagaagc aaaattaagc gctttaggta atcggttgaa agacatccaa
    48481 gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa
    48541 cgtcaagaac tttccttaca agatttggaa gccaattatt ccgaagaaaa tttcataaat
    48601 aaattgaaag aagtccttga tttaaaagaa aagtgggaac aactaactga agaacgtgat
    48661 cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt
    48721 atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa
    48781 agtttcaagg aagaactgag taagcttgat gacgtttata tggaagaaat agattatacg
    48841 ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgt tatagaagaa
    48901 attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa
    48961 aaactgcaag agaccaagca gcaattatcc attgttcacg agcagcaaaa gaagataacg
    49021 acagcgttag gtcagtgcag tggctatatc caagaatttg aatgggtaga agaagtcaca
    49081 cttgccgttg cagaacgaga aaaaataaaa gaacggttta tccatgcgac ttatctgatc
    49141 gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt gttaaacgaa
    49201 accgtatcag aagaagatat atacgtcagt ttcgaaacac caaactcaga agagattcag
    49261 caggaagtcc ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga attgctgact
    49321 gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa caccttggat gacaccattt
    49381 tatcttgtct tcttcgggat gatggtcgca gatgttggtt atggtttgct gatgctcatt
    49441 gggacaatac tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg tttcgtaaaa
    49501 ttctttgaga ttctctcgat accatcgatc atctggggat tgatctacag ttcatttttc
    49561 ggacagtcat taccaaaaga actgtttggc atccgcttgc ctttcccaat cctatctacg
    49621 acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat acagattttg
    49681 gttggattat ttgtggcagc aaaggaaaat atcaggagaa aagattactt gagtgctgtc
    49741 agtgatggat ttgcctggca agggatccta attgggatcg ttattgcgtt ggttggtgcc
    49801 atgttgataa aaaatacagc atttgtttac cttggcgctg ggctatcgat cattgctgcc
    49861 ttatgtatct tgatcgttcc gattatccaa acgccgtcta aagtcaaagg tgctgccaaa
    49921 ggtgcgtata atctttacgg actgaccggc tatatcggtg atttagtcag ttatacacgt
    49981 ttgatggcat tgggtatttc aggcggaagt attggagcgg cattcaatat gctagttgct
    50041 ttcatgccgc cagctgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca
    50101 ctgaatatgt ttttgacact gctaagtgcc tatgtccatg gcgcacgatt gcagtatgtc
    50161 gaattttttg ggaagtttta tacaggaggc ggacgtgcct ttgatccgct gaaaacggcg
    50221 gaaaaatacg tcaacatcaa tcacaagaaa aaaaacaata aataattgga ggatttttat
    50281 ataatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcggt attagcgatg
    50341 gcaacagcga cgattttttc agggatcggt tcagctaaag gtgttggaat gacaggtgag
    50401 gcagcagcag cattgacaac aagtcaaccc gaaaaattcg ggcaggctct gattctgcaa
    50461 ttgcttccag gcacgcaagg tttgtatgga ttcgttattg cctttttgat ttttatcaac
    50521 ttaggaaatg acatgtcagt tgtacaagga ctgaactttc taggtgcttc tctgccgatt
    50581 gcttttacag gcttgttttc aggtattgcc caaggaaaag tcgcagcggc aggtattcaa
    50641 attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa
    50701 acatacgcga ttttaggttt cgttatttcc ttcctattag tgttgaatgc ctaagcataa
    50761 aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaac gaagcagcag
    50821 agcaggaaag agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa aactttgaac
    50881 aaaaacgtac gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac
    50941 tcgaaaaaaa ataccgtcag ctaaggaatc gccagcaagt agaagtacga caagaaaatt
    51001 tgaatgcgaa gcaggaattt ttaagacgat tgtttgcaga tgcggtaact gaaatggaaa
    51061 attgggatga atctgaacaa atccagttta tcaaaaatgc attgtattca ttacctttga
    51121 caggaaaagt agcattcatt gctggagaaa aatcagcagc ctatctttct cagacgttgt
    51181 tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg
    51241 atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc
    51301 ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag
    51361 gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga
    51421 acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg
    51481 tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt
    51541 cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc
    51601 ggagcctgaa atcgtttgga tctatacgat gcgttatact ttccataatc tgaaggtttt
    51661 gacaaaagct gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta
    51721 ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac ttcctgaaag
    51781 cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg
    51841 aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct
    51901 aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac
    51961 gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag
    52021 ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta
    52081 cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg
    52141 aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca
    52201 agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga
    52261 ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaatcaga
    52321 aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca
    52381 aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc
    52441 aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca
    52501 cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac
    52561 ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc
    52621 aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa
    52681 ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt
    52741 ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta
    52801 tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc
    52861 caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca
    52921 tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa
    52981 gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt
    53041 tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag
    53101 aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca
    53161 aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa
    53221 atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca
    53281 aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct
    53341 tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag
    53401 tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg
    53461 gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta
    53521 atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag
    53581 tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca
    53641 tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg
    53701 agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt
    53761 cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc
    53821 gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac
    53881 cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag
    53941 cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag
    54001 aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg
    54061 gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg
    54121 attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt
    54181 atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca
    54241 aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg
    54301 cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca
    54361 aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa
    54421 cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc
    54481 ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa
    54541 ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa
    54601 gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct
    54661 tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga
    54721 gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat
    54781 ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc
    54841 caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca
    54901 gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca
    54961 acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt
    55021 gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc
    55081 atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct
    55141 ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca
    55201 gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca
    55261 ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca
    55321 ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa
    55381 gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc
    55441 ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt
    55501 tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg
    55561 atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt
    55621 ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt
    55681 gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat
    55741 ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac
    55801 atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat
    55861 ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat
    55921 aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag
    55981 aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta
    56041 ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa
    56101 aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat
    56161 tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt
    56221 gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg
    56281 acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga
    56341 gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt
    56401 atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg
    56461 ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca
    56521 aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga
    56581 ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg catgggacaa
    56641 attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat
    56701 tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga
    56761 tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag
    56821 tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg
    56881 tgatgaagct catgctttac atcctaaaat tggcctttgt gatccaattg attggtacgg
    56941 ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt
    57001 tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag
    57061 ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg
    57121 ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga
    57181 ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt
    57241 tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg
    57301 caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc
    57361 tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat
    57421 ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc
    57481 gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg
    57541 cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca
    57601 tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg
    57661 aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg
    57721 caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg
    57781 tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag
    57841 aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa
    57901 gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt
    57961 tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca
    58021 ataagatttg attcctttat ttatcaatag gttataattc atattttttt attttttctt
    58081 ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa
    58141 cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt
    58201 tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc
    58261 gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat
    58321 tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga
    58381 ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg
    58441 cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag
    58501 aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga
    58561 aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa
    58621 taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga
    58681 tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt
    58741 ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa
    58801 cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg
    58861 ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa
    58921 caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat
    58981 ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa
    59041 caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt
    59101 caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg
    59161 tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca
    59221 aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat
    59281 aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa
    59341 tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt
    59401 ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt
    59461 aaaattgtat accatacaat tttgccattg attgtattga ttttagaagt taatcttttg
    59521 tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt
    59581 ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca
    59641 gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga
    59701 tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc
    59761 cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc
    59821 aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg
    59881 ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa
    59941 ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca
    60001 ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta
    60061 aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc
    60121 cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg
    60181 taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa
    60241 gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag
    60301 aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg
    60361 gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga
    60421 cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata
    60481 acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc
    60541 agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga
    60601 aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa
    60661 gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg
    60721 tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac
    60781 gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta
    60841 catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc
    60901 gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga
    60961 tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg
    61021 aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt
    61081 tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc
    61141 ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg
    61201 cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt
    61261 gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg
    61321 gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt
    61381 aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta
    61441 aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga
    61501 caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccag
    61561 cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat
    61621 agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt
    61681 catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc
    61741 ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca
    61801 ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc
    61861 ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt
    61921 aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg
    61981 gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa
    62041 gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg
    62101 aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg
    62161 gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt
    62221 atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta
    62281 taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg
    62341 tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa
    62401 actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat
    62461 tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct
    62521 tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg
    62581 gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc
    62641 tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa
    62701 tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt
    62761 gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt
    62821 gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc
    62881 gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga
    62941 tgactgccga cacggcgtgc cattacttcg atctctagga aatcttcatg tgtcagtccg
    63001 tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata
    63061 acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag
    63121 ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct
    63181 tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg
    63241 actagtttga tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca
    63301 ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc
    63361 aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc
    63421 gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc
    63481 aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga
    63541 tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac
    63601 aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt
    63661 ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac
    63721 cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta
    63781 aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct
    63841 tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg
    63901 gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc
    63961 actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt
    64021 aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc
    64081 aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg
    64141 gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta
    64201 agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga
    64261 ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac
    64321 agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt
    64381 tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct
    64441 aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat
    64501 tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga
    64561 tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt
    64621 tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct
    64681 ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc
    64741 ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc
    64801 ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac
    64861 tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat
    64921 gacttgccca cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc
    64981 atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata
    65041 agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc
    65101 gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc
    65161 actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc
    65221 acctgaattt ctgatcactg gagcgtaacc tgcatcttcc agtgtttgga tcccgtcact
    65281 tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa
    65341 atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa
    65401 aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca
    65461 accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt
    65521 gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact
    65581 aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca
    65641 ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat
    65701 tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag
    65761 attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat
    65821 gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg
    65881 ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa
    65941 ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga ttatgatctg
    66001 ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata
    66061 taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag
    66121 gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg
    66181 atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta
    66241 gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca
    66301 caattttata ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg
    66361 ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac
    66421 gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat
    66481 gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa
    66541 gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat
    66601 aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca
    66661 ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa
    66721 gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga
    66781 cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt
    66841 tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc
    66901 accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata
    66961 agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt
    67021 atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg
    67081 tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc
    67141 attttagtgc atggttttat cggatattga tccgcacggc attagatagt tggcgcagac
    67201 aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag
    67261 aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga
    67321 ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata
    67381 tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc
    67441 tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag
    67501 aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt
    67561 attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc
    67621 ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt
    67681 cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa
    67741 cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat
    67801 gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca
    67861 gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg
    67921 ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc
    67981 caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt
    68041 gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa
    68101 gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact
    68161 gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt
    68221 ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct
    68281 ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca
    68341 tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca
    68401 agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa
    68461 attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg
    68521 tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat
    68581 ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca
    68641 aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag
    68701 acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg
    68761 gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg
    68821 ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag
    68881 ctgaagcagg gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt
    68941 ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg
    69001 gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga
    69061 tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc
    69121 ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc
    69181 atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc
    69241 aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact
    69301 attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata
    69361 acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc
    69421 tatacaggta caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa
    69481 aataaagaag caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga
    69541 atcgaacccc cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa
    69601 gcactgggat aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg
    69661 aaatttgagc aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa
    69721 cagttgcaac agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt
    69781 gagaatcaag caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat
    69841 tacataaaag cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt
    69901 cctgatacaa aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga
    69961 gatacttttt tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt
    70021 ttgacgatta gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc
    70081 gatgcgttga ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa
    70141 gaatgtctca tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg
    70201 ttggaagaat gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt
    70261 tctgttgatc tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc
    70321 cctggccgga ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta
    70381 gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc
    70441 tttcaagaga gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat
    70501 ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat
    70561 acaattttgc gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag
    70621 gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa
    70681 tccacggtca gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg tgtatttgaa
    70741 ttaaaaaaat ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt
    70801 tcagcagata cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag
    70861 ccgttgtctg accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga
    70921 cgaacagtag caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt
    70981 tatgataact aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag
    71041 ttgaaaaacg agagcaaaac tgttagactt acagtcgtga ggttgagatt tccctttatt
    71101 gatgtgaaat tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac
    71161 attggacgta aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga
    71221 atctgttgca ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat
    71281 ctattggatg cagccgattg gacgtcgaag tttatcggaa agtatgggga tcacagaacg
    71341 agtattgcga acagaaacag atcttttgaa aaatctgaat ttgatcgaca cctcaaaaag
    71401 cggtatgacg ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga
    71461 tcagctttta ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg
    71521 ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga
    71581 agcgctgagc gaagccctag accgactact tccagaaggc gagaatatca tcgctgtgat
    71641 gggaggaact actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg
    71701 gcataacctg tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa
    71761 ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac caccgtgcac tttatgttcc
    71821 ggaacaatta agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt
    71881 cttaaacttg attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat
    71941 ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt
    72001 tgcggaatct tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag
    72061 aatcggcata gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg
    72121 aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct
    72181 catcactgat gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata
    72241 tttttttgat tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt
    72301 ttggacgtat tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag
    72361 tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa
    72421 ctcaaggacg tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa
    72481 aagaagttaa agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg
    72541 tagatatcgt tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt
    72601 taacagctgg tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa
    72661 tcgtttataa cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt
    72721 catgtactac taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg
    72781 ttgaaggatt aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac
    72841 ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact
    72901 caacaggtgc tgctaaagct atcggtttag taattcctga attgaacggt aaattagacg
    72961 gagctgctca acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg
    73021 aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt
    73081 acggatacaa cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat
    73141 tatttgatgc tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg
    73201 ttgcttggta cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact
    73261 tcgctaactt ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa
    73321 gcggggaagc aatcgcgctt cttccgcttt ttttgataat aagtttatt
//
LOCUS       DAZKMK010000005        56128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000005 DAZKMK010000000
VERSION     DAZKMK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.005"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..675
                     /locus_tag="ACG8V1_000344"
     CDS             157..675
                     /locus_tag="ACG8V1_000344"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0328468.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            803..1486
                     /locus_tag="ACG8V1_000345"
     CDS             803..1486
                     /locus_tag="ACG8V1_000345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0328469.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1795..1868
                     /locus_tag="ACG8V1_000346"
     tRNA            1795..1868
                     /locus_tag="ACG8V1_000346"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1829..1831,aa:Arg,seq:tct)
     gene            2027..2386
                     /locus_tag="ACG8V1_000347"
     CDS             2027..2386
                     /locus_tag="ACG8V1_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0328470.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2509..4140
                     /locus_tag="ACG8V1_000348"
     CDS             2509..4140
                     /locus_tag="ACG8V1_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0328471.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4202..4843)
                     /locus_tag="ACG8V1_000349"
     CDS             complement(4202..4843)
                     /locus_tag="ACG8V1_000349"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0328472.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5029..5105
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5125..5433
                     /gene="rplU"
                     /locus_tag="ACG8V1_000350"
     CDS             5125..5433
                     /gene="rplU"
                     /locus_tag="ACG8V1_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0328473.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5460..5804
                     /locus_tag="ACG8V1_000351"
     CDS             5460..5804
                     /locus_tag="ACG8V1_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0328474.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5817..6110
                     /gene="rpmA"
                     /locus_tag="ACG8V1_000352"
     CDS             5817..6110
                     /gene="rpmA"
                     /locus_tag="ACG8V1_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0328475.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6544..9351
                     /locus_tag="ACG8V1_000353"
     CDS             6544..9351
                     /locus_tag="ACG8V1_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0328476.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9542..10033
                     /locus_tag="ACG8V1_000354"
     CDS             9542..10033
                     /locus_tag="ACG8V1_000354"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0328477.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10136..11125
                     /locus_tag="ACG8V1_000355"
     CDS             10136..11125
                     /locus_tag="ACG8V1_000355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0328478.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11158..11961
                     /locus_tag="ACG8V1_000356"
     CDS             11158..11961
                     /locus_tag="ACG8V1_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0328479.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11982..12896
                     /locus_tag="ACG8V1_000357"
     CDS             11982..12896
                     /locus_tag="ACG8V1_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0328480.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12979..13353
                     /locus_tag="ACG8V1_000358"
     CDS             12979..13353
                     /locus_tag="ACG8V1_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0328481.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13492..13737
                     /locus_tag="ACG8V1_000359"
     CDS             13492..13737
                     /locus_tag="ACG8V1_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0328482.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13909..14973
                     /locus_tag="ACG8V1_000360"
     CDS             13909..14973
                     /locus_tag="ACG8V1_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0328483.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15219..15644
                     /locus_tag="ACG8V1_000361"
     CDS             15219..15644
                     /locus_tag="ACG8V1_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0328484.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15637..16086
                     /gene="nusB"
                     /locus_tag="ACG8V1_000362"
     CDS             15637..16086
                     /gene="nusB"
                     /locus_tag="ACG8V1_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0328485.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16183..17037
                     /locus_tag="ACG8V1_000363"
     CDS             16183..17037
                     /locus_tag="ACG8V1_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0328486.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17038..18375
                     /gene="xseA"
                     /locus_tag="ACG8V1_000364"
     CDS             17038..18375
                     /gene="xseA"
                     /locus_tag="ACG8V1_000364"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0328487.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18380..18604
                     /locus_tag="ACG8V1_000365"
     CDS             18380..18604
                     /locus_tag="ACG8V1_000365"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0328488.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18606..19496
                     /locus_tag="ACG8V1_000366"
     CDS             18606..19496
                     /locus_tag="ACG8V1_000366"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0328489.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19505..20323
                     /locus_tag="ACG8V1_000367"
     CDS             19505..20323
                     /locus_tag="ACG8V1_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0328490.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20430..20879
                     /locus_tag="ACG8V1_000368"
     CDS             20430..20879
                     /locus_tag="ACG8V1_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0328491.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20897..22579
                     /gene="recN"
                     /locus_tag="ACG8V1_000369"
     CDS             20897..22579
                     /gene="recN"
                     /locus_tag="ACG8V1_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0328492.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22634..22750)
                     /locus_tag="ACG8V1_000370"
     CDS             complement(22634..22750)
                     /locus_tag="ACG8V1_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0328493.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22823..23779)
                     /locus_tag="ACG8V1_000371"
     CDS             complement(22823..23779)
                     /locus_tag="ACG8V1_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0328494.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23950..24318
                     /locus_tag="ACG8V1_000372"
     CDS             23950..24318
                     /locus_tag="ACG8V1_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0328495.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24488..24919
                     /gene="mraZ"
                     /locus_tag="ACG8V1_000373"
     CDS             24488..24919
                     /gene="mraZ"
                     /locus_tag="ACG8V1_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0328496.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24940..25899
                     /gene="rsmH"
                     /locus_tag="ACG8V1_000374"
     CDS             24940..25899
                     /gene="rsmH"
                     /locus_tag="ACG8V1_000374"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0328497.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25904..26305
                     /gene="ftsL"
                     /locus_tag="ACG8V1_000375"
     CDS             25904..26305
                     /gene="ftsL"
                     /locus_tag="ACG8V1_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0328498.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26306..28498
                     /locus_tag="ACG8V1_000376"
     CDS             26306..28498
                     /locus_tag="ACG8V1_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0328499.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28524..29486
                     /gene="mraY"
                     /locus_tag="ACG8V1_000377"
     CDS             28524..29486
                     /gene="mraY"
                     /locus_tag="ACG8V1_000377"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0328500.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29500..30861
                     /gene="murD"
                     /locus_tag="ACG8V1_000378"
     CDS             29500..30861
                     /gene="murD"
                     /locus_tag="ACG8V1_000378"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0328501.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30887..31975
                     /gene="murG"
                     /locus_tag="ACG8V1_000379"
     CDS             30887..31975
                     /gene="murG"
                     /locus_tag="ACG8V1_000379"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0328502.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31990..33210
                     /locus_tag="ACG8V1_000380"
     CDS             31990..33210
                     /locus_tag="ACG8V1_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0328503.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33366..34691
                     /gene="ftsA"
                     /locus_tag="ACG8V1_000381"
     CDS             33366..34691
                     /gene="ftsA"
                     /locus_tag="ACG8V1_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0328504.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34714..35955
                     /gene="ftsZ"
                     /locus_tag="ACG8V1_000382"
     CDS             34714..35955
                     /gene="ftsZ"
                     /locus_tag="ACG8V1_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0328505.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35972..36649
                     /locus_tag="ACG8V1_000383"
     CDS             35972..36649
                     /locus_tag="ACG8V1_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0328506.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36663..37262
                     /locus_tag="ACG8V1_000384"
     CDS             36663..37262
                     /locus_tag="ACG8V1_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0328507.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37343..37564
                     /locus_tag="ACG8V1_000385"
     CDS             37343..37564
                     /locus_tag="ACG8V1_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0328508.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37613..38395
                     /locus_tag="ACG8V1_000386"
     CDS             37613..38395
                     /locus_tag="ACG8V1_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0328509.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38416..39123
                     /locus_tag="ACG8V1_000387"
     CDS             38416..39123
                     /locus_tag="ACG8V1_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0328510.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39385..42183
                     /gene="ileS"
                     /locus_tag="ACG8V1_000388"
     CDS             39385..42183
                     /gene="ileS"
                     /locus_tag="ACG8V1_000388"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0328511.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42232..43758)
                     /gene="zwf"
                     /locus_tag="ACG8V1_000389"
     CDS             complement(42232..43758)
                     /gene="zwf"
                     /locus_tag="ACG8V1_000389"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0328512.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43773..44420)
                     /locus_tag="ACG8V1_000390"
     CDS             complement(43773..44420)
                     /locus_tag="ACG8V1_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0328513.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44604..44933)
                     /locus_tag="ACG8V1_000391"
     CDS             complement(44604..44933)
                     /locus_tag="ACG8V1_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0328514.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45110..45838
                     /gene="sfsA"
                     /locus_tag="ACG8V1_000392"
     CDS             45110..45838
                     /gene="sfsA"
                     /locus_tag="ACG8V1_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0328515.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45854..46867
                     /locus_tag="ACG8V1_000393"
     CDS             45854..46867
                     /locus_tag="ACG8V1_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0328516.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46867..48144
                     /locus_tag="ACG8V1_000394"
     CDS             46867..48144
                     /locus_tag="ACG8V1_000394"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0328517.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48207..50909
                     /locus_tag="ACG8V1_000395"
     CDS             48207..50909
                     /locus_tag="ACG8V1_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0328518.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51061..51378
                     /locus_tag="ACG8V1_000396"
     CDS             51061..51378
                     /locus_tag="ACG8V1_000396"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0328519.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51408..51728
                     /locus_tag="ACG8V1_000397"
     CDS             51408..51728
                     /locus_tag="ACG8V1_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0328520.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51836..53296
                     /locus_tag="ACG8V1_000398"
     CDS             51836..53296
                     /locus_tag="ACG8V1_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0328521.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53364..53585
                     /locus_tag="ACG8V1_000399"
     CDS             53364..53585
                     /locus_tag="ACG8V1_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328522.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53616..53798
                     /locus_tag="ACG8V1_000400"
     CDS             53616..53798
                     /locus_tag="ACG8V1_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0328523.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53798..54211
                     /locus_tag="ACG8V1_000401"
     CDS             53798..54211
                     /locus_tag="ACG8V1_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0328524.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54334..55515
                     /locus_tag="ACG8V1_000402"
     CDS             54334..55515
                     /locus_tag="ACG8V1_000402"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0328525.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55586..55750)
                     /locus_tag="ACG8V1_000403"
     CDS             complement(55586..55750)
                     /locus_tag="ACG8V1_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328526.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55904..55977
                     /locus_tag="ACG8V1_000404"
     tRNA            55904..55977
                     /locus_tag="ACG8V1_000404"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55938..55940,aa:Arg,seq:cct)
     gene            complement(56046..>56128)
                     /locus_tag="ACG8V1_000405"
     CDS             complement(56046..>56128)
                     /locus_tag="ACG8V1_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0328527.1"
                     /translation="HIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        18566 a         9469 c        12091 g        16002 t
ORIGIN      
        1 cgtcaattat ttataataaa aaatgagata aagatgagga aatagaatta gaaacatata
       61 attattaaag ggaaacattt ttacagctat aatttgaaaa aatttatcgt tattatatga
      121 ttaagaagga ttgaataaag aaagaagttg aaaacgatac ctgattacaa aaaaataact
      181 tttataagtt ctataacgtt gattatattt ttaattctta ttcttctcgt cgtcttggtt
      241 ccccgaattt ttggtttgac tccttatatt attgaggaca aagaaatgag tccatattat
      301 gcacaaggaa atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat
      361 attattacat attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt
      421 gaaaacaacc acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga
      481 gtagtaaaaa agagaaatat tatcggtagt cccatctttc agattccata tattggacta
      541 gtcctttcaa aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc
      601 ctaacaggaa tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca
      661 cagaaatttt tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc
      721 tgaacatgaa acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata
      781 atgtgtgaag aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga
      841 agaactattg gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact
      901 ggaagaagaa gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt
      961 gaaattttta cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg
     1021 tttttcatca agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga
     1081 tcgtttcgat gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga
     1141 taaagtgact ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta
     1201 tgactttatt tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt
     1261 acgtttgtta agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat
     1321 cttgctccca gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga
     1381 atttcttcgt gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga
     1441 tggtgtgatc ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc
     1501 agcctgtaga taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat
     1561 atttgtaagt caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa
     1621 tgtagctcct gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa
     1681 aagattgatt tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc
     1741 gtattgacag aagcaattta attatctata attattgatg ttccgctgat tcttgtcata
     1801 gtagctcagc tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc
     1861 ctgtgacgct aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt
     1921 gccaatattg tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat
     1981 tataaggagc aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc
     2041 tagaatttta gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt
     2101 gagaactgat tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag
     2161 aaaatctgtt caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg
     2221 actactttat ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat
     2281 atctattttt tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa
     2341 acaaggtggg ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata
     2401 agaatttatc taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat
     2461 tctaatgaat ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat
     2521 tatgtttcct cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat
     2581 cttttggctg ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta
     2641 tcaagcggag ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt
     2701 ggcggtttat caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc
     2761 ttgatgtcta ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg
     2821 ggtctatttt tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca
     2881 gcacccgtaa ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc
     2941 gataatttat ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat
     3001 caaacgatag gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta
     3061 gggatgttta tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc
     3121 attttggcaa cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt
     3181 ccacaaacat tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg
     3241 caacaagtgg cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta
     3301 tgtggtgctt cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg
     3361 gcgcaaggaa ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc
     3421 atcgcccgta caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata
     3481 catgcagttg tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca
     3541 atgccagcac ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg
     3601 atcaaggagc tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg
     3661 atttgtacag ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt
     3721 gtattttttg ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa
     3781 agaatggggc tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt
     3841 ccactgtttt ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac
     3901 gatggtgtca ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt
     3961 tttgatgaat ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg
     4021 cagccagaag tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat
     4081 caaacagttg ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa
     4141 aacaatgaaa aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt
     4201 tttaatattg ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa
     4261 gggagcgatt gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt
     4321 ctgaccattc gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt
     4381 gaccatgatt tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc
     4441 caagtgtcgc aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat
     4501 gcgcaaatac ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg
     4561 tccgtttatt gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat
     4621 acacctcttc tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt
     4681 gaccgctaaa aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca
     4741 tcagtttagg atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca
     4801 ttcttaaaga agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt
     4861 cttttattat aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg
     4921 atagaaaagc agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg
     4981 actaacggtt gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa
     5041 ccgctcagaa caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta
     5101 tatcagaagg aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta
     5161 aaagttgaag tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa
     5221 gttgtttttg acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact
     5281 gttgcaggtg caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt
     5341 actttcaaat acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat
     5401 acaaaagtgg ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata
     5461 tgatcaaagg aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg
     5521 ggcatgctga tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag
     5581 cgatcagcac tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga
     5641 atgaagaaga aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa
     5701 acaatattgc tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg
     5761 aaaactcaga atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt
     5821 tgttatctat gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg
     5881 gccgtgactc agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag
     5941 gtggatcaat tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta
     6001 tcggtggaga cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag
     6061 gccgtaacaa aaaacaagtg tctgtttatc cagtagctca agaagcttaa ttgaatctta
     6121 tgattaaaga ggttgtgaca agacttttgt cacagccttt tttgtttata aaaataaaag
     6181 ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt tttgccaatt
     6241 tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata ttgtttgcta
     6301 tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta taaaaatcgt
     6361 atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt tatcaacaaa
     6421 acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat gttttgaagc
     6481 ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat tgaccgaatt
     6541 tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga tcaaggagta
     6601 acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag taaagatctg
     6661 aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt acgttacata
     6721 tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa
     6781 acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat ctttgcaaaa
     6841 atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc tgcgattttg
     6901 tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg aaagacttat
     6961 tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa
     7021 ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat gagtcattta
     7081 tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg gattatcgat
     7141 caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc tgaaggtcaa
     7201 gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga aacaacgaaa
     7261 tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg ttcttctcta
     7321 ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt
     7381 cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc aaaccgcacg
     7441 cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag tgtgacctat
     7501 ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg atttctaaat
     7561 catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg gattcgtagc
     7621 ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa aatattggag
     7681 ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga ctcctatgag
     7741 ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa gtcggatggt
     7801 ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca tctgaagtct
     7861 ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga atttgtagat
     7921 ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc tttaccatat
     7981 gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt tttgaaaaaa
     8041 atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc ttttgatttt
     8101 ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg
     8161 gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt acgggcaaat
     8221 caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt
     8281 atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt agacatgcct
     8341 ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca agaagtagat
     8401 gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt taattcgcaa
     8461 attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac ttctctagtt
     8521 ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt gcatgaatca
     8581 ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca ggaagcaaaa
     8641 cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg tacagcacaa
     8701 agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga attagaagtt
     8761 ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa agaatatcag
     8821 atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat tcctttagaa
     8881 cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga atcagaattg
     8941 gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg tgttgagttt
     9001 atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat gtggcaattt
     9061 tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt caaaatcaac
     9121 ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc attgactgtg
     9181 gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt ggcagcctca
     9241 gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat gtattatctg
     9301 ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg aatacacaaa
     9361 aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct ttaaattcaa
     9421 ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg
     9481 gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag gagttttgca
     9541 gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag tcgcaacagt
     9601 ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga
     9661 taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag caaatgtaat
     9721 aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag caaaagtgat
     9781 gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg tacagttgaa
     9841 gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta ccaattttgt
     9901 ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag aagtggagtt
     9961 agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac tagcgaaaat
    10021 caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt gtaatggtta
    10081 gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta taaatatggt
    10141 agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat ccggttcaat
    10201 gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga
    10261 tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag acgaagtgtt
    10321 attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat tattcgaaga
    10381 gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga ttgaagccta
    10441 cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca ttgaaacagc
    10501 aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac cgaaagcagc
    10561 agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg gagacggaaa
    10621 aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag ttgcgacagc
    10681 ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag tatcaaagga
    10741 tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat
    10801 tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata ccaaagcatt
    10861 gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg tagacatcaa
    10921 agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt
    10981 gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt
    11041 cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt tgaaaaaagc
    11101 aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag gcttatcatg
    11161 tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat ggaaggtatt
    11221 cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg tttagttacc
    11281 ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc tttaggttgg
    11341 gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc atcagcaatt
    11401 attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt
    11461 cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc tgttccaatc
    11521 gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac
    11581 atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc gttgctattt
    11641 atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt aacagatggt
    11701 atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat caacatgatg
    11761 gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc aatttctcaa
    11821 ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta cttgaacctt
    11881 tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga ccctttaggc
    11941 gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa gaaaaaatca
    12001 aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt caaggttcat
    12061 ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca gcaatcaaaa
    12121 aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg gaattcttta
    12181 atacacaccc atacattgct tctccaattt taggggttac attggctctg gaagaagaac
    12241 gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta ggtatgatgg
    12301 gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca atgctaggtg
    12361 cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc ttcttcttag
    12421 gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc tacaaagctg
    12481 gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca aaaggtgctt
    12541 caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca atcaaattct
    12601 taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg gataaattgc
    12661 cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc ttagcccttt
    12721 ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct ggtttagctg
    12781 cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta
    12841 tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt ttataatcca
    12901 taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg ttcataaaca
    12961 aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt ggtaattgat
    13021 gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtaa taatggtttt
    13081 gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga agaagtggat
    13141 tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc cattcaaacg
    13201 aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc
    13261 agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga tgtagtgaaa
    13321 cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc taagaggagg
    13381 ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca attatttgtc
    13441 ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat tatgagttgg
    13501 ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat caccagtaaa
    13561 ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc aagtattgga
    13621 caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat tccatctatt
    13681 ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg atcataagtt
    13741 tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt ttggctttat
    13801 ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg tacgaagaaa
    13861 agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat gatgctacgt
    13921 gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt ggttaccagt
    13981 ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc tgttattact
    14041 ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc agcgcaggct
    14101 caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc aaacttagta
    14161 cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt tttagaatat
    14221 tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat gatcgaagaa
    14281 ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg tcatattgcg
    14341 gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga gattgaagtc
    14401 gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc ttttgatacg
    14461 atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc
    14521 gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta cgtttccgat
    14581 atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat ttatcaaata
    14641 gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac tggtgtccaa
    14701 ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc atttggacat
    14761 tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc ttctcgtgca
    14821 gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta cttaccagga
    14881 atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg
    14941 actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa gtaagcgtaa
    15001 aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata cactatagaa
    15061 gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca tagcagtttg
    15121 cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat agagaattcg
    15181 gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa aaaaatttga
    15241 ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa
    15301 ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga acttttgcca
    15361 gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg
    15421 aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt tcagttccta
    15481 aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg acggatatcg
    15541 atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa
    15601 caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt atcaagacac
    15661 gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc agacctaaca
    15721 aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat caacgaagat
    15781 gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa
    15841 gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat caatcggatc
    15901 tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta tgtgacagat
    15961 gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt tagtgatgac
    16021 cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt agaagctggg
    16081 gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc gggaaaattc
    16141 tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga attgattaac
    16201 ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca aaaattgaaa
    16261 gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc agccagccaa
    16321 atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc attggtagag
    16381 cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa
    16441 gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat agatgaagaa
    16501 aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc gatgaattta
    16561 ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg catcatgaaa
    16621 atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat cggacgaagc
    16681 aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac tgttactgtt
    16741 gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat tttagtcgta
    16801 gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata
    16861 gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa
    16921 gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc aatgactatc
    16981 acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg tgaatagatg
    17041 gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa atttgatgcc
    17101 gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg tttacgtgcc
    17161 aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc tattatgttc
    17221 aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt
    17281 gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga gcacatggag
    17341 cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag
    17401 gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc
    17461 cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa aagaagatat
    17521 ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc agcggacaat
    17581 gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat gatcatcggt
    17641 cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg
    17701 atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga taccacgatc
    17761 gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga attagctgtt
    17821 cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt gcagcaggcg
    17881 tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg ttcctatgta
    17941 tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca gttgaaacag
    18001 cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac gatcgcatta
    18061 agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg ccaagaaact
    18121 gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca acagcagcag
    18181 cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat catgggaaga
    18241 ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa
    18301 ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt acaagagata
    18361 gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc aagaattaga
    18421 aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc tagattcgtt
    18481 taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag cagaagagac
    18541 cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa acgaggaaag
    18601 ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa aaagaaatct
    18661 tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg agttattcag
    18721 tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt gcttcctttg
    18781 atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg gtgcatacgt
    18841 attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt agaggaaaac
    18901 ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat ggtttgctta
    18961 caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag cttctgctgc
    19021 ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa gctgcagata
    19081 tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat gaaagaaaga
    19141 cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag caacctgaag
    19201 aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt cagataagag
    19261 atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct ggtaaagatg
    19321 aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt
    19381 tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag gacgtttttt
    19441 cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag aaatgaggga
    19501 aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat ttgaaacaag
    19561 agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa acgagcggtt
    19621 tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag gtaaaaaact
    19681 tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat ttgatctatc
    19741 tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta ctgatgcagc
    19801 tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc agctagcttg
    19861 gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa
    19921 acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt cttttatttc
    19981 actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag aagtgattgc
    20041 tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg gaattgttcg
    20101 tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg cacaaggtgg
    20161 atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa atatcgagtt
    20221 cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta ttaatatggc
    20281 agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat
    20341 caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta tcattgatta
    20401 agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta
    20461 ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa atcctgaaaa
    20521 gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa ctgaaactga
    20581 tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa acaaacgaag
    20641 atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt gaccaaatgg
    20701 aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca aatttgattg
    20761 ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat gttttgatga
    20821 ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat tacctttaag
    20881 ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga ttattccgga
    20941 attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa
    21001 atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa gcgattatat
    21061 acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa aacaagaagg
    21121 attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc tgattgttcg
    21181 acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt
    21241 agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa acgaacatca
    21301 ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag atgtagcttt
    21361 ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc tggaaaaaag
    21421 ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata tgctccattt
    21481 ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga aattaaaaga
    21541 agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg caggttacgg
    21601 agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg tttcagaact
    21661 gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata tccaaagtgc
    21721 ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt tattggagtt
    21781 agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac agttgaaacg
    21841 taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca ccgaagagct
    21901 agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa
    21961 agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa
    22021 agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa acactgagtt
    22081 tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat ttgaccaaat
    22141 cgaattttac attacgacca atccaggaga gccactgaag cctttggtga aagtcgcttc
    22201 tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt ctgagcaagg
    22261 tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag ttgcacaggc
    22321 catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca tcactcatct
    22381 gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag tgattgatgg
    22441 acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag aaattgcacg
    22501 tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag aactattgaa
    22561 actcgcaaaa aggacctgag ccacctcgca gtggttcagg tccttttcag cagctgatag
    22621 ctatatttta tttttacatg acactcatta cagcagtcaa tacaacagat ccaatcgtta
    22681 aaatcagcat tgtccaaatg acaaattttg ttactttagc aaaagtactt gtttgttttt
    22741 tttcacgcat tgttttcaac ctcgtcatta attagttgat tcaagtgtac aatctttttt
    22801 ccatttccac aagagggctt ttttataaaa acttcccttt ttttaaataa taggtagtta
    22861 cgatacaaat aaataatgtt aatagaacta gccatataaa ggcatcatcc ctttcagcaa
    22921 taggtaactt aatgttcatt ccatagatac tgccgacgat tgtcggtata gttaaaataa
    22981 tcgttaaaga agttaaaatt ttcataatat tattcagatt attcgatacg attgctgaaa
    23041 aagtctgatt catttgttct actagtttta attgaatgcg agaagtagtc attgcctgct
    23101 ctgtttccac caatacatca cgtaaatgat ttttcaaagc atgattttct tggaaaagtt
    23161 tattttgcgc tagttctttc aatgcatcta aattagcaga agtcgcttct ctgaaataaa
    23221 cgaggctttt ttggatatcc atgatttgat aaagttgttt gttttcagtg gatacttgaa
    23281 gtttggcttc caaagtatcg cattgagatt gaagcgtagc taaataactg ttgtaagaga
    23341 gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt aggcgaaata tcactttttg
    23401 gaaaggtctg gctaaagact ttctttaaaa acagtggttc atgattcgta attgtgataa
    23461 gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt atccacttga aaataaccgc
    23521 taggacttgt tttagcaaaa ggatattgta acaataataa cgctggcttt gtcaactttg
    23581 attgattgaa ttcttctgta cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat
    23641 ctgacggaat ttcaaattga tgtgtcagct gatgaatctc ttcttgatta ggacgttcta
    23701 cacaatacca gatcgtttgg tcagtttcct ttccggtatt acgcagtttc tcattatcta
    23761 atgtaaaata tttgatcatg attttcctcc tgttgcctgt ttcttttatt ataacggata
    23821 gggaattgga atacatggaa tgtgtttgta agatatagaa tataactgca taaatttaaa
    23881 taaaatgaaa cagttgtgta aagtatatac agtatacttg aagcaacgat aaaaaaggag
    23941 tttacgttca tgggcacatt cacaccactt atcttgtttt ggtatattta cccggtaatc
    24001 gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata
    24061 aaagcccctg atttggcgat tccttttttg tggggcggta tgcatgcatt gtctagaaat
    24121 acagggacaa tttctatctt accgtttttc tttatttctg ttttgctaat gggaatttgt
    24181 attgctgttt tccaagcata ttattatgaa gaaattattt atccgcgtta tttcaagatg
    24241 acttggcgtt tggtttttct gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac
    24301 atactttctt acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt
    24361 gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg atttcccaga
    24421 aaaggtggta gaaagtgggg gattgtggta gactaatttt agttagtgga ggaatggggg
    24481 gctttcaatg ttgatgggtg aattccaaca taatattgat gcaaaaggac gtttaatcgt
    24541 gccctcaaaa cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg gattggatgg
    24601 ctgtcttttc ggctatccga tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat
    24661 gccgttggct aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg ctgccactga
    24721 atgcgaactt gataaacaag gacgaatcaa tatacccagc acgttaagaa attatgcagc
    24781 tttgacaaaa ggttgcgtag tcatcggtgt atctaaccga attgaaattt gggatgagac
    24841 acgatggcaa gatttttctg cagcagcaga agagaacttt gacgaaattg ctgaaagtat
    24901 gattgatttc ggactataag aataggaaga ggctgactaa tgactgaaac atttcaacac
    24961 tatacagtca tgttgaaaga aacggtagat ggattgaaca tcaaaccgga tggcgtttat
    25021 gtagactgta cactcggcgg tgccggacat agtgaatatt tattaagcca actaagttct
    25081 aaagggcatc tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt
    25141 ttagcttctt atgtcgaaca aggtatggtc acatttatca aagcgaactt cagagaatta
    25201 gaagaacgct tgagtgagca tgtagataag gtagacggca ttttgtatga tctaggggtt
    25261 tcctctcctc agctagatga agcagaacgt ggatttagtt atcatcaaga tgcaccgttg
    25321 gatatgcgca tggatcagtc agctccttta tccgcttatc acgtaattaa tgaatattcc
    25381 taccatgaat tagtaaagat tttcttccgc tatggcgagg agaaattttc caaacaaatt
    25441 gctcgtgaaa tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc
    25501 gaaatcatta aacaagtcat cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa
    25561 agaatttttc aagctgtgcg gattgcagta aatgacgaac tcggtgccga agaggcctct
    25621 ttagaacagg caatccggtt attgaaagtc aatggaagaa tcagcgtaat cacattccat
    25681 tcattggaag acagaattgt caaaagtatg tttaaagaat atagtacggt acaagatcta
    25741 ccaccgggac ttcctgtcat acctgacgaa ttccaaccag aactgaaagt cattactcgt
    25801 aaaccaatcc ttccttcaga ggaagagcta acagaaaaca atcgatcacg cagtgcaaag
    25861 ttacgtattg cagaaaaaat aagaatgaaa gaggagtaga atgatggcag aattaaagaa
    25921 aatgcaagat tacgaatatg atttacctga attggtggat cagccggaac aacaaatgcc
    25981 tgattcttct tcgaatcaag aaatcattct tccacaatca ccggcaaaac gattaaagca
    26041 gatttctgct ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg ttgctttatg
    26101 tgttttaaca gttatgctgc gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat
    26161 acaaacagaa atcaatgata aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga
    26221 actttctcgt actgaacgga ttaaacaaat cgcagaagaa aaaggattgt ctattaatga
    26281 cgacaattta aggaaagtga agtaaatgag tcttaaaaat aaaattcggc ggtttatgga
    26341 aaagaaaaat ctaaatccga tgaacaatcg caaaaaagta ggtatcattt tatttgctac
    26401 gagtattgga ttgttctttt tatttgccgt ccgtttctcg tatatcgtta ttggaggtca
    26461 tgttgctggt acctcattag ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt
    26521 aaaagcaaaa agaggaacga tttatgatcg taacggtgtg gcgctcgcag aagatgcctc
    26581 ctcttattca attaaagcga tactctcaaa aacatatacc tctggggata aaaaactgta
    26641 tgttgaagaa aagaattttg ataaaatagc agaaatcctg cataagaatc tttctataga
    26701 caaaaaagat gcactgaata ttttagagga cggcgcaaaa aaagagctgt atcaagttga
    26761 atttggtagc tatgggaaaa atatctccca agaaaccaag caaaacatcg aagcggacat
    26821 gaaaaaagaa ggtgtcgctg gtctttattt tgtcgaccat caggctcgga tgtatccaaa
    26881 tggcgtcttt tcttctcatt ttattggata cgcagttcca gacaaagatg agaatggctt
    26941 agttggtaaa ctaggtttgg agtctgctta caatgatatc ttgagcggta aagatggtaa
    27001 gattatttat caaaaagata attttcaaaa tcctctgcca ggtacagtgg cagaagaaga
    27061 aaaagctgtc gacggacaag atatctacac gactttagac agcagactac aaagttattt
    27121 agaaacactg atggatcaag taaatgaaga atatcagcca gaagaattga cagcagtcct
    27181 gatgaaagca aaaaccggag aaatcttggc aatgggacaa cgacctactt tcaatcctga
    27241 aacaatggaa ggattgactg gaaaagatgc tatctggcgt aatttcttgg tacaagatag
    27301 ttatgaacca ggatcaacca tgaaagtttt cactactgca gctgctatcg aagaaggaga
    27361 attcaacgag aatgaaacct tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa
    27421 tgaccatgac tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt catggtcaag
    27481 taacgtcggg atggtcatac tggaacagcg attaggcgga cgatggtata actatttaca
    27541 aaaactggga tttggacaaa gtactcattc tggattagat gatgaagtaa acggtgcttt
    27601 acctacatta aatattgtcg accgagcaat gagtgcttat ggacaagcgg taggtgtgac
    27661 gaatttccaa atgatgaaag ctttcacttc tattgcgaat aacggtacaa tgattcaacc
    27721 tcgatatatc agcaaagtgg tggatcccca aactggagaa gagcgaacaa ctcaaacaga
    27781 agttctaggg caaccttttt caaaagaaac aacagaaaaa gtccgtgaat atatgagaga
    27841 tgttgtcgaa agcgaaaact acggtagtgc ttatggtgta tatagtgtac ctggatacaa
    27901 tgtatcggca aaaaacggaa cagcccaaat cgcttctgat acagggggat atcaaacagg
    27961 agatacagcc tacctgtatt cgattgtgga gatggttcct tctgaagatc cagactatgt
    28021 cttgtatcta acaatgaaac accctaagac atatgatcga atggcattgg ctaaaatcgc
    28081 aaatccattg atgaaacggg caatggattt taaagaaact gaagaagatt cagatacaga
    28141 aacaaaaaca gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc
    28201 agctgatgct caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca
    28261 aaaacaatct actgccaacg gtgatcagtt gatctctgga gaaaaactga tcctatatac
    28321 tggtggtgat aagctgatgc ctgacgtaac aggatggtca aaagcagata tcatgaagct
    28381 ggggaaaatc ctcggtattg aagttagttt tgatggagat ggttattgtg tcaaacaaga
    28441 acttgctcca tatgaaaaaa tcactgaaga caaacttagt tttactttag aagaataaga
    28501 agagaaatag gagagaaaag agcatggaat ggacacaagc attgattccc attgtcagca
    28561 gttgtgcaat gacaattgcc gcaatgccgc tatttattgg ttattttcaa atgaaaaagc
    28621 aaggtcaggc gattcgtgag gagggaccaa agtggcacaa tagcaaagcc ggaactccta
    28681 caatgggcgg cttagtgttt ctgatcggct ctattttgac aggaatttgg gttggagctt
    28741 ggcaaaaaca gctaactcct actcttttca tcttgctttt tgtattagcg ttatacggag
    28801 taatcggctt tcttgatgat tttataaaaa tattcaagaa aagaaacatg ggactgaact
    28861 cgaagcagaa attgattgga cagattctag ggggaatcat cttttatctt gtttaccgtt
    28921 ctgaaggtta tccaggtgta ttgaatttct tcggactaga tctaccttta ggaatcattt
    28981 atggtatttt cgctctcttc tggcttgttg gattttcaaa tgcagtaaat ctgacagatg
    29041 gcatcgatgg attagttgct ggcttaggaa gtatctcttt tgcagcttac ggtctaattg
    29101 cttggcatca gcatcagtat gatgtattaa tcatttgctt aagtgtttta ggaggtttac
    29161 tgggtttctt cgtctacaac agaaaacctg ccaagatttt tatgggagat gtcggttcat
    29221 tagcgttggg cggactttta gcggcaattt ctataatgct aaaccaagaa tggacattgc
    29281 ttttgattgg tttgatctat gttatggaaa cagcaagtgt gatgcttcaa gttacttcct
    29341 ttaagcttac aggaaaacga atttttaaaa tgtctccaat tcatcatcac tttgaaatgt
    29401 gcggatggtc ggagtggaag attgatacag ttttttggtt gatcagcatt gtgacttcgt
    29461 tgatcacatt atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat
    29521 gaaaataaga aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta
    29581 ttacatgaat tgggcgcact tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg
    29641 gaagcacaag agctgctttc tttagggata aaagtaataa caggtagtca tccaatcgaa
    29701 ttgttggatg aagaattttc attgatggtc aaaaatccag ggattcccta cagtcatcct
    29761 ttagtagcaa aagcccaaga aatgggtatt ccggtaatta cggaagttga gctagcttac
    29821 gaagtagcgg aatgcccaat tattgggatt acaggaacga acggaaaaac aacgacaaca
    29881 acgatgacag gactactact gaatgcagga gcagatcaag gaattgcacg tttagcgggt
    29941 aatattgggt atccagcaag tggtgtagcg caagaagcaa aatcagaaga taaaattgtg
    30001 atggaattgt ctagcttcca actcatggga atcacagatt tccgccctca tatagcggtg
    30061 attaccaata tctatgaagc acatattgat tatcatggta caagaaaaga atatgtcaaa
    30121 gctaaatgga accttcaaaa aaacatgaca gaaaaagatt atctgatcct taactggaat
    30181 cagtcagaac tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca
    30241 aaagaagtgt tggaagacgg tgtgtatgct gacgattact caatatacta taaaaaagaa
    30301 aaaataatgg aaatatctga acttggcgta ccaggtaagc ataatgtaga aaatgcacta
    30361 gctgctattt ctgtagcaaa attgtatggt atatcaaatg aagctatacg agaaacgttg
    30421 cattttttcc atggcgtacc acatcgtacg caatatgtag gagaaatcca aggcagaaaa
    30481 ttttataatg attcaaaagc aaccaatatt ttagcgacta aaatggctct aagtggattt
    30541 gaaacgtcta aggtcgtgtt attggctggc ggattagaca gaggaaatac atttgatgaa
    30601 ttaattcctt cattaaaagg aatcaaagca atggtcgtat ttggccaaac aaaagaaaag
    30661 ttgatggatg ctggaaaaaa agcaggcatc gagacaatag taacagccga ttctgtagaa
    30721 caagcagttc ctttagcatt agaaaacagt acagatggtg atgtagtttt attatcacca
    30781 gcgaatgcca gctgggatca atacccaaat tttgaaacta gaggaaacag attcatggaa
    30841 gctgtgaacc gattaaaata gaaaaagtag aagcgagcga taaaaaatga aaattttggt
    30901 tacaggcgga ggaacagggg gacatattta tccagctctt gcctttgtca attatgtaaa
    30961 gactcaagaa ccgaatgctg aattcatgta tgtgggagca aaacgaggtc tagaaaacaa
    31021 aatcgtccct gatacaggaa tgcctttcca taccttggag attcaaggat ttaaacgaaa
    31081 aatttccatg cataacatca agacggtccg attgtttctt aagagtatcc gtcaagcaaa
    31141 aaaaatcctc agtgattttc aaccagatat cgttatcggt actggaggat atgtttccgg
    31201 agcggtagtg tacgctgcag ccaaaatggg tattccaaca gttatccatg aacaaaacag
    31261 tgtaccaggt gttaccaata aatttttaag ccgatatgtg gatcggatcg ctctttcatt
    31321 taaagatgcg gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc
    31381 acaagaagta gcagatacta aaaaatcaga tattttgaaa acatttgatt tggatccaga
    31441 aaagaaaaca gtcttgattt ttggcggtag tcaaggagca cttaaaatca atcaagcagt
    31501 atcagttttt ttaagtgaat atattccttc ttcttaccaa gttttgtatg cttcaggaga
    31561 tagatattat gaagagatcc taaataaaat aaatcctgaa atacaaaata ttagtgtcca
    31621 gccttatatc aaaaatatgg cagaggtcat ggcttccagt gatctgctgg tggggcgtgc
    31681 tggtgcaact tcgatcgcag aattcacagc tctggggtta ccggcagtgt tggttcccag
    31741 cccatatgtt acgaatgatc atcagactaa aaatgcaatg agtcttgttc acgcaggagc
    31801 agcaaaaatg attgcagata atgaactaac aggtgaatca ctaagtcaga cagtcaacga
    31861 aatcatgggt gatgaagaat tgcaaaagca aatgtgccga gcttctaaag aacaaggaat
    31921 ccctgatgct tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa aataaaggcg
    31981 gtggtcttag tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa
    32041 aacaatttga ctccttggca aaaggctaac cgaaaatatc tggcagaaca agagatgaaa
    32101 aaccaagagc caaaaaaaga agacataaca ttagatacac ccctttcaga ttctgaagaa
    32161 gaaaaaacgg ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt
    32221 tttgaagaga tcaagcaaaa gaaaaacggt ggaccatata acggttcttt tttgaaccgg
    32281 ctgcccaatt taaaaagtca acggcacaaa gtactatttc gtcggttagc tttgatcatc
    32341 actgtattgg gtataccgct gatttttttg atctattatg tttctcctta tagcaaatta
    32401 caagcagttg cagtatcagg caataagaca gtcaacagcc aagaaatcat ctctgataca
    32461 aaattgtcat taggagaaaa tgtttgggga cagtattttc atcgatctac ttatatcgat
    32521 cgccttaaaa aagcacagcc aagaatcgaa acagcaaata tacatttcaa agggatgaat
    32581 gaattcgatt tggatgtaac agaatataaa gagattgctt tgatcgcaaa aaacaatcaa
    32641 tactacccag tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa
    32701 aatctcccta tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa
    32761 tacaatcagt tatcatcaga attacaaaaa gcaatttctg aaatcaagta cacgccaaga
    32821 gcaagcaata aaaatctgat ccaattgaat atgaatgacg gcaatcaagt tattgtaaat
    32881 atcaataatc ttgcgaacca aatgaaatat tattcgcaag tagccaaaga tatggatgaa
    32941 aaaggtgtga tcgacatgga agttggtatc ttctcgcatc cttacggtag cactcaagaa
    33001 acggaagaat cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca
    33061 aacgaatcaa gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc gtctagcaca
    33121 aacggatcat ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga taatccaagt
    33181 acaactccgt caagtttaaa tgaaaattga taaaacaact ttaaaaagta agaatgtact
    33241 ttctaaaaac cagtaattta tggtagaatt ggaagcagtg aatatatcta ttaataagta
    33301 tattttaaaa cggacgctag ttgttcgctt gtatatggaa tagtaagtag gagggaatcc
    33361 cattcatggc aaaaacagga atgtatgtag gccttgatat tggaacgaca tctgttaaag
    33421 ttgtcgtggc tgaatatatc gacagccaaa tgaatattat tggagtagga aacgcaaaat
    33481 cagaagggat taaccgaggc attatcgttg atatcgataa aacggtccaa gcgatacaac
    33541 gagcagtacg acaagcagaa gaaaaagcag gtatccagat caaaggcgta agtgtcggtt
    33601 tgccagcaaa catgcttgaa gtagaaaatt gccagggaat gattgcagtt aacggagatt
    33661 caaaggaaat caccgatgaa gatgtcagaa atgtggcttc agctgccctt gttcgttcaa
    33721 tacctcctga acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc gatgggttcg
    33781 agggaatcaa agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt
    33841 ttacaggacc taaaacgatc atccataaca tacgtaaatg cgtggaaaat gccggattaa
    33901 tcgtcaacga aatggtgatt actcctttag cgctgactga atcaattctt tctgacggag
    33961 aaaaagattt tggtacgatc gtcattgata tgggtggcgg ccaaacaaca acggctgtta
    34021 tgcatgataa gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta
    34081 aagatatctc tattgtatta aatacgtcat ttaacaatgc agaagcgttg aaaataaact
    34141 acggggatgc atacccagaa cggacatcac cagatgaaga atttccagta gatgtgattg
    34201 gtcagtcaga accagtgaaa gtcgatgaac gctacttgtc agaaattatt tcagctcgaa
    34261 tggaacagat tttcaacaaa gcaaaagatg cattggacca aatcgaagca ttagagttgc
    34321 caggtggcgt cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg gacttggcac
    34381 aagaaatctt cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc
    34441 cggtatttac aaatgtaata agtatcgtgg attattcagc aaacttgagt gaagtgtatc
    34501 aattggcaaa aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac
    34561 aagaaacaac aaacacatat gagagctatg aagcacctga agaaacaatc tacgatgagc
    34621 cggaaccaaa agagtccgga gaaaatgtaa aaaataaaat caagggtttc tttacaaata
    34681 tttttgatta attcatcctg ggaggaataa gatatggaat tctcaattga taataatatc
    34741 aatgacggcg cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac
    34801 cgaatgattg aagaaaacgt taaaggtgtt gaattcatta cagccaacac agacgtgcaa
    34861 gcattaaaaa attcaaaagc tgaaacagtt atccaattag gccccaaata tactcgtggt
    34921 ttaggtgctg gttctcagcc agaagttgga caaaaagcgg cagaagaaag tgaacaatcc
    34981 ttacgcgaag ctttagacgg tgcagatatg attttcatca ctgcaggaat gggtggagga
    35041 accggtacag gtgctgcacc aatcgttgca ggtatcgcga aagaattggg cgcattgact
    35101 gttggtgtcg tgacacgtcc attcactttt gaaggaccga aacgtggccg ttttgctgca
    35161 gaaggtatcg ctcgtttgaa agaaaatgtg gatacattat taattatctc aaataaccgt
    35221 ttattagaag ttgtagataa aaagacaccg atgcttgaag ctttccgtga agcagataat
    35281 gttttacgac aaggtgtaca aggtatctct gatttgatca cagcgccagg ttacgttaac
    35341 cttgactttg ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt gatggggatc
    35401 ggtgtggcta gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca
    35461 ttgcttgaaa catctattga tggagctgaa caagtacttc ttaacatcac aggcggtttg
    35521 gatatgacat tgttcgaagc acaagatgct tcagatatcg tagcaaatgc agcaactggc
    35581 gatgtaaata tcattctagg tacatctatc aatgaagaaa tgggcgatga aatccgtgtg
    35641 acggtcatcg caacaggtat cgatgaatca aaaaaggaac gcaagtctag ccgaccagct
    35701 agacaagcac aaatgcaatc acctgctcaa aagactgtat tagatatgga tcaagctaaa
    35761 ccaacttctt cagaagaaga aaacagtttt ggcgattggg atatcagacg tgaacaaaat
    35821 gttcgtccta gagttgatga ttccaatttt gacaatatcg aaaaaaaaga atttgataca
    35881 ttcaaccgcg aagaaacaaa atctcatggc gatgatgaat taaatacgcc accgtttttc
    35941 cgtcgtaaaa aataaaggag aggaagagca catgattgct gacaacttgc agaaaatcaa
    36001 gcaagaaatt caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt
    36061 agctgtaaca aaatctgttg aatccgctca agctgaagaa ttggttagag ctggcgttat
    36121 gaattgtgca gaaaaccgag tagataaatt attagaaaag aaacaagatt tatttgattt
    36181 tcctgaaata aagtggcatt taattggtaa tttacagcgc agaaaggtaa aattgattgt
    36241 aaatgaaatt gattactttc atgcgttgga cagtttaaga ttagcagaag aaatccagaa
    36301 gcggttagag aaagaattat cctgttttat tgaagtaaat gtttctggtg aggaatcaaa
    36361 acatggaatt tcaccagatg agcttttacc gtttgtggaa tctttagctg cctgcacaaa
    36421 aatcaaggtt gttgggttga tgactatggc gcctaaagaa gcttctgatg agcaaatcca
    36481 taaagtgttt ggtacgctga aagaattgcg tgatcttgtt caagcaaaag gttatgccca
    36541 tgcaccatgt agtgaattaa gtatggggat gagtcaagac tttcaaattg cgattgaaga
    36601 aggtgcaaca tttatccgtg taggaacagc gctattcaaa gacgaataaa ggaggaacaa
    36661 aaatgccgct tttaaacaga ggagcaattg ctaatttttt cggattagct gatgaggaag
    36721 agtacgaata ccaagattat cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa
    36781 atacaacaca gaatcaatca actcattctg tatctgctgc gaatactcat gaaaaacaaa
    36841 aaacggcgac ttcacgtcct gtaacgcatg aaagtattca gccggctgaa aagaaaaaag
    36901 taattgaact acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa
    36961 cctctacaca acctaaagca aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt
    37021 attctgaagc tatggcgatt gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt
    37081 ttcatttagt agaagaaaaa caagcgagaa gaatcgttga ttttctaaca ggaacagttt
    37141 atgcactaga tggagatatc aaacgtgttg gagatgagat ttttctttgt acaccgactg
    37201 ggatggagat tgacagttca accgctcaat cttttgcaga tgcgaatttg tttgatctgt
    37261 aagaggagga actacgattt atactattgc actattactt catttacttc aacgggcagc
    37321 ctatttctat agcattatct tgatggtata tgcattgctc tcttggtttc caggcgggta
    37381 tcaatcaggg ctaggacgat tattagctaa gatctgcgaa ccgtatatca gtttgttcga
    37441 tcgtctgcct ttgaggattg gacccttgga tttcacaatt ttaggggcaa tcctgatctt
    37501 gaacttagcc gtacaaggtt tagcacagat cgttatatgg ataactgtcc atttagttta
    37561 ttgattgaag caacaaaatc tttattctca gaatagatag gaggggaaaa ttttggatgc
    37621 aaatgtatac cagcactttc gggcagatga acgtacattt atcgattcgg taggcgattg
    37681 gattgaacag gttcaaggac aatatgcacc ctatctgacc gaatttcttg acccaagaca
    37741 atcctatatt ttagaaacac tgattcgaca aagcagtgat ttgcgtttca tgttttatgg
    37801 cggatatgaa caggcagaaa gaaaaagatg tttgattttc cccgattatt acgaacctgt
    37861 agaggaagat tttgaaacag cattgtatga aatccattat cctagcaaat ttgccagcct
    37921 aagtcatggg aaaatccttg gaactttagt gggaacgggg atcaaacgct cttactttgg
    37981 cgatatcatc tcggacggtg atcgctggca agtatttatt gcaaatgagg tggatcattt
    38041 tgttgctgca caagtaacaa agattggaaa ggttgctgta cgcttggaac caatcaaata
    38101 cacagaaatg attatgccga aagatagctg gacacaagaa agaggaacag ttagttccat
    38161 gcgactagac agtgtgatct ctgagattta taatatttcc agacagcggt ccaagcagct
    38221 gatcgaagct ggtaaagtaa aagtgaactg gacagaaaat acaagacctg attttgtact
    38281 tgaactatta gatattattt cagtcagagg ttatggcaga gtccagatcc aagaacttga
    38341 ggggaagacg aaaaaagaaa agtacagggt actgttcggt gtattacgta aataaaataa
    38401 aaaggtggtt tactcatggc tttaactcca ttagatatcc aaaataagac attcccgaca
    38461 aaaatgcggg gatataatca agacgaagta gatgatttct tagatttagt tgttcgtgac
    38521 tatgaggaat tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa
    38581 aaacttgagt acttcaatga attaaaagat gctttgaacc aatcaatcat cgtagctcaa
    38641 gatacggctg ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat cgttacttct
    38701 gcacaaaaca aagcagatga gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca
    38761 actgatgcag aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa agcaagacag
    38821 cttgcgacag aaactgaaga tcttaagaaa aaaacacgag tattccatca acgtatcagc
    38881 ttgatgcttg agtctcaatt agaacaagtg aaaagtccgg aatgggatga aattttacaa
    38941 ccattctcca gctacgtgac tgacagccat gaagtgatca aagaagtttt agcaaaacag
    39001 cttgacaatg aaaatgatac tgatgtaaac tcagacacag gagtcataga agcacctatg
    39061 acggcatttg atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag
    39121 tagaacagaa aagcactttg ttattcttaa agcgagccga tgagagtgga agttcggtaa
    39181 gacactgcat ggtgaaagat cctctacttg agacctgctt ttccgaaatt agagtaagaa
    39241 gcagcggctg acctcgttat tggtcacaga gagtttgcac tagatagatg caactaaatt
    39301 tgggtggtac cacggcatgt tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa
    39361 acaaataaag gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca
    39421 aaattcccta tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa
    39481 gaaaatcgaa tctaccaaaa aagacaagag ttgaacgaag gaaaacctag ctttgttctt
    39541 catgatggac ctccatatgc caatggtaat atccatttag gacattcatt gaataaaatc
    39601 agtaaagaca ttatcattcg ttcaaaatca atgtctggct atcgtgcacc atatgttcca
    39661 ggatgggata cacatggatt gccgattgaa caagtattgg ctaataaagg cgtgaaacgt
    39721 aaagaaatgt ccatggcaga atacttggaa aaatgtcgtg aatatgcact ttcccaagta
    39781 gataagcaac gagcagattt caaacgccta ggagttgcag gagattggga acatccttat
    39841 gttacattag atccttctta tgaagctgca caagtacgcg tatttggtaa aatggctgaa
    39901 aaaggctata tttacaaagg attgaaacca atttactggt ctccgtcaag tgagtcttct
    39961 ttagcggaag ctgaaattga atataaagat gtaaaatctc catctatttt tgtagctttc
    40021 aatgtcaaag acggcaaaga tattcttgat gaagatacag cttttgtcat ttggacgaca
    40081 acaccatgga cattgccagc aaaccaaggt attgctgtaa atccaaaatt tacgtatgta
    40141 ttggtggaag ccgatggccg taagtttgtc gtagcgaaag atttgctaga aacagtccaa
    40201 tcagaaatcg gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa
    40261 tatatgactg caagtcatcc attctatgaa cgtgaatcac ttgtgatttt aggcgatcat
    40321 gttactttag acgctggtac tggattagtc catactgctc caggacatgg ggaagatgac
    40381 tatatcgctg gtagcaaata taaactatct gtagtgtcac ctgtagactc aaaaggattg
    40441 tttacagaag aagcacctgg atttgaaggt atcttttatg acaaagcaaa tcctatgatc
    40501 actgatctat taaaagaaaa aggagctttg ctgaagctag atttctttac tcatagttat
    40561 cctcacgatt ggcggacgaa aaagcctgtg atctatcggg caactccaca atggtttgca
    40621 tctatcgata aattccgtca gaatattttg gatgaagtag aaaaagtaga gtgggttatc
    40681 ccatggggta aaacacgttt gtataatatg atccgtgacc gcggcgattg ggtaatttcc
    40741 cgtcagcgtg catggggagt accacttccg attttctatg ctgaaaatgg agaagcaatc
    40801 atcacaccag aaacaatcga acatgtggca aaattatttg ctgaacatgg atcaattatc
    40861 tggtttgaaa aagaagcgaa agaacttctt cctgaaggat ttacccaccc aggttctcct
    40921 aatggcgaat ttacaaaaga aaaagatatc atggatgtct ggtttgattc tggttcttct
    40981 catgaagctg ttttacgtca gcgaccagaa ttatcattcc cagcagatat gtatttagaa
    41041 ggatcagacc aatatcgtgg ctggttcaac tcaagtatca cgactagtgt ggctatcaat
    41101 ggtgtagcac catacaaatc agttatttct caaggatttg ctttagatgg tgaaggccgt
    41161 aagatgagta aatctttagg aaacatcatt gcaccagaaa aagtgatcaa acaatttggt
    41221 gcagatatcc ttcgcttatg ggtatctagt gtagattatg aagcagatgt tcgtgtttct
    41281 atggatatct taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc
    41341 ttattagcga atacagaaga tttcgatcct aaaaaagata ctgtttcgta caatgattta
    41401 cgttctgtag acaaatatat gaccgttcga ttgaaccaag taatcaaaga aatccgagaa
    41461 gaagggtacg acaaatataa cttcatgcat atttaccgta cagttatgaa cttcttgacg
    41521 gttgatctat cgtcattcta cctggatttt gctaaagatg tagtttatat tgaagcagaa
    41581 gatgattatc aacgtcgttg catgcaaact gttttctatc aaacagctgt agctttgaca
    41641 aaattattga ctcctatcat tccgcataca gcagaagaaa tctggtcatt cctgaaagaa
    41701 gaagaggagt acgttcagtt aagcgagttt cctggttatg aagagtttgc aaaccaagaa
    41761 gaattgatgg atacctggac tgctttcatg gacttccgtg ataatgtctt aaaagcattg
    41821 gaagaagcaa gaaacagcaa attgattggt aaatctttgg aagcaaaact aactgtttat
    41881 ccaaaacaac aagttcgtga aatgttaaaa gctttggatg cagatattgc acaattattg
    41941 atcgtttcac cagactactt tgaagttatg gcagcagatg aacaagtgcc agataacgct
    42001 atggtatttg atgatgttgc tattacagtg gaaaaagcag acggggaaac ttgcgatcgc
    42061 tgtcgccaag ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt
    42121 tgtgcaaaaa tcgttgaaga cttctatcct gaagctgtag ctgaaggatt cgaagaaaag
    42181 taaaagggaa aagggggtgt gacatgttct ttgtcacacc cccttttgtt atcaatcgtt
    42241 taattttcca cgttcacgat accagttatc tggttgccag atccattcga atccgtcttt
    42301 ttcaagtagt tcgaaagctg ctttaggtcc cattgatcca gctttgtagt tgggaaaatc
    42361 cggttgtgtt tggtcccaag cttttcgaat gacatccacg atatgccaag attgagcgac
    42421 ttcttcccaa tgagagaagt tcgttccatc accattcaac gcatctaata gtaatttttc
    42481 gtaagcttct gggctatttt caaccatttc tgcactattt ctaaattcca atttgattgg
    42541 ttccgtttca aatccttgcc ctacttcttt cccattcaag gataaagaaa atccttcagt
    42601 tggctgaata tagatcgtta gaacattagg tggaagtgtc gtatcgtcac atggttcatc
    42661 gacagatgtt ttgaatacat taacaggaac ttgtttgaat acgatgttga tccgagtccc
    42721 tttttcagtc aaacgtttgc ctgtacgtac atagaaagga acacctgacc aacggaagtt
    42781 atcgatagtg aatttgcctg cgacgaatgt ttcagtctct gattgagcat ccactttgtc
    42841 ttcttcgcga tagcccttga attgtacacc atccaattca ccttgagcat attgcccgcg
    42901 aacgaagttt tctaacactt cttcttcgtt ataaatgcgg atagctttca atgctttgat
    42961 cttttctgta cggatttctt tttcggaaaa tgctactggc ggttccattg caagtaatgc
    43021 tacaacctgt aaaatatgat tttgcaccat gtctttaagt gcgccactat gatcgtagta
    43081 gcccccgcga tcttctacac cgaggttttc gccaaaagta atttggatat tgtcgatata
    43141 gcgattgttc cattgagcct caaaaatatt attggcaaaa cggatagctg agatgttttg
    43201 gatcatttct tttcctaaat aatgatcaat cctgaaaata tcttgttctg ggaatacttc
    43261 tctgatttgc tggttcaatt caaaagcaga ctggtaatca gaaccgaacg gtttttctat
    43321 gatcaaacga ttgaagccat tttttgtcat aatattttgt gatttcaaat gagaaacgat
    43381 cgttccaaaa aattgaggtg ccatagccaa ataatagaca cggttaccgt ttaattgata
    43441 gtgtccatct aatcggtcag acaattcttt taatgtctga taatgcgttg aatcatttac
    43501 atcatgggat tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt
    43561 caatgcacgg attgtttctt tgacaacttc acggtagtgc tcatcgctcc agggacggcg
    43621 agcggttccg attacagcaa aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa
    43681 agacgggtac aatttacgct gggctaggtc acctgtaccg ccgaatatcg taaacaatac
    43741 atttttttca ttagacatgt ggatacactc ctttatattt tatcgacaaa aatcgtgctg
    43801 gccgccttaa agctgacggg gattttcttt ctatcatttt ggatagtgat cggtccttca
    43861 taagctgcga tgtcaacgat ctcatattct tcgttgatat tcaggtcaat agtgactaag
    43921 taatccaaca attctttttc atcaagcact cgtgcgatac gcacttttgt accgacagga
    43981 taggactcca atgtttgtcg gcgttcttca tggacaactt cctctttttt aggaatcgat
    44041 cctccatgag gacaatgttc aggatgattc aaatagtttt ctaaatgatc tgcaagatgt
    44101 tccgaagtca catgttccaa tacttcagca tcatcatgga cttcattcca gctatagtct
    44161 aaatgttcaa ctaaaaaaac ttcccataag cgatgtttgc gaatcagact gctagctttt
    44221 tgcagtccgc ttgcagtcaa ttgtacacct tgataagggg aatgttctac aagctcttct
    44281 ttcacaagtt tagaaatcat ttcactgaca gatgcagctg agacatctag actagatacg
    44341 atttgtttat tgttgacttt cgttgtatct ccacctagtt ctaaaatgat tttcaaataa
    44401 tcttctcgat ttggtgtcat attcgatcat cctttcatgg taaaaaacca tcattagtaa
    44461 gtgtaacaaa aaaaacgtta attgactatt tttctaaata aaaaacaaag gatttttaga
    44521 cgtttttaca acaaaaaacg aaagatgata gagaagactc attctttcgt ttataaagga
    44581 tatatatata cattgggagg gatctatttt tcagataaca acttatatga agtgtctgca
    44641 gattcgtcat aaagagtttg ttcgggataa gaaaagataa gataaaaacc atactgatca
    44701 cgtacgacta aacgatattc atctatttct accacagatg tgccaagaga ctgaccgttg
    44761 atcaaaaacg tgtaatcaga tgtaatctgt aattgttgtt tttgctggct atcactaaag
    44821 aaccatgtac cttgatactt tgagagtcct tctgactttt ccgcttgttt tttctttttc
    44881 aagtaaataa aacttgagag aacggtaatg agtgaaccaa gaaaatagat catttttttt
    44941 atcaccaaat cactccttta tgagaaaata atcgcataga catattcttt cttctttatt
    45001 tcattgtacc gaataaaagc taaaatttct ctaagacttt cgactggttt ttttactgtt
    45061 tttctacaaa ttatgatagg atacatagta ggaagaaagg tgacataatt tgaagtatca
    45121 gcaagtggaa atagcttttt ttattgaccg cccgaatcgg tttatagcac attgtatgaa
    45181 aaaagacgga gaaataatta ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct
    45241 gataccaggc gctgaagtgg ctattgtatt tgctcctgga cctaaacgga aaaccgccta
    45301 tgatctgatt gctgtaaaaa agaagggtga ctggtttaat atcgatagtc aattgcctaa
    45361 tcggctggca attgatggta ttttagacgg cacgatcctt ttaccaggat taactggtcc
    45421 atttacggta tacaagcgtg aagtaactta tcttcactcc aaatttgata tttatttaga
    45481 gacggtggtc ggacaaaaag catttgttga agtcaaaggt atgacacttg aaaataaagc
    45541 gattggtgct tttcctgatg cgcccacact cagaggattg aaacatatag gagaattaac
    45601 gtatgccgca caagatggtt atgccgctta tgtcttgttt attgctcagt tcgaacatct
    45661 tcatctggca acgatccatg aagaaatgca accagcacta gctgatatgg ttcgacatgc
    45721 gcagcaaagc ggagttcaaa tcctcgccta taattgtcaa gtatcggcag atgaggtaac
    45781 tgtcaagcag gccattccat ttgatgtaga tatccctttt gaagatccta tacaatagaa
    45841 agaaggtaaa ataatgatca acttaggtat tatcggaaca aattggatta ctcatcaatt
    45901 tgtccaagca gctttaagca caaaaaaata tgaattgaca gcggtatatt ctagacattt
    45961 ggaaaaagca caggaatttg gttctcatta tgaaggggat atcgaattcg caacagattt
    46021 gaagactttt ttcaatatta ctcatatgga caccatttat attgcttctc cgaatagtct
    46081 tcactttagt caagctaagc aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc
    46141 agctttttcg acgccaaaag aaatggatga aatcatccat ttggcaaatg aacacaatgt
    46201 gtattttttt gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta
    46261 cttaccatta aaagacgaga taatcggtgc aaatttcagc tatatgaaat attcttcgcg
    46321 ctatgaccaa gtattagatg gagaagaacc gaatatcttc tcgactcatt tctcaggtgg
    46381 tgcattgatg gatttaggtg tttaccttgt atatgcagct gttgcttggt ttggaatgcc
    46441 aaaagaagtc cattatttcc caagaaagat ttctactggt gtagatggtc ttggatgggg
    46501 gatcttacgt tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg gtgattctta
    46561 tttaccgtca gagatttatt ttgactcagg aacgctagtg atgaatggag tcaatgcaat
    46621 tgaacaagca caatttcatg atcgcgcgca taaagaaatc attgatttgg cggtaacagc
    46681 tgcagaaaat ccaatggcag atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa
    46741 agatccagct tggggattgt tgtatgaaga gtggatcgag ctagctagaa atgtgaacca
    46801 agtgatttat gatttaagaa aaaatggcgg catcaaattc gatgcagata atgaggaaga
    46861 tttttaatga acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa atatgaggat
    46921 aaagggttta tctcaccttc tcttattcaa cgagcagttt ttcagccact aagtaataaa
    46981 caaagtatag tagcggtttc tcctacaggt tcaggtaaaa cactggctta tctttggcct
    47041 ctactactaa acgtggaacc aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa
    47101 gaactagcta ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa agagttgaaa
    47161 gtccaatctt tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa
    47221 ccagaaatct tgattggtac accaggaaga atattggaat tgatgaaagc aaaaaaaata
    47281 aaagcacatc aagtgaaaac aatggtattt gatgaagcag accagctatt tgacgctggg
    47341 aacagccaaa tcattgatca aattcttcat caagctccaa cagagtatca gctagctttt
    47401 ttttcagcga cagcagatcg ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt
    47461 gagacaattg acgtgacagc agaggacgat tctcgaaaag gactgagaca ttacttttta
    47521 cgtgtaccga tacgaaagaa agatgactac ttgagacgtc tcacgcatat agaagatttt
    47581 catggtctga ttttctttaa tcaactgact gaattaggcg taatggaaga aaaattgctg
    47641 tatagaggtg taccggtagg aagtctggcc tctgatcaga acaagctact ccgaaaagca
    47701 gcgctagaac aattcaaaaa gaaaatggtc agagcacttt tgaccacaga tgtggcggct
    47761 agaggtttag atattacagg tcttccttat gtagtgaatg cagaagttcc gttaagtgaa
    47821 gaagcttatt tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt
    47881 acactcgtac aagataataa tctaagagat ttaaaacggc ttttacgtcc atcaaatata
    47941 acacttgaag agattttttt atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa
    48001 gatgcagctc ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa ggagcagaac
    48061 ccagaatata acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga
    48121 aataaaggag caaggcgcaa ataagcgcct ttttatgcta tgattatgaa aacgggaaca
    48181 aaattggttg ccagaggagg ggttatgtga aagaacgtca agaagagatc ttgcagctat
    48241 tgacagaaaa ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta actatcgatc
    48301 gaagcaatgc aagccgatat ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg
    48361 ctggccgacc agtcgtttac tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg
    48421 tcgactcttc tacgcaagtg acttttgaaa cactcgttgg agaaaatgat tctttaaaag
    48481 tcagcataca gcaggcaaaa gctgcgattc tttatccacc gcgggggttg catacgatta
    48541 tttttggaga aactggtaca gggaagtcga tgtttgctga atgtatgtat cattttgcaa
    48601 tcgattcaga gatgctttct gcagatgcgc catttgtttc tttcaattgt gcggattatg
    48661 atcaaaatcc gcaactgctc tttggacaca tctttgggat caaaaaaggg gcttacacag
    48721 gagcagctca agatagtccc ggtctaatag ccaaagcaga cggcggtata ttgtttttag
    48781 atgagatcca ccgtttaccg ccggaagggc aggaaatgct gtttactttc attgataaag
    48841 gtatttaccg tccgcttgga gaaagtgcac aagtacatga agcatctgtt cagatcattg
    48901 gggcgacgac tgaatcttca gaaagtttct tatctacgtt caatcgacgg attcctatgg
    48961 caatcacctt gccgaatctt gcttcacgtt cactggatga gcgttatgaa atcatctctc
    49021 tttttatcaa acaagaagcg aatcgtttga atcaacgtat cgatatcgaa aaagaagcga
    49081 ttcttgcctt catgctttat gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga
    49141 aactagtctg tgcaaaatct tttttacatt accgtactca tcatgagaaa aagctaatca
    49201 ttcgaaaaga agaactttcc cttcaagtac aaaaaggatt actgaaaatc aaggaaatac
    49261 cggaacgtct cgaccgattc atggacagca aaagtcagta tttgacattt gaaccaggat
    49321 ttgctgatgt tgtctggtca caagatccag aacgaaacat gcaggtctat aacgatattg
    49381 aggaaaaagt cacttctctt tccgaaaccg gtgtggagaa cattgatcta gagaccctca
    49441 tttcaaaaga tgtggatgct tatttccaga catatgtcaa agaattgact aaaagttcga
    49501 ttcccaaaga tcttttgcca gatgaaatct ggcggttaac caatcaattg tatgatgtgg
    49561 ctgaaaaacg gttagaccgt atttataatg aaaaagctcg ttttgctttt gctttgcatc
    49621 tgcaaagtac attggatcgt gtgagagaag gtcatatgat cgtccaccca gatttgaata
    49681 acgtacggaa aaattttaag agggaattcc aggtagcaat cgatctttca acgatcattg
    49741 aagaagaaga acaagttgag attccttttg atgaaatcgg ctttattagt atgtttcttt
    49801 ctatcaatct aggagaaagt gagcctgtta aagaaaacaa agtagatgtt ctcatattga
    49861 tgcacggcag acagactgct tcaagcatgt tggagatggc acaagaacta ttaggaacaa
    49921 aagtagggaa agcttttaat atgccactgg ttatggaagt tcaaacaatg tatgaagaag
    49981 tgttacaata tgtcaaaaac tatcaagagc atttgacgaa tggcctactc ttgttaacag
    50041 atatgggatc attaaacacc ttcagtgatt tgatttatga agagactggc attcggacga
    50101 aagctatcac gatgacaagt accatgattg tgatagaagc gatacggatg gcaagtgttg
    50161 gaagaagctt agaagatatt tatcaaaata tccagctttc ctttgaatcg atcgtacgtg
    50221 atcagttcaa agcggaaaag tctgaaactt tacagcgtaa aaaagccgta atcgtgacat
    50281 gctttactgg tgaaggagta gctgccaaat tatatcagcg tattatgcca gtgattaatc
    50341 aaaataaggt agaactgatc cagatgcagt ttttagaacg tgaatcattc aaaaaacaca
    50401 tcgatgcctt attggaagaa tatgagatca aagcaattgc gggaacagtt gatgttgatt
    50461 atcaaaatat cccatttttt tcagcctatg acgtttttga tgatgaaaaa ctaaatattt
    50521 taaagcgtat agctgatgat gagatcccga cagctaaaat tgtcgattca cttgctggat
    50581 cattaagcga tgtcttttct attgatgaac tgatccgttc attgcaaaag acagttcagc
    50641 agatccagac cgatttgtcg attatcgtgg aacctggagt agatactgga atcgttatcc
    50701 atttagcctt catgttagac ggattgatca aacaagaaag ccatcggcaa tttgagaaac
    50761 tgtctatttt ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca aacttgatgg
    50821 cactagaacg aaaatatcag gtaaaaattc cagaagatga tatcgcattt cttacacaaa
    50881 tgtttcttga aaataaaata aaaaaatgaa cacactaatc atactgttta cacactaaac
    50941 atctgtgtgc gcactataaa aaagagctaa aaagcatgaa aacgctattt cagctctttt
    51001 ttttattggc atgcttcttg cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa
    51061 atggcaaaaa aaacaatcat gttagtttgt tcagcaggga tgagtacgag tttattggtc
    51121 acaaaaatgc aaaaagcagc agaagaaaaa gggatggaag ccgatatttt tgcggtctct
    51181 gcctcagatg cagataataa tttagaatct aagaatgtcg atgtattatt attaggtcct
    51241 caagtacgtt tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg tattccatta
    51301 gatgtcatca atatgcaaga ctatggcatg atgaacggag aaaaagttct tgcacaagca
    51361 gaaaatctaa tgaaataaat caagtatgca gttgggggta taacaacgtg gaagatcaac
    51421 aaaatctaga agccatcatg gggttaatca tgtttggcgg taatgcaaag agcgatgcaa
    51481 tggaagcgat tgcggctgct aaaatcggtg attttgagtt agcggatagg aaaatcgctg
    51541 atgcagaaga atcacttata caagcgcatc atgcacaaac agacatgttg acacaagaag
    51601 cacaaggaaa tcatatgcaa gtgacattat tgactgttca cagtcaagat cacttaatga
    51661 catctattgc gtttactgat ttagcaaaag aaatcatcga actttatcgt cgcgtataca
    51721 gcgaataaat acaacttctt ttttgaacgg aagaagaaaa atttttgaac ggatttttta
    51781 ttttgttata aggagaactc tctttatata aaaaacttat aggaggaagt aaaaaatgaa
    51841 cggactatca agttggatgg agaaatacat cctgccggtt gccgcaaaaa tcggagcgca
    51901 aaaacattta gttgctttgc gtgatgcttt tatcggcata ctgcccgcaa caatggcggg
    51961 atcagtcgct gtaatgatca atgcaattat tcgtgattta ccgcaacagt ttaacagtac
    52021 ctatgctgac agtttagcag cagacaaagg aatctttgcc gtattgaatg tcatcatcgg
    52081 aatcaatggt tttgtctgga acggtacact agcaatcgcc ggattgatct ttgccttttc
    52141 ttgggggtat aatcttgccc gagcttatgg agtaaatgat ctagctgggg ggatcgttgg
    52201 tttagctacc ttgattcaag gtatttcctt tggtccagca ttatcagcag cattagatgt
    52261 gaatgttcct tcaaatgtcg cagatgcaat caatagttct gagattggtg caacgattgc
    52321 agatggcaca ctgtcagttg gattatgggg ttggctaaaa ctagatcatt tgaatggaaa
    52381 cgcttatttt acagttatga tcatgggagc tatttcagtc attatttttt gtaaattgat
    52441 gcttgccaac attacgatca aaatgcctga ttcagtacca ccagcagtat ctaaagcatt
    52501 tgcagccatt ttaccagcga cgatcgcttt atacgtcatt gctattatca attatatcgt
    52561 aggtcgtgta gctgatgggc aactgatcat tgatttggtg caaaaatata ttgcagaacc
    52621 attcctagga ttatctcaag ggttgggagc agtattgatc gtcacagtat ttgttcaagt
    52681 attctggttc tttggtattc atggatcaaa tgtattggca ccagtgctag agggaatctg
    52741 gggacaagca caactaatca atattgatat tttccaaaaa ggttataacg gaaaaactgg
    52801 tacaccagcc gtgttagatg caatcgaaga tggaaaagct tatatgtggg tccgcggatc
    52861 gttcgatgct tttgcatggt ttggcggatc aggcggaacg atcgtcttac tgatagctat
    52921 cttgattttc tctaaacgtg cagattatct aacagtagct aaattatcca ttgggcctgg
    52981 tatcttcaat atcaacgaac cagtgatgtt tggtctgcca atcgtattga atgcaatcat
    53041 gtttatccca ttcttgattg ctccagtagt agcaacagca attggatgga cagctactta
    53101 tcttggccta gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc cgttcttatt
    53161 gtcattctta gcaacaggag cggattggcg ggcaccgatc gtaacattag tatgtatggt
    53221 cgtgactttc ttgatttggg caccatttgt catctctgca aacaaaatgg accctgccgc
    53281 aggcgaacaa caataagatg aacgtgagga gcgggacgct ttaagtttcg ttcctcataa
    53341 tgaattttcg aggaggaact gaaatgatta caattgccag tgcaatgcaa caagatgaaa
    53401 tcaaagaatt gttagaagga tacaatgaag aaggaatgac ttttacattt aaagaaaaac
    53461 aaggaatcaa gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag
    53521 caaaggaact aatcaaagca cagccttggg gaacagtcct ttattttcaa tcaacagctg
    53581 tttagaaaat ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt ttatttttat
    53641 gtagtattgg ttctttgatc gtgttgtata gtttgaatcc acattcgcaa aggtatgatt
    53701 tgtatagtat ggtcacaggt ctttttctaa caggattagg aatctatttc ttcatgaagg
    53761 ggaaaaaaag agaagaagca aaggagaaaa ataactaatg gaaatcacga aaaaattaaa
    53821 tattccagca tcgttttttt acgataaagt tatggactct gtccttttcg atgtacgtaa
    53881 agcgaccggc aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt
    53941 ctcgcaaaat aaccgggctc gcataaaaat cgaagaagtc attgaaaacc agtcttataa
    54001 gtttcgtaca tcgacaacga agaatgaatt cgtcgttcat tatcaaatca aaccattgga
    54061 cgattcgacc tgtgaggttc attacacaga aaaaatggag tcttatggca tgcttcaaaa
    54121 gttgaatgat atgcttttag gaacaatctt gatgtatttt aaacggagac gtttcaaaaa
    54181 aatgctagat atgatcgaag caacgtatta aatcaatgaa acatggtact atttagttat
    54241 tgaaaaatga agtaagctag aaggaatttt tcataggaag atagatcttt cctgcgaatc
    54301 ctcactagct tttttgtagg aaggggcagc aaaatgacaa acaaagtaag agagctaggt
    54361 gtatcttcga ttgaggaaat gtttgattta gccacttatg catttaatgg ggcagataca
    54421 cctgaaaggc gagaacgttt tcgtacactt gcggagaatt cttggaatta cggatttttt
    54481 gatgaggagg atcggctaac aagtcaagtc atggcgaccc cttttccagt gaatttttac
    54541 ggacaagagt acctaatggc cggcattggc tatgttgctt cttatccaga agcaagggga
    54601 caaggcggga tcaatcagat catggaaaag atccttgaag attgccgtga tcgtaaagta
    54661 agcttatctt atctggcgcc attctcctat cccttctatc ggcgctatgg ctatgaacaa
    54721 tcatttgata aaatcagtta tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa
    54781 tctggaaaaa taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag aagaatttat
    54841 gaacagatgc cggaaaacca acgagctggt ctgaagcgcg aagactggtg gtatacgtat
    54901 aaattcaagc aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa tgaggaagca
    54961 acgggatata tcgtttatca gcttgcaacg tcttccttta ttattgcgga gtggggattt
    55021 ttggatcatg atgctttttg cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt
    55081 gaaacatttg aatattcatg cggctacggc ggtaatgatc aaaattatct attagaaaat
    55141 ccatttgctt ctgtaatgat cacaccttat atgatggcga ggatcgtaga tatttctttg
    55201 tttttggaaa attatccatt taaaaagaga caagctgcat ttgccctaca aatagaagaa
    55261 gatcattatg cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat
    55321 catctctcaa aagtagaaca aacaaaactc ccaatcgttc gaggaagtat ccaacatatc
    55381 acgcagctat tattagggta tcgtaaagtg gaagagttgg tattccaaga aagaatacag
    55441 gtgacaaaag aatatgaaga aacgttaagc tcaatcttgc cacagcaaca gccaattctt
    55501 aatgactatt tttgataaaa aacgggtgat gacaatattt ttgtcaccac ccttttgttt
    55561 ttcatttggc atttgaagga aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt
    55621 ttttgttttc ttttgtttta gggttttctt ttttgtggtc ttggattgct tctttcgtaa
    55681 tcttttcccg atttttttta gatgaatcta agcgttctgc gtatactttt tcttttggct
    55741 tatcattcat tggaaaggac ctccctacat tatttcattc tacctctagt ttatcatctg
    55801 ttaaggacta gtggaaacgt tttgcctaac ttataagctg atttttttag cagataagct
    55861 tgcaagtaaa aaacaaacaa gctacaattg atttattgaa attgttcctg tagctcagta
    55921 ggatagagca atcgcctcct aagcgatagg tcgctggttc aattccagtc aggaacgtat
    55981 aaataacccc tgtatccttt gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg
    56041 ccaaactagt tatcattttt atataaacta tcgaacacag tattgatttt ttctttatcc
    56101 tgatcttcaa gctctcgaac aatatgaa
//
LOCUS       DAZKMK010000006        53934 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000006 DAZKMK010000000
VERSION     DAZKMK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.006"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(492..995)
                     /locus_tag="ACG8V1_000406"
     CDS             complement(492..995)
                     /locus_tag="ACG8V1_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0328528.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1354..2379
                     /locus_tag="ACG8V1_000407"
     CDS             1354..2379
                     /locus_tag="ACG8V1_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0328529.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2702..3580
                     /locus_tag="ACG8V1_000408"
     CDS             2702..3580
                     /locus_tag="ACG8V1_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0328530.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4056..4172
                     /locus_tag="ACG8V1_000409"
     CDS             4056..4172
                     /locus_tag="ACG8V1_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0328531.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5100..5438
                     /locus_tag="ACG8V1_000410"
     CDS             5100..5438
                     /locus_tag="ACG8V1_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328532.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5532..5762
                     /locus_tag="ACG8V1_000411"
     CDS             5532..5762
                     /locus_tag="ACG8V1_000411"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0328533.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6162..6533
                     /locus_tag="ACG8V1_000412"
     CDS             6162..6533
                     /locus_tag="ACG8V1_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0328534.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6807..7499)
                     /locus_tag="ACG8V1_000413"
     CDS             complement(6807..7499)
                     /locus_tag="ACG8V1_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0328535.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7502..8287)
                     /locus_tag="ACG8V1_000414"
     CDS             complement(7502..8287)
                     /locus_tag="ACG8V1_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0328536.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8524..9924
                     /gene="lacG"
                     /locus_tag="ACG8V1_000415"
     CDS             8524..9924
                     /gene="lacG"
                     /locus_tag="ACG8V1_000415"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0328537.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9949..10266
                     /locus_tag="ACG8V1_000416"
     CDS             9949..10266
                     /locus_tag="ACG8V1_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0328538.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10279..11952
                     /locus_tag="ACG8V1_000417"
     CDS             10279..11952
                     /locus_tag="ACG8V1_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0328539.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11966..13597
                     /locus_tag="ACG8V1_000418"
     CDS             11966..13597
                     /locus_tag="ACG8V1_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0328540.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14088..15653)
                     /gene="guaA"
                     /locus_tag="ACG8V1_000419"
     CDS             complement(14088..15653)
                     /gene="guaA"
                     /locus_tag="ACG8V1_000419"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0328541.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15712..16635)
                     /gene="coaA"
                     /locus_tag="ACG8V1_000420"
     CDS             complement(15712..16635)
                     /gene="coaA"
                     /locus_tag="ACG8V1_000420"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0328542.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16831..17436
                     /locus_tag="ACG8V1_000421"
     CDS             16831..17436
                     /locus_tag="ACG8V1_000421"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0328543.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17744..18760
                     /locus_tag="ACG8V1_000422"
     CDS             17744..18760
                     /locus_tag="ACG8V1_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0328544.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18901..20202
                     /locus_tag="ACG8V1_000423"
     CDS             18901..20202
                     /locus_tag="ACG8V1_000423"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0328545.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20248..20910
                     /gene="deoC"
                     /locus_tag="ACG8V1_000424"
     CDS             20248..20910
                     /gene="deoC"
                     /locus_tag="ACG8V1_000424"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0328546.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20931..21329
                     /locus_tag="ACG8V1_000425"
     CDS             20931..21329
                     /locus_tag="ACG8V1_000425"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0328547.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21395..22477
                     /locus_tag="ACG8V1_000426"
     CDS             21395..22477
                     /locus_tag="ACG8V1_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0328548.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22689..24251
                     /locus_tag="ACG8V1_000427"
     CDS             22689..24251
                     /locus_tag="ACG8V1_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328549.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24244..25377
                     /locus_tag="ACG8V1_000428"
     CDS             24244..25377
                     /locus_tag="ACG8V1_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328550.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25377..26333
                     /locus_tag="ACG8V1_000429"
     CDS             25377..26333
                     /locus_tag="ACG8V1_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328551.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26432..27598
                     /gene="deoB"
                     /locus_tag="ACG8V1_000430"
     CDS             26432..27598
                     /gene="deoB"
                     /locus_tag="ACG8V1_000430"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0328552.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27622..28440
                     /locus_tag="ACG8V1_000431"
     CDS             27622..28440
                     /locus_tag="ACG8V1_000431"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0328553.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28457..29164
                     /gene="deoD"
                     /locus_tag="ACG8V1_000432"
     CDS             28457..29164
                     /gene="deoD"
                     /locus_tag="ACG8V1_000432"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0328554.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29462..30148)
                     /gene="gpmA"
                     /locus_tag="ACG8V1_000433"
     CDS             complement(29462..30148)
                     /gene="gpmA"
                     /locus_tag="ACG8V1_000433"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0328555.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30320..30901)
                     /locus_tag="ACG8V1_000434"
     CDS             complement(30320..30901)
                     /locus_tag="ACG8V1_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0328556.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31034..31717)
                     /gene="rpiA"
                     /locus_tag="ACG8V1_000435"
     CDS             complement(31034..31717)
                     /gene="rpiA"
                     /locus_tag="ACG8V1_000435"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0328557.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32036..32449
                     /gene="rpsL"
                     /locus_tag="ACG8V1_000436"
     CDS             32036..32449
                     /gene="rpsL"
                     /locus_tag="ACG8V1_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0328558.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32533..33003
                     /gene="rpsG"
                     /locus_tag="ACG8V1_000437"
     CDS             32533..33003
                     /gene="rpsG"
                     /locus_tag="ACG8V1_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0328559.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33097..35184
                     /gene="fusA"
                     /locus_tag="ACG8V1_000438"
     CDS             33097..35184
                     /gene="fusA"
                     /locus_tag="ACG8V1_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0328560.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35356..36543
                     /gene="tuf"
                     /locus_tag="ACG8V1_000439"
     CDS             35356..36543
                     /gene="tuf"
                     /locus_tag="ACG8V1_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0328561.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36950..37258
                     /gene="rpsJ"
                     /locus_tag="ACG8V1_000440"
     CDS             36950..37258
                     /gene="rpsJ"
                     /locus_tag="ACG8V1_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0328562.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37289..37918
                     /gene="rplC"
                     /locus_tag="ACG8V1_000441"
     CDS             37289..37918
                     /gene="rplC"
                     /locus_tag="ACG8V1_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0328563.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37946..38569
                     /gene="rplD"
                     /locus_tag="ACG8V1_000442"
     CDS             37946..38569
                     /gene="rplD"
                     /locus_tag="ACG8V1_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0328564.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38569..38859
                     /gene="rplW"
                     /locus_tag="ACG8V1_000443"
     CDS             38569..38859
                     /gene="rplW"
                     /locus_tag="ACG8V1_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0328565.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38898..39731
                     /gene="rplB"
                     /locus_tag="ACG8V1_000444"
     CDS             38898..39731
                     /gene="rplB"
                     /locus_tag="ACG8V1_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0328566.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39774..40052
                     /gene="rpsS"
                     /locus_tag="ACG8V1_000445"
     CDS             39774..40052
                     /gene="rpsS"
                     /locus_tag="ACG8V1_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0328567.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40075..40422
                     /gene="rplV"
                     /locus_tag="ACG8V1_000446"
     CDS             40075..40422
                     /gene="rplV"
                     /locus_tag="ACG8V1_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0328568.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40436..41092
                     /gene="rpsC"
                     /locus_tag="ACG8V1_000447"
     CDS             40436..41092
                     /gene="rpsC"
                     /locus_tag="ACG8V1_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0328569.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41095..41529
                     /gene="rplP"
                     /locus_tag="ACG8V1_000448"
     CDS             41095..41529
                     /gene="rplP"
                     /locus_tag="ACG8V1_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0328570.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41519..41707
                     /gene="rpmC"
                     /locus_tag="ACG8V1_000449"
     CDS             41519..41707
                     /gene="rpmC"
                     /locus_tag="ACG8V1_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0328571.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41732..41998
                     /gene="rpsQ"
                     /locus_tag="ACG8V1_000450"
     CDS             41732..41998
                     /gene="rpsQ"
                     /locus_tag="ACG8V1_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0328572.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42056..42424
                     /gene="rplN"
                     /locus_tag="ACG8V1_000451"
     CDS             42056..42424
                     /gene="rplN"
                     /locus_tag="ACG8V1_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0328573.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42461..42769
                     /gene="rplX"
                     /locus_tag="ACG8V1_000452"
     CDS             42461..42769
                     /gene="rplX"
                     /locus_tag="ACG8V1_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0328574.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42797..43336
                     /gene="rplE"
                     /locus_tag="ACG8V1_000453"
     CDS             42797..43336
                     /gene="rplE"
                     /locus_tag="ACG8V1_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0328575.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43355..43540
                     /locus_tag="ACG8V1_000454"
     CDS             43355..43540
                     /locus_tag="ACG8V1_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0328576.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43576..43974
                     /gene="rpsH"
                     /locus_tag="ACG8V1_000455"
     CDS             43576..43974
                     /gene="rpsH"
                     /locus_tag="ACG8V1_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0328577.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            44006..44542
                     /gene="rplF"
                     /locus_tag="ACG8V1_000456"
     CDS             44006..44542
                     /gene="rplF"
                     /locus_tag="ACG8V1_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0328578.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44703..45059
                     /gene="rplR"
                     /locus_tag="ACG8V1_000457"
     CDS             44703..45059
                     /gene="rplR"
                     /locus_tag="ACG8V1_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0328579.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45080..45580
                     /gene="rpsE"
                     /locus_tag="ACG8V1_000458"
     CDS             45080..45580
                     /gene="rpsE"
                     /locus_tag="ACG8V1_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0328580.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45595..45774
                     /gene="rpmD"
                     /locus_tag="ACG8V1_000459"
     CDS             45595..45774
                     /gene="rpmD"
                     /locus_tag="ACG8V1_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0328581.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45818..46258
                     /gene="rplO"
                     /locus_tag="ACG8V1_000460"
     CDS             45818..46258
                     /gene="rplO"
                     /locus_tag="ACG8V1_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0328582.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46258..47553
                     /gene="secY"
                     /locus_tag="ACG8V1_000461"
     CDS             46258..47553
                     /gene="secY"
                     /locus_tag="ACG8V1_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0328583.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47615..48262
                     /locus_tag="ACG8V1_000462"
     CDS             47615..48262
                     /locus_tag="ACG8V1_000462"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0328584.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48453..48671
                     /gene="infA"
                     /locus_tag="ACG8V1_000463"
     CDS             48453..48671
                     /gene="infA"
                     /locus_tag="ACG8V1_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0328585.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48704..48820
                     /gene="rpmJ"
                     /locus_tag="ACG8V1_000464"
     CDS             48704..48820
                     /gene="rpmJ"
                     /locus_tag="ACG8V1_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0328586.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48838..49203
                     /gene="rpsM"
                     /locus_tag="ACG8V1_000465"
     CDS             48838..49203
                     /gene="rpsM"
                     /locus_tag="ACG8V1_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0328587.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49231..49620
                     /gene="rpsK"
                     /locus_tag="ACG8V1_000466"
     CDS             49231..49620
                     /gene="rpsK"
                     /locus_tag="ACG8V1_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0328588.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49700..50638
                     /locus_tag="ACG8V1_000467"
     CDS             49700..50638
                     /locus_tag="ACG8V1_000467"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0328589.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50666..51046
                     /gene="rplQ"
                     /locus_tag="ACG8V1_000468"
     CDS             50666..51046
                     /gene="rplQ"
                     /locus_tag="ACG8V1_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0328590.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51318..52157
                     /locus_tag="ACG8V1_000469"
     CDS             51318..52157
                     /locus_tag="ACG8V1_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0328591.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52133..53002
                     /locus_tag="ACG8V1_000470"
     CDS             52133..53002
                     /locus_tag="ACG8V1_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0328592.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52992..53789
                     /locus_tag="ACG8V1_000471"
     CDS             52992..53789
                     /locus_tag="ACG8V1_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0328593.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        17196 a         9664 c        11605 g        15469 t
ORIGIN      
        1 tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta ctacttctaa
       61 aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca gaatatactg
      121 gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat ataaagtaat
      181 tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta taaattaaga
      241 cggaattcct ttctagattg agatattgac taggaagaat gttaagattt taagccttta
      301 agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt ttcaaactgc
      361 aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt tttaaaccaa
      421 tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac ggtctttttt
      481 tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc tacgatcaga
      541 aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt tgtcgataga
      601 tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt attagttatc
      661 aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc tgccaagaag
      721 cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa attgacattc
      781 aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg taataccgtc
      841 gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta agaataagtg
      901 agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag aaagaataaa
      961 taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat ttaaaaagta
     1021 taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac caacgtagtt
     1081 atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa caaagctaag
     1141 atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc ggaatgttcg
     1201 ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat gaaaaatcaa
     1261 gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat cttggttcag
     1321 ctttttttcc aattactagg aaggagggat agattgttca agacttattt tcgtttcaat
     1381 aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt tattgtatta
     1441 tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt agggatcttt
     1501 acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat gtttctgatc
     1561 ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt tttatggctt
     1621 gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg attattccat
     1681 atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac gagcatgcag
     1741 ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt cgtttttgcc
     1801 ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac aattgaaaaa
     1861 gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc tgttttctac
     1921 agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca taatccttat
     1981 tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc gatgaagcat
     2041 cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc attcttgtca
     2101 tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata tggaatcgca
     2161 caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc gatggcgttg
     2221 atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata tcgtttttta
     2281 gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac ggttggtttg
     2341 caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata tatgtgccaa
     2401 aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa aagccagatt
     2461 ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa aaattttata
     2521 tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc tgagatggtc
     2581 aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta agttttatag
     2641 ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag tcatttaaac
     2701 aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca atgaaatcca
     2761 aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga ttgtacaagc
     2821 aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg atcctaataa
     2881 agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt ttgcagcatt
     2941 agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac cattgaagat
     3001 tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc atgaacattt
     3061 ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt acactagtgg
     3121 cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga aagggtacac
     3181 ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg atgcagattt
     3241 atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg aactttattt
     3301 tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac ccaatcttac
     3361 taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc gattattatc
     3421 agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag atgcccaaat
     3481 acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat tgcttgcagg
     3541 cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact tcgaaatagt
     3601 ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     3661 acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag
     3721 caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
     3781 ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa caatagatat
     3841 taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat gaaaatttat
     3901 ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt tgactttcat
     3961 tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt gttttttagc
     4021 atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat atctcaagtg
     4081 cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg aagatcatca
     4141 aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt aaaaaaatag
     4201 ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt tccgaaagaa
     4261 ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc ttttcaaatt
     4321 gataccattc cctaattcca gttatatagt taactacaga accacaaagc acgtacgttt
     4381 ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt ttgaccagta
     4441 gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt tagtcaagaa
     4501 tggaaacgta actacataat caaaggtttt gcgaaatatt tcatgaaata tttcatgtag
     4561 aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac gaaagctccc
     4621 aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag tgattcgtat
     4681 ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa tatgcaaaaa
     4741 aataaaaaag cagttactat tgctagaata acatcaagga ataataagtt ttggttttca
     4801 gataatagtc gataagttgt gtattggaat tttagataca gtaataaagt atttttcatg
     4861 aaattttgtg agaatcacta ttacaagtat ctttaataaa attaatggtt aaggaggaac
     4921 ttaaagtaga atgtaaatga atagttgtag tcagtcgatt gtgatactag actcaaagtt
     4981 gcaggaataa gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga agggcttttt
     5041 tttatacttt gttgattgta gtgtttattt tccctctgtt tagccaagta acattttcta
     5101 tgactcaacc agtttcaagt gaggaacatg ataacctttt tcgtgctaca gaagagttta
     5161 agaaatatca ggaaaatatt gttccggaga ctccattagc tagtgagcca attactgata
     5221 ttgatggaaa ccaaatagga tggtatatac agtacgaagt tatggtggta aataatgact
     5281 caatagtaaa aaatacagta ggcttagagc taacaccgat tgaaaaggtg agttcattgt
     5341 taacgtatat ttatactgat gaaagtgacg agactgagat cattttaacg gattactcaa
     5401 aaattgctac tgataaaaag tatatattgt ggatgtagac actagtgaaa tactttatga
     5461 tatagaaatt gatgaaaatt tactagatgt agcacaagaa atcgatcatc aaaagcagga
     5521 agctattcga tatgctggta gaaaagggaa aagaaaatat ttattacgtg aatgtagcaa
     5581 aagttagaga agatgaaaat gagtggaaag aattcaagag tcgctactca attaactcga
     5641 ctccgacatt tacagtttac agagaaggat cgatagaaaa aactgttttt tggactaaag
     5701 aaagtggaat gtctttagct gaagtagaag agtttttgga ttatgttagt atgcaacagt
     5761 aagggagttc ttattatgaa aaattttgac acatattatg ttattgtttt ttcgatatct
     5821 ttgctgttaa tattactaac tttaatgcag tataaagagt atccagagta ttttatttta
     5881 acttttgtat atttagtaag tagctacaca cttctatata aattcagaag ttcttctaga
     5941 gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg atttatatag
     6001 gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta atagatttaa
     6061 taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac cctgtagaag
     6121 aattttttca agattacttt gcggagtatg ccgataggat aatgagagat tacgtaacag
     6181 ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct gataaggcga
     6241 aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat atgagtggtg
     6301 aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt atcaaaactt
     6361 ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa ttaattaata
     6421 tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg ttgtttacag
     6481 ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt taaccattac
     6541 gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa gattggtttg
     6601 agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga acgcgtcata
     6661 tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg atatagcagt
     6721 gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca tttcagttgc
     6781 ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga caggtgctct
     6841 gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc ctgttcaaat
     6901 actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc cgcacgtttg
     6961 gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg agctaagctc
     7021 agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa agagccgtcg
     7081 tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc aaaataaaaa
     7141 gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg gtcagtcagc
     7201 tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt ttgcaagcaa
     7261 agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga ggcggtcaaa
     7321 tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat attttttaaa
     7381 atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag ccaagcagct
     7441 tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat ccctttcata
     7501 gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg acatattgtg
     7561 catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga tcgaaatcat
     7621 gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca acgagtagat
     7681 atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg gtactgtaag
     7741 tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta aactttagtc
     7801 ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc cggaaatcac
     7861 cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa actggataat
     7921 tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt tcgattgttg
     7981 tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct gtggcagcaa
     8041 tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt tcttgatcta
     8101 aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc gatttttcta
     8161 attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg atgatttcat
     8221 taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat ctttcttttt
     8281 tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga caacagtcaa
     8341 tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca atagatttta
     8401 ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa tgttttggat
     8461 gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg aggagagatg
     8521 tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc ctatcaggtt
     8581 gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt tttagaggaa
     8641 caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc aaaggacatt
     8701 gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg gagccgcatt
     8761 tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca tcgtgtgttc
     8821 gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt tgatacccct
     8881 aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc atttgtttcc
     8941 tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac ctttaatgaa
     9001 atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg aatcaaatat
     9061 gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc ccgtgtagtg
     9121 aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc attagagacg
     9181 aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga cgatgcattg
     9241 tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga aaccttgact
     9301 gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga agaagatttc
     9361 gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca ctaccaatgc
     9421 cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac gggtgaaaaa
     9481 gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga aggaattcct
     9541 cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt gctgcgaatt
     9601 aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat ggggtataaa
     9661 gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt gagagtctat
     9721 ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta ttttttgtgg
     9781 tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg cttgttttat
     9841 gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta caagttggtc
     9901 agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat gaataaagag
     9961 gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg cagtacatta
    10021 ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc ggcgttgaaa
    10081 gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt agcggaagaa
    10141 gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga tcatttaatg
    10201 accactttat tgctgagaga tctgattcaa gactttattg tactgtacag acagaatcaa
    10261 ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg gaaaccattt
    10321 tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt tttaacagcg
    10381 atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc tgagattttt
    10441 ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa ttattcgatg
    10501 ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga ttcgatgaat
    10561 cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc ctctgtggta
    10621 agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga atatatgggg
    10681 acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt atataagttt
    10741 tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac gatttcgcaa
    10801 accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt aattgattta
    10861 ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt gctgcagccg
    10921 ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc gatggcgctg
    10981 ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc ggcaattatc
    11041 tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc ttctaaagta
    11101 ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc aacattggtt
    11161 gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt tgggaaagct
    11221 tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc acccatgatt
    11281 ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt ttgcctgttc
    11341 aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc ttgggcaact
    11401 ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt tgttctagca
    11461 gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc atacgatgct
    11521 gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga aggtgcttct
    11581 gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga tgcaaataaa
    11641 gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgggatgtt agctaatgct
    11701 ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg tgcttatggt
    11761 tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca agtcaattct
    11821 tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt tgcgacaaaa
    11881 ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt tattctgaac
    11941 caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta aagctgtttt
    12001 atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag catctgccag
    12061 cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat ctggtgcaaa
    12121 atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata atggtgtcaa
    12181 ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa gttatggagc
    12241 agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa atctgggact
    12301 taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga aacagaatct
    12361 tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt acgactatac
    12421 aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg ttcagatcgg
    12481 caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag aaggcggggg
    12541 agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc gagacactca
    12601 acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag gagcatttac
    12661 ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg gagtgtcgta
    12721 ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg cgatcagcca
    12781 acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa cagcgaatgc
    12841 cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat atgaaccaac
    12901 accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg cagtaccgaa
    12961 taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa atgtgagttg
    13021 ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa acgaatggga
    13081 caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg cgtttaaaca
    13141 agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg gaccaacgac
    13201 cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta cgatcaaaac
    13261 ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc aagcatacga
    13321 ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat tgagtgccta
    13381 tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt atggtggagc
    13441 ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa tcaaagacat
    13501 tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag gaaatgcttg
    13561 gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt tcgattcaag
    13621 atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa cctgcacctc
    13681 gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac gagtaaagca
    13741 ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc gtaagaagca
    13801 atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa agggtaacct
    13861 tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag ttctcggaaa
    13921 acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta acttatatta
    13981 tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt ttagctattg
    14041 aagaatgttg atttataggg attccccctc catattatgt tggctcttta ttcccactcc
    14101 actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg ggctacttcg
    14161 tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc aaagtcagcg
    14221 gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta agtacgtccg
    14281 tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg ccagatatca
    14341 cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc gcgtacgatt
    14401 tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg accagggaat
    14461 ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc acggacttcg
    14521 tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc tggcagtcca
    14581 cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc gataacgtca
    14641 gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag ttttgttgct
    14701 tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg ttctgggtca
    14761 gatacacctt tcaacttgtc taagaagcga tctcttgcat tgactttgat gatgttcaag
    14821 ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg tagcagaccg
    14881 tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag aaccccaacg
    14941 acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc aacttgttca
    15001 cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc ttcacagtga
    15061 caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg acgtacttct
    15121 gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc aatcggacaa
    15181 tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa gtcaccatgg
    15241 ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg atcgcttgtt
    15301 acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga accgccaagt
    15361 ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc taaattgaag
    15421 atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc gccagaaaga
    15481 atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg gctcaataat
    15541 tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata ctgactaccg
    15601 tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt caaatttatt
    15661 acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat gctaatattt
    15721 tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta tatcggctct
    15781 tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt tccatacatc
    15841 ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat aataattctc
    15901 tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc atttttcgat
    15961 cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga cgtagatttg
    16021 ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat caggctgctg
    16081 gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa caggtacttt
    16141 tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct ccatatcata
    16201 actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac gatttggata
    16261 aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac gagacaaaat
    16321 cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg cgatcccgat
    16381 aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca agaacaaaga
    16441 ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga tatctcgaac
    16501 atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat cttgtgtcaa
    16561 aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac ggtagtagtt
    16621 cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat tttaacacaa
    16681 aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata atctttataa
    16741 tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg atagactgtt
    16801 gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc tgaacgccca
    16861 gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac ctttcaattt
    16921 gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg tgtcctgatt
    16981 gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt gggatgcggg
    17041 tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga aatggtcgac
    17101 attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca aatcgaacat
    17161 gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta tgcagctatc
    17221 gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt gacaggcgca
    17281 tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa acaaggagcg
    17341 ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt cacaaaagat
    17401 aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata gtggagcgac
    17461 gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt tgttgagaag
    17521 tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca gagtcgttgt
    17581 tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa gtcggtagat
    17641 aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt cttgggggtt
    17701 ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc tgaccatcag
    17761 agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt tgaataacaa
    17821 aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga atgaacatca
    17881 ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga ccatcggaat
    17941 gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg tagagagcta
    18001 tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc aagaaaatga
    18061 gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc tggccacacc
    18121 aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa tgccaatcat
    18181 tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa aggaatatga
    18241 gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga tcggcatgct
    18301 gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc gtcatgcgct
    18361 gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt tgactgtcca
    18421 aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg ctattttttg
    18481 cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg gcaaaaaaat
    18541 cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat acatgattcc
    18601 gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg cacgtttcct
    18661 gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg aaacaaaatt
    18721 aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta tattgcttat
    18781 gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca gtttactaaa
    18841 gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga ggatcaggca
    18901 atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc gaaagaggaa
    18961 atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca agtcagtgca
    19021 ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa cttaacattg
    19081 gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat aaaggtagat
    19141 aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc cctagtagct
    19201 agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac aggtggtaca
    19261 ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga agaatttgta
    19321 cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt ggcaccggcg
    19381 gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct tccgctgatt
    19441 gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt tttggatgtg
    19501 accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct ggcaaagacc
    19561 atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc agatatgtca
    19621 gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat cgaaacatta
    19681 caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag ccaaatggtt
    19741 gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca agaagctctg
    19801 gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg gggagaccca
    19861 gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga gcttcctgct
    19921 aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc tgcaatgatg
    19981 ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg attgaaactg
    20041 cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat ctatagtaac
    20101 gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat tggtgaatcg
    20161 gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa agcggacttt
    20221 accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga ccatactatt
    20281 ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc aaaaaaatac
    20341 catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga acaattacaa
    20401 ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa cacacccgag
    20461 acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt cgatatggtc
    20521 atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga tatcgaagca
    20581 gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac atcgttatta
    20641 gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc tgattttgtc
    20701 aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg tttgatgcgt
    20761 gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa cgaagaagaa
    20821 gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg agtagcaatc
    20881 gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc atggcagaac
    20941 tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat gttccttatt
    21001 cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat caaggcatca
    21061 acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct tttttcaaag
    21121 cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac acccccgatc
    21181 ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc cctgatatgc
    21241 cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt gcactgctgc
    21301 cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct gactgttgaa
    21361 aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat tatttggttt
    21421 aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca acggctctac
    21481 tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga tcacagacac
    21541 aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag aaaaatgggg
    21601 gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat cttcaaatga
    21661 atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa cgatttttgg
    21721 tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata caggtacgaa
    21781 ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg caactttcaa
    21841 agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg aaacaaacgt
    21901 agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg ctggattcaa
    21961 agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat tgaatcaata
    22021 tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta tgtatgcaca
    22081 aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat tccaagaagc
    22141 aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg accgcgacca
    22201 atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat tgacatctac
    22261 actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg acggtaaatt
    22321 ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc taacagaagg
    22381 acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa tcatttcagg
    22441 tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata gccttgttag
    22501 acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt atccaagctg
    22561 ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt caaaagaaga
    22621 acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg ttaggatgtg
    22681 aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc aaagaatttg
    22741 gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa attcatgcgt
    22801 tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc ggattattag
    22861 agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca agtccgacca
    22921 aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt gatgccttta
    22981 ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg cttgaccata
    23041 aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca gtcaacccag
    23101 atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt ttaaaaacac
    23161 tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg acccctcaag
    23221 aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag tcgatcattt
    23281 tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca gttatccgcc
    23341 gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa ttagccgata
    23401 tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag cctcaagaag
    23461 tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa gccgtgaaaa
    23521 acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc gatggaaacg
    23581 ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc ggacatatca
    23641 agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa catggtgtag
    23701 gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca ttatctgaaa
    23761 atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg ctgaattatt
    23821 cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga acaacgaatg
    23881 aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt atcgctcgtg
    23941 aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga ctggatgtag
    24001 gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac aaagcagtgt
    24061 tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt gctgttatcc
    24121 atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat gaattaggtc
    24181 tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag ggggctggcg
    24241 cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact ttcagttgtt
    24301 ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc catccaaggt
    24361 tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga aacgttaagg
    24421 caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc agctgggttt
    24481 ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt ctgggtggca
    24541 cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat cttaggtgcc
    24601 ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt tggaacaagt
    24661 gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac atttgcttta
    24721 catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat gatcagtcaa
    24781 aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg cgtaaactca
    24841 ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa gaaaacgact
    24901 ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta tgcaggaatg
    24961 agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc agggcttggc
    25021 ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac ttcgctatct
    25081 attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat cggtattctt
    25141 ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca gacaggagca
    25201 ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt tgtcgggatc
    25261 aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca agaagagatc
    25321 gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga actataatgt
    25381 cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca acaccgctaa
    25441 ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac gtagggctag
    25501 agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg atgagtccat
    25561 cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta ggtatcctat
    25621 tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt atcagcggga
    25681 cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca tggtatggta
    25741 aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca ggattagcag
    25801 atattccggt cattggacca attttcttta aaaatacatt cttacctgct tattgtgcta
    25861 ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc ttgcgcctcc
    25921 gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta tatgctatgc
    25981 gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct gtttttgctc
    26041 agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc atctccatgg
    26101 ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca ttgttctttg
    26161 gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct tcgattccgc
    26221 cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt gttttcttag
    26281 ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa taatctttta
    26341 gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt gttttaaagg
    26401 caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc tgatcgtcat
    26461 ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg ttggttctga
    26521 tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag agcagcttgg
    26581 tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc aaggctatgc
    26641 gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact gggaaatcat
    26701 gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg atgatctgat
    26761 caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt atagcggaac
    26821 agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa ttgtatatac
    26881 ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt tagaagaatt
    26941 atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata tgattggccg
    27001 aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa gcaatcgcca
    27061 tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag aagcaggaaa
    27121 ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa tcactgaatc
    27181 tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta tgaaaaacga
    27241 tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg gtcatcgtcg
    27301 ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc cagaattgct
    27361 tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa acgatccgac
    27421 gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca gtaaaaaaat
    27481 gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa cgattgctga
    27541 gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat taaaataatc
    27601 tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga aacgacccag
    27661 tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg ttcaggatta
    27721 ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga gatcccgttc
    27781 tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc tctggcaggc
    27841 aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc catgcaaaca
    27901 gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat tgtgactaat
    27961 gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat taccgatcat
    28021 atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat gggccctaga
    28081 tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa agcagcagca
    28141 gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg accaacttat
    28201 gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt agggatgtcg
    28261 actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg aatctcttgt
    28321 attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt tgtagaaaca
    28381 actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac attattgtaa
    28441 taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga tcgccgataa
    28501 gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt ttttggaaga
    28561 tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat ataaaggaga
    28621 acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct atgcccatga
    28681 attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg gtgcattgtc
    28741 aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca gttcttcgat
    28801 gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca atctattact
    28861 gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa atgttctttc
    28921 ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg aacttggcgt
    28981 tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat accatgtgca
    29041 gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa caacagctgc
    29101 agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag cgatagcaga
    29161 ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca agcaaaaata
    29221 gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt ttgaacaccg
    29281 tttaccaaga ggttgtgaat ctgccgttta actctgagtc acaaggaaca atttcaaaaa
    29341 acagcttttc atgtttgatg aatattgtga cttgtgaccg agaagttggc aggagaaaac
    29401 cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt tatagtgttt
    29461 tttataggta gtatttttta gcaactgtta agtcatcatt taattcataa acaagtggtt
    29521 gacctgttgg aatttcaaga tccatgatgt cttcgtcaga gataccttcg atatgtttag
    29581 ctagggcgcg taatgagtta ccatgtgcag ctactaaaac agttttgttg tctagtaatg
    29641 ctggagcaat ttcatcttgc cagaatggca aagcgcgttc taatgtcact ttcaagtttt
    29701 ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt gctgcagaac
    29761 cttcgtcagt ttcttccatt aatggaggaa gagtatcgta agaacgacgc cagatgtgca
    29821 cttgttcgtc gccgtatttt tcagctgttt cttttttgtt caaaccttgt aatttaccat
    29881 agtgacgttc gtttaagcgc catgatttga tttgtggtac ccaaagttga tctgaatatt
    29941 ctaagatcaa gttacatgtt ttgattgcac gagttaaaac agaagtgtaa gcaacatcaa
    30001 attcgattcc agcttctttg attttgcgtc cgccttcttt tgcttcttcg attccttctg
    30061 gtgctagatt tacatcagcc caaccagtaa attggttcaa tgcattccat tcactaagac
    30121 cgtgacgaga aaaaactaat tttggcatgt ttagctctcc ttttaagata ggtttatatt
    30181 tctgaaattt tcagttctgt tttattgtac aattttttgg aagagatttc catcgaaaaa
    30241 ttagataaga ttctatgaga aaacaagtag agaatataaa aaaacgaaca gtgcccacgc
    30301 aactgttcgt ttttaggtat tagatgcata attgctcgat ttctttttca ggtaaattga
    30361 tatatttata acatgttcca actgaaacac cgcattttga agaaataaat tggatcgttt
    30421 cttttttttc ataatagaga cgtcggattt ttttgatcgt ttttgcatcg atttttggtc
    30481 ttccgccgat ttttcctttt ttacgagctt catctaatcc aactaatgta cgttctttga
    30541 tcaatgcgca ctccatgttt gctaggtttt ccattaattg gaaatagatt tttcccattt
    30601 cgcgcgtatc gattccttca tcaagactaa tcaagtgcag cccgcgatct ttgaataatt
    30661 gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg caaatgacta
    30721 gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa atgaacagca gtagaatcat
    30781 tttgttccac ttcatatgtc tcatggaaaa tttcttgaca accaaagtca gataattgat
    30841 caacctgagt tgttaaatca acgtcattta tggttgtgcg tgcataacca ataattttca
    30901 tggtgattcc tttctagcag tgattttcta actggatata acagtaaaaa tgtagcataa
    30961 agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg tcttttttta ttggatgatc
    31021 aagcagtttt tctttattta cgacggattg cgtcaagaat ctcaacgcct ttttctgagc
    31081 cgacaactac tttagaaacc atgtttagga aaagcccatg ttctactacc ccaacaagtt
    31141 catctaaata ggtagctaag ctttctggct gttcgatgac ttctagatgc agatcaatga
    31201 tgtaatgacc accatctgtg gttaagactt ctccgttttc gtctgtacgc aagacgggtt
    31261 ggaatctttt ttcttcaaaa atatgaagta gctgttggga gccataaggg atcacttcaa
    31321 ctgggagtgg gaacttccca agtttatcga ctaatttaga agaatcaacg atccaaatcg
    31381 tttcttttga gtacgtagcg actattttct cgaaaagcaa agcagcgccg ccgcctttga
    31441 tcccttgaaa atcagcactg atttcatcag caccgtctat tgtcaaatcg actacgggga
    31501 cttcatcaat actctttaac ggtattccta atttttccgc ttgttctttt gtcgcatttg
    31561 aagtagtaac tcctgtgata cgtaaacctt cttcacgcat acggcgacca atctcttcta
    31621 ccatataata ggcggttgaa ccggtcccga gacctacgat catcccgtcc tttacgaatt
    31681 tcgcggcttc aatgccagct aattgtttta aattcatctt tgcgtccacc tttttttagt
    31741 ttgagcatcg tgctttcctc actattttag cgtgatttac ggtaaaaaga taccattctt
    31801 ttttattcca ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat gttttttcat
    31861 tcgtgatatt ctaacaaagg tgctttatct acaggtttct gtgaagaaac gtgctgactg
    31921 tttatcaaag catatttgat aaggatgcag acattgcatt tattttttat cacaaaaaaa
    31981 gaaccacctg gatgtgtgga actagacgca aatcgtggga aggaggaaac aaggaatgcc
    32041 tacaattaac caattagtac gtaaacctcg taaatcaaag gtggaaaaat ctaattcacc
    32101 tgctttgaac aaaggatata acagctttaa gaaaactcaa actaacgtaa actcacctca
    32161 aaaacgtggg gtagcaactc gtgtgggtac aatgacacct aaaaaaccga actcagcttt
    32221 acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt atatcccagg
    32281 gattggtcat aacttacaag aacacagtgt tgtattatta cgcggtggtc gtgtaaaaga
    32341 cttaccaggg gtacgttacc atatcgttcg cggtgcttta gatactgctg gtgttaacga
    32401 ccgtaaacaa agccgttcta aatacggaac taaaaaacct aaagcttaat tttaattatt
    32461 aaacaaatga aacttgcttc tactatatag aagtaattac tatgaacaat atttcggaag
    32521 gaggagttac ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt accagatcct
    32581 atttataact caaaattagt aactcgcttg atcaaccgtg taatggttga cggaaaacgc
    32641 gggattgcag ctaacattat ctataatgcc tttgacttga tcaaagaatc tacaggtaac
    32701 gatccattag aagtgtttga acaagcaatg aaaaacgttc aacctgttct tgaagttaaa
    32761 gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg aagttcgccc tgaacgccgc
    32821 gttactctag gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga acacacaatg
    32881 gaagaacgct tagcaaaaga aatcatggat gctgcaaaca acacaggtgc ttctgttaaa
    32941 aaacgtgaag acactcataa aatggctgat gccaaccgtg cgtttgctca ctatcgctgg
    33001 taagattctc tctgtttggt cggcttttat aagtcgacct cagatagtgt ccttattttt
    33061 agcaactaga tagtaaagag aggagaaaca aggagaatga ccagagaatt ttctttacaa
    33121 aacactcgta atatcggtat catggcccac atcgatgctg gtaaaacgac tgctactgaa
    33181 cgtatcttgt actatactgg taaaatccat aaaattggtg aaactcatga aggagcttca
    33241 caaatggact ggatggaaca agaacaagaa cgtggtatta cgatcacttc tgctgctact
    33301 acagcacaat ggaagggtca ccgaatcaac atcatcgaca caccaggaca cgtagacttc
    33361 acagttgaag ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt tcttgatgca
    33421 caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaacaac ttatggtgta
    33481 cctcgtatcg tatttgcaaa caaaatggac aagatcggtg ctgacttctt atattcagta
    33541 tcaactttac atgaccgttt gcaagcaaac gctcacccaa tccaattacc aattggtgcg
    33601 gaagacgact tcacaggaat tatcgacctt gtaacaatga aggctgaaat gtatacaaac
    33661 gatcttggaa cagagatcga agaaacagaa attcctgaag aataccgcga attagctgaa
    33721 gaatggcgcg aaaaattggt tgaagctgta gctgaaactg atgaagaact tactttgaaa
    33781 tacttagaag gtgaagaaat cactgaagct gagttaaaag aaggtatccg ccgcgcaaca
    33841 gttaacgtag aattctaccc agtactatgt ggttcagcat ttaagaacaa aggggttcaa
    33901 ttactattgg acgctgttct tgattacttg ccatcaccgt tagatatccc agctatcaaa
    33961 gggattgacc ctaaaactga tgaagaagta gaacgtcctg cagacgacag cgctcctttc
    34021 tcagctttag catttaaagt tatgactgac ccattcgtag gtcgtttaac tttcttccgt
    34081 gtgtactctg gtgttttaca atcaggttca tacgtacaaa acgcaacaaa aggcaaacgt
    34141 gaacgtgttg gtcgtatcct acagatgcat gccaactctc gttcagaaat ctctgaagtt
    34201 tacgctggag atatcgctgc tgctgttggt ttgaaagaca caactactgg ggacacacta
    34261 tgtgatgaaa agagtctagt tattcttgaa tccatggaat tcccagaacc agttatccaa
    34321 gtcgctattg aacctaaatc aaaagctgac caagataaaa tgggtgtggc tttacaaaaa
    34381 ctatctgaag aagatcctac tttccgtgct gaaacaaacg tggaaacagg cgaaacaatc
    34441 atcgctggta tgggtgaact tcacttagat atcatcgttg accgtatgcg tcgtgaattc
    34501 aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt ccgtgctggc
    34561 actcaagccg aaggtaagtt tgtacgtcag tctggtggta aaggtcaata cggtcacgta
    34621 tggattgaat ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa tgcgatcgtc
    34681 ggtggggttg ttcctcgtga atacattcca gctgttgaaa ctggtttgaa agatgctatg
    34741 gaaaatggtg tcttagctgg ctatccatta gtagatatca aagctaaact ttacgatggt
    34801 tcataccatg atgtcgattc aaatgaaact gccttccgtg ttgctgcttc tatggcatta
    34861 cgtgcagcag ctaagaaagc taatccagta atccttgaac caatcatggc tgtagaagtt
    34921 gttatccctg aagattattt aggtgatgtt atgggtcacg taactgctcg tcgtggacgt
    34981 gttgaaggta tggaagctcg cgctggcggc caacaagttg ttcgtgcgat ggttccactt
    35041 gctgaaatgt ttggttatgc tacaactctt cgttctgcta ctcaaggtcg tggtacattt
    35101 acaatgacat ttgaccacta cgaagatgta ccaaaatcag tacaagaaga aatcattaag
    35161 aaaaacggcg gaaaagctga ataatttctt gaaaccggat agtcctgcaa agagattatc
    35221 caaagagact atatttttaa tatagtttcg tgtaaaataa ttatcgatga tatgggcatg
    35281 tggtgtacct tgtgtacatc atatacctat cagacaaata aaaaatatat tgtattttta
    35341 ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca cgttaacatt
    35401 ggtactatcg gacacgttga ccatggtaaa actacattaa cagctgcaat cacaactgta
    35461 ctatctaaga aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg tgctccagaa
    35521 gaacgcgaac gcggaatcac aatctcaaca gctcacgttg agtatgaaac agatacacgc
    35581 cactacgctc acgttgactg cccaggacac gcggattacg ttaaaaacat gatcactggt
    35641 gctgctcaaa tggacggagc tatcttggta gtttctgctg ctgacggccc aatgcctcaa
    35701 actcgtgaac acatcctatt gtctcgtcaa gttggtgttc cttacatcgt tgtattcttg
    35761 aacaaagtag acatggttga tgacgaagaa ttactagaat tagttgaaat ggaagttcgt
    35821 gacctattaa cagaatacga attccctggt gacgatgttc ctgtagttgc tggatcagct
    35881 ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt aatggctgca
    35941 gttgacgaat acatcccaac tccagaacgt gacaacgaca aaccattcat gatgccagtt
    36001 gaagacgtgt tctcaattac tggacgtggt actgttgcta caggtcgtgt tgaacgtgga
    36061 caagttcgcg ttggtgacga agttgaagtt gttggtattg ctgaagaaac ttcaaaaaca
    36121 acagttactg gtgttgaaat gttccgtaaa ttgttagact acgctgaagc tggagacaac
    36181 attggtgctt tactacgtgg tgttgcacgt gaagacatcc aacgtggaca agttttagct
    36241 aaaccaggta caatcacacc tcatacaaaa ttctctgcag aagtatacgt gttgacaaaa
    36301 gaagaaggtg gacgtcatac tccattcttc actaactacc gtccacaatt ctacttccgt
    36361 acaactgacg taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt tatgcctggc
    36421 gacaacgtaa caatggaagt tgaattaatc cacccaatcg ctatcgaaaa cggtactaaa
    36481 ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga aatcaaagct
    36541 taattcactt aagttttaaa gtaaagcaac atttattcgg acgtaatcgc tcggaacgaa
    36601 ggtcttagga tctttgttcc gagctttttt atttattgca ggatcaggaa aaaagacagg
    36661 agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa tgaaacttta
    36721 tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata tatattataa
    36781 tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga tttcgtcggc
    36841 taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg tgacggaaat
    36901 tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga tggcaaaaca
    36961 aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat cagcggataa
    37021 aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc cattaccaac
    37081 tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt cacgcgaaca
    37141 attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac caaaaactgt
    37201 tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca aattataaaa
    37261 ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga aaaaagtggg
    37321 aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg tagaagctac
    37381 tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag ctattcaagt
    37441 tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc atgttgcaaa
    37501 agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc taggagaata
    37561 tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta ttgatgtaac
    37621 aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac aaagccgcgg
    37681 accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc cagttgcacc
    37741 taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc gcgtaactat
    37801 ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga tcaaaggtaa
    37861 tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag ctaaataatt
    37921 gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac aagatggaag
    37981 ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta atgaaagtgt
    38041 tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac acgcggttaa
    38101 aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag gaacaggtcg
    38161 tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag tctttggacc
    38221 aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag caatgaaatc
    38281 tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct tgaacttcga
    38341 cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg actctaaagt
    38401 attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta acttaccaaa
    38461 cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa atacaaaagt
    38521 gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat gaacttacta
    38581 gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga tgacaagaaa
    38641 tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc ggtagaagct
    38701 gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa attcaaacgt
    38761 atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac attgactgaa
    38821 gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga taactcatta
    38881 aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat ggtcgtcgta
    38941 acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa tcattgttac
    39001 aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta cgtcatcaag
    39061 gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa gataacgttg
    39121 ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc gcgttagtac
    39181 actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa gtgggtatga
    39241 aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca ttagaaaaca
    39301 ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt ggacaattga
    39361 tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac gtattgatcc
    39421 gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca atcggttctg
    39481 taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct cgttggatgc
    39541 gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca cacggtggtg
    39601 gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt caaccagctc
    39661 ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt cgtcgtcgca
    39721 aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc accatgggtc
    39781 gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt gaagctcaag
    39841 caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca gttttcccaa
    39901 gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca gtttacatcc
    39961 aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact tatcgtggac
    40021 atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat ccaaatggca
    40081 gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc tcgaaaagcc
    40141 cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc aatcttgaaa
    40201 ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc agttgcgaat
    40261 gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc ttttgttaac
    40321 gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc tccaatcaac
    40381 aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat aatcagtggg
    40441 tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg atgctaaatg
    40501 gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc gtaaatttat
    40561 ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg caacaggtcg
    40621 cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag gcggttctga
    40681 agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc atatcaatat
    40741 cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa tcgcacgtca
    40801 attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac gctcaatgcg
    40861 tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag cagatatcgc
    40921 tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg cggacattga
    40981 ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag tgtggatcta
    41041 tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat aatcatgtta
    41101 gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg ggaagctaaa
    41161 ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc acattggatc
    41221 acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa acgtggtggg
    41281 aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat tggggtacgt
    41341 atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg tggtaaaatc
    41401 atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg tctagcttct
    41461 cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg tgaatcgaat
    41521 gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag aaaaacaatt
    41581 aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag aaaacactgc
    41641 acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc gtgaacaagc
    41701 taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc aacgcaaagt
    41761 ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg ctgtcgaaac
    41821 aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat acaaagtgca
    41881 tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa ctcgtccatt
    41941 atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta ttatctaata
    42001 acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac acaacgtgat
    42061 ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa ttttgacgat
    42121 caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga tcgttgctac
    42181 ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta aagccgttat
    42241 cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat ttgatgaaaa
    42301 tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct ttgggccagt
    42361 tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac cagaagtttt
    42421 ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta aaaaaggcga
    42481 taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac tagcagcgtt
    42541 cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac accaaaaacc
    42601 ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc atgtttctaa
    42661 cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag aagtagacgg
    42721 caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa agtttaagga
    42781 aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag taactccatc
    42841 attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg aaaaaatcgt
    42901 catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata aagcagttga
    42961 agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa aatcaatcgc
    43021 tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc gcggagaaag
    43081 aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac gtgacttcca
    43141 tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta ttaaagaaca
    43201 attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta tggacatcgt
    43261 tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac aattaggcat
    43321 gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa tgattgctaa
    43381 aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac gttgcggacg
    43441 tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg aacttgccta
    43501 taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt cgcataagaa
    43561 ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac tcgtatccgt
    43621 aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat taaacgtgac
    43681 atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat cgaagatgac
    43741 aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg tgttatcaca
    43801 aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga cgaagtacca
    43861 aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt tgtcactgat
    43921 aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg gtaatcaagt
    43981 agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg ttgttatccc
    44041 tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac caaaaggtga
    44101 attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg aagtaacttt
    44161 cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc gtgcgaactt
    44221 caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac taatcggggt
    44281 tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt actcacatcc
    44341 agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca cacaagtaat
    44401 tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc gtggaactcg
    44461 tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg tacgccgtaa
    44521 agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag cagcaggaaa
    44581 aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc cgcagcttct
    44641 atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa gaggtgacaa
    44701 ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt cgtgtacgta
    44761 acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct aacaaaaaca
    44821 tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc tctaccttgg
    44881 ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt aaactagttg
    44941 ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt ggataccttt
    45001 accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta gaattttaga
    45061 agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat tagaagaccg
    45121 cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc tacgttttgc
    45181 tgctttagtt gttgtcggtg acaaaaacgg acacgtagga tttggtacag gtaaagctca
    45241 agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact tagttgaagt
    45301 acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg gtggtagaat
    45361 cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag ttcgtgccgt
    45421 attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa atacaccaat
    45481 caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag aagaagttgc
    45541 agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac aataatggct
    45601 gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca acgcgataca
    45661 gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc taatgaagca
    45721 attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat ttaatttaga
    45781 aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg aattaaaacc
    45841 tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat ctggtaacgg
    45901 aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg gtgtgcgttt
    45961 aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag gattcacaaa
    46021 cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct ttgaagacgg
    46081 agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg aaaaagctgg
    46141 aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag cagcaaaatt
    46201 ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg tgatctaatg
    46261 tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat cctatttact
    46321 gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg cgtaaatgct
    46381 gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt ggtaagtggt
    46441 agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat tactgcttct
    46501 atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg gtcgaaacaa
    46561 ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt agtattggca
    46621 tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt aggtttcgtg
    46681 aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc cggtacgatg
    46741 tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg cgtatcaatg
    46801 atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat ttatgaagat
    46861 tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt ggccatttta
    46921 ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc tgaacgcaag
    46981 atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta tctgccgttg
    47041 aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat tgctacgcca
    47101 aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta caaagtgatg
    47161 atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat tctgatcgtt
    47221 gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc ggaaaacttg
    47281 caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga agagtatgtc
    47341 tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt agtggcattg
    47401 cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt aggcggtaca
    47461 agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga aggcttaatg
    47521 ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt tcccctcaac
    47581 acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa tggggctgcc
    47641 aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta ttccgcacat
    47701 ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg gcctggaagc
    47761 aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg gaatcgtgaa
    47821 agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct ttccacgtac
    47881 gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa aaattgatgc
    47941 tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg gccgatttat
    48001 ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag ttgaagacac
    48061 atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac ctgagacggt
    48121 taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt attataaaga
    48181 acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat ttgcagatgt
    48241 acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta tgcagttact
    48301 cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga gaatcctcta
    48361 gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt tatcgtgtga
    48421 aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg attgaagtcg
    48481 aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta gaaaacggac
    48541 accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt atcttacctg
    48601 gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt attacttatc
    48661 gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag taagaccatc
    48721 agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg ttatggtgat
    48781 ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc tataaatatg
    48841 gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc acttacttat
    48901 atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg tgtatctgaa
    48961 gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc agaagtagat
    49021 aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa acgtttgatg
    49081 gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg tggacaaaac
    49141 acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg caagaaaaaa
    49201 taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa tcgtaaacgc
    49261 cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt caataacact
    49321 atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc tggttcatta
    49381 ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc agaaactgct
    49441 acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa aggacctggt
    49501 tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt gactgcaatt
    49561 cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg tcgtgtttaa
    49621 tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac ttttcgtttt
    49681 gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa aaattgatga
    49741 agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg ggactacttt
    49801 agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca tcactaatat
    49861 tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag aagacgtcac
    49921 tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag aagaaaaaac
    49981 ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta tcgttgatag
    50041 tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag gagctacgtt
    50101 ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg aaaataaaaa
    50161 agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg ttcgtcgtgt
    50221 gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata aattgacgat
    50281 ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt ctgcgaaaat
    50341 catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa atgctgaaat
    50401 catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga tcgaagagtt
    50461 agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata ctgtacaaga
    50521 acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac gtaaatcatt
    50581 agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag aagattaaaa
    50641 ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca catctagcca
    50701 acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac gtattgtgac
    50761 tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca ctttaggtaa
    50821 acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg aagttgccag
    50881 cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac tgttcaacga
    50941 tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga agacagaacc
    51001 aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc acactaataa
    51061 catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt tttccgagtc
    51121 tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca tcataaagtg
    51181 tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata aaaaaacatt
    51241 ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag gaactttagg
    51301 atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat tataaatacc
    51361 agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat aaaggtgagt
    51421 ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa acgatcaatg
    51481 gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg gatgaagaaa
    51541 atatttggac gatccgacag atggttggaa tggtttttca aaatccagac aatcagtttg
    51601 ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg attcccagag
    51661 aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct gagtttgctt
    51721 cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt gccggcgtgg
    51781 ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta gatccagaag
    51841 gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa ttgaccgtta
    51901 tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta gtgatgagac
    51961 aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt cctgaactga
    52021 tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg aaagaacgtg
    52081 gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg ttatggacat
    52141 ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac aaagagcctt
    52201 atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag ggcataccgg
    52261 aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa caagcggtac
    52321 agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt taaagcccat
    52381 ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg aagaaacggt
    52441 agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag aagcactagt
    52501 cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg aacgttcgcc
    52561 atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc ttgccatgag
    52621 gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg gcagaaaaga
    52681 aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg tattagttac
    52741 gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg aaaagggacg
    52801 aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc taaaagaaaa
    52861 acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta aagggatgaa
    52921 ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc attatgctgg
    52981 aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga gattccttga
    53041 tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc ggaatcattt
    53101 ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta ctttctgtct
    53161 tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg atctggctaa
    53221 tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact tattggagtt
    53281 ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt ttctgtcgat
    53341 tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct ttggaattat
    53401 cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca gtccacgaaa
    53461 tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac gaaacagaaa
    53521 agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac ctagttcaga
    53581 aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc aaccgtgcgg
    53641 aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga agaacgaaat
    53701 atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt gtattattaa
    53761 ctgtgctact gatcttttta agaggataaa cgttcagtgg gccgctatct attcttttct
    53821 tgctgatctg tttgcaagaa aggatgcaag cagtgtatga caagaaatat tagggtctgt
    53881 ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga aaaa
//
LOCUS       DAZKMK010000007        50102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000007 DAZKMK010000000
VERSION     DAZKMK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.007"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..296)
                     /locus_tag="ACG8V1_000472"
     CDS             complement(<1..296)
                     /locus_tag="ACG8V1_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0328594.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYG"
     gene            complement(298..495)
                     /locus_tag="ACG8V1_000473"
     CDS             complement(298..495)
                     /locus_tag="ACG8V1_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0328595.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(889..2616)
                     /locus_tag="ACG8V1_000474"
     CDS             complement(889..2616)
                     /locus_tag="ACG8V1_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0328596.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2609..3802)
                     /locus_tag="ACG8V1_000475"
     CDS             complement(2609..3802)
                     /locus_tag="ACG8V1_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0328597.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3989..4633)
                     /locus_tag="ACG8V1_000476"
     CDS             complement(3989..4633)
                     /locus_tag="ACG8V1_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0328598.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4727..4861)
                     /locus_tag="ACG8V1_000477"
     CDS             complement(4727..4861)
                     /locus_tag="ACG8V1_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0328599.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4863..6995)
                     /gene="feoB"
                     /locus_tag="ACG8V1_000478"
     CDS             complement(4863..6995)
                     /gene="feoB"
                     /locus_tag="ACG8V1_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0328600.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6992..7465)
                     /locus_tag="ACG8V1_000479"
     CDS             complement(6992..7465)
                     /locus_tag="ACG8V1_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0328601.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7866..8090
                     /gene="nrdH"
                     /locus_tag="ACG8V1_000480"
     CDS             7866..8090
                     /gene="nrdH"
                     /locus_tag="ACG8V1_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0328602.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8249..10408
                     /gene="nrdE"
                     /locus_tag="ACG8V1_000481"
     CDS             8249..10408
                     /gene="nrdE"
                     /locus_tag="ACG8V1_000481"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0328603.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10458..11423
                     /gene="nrdF"
                     /locus_tag="ACG8V1_000482"
     CDS             10458..11423
                     /gene="nrdF"
                     /locus_tag="ACG8V1_000482"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0328604.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11512..12651)
                     /locus_tag="ACG8V1_000483"
     CDS             complement(11512..12651)
                     /locus_tag="ACG8V1_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0328605.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12960..13673)
                     /locus_tag="ACG8V1_000484"
     CDS             complement(12960..13673)
                     /locus_tag="ACG8V1_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0328606.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13927..14628)
                     /gene="nagB"
                     /locus_tag="ACG8V1_000485"
     CDS             complement(13927..14628)
                     /gene="nagB"
                     /locus_tag="ACG8V1_000485"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0328607.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14862..16394
                     /locus_tag="ACG8V1_000486"
     CDS             14862..16394
                     /locus_tag="ACG8V1_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0328608.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16585..17787)
                     /locus_tag="ACG8V1_000487"
     CDS             complement(16585..17787)
                     /locus_tag="ACG8V1_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0328609.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17869..18558)
                     /locus_tag="ACG8V1_000488"
     CDS             complement(17869..18558)
                     /locus_tag="ACG8V1_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0328610.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18664..19032)
                     /gene="rplL"
                     /locus_tag="ACG8V1_000489"
     CDS             complement(18664..19032)
                     /gene="rplL"
                     /locus_tag="ACG8V1_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0328611.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19085..19588)
                     /gene="rplJ"
                     /locus_tag="ACG8V1_000490"
     CDS             complement(19085..19588)
                     /gene="rplJ"
                     /locus_tag="ACG8V1_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0328612.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19792..19919)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19998..20687)
                     /gene="rplA"
                     /locus_tag="ACG8V1_000491"
     CDS             complement(19998..20687)
                     /gene="rplA"
                     /locus_tag="ACG8V1_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0328613.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20791..21213)
                     /gene="rplK"
                     /locus_tag="ACG8V1_000492"
     CDS             complement(20791..21213)
                     /gene="rplK"
                     /locus_tag="ACG8V1_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0328614.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21556..22224
                     /gene="sdaAB"
                     /locus_tag="ACG8V1_000493"
     CDS             21556..22224
                     /gene="sdaAB"
                     /locus_tag="ACG8V1_000493"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0328615.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22237..23109
                     /gene="sdaAA"
                     /locus_tag="ACG8V1_000494"
     CDS             22237..23109
                     /gene="sdaAA"
                     /locus_tag="ACG8V1_000494"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0328616.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23200..24300)
                     /locus_tag="ACG8V1_000495"
                     /pseudo
     CDS             complement(23200..24300)
                     /locus_tag="ACG8V1_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24392..25279)
                     /locus_tag="ACG8V1_000496"
     CDS             complement(24392..25279)
                     /locus_tag="ACG8V1_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0328617.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25356..25901)
                     /gene="nusG"
                     /locus_tag="ACG8V1_000497"
     CDS             complement(25356..25901)
                     /gene="nusG"
                     /locus_tag="ACG8V1_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0328618.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26002..26172)
                     /gene="secE"
                     /locus_tag="ACG8V1_000498"
     CDS             complement(26002..26172)
                     /gene="secE"
                     /locus_tag="ACG8V1_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0328619.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26196..26348)
                     /gene="rpmG"
                     /locus_tag="ACG8V1_000499"
     CDS             complement(26196..26348)
                     /gene="rpmG"
                     /locus_tag="ACG8V1_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0328620.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26510..27154
                     /gene="cbpA"
                     /locus_tag="ACG8V1_000500"
     CDS             26510..27154
                     /gene="cbpA"
                     /locus_tag="ACG8V1_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0328621.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27210..27713)
                     /locus_tag="ACG8V1_000501"
     CDS             complement(27210..27713)
                     /locus_tag="ACG8V1_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0328622.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27726..27772)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28310..29275
                     /locus_tag="ACG8V1_000502"
     CDS             28310..29275
                     /locus_tag="ACG8V1_000502"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0328623.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29374..30546)
                     /locus_tag="ACG8V1_000503"
     CDS             complement(29374..30546)
                     /locus_tag="ACG8V1_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328624.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30533..31678)
                     /locus_tag="ACG8V1_000504"
     CDS             complement(30533..31678)
                     /locus_tag="ACG8V1_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328625.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31692..32616)
                     /locus_tag="ACG8V1_000505"
                     /pseudo
     CDS             complement(31692..32616)
                     /locus_tag="ACG8V1_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32733..33812
                     /locus_tag="ACG8V1_000506"
     CDS             32733..33812
                     /locus_tag="ACG8V1_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0328626.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33809..34437
                     /locus_tag="ACG8V1_000507"
                     /pseudo
     CDS             33809..34437
                     /locus_tag="ACG8V1_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34547..34774)
                     /locus_tag="ACG8V1_000508"
     CDS             complement(34547..34774)
                     /locus_tag="ACG8V1_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0328627.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34877..37465)
                     /locus_tag="ACG8V1_000509"
     CDS             complement(34877..37465)
                     /locus_tag="ACG8V1_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0328628.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37485..38144)
                     /locus_tag="ACG8V1_000510"
     CDS             complement(37485..38144)
                     /locus_tag="ACG8V1_000510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0328629.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38193..38696)
                     /gene="trmL"
                     /locus_tag="ACG8V1_000511"
     CDS             complement(38193..38696)
                     /gene="trmL"
                     /locus_tag="ACG8V1_000511"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0328630.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38917..39783)
                     /locus_tag="ACG8V1_000512"
     CDS             complement(38917..39783)
                     /locus_tag="ACG8V1_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0328631.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39892..40533
                     /locus_tag="ACG8V1_000513"
     CDS             39892..40533
                     /locus_tag="ACG8V1_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0328632.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40595..41956)
                     /gene="mgtE"
                     /locus_tag="ACG8V1_000514"
     CDS             complement(40595..41956)
                     /gene="mgtE"
                     /locus_tag="ACG8V1_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0328633.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41974..42873)
                     /locus_tag="ACG8V1_000515"
     CDS             complement(41974..42873)
                     /locus_tag="ACG8V1_000515"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0328634.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42875..43672)
                     /locus_tag="ACG8V1_000516"
     CDS             complement(42875..43672)
                     /locus_tag="ACG8V1_000516"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0328635.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43650..44354)
                     /locus_tag="ACG8V1_000517"
     CDS             complement(43650..44354)
                     /locus_tag="ACG8V1_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0328636.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44485..45061
                     /locus_tag="ACG8V1_000518"
                     /pseudo
     CDS             44485..45061
                     /locus_tag="ACG8V1_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45179..45856
                     /locus_tag="ACG8V1_000519"
     CDS             45179..45856
                     /locus_tag="ACG8V1_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0328637.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45935..47743)
                     /gene="pepF"
                     /locus_tag="ACG8V1_000520"
     CDS             complement(45935..47743)
                     /gene="pepF"
                     /locus_tag="ACG8V1_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0328638.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47919..48917)
                     /locus_tag="ACG8V1_000521"
     CDS             complement(47919..48917)
                     /locus_tag="ACG8V1_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0328639.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49143..49796)
                     /locus_tag="ACG8V1_000522"
     CDS             complement(49143..49796)
                     /locus_tag="ACG8V1_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0328640.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15002 a        10511 c         8781 g        15808 t
ORIGIN      
        1 tctccataat ctgctcgata taaaccattt attccagcag ttcttccaat ttcactcaaa
       61 tttcttatta attcctcttg atattcagtg aatcttattt tattagtata agcagtaaaa
      121 tatagataat tcaccaatct atttattaaa ggaccgggat ctaattcata tttttgtttt
      181 tctaatttta aatatacctt ttgtaaaacc tcaagaatat cagaatattt aggatcttta
      241 cttatagata tatcattata taattcatga acaatttgct tagcattttt tttcatttta
      301 gcacttccct ggaattgctc cacctaaaaa accacctata gacattcctg caatacaagt
      361 agttgccttg gcccaatcga ccgtacattt atttttagtg caatacactc catttccata
      421 atattttcca ctatgagtgg tacccccata gataagttgt gtctctttaa tagacaaaat
      481 ttttaaatgt ttcataatct aatcacccct aaattaatgt ttttagacta ttattctaac
      541 attcattaaa tttcaaaaaa caattccttc ttgaaacgta gcaaaataat tcctgaataa
      601 gaatttaatc acttttaagt atataaacta tgtatattct ttaatatttt ttgagaatgc
      661 acttcgtaca taataaactt taatcacatt agtctttcat acctacgatc gatctctttg
      721 actcgcaccc ataaattcat aatagtttgt cggcgtcttt ggacgtttcg aagatgagct
      781 acgcattctc tttttttata gacaaaaaag atagagacga acatggagct tccaaatttg
      841 gatatttgat ccaattttgg aagctccatt tatttcgtgt ctgtcttttt attttttcca
      901 gctttcgact aattcttggt tctctttgat ccagtctttt gctgcttgct gtggatcttt
      961 tccattgttg atttccaaca taactttttc catatctttt tctgaccaat ggaaattgtc
     1021 taatactttg taagcttctg gctggtcttc tttcaatcct tttcgaacca tcgtatggat
     1081 ctgctcttcg cttcccattc cgttttcagg atcttctaag tatttcagat catatttggc
     1141 aaacatccaa tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt tgatcgcttg
     1201 acctagtgca acggtcattg ctcctgaaga agatgtctct actttccagt ctttcaagtt
     1261 gtcatatttt tggatcgtgt tttcagcagc cgtaactaca ccagctcctg gctcgattcc
     1321 tatgattttc ttacctgcct gatcggttaa ctcatcgatc gaatttacat ccatgtaggc
     1381 tggcactgct aaaccaactt ttgctcctgt caaattggca cctaggtctt cgacttgatc
     1441 tttatattgt gcatattgtg aaccatgagt tttaggtagc caagctgaaa ccatggcatc
     1501 tgactcgcca tttgaaacag atttccacat gattgaatta tctaatggcg tcatcgttac
     1561 atcatagccc atttgtttca atacttcacc gactacattt gttgaagcaa cttctgtatc
     1621 ccattcaaca taagataagt tgatacgttt tgtttctttg gcagatgaaa aactccctat
     1681 accaatcgca cctaagatca ctactactac agcaccaatg atcagtcctt gacgtttttt
     1741 acttttctgt gttgttgctc ctgcagcttt tttattgttt ggctggttca aatgctgtgt
     1801 aaaacggtca atgatgattg ctaagataac taatgcaacc ccgttgacaa atccattccc
     1861 gacttgtgct ctttgcaatg ctgataatac gccgcgtcct aagccgggtg caccgatcat
     1921 agaggcaata acgaccattg aaagtgacag cattgttgtt tgattgatcc ctgccatgat
     1981 cgtactttta gctaaaggca attctagttt gaatagtttt tgacgacttg tgctcccgaa
     2041 tgaatcagaa gcttctacta attctttagg aacttgtcga atccctaaat tcgtaaaacg
     2101 aacggttggt ggcagagcaa agatgactga ggcaaatacc cctggaacca tcccgatacc
     2161 gaaaaatgca actgctggaa tcaaataaac gaatcctggc attgtctgca taaagtcaag
     2221 gatcggtttg ataatacttt gggctttact gctttttgcc atcaagatcc ctaatggtac
     2281 cccaatgatg atcgagatca cactagataa aagaacaagt gtgacagtat tcatcaaatc
     2341 attccataga ccttggttat aaataaacca tagaccgatt aatgtgaaca acgtaagtcc
     2401 tggacgtttc ttagaaataa agaaagctgc taccgtcaat aacaagataa acaaaggagc
     2461 tggaatcact aacagtaaat ttgtaatccc cgacatcact gcctgtccaa tcgtttgtaa
     2521 aaatgaaaat aggccagaaa aagttgtcgt aaaccattct gtgatatttt ctacccagtt
     2581 tgccactggt aattgatagt tcaatagatt attcatgttc actcacctcg cttgtttctg
     2641 ctaatgcttc aatcacacta cctcggatga cgacaccttt taattttcca tcctctacaa
     2701 ctgcaagtgg agtcggtgag tcataaataa tattgaaaat atcacttacc accatgtctt
     2761 tcccaatcac cgtcacattt tcatctagga agtcgatcaa tggacgtttt tgcttcctag
     2821 cttctaacgc ttgatctgca gtgatgatcc ctttcaggtg tctctttctg tcaacagcca
     2881 tcagcatgct gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt ccatcagatt
     2941 cgatattcgt cgtcaatgca ggtaccatga tgttttgagc cgtaagcact ttggaacgat
     3001 cgacttcttc cacaaattca cgtacataat catttgctgg attggtcaat atttcttcac
     3061 ctgttccgat ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc ccaatccgta
     3121 aagcttcatt caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg gcttgcaggt
     3181 ccagtaattc gtcttgcatt tctcgccgga tcagcggatc aagagccgag aacgcttcgt
     3241 ccatcagcaa gatctcagga tcatttgcta aagctctggc taggccaacc cgttgttgca
     3301 tcccaccaga taactgacta ggatattggt ctttaaatga aagcaaacta gaattttcta
     3361 atgctttttc tgctttttct tgtcgttctt ctttaggtac accacggact tctaacccgt
     3421 attctgtatt ttccaagatc gttcgatgtg gaaacagacc gaagttttgg aaaaccatat
     3481 tgattttatg acgacggact tctcgcaatt cttctttgct taacttcgat acatcttctc
     3541 catcaatata gatactgccc gaagttggct caatcagacg attcaataga cggatcaatg
     3601 tggatttccc actcccggaa agccccatga tcacgaagat ttctccttct tttacgtcaa
     3661 aattcacgtc atacacacca accgttgcac cggttttctt taaaatctcc gttttcggct
     3721 ggtgatcatt catcatatct aaagcggctt gtgtcttttt accaaatatc tttgttaaat
     3781 gttttacttc tacttttacc aaacaaattc tcctttttta tgtaaattca tttttctttc
     3841 tgaaaaaagt gaccacacta tgttaacatc atgtagtcac tttgtcaaaa atgaaagaaa
     3901 tttgcttgtc caaactgaaa aaaacttggc taaatctttg taaataaaag gtttagccaa
     3961 gttaatattt ttttaatatt tcacacaatc attctgcaag ctgatgctcc gggtaaaaaa
     4021 agtttgtcat acgctgtagt gtatattcat ttccaataaa atgaacgatg tcccctgccg
     4081 taaatttagc ataggggcca ggtgaaacaa gcagttcttc attatgagaa attgcgataa
     4141 ctgtggcgcc agtcgcctgc cagatattca actcgctaat tgaacggtcc agatgcattg
     4201 ctttttctga taaagataat tcaaacggaa gtaaaggatt catcttatgt attcgcttcg
     4261 tttgattgac cagctgatcc aataattgag aaaaatgatt cagctcatct tgttgcttct
     4321 ccacacttgc catgatttct gcttttgtat cttggatgga ttgtacatct tgatattgca
     4381 ataggaaatc ttttgctttt tccttcgaag caacaaatgc cccacttcca tgccgtactt
     4441 ccatgattcc aagatctacc agcacattga tagccttgcg ggtagtctca ggagaagcat
     4501 tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc acccacttta tatcggtttt
     4561 caacgatacg ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg acgatttttt
     4621 tctttccaga cattttcttt cctctttctt tttttacttt ccctcatctt actatgctct
     4681 agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat cgcttgatct
     4741 gttccttttt tactgggcaa gctgtttgac agtcatcaca attcttaccg gattttaaac
     4801 gagtatagac aattgcgcct gcacttccaa agattaacac acttaataag aatgtcgcca
     4861 tcctattcct ctccttctaa tgacaatata gtataaaact ggttcttgga ttttggctga
     4921 cggaacaaaa cataaacgag tcctatcatc agtaaaatag caagatacgt ccctaacgct
     4981 acctgtccac cctccaataa gtgtcccaac tgataaatca caaaactaac taaataagct
     5041 aaaccgcatt gatagcctat ggcgatccaa gtccatttct tggcattcat ttcccgatgg
     5101 atagccccaa ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa caatgaataa
     5161 gctgctgcgg gagtatatgt gtgttgtagc acttgccaga tttctctgcc attttcggat
     5221 acttccgcat gaccaaacaa aataccaaat gtgccaataa tattttcttt agcgaccaat
     5281 cctgtaatcg ctgcgactgt tccttgccag tttccccaac caagcggctg aaaaacaact
     5341 gccaggattc gcccgaaaaa ggctaagata ctttggtctt cagaaacagg ttgagcataa
     5401 aaattataag aagagctgaa ccagatcacg atactagaaa caaagataat cgttcctgct
     5461 tttttaacaa aagacttgct gcggtcatag gtttgtctta ggaccgctga ccattgagga
     5521 aaatgatatg cgggcaattc catgataaaa ggagctgtat ctccagcaaa caaacgtgtt
     5581 tttttcaagc tgattccaga gaaaataatt gcagtcatac caagaaaata ggcagaagga
     5641 gctacccaag attgatgagg aaaaaaggcc cctgctacta aggcaataat cggcaacttt
     5701 gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg ctcgttttca
     5761 atcgtgcgac tggccatgac gcctggcact ccacatcctg tagcaatcag cataggaatg
     5821 aaggattttc ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa agcgatcctc
     5881 gccatatatc cgcagtcttc cagaaagctc aagcataaaa acaatataat caattgcggt
     5941 agaaatccta acactgctcc cacgcctgcg atgatgccgt tcaagatcag ctcctgcatc
     6001 catcccgcta cttgccattc agctaacaat ctgccaacat gttctggtaa ccatgtacca
     6061 aacaatgtgt cattgatcca atctgtcccc attgttccta ctgtttgaat agccagataa
     6121 tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta accaacgatt cgttgcaatc
     6181 tgatcgattt tgtcactcat cgataattgg aaagaatcat ttgacacaac acagagtgta
     6241 catagttggg taataaattc ataacgttca ttgaccagaa tcgtctcact atcgtcgtcc
     6301 aatagtttct ctgttaactg gatcagttgc tcgatctctt tttgctgaga aactgctatc
     6361 tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt tcattgcata ccagcgtttc
     6421 ttgccgcttt gttctcctag tacatcttca atttcatgta aagctgcttc taaacgtgga
     6481 tcatatgtac agggaacagg aatagaaggc cgcttttttg cagactcctg aatcttttcg
     6541 attagttttt cgattccttg ctttttcaat acgctgattg gtaaaactgg tacaccaaga
     6601 ccataggaaa gcttttctag attgattttt tttccttctt ttttgatgac atccatcata
     6661 ttcagaccaa tgatcatagg aatgtctaaa tccaataact gcgtagttaa ataaagattt
     6721 cgttctaaat tggtagcatc aataatgttg atgatcagtt caggctgttc ctgaaccaaa
     6781 taatctcttg caatcacttc ttctggtgta taaggagaca aagaatagat gccgggtagg
     6841 tcctgaatat gaatgttcgg attttttttc aacgatcctt cttttttttc tactgttacg
     6901 cccggccaat tccctacata ttgattcgaa ccagtcaaga gattgaacaa gctagttttc
     6961 ccgctgtttg gatttccgac taacgcgata ttcattcttt ttcctccctt tgtatcaaga
     7021 ttagttcggc ctctgacttt cgtaacgtca gttcataccc tcgcactgtc agttcgattg
     7081 gatctcccag aggggctagt tttcggataa ctatttttac atttcgcgtc aatcccatat
     7141 ccattaaccg tcgcttcact gcgccttgtc cgtggattgc tacgacgatc ccagtctctc
     7201 ctgcttttaa ttgatcaaga gaagtccagc tttcagagga agcagtcttt ttattgacaa
     7261 agatttgacc tagtatttcc cgacttactg ccactcgatg actttttacc agtaaaatgg
     7321 cattgttgcc tgataaattg acgacgacga ttttacttcc ggcaacgatc ccgatatttt
     7381 gcaaatggcg ttttgttttt tcttctgttt cgattcgttc gacttgataa acgaaattct
     7441 gctttgcctg cgctaaggtc tgcatattga ttcctctctt gtgaaataaa tcatgtatta
     7501 aaatcattca ttatatgttc taattttctt acgtgttacg caaataaatg agaatcattc
     7561 tcacatagaa tactctcggt aattgagaat gtcaattata cttgtgatat tatgagcgaa
     7621 tctttagtat tgatagataa aaatgagaaa aaaatatttc ttcatagttt tccacaattt
     7681 tttctctatt aaatttgcta ttttcccaca cgatacttgt tttcagctct tttttcaaaa
     7741 acacaaaact tagtattcca cagtttccac aacacaatat gtagtgtttg ttgtttgact
     7801 tttttactaa gagttttata ctatctatta tagataataa gaatgtgtta gaggaggcac
     7861 tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag atggtcaaac
     7921 ggtttttaac agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa cagcctgaag
     7981 cagtagactg gttgaaagca caaggattcc aaagtgttcc tgtcattact tctgatgcag
     8041 aaacagttat cggattccgt cctgatcagt tgcgtaaact tgccagctaa catgactaaa
     8101 agaatcaaaa cgtgatacat ggatttgaag ggcttgagac agcaccagtt tttgtcgcaa
     8161 acttccaaat ccttttttaa agcttaaaaa caatgcaatg atttgcaatc aagcatcatc
     8221 aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag atgtcagtta
     8281 tttcaagctg aataatgaaa tcaatcggcc agtcaatggt caaatccctt tgaataagga
     8341 taaagaagct ttagttgcct tttttgagga aaatgtcaaa ccaaatacga tgacttttct
     8401 atctattatg gaaaaaatca attacttgat cgatcaagaa tatatagaag cagaattcat
     8461 tcaattgtat tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt
     8521 ttctttcaaa tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa
     8581 cgacggttcc gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt
     8641 tgcgaacggg gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata
     8701 tcagccagct acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc
     8761 ttgtttcttg actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc
     8821 tttgcaattg tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc
     8881 cggcgctcct atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt
     8941 attcgaagat agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt
     9001 ttatttgaac gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc
     9061 agatgaaaag attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta
     9121 tgaattgaca cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga
     9181 atatggtgtc ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa
     9241 tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt
     9301 acaacaagaa tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc
     9361 aatcgatggc aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc
     9421 ttctgtgatt aacggcaaac aggaatatga agtattagga acggatatct catgtaactt
     9481 aggctcgaca aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac
     9541 gatgactcgt gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat
     9601 ccaaaacgga aataagctga atcatacgat tggattaggt gccatgggct tacacactta
     9661 tcttgcaaaa gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta
     9721 cttcatgctg ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa
     9781 acaagttttc cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata
     9841 cgtaactgga gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt
     9901 tatcccaact gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta
     9961 tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag
    10021 tgcaagtatt catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa
    10081 aatttattac ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta
    10141 tgatatggac atgcgtaaag taatcgatgt ttatgcaatg gcacaacaac acgtcgatca
    10201 aggcatgagt ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa
    10261 gacaacaacg aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa
    10321 aggaatcaaa tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag
    10381 caatcaatgt gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta
    10441 attaaaggag cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg
    10501 aagatatcat tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac
    10561 gtatcccact ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata
    10621 cagtaggaca tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca
    10681 tggatcagtt gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc
    10741 aattcatgga atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga
    10801 aaaaagaaat cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag
    10861 ctgagcgtat caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg
    10921 tctttttaga aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa
    10981 acaacaaatt ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac
    11041 atggaaccta catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac
    11101 aagaaacgtt aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac
    11161 gttatacaga agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac
    11221 gctataatgc caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg
    11281 ccaatgatgt caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact
    11341 tcttctcaca agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg
    11401 attatttgat tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa
    11461 aagagtttag cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact
    11521 tgtttcttcc agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa
    11581 taaatagatt ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca
    11641 aatcagcatg cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt
    11701 caaaggatgg atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa
    11761 ttgttggatc actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa
    11821 accgacaatg attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt
    11881 gattccgcaa atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa
    11941 aaagctcatt acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa
    12001 tgtttcgttt aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa
    12061 gtgagacaac gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg
    12121 aacggtaaat aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc
    12181 agataaactg actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa
    12241 ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt
    12301 gcttgtttca tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa
    12361 ctgaggcact acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa
    12421 taaaaaaccg aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa
    12481 catataatag ataaaaccag caacaagcaa tggtaaaaaa actgccgaaa gcattttcaa
    12541 tattggacta aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac
    12601 tagctccact gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct
    12661 ccaaggtcct ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc
    12721 atttgcttaa aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac
    12781 aaaaacacgg tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg
    12841 gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat
    12901 ttttatttgg ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt
    12961 tattgcgcaa tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg
    13021 atcacatcgg cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact
    13081 cggtagtcat caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct
    13141 tcaaaataat cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac
    13201 tttttagtcg taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg
    13261 ataaaactga tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca
    13321 attgcccata aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc
    13381 gttcccgcgc ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct
    13441 gaacgaatcg ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg
    13501 atctcaactt ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc
    13561 atacctgctg gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata
    13621 gcgatcccta aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca
    13681 ccctccagtc caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct
    13741 cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg
    13801 ttcaatcagc tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc
    13861 ctttgtcttc tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat
    13921 tgaaaattac aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt
    13981 ttgcaataca cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc
    14041 atcagctttg ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc
    14101 gattcccatt gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt
    14161 tgcattgatt gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc
    14221 aggttcattg aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg
    14281 atgtgcatcg atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc
    14341 atctggtaca aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg
    14401 acggtagctt tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt
    14461 catttcactg aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct
    14521 tcctgttgcc aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa
    14581 tgcttttttt ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc
    14641 tttatatttt ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat
    14701 agaccaaatt gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca
    14761 taagatggtt caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg
    14821 acgctttatg tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa
    14881 acaacattac ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa
    14941 atgctggcga acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta
    15001 aaatcatcac tggtgagatc ttaccagatg acggacagat ccattggcaa aaaaatattc
    15061 acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc
    15121 taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa
    15181 catatagcga gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat
    15241 tagaccaagg agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg
    15301 ggatcgatgt cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa
    15361 aggtgatttt agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa
    15421 ccaatcactt agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg
    15481 gtgcgtatat cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg
    15541 cagacatcga gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac
    15601 aaaaggaaca aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg
    15661 aaaagaccga agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa
    15721 gccgacaaaa acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag
    15781 ctgtctttga ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt
    15841 tagtcatcgg atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg
    15901 aaaatattgc cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga
    15961 tcggtgagat tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt
    16021 acttttcaca agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca
    16081 gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag
    16141 ttaatcaatt agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga
    16201 aacttgcaca gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc
    16261 atatcgatca agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag
    16321 taatcatcgt ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga
    16381 ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt
    16441 gtcacaactc cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca
    16501 aaaaacggca aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa
    16561 cagcttttca caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa
    16621 aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc
    16681 taaccagcca tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat
    16741 aaagtacata aacataaata cagtattatt tcggttgctt gctgcttcac ctagcccctg
    16801 tactcgtgtt tgattggcga cttgaccaaa ttgattcccg acgtctaata aaataatagc
    16861 taaaagcaaa atggccaaat ggcttccgcc aaccaataaa gcaataaagc ttattgtttg
    16921 cataacaagt cccatataca cgatttttct ttcagaataa gaatctgcca gtcgtcccac
    16981 gataggtgca gcaactgctc cagacaaacc aaaaatcgct aatatccctg cttcgaatgt
    17041 tccccaattg taggcgggac tgctgatata aaaaatcaac gtagaccaaa agatcgaaaa
    17101 tgttccaaac atgaaaaatc cactgactgc cgcttctctt aatagacgtt gagaagcaac
    17161 taaatgcggc aaacttttaa gtgaatcaag gtatgaaaca gctgtactac tttgctggac
    17221 agaacgcggc attttccagt aaagcagaag tgtaagaatt ccgactgcac ataaagcaaa
    17281 taaatagatc atccgccaag aaaaaagact ggcaacaata ccagataccg tccgagaaag
    17341 caaaatcccc acgagtaaac cgctaagcaa tgtacccatg accttccctc ttgcttttgg
    17401 tcccgctagt accgcaccgt aaggaatgat gatttgcggc acgatcgaaa gtaatcctat
    17461 taaaaaagaa gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg ccaacagaga
    17521 gacggcagat aacgctgcca ttcgaatgat caattttgga cgatccacca cgtcccctaa
    17581 aggaactaaa aacagtaacc ctaacgcata gcctaattgg gtcaacatcg tcaatatccc
    17641 gactgcactt gtgctaacag ataaactagc tgctactttt gttccaatcg gctggatgta
    17701 atacatattg gcaacaacaa cgccacaagt gattgataat agtaaaataa gcgaagttgt
    17761 taaaggtttt ttttcattac tcttcattga tttcccccat ttcctatctt agtttacaac
    17821 ttatggaaaa gattggaaat agaaaaatac aactattcgc cacacttatt atttgtgctt
    17881 ttgttttcct tgttttttct tgcgatcttt ataaatgaag acaactaacc ctactagcac
    17941 agtaacactt gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca gagcttctcc
    18001 accacacgca tacaacacag acgaagggat gactccaatc acgatcgaca atatgatttg
    18061 ctgtagaggc aatttcatcg catctgcagc aaaattgacc atagaagatg gaataaccgg
    18121 tatcatatag cctaacatta cacctatttt tggatgtttc atttgtcgga ttgcttgcac
    18181 ccaatgattc gtttcggttt tcttatctaa aaattttaag cgatgcataa ggaaaattgc
    18241 agtgatgtta ccaagagcat tacccaaaat gttgatgatc gcacctgtca gtggtccaaa
    18301 gctcaaacca gcaatgactc caactactga agtaggaata cctggaacag cacacatcac
    18361 cgctgtcaat acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa tatgtgcgag
    18421 tagctctgcc ttactcgctc tcggatttaa aaataattgg atgtctgtct gatattctaa
    18481 atacaaccga tagcccacta ccagtatcag aatgattccc aacgagatca atatcaatcg
    18541 gattattttc tttttcatat ttcctcctga aaaaaatcta tattcattaa ataatagttt
    18601 aacataaaaa caaaaaagca ccgaaatcca acataggatc tcagtgcttt taaatagtaa
    18661 aaattattta acagttacag atgcgccaac ttcttcaagt ttagctttca attcttcagc
    18721 ttcttcttta gatacgcctt ctttaactgg tgcaggagcg ccatcaacta cagctttagc
    18781 ttctttcaag cctaaaccag ttgcttcacg aactgctttg ataactttaa ctttttggtc
    18841 tccagctgca gttaattcta cagtgaattc tgtttgttct tcagcagcag cagcgccacc
    18901 agcagcagct acagctacag gagcagcagc agatacgcca aattcttctt caatagcttt
    18961 aactaggtcg ttaagttcta ggatagttgc ttcttttaac tcagcaacga tgttttcaat
    19021 gttcaatgcc attataattt cctccgttta attgttcttt tttatttaat gctttatgcg
    19081 gcaattaagc tgcatctcca tcttctttgt ctgccactgc tttgacagcg taagccacgt
    19141 tgcggactgg cgcttgtagt acagatagca acatagatag aagtccttcg cgatttggca
    19201 attttgccaa tgctgtgatt tcttccaatg aagagacttt accttcgatg ataccgcctt
    19261 tgatttctaa tgcttttgct tctttagcaa attcgtccat gattttcgct ggtgcgacta
    19321 catcttcgtt actgaatgca acagctgtag gtccagtgaa tacttcgtcc atgccttcaa
    19381 ggccagcttt tttagctgca cgtgaaagaa tagaattttt gataactttc atttctacgt
    19441 tagcatcacg aagttgttta cgtaaacgtg ttacttcatc aactgttaat ccgcggtagt
    19501 caacgactac tactgatgca gctgctgtaa atttttcgct tacttcatca acgattgctg
    19561 ctttttttgc gataattgct tcactcattt ttgagtttca cctcctgaat tttgatggaa
    19621 gagttttttg cactgaaaag gcaagtcact tttatagtta ctttgctcaa ccagtattat
    19681 tcatcaccta ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa ggaaactcct
    19741 ttcgctttgc tcaaccagta ttgttcatca tctcttcact tcgttcatag tgattcacaa
    19801 taaaaaactc tatgccaccg cagacataga gggactattg aatatattca ataaacgtcc
    19861 tcggtaggaa attaaggctt gatgccacct actgtcttcg gtacaagtta attattaacc
    19921 tcaactagct tagcatgcta aactagttga gtcaagaatt atttttcagt tggatcaatc
    19981 aacattctaa tatttaatta gaaagaagct tggtcaacat ggataccagg tccaaatgtt
    20041 gttgtgatcg ttacattttt catgtattga cctttagctg ctgatggttt agcttttagc
    20101 aatacatcat ggattgctgc aaagttttca actaattttt catcgtcaaa tgaaactttt
    20161 ccgattggta catggatatt tccagctttg tccacacggt aagttacttt accagctttc
    20221 acttcgttta ctgcttttgt tacgtccatt gtaactgtac cagttttagg gtttggcatt
    20281 aagccttttg gtcctaatac tcgtcctaga cgacctacag tagccatcat atcaggtgtt
    20341 gcaacaacta catcaaagtc aaaccatccg ttttggattt tttgaaccat gtcgtcgtca
    20401 cctacgaaat ctgcaccagc tgcttcagct tcttttgctt tgtctccttt agcaaaaact
    20461 aaaacagttt gtgttttacc agttccgttt ggtaatacaa cagcaccacg gatttgttgg
    20521 tctgcttttt tagggtctac gttcaaacgg tatgcaactt caactgttgc atcaaatttt
    20581 gcaatgtttg tttcttttgc taaagctact gcttcggcaa caggataaac tttacttgaa
    20641 tcaacttttt ttaatgcatc ttgcattttt ttgctctttt tagccatttt ggttcctcct
    20701 tgattgtggt tataacggtt gtacctccca cgtgttctat atctttcaat atagagccaa
    20761 ctgagaagct gctgtcttag ggtgtataga ttattctaca gtgatcccca tgcttcgtgc
    20821 agttccttca accatgcgca ttgctgcttc aacgttagct gcgtttaggt cttccatttt
    20881 taattcagcg atttctctta cttgatcgct agaaactttc gcaactttat ttttgtttgg
    20941 ttcgcctgaa cctttttcga ttttagctgc ttttttcaat agaacagcag ctggcggtgt
    21001 ttttgtaacg aatgtgaatg aacggtcttc gtatacagaa ataacaaccg gaatgatcaa
    21061 acctgcttga tcagctgtac gagcgttgaa ttcttttgtg aatcccatga tattgatacc
    21121 cgcttgacct agtgcaggac ctactggtgg agctggagtt gctttacctg caggaatttg
    21181 caatttaacg attttttcta ctttctttgc cacgagacat acctccttaa gtccgtgatg
    21241 tggttaattg gagatgcata tttctcctcc cacgttttat ctgatctgaa aaaatccaga
    21301 caaataatgt gcaaaacaat gcacactggt acagtatagc aaatttattt gttttttcaa
    21361 gatttttttc tgctattttt acattttgct tgaataaacg ctaaatttat tctaaaaaaa
    21421 caacaatttt tcgttctcat ttcttctttt atgttacact tgcgctagca tggttcaaca
    21481 gatgaaagca aacaataaca cggtttttta gacccttgta caaagattcc tacataacaa
    21541 aatgaggtgg agaaaatgaa agatttaagg tatagaagtg tctttgatat tattggacct
    21601 gtgatgattg gtccaagtag ctctcacact gccggagctg ctagaatcgg gaaaatcata
    21661 cgtagtatat ttggcgaaca gcctgattct gtagatattt atctatatga atcatttgca
    21721 aaaacctatc gggggcacgg aacagatatt gcattagttg gcggcctgtt agggatggaa
    21781 cccgatgatg aacgtttagc tgattctttg aaattggcat atgaagccgg tatggaagtc
    21841 ttgtttgttc ctaagagtga aaaagccgaa catccaaact ctgtaaagat gcttctaaaa
    21901 aaaggaacac gaaagctttc agtcactggc atttcgatcg gcggtggcaa tatccagatt
    21961 tctgaattga atggattcaa aatttcttta agcatgggta ctcctacctt gatcgttgtc
    22021 caccaagatg ttccaggtat gattgctaaa gtgactagta tactttctga gaccaatacg
    22081 aatatcggca ctatgacagt gacacgggaa tcaaaaggtg aaaatgccat tatgataatt
    22141 gaagtagatg atcctcaagt agaagaaacg gtaaaaagac tcaaacagct gccaaatatc
    22201 gacagcgtaa attatttcga ttaaggagct aaaaaaatgt ttttatcgat tgaagaatta
    22261 gtagatcaag caaaagctta tccaagtgta gcagaattga tgattgcagt tgaaatggaa
    22321 atgagtggcc gttcccgtag tcacatcatc gagatcatgg aaaaaaacct ctcagtaatg
    22381 gaacaatcaa tcaacgaggg aaaagaaggt gttacctccg tcacaggaat taccggtggt
    22441 gatgcaaaaa aattagatga ttatttaaat gaggcagact ttttaagtgg agaaacaatc
    22501 ttaaaagctg tacgcaatgc tgtagctgtt aatgaagtca atgcaaaaat ggggttgatt
    22561 tgtgctacac cgactgccgg aagcgcgggt gttgttcctg gtgtgctgat ggctgtccga
    22621 gatcggcttg atttgacaca tgaacagcaa ttggaattct tatttaccgc tggcgctttc
    22681 gggttagtca tcgcaaacaa cgcttctatc agtggtgcag aagggggatg tcaagcagaa
    22741 gttggttctg caagtgcaat ggcagcagca gctttggttt gtgcaaaaaa tgggactcct
    22801 catcaagcag cacaagcagt agccattaca ttgaaaaata tgatgggact gatttgcgat
    22861 cccgttgcag gcttagttga agtcccgtgt gtcaaacgta atgctttagg ctcttctcaa
    22921 gcttttatct ctgctgatat ggcattagcg ggtatcgaaa gtgtcattcc gccggatgaa
    22981 gtcgttgctg caatgtacca agtcggacgt caaatgccgt cgattttcaa agaaacagca
    23041 gaaggtggct tagctgttac gccaactgct aaacgacttg cggaagagat tttaaataag
    23101 agggcgtgat aaagctcctt aatttcgagt attaagagat tagttatctt cataacaaaa
    23161 aacgaagaga ggactaattg cccttctctt cgttttttat taatccatca atttcgtata
    23221 ttttacttca tcgatcgttt ttgttcctgc aagttgcatc gtgatcgaca attctttgtt
    23281 caggtgttca aaaacagatg ttactccttc tgccccacct agattcaacc cataaatcac
    23341 tggtcggcca atcgcaacta agtcagcacc acttgccaga gctttgaaga catgctctcc
    23401 tcgacggaca ccagagtcga aaatcactgg tacacgtttg ttgactactt cggcaatttt
    23461 tggcaatact tcaaaggacg caggacctcc atctagttga cgtccaccat gattggatac
    23521 ccagatacca tctgcccctg cagagatagc gattactgca tcttctggcg attgtattcc
    23581 ttttacgata actggaagat gggtgatttc tttgattgtt ttgatgtcat ctggtgtcaa
    23641 gccttgtttt gcagctgcat aaatttctgc gattcctttt ccttcgccat taccacttgc
    23701 actttgttcg ctatacgcag ctaagttagg cattggtaat ggaaattgga attgattgat
    23761 gacgtcttct tcacggtaac caccgagcgt tgaatctgcc gttaaaatga tagctttagc
    23821 ccctgcttct actgctttgt tcaaaatgaa ctcattgaat ctgtcgtctt tactcatata
    23881 taattggaaa aactgcggtg cgcctggcgc tgcatcggct gcgtctttga ttgtcgtatt
    23941 tgcataagtg ctgatggaaa agattgatcc agcagcagct actcctttag ctgtatcagc
    24001 ttcccctttt tcatgtgcca atccttgggc cgctgatggc gcttggataa tcggtgtatc
    24061 taattcaatt ccgaaaacag atgtatgcaa atcggctgag tcgattccgc gtaatatccg
    24121 tggcatgatt ttctttgtat taaaagaagt cgtattttct ttcatcgtcc actcatcttc
    24181 agaaccaccg cgaatatagc caaaagctcc tgcttccatg cgctctttta cacgaccttc
    24241 tagttaggca atgtttacaa tatcgattgg atgttcttca ttacttgtat gataagtcat
    24301 agtagatcct tctttcatct tcttgattac atataaatca tactaaactt acaaaaagta
    24361 agcaaacatt tactctcaga tacttttatc gctattcttt ccaaataaat cgacttacaa
    24421 gctgatctac ttcttggttt tcatgaagca tactatgaga ggcattttca cctgtcacta
    24481 ctttttcctc tatttcattt ccgcgttgct tcaacaacgc atagaccgct aatgcactgt
    24541 ttaacggtac tgttccatca ctcaagtctg tttcatctag ttgaccagca atcaagaaaa
    24601 atcgagtggt tataggaata ttaccgatca tttgctggta atcttgatag cggttagaaa
    24661 cgacagcagg cccctttgct aactcatctg tcagagactg cgcactatca tcgacaaaat
    24721 cattaaaagg cgcgccaatt gctgttaatg tctgaacttg tggcaatgac gtatcctgtc
    24781 catacgttcc taaatagcgt agtgcactaa ctcctcccat ggaatgcccc actatgttga
    24841 ctttatctat gccgtagttt tctttcagat acaaaagaca agttttgatc cattctgctt
    24901 gattccactc gttattttta ttatcttcaa acaatacttg gaccatcgga ttgtctgctt
    24961 ttccagacaa atctcctgtt gcttgtattt gtccatctgt tcctactgtt aaaaccaatt
    25021 cttttactgc ttgctgattt ttttccaaac gccgcaacat ccctttaaag gaattttctg
    25081 tcccgctgta gccgtggaaa aaaagtgttg gaatagtggt tttttctatc actttactgg
    25141 attctgccgt tttttcttta tcagaaactt cagtagtatt tatcttcgtc gtctggcaac
    25201 cacttaacgc aaacaaactg ataaggacta aagctgctgc agttgcacgt ttgcataaat
    25261 ttttgattgg ttctgtcata cacctcttcc tttccatata gaaaaaagaa agacttgaaa
    25321 ggccaatcat atgatcgaca tttcaagtct ctttattaat cgattttatc tacttgatcg
    25381 aaatcaagtt ctgtactggt ttcacgtcca aacatatcga tgttgacttt caatttttct
    25441 ttttctccgt caacttctgt tacttgtcct tctaatccag caaatgcgcc ttcgatgatt
    25501 ttcacgactt cgccaacttc tacttctaga tcattttgtc gcaggctcat accgatagaa
    25561 cgcaagatat gattgacttc ttctggaagt aatggtgctg gtttgcttcc ggctccgtgt
    25621 gaaccaacga atcctgtcac accaggtgtg ttgcggacaa cataccatga agcgtctgtc
    25681 atgatcattt caacaagtac gtatcctggg aatgttttat gaacgatttc tttttctttc
    25741 ccatttttga cttcagtttc tttttcttca ggaacaacga cacggaaaat aaagtcggcc
    25801 attttcatac tttgcgcacg agattcgata tttgctttta ctttgttttc atatcctgaa
    25861 tatgtatgca agacatacca gtttttttca aaagattcca ttgaaggctc ctcatttcat
    25921 tgtaaaataa aaaaacctca gtacacccga agtttttctt tcgcttcatt atagcactaa
    25981 aagtacgttg tggctagcga gttattttaa aatccagcca aataagcttt ggatggctgt
    26041 atccattacg taaaacatca atccgaaaat aatagaagtt tcgatgacta ctaacgtatc
    26101 tttacggagt tgttttctgc ttggccaagt tacttgtttg atttcatctt ttacgctttt
    26161 tagaaatttc atgagacaac ctcccttgct actgtttatt tcgtttcttt atgtaatgta
    26221 tattgattac aatatttaca aaacttattg atttccaaac gttcgccgct ttttccttca
    26281 ctgactgatt ttgtgtagtt gcgagaccca caaacggaac aagcgagtgc tgcttttttc
    26341 tttgccattt acttctgccc ccttgctgag aatcagactt atacttcata acagtatcac
    26401 gtaacttttt acttgtcaat ttgtgaaata aactatctat tgttttggat aaaattatga
    26461 tatgattaat ttgaaattaa gctaaaatga gacaattgga ggtatagcga tgctattaaa
    26521 aacacttgta tataagaaaa aagatctgac aaccgttaca gagactagta cattagaaga
    26581 agcattaaaa attttagaaa cttccggata ccgttgtgtg ccgatcttag attcttcagg
    26641 taatattttc cgtggaaaca tttacaaaat gcatatctat cgtcataaag caaatggcgg
    26701 tgatatgacg cttcctgtca ctcatctttt gaaaaacgct acaaagttca tttacctgga
    26761 tacttctttc ttcaaagtat tctttacgat caaagagctt ccttatattg ctgtccttga
    26821 cagtgaaaat catttttatg gtattttgac acatagtacg ttgcttaata tgctctctca
    26881 atcttggagc gtagaacaag gaagctatgt actgacaatt gcttccacag gaaagcaagg
    26941 agatttggct gctattgcaa aaatcatttc aaaacacagc agcattgcca gctgtattac
    27001 tcttgatatc gaaaaagacg aatacattcg acgtacattg atcacgctac cggctaacac
    27061 tgaagcgaaa gtatgtgaaa cgatcgtcaa ccacttagag aaaaagaatt ataaagtggt
    27121 agaaattgaa aatttgcaaa accaagaagc ataatcaaaa agcgaaaaaa gaaaacagct
    27181 aatctgcttt cttttttcgc tttttataat tagacacgct tttcaaaatc aagcgcgcga
    27241 tctaaataat acatcaatgg tgctgaaaca gccatgacga taaactcact taatgcaagc
    27301 gtgccatagg ttgtccaaaa tgcttggcca cctgttggaa ctaacatata agcaatcagt
    27361 cccatgctga tggtaaagaa gatcgtgttc aatactaaac gcatctttac atttttcact
    27421 ttgttttgca ataaagctgt caggcctaaa gaaagcaatg tttggccgcc accaaaaacc
    27481 acatccatga taccaaaacc aaagaaggca ttatagacga tgaccccgcc taaaactccc
    27541 caaagcaatt tacggttaaa gacaactaaa tgattcaaac tttctgaaat acgaaactgg
    27601 atcggccctg aggatacagg cgcaactaaa atagttaacg ctagataaag agccatgatg
    27661 atgccattta caacgattac ggataaccgt tgtctgcttt tcgttttttg cattttctct
    27721 cctcttttct cctagttttt ttaacgcggg atggttgatg aaccgcggtt atgtgaaaca
    27781 aacaataatt ataacagact tttcatttat ttcaatccgt cttatctgct tttaaaaaag
    27841 caaaagctta aacaatgtaa acgtttgcta aaaatcgtgg gatatggtat aaagtaagtg
    27901 taaggggtca taagaattcc cagtaaagca gacgaagtag gatctgcaag aacagaattg
    27961 ttgtgaaact taaataaata aaaaaggatg tgggaatttg agcaacctct tactagagaa
    28021 cggtctagca agcagctgaa gatctaagtg gacatgccga accttggaac ttctccttac
    28081 gtaagtaatg gatcatgcgc ttgctaggct tttttgtgtt tttcggtctt cggacctttt
    28141 tcattttcct agacttctgc tataataacc aagaattaat tttgtaacag atttttttga
    28201 aaaaacaagt ttctttcatt caaaaaatag aaaaagcttg ctaattattg gttaaccagc
    28261 aaacaggagt attaaaatat cttgttttat tgaatagcag gaggaaatca tgaaattcca
    28321 ttatcatata ttgatcaatc catcggcagg cagcggaaat ggctataaag tagcagaaag
    28381 aattttaccg gtattaaaaa ataagcatat tgactataca cttcattata gtgaatacaa
    28441 aggacacgat gctgagattg ctgaaacttt ggcaaaagaa acattgattc cttggataga
    28501 agaagaacag aaaacaatcg atactttccc ccttttgatc atcgtaggag gtgacggcac
    28561 cttacatcag gtacttgata ctttctatca gatggaagta gaattcccag ttgcttatat
    28621 tccagcaggt tctggagatg attttgctcg aggggcaggc cttccaaaaa atcccaaaaa
    28681 aggacttcag ctgattttag cagctcaatc accagaaaaa gtccacataa tggcttatga
    28741 agaaaaaata agtgaaaaaa aaggaatagc tgttaataat ttcggtatag gattagatgc
    28801 agctatcgtc catgcgacaa atcattcttc cacaaaaaag cgattgaaca aatacaactt
    28861 aggatcattt tcgtatttat tctctattct ccgtgcttta ttcactcaaa aaggatttcc
    28921 aattctggta gatgcaggag gaaaacaatt aagtttttca aacgcatttt tatgtacagc
    28981 aacgaagcat cctttttttg gcggaggaat cgcgattgtt ccaactgctg atgcgtctaa
    29041 accagtcatt gattttgtcg tggtcgaacg aatcaatttc tttaaaatct tgtggctcat
    29101 tcttttactt atacagaaaa aacaattgaa atcgaaatat ttccatcact acacaacaaa
    29161 cagattacgt atcgtttcaa caacaccgca acatggtcaa gaagatggag aagaaatgga
    29221 aaaacgacct tttgatatca caataaccac aactaaacag ttgttttggt gttaataaaa
    29281 gaggttttgt cagaagctac atctggaaca ccgtttattc ggaaaaaagg actgtgacac
    29341 gacttttgtc acagtccttt ttttactatg cagttaattc tttgcccaat tgggttgcca
    29401 tcgatagttt ctttctttac ccaaaataaa ttggatcatt gaaaacaccg cagcaaaagc
    29461 aactaagaca caaccattga accaaaaaga ttctgcaaat gtttgattgt aactgagtgt
    29521 ttcactgatc aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg ccgctatttt
    29581 gctaaccatt tctggcaagt attctgctgg tacaaacacc ccaccgataa agcaactgcc
    29641 tgtgataaaa atatctttga tcccaccgct ggtttctgag ttttttacca atgaagtgat
    29701 acaagccgaa aagctaatga tcggcaataa aaacaagaaa gtatttaaaa tatgatacca
    29761 taccgttata tcccacgagg tgtggatcac taacagaacc attagaagta agaagccact
    29821 gaacaacagc agcgagtaac taatcaaact aaaggttact ttgcgattca agtttcgttt
    29881 ggtaatgggc gcactctggt ttcgtttact gatctctttg cgattaaatg ctagattgat
    29941 gacgctgaaa ccgctaaaaa tggatagaaa caacccataa gacaagaaat taaaggttgt
    30001 gcccgtcaac agctttttca tccgctgact atatcccgca cttagctcaa cttctccagt
    30061 aaccgagagt gccatttgtg tctgttccat agctgcatcg attgttttat cttcttcttg
    30121 aaacagaatc agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg ttttagaaaa
    30181 ggcatctggg cgactctgta tcttgatcgt cggcatcttt cctacttcga cttgttcggt
    30241 aaagtctgca ggcagatgta agataatgtt tacacgatta aaataaagag catcatcgat
    30301 ttctttttgt gaggttccat ccaatgtaac gactgtttcc ttgtcattca aatatttacg
    30361 aaggccatcg actacaggcg actcttcttc actaatcaag gcgattttcg catcttgcag
    30421 agaggtattt tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa cagtgataat
    30481 tacacctatc agtaaattcg atttatttgc tttaagcaat gtccaaatcg ttttataaag
    30541 cgtcataacg cacccctctt tccatccaag catttcctaa cgcaaaaagg aaaataaatc
    30601 cgatcaacca tagaagattg gtataaaaac tagccaaatc agtgtagaaa taaagtttat
    30661 acaaactttc gctgatcaag ttgacgagat ttaattgacc aacaatcgga atatgaagat
    30721 caatccaata cttcacttgt tccgacccca tcataccagc aaaaaagctc atcgcaatcg
    30781 tcactgaaat caagatgcta tccttttgca ccagattcat ttttggtatc aaattaccaa
    30841 ccaatgtacc taacaaaatc gcacacaacg accccagagc ggtaacaatc atcaaccatt
    30901 gccaacgttc accaaagtct acttgataga taaaacgaaa gacagctaac ataatataaa
    30961 tctctgtcaa aacaatcgta aagcttgctg ccaaattagc gattgaaact aacgattgct
    31021 ttctaggaat caaggacaag cgaataccat ttgacgattg atttgcttgc tgatcctgta
    31081 cattgcggac cccccacatc gtcccgtaga gaatcgccat tccaatcaga gtgaagaaat
    31141 aaaaactttt gaaactcata ttacgacctg catgaacttc ttgaaaagat acttcttgtt
    31201 caaaaacagt catgatgggc gcgagatcaa cgttctccac ttctgccact ttactcgaaa
    31261 ttataccaat acgctgtaaa aattgatcca tgacgttttt catcaacgtc tgactcattc
    31321 ctgcttgacc cacatggagc gaaacagatg aaccatcaat gagataaaaa ccagccaaat
    31381 catctgcatt tagcttttca ttcgcttcta cttccgatag ttcctgtact tcaaataatg
    31441 gtttatcatt gacttcgact tgttctaaga ctgtggtaaa attttccgaa agcgctgttt
    31501 gatccgcttt gattacaatt ccagtattaa tcgtttcgag attgccacct tgatcaatct
    31561 cgccaaaggc cgccataaac atcaatccta aaagaattgg aaaggccaat gtccaaaaca
    31621 acatggattt atttcgcagc agtatttttg tgcgatattt ataaaaatgc caaaacatta
    31681 tttcccctcc tctagtctct caaatccttg ccagtaatct ctaaaaagac atcattcaag
    31741 gacgcttctt tggttgctat ttttccgtaa ctgatctttt cttcttgtaa gattgtaaga
    31801 atcggcaaca atactgtatt tagttcagta gttgttactt ggagctgacc ttttttataa
    31861 gcgattccat cagaagctat tgttcgctga caacacgttt tctgggagta atgggatgtc
    31921 gatcgtcaaa cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc
    31981 aatcacctgt ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat
    32041 gtaatgtgtt gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa
    32101 aattttattc cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg
    32161 cggcttgtgg gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc
    32221 tcctggacga aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa
    32281 ggctttcctt ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg
    32341 cacacttagt tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac
    32401 agcataattt tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt
    32461 taacaaagaa agcatacagt tgatggcagt cgatttccca ctgccgtttg gtcctaacag
    32521 acctaagact tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc
    32581 atagcgtttg actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc
    32641 ttgttgatct gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac
    32701 acatgacatt tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa
    32761 ctagctttct actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc
    32821 aagttatcta tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa
    32881 agccaaattg gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg
    32941 tcctgtttct gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact
    33001 tgtttttgtt aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg
    33061 cctttgtcgt aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga
    33121 ccgatcttga gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt
    33181 tatcggaaca aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg
    33241 tggaagaacg gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa
    33301 gtgcgatttt acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac
    33361 cattagaaaa tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc
    33421 atgacctcca tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt
    33481 ttccttcttc tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa
    33541 actgcttctt aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca
    33601 aaaccgttgt tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg
    33661 atggcaccag cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac
    33721 gtgttcaaga acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa
    33781 cgatcatttt accaagggag ggacaccaat gataacagta gtcattattg agatgatcca
    33841 tttgtgacga aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc
    33901 attggtcatt gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg
    33961 ctgacggaca tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct
    34021 aaatttccca aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc
    34081 gaagcatttt ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt
    34141 ccttctgtca aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact
    34201 ttcactcaaa tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt
    34261 gaacgtgagc ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca
    34321 gatgccttgt acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag
    34381 ctagatttac gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc
    34441 ttatctaaca caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat
    34501 cttctattca attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag
    34561 ggctgccagg attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta
    34621 acgttttttc tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca
    34681 atttgaattc tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta
    34741 atttcgctaa tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca
    34801 gcttagcata tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta
    34861 tttctcatct ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt
    34921 ccataccaat catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat
    34981 ctttagaaac agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca
    35041 gatctggttc cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt
    35101 ctacaggtag cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg
    35161 gatcatttcc gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca
    35221 ttttatcatt atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg
    35281 ctacacaagt gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag
    35341 tcactgccgt gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca
    35401 aaataaccat cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag
    35461 tgattttatt ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta
    35521 attcatccac tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat
    35581 ctccattgaa tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc
    35641 gataaacggt gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct
    35701 cctgcatcat tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata
    35761 cttggatatc ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca
    35821 agggttcaat ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga
    35881 aatcttttgg cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat
    35941 caccttgccg ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat
    36001 catggagtac ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa
    36061 cttgcatatt cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag
    36121 acatctcatc aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt
    36181 tttcccgtcc attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt
    36241 cgttcattcg tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt
    36301 gggtatattt tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc
    36361 cgttgatcac agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac
    36421 ggattgcttc agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt
    36481 cgatacgccg aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac
    36541 tgagaaggcg ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt
    36601 aaagattagt ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag
    36661 caacctgatc aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct
    36721 gaagcaatgt ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct
    36781 gatgcaagat agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg
    36841 ctagattatc tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat
    36901 tttcttcgat gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact
    36961 gactaccgaa cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc
    37021 gaatcgtctc agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata
    37081 ccgaaggatc agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt
    37141 ctgctgtttt cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat
    37201 ttgcagttgt tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag
    37261 gatgatccac taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac
    37321 ttccggtgac tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca
    37381 aaagcacttt gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga
    37441 aataaggcat ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct
    37501 ggtgctgttt cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat
    37561 aatctgcaag gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt
    37621 tcacgcaact cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct
    37681 ccatgtccta cgaataaaag tgggccatct ttatgttggg caaccgcatc tgctacaaca
    37741 gtttcgatac gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac
    37801 agatctaaat gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg
    37861 atcgactgac cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc
    37921 tctaccggac gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct
    37981 gtactggaat aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt
    38041 tcttcgtaac tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc
    38101 tgattccatt gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg
    38161 tctttctttt attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga
    38221 tgcgtcaatt ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca
    38281 gctgtattcg ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac
    38341 ttttcttcgt tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa
    38401 aagaagtgct cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt
    38461 gagatcaaat gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg
    38521 taagtaatat ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca
    38581 gttgaaaaac ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg
    38641 gcaatatttc cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg
    38701 atcattcctc tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa
    38761 aaaaacgtat caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca
    38821 ctaactttta tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat
    38881 ataacaccct caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt
    38941 tctcttagtt ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata
    39001 taagtctgct ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc
    39061 tttttgattt tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt
    39121 cattgcttca gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg
    39181 attcggataa aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa
    39241 cacttctcca tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat
    39301 atttaaaacc gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca
    39361 ccaaaatgct tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa
    39421 tccaacagca ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc
    39481 gcagccaaca tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac
    39541 gggctgatac atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta
    39601 ttctgtttta accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg
    39661 gtgattattt ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg
    39721 acagcggaac agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa
    39781 catagttccc cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta
    39841 aaaatatgat acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa
    39901 gaattttgcg ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg
    39961 atcgaattgt gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa
    40021 gaaacgttaa gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga
    40081 gggggcgact tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa
    40141 gcaaagaaat tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta
    40201 gacgaagagg ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat
    40261 atggcttttg acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa
    40321 catggtgttg accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat
    40381 ttcccacggc ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt
    40441 ggtggtgtca ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta
    40501 cacggaacca aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac
    40561 aggcataatg cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag
    40621 aagcaatatt gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac
    40681 cgcttgctac tgctggatca aatcctagac gatccattag catcgggatt aaactacctg
    40741 caagattcgc cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa
    40801 aattatgttt ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga
    40861 caacgccggt caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag
    40921 aaatcgcaag tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc
    40981 ctgtaatcag cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt
    41041 gactgattaa agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac
    41101 gtcgagacgc tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc
    41161 ctgaataatc gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga
    41221 ttcctaacaa atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa
    41281 atgtttgtgc cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca
    41341 tcagatcaga aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc
    41401 ctactagctg accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg
    41461 cttcgttctt cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa
    41521 attcagttgt catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt
    41581 cactggcatc gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg
    41641 tattaagcaa gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc
    41701 taggacgcat ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga
    41761 acatatccgc taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg
    41821 taagagattg gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga
    41881 atgcctgaat attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt
    41941 cttgattttc atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc
    42001 ctccatgtct gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta
    42061 aaaacgcaga tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc
    42121 atacatatca tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg
    42181 tgtccttcct gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat
    42241 ccagtactca gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga
    42301 ataatctcgg gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc
    42361 actgactaag gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt
    42421 tgcgtgcgca aatccatgct ttgcaaaaag catcaaacca gatgtatccc gatctagtcg
    42481 cgtcaccaca tggattactt ggttttcgta accttgtttt ttataataag cctttacacg
    42541 attagccatt gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt
    42601 atttactacc agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag
    42661 tacggtttca tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag
    42721 agaaaacaag acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat
    42781 tttcgctaac agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga
    42841 atgatctttc tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt
    42901 tcacacgatg ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta
    42961 ttcgataata aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa
    43021 gataatcttc tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg
    43081 gagaacccaa agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg
    43141 cattgatact aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag
    43201 taggcgtaga aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt
    43261 atacatcggc taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa
    43321 aatgctggtc cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa
    43381 cactttgttc ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc
    43441 aatccgtgta aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac
    43501 aatgactgta gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca
    43561 attctggctc atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt
    43621 ctgtcacttc tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc
    43681 tcctctcttt cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct
    43741 cttccatggg agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct
    43801 tcatccaact gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga
    43861 aaaacgcctt gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca
    43921 agagtccgaa tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga
    43981 tactcgatca ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct
    44041 cgttcttgga gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata
    44101 tcttctacaa actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa
    44161 cgtcccattg gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact
    44221 ctcccagtga caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc
    44281 ccccgcagtt tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc
    44341 caatctcgtt ccatagctga tacctctact tcattaacat tcattgttta ttctaccata
    44401 tatgcaaagt tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa
    44461 aatagacaaa aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg
    44521 ctgacaaagc aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc
    44581 cgtgtacaga ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt
    44641 ggcctacgga tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa
    44701 aaagggcgtc tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc
    44761 gaaaaacgtc tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat
    44821 cctgcttcat taatccaaac cggaaagctt acaacaaaac gggctgaatt ccctattgaa
    44881 gaaggacttt tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa
    44941 ctagaggtaa ggggatgcat ctgtgggaaa aattgctttt ataaattttt taaaacgttt
    45001 ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta
    45061 gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc tgttaagtta
    45121 taggatgtga aagaatctag tcactagttg tatagaatct attttggggg agattcatat
    45181 gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa tggaacaaca
    45241 gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga tgcaatttcg
    45301 ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa aaggaatttc
    45361 tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg cagcattgga
    45421 ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga tcaaattgca
    45481 agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat ccatatttga
    45541 agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact tcgttaaaga
    45601 agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac acccttctgc
    45661 agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac
    45721 aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat atcaagtttt
    45781 taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta gccatttaca
    45841 cttgctttct aattaaaaaa atacgtcgac tcccttacaa gagagccgac gtattttttt
    45901 cgtttctatt cgagaaaatc aaaaatagaa agccttataa acgatcgatc aattcttcta
    45961 attcatttag acgttgttca aacatcgaca ttgcatcttc aatatatgca gcctgtgtca
    46021 tatcaactcc tgcttttttc attacttcca caggataatc gctgttacct gcttttaggt
    46081 aagccaagta gttctctagt gcctctggtt cctggtttag tattttcttc gccagtgctg
    46141 aagcagcaga aaagccagtc gagtattgga aaacatagta attataataa aaatgcggta
    46201 tccgtgacca ttcgaattta atttctgggt cttcttcgac tgctggacca taatattttg
    46261 cattcaattt accataacta tcacttaggt attcactagt caatggcaca cctttttcat
    46321 cttccgtatg catgaaatgc tcgaattcag cgaattgtgt ctggcggaaa actgttcctt
    46381 taaacccatc caagtaatgg ttgagtacat aagcccgtac acgagggtct ttttctgttt
    46441 ccaataaata ctccgttagg atattttcat tcgttgtcga agcaatttct gctaaaaaga
    46501 tggagtagtc gccgtacaca taaggctggt tcgaacgagt gaaataacta tgaacactat
    46561 gtcccatttc gtggacaagc gtaaatagct gatccagcgt atcatgccaa ttcaataaaa
    46621 tatatggatt tgtgtcatag cttcccgaag aataagcacc gcttcgtttc cctttatttt
    46681 cgacaacatc gatccaacgt tcagagaatg ctttttctac gatggccatg tattcttcac
    46741 ccattggttt cagtgcttct aaagcttttt cttttgcttc ttcgtacgta aaggtaattg
    46801 gagcttcccc taagaccggt gtataaagat catacatgtg cagtttttcc acttctaata
    46861 aacgtttccg taattccatg tatcgatgta acaaaggcaa atgcttgttt accacgtcta
    46921 ccaaagtatc gtatacactt tcaggaatat gattattgct caaagacgct tctctggcag
    46981 agctgtaatt acggactttc gctttaaaat tatgtccttt tatatgtgtg cctaaagtag
    47041 aagcaaatgt atttctaaat tgttcgtaaa cactgtacaa tcccttaaat gctgcttcgc
    47101 gcacccttcg atccgtgctt tctagcaact gaccatacac gccatgagat aattggacta
    47161 tttcaccatt ttctccttca atcgttggaa aaactagatc tgcattattc aaaacagcaa
    47221 atgtatcact tgaagcatca aagatttcac ctgctccagc aagaagagat tcctgctcag
    47281 cagataagac atgggctcga ttatctacta tttgctggat ataatggcga tagacttcca
    47341 attttggttc ttctttgaaa tattgccaaa tctggtcatc tgacaattgc aatatttctg
    47401 gttcaaacca ggaaacggct tcactaactt tcgaaaacag actgcttgct cttgcataaa
    47461 gcgcttggta atctgtattt ccagtgtctt gatcgttttt aaggtgcgca tatacataaa
    47521 tgacttcagt ttggcgataa accctcaata cgaattcaat cgcatttaaa aattgagaag
    47581 cgccttgatc aagtgttcct ttgtgttttt cagattgttt taactcttct gataattcca
    47641 aatatttctc atcaaactct tggtcgcttg aaaagatctt ggtcaagtcc caagttaaat
    47701 tttcaggcaa ttcttctcgt tttggcaact gctttacttc catgaaatcc actccttttc
    47761 ttcccttagc tctatctatc aaaatagatc atagtctaac atccacatgt cacagcatct
    47821 aacttttcta ctcattcatg tgtctgcttc catcaatttt agcataacta aatcttgttt
    47881 tacatagaaa accgattcct agcctatttt tatacttact attttttcaa agaagacaag
    47941 tgtatgcatt cgttaaaaaa ctgctgatag accatctctt catcaatcat aggaaaaagc
    48001 cattcccttt tatgttcttt tatttggcaa agccatgagt tgaaatcagg aactttctgg
    48061 ttttccaagg cttcttcgta caacattcga taaagaaaaa cctgttcttg taaataaaag
    48121 aaaaaccgac ttttttgata gatccacgat gatagatgca aaagatgttt atgacgcaag
    48181 tagcattgtt cttgtatgga tactgtattc ttttgcttgt gaataagttg gcgtgtcagc
    48241 catttcttcc catcttcact cactttttct atttttttca tttggttcat tttaatttcg
    48301 ttactagtga atatttcttt aaggtcccta ctagaaaaag tccagcagct actgcaaaag
    48361 gaaatatgcc ctgatacagt ctctgtaata tgatgataca aataaagctt ttgtttcttg
    48421 agatcagctt tccagcaatg cactcctctt tttctattat agtagcagca acttttttct
    48481 atcaatgaga attgcgattg tccaagaaaa gaattaccaa gtatccacca tacttggtaa
    48541 ctgttattca aataattttg ggttctttca ataaatctct ggtgagacag tctagaacat
    48601 tggatttcta ttgctagatt cgccaacaaa atatccggcc tttgccgcaa cctcggcaaa
    48661 taggcttcca aaaatactgg ttctactgac tgtcccagcc aatccatgaa gcattccttt
    48721 aactgtaagt gctcttttga ttctctttca ctaaagaccg aacagttatc tgatttttga
    48781 tgggcaaaat gagcacattt tttctttccc tttttcaaaa tcactctttt tcgacagcca
    48841 cgacaataat atttctttcc tttttctgca tgtttagcaa aacagcactt attatattca
    48901 ttcattgcaa gcagcatttt tctccctctg aacttattta cgtctaagaa tttatttaca
    48961 cgaaaaaaga gggcgtgaaa aagctattct gcatcaagta ataagaacag ctaaacaaaa
    49021 atagttccca atatttttcg tttggttggg cttgttaccg cagatgcaag cttttgaaca
    49081 catttatacg aaaaaaagac tgcgacacaa tttttgtcac agtctcttac tataatgctg
    49141 atttatacaa aataaaattt cgttcgctcg atagcattac gcgccatcag acattgtcca
    49201 tattcattca atgtttctgg tgtacgatcg ctcttttcag caaactccaa aattcgagca
    49261 atctgattct ctacatccgc ttggttgaga ttttccatcc agaagtgaac gactaggtaa
    49321 tagcgctctt cgaataaata caaatcagtc caagcagact ctagttcgac atctgttgca
    49381 agttggatca cttgttcaaa atcttcaaaa gcaaaagttg cttgtctttc ctgttcttct
    49441 agggcgcttc cagataattc ttcctccgga tcgctgccaa taatctgctg acgaagaaag
    49501 tcactcacat cttctgaatc aacagtagaa tcttctgatt gagaaatttc ttcatccgga
    49561 agatttttac tgataaacag ttctaatccg tctccttttg gcaaaacttg gaaagtgaca
    49621 gcttcgcttc ctcggaactc ctcttcgata tcaacttctt ccaaaatact ataaaagaaa
    49681 ttttcgattt ctttgtgatt gccaagtaaa tccaaaaatg aaacgccccg ttcctctaaa
    49741 tcttcatgac caattagtac acggatcgta ttttcattaa tatgttccat ttccattttg
    49801 ctacacctca ctttgccaca atgtttaata tagattcatt gtagcgaaat cagcttaaat
    49861 gtaaagaaaa aaaatctcgc tgttaagcga atttaataaa accagcggat ttttcaacct
    49921 ttcaagctat cttatttaac tataacattt ttggcagacg aatactatat agccgacttt
    49981 tcagctaatt ttccctctct atttttcgtg caatcatcat cttctactac ctttttatac
    50041 atctaatcaa gaatactttt tgtaaaaaca aaaaaacttg gaaaaactat ctttgtaata
    50101 gt
//
LOCUS       DAZKMK010000008        47757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000008 DAZKMK010000000
VERSION     DAZKMK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.008"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACG8V1_000523"
     CDS             complement(38..1189)
                     /locus_tag="ACG8V1_000523"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0328641.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACG8V1_000524"
     CDS             complement(1202..1639)
                     /locus_tag="ACG8V1_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0328642.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACG8V1_000525"
     CDS             complement(1654..3420)
                     /locus_tag="ACG8V1_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0328643.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACG8V1_000526"
     CDS             3684..4361
                     /locus_tag="ACG8V1_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0328644.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACG8V1_000527"
     CDS             4364..5491
                     /locus_tag="ACG8V1_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328645.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACG8V1_000528"
     CDS             complement(5691..6131)
                     /locus_tag="ACG8V1_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0328646.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACG8V1_000529"
     CDS             complement(6142..8175)
                     /locus_tag="ACG8V1_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0328647.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACG8V1_000530"
     CDS             complement(8177..9880)
                     /locus_tag="ACG8V1_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0328648.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8V1_000531"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8V1_000531"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0328649.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACG8V1_000532"
     CDS             complement(10963..11988)
                     /locus_tag="ACG8V1_000532"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0328650.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACG8V1_000533"
     CDS             complement(12197..13066)
                     /locus_tag="ACG8V1_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0328651.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACG8V1_000534"
     CDS             complement(13525..15417)
                     /locus_tag="ACG8V1_000534"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328652.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACG8V1_000535"
     CDS             complement(15414..17141)
                     /locus_tag="ACG8V1_000535"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328653.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACG8V1_000536"
     CDS             complement(17157..17807)
                     /locus_tag="ACG8V1_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0328654.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACG8V1_000537"
     CDS             18011..18307
                     /locus_tag="ACG8V1_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0328655.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACG8V1_000538"
     CDS             18479..19099
                     /locus_tag="ACG8V1_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0328656.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACG8V1_000539"
     CDS             complement(19191..20357)
                     /locus_tag="ACG8V1_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0328657.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACG8V1_000540"
     CDS             complement(20369..20722)
                     /locus_tag="ACG8V1_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0328658.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACG8V1_000541"
     CDS             20850..21872
                     /locus_tag="ACG8V1_000541"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0328659.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACG8V1_000542"
     CDS             21891..22052
                     /locus_tag="ACG8V1_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328660.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACG8V1_000543"
     CDS             complement(22311..22745)
                     /locus_tag="ACG8V1_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328661.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACG8V1_000544"
     CDS             complement(22855..24714)
                     /locus_tag="ACG8V1_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0328662.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACG8V1_000545"
     CDS             24715..24855
                     /locus_tag="ACG8V1_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0328663.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACG8V1_000546"
     CDS             complement(24832..25992)
                     /locus_tag="ACG8V1_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0328664.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG8V1_000547"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG8V1_000547"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0328665.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACG8V1_000548"
     CDS             complement(27739..28437)
                     /locus_tag="ACG8V1_000548"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0328666.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACG8V1_000549"
     CDS             complement(28394..30055)
                     /locus_tag="ACG8V1_000549"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0328667.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACG8V1_000550"
     CDS             30273..31673
                     /locus_tag="ACG8V1_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0328668.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACG8V1_000551"
     CDS             complement(31925..33004)
                     /locus_tag="ACG8V1_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0328669.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACG8V1_000552"
     CDS             complement(33090..34049)
                     /locus_tag="ACG8V1_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0328670.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACG8V1_000553"
     CDS             complement(34061..35572)
                     /locus_tag="ACG8V1_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0328671.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACG8V1_000554"
     CDS             complement(35594..36157)
                     /locus_tag="ACG8V1_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0328672.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACG8V1_000555"
     CDS             complement(36163..37026)
                     /locus_tag="ACG8V1_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0328673.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACG8V1_000556"
     CDS             complement(37040..37936)
                     /locus_tag="ACG8V1_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0328674.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACG8V1_000557"
     CDS             complement(38111..39400)
                     /locus_tag="ACG8V1_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0328675.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACG8V1_000558"
     CDS             complement(39372..40889)
                     /locus_tag="ACG8V1_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0328676.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ACG8V1_000559"
     CDS             41087..41977
                     /locus_tag="ACG8V1_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0328677.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACG8V1_000560"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACG8V1_000560"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0328678.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ACG8V1_000561"
     CDS             complement(42886..44358)
                     /locus_tag="ACG8V1_000561"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0328679.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="ACG8V1_000562"
     CDS             complement(44995..45357)
                     /locus_tag="ACG8V1_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0328680.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="ACG8V1_000563"
     CDS             45580..46332
                     /locus_tag="ACG8V1_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0328681.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="ACG8V1_000564"
     CDS             complement(46472..47395)
                     /locus_tag="ACG8V1_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0328682.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="ACG8V1_000565"
     CDS             complement(47410..47682)
                     /locus_tag="ACG8V1_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328683.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13896 a         9933 c         8250 g        15678 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgcc ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgt
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt atcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaag
//
LOCUS       DAZKMK010000009        45769 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000009 DAZKMK010000000
VERSION     DAZKMK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.009"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACG8V1_000566"
     CDS             <1..730
                     /locus_tag="ACG8V1_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0328684.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            788..2827
                     /locus_tag="ACG8V1_000567"
     CDS             788..2827
                     /locus_tag="ACG8V1_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0328685.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2936..4087)
                     /locus_tag="ACG8V1_000568"
     CDS             complement(2936..4087)
                     /locus_tag="ACG8V1_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0328686.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4241..4771)
                     /locus_tag="ACG8V1_000569"
     CDS             complement(4241..4771)
                     /locus_tag="ACG8V1_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0328687.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4870..5220)
                     /locus_tag="ACG8V1_000570"
     CDS             complement(4870..5220)
                     /locus_tag="ACG8V1_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0328688.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG8V1_000571"
     CDS             complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG8V1_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0328689.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG8V1_000572"
     CDS             complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG8V1_000572"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0328690.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7623..8285)
                     /locus_tag="ACG8V1_000573"
     CDS             complement(7623..8285)
                     /locus_tag="ACG8V1_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0328691.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8469..10115
                     /locus_tag="ACG8V1_000574"
     CDS             8469..10115
                     /locus_tag="ACG8V1_000574"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0328692.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG8V1_000575"
     CDS             10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG8V1_000575"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0328693.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11296..12849)
                     /locus_tag="ACG8V1_000576"
     CDS             complement(11296..12849)
                     /locus_tag="ACG8V1_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0328694.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12997..14166
                     /locus_tag="ACG8V1_000577"
     CDS             12997..14166
                     /locus_tag="ACG8V1_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0328695.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14292..15371)
                     /locus_tag="ACG8V1_000578"
     CDS             complement(14292..15371)
                     /locus_tag="ACG8V1_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0328696.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15480..15920)
                     /locus_tag="ACG8V1_000579"
     CDS             complement(15480..15920)
                     /locus_tag="ACG8V1_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0328697.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(15977..16387)
                     /locus_tag="ACG8V1_000580"
     CDS             complement(15977..16387)
                     /locus_tag="ACG8V1_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0328698.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16568..17002)
                     /locus_tag="ACG8V1_000581"
     CDS             complement(16568..17002)
                     /locus_tag="ACG8V1_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0328699.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17102..17950)
                     /locus_tag="ACG8V1_000582"
     CDS             complement(17102..17950)
                     /locus_tag="ACG8V1_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0328700.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18339..20105
                     /locus_tag="ACG8V1_000583"
     CDS             18339..20105
                     /locus_tag="ACG8V1_000583"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0328701.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20207..21466
                     /locus_tag="ACG8V1_000584"
     CDS             20207..21466
                     /locus_tag="ACG8V1_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0328702.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21613..22914
                     /locus_tag="ACG8V1_000585"
     CDS             21613..22914
                     /locus_tag="ACG8V1_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0328703.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            22917..23768
                     /locus_tag="ACG8V1_000586"
     CDS             22917..23768
                     /locus_tag="ACG8V1_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0328704.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23781..24596
                     /locus_tag="ACG8V1_000587"
     CDS             23781..24596
                     /locus_tag="ACG8V1_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0328705.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24747..25913
                     /locus_tag="ACG8V1_000588"
     CDS             24747..25913
                     /locus_tag="ACG8V1_000588"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0328706.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26143..26790
                     /locus_tag="ACG8V1_000589"
     CDS             26143..26790
                     /locus_tag="ACG8V1_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0328707.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26874..27818
                     /locus_tag="ACG8V1_000590"
     CDS             26874..27818
                     /locus_tag="ACG8V1_000590"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0328708.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            27953..29404
                     /locus_tag="ACG8V1_000591"
     CDS             27953..29404
                     /locus_tag="ACG8V1_000591"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0328709.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29425..30261
                     /locus_tag="ACG8V1_000592"
     CDS             29425..30261
                     /locus_tag="ACG8V1_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0328710.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30261..30920
                     /locus_tag="ACG8V1_000593"
     CDS             30261..30920
                     /locus_tag="ACG8V1_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0328711.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            31046..31735
                     /locus_tag="ACG8V1_000594"
     CDS             31046..31735
                     /locus_tag="ACG8V1_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328712.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31741..32697
                     /locus_tag="ACG8V1_000595"
     CDS             31741..32697
                     /locus_tag="ACG8V1_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0328713.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32741..33253
                     /locus_tag="ACG8V1_000596"
     CDS             32741..33253
                     /locus_tag="ACG8V1_000596"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0328714.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33509..34057)
                     /locus_tag="ACG8V1_000597"
     CDS             complement(33509..34057)
                     /locus_tag="ACG8V1_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0328715.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34146..35327)
                     /locus_tag="ACG8V1_000598"
     CDS             complement(34146..35327)
                     /locus_tag="ACG8V1_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0328716.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35563..37071)
                     /locus_tag="ACG8V1_000599"
     CDS             complement(35563..37071)
                     /locus_tag="ACG8V1_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0328717.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37230..37421
                     /locus_tag="ACG8V1_000600"
     CDS             37230..37421
                     /locus_tag="ACG8V1_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328718.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37624..38541
                     /locus_tag="ACG8V1_000601"
     CDS             37624..38541
                     /locus_tag="ACG8V1_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0328719.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38534..39607
                     /locus_tag="ACG8V1_000602"
     CDS             38534..39607
                     /locus_tag="ACG8V1_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328720.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39755..40273
                     /locus_tag="ACG8V1_000603"
     CDS             39755..40273
                     /locus_tag="ACG8V1_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328721.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40254..40646
                     /locus_tag="ACG8V1_000604"
     CDS             40254..40646
                     /locus_tag="ACG8V1_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0328722.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40722..41657
                     /locus_tag="ACG8V1_000605"
     CDS             40722..41657
                     /locus_tag="ACG8V1_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0328723.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41790..42569
                     /locus_tag="ACG8V1_000606"
     CDS             41790..42569
                     /locus_tag="ACG8V1_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0328724.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42562..42879
                     /locus_tag="ACG8V1_000607"
     CDS             42562..42879
                     /locus_tag="ACG8V1_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0328725.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43123..44733
                     /locus_tag="ACG8V1_000608"
     CDS             43123..44733
                     /locus_tag="ACG8V1_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0328726.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            44890..45738
                     /locus_tag="ACG8V1_000609"
     CDS             44890..45738
                     /locus_tag="ACG8V1_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0328727.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        15181 a         8248 c         8803 g        13537 t
ORIGIN      
        1 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
       61 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
      121 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
      181 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
      241 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
      301 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
      361 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
      421 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
      481 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
      541 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
      601 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
      661 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
      721 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
      781 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
      841 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
      901 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
      961 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
     1021 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
     1081 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
     1141 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     1201 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     1261 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     1321 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     1381 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     1441 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     1501 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     1561 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     1621 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     1681 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     1741 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     1801 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
     1861 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     1921 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     1981 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     2041 tgctcctgga tctacgttta aaataattac agcagcaatc ggtctcgaca acggcactat
     2101 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     2161 atcttttcaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     2221 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     2281 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     2341 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     2401 atatggacag ggcgaacttc taattaatcc tatccagcaa gcagcaatgt attctgtttt
     2461 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     2521 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
     2581 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     2641 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     2701 gtttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     2761 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     2821 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     2881 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     2941 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     3001 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     3061 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     3121 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     3181 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     3241 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     3301 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     3361 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     3421 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     3481 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     3541 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     3601 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     3661 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     3721 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     3781 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     3841 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     3901 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     3961 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     4021 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     4081 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     4141 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     4201 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     4261 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     4321 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     4381 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     4441 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     4501 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     4561 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     4621 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     4681 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     4741 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     4801 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     4861 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     4921 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     4981 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     5041 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     5101 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     5161 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     5221 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     5281 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     5341 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     5401 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     5461 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     5521 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     5581 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     5641 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     5701 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     5761 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     5821 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     5881 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     5941 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     6001 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     6061 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     6121 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     6181 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     6241 tgcactacta gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa
     6301 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     6361 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     6421 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     6481 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     6541 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     6601 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     6661 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     6721 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     6781 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     6841 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     6901 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     6961 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     7021 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     7081 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     7141 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     7201 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     7261 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     7321 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     7381 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     7441 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
     7501 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
     7561 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
     7621 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
     7681 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
     7741 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
     7801 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
     7861 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
     7921 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
     7981 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
     8041 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
     8101 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
     8161 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
     8221 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
     8281 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
     8341 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
     8401 atagcgattt ttattgtata aaattcactt gaatacaaaa actaatatgt tagtttagga
     8461 ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag
     8521 aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg
     8581 tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac
     8641 gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag
     8701 aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa
     8761 gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc
     8821 cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat
     8881 tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata
     8941 taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta
     9001 ttcttgctgc gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct
     9061 ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa
     9121 aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag
     9181 aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag
     9241 ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag
     9301 gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc
     9361 attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa
     9421 cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt
     9481 tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag
     9541 atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc
     9601 caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc
     9661 caaatattcc tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc
     9721 gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg
     9781 tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag
     9841 gaaaaccatt catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg
     9901 atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt
     9961 caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag
    10021 aaatgttcat caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact
    10081 tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga
    10141 agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac
    10201 attgatggat ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga
    10261 agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc
    10321 cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg
    10381 aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg
    10441 ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag cttttatatc
    10501 tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga
    10561 atttgaattg ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc
    10621 ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat
    10681 catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata
    10741 ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg
    10801 catagtagac agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc
    10861 agaaaatgat gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc
    10921 acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc
    10981 cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa
    11041 cgaatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata
    11101 cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga
    11161 aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa
    11221 gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta
    11281 tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc
    11341 ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc
    11401 tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa
    11461 agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga
    11521 ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg
    11581 cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac
    11641 taggtcttca accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg
    11701 attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac
    11761 gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag
    11821 aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc
    11881 aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac
    11941 atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc
    12001 gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat
    12061 tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata
    12121 ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat
    12181 ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt
    12241 tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt
    12301 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
    12361 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc
    12421 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
    12481 ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
    12541 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
    12601 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
    12661 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat
    12721 atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag
    12781 aaaaatatct aattttgtat tgcttttttt cttcaagaga aacgttttaa tcgcttcttg
    12841 agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt
    12901 tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct actatcttat
    12961 ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac catcttaata
    13021 tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca cacagaggag
    13081 atagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat tacctttcag
    13141 gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc
    13201 atcaaagcaa aaattgacta ttcaggagac ttagcaccta tactacaatc agctgttaca
    13261 tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg agaacacttt gactatccaa
    13321 cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt aaaaccagcg
    13381 ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatcattt aacgccgtat
    13441 agtcagcggc agacaattga gcaagattca gctatcgact caggaggaga tcttgttgaa
    13501 gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac agaaaatcca
    13561 gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga aactcaacca
    13621 gagccagaca atgaatcaaa acctgaaata acccctggag aaaaaccaga tgtagaccca
    13681 gaagaaaaac cggatgtgac tccagaacca gatacggatt ctggaaacca aacagtaccg
    13741 gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaactaga ggtagatcca
    13801 gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca aggaatacca
    13861 gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa aaaatcaaat
    13921 ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat atttgatagc
    13981 gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctag tcctgaaacg
    14041 aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga
    14101 atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct
    14161 ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa
    14221 acgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct
    14281 tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact
    14341 ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca
    14401 aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg
    14461 ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta
    14521 atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga
    14581 atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct
    14641 aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtgttt
    14701 aaaagagcga taacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg
    14761 gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag
    14821 aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca
    14881 aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt
    14941 tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga
    15001 tctgcatctt ttggctgatg ctcgtaaaaa tcaataacgg actggatcat atcaaatgtt
    15061 tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt
    15121 gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga
    15181 acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt
    15241 ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca
    15301 ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac
    15361 acgttattca ttatatgatg gaatatgaaa ataagaaaga gaaagagcaa aaagaaaaac
    15421 cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat ctagcagttt
    15481 cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag atgttgcagt
    15541 gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg aataccttct
    15601 gcttgttttt gcaacgcagc accttgagga gtcagcgtaa catttacgat tctttcatca
    15661 cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag taagggagtc
    15721 agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt ctttttcttt
    15781 tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg cttgaggagg
    15841 gaagtgtagt gtctaatgat ttccttagag gcggcatata aaggaaaaca tacttgatta
    15901 actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt atcatagcgg
    15961 atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata tttccagctg
    16021 tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc gtttcttcca
    16081 gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc gtttcacttt
    16141 ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt ttttgctgtt
    16201 tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac aattgttcag
    16261 ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg aatgtacgat
    16321 cgggattatg aacttctcca gagcgtccgc ctgtgttgat cattttcgta gaatacattt
    16381 ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac acaatataat
    16441 tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca tgaaacagac
    16501 gcgctttctc gaatagcaaa catatacaaa aaaatatcag agacaaaaac taatctcatc
    16561 ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaactt acttcacgat
    16621 ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag ttagtcacac
    16681 caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt aatgctttat
    16741 tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt ggatgttcag
    16801 cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat ctcgtgccat
    16861 acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca atcagttgta
    16921 tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt aatacaaaat
    16981 ctatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac agttactaga
    17041 ttcttttatt ataccagtta aagaaaacgc attcaaaaaa atagcggatt tacttaccaa
    17101 ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg ctgctgacaa
    17161 tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa aattcatgat
    17221 cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct tttgcctcta
    17281 gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct tccaaataat
    17341 gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata taagtcatga
    17401 caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt ccaaaaacaa
    17461 acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt
    17521 ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa gagttgtttt
    17581 tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc
    17641 gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa aacaagcttc
    17701 cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt accaacataa
    17761 cgatattcaa cactaaaata actatagctc ggtcaaatcc taaaacagct tccagtaata
    17821 ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac atattgatag
    17881 cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata aattgtctcg
    17941 tttctttcat ttatccactt ccttaattca ttaataatac tcttatctta ttataactta
    18001 cttttcacct taagttaact ttaattaata aaaaaattag gctagtctaa attattatgt
    18061 ttcattcttc tagaatatac ttctacgtta aaaactgtta aaaaactttt actttagaaa
    18121 attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag gaagtagatt
    18181 aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa aaattcggta
    18241 tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgtttct ctatcactta
    18301 tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct gcaatttatc
    18361 atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt attcggcttc
    18421 gtactaggaa aaatgatatc aaaaaagtat atgtgctttg tggagatcca tataccataa
    18481 caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt accaatgttc
    18541 atgattattg ggaaattgaa gtgacgagcg aaacaaggcg cttacagtat gcgttccatg
    18601 ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt ccttatcgag
    18661 aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat caaatcgatc
    18721 gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt cccgaaagat
    18781 ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga agtaaagatc
    18841 cagaccgaga agacttcttc gggggagatt tgcaaggtgt gagggatcat ttggattact
    18901 tgacggacct tggcgtcaat ggcatctatt tttgtcccat atttaaagcc acatccaatc
    18961 ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat aaaaaacttt
    19021 taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc gatgctgtat
    19081 tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat
    19141 cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat cagatgactg
    19201 acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact ccattcatgc
    19261 cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc gcaacttatt
    19321 ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc
    19381 atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc tatatccttg
    19441 gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat gcagtgatga
    19501 attatgcatt cacagattcg attaaagatt attttgcaaa aaagaaaatc acagcttcac
    19561 aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg aacgaaggaa
    19621 cattcaactt attagattct catgatactg ccaggatctt gaccctttgc caaggtaaca
    19681 aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct ccttgtatct
    19741 attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc aaatgtatga
    19801 tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa cttgtttctc
    19861 taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta atagtatatg
    19921 atcgtgaaga taagctctat ttcacacggg aactagaggg acagatcatc tatgtttact
    19981 ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa
    20041 attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt ttgatcatgt
    20101 gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt tgtgcccatt
    20161 ataataaggg tgtaacagca cgttgaatac acaggaggaa atgaagatga aaaaaagaag
    20221 tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat tagcggcttg
    20281 tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt taacagtttc
    20341 cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg aaaaagaaaa
    20401 cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat cattagcgtt
    20461 agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc ggatcggtgc
    20521 gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag cttacgatga
    20581 aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg cagtaatcga
    20641 gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa cgtttaaaga
    20701 tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag gaaaaaatac
    20761 aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg caggttacgg
    20821 tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga ataatgcagg
    20881 tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc caaagggaat
    20941 gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca ataaaactgc
    21001 agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata tcaattatgg
    21061 tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg gcggaggaaa
    21121 aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat ggcttgatta
    21181 tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa
    21241 ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag ctgtgattga
    21301 acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag tttggacagg
    21361 tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag aatccgcaga
    21421 tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa aatgataacc
    21481 gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga gcgagatgga
    21541 aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag caagtttaga
    21601 gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa aatcacgctt
    21661 tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt
    21721 tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt tcatgcattg
    21781 gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt tattttgatt
    21841 gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg tttgaatctt
    21901 ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa tcggacgatg
    21961 aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac acttcccgct
    22021 tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt cactgcattt
    22081 actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt aggggctaaa
    22141 aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc agtcttttcg
    22201 tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg tattctcaca
    22261 gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat cattttctta
    22321 ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat attcaacgat
    22381 agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc ttttgtagat
    22441 atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat tatcttagtg
    22501 cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt acaatcgatt
    22561 tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca gcgcttttca
    22621 aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac acagtatacg
    22681 gggaatttca ataatttctc catgatctat ctgtttaaca atggtggtcc cggaagcgta
    22741 ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact tacgactgga
    22801 acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt catcgttatc
    22861 tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga ttaacaatga
    22921 aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt acttactttt
    22981 ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca agtactgcct
    23041 tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg actttaggaa
    23101 actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat acattgatca
    23161 tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc tatacttata
    23221 gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg
    23281 taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta gaagcgctcg
    23341 ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg aatacttggc
    23401 tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct aaacttgatg
    23461 gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg
    23521 ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta gctaagttcc
    23581 tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt atttctgatg
    23641 tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca ctaccgattt
    23701 gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct ggcggaacaa
    23761 aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc gcattacgta
    23821 cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt ttcttgatat
    23881 ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc aatcagatcc
    23941 aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa caaggaaccc
    24001 aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa caaacgatag
    24061 ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg
    24121 gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat aaatctggta
    24181 tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta cgtacgcccg
    24241 aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca acaatcattt
    24301 tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc attttattcg
    24361 gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt catacattca
    24421 aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca gcagcagttg
    24481 tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg ataactgtct
    24541 tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag
    24601 tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa
    24661 aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat taacgtaata
    24721 gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat caattatttt
    24781 cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg aaacaatacg
    24841 gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag
    24901 agattacaag taacgcttct ttggcactag gagaagccta tatggatcat cgaatcgaga
    24961 tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac agctttttac
    25021 gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca
    25081 aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt tggctagatc
    25141 caactttgac ttattcttgt gcttatttca atacacctga agatacgttg gaacaagcac
    25201 aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc gaaacgttgc
    25261 ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag tataatgtca
    25321 aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa agaataaaag
    25381 aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg
    25441 aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg
    25501 aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct ttgatccatg
    25561 gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat attttccctg
    25621 gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac gatttgcaac
    25681 tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat tggacaaaga
    25741 attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc tatcgaatgt
    25801 gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc gatgtgattc
    25861 aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca taattgaggc
    25921 aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct ccagaagtga
    25981 acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc tttatatttt
    26041 cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt cttcgttata
    26101 cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa aactgttatt
    26161 tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac caatcaaggc
    26221 acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc ggactcctca
    26281 acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc aagtactcag
    26341 tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa atacccagat
    26401 actgcaatta ctagtttgga attagatagt gattggggat cttacttcta caagatagaa
    26461 ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga aaaaatggag
    26521 acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat
    26581 ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa
    26641 gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac tacttattgg
    26701 gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag ccaaacaggc
    26761 gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag agttgatttt
    26821 tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt tacttgagtt
    26881 tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca tctggtgtcc
    26941 actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga gcatttatca
    27001 ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat tttgatgttc
    27061 ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat tatctagagt
    27121 atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt ttttccattg
    27181 ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa agtgggttta
    27241 atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca caactagctt
    27301 acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt tctaagccgc
    27361 taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg gctgatatct
    27421 tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata
    27481 tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc ggcattggcg
    27541 gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca cgtttgaaac
    27601 cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca tttgaacatc
    27661 tgctatgtgg cgcaagcatg ttacagattg gtacagagct tcataaagaa gggccggaaa
    27721 ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga tatacgtcaa
    27781 ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa ggtcatgatt
    27841 gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat
    27901 tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata atatgaatga
    27961 tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag aactagttac
    28021 ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga
    28081 accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag ggccattggc
    28141 tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc tagctacttc
    28201 aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg tgcggcaagc
    28261 agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa
    28321 tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg aatgtacacc
    28381 gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc ctttagctgg
    28441 tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat tgattgggct
    28501 gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat ggcaaggagc
    28561 tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat tttatgggat
    28621 gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc aaacacatcc
    28681 aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact cacagattag
    28741 tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag aagggcatga
    28801 tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt attatcagat
    28861 gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc agcgagctgt
    28921 cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc agaagctctc
    28981 tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa
    29041 cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg cccctaaaat
    29101 cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca gtgattgttc
    29161 tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga agatcacacc
    29221 ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta ctcatttgac
    29281 aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag aagatttgct
    29341 atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa atccatggaa
    29401 atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct aataactagt
    29461 gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga taacagctgg
    29521 agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact gtttcccgca
    29581 tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc aaaagaggtg
    29641 gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg acaaatcact
    29701 tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac aaaagaaaga
    29761 gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat gatcgtacga
    29821 aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa agcagctggc
    29881 gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt agtcacatat
    29941 gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac agatgttatt
    30001 ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat ccctgtaaaa
    30061 atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa agaaggaaat
    30121 gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca
    30181 aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg
    30241 gctgagtcgg tgagtgagtg atgtactttc ccctaattga tttttccctc atgtggactt
    30301 cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc gtgtcgtttc
    30361 ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat tggctgcctc
    30421 tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca ctagtcctgc
    30481 tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct tcagtcatct
    30541 gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca ctagcaggcg
    30601 tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt ggggtattga
    30661 aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt aacgtagcta
    30721 tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa atatttcaaa
    30781 aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt ttagttgcac
    30841 tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta gaaaaacgtt
    30901 ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca ccatttctat
    30961 tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc
    31021 gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa tagaatcctc
    31081 gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca cgatgcctga
    31141 tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa gccgagaatg
    31201 gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca aactgtcatt
    31261 tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag taaaaactga
    31321 aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag aggagatgcc
    31381 tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga
    31441 agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa aagcaaagca
    31501 aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat tgaaaagacg
    31561 ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt tagaggaaat
    31621 agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc atgattatga
    31681 atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg agtgaaaaat
    31741 atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa agaagcactg
    31801 tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg tgattccgct
    31861 acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc tgtgatccaa
    31921 aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg gacagtcaaa
    31981 gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag tgaagtactg
    32041 gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga tacagaggtg
    32101 tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag tcgctttcgc
    32161 atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt
    32221 gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt tttccacgca
    32281 gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt cgataaaggg
    32341 ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg caatatacgc
    32401 gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt gaagtttctt
    32461 gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact cttgattact
    32521 tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg actgggagtg
    32581 ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct agatcatcca
    32641 aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat ccaatgatta
    32701 gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc ggcaaacaag
    32761 gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt tcattgcttc
    32821 gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga ttttgaacga
    32881 tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg gaacagctgc
    32941 attagtctca ggcgtggatc ccgtttacac aaaaatagat ggtagttgga aagaatcaac
    33001 tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact
    33061 agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata tccaagatat
    33121 ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt ctgctggatt
    33181 cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag catatcaaat
    33241 aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca ctactgaaaa
    33301 aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa aaaacaagaa
    33361 agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt acgaaatagc
    33421 tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac gatctctcgg
    33481 cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt attttatctg
    33541 cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata gctttcaaac
    33601 gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc tgttcagatg
    33661 aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta ataaagatag
    33721 gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct gtcaaaacgg
    33781 cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa ccatatttat
    33841 gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga ttgaatgtat
    33901 tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt gatcgattcg
    33961 tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa gtttttccag
    34021 aaccagaagg accgataaat acatagcaat aattcattgt catttctcct ttgatgattg
    34081 aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact ttgctttttt
    34141 gtattttata ccagcaagcc agtcacaata gcacttaaaa agctgactaa agtcgctgtt
    34201 agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt taatcccttc
    34261 atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag gaagacagat
    34321 ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc tgtcatagca
    34381 acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac tgcttctttc
    34441 catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa
    34501 gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc aacaaaaccg
    34561 ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta ttctcccaac
    34621 atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc tttttcttct
    34681 aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt caaaacaagg
    34741 gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat cgacatagaa
    34801 actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag gaagggtaat
    34861 tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc tactccatta
    34921 taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac agctttcata
    34981 aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat aaatacaatt
    35041 ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca
    35101 aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc caatacagaa
    35161 acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg
    35221 aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat caaaaaagca
    35281 agccctaatg tgacaaataa accaataata cctaataaat aactcaaata atcactccta
    35341 ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt tagtgaaatt
    35401 ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa gcgaacaaaa
    35461 taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa actatcgatt
    35521 aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt aatatagatt
    35581 ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca atgtttgttc
    35641 atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt cagctagttt
    35701 atcataagca acttgagcga ttggattcgt tactgcactg attgataaag cgatcaagac
    35761 ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca atccttgtat
    35821 tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg ctaaatgttt
    35881 tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac gcccggtaat
    35941 gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg taggactttg
    36001 cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt cttcaggacg
    36061 taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt cgatcaatcc
    36121 ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt tagaggcttc
    36181 ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca tgtcagtagg
    36241 agattgataa acagattctt taccagtaat tttttcaata atacgtttga ttacaggaaa
    36301 tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa agtggaagga
    36361 atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa tgttcagcgg
    36421 atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac cagctgcatg
    36481 tcctttttgg ctttcatgat acagttggtt caagcaagta gcgagctttc cacttcctgg
    36541 gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat tttttccaaa
    36601 accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt ctgagtgttt
    36661 atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg caggctgtcc
    36721 actatagcga gtgataacga cactgttgat taataagcca taaccacgta attcatcgat
    36781 taaccgcaag atatccatat cataagtaat cccataatca ccacggatct tattacgttc
    36841 aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt tttgcagcaa
    36901 tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac
    36961 taacttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt ccaaaatata
    37021 cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca ctaggttccc
    37081 tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa
    37141 ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt attcattggt
    37201 atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat
    37261 ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa gatcccgaat
    37321 tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt gatgagcatc
    37381 agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga taaaggtcat
    37441 cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa ggctgtacca
    37501 aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac aattttttac
    37561 agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga ggaggcaagt
    37621 agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga ggagacgaac
    37681 gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga aaaagcaagc
    37741 atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt ccgcgaagaa
    37801 gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt aaaaagaagc
    37861 tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga tcgtttttta
    37921 caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag ctattacaaa
    37981 atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct gccagaaatt
    38041 tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga gtcaacggac
    38101 tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac agaaattgaa
    38161 cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa cgacaataaa
    38221 ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa gaagaagcaa
    38281 gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga aacaatccag
    38341 cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc agccatcgta
    38401 gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt cggtcaagca
    38461 atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca atctaatgaa
    38521 ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg aagaacaaac
    38581 agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc ctctggatga
    38641 cacagaagaa atccctatgc tagaggaaac aaaaatacat gaaaaagaaa aagtaactcc
    38701 gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa atcccgggta
    38761 tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc
    38821 agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc cacaattatt
    38881 agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg gaaagacaag
    38941 cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg gtctggaacg
    39001 gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt atacgttgaa
    39061 tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac gataccaagt
    39121 ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg tccaagatga
    39181 ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg cagggcagat
    39241 cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac ttggttttac
    39301 cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc agagcgaatt
    39361 tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa atccatccta
    39421 cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag gacagtcttt
    39481 aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg gagactaccc
    39541 acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa tggtgagaaa
    39601 gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt ccaaaggact
    39661 tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa atggacgtga
    39721 ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa tagcacagct
    39781 tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta ttccgaattt
    39841 ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa aagatgcgtt
    39901 aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg gaccatgggt
    39961 catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg tctgcttgag
    40021 aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg cgggcatggt
    40081 ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc aaccttggat
    40141 ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg ttgtgttctt
    40201 tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa
    40261 aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca agttgcagta
    40321 caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa
    40381 caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtggtc ttcaaaaaag
    40441 gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat aacggcttca
    40501 tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg atgtttactg
    40561 tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat ggacaatcgg
    40621 tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg cgaatattgc
    40681 cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa
    40741 gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct atcagcaaat
    40801 ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg
    40861 ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat aaaggacaga
    40921 gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca atcgtcgcaa
    40981 tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa aagttgacgc
    41041 atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta tttggtttgg
    41101 taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa
    41161 aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg attattttta
    41221 cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca atattgactt
    41281 atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt ttcttgctga
    41341 tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt gaaagcgtga
    41401 cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc agtcggctga
    41461 tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca aaagcagata
    41521 tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa aaaaatatgc
    41581 gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa tatgacccaa
    41641 attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga aaaatataat
    41701 cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga gtccgctgtt
    41761 tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc
    41821 aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga ttagcttgcg
    41881 gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta tcgattctgg
    41941 tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa gcatcatttg
    42001 caacgacatt attgatggtt ttctttttag aattacgcta tacattatta ggagcaagtc
    42061 ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct caatcactga
    42121 atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc tggaataaaa
    42181 gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc aatatgatcg
    42241 gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt gctttgacgg
    42301 ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc tttacaggtt
    42361 tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact ttcggattga
    42421 ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca ttcaaaaaag
    42481 aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc
    42541 cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt tgcttgtctg
    42601 ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca tcgaaaagtg
    42661 ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc ggcgttagct
    42721 tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat caaaatagct
    42781 gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg
    42841 acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt ttattaaaaa
    42901 cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg caacagtcaa
    42961 ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta tgatgcaagc
    43021 tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt gctataatag
    43081 ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa aaaattgatg
    43141 caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg ggtagtctat
    43201 ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc tacgacattg
    43261 tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt cggatttcca
    43321 aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc tgcttgtcgg
    43381 ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc tgctgcactg
    43441 atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt taataatggg
    43501 atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct tagtgcaatg
    43561 agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt gattatcgaa
    43621 caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt attgactgac
    43681 ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg tgggttagct
    43741 gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact taaagatttt
    43801 ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc tatcattcgg
    43861 gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat gatcgaccaa
    43921 gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca gcaactggaa
    43981 ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct gatctccatg
    44041 gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa aaaagatcgg
    44101 atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat tcttcttcct
    44161 tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt tggctatgat
    44221 cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt ctctttattc
    44281 acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat ccgattaact
    44341 gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt agttggcgga
    44401 tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg cggctatcac
    44461 tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt tttcttaaaa
    44521 actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt tttgctgaat
    44581 cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc tgtaggaagt
    44641 attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa
    44701 aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt tgctgtcact
    44761 tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa ttttagtttc
    44821 atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg aaacgaggaa
    44881 attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc ggaacgtatc
    44941 aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg gaaaatttat
    45001 aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa cagtaaagat
    45061 tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa
    45121 gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc tggctttgtc
    45181 aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc ttatgactct
    45241 aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt aaatgatgaa
    45301 ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa tacctatgct
    45361 tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac aaatggtgca
    45421 gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga agggaaaatt
    45481 gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga agaaattatt
    45541 ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca tgcaaataat
    45601 ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc
    45661 gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc catcggcatt
    45721 gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaata
//
LOCUS       DAZKMK010000010        45670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000010 DAZKMK010000000
VERSION     DAZKMK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.010"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG8V1_000610"
     CDS             6..245
                     /locus_tag="ACG8V1_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328728.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG8V1_000611"
     CDS             312..521
                     /locus_tag="ACG8V1_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328729.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG8V1_000612"
     CDS             581..1252
                     /locus_tag="ACG8V1_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0328730.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG8V1_000613"
     CDS             1305..3281
                     /locus_tag="ACG8V1_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0328731.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACG8V1_000614"
     CDS             3294..4049
                     /locus_tag="ACG8V1_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0328732.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACG8V1_000615"
     CDS             4046..4870
                     /locus_tag="ACG8V1_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0328733.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACG8V1_000616"
     CDS             4880..5128
                     /locus_tag="ACG8V1_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328734.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACG8V1_000617"
     CDS             5171..7270
                     /locus_tag="ACG8V1_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0328735.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACG8V1_000618"
     CDS             complement(7308..9365)
                     /locus_tag="ACG8V1_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0328736.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACG8V1_000619"
     CDS             complement(9479..9691)
                     /locus_tag="ACG8V1_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328737.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACG8V1_000620"
     CDS             9878..10345
                     /locus_tag="ACG8V1_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328738.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACG8V1_000621"
     CDS             10342..10584
                     /locus_tag="ACG8V1_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0328739.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACG8V1_000622"
     CDS             10602..10904
                     /locus_tag="ACG8V1_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328740.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACG8V1_000623"
     CDS             10976..13030
                     /locus_tag="ACG8V1_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328741.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACG8V1_000624"
     CDS             13030..15642
                     /locus_tag="ACG8V1_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0328742.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACG8V1_000625"
     CDS             15639..18170
                     /locus_tag="ACG8V1_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0328743.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACG8V1_000626"
     CDS             18222..18554
                     /locus_tag="ACG8V1_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328744.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="ACG8V1_000627"
     CDS             18555..19148
                     /locus_tag="ACG8V1_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328745.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="ACG8V1_000628"
     CDS             19311..21005
                     /locus_tag="ACG8V1_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0328746.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8V1_000629"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8V1_000629"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0328747.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="ACG8V1_000630"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="ACG8V1_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="ACG8V1_000631"
     CDS             24078..25283
                     /locus_tag="ACG8V1_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0328748.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="ACG8V1_000632"
     CDS             25299..25943
                     /locus_tag="ACG8V1_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328749.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="ACG8V1_000633"
     CDS             25954..26760
                     /locus_tag="ACG8V1_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328750.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27012
                     /locus_tag="ACG8V1_000634"
     CDS             26782..27012
                     /locus_tag="ACG8V1_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328751.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27558..27833
                     /locus_tag="ACG8V1_000635"
     CDS             27558..27833
                     /locus_tag="ACG8V1_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328752.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27875..28297
                     /locus_tag="ACG8V1_000636"
     CDS             27875..28297
                     /locus_tag="ACG8V1_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328753.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG8V1_000637"
     CDS             28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG8V1_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0328754.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29920..30255
                     /locus_tag="ACG8V1_000638"
     CDS             29920..30255
                     /locus_tag="ACG8V1_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328755.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30402..30596
                     /locus_tag="ACG8V1_000639"
     CDS             30402..30596
                     /locus_tag="ACG8V1_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328756.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30628..33000
                     /locus_tag="ACG8V1_000640"
     CDS             30628..33000
                     /locus_tag="ACG8V1_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0328757.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33061..34521
                     /locus_tag="ACG8V1_000641"
     CDS             33061..34521
                     /locus_tag="ACG8V1_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0328758.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34532..36736
                     /gene="topB"
                     /locus_tag="ACG8V1_000642"
     CDS             34532..36736
                     /gene="topB"
                     /locus_tag="ACG8V1_000642"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0328759.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36752..36901
                     /locus_tag="ACG8V1_000643"
     CDS             36752..36901
                     /locus_tag="ACG8V1_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328760.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36975..37568
                     /locus_tag="ACG8V1_000644"
     CDS             36975..37568
                     /locus_tag="ACG8V1_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0328761.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37584..38483
                     /locus_tag="ACG8V1_000645"
     CDS             37584..38483
                     /locus_tag="ACG8V1_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0328762.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38486..38971
                     /gene="ssb"
                     /locus_tag="ACG8V1_000646"
     CDS             38486..38971
                     /gene="ssb"
                     /locus_tag="ACG8V1_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0328763.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39337..39597
                     /locus_tag="ACG8V1_000647"
     CDS             39337..39597
                     /locus_tag="ACG8V1_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328764.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40038..40244
                     /locus_tag="ACG8V1_000648"
     CDS             40038..40244
                     /locus_tag="ACG8V1_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0328765.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40244..40495
                     /locus_tag="ACG8V1_000649"
     CDS             40244..40495
                     /locus_tag="ACG8V1_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328766.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40510..40947
                     /locus_tag="ACG8V1_000650"
     CDS             40510..40947
                     /locus_tag="ACG8V1_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328767.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40940..41647
                     /locus_tag="ACG8V1_000651"
     CDS             40940..41647
                     /locus_tag="ACG8V1_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328768.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            41812..42012
                     /locus_tag="ACG8V1_000652"
     CDS             41812..42012
                     /locus_tag="ACG8V1_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328769.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            42028..42207
                     /locus_tag="ACG8V1_000653"
     CDS             42028..42207
                     /locus_tag="ACG8V1_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328770.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42548..42811
                     /locus_tag="ACG8V1_000654"
     CDS             42548..42811
                     /locus_tag="ACG8V1_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0328771.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42805..43155
                     /locus_tag="ACG8V1_000655"
     CDS             42805..43155
                     /locus_tag="ACG8V1_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0328772.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43179..43793)
                     /locus_tag="ACG8V1_000656"
     CDS             complement(43179..43793)
                     /locus_tag="ACG8V1_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0328773.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44107..44529
                     /locus_tag="ACG8V1_000657"
     CDS             44107..44529
                     /locus_tag="ACG8V1_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328774.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44539..44742
                     /locus_tag="ACG8V1_000658"
     CDS             44539..44742
                     /locus_tag="ACG8V1_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328775.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44953..45537
                     /locus_tag="ACG8V1_000659"
     CDS             44953..45537
                     /locus_tag="ACG8V1_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0328776.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16743 a         7205 c         9261 g        12461 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag
    24721 gttgtatgtc aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac
    24781 attggggtga aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc
    24841 ctggcagcag tttagataaa agttccttca gcggtggtca attatttgga acgaatccag
    24901 gcggtgaatt ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat tttttatatt cacgaagata aggtcaaaga tcacgtgaaa
    26461 gacgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
    26881 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat
    26941 accttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
    27001 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
    27061 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
    27121 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
    27181 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
    27241 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
    27301 acaaataacc ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg
    27361 tgttaggtag gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga
    27421 tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt
    27481 gtgtagcaaa atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa
    27541 taaatttgga ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata
    27601 aaattgacaa acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc
    27661 aatacctaaa agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag
    27721 accaatttga tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt
    27781 tagaagaata tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat
    27841 agtattgtga atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt
    27901 atatctttac gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat
    27961 atctaattat agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga
    28021 aaaaataaat ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac
    28081 cgaaatggtt tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac
    28141 aaattcaact gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca
    28201 aaccaaaaaa tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt
    28261 agatccattt gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa
    28321 taaatcgcct gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc
    28381 aacatacatt gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc
    28441 cgaagaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
    28501 aggagaaact ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc
    28561 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
    28621 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
    28681 ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca
    28741 taaaaaagct tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg
    28801 aagtgatctg aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat
    28861 ttatcaagat gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc
    28921 caaaacagat accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac
    28981 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
    29041 cactaagcat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta
    29101 tactgagatt gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac
    29161 attagacgca atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga
    29221 tcgaatgatc ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac
    29281 cttaacaaaa gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa
    29341 tgaagtatat aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg
    29401 aaccaagctt tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac
    29461 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa
    29521 aagcctttat ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga
    29581 tttaaaaaag cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat
    29641 aaaaccatta atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg
    29701 aacactcaaa cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg
    29761 taggaaacaa ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac
    29821 aattaatgca catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag
    29881 aatccaaatg gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa
    29941 tggaaagtca tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta
    30001 gaaaaagagc atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat
    30061 caatttttag ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt
    30121 gaaaaatatt ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg
    30181 gaaataaatg aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct
    30241 ctcacggaat gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact
    30301 ctttttcaat gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat
    30361 caaaagaaag tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg
    30421 gtgttgtggt tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta
    30481 gagcttttgt agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt
    30541 taaatctgac tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa
    30601 acgatatttt acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt
    30661 cggttgggaa agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt
    30721 caaaagctat ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat
    30781 ggtcgttcat ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa
    30841 gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa
    30901 atgggctata acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa
    30961 gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa
    31021 tatcaacgga agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa
    31081 aatatcctcc ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa
    31141 aatccaacca tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac
    31201 gggggatttg aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt
    31261 cgtgaattac ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat
    31321 ttctatcgag tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg
    31381 gagatcgtaa cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca
    31441 gaagcaggtt attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag
    31501 atccgaatgg atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca
    31561 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
    31621 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
    31681 acggcagaag agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
    31741 aaaaagcata cccattcttc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
    31801 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
    31861 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
    31921 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
    31981 cgagaaacag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
    32041 atcaaaaaac cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
    32101 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
    32161 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
    32221 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
    32281 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
    32341 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
    32401 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
    32461 tttggaatgg acacttcgga gaaagctatt ccttatattg cagcatggac gaagaatggt
    32521 cagacgttgg atgataagga agcggcggaa cgtgggaaaa tcatgaatga tgtcagccgt
    32581 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
    32641 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagag
    32701 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
    32761 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
    32821 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
    32881 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
    32941 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
    33001 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
    33061 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
    33121 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
    33181 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
    33241 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
    33301 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
    33361 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
    33421 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
    33481 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
    33541 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
    33601 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
    33661 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
    33721 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
    33781 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
    33841 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
    33901 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
    33961 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
    34021 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
    34081 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
    34141 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
    34201 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
    34261 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
    34321 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
    34381 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
    34441 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
    34501 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
    34561 gtcacaagcg aaagcttatg ccgatagctt ttctaaagct acccgaaaag atgggtattt
    34621 tgagatccaa gatcgattgt ttacaggaga aactgtgatc acgtatggtt ttggtcattt
    34681 agtcgagtta gattcgcccg atatgtacga tgaaaaatgg aaacaatggg cattagaaca
    34741 tttgcctatt ttccccaatc aataccacta tcatgttcct aaagataaga aaaagcagtt
    34801 tagtgtcgtc aaacgacagc tgcagtcagc tgatacgatt gtgatcgcga ctgatagcga
    34861 tcgtgaaggg gagctgattg cctggtcaat cattcaacaa gcaggcgcca atcaaggaaa
    34921 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
    34981 acagttacga gacgcagaag agacctatcc aaaatttgaa gaagtacaag cacgtcagat
    35041 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
    35101 aggcatacca ggaagttttt ctttaggtag agtccaaacc ccaacactct atatgattta
    35161 tcaattgcag gaaaagatta gaaacttcca aaaagaacca tattttgaag gaaaagccca
    35221 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccaaatgaaa cccaagcgac
    35281 acaagaagca tttgaagcct atttaaaaga aaaaggtgtt caattaggaa aacagccagg
    35341 tacgatcctt caagtcgaaa cagagaaaaa aagtgcggct agcccacgtc ttttttcttt
    35401 atcgagcctt cagtctaaaa tgaatcaact catgaaagct agtgccaaag atactttgga
    35461 agccatgcaa gggttgtatg aaggaaaata tctaagctat ccgcgaacgg atacccctta
    35521 tattacggaa ggggaatatg cgtatctgtt ggatcactta gacgaataca agcatttttt
    35581 aaaagccgaa gcgattccga ctcctattca tacaccgaat agtcggtatg tgaataataa
    35641 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
    35701 tgaacaacta agtccactgc agcaggctat ttatgaacaa gtgttaaaaa caacggtggc
    35761 catgtttgcg gaaaagtata cctatgaaga aacaacgatc ttgacgcaag ttcagcagct
    35821 tcagttaaag gcaatcgaaa aggtgccgct agatctaggt tggaagaagt tatttggaaa
    35881 agagagcgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
    35941 aatggtgacg gtggatctac aagttcttga aaaagaaacc aagccaccac agccttatac
    36001 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
    36061 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
    36121 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
    36181 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
    36241 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
    36301 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
    36361 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
    36421 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
    36481 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
    36541 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
    36601 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
    36661 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
    36721 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
    36781 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
    36841 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
    36901 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
    36961 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
    37021 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
    37081 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
    37141 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
    37201 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
    37261 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
    37321 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
    37381 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
    37441 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
    37501 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    37561 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
    37621 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
    37681 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    37741 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    37801 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
    37861 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
    37921 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
    37981 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
    38041 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
    38101 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
    38161 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
    38221 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
    38281 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
    38341 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
    38401 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
    38461 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
    38521 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
    38581 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
    38641 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
    38701 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacaac gtgtgtatgt
    38761 gacagaagtt gtggccgata acttccaact attagaaagt aaagcagcga cagaaagccg
    38821 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    38881 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    38941 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    39001 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    39061 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    39121 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    39181 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    39241 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    39301 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    39361 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    39421 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    39481 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    39541 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    39601 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    39661 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    39721 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    39781 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
    39841 cgttatctct ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg
    39901 acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt
    39961 attctggcca acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag
    40021 aggaggcaga atcaaacatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat
    40081 tattcccaaa ctatcatgca ccacatactg ttgaatgcga ttgtggagaa agagcgaaat
    40141 gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt
    40201 atggcgatta cattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa
    40261 attattgact gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa
    40321 agtagcagaa gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga
    40381 tgcaccgact atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca
    40441 agaaaacatg gaaacttatg aaatcgaata ttggttaacc aaattagaga actaaaaagg
    40501 agaaaaaata tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg
    40561 gcacaaaaaa tggaggatat gacgttccta taccatgaaa cacgtgtacc caaaaaacat
    40621 tacaaggaaa aattgtcagt cgcagtggaa gaaatgattg aatcaggtgt cgaaatgaat
    40681 ctcattgcga cgtactatcg aacactggaa gaactgaaaa aacaaaatgg aaaatggttt
    40741 ttccaagcct tactatgctt ggaagtaggt gtcaaaccaa gcactatcaa accttccgaa
    40801 tatcaagcct tagaactgac ttatgcaaaa ttcgcacaga ttaagaaagc taaagcaatg
    40861 gctagcgact ggctagatac ttttgaaagt atcgaaaaaa atggtgcatt ttatacgatt
    40921 gaatcggagg agaaagataa tgaataaggt ggtttatcag ctaacaacag aagatgtttc
    40981 gcaattttta acaagcaaag aacacttgaa tgaagtaatc agacggattc aaaaagactt
    41041 tagttgtatg gaagaaatga agtactttat tcaaagagtg gaagaacagt tgtatatgga
    41101 aagcgaaaca agtcaaacga aggtcacagt cagccataat catttagacg tgtataaacg
    41161 tcgtcctgaa acggtaaaaa aaggaacaga gcttctacga ctagaaacag aaaaatacat
    41221 catgaatgtg tacctagaaa ggagtgagct ggtcgaaaac tttgtcagct acacgatttg
    41281 taggaaacac tattactcag aatcagaata ttattcaatt ttcgagagca tggaattaat
    41341 ggaagaagaa atggaagagc taaacagcaa aaatgcgacg gtagacgatt attaccttgt
    41401 atttactagg tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    41461 actcgctgta cccgaagcat ttttgaggga atatgtagaa gaagtagata acccaggcga
    41521 cgactggtac acgactttaa ctgatttttt agacacctac acttacgacg atacagaaga
    41581 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    41641 taaataacca tttataccga aaagtgagga tgtgtgaaat aagaacaaaa aaccttgaaa
    41701 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    41761 gcgaaataag cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa
    41821 atcattcctc tattgttcgt actgtttgtt ggcgagatac ttatgcgtca tgctgcagct
    41881 gtacatttaa acttcgtagt attcttagga atactggtat tttgtttgat cttttggcga
    41941 tttgtattag cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg
    42001 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
    42061 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa
    42121 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
    42181 tttgatatac cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
    42241 gcacttaatt gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat
    42301 caataactat gaaatcttta aagtatccag aagagattat ttttagatca atttttttct
    42361 tacctgattc taaagatttt gttttcttaa acatattatg atttcctttc gactattttc
    42421 atataaatgt taacatagtt tatggattca tggaatgtta atctaattta atagaaaagg
    42481 ataattaagt atatacgttt tttcttatgt atgttatact atgtatatac agaaagaggt
    42541 gtctactatg gaaatgttac ctataaaaaa atggggaaat agtaatggcc ttagattgcc
    42601 aaagtatatt atggaatatc ttggaattca tacagaagac aaagtgaaga taatacaaga
    42661 agaagtaaac ggtcagaagc ggctaattat cgaagcggct aatatagaaa atgaattaac
    42721 aattgaacag ttatttgaaa attacactga tgaaagaaat catgtgacga ttcaaaattt
    42781 aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta gacaaggcga tattctatta
    42841 ttgaatacag cacctcgatc tggacacgaa cagacaggta aaagacctta tattgtttta
    42901 agtcatgata ttattgcgga ttatagtaac gttgttacag ttgctccaat ttctagtaca
    42961 actagaaact atccgctata tgtaaatata aactcagaat atgaaatgaa gacaacagga
    43021 aaagtattat tagatcaact aacgacaatt gattatgaag ccagagagtg tatttttttg
    43081 gaaaaagcgc atgatagttt agttgaggag ttattgatga aagttagaac agtttttcaa
    43141 aaagtaagca aataaaatag aaacagcagc atgatcaact attcgtgatt gaccatgctg
    43201 tcttttttta ttctatatac ggtttgtctc gtaacattgt agtcttttgc aatcttagcg
    43261 atcgcaacac ctttttttag atcctcaacg atgtttttat aaacaagacg acgttgggga
    43321 tcttttgcat tggcactgta tagcttagga cgtcctttat aaacaccgtt cgcttttgca
    43381 atcttaatgc cttgtgcctg acgtcgttta gattcggtac gttcttgttc agcgatcatg
    43441 gccaaaattt gaatgatcaa atctttaatg aatttatcca ggagaggatt gccaattgct
    43501 tcggccatga tcggtagact tgtgatgatc agctgcacat ttttcttctt caagtaattg
    43561 actgaagcga tgatctcgtc gtaatttctt ccaagtcgat caatagcttc cacaataaaa
    43621 atatcttgat ctcggacgaa atccagtgct tcttgaaaaa caagtcgatg agtaatcgtt
    43681 gcgccagatt gtttctcaat gaaaattctc tctgcaccga attttttcat ttcttcaatc
    43741 tgccgttgtt cattttgatc gatggatgag acacgtatgt atgcaactct caaaattttt
    43801 acctactttc tgcaactatt taaaaaacac cctaatcata cacgagaaaa ccagattttt
    43861 caatgtcgcg gtcgtgtcta gttagggtat accttatata gacaaaaact aatgatggag
    43921 gaaatagtat gtgtcacttt tctttttaaa tggcaatatt ttttgtaaat aaatgtcaat
    43981 caagaaaatt taaacgatta tgacattaaa aatatatttt atgaattttc tagtatataa
    44041 cttgatttat ctgttaagat ttattaagaa aacgttttct tatttttgat ttcgaggtga
    44101 tagacaatga aaaataagtt tattttatct ttaggtattt taactattgg aattagtgta
    44161 actggaacta caattcctac atttgctgat agtacaaatg aaccaactac tgtagtagat
    44221 aataaccaag aattaactcc tttagatagg aacggaatag ccattggtaa cccaactaac
    44281 gacttaactg ttactgaaaa ttatttaata gtttctccat tacttgctcg agcagcttct
    44341 aaaacgatac gagtgacagg aacgatgatt tttgataaag ttggttggcc ttctagacct
    44401 cctcaatcac ggaattggaa agaaaaaaga aatggttatt ggtactatgg aaaattgtat
    44461 ttacaaagtt ataaatcagc tggaaacaat tggattgcga cttattcagg aaccttaact
    44521 agaaagtagg gattttttat gccagaattt gtgaacacac caaattttta tcaagatagc
    44581 gatattctaa gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt tcagcgaact
    44641 ttacaggaga cacaaaaaat tcaaaaatca atttctaatc tagaagattc tgaagaaaaa
    44701 agaaaaaaaa gagaccaaaa aacatatcaa aaagaaaaat aatgatttta aaaaaagagt
    44761 gtctagtcga cattcttttt ttctaattga aagtgttcat aaaatcaccc atttctggac
    44821 actttctttt tttgctttat ttttcaaaaa aacaattcat ttcatgttca taaacccata
    44881 acaatatcta tgttcagtta aactatatgg ataaagttat gttacaataa aattaatgaa
    44941 ggagtgattt ttttgaaaat aggatacgcc cgggtatcta ctggtttaca aaatttggat
    45001 ttacaagaag acagcctaaa taaatttggt tgtgaaaaaa tttttactga tcatatgagt
    45061 ggggcaaaaa gtaatcgacc aggattagaa atggccattg attttgttcg ctctggcgat
    45121 acacttgtcg tttggcgctt agatcgttta ggtagaaata tggaagattt gatttcaatc
    45181 gtcaatcgac tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa tattacgatg
    45241 gacaaatcaa gttctacagg acagctcatg tttcatttgt ttgcggcatt tgcagaattt
    45301 gagcgtaatt tgatcctgga acgatccgca gcaggacgag cagctgctcg tgcaagagga
    45361 cggtttggtg gtcgtcctga aaaattgtct gaacaagatt tggaattact gaagacttta
    45421 gtggatagcg gtacacctat taagactatt gcggaacggt ggaacgtctc aagaaccacc
    45481 atttatcgtt atttggcaaa aatggaggag aaaaatggaa agacaaattc tcattagttt
    45541 ttgtcattta atgctgaaaa tttttgccat ttatctagaa aagtgacaat agtatgagaa
    45601 ttttttgtaa gtgtctactg ataacttatt ctcaaatgtt gtaaggattt tattcatttg
    45661 ttaatattaa
//
LOCUS       DAZKMK010000011        44807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000011 DAZKMK010000000
VERSION     DAZKMK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.011"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..640
                     /locus_tag="ACG8V1_000660"
     CDS             8..640
                     /locus_tag="ACG8V1_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328777.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(774..899)
                     /locus_tag="ACG8V1_000661"
     CDS             complement(774..899)
                     /locus_tag="ACG8V1_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0328778.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1033..1926)
                     /locus_tag="ACG8V1_000662"
     CDS             complement(1033..1926)
                     /locus_tag="ACG8V1_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0328779.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2108..3034)
                     /locus_tag="ACG8V1_000663"
     CDS             complement(2108..3034)
                     /locus_tag="ACG8V1_000663"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0328780.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3119..3880)
                     /gene="pflA"
                     /locus_tag="ACG8V1_000664"
     CDS             complement(3119..3880)
                     /gene="pflA"
                     /locus_tag="ACG8V1_000664"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0328781.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4124..6355)
                     /gene="pflB"
                     /locus_tag="ACG8V1_000665"
     CDS             complement(4124..6355)
                     /gene="pflB"
                     /locus_tag="ACG8V1_000665"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0328782.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6632..9082)
                     /gene="parC"
                     /locus_tag="ACG8V1_000666"
     CDS             complement(6632..9082)
                     /gene="parC"
                     /locus_tag="ACG8V1_000666"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0328783.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9094..11139)
                     /gene="parE"
                     /locus_tag="ACG8V1_000667"
     CDS             complement(9094..11139)
                     /gene="parE"
                     /locus_tag="ACG8V1_000667"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0328784.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11305..11739)
                     /locus_tag="ACG8V1_000668"
     CDS             complement(11305..11739)
                     /locus_tag="ACG8V1_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0328785.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11866..12504
                     /gene="plsY"
                     /locus_tag="ACG8V1_000669"
     CDS             11866..12504
                     /gene="plsY"
                     /locus_tag="ACG8V1_000669"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0328786.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12636..13511)
                     /locus_tag="ACG8V1_000670"
     CDS             complement(12636..13511)
                     /locus_tag="ACG8V1_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0328787.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13593..14384)
                     /gene="codY"
                     /locus_tag="ACG8V1_000671"
     CDS             complement(13593..14384)
                     /gene="codY"
                     /locus_tag="ACG8V1_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0328788.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14402..15802)
                     /gene="hslU"
                     /locus_tag="ACG8V1_000672"
     CDS             complement(14402..15802)
                     /gene="hslU"
                     /locus_tag="ACG8V1_000672"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0328789.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15816..16364)
                     /gene="hslV"
                     /locus_tag="ACG8V1_000673"
     CDS             complement(15816..16364)
                     /gene="hslV"
                     /locus_tag="ACG8V1_000673"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0328790.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16401..17306)
                     /gene="xerC"
                     /locus_tag="ACG8V1_000674"
     CDS             complement(16401..17306)
                     /gene="xerC"
                     /locus_tag="ACG8V1_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0328791.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17504..18880)
                     /gene="trmFO"
                     /locus_tag="ACG8V1_000675"
     CDS             complement(17504..18880)
                     /gene="trmFO"
                     /locus_tag="ACG8V1_000675"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0328792.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18849..20927)
                     /gene="topA"
                     /locus_tag="ACG8V1_000676"
     CDS             complement(18849..20927)
                     /gene="topA"
                     /locus_tag="ACG8V1_000676"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0328793.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21049..21906)
                     /gene="dprA"
                     /locus_tag="ACG8V1_000677"
     CDS             complement(21049..21906)
                     /gene="dprA"
                     /locus_tag="ACG8V1_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0328794.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21962..22729)
                     /locus_tag="ACG8V1_000678"
     CDS             complement(21962..22729)
                     /locus_tag="ACG8V1_000678"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0328795.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22722..23582)
                     /gene="ylqF"
                     /locus_tag="ACG8V1_000679"
     CDS             complement(22722..23582)
                     /gene="ylqF"
                     /locus_tag="ACG8V1_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0328796.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23842..24240
                     /locus_tag="ACG8V1_000680"
     CDS             23842..24240
                     /locus_tag="ACG8V1_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0328797.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24477..25019
                     /locus_tag="ACG8V1_000681"
     CDS             24477..25019
                     /locus_tag="ACG8V1_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0328798.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25102..25653)
                     /gene="lepB"
                     /locus_tag="ACG8V1_000682"
     CDS             complement(25102..25653)
                     /gene="lepB"
                     /locus_tag="ACG8V1_000682"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0328799.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25805..26032)
                     /locus_tag="ACG8V1_000683"
     CDS             complement(25805..26032)
                     /locus_tag="ACG8V1_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0328800.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26029..26547)
                     /gene="msrA"
                     /locus_tag="ACG8V1_000684"
     CDS             complement(26029..26547)
                     /gene="msrA"
                     /locus_tag="ACG8V1_000684"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0328801.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26567..27280)
                     /locus_tag="ACG8V1_000685"
     CDS             complement(26567..27280)
                     /locus_tag="ACG8V1_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0328802.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27283..28179)
                     /locus_tag="ACG8V1_000686"
     CDS             complement(27283..28179)
                     /locus_tag="ACG8V1_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0328803.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28337..29179)
                     /locus_tag="ACG8V1_000687"
     CDS             complement(28337..29179)
                     /locus_tag="ACG8V1_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0328804.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29311..29964
                     /locus_tag="ACG8V1_000688"
     CDS             29311..29964
                     /locus_tag="ACG8V1_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0328805.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30027..30554)
                     /locus_tag="ACG8V1_000689"
     CDS             complement(30027..30554)
                     /locus_tag="ACG8V1_000689"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0328806.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30574..31521)
                     /locus_tag="ACG8V1_000690"
     CDS             complement(30574..31521)
                     /locus_tag="ACG8V1_000690"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0328807.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31533..33416)
                     /locus_tag="ACG8V1_000691"
     CDS             complement(31533..33416)
                     /locus_tag="ACG8V1_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0328808.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33579..35657)
                     /locus_tag="ACG8V1_000692"
     CDS             complement(33579..35657)
                     /locus_tag="ACG8V1_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0328809.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35670..35945)
                     /locus_tag="ACG8V1_000693"
     CDS             complement(35670..35945)
                     /locus_tag="ACG8V1_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328810.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36307..36762)
                     /locus_tag="ACG8V1_000694"
     CDS             complement(36307..36762)
                     /locus_tag="ACG8V1_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328811.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36916..38124)
                     /locus_tag="ACG8V1_000695"
     CDS             complement(36916..38124)
                     /locus_tag="ACG8V1_000695"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0328812.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38236..38568)
                     /locus_tag="ACG8V1_000696"
     CDS             complement(38236..38568)
                     /locus_tag="ACG8V1_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0328813.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38716..39591
                     /locus_tag="ACG8V1_000697"
     CDS             38716..39591
                     /locus_tag="ACG8V1_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0328814.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39673..40254)
                     /locus_tag="ACG8V1_000698"
     CDS             complement(39673..40254)
                     /locus_tag="ACG8V1_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0328815.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40260..41519)
                     /locus_tag="ACG8V1_000699"
     CDS             complement(40260..41519)
                     /locus_tag="ACG8V1_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0328816.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41521..42567)
                     /locus_tag="ACG8V1_000700"
     CDS             complement(41521..42567)
                     /locus_tag="ACG8V1_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328817.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42802..43077)
                     /locus_tag="ACG8V1_000701"
     CDS             complement(42802..43077)
                     /locus_tag="ACG8V1_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0328818.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43320..44630)
                     /gene="der"
                     /locus_tag="ACG8V1_000702"
     CDS             complement(43320..44630)
                     /gene="der"
                     /locus_tag="ACG8V1_000702"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0328819.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT        12108 a         9419 c         7661 g        15619 t
ORIGIN      
        1 agatattatg gaaattcaac gtagaaccac taatttttta catttaggct ctttttttgc
       61 aactttgacg atcttatttt ttctatgtta ttcatacctt aagacctcac atgcgccttc
      121 tagtgcatta ggctggttgc ttttggatca ttctgatatc acagaagccg aagaaaatcc
      181 gttttttttg attttgtctt ctctttgcgg cttactgttt tctgtttatt tcggtgccta
      241 tttttttcat cgacatcctg gtacgttaaa cgaagcttca agcgtattga ttgcatttaa
      301 aacgttttct tttttagcaa ttctttttta ttcgtttaat cacaatacgc ttttcttctt
      361 tatcatgaat ggtttagtcg tgattttatc catttttact tattttattt tttcactcat
      421 ttttagccaa ttgaaatgtc cgctcttctt tcgattagtt cctgtcacct gttttattta
      481 cagcttgttt tccttttttg tccttgcttt aatgccactg accaaccctt cgacgatcca
      541 agtcgcagga aatctgttgg atatgctctt catcgtcagt ctaggtgtat ttatgtggca
      601 aagaaaaaac aaacaaaata aaaagcattt tgaagcctga ctattacgac ttcataaatg
      661 cttttttagt tttaaacact tcaatctaaa agaataaccc aagattggat aaattattaa
      721 tgactcgcca ttcgtttctt agtgattctg gattatcaaa tggacttcct aatctaatca
      781 gctagttctt cccataaaat gccatacttg actgaatcat aatattgatt atttaaaaat
      841 ctaacttttc ttatcactac ttcctttgtc attccacatt tctcaccatt tttttgcatt
      901 cctttgtttc cagaccatgt agtgaatcta ataggtggta gataggcaaa atactcaaat
      961 aattctttta accataaagg aagagaaagg ctcctcctgc aagagtcagt ataatttttg
     1021 cttactgctc tcttaggttt tactcactaa aactgttcca tattctttga tataagaaaa
     1081 tgcatgcggt ataaatttct tgctcagctc agaaaagatt tgtcgtttaa gaaaggctat
     1141 ttcctccatt gtcaatgaag gatccaatgt tggtaattca atctcaaaat tgtttttttc
     1201 gacaataggt aattctaatg tgaatgattc ttcttccttt atagtattcg tcacaagtag
     1261 agactgatgg aaaatactag gccggcgatt tttatattta ttcttacgta tcctgtcaac
     1321 gataacttct atttgatcaa aaaagtacga ttcatttacg tctccaataa gttcggctaa
     1381 ttctttgaat gccttcgtta caggaagctg attcttctgt acaaaaaagt aattagccaa
     1441 agtaagcggg aagtgttcta tagaggaaag tgtgataaaa cggtacttct catttcctgc
     1501 agttgtcctc agcaattctg tctctcggct gtttcttact ccataaaaat acgcttcttt
     1561 ccgttcatct tcgaaacaat acgcccaaat aacttctccc atcgctaatt tttgttcatc
     1621 aacagagagt ctcttcaatt gttcagctat ggtttcagca gccgaagtgg cttgttgcaa
     1681 gtaatcttta ggatcaaata ttggaaagtt caaatgatat cttcgttctt ccctcttgat
     1741 taatcctgca tcgatcaata aatcaagaaa atgggaaaat ttattttgcg aaaattcttt
     1801 tttcaattct cttagaatcg tttcttgttc tcgatgttca tatagataat cggtcaatga
     1861 agaaaaaagc ggatcatcgg tcagtttttg ataaaagttt tccttatttt ttgaataata
     1921 gttcatatat gttctctttt ctatccgttt tattttactt aaaaggcttt tacgtaaaaa
     1981 ggaggaagaa caagagtcat aattgactgt tattctcctc catttcttcg gcctttagta
     2041 tgttctatgt ttctttttat atcaatcgct tatccattca ttttgaatgc ttcatactac
     2101 catttgatta gctaaataca gccgtcaatt gaggtacgac ttgtttttta cgtgaaacga
     2161 cacctttaag caaagcacgg ttgttggtca aagttgtttg gaaagcttct tctacttttt
     2221 cttttccttt accagcaaca agtaattctg aatcactgtc taagatgttt gtgacaacta
     2281 atacgaacag atcgtagcca tttgctttca tttcatcttc cattgctgct tctagctctg
     2341 cttgtcgatc catcacttca ttaaggtcca ctgtattgac ttgagcgata cgaacgttgg
     2401 aactgcccat tgggaaactt tttgcatcca aatcaagaag aaccgcagct gatttatcac
     2461 ttagatttgt tcctgctttc aacatttcaa gtccataagt gtttagatcg acttcagcga
     2521 ttgctgcaag ttcttttgca gctgcttcat cttcaggcgt acatgttggt gatttgaaca
     2581 acaatgtatc tgaaatgata gctgagagca tgattcctgc gatttcttta ggaattgtta
     2641 cttggttttc tttatatagt tttaaaataa tcgtacttgt acatccaact ggttctgcac
     2701 gatagtacaa tggattagct gtttcaaagt ttgcaatacg atggtggtct actacagcta
     2761 aaatatttac ttcgctgata tctgaaatac tttgttggaa ttcattatga tccacaagca
     2821 tgacatctgc attttctgct ttagctgact ctactactcg tggtgcagat agctgaaagt
     2881 ggtctaatgc gtattgtgtt tcttcattag gtgtacctag agcaactgct tctgtttctt
     2941 tacctaattc attttgcaag tgtgaaaacg caatggctgc cccgattgca tctgtatctg
     3001 gattttggtg accaaaaact aaaatttttg acatgtcatt cgctcctttg tgatttctcc
     3061 tatttaataa tagcaaaaaa acggaaagaa gcaatactta tcttctttcc gatgcgacct
     3121 atcgcgttaa atattttgta tactcttctg tatgaagcaa tcgtttagca ttttcgacac
     3181 gttcttttgt aggtggttcg atcccttcta atggatattt caaaccaagt gtttcccact
     3241 tgtattttcc catcgtatga taaggaagaa tctctactct gtctacattt gacaaggttt
     3301 gaataaagtt gtctagtcgg atcaaatatt catcataatc acttcgctca ggaactagta
     3361 catggcgaat ccaaaccggt ttgttgattt ttgacaaata ttgagccatt tctaaaatat
     3421 tttcattgtc ccaatgcgtt agttttttat gctcttcatt atcgatgtgt ttgatgtcaa
     3481 ataacagtaa atctgtatac ttcatcaatt cttgaaaacg tgaaaagaaa ggttcttcat
     3541 acgtaaaagg ttttccgcat gtatccaatg tagtatgcat cttccgctct tttgcttttt
     3601 taaatagttc gatcaaaaaa tcgatctgca atagaggttc tccaccacta acagtaatac
     3661 cacctttatc tccccagtaa ggtctgaagc gttctgcttt atctaaaagt tcatctgctg
     3721 tgtattcttt ccctccgcca atattccaag tatctggatt gtgacaaaat tggcaacgca
     3781 tgcggcatcc ttgcataaac acgacaaagc gcagtccagg tccgtctact gaaccgaaag
     3841 tttcgataga atgaacatag ccgattgttt tttcttccat ttaagttcac atcttttctt
     3901 tgatttcatt ttaagtacaa gttagctgtc gaagcgaagc tttctgaaca aaatttttcg
     3961 caattgccgc ttcgttatgt gtaaaattta cagggacgga acatacaagt cctgcccctg
     4021 taaatcgttt ctttcaaata agcatactga ttattcgatt ttgatgcgat ggctattcaa
     4081 cgtcctttcg acgctaagtc gaaaaacttt tttcacgaac tttttacatt ctgtcgtgtg
     4141 aagttctcat caagacatct aattgttgtt ctcttgtcaa gtcacggaat ttaactgcat
     4201 aaccagatac ccggatcgtc aagttcggat atttcattgg attttccatt gcatcacgca
     4261 ataaatcatt actgaataca ttgacattca aatgataagc tccttttgag aagtatccat
     4321 ctaagacatt acgcaagttt tcgattcttg tatcttgttc ttttccaaga cctgaaggat
     4381 tgattgtttg ggtatttgaa attccatcca atgcacagcc gtaattcatt ttagctgttg
     4441 agttcaggct agctaataac ccactttgtt ccgcattgta acttggattt gctcctggcg
     4501 caagtggttt tccagcttca cggccatcag gagtatttcc tgtattttta ccgtaaacaa
     4561 catttgaagt aatcgtcaat aatgaagttg ttggttttga gttgcggtaa gttttatgac
     4621 gtttgatttg tcccatgaag tattccaaga tccatgaaac taattcatct gcacggtcat
     4681 catcatttcc gtatttaggg aagtcgccat ccacttcaaa atcaactgct attccttttt
     4741 cattacggat tgcttttact tttgcatatt tgatcgctga caagctgtct gccgcatgag
     4801 agatcccagc aatacctgta gcaaatgtgc gttgcaagtc agaatccatc aaagccagct
     4861 gagctgcttc ataagaatat ttatcatgca tataatgaat gatgttcaac gtattgacat
     4921 atagttctgc taaccaatcc atcatattca tgaagttatg tttcacttct tcaaagtcta
     4981 gtatttctga tgtgattgga cgatattctg gtccaacttg ggcgccagtc acttcatcga
     5041 ctccgccatt gatggcatat aatagtgttt tagccaagtt agcacgagcg ccaaagaatt
     5101 gcatgtcttt tccgacaact gttcctgata cacagcaagc aatcgcacaa tcatcagaac
     5161 cccattgttc acgaagctga ttatcatttt cgaattgaat cgaagaactt tcgatagcga
     5221 ttctgctagc atatgttctg aatccttctg gaaggtgttc agaatacaaa acagtcaagt
     5281 ttggttctgg tgaaggtccc atatttgtta atgtatgaag cagacggaaa tcattctttg
     5341 taactaatga acgaccatct aatcccatac ctgcaatgga taaagtagcc cagataggga
     5401 atcctgagaa taattggtta tattcaggtg tacgcgcgaa tttgaccata cgtaatttca
     5461 ttactaagtg gtcgatcaat tcttgtgctt ctttttctgt gattactcca ttatccaaat
     5521 cacgttggat atagatatca aggaatgctg agatacgtcc gattgacatc gcagcaccat
     5581 tttgtgattt gattgctgct aaatatccga agtatagcca ttggacagct tcttttgcat
     5641 ttgcagctgg tttagaaata tcatagccat atttcgcagc catttctttc atttgcgcta
     5701 atgcacgata ttgttcagtg atttcttcac gtaaacgaat cacttcttca ctcattgtat
     5761 gataaccagt catatcatga tcttttttct tttgttccat caaatagtcg attccataca
     5821 atgccacacg acgatagtca ccgatgatac gtccgcgacc gtaagcgtct ggaagccctg
     5881 taatgatttt gtttttacga gcagcacgca tttcagcagt ataagcgtca aatacacctt
     5941 gattatgtgt tttgcgccag tcagtgaaaa tatgtgtcaa ttcatcgttt ggtgtatagc
     6001 cattgctttt tagcgcattg ttagccatat tgattccgcc gaacggcatg aatgcttgtt
     6061 tcaaaggttt gtctgtctgt agaccgacga ttttttccaa atctttatcc aaatagcctg
     6121 catcgtgtga agtaatagtt gatacgattt ctgtgtccat atcatagaca ccgttacgct
     6181 cgaattggat atcatttaac ttttgcaatt catcccataa tttttctgtt gcttctgttg
     6241 ggccttccaa aaagctgtca tcaccgcgat attcagtgta gttattttga ataaagtctc
     6301 tagtgtctac gccttctttc catttggtac ctttaaatcc attccattgc tccattgtat
     6361 ggacctccta aatttctgtt ttatgtaaca gtttttacgt gattagtata acacagcacc
     6421 ctatctttgc aagggttttc ttgtttttta tttaacaatt tttatgtttt atttacatat
     6481 atttaaatca acgttgacat accaacattt aagcattgtg aacactttca tctttttttg
     6541 caaacgtata aggatttatt aaaaatgaat gaaaaagaga cgccacctgt attaatacag
     6601 atggcgtctc ttcattaaaa tactacaaca gttacgactc gatgaaaatc tcaggagcat
     6661 cgataacttc gatgacttct ccaccttttt tctcatccat cgcaaatgat ccattcgatg
     6721 ttcgatcacc tatagggaaa tcagttgggt caatcgttaa ctgctgccct ttttgattca
     6781 tcaaggttat ttcttttgat cttccttccg acatatagat aatacggtgc ggattttttt
     6841 tcagctcacg catgaccgtc actccccgct tcgcacgtga agtttggctg atttcttgag
     6901 caagcatgcg tttaacagct cctcgctgag taacgattac gatcggcgta tcaccttctg
     6961 ctaacacaag cagcccgttg ataatcctat ctccatcttt caaattcata gacttcactc
     7021 cagctgcctt tgctccaaca acgggaacat ctgttaaagg atagcgcaaa cccattcctt
     7081 gatgcgttac taagaaaaca tctaagcctg gctgatcttc tttcagatac acagcaatca
     7141 gttcatcttc ttccgccttt agcttcatac agctgagcgg acggctctta taggtgcgcc
     7201 atggttcgaa ctctgtcatt cgtgtttgct tgatcaatcc attcttgctg atgaatacaa
     7261 atgttttctc tgcttgaatt ttttgatatg ggaaaacagc taaaatcgat tcgtctacag
     7321 acagattgga gattgtttga gaaatgtgct caccagcatc tttccatttc aaatcgggta
     7381 gttcatggac agggcggtag atcacatttg ctttatttgt gatcagaaga acatggtcca
     7441 atgtactcaa ttcacctgag tatagcaagt agtcgccttc tcgcatcccg atttcttctg
     7501 gtttggaagc actataagaa cgaaggcttg ttcgcttcac atagccttct cgcgtcacac
     7561 tgacaattac gtcttcttgt gccactaaga cttgcgtatc aattttgatt tcttcgattt
     7621 cgtcttcgat tgtcgttaga cgagcggaag aataattttt tttcacttct cgcaattctt
     7681 ttttcataac agagaaaagc tctttgtcat ttgaaaggat tttattcaac tctgtgattt
     7741 gcgcaatcaa gctttcggat tccttttgaa gctctgtgat atccgtgttc gtcaaacggt
     7801 ataattgcaa tgtgacaatt gcttctgctt gttcttctgt aaattgaaag acgtcgacta
     7861 aattcttttt ggcgtctttt ttgtctttac tttcacgaat cgttgcgatt acttcatcca
     7921 gaatcgataa agctttcatc aatccttcta cgatatgctg tcttttttga gctttggcta
     7981 aatcgaattg actacgtttt gttatgactt gctttcgatg ctcgatataa cttcttaaaa
     8041 tgtctttcag tccaacttga tgcggagtca tatggtcgat agcgaccata ttgaaattgt
     8101 aattgatctg tagctcagtg tttttgaaca aataattcaa aattccttga gcattggcgt
     8161 cttttttcaa ttcgacgaca atttgcagac ctgttctatc actttcatct cgtacttcag
     8221 ctattccatc gattttctta ttcaaacgga tctcatccat ttttttcacc aaggtcgctt
     8281 tatttacttc atatgggatt tctgtgatca cgatttgctg tttattaccc ttcattgcct
     8341 ctatctttgt tttcgaacga agaataactt ttcctcgtcc tgtttcataa gcttttttga
     8401 tttcatcttt tccttgcaag atccctcctg ttggaaagtc aggtccagga atatattcca
     8461 tcaatttttc aaggctcgct tgtggatgat cgatcatata gattgtgcca tcgatcactt
     8521 cagctaagtt atgagtcggg atttctgttg catatccagc agatatacct gtcgatccat
     8581 tgacgagaag gtttgggtat cgagctggaa ggactgttgg ttctttttct gtatcatcaa
     8641 aattccacac gagatcgact gtttctttct cgatatctgc tagcatctct ccacttaact
     8701 tggataatct ggcttcagta taacgcatcg cagcaggtgg atcaccgtcc atactcccgt
     8761 tattcccgtg catttcgatc agtacttccc gcaacttcca gtcttggctc atgcggacca
     8821 tcgcttcata aatactgatg tcaccatgag gatgataatt ccccatgata tttccgacag
     8881 attttgctga tttacggaat cctttgtcat acgtattgcc atctttgttc atggcaaata
     8941 atatccggcg ttgcactggc ttcaatccat cacggatatc aggcagtgca cgttcttgga
     9001 taatatattt ggaataacgt ccgaagcggt cacccatcac ttcttctaaa gttaattctt
     9061 gtacttcttg tcgattttcc atcaggtgac ctcctattct acttcaaata actgattgtt
     9121 ttcattttta tctgctcgtt cttcatctaa taaatcatta gaaactgaag gagagatttc
     9181 agtatcctct tttttatcta agatactgcc atcttcttcg agcgtaaatt gcacgtgatt
     9241 ttcgatccat tttcttcgag gttctacttt gtcacccatc agtgtggtta ctcgacgttc
     9301 agcttgcgca gcatcatcga tacggacgcg gatcaacgta cgggaagtcg gatccatcgt
     9361 tgtttcccac aattgttccg cattcatctc tccaagtcct ttatatcgct gcagcatata
     9421 tcctttgccg acttttttaa tcattgctgc caattcgtca tctgtccacg catattcaat
     9481 gacttgcttc tttccttgtc ctttggatac tttatataaa ggcggtaacg cgatatatac
     9541 tttcccagct tcaatcagcg gtttcatata acgataaaag aaagtaagca gtaatacttg
     9601 aatatgggcg ccgtctgtat ccgcatcggt catgatgatt actttgtcgt aattacagtc
     9661 ttcgatagaa aactcaggac cgactcctgc accaatcgta tagatcatcg tattgatttc
     9721 ttcatttttc aagatatctt gcattttagc tttttcggta ttgatgactt ttcctcgtaa
     9781 aggtagaatt gcttggaact ttctgtctct tccttgttta gcagatccgc cagcagaatc
     9841 cccttcgact aaatacaatt catttttctt agggtttcgg gattgtgcgg gtgtcaattt
     9901 tcctgaaaga agagattcgc cttttttacg cttcttccca tttcggcttt cttctctggc
     9961 tttacgtgct gcttctctcg cttctctggc tttgattgct ttacggacca acatctggct
    10021 catttcgcta ttttcctgca aataaaagcc catttgttca ctgatgacgt tatctactac
    10081 ggtacgagca actggcgtac caagtttttc ttttgtttgc ccttcaaatt gcagaagatt
    10141 ttcaggtaca cggatagaaa gtacagcagc taacccttct ctgaaatcac taccttccag
    10201 attcttgtcc cgttctttca gcaaaccaac ttttctcgca tattcattat aagatttcgt
    10261 catcgccgct ttcataccag cttcatgggt tcccccatct tttgtccgaa cgttattgac
    10321 aaaagataaa acattttctg aataaccgtc attgtattga taagcgacct ctacttcgat
    10381 accttctttt tctcctgaaa aataaacaac tggtgtcaat gtatcttttt cttcattcag
    10441 ataatcaaca aattctttga ttccttcttc gtaatgaaaa atttccttga ctggttcttc
    10501 tccacgaaga tcagaaagag agattttaac gccttttaga agaaaggcag attctcttaa
    10561 ccgttcggct aaaatttcat atgaaaattt cgttgtactg aatattgtat catctggtaa
    10621 aaaatggaca gtcgtcccgt ttgctttttt tgtttttcct atctttttca aagtaccgtc
    10681 aggtttaccg ccgtttttaa atttctgctg atactctacg ccatctctaa cgatcgttac
    10741 aattagccat ttcgataagg catttaccac actggctcct acaccgtgca gtccaccaga
    10801 agttttgtac cctccttgac caaactttcc gccagcatgc agcacagtaa aaatcacttc
    10861 aactgttggt attccggaag catgcattcc tacaggcatc ccacgtccgc tatctgcaac
    10921 agttatacta ttgtcttcat ggatggtcac atcaatctca ctgccataac ctgataatgc
    10981 ttcgtcaact gcattatcga cgatttcata gactaaatga tgcaagcctc ggctgtcagt
    11041 ggatccaatg tacatccctg gtctttttct aactgcctct aatccttcca atacttggat
    11101 ggacgagtca ttgtattcgt tgtttacttt ttttgccaag gcaaacgctc cttcaataat
    11161 actttgcggc aaaaccgcac cacgattatc atactagaaa aggtcgtgaa aaaaaagagg
    11221 gtagacgaga aatcaatttt aattatctcc taaccgcttt ttcgacattc ccctctatcg
    11281 atcatctctt ctttgtggtc atttttattt ttggttttct ttcaacttac caagttcaat
    11341 cttaatacaa cgatccatta cgatatctgt acgtcctgct tttcttagca tctcagctgc
    11401 ttctttgttt tcaatcccta attgtgccca gaacacttta gcatcagttt tcaaaaaatc
    11461 acctgctatt tcaggtaaaa attcactccg tctaaataca tctacgatat cgattggatc
    11521 atcgatttct gtcaaattcg catagacttt ctcgcctaga atttcttcac cagccaataa
    11581 tggatttact ggtatgattt tatatccatt gtcttgcaat acctgtgcaa ccatgtaact
    11641 tgttcgttct ggtttattgc tcaagcctac gacggcaata ttttttgctt cttccaaata
    11701 gttgaaaatc gtttcttgtg ttggattttt tacagacatt tctgccaccc cttgcttttc
    11761 tttttattcc atctattata cttgtatctt tcattttttc aattgttcct gttacctatt
    11821 catgctaaaa taaaggatag ttttcaactt gaaagagggt aaaaaatgaa attgatctta
    11881 ttacttattt tagcatatct tttaggttct attccttccg gtgtctggat tggcaaattg
    11941 tttttcaaaa aagatatccg acaacacggg agcggtaata ctggaacgac caatacattt
    12001 cgtgtattag gcaaaacagc cgggattgtt gtcctgctta tggatatttt aaaaggtaca
    12061 ttggctactt gtttgccgat gatcttccac ctttctggga ttaaccctct ttggtttggg
    12121 gtatgcgcca ttcttggcca tacatttccg atttttgctg ggttcaaagg aggaaaagct
    12181 gttgcgacaa gtgcaggaat gcttttagga tttaatcctg tattttttgt ttattcttgt
    12241 atcatcttta tcgttagttt attttgtaca agtatggtga gtttgagcag tatgatcagt
    12301 gcagtattga tcactttatc tacgatcgtc cttccttatg tagctcctgt gattttggct
    12361 aagcccaatt ggttactgac gatcattgcc tttttagttt cttcctttat cttttatcgg
    12421 cataaagaca atatcaagcg tatcagaaac catactgaaa gtcgaatccc gtttggtttg
    12481 aatagcagca agaaaaaatc ctgatacttt ttcattccaa agagataaaa cgggagtgtg
    12541 acaaatcgat gtcacactcc cttattactt acaggatagc ttttttacac accttggtaa
    12601 acggtgttcc ctgctaactt ctcggtcaca acctcttact tcactgtgat ggaatatttt
    12661 gtcttgaaga tttcatgggc acctagttga ttgatcccta ctttttccat caagtttcca
    12721 ctactgttca ccgtgtcagc gattccgcac cacggttcga tacaaacgaa aggcgcttct
    12781 tttggataag gagaccagat ccctacataa ggcatatctt tatagcttaa agttacgcta
    12841 tgggaagatt tttcacttcg aatggcgtac gcatttagac cccttgtttc aaaaaccaag
    12901 gcatcttgct caaaaagttc acgattcaaa gccagattcg tatttgtctg acctaacgtc
    12961 ttttgatcca tatcgatgaa tgggcctttc aatggtaggc gtaatcttga tttcaatgga
    13021 gaaaatgcca gatagaaatc ttcaaatgac aatccttctt caagaggtac attgaaagca
    13081 ggatgtccac cgatagaaaa gaacatttct ttttcaccgg tatttgccac ttgataatga
    13141 accgtgatgc cgtctcctcc taattcatag ctgatcacta attcaaaatc aaatggatac
    13201 ttttctaatg tctccggcgt actccttaag atgaaactag ccatttcttg tccatgttca
    13261 atgacttcaa aatccatatc ccgagcaaat ccgtgttgcc ccatttgata tgttttccct
    13321 tgataaacat actgatcatc ttttaaacgt ccaacaaaag ggaataaaac cggtgcatgt
    13381 ctgccccaat acgctggatc accttgccag atatactcta aaccatgttc ttttgatttc
    13441 agactgatca attccgcacc gtgttcagcg atttcggcga tcagctggtc attttgaata
    13501 ataacagtca ttgccattcc tccaacttcg acttactttt taaaatggga aggctggaac
    13561 aaacatttct atctgcccca gtccccttac ttctaataac tgtgttttgc taactcgtct
    13621 ttaaaacggg aattcaatac ttttaaataa gttcccttca tgcctaacga acgagattca
    13681 atgattccag ctgattccaa ttttcgtaaa gcattgacga tcacagaacg agtgatgccg
    13741 atactatcgg caatattaga agcagtcaga cgaccttcat ctccatccaa tgcttcaaag
    13801 atagcttgta cagccttcaa ttcgctataa gataatgtgt tgattgccat ttggacagcc
    13861 gttgcactcc gtacatctgc ttcgatgctt cgagacttct gataaaggat ctgcataccg
    13921 acaaccgtag ctccatattc tgctaagact agatcgtcat ctgcaaacga ttgttccata
    13981 cgagataaga taatcgtacc caaacgttcg ccagctccaa agatcggaac gacagtcgtc
    14041 agaccaaatg gatatttctc tcttgactct accggaaatg cagtaatatc actagtaatt
    14101 gggatattag cttcagtttt catcaactgt gccactgtat cagtataaga ttcaggaaat
    14161 tgtttctcaa caaacattgt tttgattcgt tcattgttca catcgtgacg ttcattataa
    14221 cctagtaata ctccgtcact tcctatgata taggtgttgc tgtcaagaat accacctagt
    14281 gtgacagcca ttttatcgta aggcaaatct gcttgcatgt caaaagtatt cttttgctgc
    14341 aaaagtgtgt ttatcttgcg ggttttcgtt agcaagtctg tcattgtcgt cctccgattt
    14401 tttataaaat atagcgactt aaatcttcat ttgccgcaat tgagccaagt ttgtcatcca
    14461 catatgcttc tgtgatcgtt atttctccca tttgcatatc tggtgcttca aataatagat
    14521 cttctagtag tttttccaaa atggtatgca gtctgcgtgc gccgatatta tctgtgtcac
    14581 ggttgacatc gaaagcgatt tgagcgatcc gttcaattgc ttcttttgta aacgtaactg
    14641 acacattttc tgtaccgatc aacgcgacgt attgttttac taaagcatta tttggttcgg
    14701 tcagaatctt cacaaaatct tcagctgtca aatcatctaa ttctacccgt atcgggaagc
    14761 gtccttgaag ttctggaatt agatcacttg gtttagacaa atggaaagct cctgaagcaa
    14821 tgaataggac gtgatctgtt tggatgatcc cgtattttgt gtttacttgt gaaccttcga
    14881 caattggcaa tatgtctctt tggacgcctt ctctggatac ttctccagat tgctgtgatt
    14941 tagaagtgat cttatcgatt tcatcgataa aaataattcc actttcttcc gctaatttga
    15001 ttgcctcact atggatatca gcatccttga ctaattttgc tgattcttcc ttgatcaata
    15061 actctaaagc ttctttgact gttaccgtcc gttccacttt tttcttcggt gtcaaagcac
    15121 ctagagtttc atttaggtcg atccccattt gctccaatcc attgttcatc attggtgcag
    15181 gtttcttttg gtcttcaact tcaatggtta cttcacgact atccagtaaa cccttttgta
    15241 gttgttccaa gactgtacgg cgattgattc gaatatcttc tgtcacttct tcttgcggtt
    15301 cagcggtatt ttgctgacta gcattaaaaa gattcatcat ttgttcaaat tgattagtgg
    15361 attgtttctg ttcttttttg ataccaggta ccaagatttt taccaaacgt ttttctgcat
    15421 tttttttagc acttgtgtat acacggctgt actgctcttt ttctacgatt tggatcgcat
    15481 tttctactaa atcccgaacc attgattcta catctcttcc gacataaccg acttcagtaa
    15541 attttgttgc ttcgactttg acaaatggtg cattgacgat ttttgccaaa cgtcgtgcga
    15601 tttctgtttt accgacccct gtaggtccga tcatcagcat attttttggt gtaatatctt
    15661 gttgcatttt ttcatctaac tgcaaacggc gatagcggtt acgcaaagca acggcaacag
    15721 atttttttgc tgcgtgctgt cctacgatat attcatctaa ttcacttacg atttgtcttg
    15781 gtgtttgttt taattcatac atatctactc acctcttaca gttcttctac gataatatta
    15841 tgattagtaa agatacagat atctgcggca atatttaacg cattttctgc aatttctttc
    15901 gcactcatgt cgttttgagc atagtttttc attgctcggg ctgcagccaa cgcaaaattc
    15961 cctcctgatc cgatagctaa gataccatca tctggtgtga taacttctcc tgtacctgaa
    16021 acaagaagca tttctttatc attcattacg atcagcatcg cttcaagttt ttgcatagat
    16081 tgctgtgtcc gccattcttg agctaattcg acagctgcgc gttgtagatt cccgttatat
    16141 tcgtttagtt tgccttcaaa tttttcttct aaagtaaacg catctgcaac acttccggcg
    16201 aaacctacaa caacttctcc attgtagata cgtcgtactt tctttgcagt gcctttcatg
    16261 acaacagatt cacccattgt tacttggcca tctccagcca tagcaaattt cccgtctttt
    16321 tcgacggcac agatagtggt tgaatgaaat ggtgattcga ccatgtttgt ttcctcctaa
    16381 atttgttctt ttccttattt ttacgctctt ggatggaaag aacgatagtt tttttgtaaa
    16441 ctttcttttg ttacatgggc atagatctgg gtagtcgata aattggcatg tcctagcaat
    16501 tcttggactg tccgcaaatc tgccccatta tttaataaat gagtagcaaa tgtatggcgc
    16561 agcatgtgag gatggatatc actatccaaa ctgctttttt tgatcatttg attcaagaca
    16621 tattcaattc cagtaggcgt gattggtttt ccatgatgat tcacaaaaac gatatcatgc
    16681 tcttcgtgat attttgtcat caatacctgg cggccttccc tgaaatattc ttgcagagca
    16741 tcttgagcaa aagaacctag aggagcatat ctctctttgt tccctttacc atggatcaac
    16801 atgacctctg aatcaaaatc aatgtctgat accgacaaac tgctacactc acttaaacgg
    16861 atgcctgacc cgtaaaggac ttccagtaaa gcgcggttcc tcaaatccaa aggactatct
    16921 cctgctacgc tgtcaaaaag tgcttgcatt tcattttcat aaaaaaaacg tggcagtctc
    16981 aaatttttct ttttgagatg aacgtaagaa aatggatttt cttttatcac ttcttctttg
    17041 agaagatact gataaaacga acgtaggctg gctatcttgc gactgataga atttctgcta
    17101 tagctaggac gatgatcagc taatgcactc aaatagacac gaaccgtaaa atattccgcc
    17161 ttcagcagat cagcgtctcc agattcctcc aaaaaatgtt tgaaatggag aatgtcttct
    17221 tcatatgctt ctttcgtttt ttctgaatat cctctttcga caatcaaata acgcaaaaat
    17281 ttttgttccc acgtggcttc tgtcattttc tcaccttcct tttgtcccag agaataattt
    17341 agcacagcgt ttataaaaag acaaataaat tgtcagaatt taaccaatat ttctaagttt
    17401 gttcacaatt ggcgcaattc ctcacatttt gtttgagaaa ttcttaagaa aaacgcaaga
    17461 aaaagagcga gactatccgt ctcaccctct tcatggagtt tccttactca tttgggattg
    17521 cagttttttc taattctgtt tttattgcct cattagcttt taaagctcgt tgtgcaattg
    17581 cttcatatct tgcttttttg tctcgaatgc gctctggcag ttcaggaaac agaccatagt
    17641 tggcattcat cggttggaaa tgttttcctt ccgcatgagt aatgtagtaa gccatacttc
    17701 caatagctgt ttcttgtgga aactcgactg gttccatttc ttttgctaag cgtgcggcgt
    17761 tgattccagc aagtagtccg ctcgctgcac tttcaacata gccttctaca ccagtcatct
    17821 gacctgcaaa gaacagatct tcgcgtttcg ttgattggta agtcggtttc agcaattctg
    17881 gcgaattcat aaagctgttt cggtgcatca caccatagcg aacgaattca gcattttcta
    17941 acccaggaat catccggaaa acacgttttt gctcgcccca tttcaagtga gtctgaaagc
    18001 ccacgatgtt gtataaagaa gctgcagcat tgtcttgacg cagctggata acagcataag
    18061 gacgtttgcc agttttagga tcttccaatc caactggttt cataggtccg aaaagcatcg
    18121 ttttgaatcc tcgctgtgcc attacttcaa ttggcataca tccttcaaaa tatttttctt
    18181 tctcaaaaga tttaagttct gccacttcgg cattgattag tgcctcatgg aaagctttga
    18241 attcctcttc tgtcatcgga cagttcaagt aagctgcttc tcctttgtca tagcgggact
    18301 tcaaatatac tttatccata tcgatcgtag ctttgtctac gattggtgca gctgcatcat
    18361 agaaataaaa accatctgaa ccattgaatg ctttgatgct ttctgctaat ggatgagaag
    18421 tcaaaggtcc tgttgctact acggtgattc cttccggtat ttcagtaatt tcttcgttta
    18481 tcactgttac taatggatgc tctttgactt ttcgggtaat tgtttcagaa aaagaatcgc
    18541 ggtcaacagc taaggcacca ccagctggca ctgctgtttc atctgccgaa gtgattacta
    18601 ccgaattcaa tcggcgcatt tcttctttca ataaccctac tgcattagcc aaactgtttc
    18661 ctcttaatga attggaacat actagttcag caaaattact tgtgtgatgc gcctcagtcg
    18721 atttgactgg acgcatttca tataaacgga cagggactcc tgcttgtgcg atctgccaag
    18781 cagcttcact tccggcaagc cctgctccaa caacatttac cattttcaat gaaagaacct
    18841 cttttctatt atttttggat gtcttcttcg taatctccat ttacacagac tacttgttta
    18901 ccgcctttga ctttcttctc gaccagatat ccgtcacatt ttggacaatt tctgcccact
    18961 ggtttatccc aagaagtgaa ttcacattct gggaaacggg aacaaccata gaatagtctg
    19021 tttttcttag attttcgttc aatgacttgt ccttcatgac atactgggca agtcacaccg
    19081 atttctttta cgattggttt cgtattccga caatcaggaa aattactgca agcataaaac
    19141 ttcccatatt tccctaattt tatcaccatc ggatgtccac agacatcaca atcgaatcct
    19201 gctggttcat ctttgatttg aattttttcg attttttctt ccgcattcgt cagttctttt
    19261 tcaaacggtt tatagaaacg atcaaccact ttgacccacg cttccgtgcc ttcttcaatt
    19321 ttatccaaat ctccttccat ttctgctgtg aaatggatat cgacgatttg agggaagaat
    19381 tcacagatta atgaattgac aatttctcct agttcagtcg gctcaaatct tttttgtgtc
    19441 aattttacat agtagcgtct ttggatcgta tcaagtgttg gtgcataagt ggaaggtcgc
    19501 cctaccccgt tttcttctag tgcacgaatc aacgtagcct cactaaaacg tgctggtggt
    19561 tgagtaaaat gttgttttgg ttcaatatca acagattgga ctacatcgcc ttcttcaagt
    19621 tctggaagaa tattttcttt ctcttctttg ccgtcatctc taccttccac atacacttgc
    19681 atgaatcctt tgaatttgat ttttgaaccg ttcgcaatga agatgacacc attttgttct
    19741 aatgtcactt tcattgtatc taaaatagct ggtgtcatct gactagccac aaaacgtgac
    19801 cagatcaatg tataaagttt caattgatct ttatctaaat attgtttgag ttcatctggt
    19861 gtacgaagca cactcgttgg acgcacagct tcatgggcat cttgtgcgcc ttgagcattt
    19921 ttgatctttc ggtgatcatg tctagaaaat tcttttccat acgtatcttc aataaatgct
    19981 gcggcctctg cttttgctgt atcggcaatc cgggtagaat cagtacgcat gtatgtaatc
    20041 aagccgactg ttccttgttt acccaaagtg attccttcat ataattgctg tgctaccatc
    20101 atggtttttc ttgttcgaaa gtttaactta cgggcagctt cttgttgcag gctacttgta
    20161 gtgaaaggat tcgcagggtt tcttttgcgc tctttctttt ctactttttt aacttcgaag
    20221 tctgttccat caattctagc agtgacttct tttacacttt gtgcatctgg taatttcttt
    20281 ttcttaccgt cgattcccca gaaatttgct ttgaatttct tgcgagcttt tttaaaatta
    20341 ccgtcgatgc tccaatattc ttctggaaca aattctcgga tctctttttc acggtcaata
    20401 ataattttta aagcgacaga ttggacacgt cctgcactta accctttctt gaccttacgc
    20461 cagagaatag gactgatcga ataaccaacg attctgtcta aggtacgacg agcttgctgt
    20521 gcatctacta aatctacatc gatcgttctt ggttccttaa aagctgcttt gactgcgtct
    20581 tttgtgattt cattgaagac cacccgattt ttttctttca gatccaaccc aagtaggtac
    20641 gctaaatgcc aagcaatcgc ttctccttct ctatccgggt cacttgccaa gtaaactttt
    20701 tgagcttttt ttgctgcggc tttcaggctt ttgatgacat cgccttttcc gcgtatagaa
    20761 atataatggg gttcataatt gttttcaaaa tcgataccca ttttactttt cggtaaatct
    20821 cttatatggc caacactggc cattacttta tagtttcgtc caagatattt ctcaattgtc
    20881 ttggcttttg caggtgattc tacaatcact aaatatttat acgccattac tgaatggact
    20941 cctttccatt gtcgtgttcc tactattaaa agaggattca tacaaatcgt agctaatcat
    21001 atccattcag aaaacgtttg tcaagaaaag caaatgaaaa gtatggtgtt attctttaaa
    21061 ttctgccaag atatcttcac ccgaaaccgt gcaaactgca ccgtcttgga tcaaatgatg
    21121 acatccggta gaatgttctg ttaaaatatt tcctggaaca gaaaaaactg ttcttccaag
    21181 atccaatgct tgctgtgctg ttatcagaga gccgcttttt ttcttggctt caaagacaca
    21241 tgttccttta gacaaagctg cgataatccg atttctcatt ggaaaacaat gctttttcgg
    21301 gccggattga ggaggatatt cactcaaaag aagatgatca tttgcaatca cctcttgcaa
    21361 gtcacgattt tcagcaggat agacttgctc tatccctgtt ccaatgactg cgattgtccg
    21421 tccaccatga gagattgctg cttggtgacc gtatgtatct atccctttcg cgcagccact
    21481 gacgatcaca tatccagctt ctatcactgg ttttagtaaa taataaactg tttttttggc
    21541 taaagcagaa gtgtgtcttc caccaacaac gcttaacatg ttacaagaaa gtaaagatag
    21601 atcaccatta taaaacagta caattggcgg ataggtcaag tgaagcagct gagggggata
    21661 tttgggatca aagcaagtga taaacgactg tttcgattgg attttgcccc agttagcgtg
    21721 aatttcctgc caacttttgc gaaacagctc ccggtgtttg gttattttac ctatcttaat
    21781 gatgtcttca aatccgattt ctttataagg atactgcatc atccagttag caagtgccca
    21841 ttttccttga ctactgattc cttttgtata agcaagtttc aataaatttt cttctatttg
    21901 atacatcata aaaccgcctt tcgtctctct ttctaataag atacgtaaaa gcggctgttt
    21961 tttattgtaa atattttttt acaggtgcaa aacttttacg atggattggc gtaattcctt
    22021 gtcgttcaag tccttctaag tgttcctttg ttccataacc tgcattatgc gagaaaccat
    22081 atccaggata aagcacgtca tattctttca tcaattggtc tcgatatact ttagcaatga
    22141 tacttgcagc agcaatcgac acagatcgtg catccccttt gattattttt tcctgctgaa
    22201 taggcaaatc caaggtcatc gcatcgatca acaaatagct tggtacaatg gacaattttt
    22261 ctactgcttg gatcatcgct aatttacttg cttcataaat attcacatca tcgattgtct
    22321 gctgatcaac gatcccgatc ccgattgcca gggctttttc ttgaacttgt tgataaagaa
    22381 gttctcgctt tttctctgat aattgtttgg agtcattcag ccctaatatg atctcatttt
    22441 tcggcaagat aactgcagct gccacgacag gtccagctaa tggtcctctt cctacttcat
    22501 caatgccggc aatccattgg tgaccttgtg cgtacccttg cttttcaaag caaaacattt
    22561 cttcatattt ttccttaatt aatgcttcct ttttcaatcg tcgttcaaaa gaagccagtg
    22621 cttgctgcac gcctttgcgt ggatcatttc ttaatgattc tagtgtagga tcatctattc
    22681 tctgtatgct tttcaatcgt tctttgatac tttggatgga ttcagtcatc acttttttgc
    22741 tccatttctt cgcaacgatc cagtgtgtat cgtccaagtt ttccttgacg aatatcatgc
    22801 acgactaatt cgctgccacg atcatagtct tctttatacc cacgttttgc tgtaatatcc
    22861 atcagtaatt ccggtgccaa tttttcttct tgctcgattg ataaaccata acgatcgctg
    22921 attcttcctg aataatatcg ggcaaaaaag tctaatccgt aaatcgctaa atcatctaaa
    22981 tggacaagct ggtctttgat cgcgcctgtc agcgctagtt ttttgccgat ttcttcatct
    23041 tcaaatttgg gccataaaat ccctggtgtg tctaacaatt ccaactcatt acctaaacga
    23101 agccactgct gaccttttgt tacgccaggc ttatttcctg tctgtgcaat tttcttgcct
    23161 actagtcgat tcatcaacgt agatttccca acatttggaa tacctaagac cattgcccga
    23221 atagcgcgag gcttgatccc ccgagcagca tctcttgccc gtttttcttt caatgcttct
    23281 ttagccgcag gtactagacc tttaattcct ttactttctt gtgccgtgat agctaatgca
    23341 agatggcctt gtttacgaaa atattcttgc cacagctttg tctgttgtgg atctgccaga
    23401 tccgctttat tcaataaaac aaggcgaggt ttttgctgaa tgatctgatc tagcattgga
    23461 ttacgagaag ataaaggtaa tcgtgcgtct accaattcaa atacgatgtc cacatatttc
    23521 attttttcag aaacttctcg tcgggctttt gccatatgtc ccggaaacca ttggatggtc
    23581 attgttctct ccattccttt cataccggaa tattccccgg ctttacttgt ttttttcttt
    23641 ttcaatataa attcactttt ttattataaa gaaattccat ctattagtta agtgaaagtg
    23701 aaccagtcct cattctatca catcttcact tttttgatga tgcaaatttt tattttggtt
    23761 tctatatatg aatctaaatt tttgacagcg tttcctaaat aagagatgat agacgaacaa
    23821 aacagaaagg agacgaaaca aatgaatagt atggtttttg ttaattttcc tgttgctgat
    23881 gtcaatcgtt ccgttgcttt ttatgaaaag ctgggtttta aaaaaaatga agaattttct
    23941 actgaagaag ctggttcaat ggtatgggat gacacttttt ggataatgct tctctctcat
    24001 gatttttata aaaaatttat aaaagataaa gaaatagctg ataataaaaa aacaagtggt
    24061 gctttgattt cgtttagtgt tgaaaatgcg gatgaagtaa aaaaatttgc tcaaattgca
    24121 aaagaaaatg gtggtacttt ccacgaagta gatatgggaa tgcctgaaga agaaatgttt
    24181 tcattagaag tccaagatct agacggaaat acattagaac ccgtatggat gaagatgtaa
    24241 gcagataact aatctgacgt tttttataac aaaaaaacaa aggcggatga tacgataagt
    24301 attctccgcc tctatttttt ttattcgtac aatctctttt ttacagttta taaaagcagt
    24361 cttctcaccc tttttttaat tgttagatgc cagtgtatgt aaatcaccaa tgtgtcatga
    24421 cacattggtg attttatggt atccttttat tattaactga caaaggagag aatattttga
    24481 acactttgat tgcaaaagac gtttttagat tgcccgaaat gaatgcaaga ttgttgtcga
    24541 cctctgctct tttattagcc cttgatgtca tcttatacaa gatagcgatt ggtcctgctt
    24601 attttcaaat cacattcgga tttttaagta tggcgcttat gggttatcta tatggaccga
    24661 tctgggctgg tttgcttgaa atgttgtcga acactttaaa tttcacgata tttggttcag
    24721 gaacgtttca agctgctttt cttatcacgg ctttccttgg tggatttatt aatggcttat
    24781 ttttatatca taaaaagata aacctgcctt ccgtcatcgc tgcccaatta ttgattatga
    24841 tcccagtcag cctttttatc aattcttggc tgatgtctgt tttgtttggt gcagatttca
    24901 aagtaatttt tctaggtaga ttcatcagga atatgatcca aatccctatt caaataatgg
    24961 ttgtttattc atttatcaaa actttagagg ctagaaaagt gtttaaacga gctgtttgat
    25021 tttacgtact aaggtagtaa actgattttg gattaaacaa taaagcgtgc agtctcaaca
    25081 gactgcacgc tttattgttt ctcactggat ccattttatt tcatcaagtg gataatagac
    25141 aaatcttgct ttacctagaa tatcagtttt attgatcgtg ccaaacgaac gactatcttt
    25201 tgaaactctt cgattatctc caagtacaaa ataattatct tccggaagct tggattgtcc
    25261 cgttagctct tgtagtgtaa agtccgtcgt ataggatgca tttgcatgat ctgatttcag
    25321 gtttttagtt agataaggtt ccttaactac tttcccattg atcttcaatt gatcattttg
    25381 gtagctgaca ttttctcctg gaagaccgat tactcgtttg atataaatcg tgccatctgc
    25441 taattgaaaa acaacaatat caaatcgtct gatctccgaa aatttttcca tcacaaccat
    25501 atcaccttgc ttcaacacgt tttccattga attgccttcc actggtaccg ggatgaataa
    25561 aaatccccgc aacatgaacg caaggaatac agaaacaagt aagtatttga atacaagcca
    25621 gaagcgatta atatagcgtt gtttttttgt caatctctat caatccatcc ctcttttttt
    25681 cattctttct ttattataag aaaaatatca aaaaaatgcg actagatagc tgaaataaaa
    25741 ataattcttt caattaggct attggacaaa aaactttttt gatcataaag tacaaaacta
    25801 tctcttatat gtcatgatta ttttctaaat atttttccca tgcttcgtca aagatatcca
    25861 tggacggcaa atagtcgaca ttcagttcca gataattaga aatttcttcg tagctagagc
    25921 tgtgcttagg aaattggatg tctttaccgg cttctgtagc gaaaacagat aaagcatcat
    25981 gtgtgccacc gcctcgcagt gtcattaaat aatgaaaaaa gcttcgattc acccttcgtt
    26041 cctccaattt tcttcgataa aggcagcccg gcgtgcatgg tttcttttaa aattttcagg
    26101 atgcttttta taatactctt gatggtcttc ttctgctaga taaaatggtt gggctggttc
    26161 gatttttgta acgattggct gttcgaaacg tccactttca gctaactgtt tcttactttt
    26221 ttcagcaatg atccgctgtt cttctgtcaa ataaaagatc accgggcgat aattgtcacc
    26281 gcgatcctcg aattgtccga aagcgtcagt tggatcagtt tgctgccagt aaagttctac
    26341 taattgttca tatgaaatca atgttggatc atagataatc tccactgctt ctgtatgtcc
    26401 agtcgtttgg ctttttactt gttcataagt cggatctggt acatgccctc cagtatatcc
    26461 tgacatcact gtatggatac caggaagcgt gtcgaaaggc tggatcatac accagaagca
    26521 gcctcctgca aatattgctc gttccattat gattccccct ctaatactac tcatccgatg
    26581 attttttatt ttcttttgtc ttgctttcag tactgctggt gctagttttg ttggtatttg
    26641 agttgtttga agcatcagag gtatcttttg gcaaatatag actaaaccga atgtcatcat
    26701 ttactaagtc gattttatct gctttaatat acatcccgtt ttgcattcgg aattgatcca
    26761 acctaagcat gatagtcaga tcatctgtat tgacttcgat ccaatctggt aatttatagt
    26821 ttctcttgat catattcatc acatctttga ttggcaaact caatgttccg atagaaagac
    26881 ttctagcttt tagttggaca tttccgtttt ccatcacata cggatcaaaa tataaataga
    26941 aatcgatagg aaaacctagt acttcgaaag taccattgag tagtgcttca ttttccaaat
    27001 aaaatttata ttcaatatca ctatcttttt gatactcact aaggaaaaag ctgatgattt
    27061 gattgacttt ttctttattt gaattgatcg ttaagacagg atctccttgt ctttccgtaa
    27121 gagctggtat ttctgggagt tctggttccc gattagcaaa aatacggctg cctaaaaata
    27181 cggtcgaacc aagtactacc ccaagaagga taaggaaggc gaatttccag taatttgttt
    27241 tctttgtttt gtttgaagat acgtcagatc ttgttttcat gtttattgat catcctcctt
    27301 ctctaaccat tggttttgtg tctcgatcag ctgatctctc actgcatttg ccatcaattg
    27361 ataacctaaa ttatttggat ggaaatgatc ttcttcatag agcacattat ttttgatgcc
    27421 atttagattt tctgtcgtgc tagttgtttg atctgttcct tcttctgtga cacctacttc
    27481 ctgattcaat ccttgataaa gcaaatcatt gattgggata aagtagcagt tcggcatttt
    27541 ttttgtcacc gtttctgttc ctttgttcca attgttaaca attgtctgca tgtcagtgat
    27601 ctcaggaaag ttcaaataaa atggattgta gatgcctaaa atgtaaatag gtgcggtttt
    27661 attgagggat cgaaattctt ccaatagttc tgtgacattt tcctgatagt ttttcagtgg
    27721 ctttttaaag gaattgaccg ttaacccgaa taaattattt tggataactt tcatcaaatc
    27781 attaccgcca acagtcagtg tgataaaatc agctgttttg atattgtttc gaatttcttc
    27841 ttttgttttt actcgcttta agatctggtc acttcgttca ccatttacac catagttttc
    27901 tgtttcaacc gcggtcaaat tataacggtc ttttagatca tttgcgacaa ggggaacgaa
    27961 tcctccttgc ttggtttgat ctcctactcc ttcagtcagc gaatctccga tagctatgta
    28021 gtgaagtttt tcttttttat ttggatcttc tctcacagta gttactggtt tcattagtgg
    28081 gccagctttc ggtatagaag cagctaaacc tgcatagatg atgatcgcaa ttaataatgg
    28141 gaacaaaacg aggatcaaat gtcgtttcgt taatttcatt ttttcacctc ttgtattcga
    28201 ttggttctaa tgttatcaaa aattttatca aaaaggtagt ctttatgaaa aaagaaaaca
    28261 attattttcc taattttata aagaaaaaga gaataagctg caagcctttc aggctgaggg
    28321 cttatcctct tttcgactaa tctacataat acatgattgc aaacgcgtct ttaccagtat
    28381 gcgtagcgat aaccggattc gtatgcagta cagggatatg catgtccggg aataattctt
    28441 gcaaaccctc tttgaattct tgcgccaatt cttttgcatc cgcatgtgaa ataccgattt
    28501 gtcgcacatt tggcaattct ttcagttcat ttttcaaatt attaaaccat ttatcgaacg
    28561 ttttctttcc tcgcccctta gttacaggga ttaattcaga atgggcgaaa ttcatcacca
    28621 cgcgtatgtt caaaacacta gacagcaaac cagttgttcg gctgattctt ccacctttta
    28681 ccaagttatc tagtgtggat attccgatgt ataattttgt attgtcttgt acttttttga
    28741 cttcttcaag gattttctct actgttttac cttcttttgc taattcagct gctcgaatta
    28801 cctgaaaaga taatccttgg tcagtggtat cactgtcgat aaccgttact ttcgtttttg
    28861 ttagattact ggcttgtctt gctgcttcta ctgttccact caagcctttt gtcatatgga
    28921 tcgaaatcac ttcactgccg tcttctccta gctcatcata catcgagatg aattctccaa
    28981 ttggtggttg gcttgttttg ggcagatttt ttgctttcgc catcatttcc ataaatcttt
    29041 cgccgtctag ttggtcatca tcgggatata ctgttccgtc gatcatgatg gatagcggca
    29101 tgacacgaat gcctaaagca tcgcgtacat ttttttccat cgtacatgaa gaatctgtta
    29161 cgatttttac atttgtcatt attttctctc tctctttctt taacaagatg ccttattcat
    29221 gttaaactac atgtaagaca atttctattt ttagtataac agacaaataa tgatttttca
    29281 ttaattacac ctagtaaagg agccgttttc atggaaaaaa cgaaatttaa tcgaacgtat
    29341 gaaatattaa atgaagtgtt taatgcagtc acccatggaa ttgggactgg attgagtatc
    29401 gcgggactag tggttctttt agtaaaaggt gcccacttgc attcctccct ccatattgtc
    29461 tcttatgcta tctatggatc gatgatgatt cttttatttt taacgtctac tttgtttcat
    29521 tcattgattt ttacaaaagc aaaaaaggtg ttccaagtat tcgatcatag ttcgattttt
    29581 cttttgattg caggaagtta tactcctttt tgtttgctga gtatccgagg atggctaggt
    29641 tggactttat ttgtattaat ctggttgttt gcgattcttg gcattatata taaagcgttg
    29701 acgttgcata aacgggagag agtatcaaaa gtatctacga tcatctatat ttttatgggg
    29761 tggctctgtg tcgtggctgc tgttcctctt tatcactctc ttggaatgac tggtgttatt
    29821 ttgatggtag caggcgggct ttcttacacg ataggcgcat tcttttatag cttgaattct
    29881 atccgctata tgcatgttgt ttggcatttg tttgttatgt taggtgcagg ttttatgtac
    29941 ttctctgttt tattctttac ttaataaaaa aaataaagct atggatggga cagctctaaa
    30001 taacagctag tctatccata gcttttttat atcgtcaatt cgcctgtccc gctttttaca
    30061 ggattctcat tacgctgata cgtttcaaat tgatgcggat aacgattttt ttcatccatt
    30121 tctccttgac tagtattgat cagtgaccaa tcagaccagt caacaggtgg aaaataagca
    30181 tctccatcga atacatgatg gatgactgta cggtataata tcgtacaaaa tggcagaaat
    30241 tcttgataaa cagctgaccc tccagcgatg aaaaagatac cgtctgtttc tttttccaga
    30301 gcaagcacgt cttccacact atgcatgaca tgcacgcctt ctgctttata ggaagaatct
    30361 ctcgtcaaaa caatcgtctg acgattcggc aacggacggc tacccatccc ttcgaatgtt
    30421 tttcgtccca tcactagtgt atttgcttct gtcatttgtt tgaagaattt cagatcattt
    30481 ggcaaacgcc aaggaagacg gtcttctttt ccaatcaatc cgttttcatc ctgtgcccaa
    30541 attgcagcta acattttctc atcctttctg attctaaaca gcgataggag ctttgatcgc
    30601 cggatgtggg tcatatcctt ctaattggat atcttccatc tcaaaatcaa atactgactg
    30661 cttttcctga ttcaaaacta aagtcggaaa tgcgcgtatt tctcggctca attgttcttt
    30721 catttgttcg atatgattga tataaagatg agcatctcct aatgtgtgaa caaattcgcc
    30781 aaccgctaaa cctgtttcat gtgcaattag gtgagtcagc aacgcataac ttgcaatgtt
    30841 gaacggaacg cctagaaaca catccccact gcgttgatac aattggcagc ttagccgtcc
    30901 ctcttgaaca taaaattgaa acatcgtatg acaaggaggc aaagccatgc taggaacatc
    30961 ttccggattc catgcagaaa cgatcaatct tctagaatca ggagtctttt tgattgcttc
    31021 gatcacttgt ttgatttgat cgataaagcc accgtctttt gtttcccagt gacgccattg
    31081 atagccgtag atgtgtccaa gatcgccgta tgtttcagca aaggaatcat cggttaggat
    31141 cttctcgcaa aaaagcttgc tctgcttttt atattcttca ttgaattcag gatctgtcaa
    31201 acatctatgg ccaaaatcag tcatatctgg tccattatag tctggacttt tgatgtagcg
    31261 ttcaaatgcc cattcatccc aaatatggtt tttattttgt aatagatagc ggatattcgt
    31321 atctcctttt aaaaaccaaa gcaattcgct tttgatcaat ccaaaaggta ctcgtttcgt
    31381 tgttaacaaa ggaaaccctt tttgcagatc aaaccgcatt tggtatccga ataagcttaa
    31441 tgtgcctgtg cctgttctgt cttctttacg atgcccttct tccaacaatt ttcttcctaa
    31501 gtcaagatac gcttgttcca tcctttttcc tcctaatctt ctacccattc actcaagtat
    31561 tcccaccgtg ccattttttc ttcaagttgt gcttctgttt cagatacgtc gttttgcaat
    31621 tcttgtaatc gagtgaaatt atctccttga tgattcatct cttcttggag caatgagatt
    31681 ttttcttcaa gttctgcaat ctcatcttca atcgtttccc attctttttg ttccatgtaa
    31741 gataatttct tcttttcagc aggttccttg ctctgtattt ttggtttcgc tttttcagca
    31801 ggctgctggg tctctttttg cttcagtaaa taactggaca tgtttccgta ataaagttct
    31861 tgttttccgt ttccttggaa tactaataac ttatctgcta ccttgtccaa gaaataacgg
    31921 tcatgggaaa cagtgatcac agcacctgga aaacttctaa aataatcttc caaaatcgtc
    31981 aatgttgcta tgtccaaatc attggtcggt tcatccaata ataaaacatt aggttgctga
    32041 atcagcagtt tcaacaaaaa taaccggcgt ttttctccgc ctgaaagttt gcctatcagt
    32101 gtcccatgca taaatcttgg aaataaaaat cgttcaagta attctgctac tccgatttga
    32161 ctgccatctg ctgtttttgc ttcttcagca gcttcttgta agtaagcaat catccgttta
    32221 tctggtgcca tttcttcatt ttcttgtgta taatacgcaa gatgaaccgt ttcaccgata
    32281 gacattgtac cgctatccag gggaattctg ccagctagaa tattaagcaa tgtcgatttt
    32341 cccgcaccat ttttaccagt aatacctagt ctttcccgag attgaatcaa taaatctaaa
    32401 tgttctagca gtgtttggtc atttatacgg taactgccat ctttgatttc cagtactttt
    32461 ttccctaaac gttgtgtcgc taggttcagc tctaaatcat cttcttggtt tacttggtac
    32521 aagttctcct ttagatcttc gaaacggttg atccgtgctt gctgcttggt gccacgtgcc
    32581 tgtacgccag ccctcatcca agctaattcc tgtttataaa gccgcttgcg cttttcttct
    32641 tgttctactt ctacacgatc acgttctgct ttttctaaga cgtaagcttc ataatttcct
    32701 ttatactcgt ataattttcc aaatgaaagt tcgaagattc ggtttgctac tctatctaga
    32761 aaataacgat cgtgggtaac catcaatagt gcgccacggt attgtttcaa atagttttcc
    32821 aaccactcga tcgcttcata atctaaatgg tttgttggtt cgtctagtaa tagtaaatcg
    32881 ggttcatcaa tcaacacttg cgccagacta actcgttttt tctgaccacc tgataaagta
    32941 ctgatttttt tatctaattc actgattcct aatttttgca aaataatctt tgcgttggta
    33001 tctgtagtcc aagcatcttc tttattcatt tgttcttctg ctttagcata acgtttttgc
    33061 acatcagggt cttctccgtt ttcccctagt tcgatcaatg cttcctcata tgctcgaaca
    33121 gtttggatca aggggctgtt tccttgaaag actgcttgga gaacagttaa ttcatcggaa
    33181 aattcagctg cttgagaaag ataaccaata cggtaatcag ttggataaaa cactgtttgc
    33241 cgatcaccgt cgctgctatc gataccggca ataatattta agagacttgt tttacccgtc
    33301 ccgttcgtcc caatcaaccc gatacggtct ttttcgtgga ccaaaaaaga aatctggtca
    33361 aacaaatctt tttcaccata agtttttttg aggtcagtta cttttaattc tttcaacatg
    33421 ttccctcctt atcttggata ttgtatcata aaaatagtga acttcaggaa aatttttttg
    33481 gtatttgttc tttttgtgtt ctcaaaaaaa gaacaggacc gattcgatcc tgttctgata
    33541 tgttttgttt tttataaaac ttgaaaattt ttaccttttt attttattgg actcgttttt
    33601 tttgactttg ctcgaaacgc aaatcggaaa gaaaagttat cagatggttt gttcatcgta
    33661 taagattcta acaaattgat attcctgcga tagctgttcg cagaaaacgt cgtgattttt
    33721 tcagcaactt cgtattcatc aatgattttt ctttcaagat aataacctct tagcattttt
    33781 ttcgtaaaca gactatcttg ctggatcatc cacgaagaaa tcaaattccc cgagttcatt
    33841 tttattcctt caagtttgcg ttgctggata aaaaattgaa ccatcgtttc atcataaata
    33901 tcagtcaagt tatctaaact ttcaataatc aactgagtat ttttttgata tgtctctctg
    33961 atttcttgga tatcttcatc actaataaat gtctgacgat cagcatatct tctttgccaa
    34021 aacattttcg gatggagaac gattcgcatt aacctacgga caacaatgaa ccaaaagccg
    34081 ataaaagaca aaatctggct tgtgatagaa tgttcgaatc gagaaagaaa acgggagtaa
    34141 aaacgataac cattagaaga aagcttgccc gaacgataac tttcatccaa tccatcccgt
    34201 tcgatcgaca aaatcagtgc ttgtatttct tgtacttctt gcttttcatc atcggttaaa
    34261 tcttccaagt acagatcacg tatccgttct tgataatttt ctatcactgc ttcagtggct
    34321 aaaaattctt ttgtattacg atcaatttcc tttatttctt ttcgcaaaac ttcaacgacc
    34381 tcttctaaaa tcatcaaagc attcaaatcc gtgcttttcg cttcctctgt ttccgtcagc
    34441 ataggcaaga caatgatccc tatgaccaaa gtcactaaga tgacacccgc cgtcagaaac
    34501 aaaagcaaag accgctggta aaacatcata ttattgattg aaaatggcaa aataaaaatc
    34561 gtagccaaac tgactgttcc ctttacaccg ccaaatgtta agatcaagat ctcgttcaac
    34621 tgttttttga actttttact gccatctttg aatacataaa aaagactgat agaaatgaat
    34681 ctgatcacaa aaagcatgac agagattaat acgataatcg ctaataaaag accgtttgga
    34741 tataaaccgt ctccccatac tggcgagaac acttgcgtca attcgattcc taaaaatata
    34801 aatactaatg cattcagtgt aaaagcaata gttgtccatg tactgtggga caagctcgac
    34861 aattctgcat caaacacaga gattttccta aaaccagaag cttggagaat acctgctgcc
    34921 actgcagcaa tgattccaga tacatcaaat gcctctgcca atacataggc tagaaaagga
    34981 agcaaaagtt cgatcagcag ataccctgtg acatcttgtg ctgatgcttt ttcgatcaaa
    35041 tgaattattt tttgtttgaa ccagacaagc aaaaagccga tcaaagctcc cccaatacta
    35101 gaaactacta gactcatccc cgcgtttact gctgaaaaag atccggtgat cagcgcacca
    35161 agagcaaact ggaaggcagt aacaccagat gcatcattca aaagaccttc tccttccaaa
    35221 atatgcatcg cctttttagg gatattcact cgtccagaaa gagaactgac tgctaccgca
    35281 tctgtcggac ctaacgctgc tccaaaagca aagcaggcag caagtgggac acttggcaac
    35341 aggaatgata acgtcgcacc taccccgaca agggtcaaaa tgactcctcc aaatgccaag
    35401 aacaaaattg tcccgaaatt tttgagaatc gaagaaatat ctgctttttc tccttctcta
    35461 aataacaatg gtgcaataat cataactaag aacaattcag gttcaaaatc gattgattgt
    35521 ccccactctg tcagacctaa taccactcca aggaaaattt ggatcagtgg agctggaatc
    35581 attggcaaaa tgcggctagc tatgttcgat aaggtgatcg ttactgcaaa tacaataact
    35641 aactcagcaa attccatcgt ccatcccctt tattttttat tttgtgtaag catttttaaa
    35701 aattgttctt ttttctttaa aattttggtt tcgactactt caacctgtgt ttggttcccc
    35761 tctaattcac actgtacacg ttttgactcc aaacgaagca gatcatgttt cagacgttcc
    35821 aactgacgat acatcaatat atttctttta cgcaacttac aaagtatttg acggcggtca
    35881 tccatcgaaa gggcgccata gcgatcatgc aacagttttt tttgttgttc aaaactctct
    35941 ctcattttcc agatctccta tacacaaact tccatcatta tagcaaagtt ctttttggat
    36001 gaaaaatatt tcattgtttc atcgtttatt gctagttaga aatttccata aatttttcaa
    36061 cgcctcattc tattccaaat agtatcagat atcaagtaaa acttaagaaa gataaaagtt
    36121 tctgacaaaa ggatgtttac tttcccattc ctgcctttct tgttctttta tcacaacaaa
    36181 aaaatggggc tgcgacaaaa atcttgtcac agtcccttat tctgaataaa cggtgtggaa
    36241 gagacagcct atcagtagta ataagacaca tctctttcac agcctcattt ttgcgtaaaa
    36301 atcgatttat tcgaaaaatt ccccgtatac atcttctaca cgttgatctg gattgatcac
    36361 gatatacaag ttacctgttt tttcgctgat ttttgttgat tgatattgat tgtctaatcc
    36421 ttctgttcct ttatctagat atgggaagta cacaagatca ggtgtgccat ttcggtaaag
    36481 gatttccatt cgctcgatat cttcgtattt caatgcgcga ttaaacatgc ccatttccag
    36541 tccgcctagg ttgatatcat gtgtcgcaac tgcgtcccct tcatggaaga tttcgatttt
    36601 aaatccagca cacggatgga tttcaacaaa ttcactgcca ttgattcgtc caaaacttgt
    36661 tgtgatttgt ttgatccaaa gatcgccgat atgacggcgg ttgatcgtcc aggtttctcc
    36721 atttctaaaa taaaatttta gcgctgtcat ttctttactc attaaaaaga ctcctttatg
    36781 atgtaattta ttcttgtact acctcagttt agcacaaatt tatgtaagtg gtcacatctt
    36841 atgttttttc ttctaatgac gtatctgccc actttaagta gccaacaaag ctgagcagat
    36901 acagtctagt gttttttaaa tcaatttttc tttcgataaa aaatccaaaa cctgtttttt
    36961 atcatttttt attctttgtt ctacgacggc tttttcagct aattgcaaag cttcactaac
    37021 ccacttacca ggtttttttc cagtttcttc tagcaagatt ttaccatcta ctgctaattc
    37081 gcttaatgac ttgataggta aacgatcata ggattctcct acggcatcca tctgaggtgt
    37141 ttcgtcaaaa tagatcatca attcttctac ttgcagtgca tgttttttgc ctaagaagta
    37201 caattcgaat ggttccaaag ctccagtttc tcgttgtcgt agtccttgga caagtgcttg
    37261 aacgtcttta atcatttggt tagaacactt ccacgatttc aaaaaggaac gaatctcatt
    37321 ttcttttaga tccagttggc tgatcaataa tgcccatgct tggctctctt ctttcaatgg
    37381 gacggatggt aattcagcaa aacgtaaaag tgcttcgcct tcttctttca aaccgggaca
    37441 gcaaatgtag cactctgttt caataaatgc atggattccc gcggcccgtt catttcccaa
    37501 gagcatcttg atgaattcca catttatacg ttctacagat attttaccta atagctcgtg
    37561 gttttctttg atggcgtaga aggtttcttc ctctaatttg aaccctagtt gactgacaaa
    37621 acgcaacccg cgcatcattc gtaaggcatc ttcatggaac cgctcatgag gattgcctac
    37681 agctcgcaag atctgttctt ctagatcttg cagaccatcg aacaaatcga tgatttctcc
    37741 atcttctttc aaagcaaacg cattgatcgt aaaatctctt cttttcaaat cttctgccaa
    37801 cgaacgaacg aattcgacat gatctggtct acggtagtcc tgataagttg attccgtccg
    37861 aaatgtggtg atctcatatt gttcatcatt ttctaatacc aacacagttc catgttcgat
    37921 cccaatatct atcgtttttg gaaaaagctg tttgatttct tctggaaatg cgcttgttgc
    37981 gatatccaca tcatggattg gctggttcag caagatatca cgaacacttc cacctacaaa
    38041 ataagcttca tatcctgcat tctctaattt tttcagaact ggaattgcct tttggtattc
    38101 cagaggtaat tgttttaatt tcatttttcc aacctttcct gcgtgtttct tgtcctgaga
    38161 taaagactca gttgcatggt gactgtttga caagtccgtt tcagagtctt caaggccagg
    38221 accacttcat cttgtttact cgtccttacg ctcaaagcga ttatggtctc tttgattgaa
    38281 tttttccatg tttttttcaa aaacttcagt taagtcgata ttcaatgaat tagccatgat
    38341 catcgtaacg aataaaacat caccaagttc ttctgctacc gagttaactg gttcagaagc
    38401 ctttttgctt ttttctccat aagtgtggtt gacttctctt gccaattcac ctacttcttc
    38461 agttaatcga gccatctgag ctaatggaga gaaatacccc accttgaatt gctggatata
    38521 ttcatccact tcctgctgca tactgtataa agaacgcttt ttatccatct ttttccctcc
    38581 aaaactatct taccaaaact ttaacgcaaa tcctagataa gagcaattga gaaaatcgaa
    38641 tttccatgct acatttagga aggaatttct aaatggaaat tctgtgaaaa tacataattg
    38701 aaagctggaa ttcatatggt caaacgaaaa acagtaaaag aagttctttt tatcattata
    38761 ggaacaagta tttatgcatt tggtcttgtt tacttgaaca tcgctaatca tttagcagaa
    38821 gggggagtct cagggatcac cttgatctta agagctttat ttggtattga tccagcttat
    38881 acgactttat taatcaacat cccattgatt ttactaggag gaaagatcct cggtaaacgt
    38941 tccttggcct atactgttct aggtacactt tctttgtcat tctttttatg gatctggcaa
    39001 agggttccac tagaaatcaa tttacagcat gatctactga tcgtttctct acttgcgggt
    39061 ttagttgcag gtgtaggaag cgggatcgtc taccgcatgg gtggaacaac aggcggcagt
    39121 gatattatcg ctcggatttt agaacaaaat tacggcatca ctatggggcg ttccctattg
    39181 gcatttgata tcatcgtctt acttgcttca ttaacctata tcgacttgaa acgtatgatg
    39241 tatacgttga ttgtttcgta tgttttcagt cgtgtgattg attcgatctt agatggcgga
    39301 tattctgcaa aaggtctact tgtcgtctcc aacaaaagcg aagagattgc accactactg
    39361 atgaccggtc tgcaaagagg tgtgactttc ctttctggag aaggcgcatt ctctggtaat
    39421 tcaaaaaaaa tcatctatat ggtcgttagc tctagtgaat taactgaagc taagaggatc
    39481 gtccacgaga ttgacgaaaa agctttcctt tcaatcatca atgtgcatga agtggaaggg
    39541 caaggcttta cttatttgaa gccacagaca agattactga aaaaactata agaaaaaaga
    39601 caaagatagg ctatgaggaa agattccaca tccagctgac ctttgtcttt tttagtacta
    39661 tactattttt aactaattgt tatgttcact tacccggtta cttttctttt gtgtctgcaa
    39721 aaggatcttt cggttaactt catcagataa tttcaaaaaa gcatccctat ctccatcttc
    39781 tagtgcttga tcgatttgag cataaagcat ttttagtttt gcctcttctt ctttttcttg
    39841 aaaatatcgt tcaattaatt cagtatcttc ttcactgact gaagaatccc aagaagaaaa
    39901 tggattgtct tctaagatag atagatattc tcttgtctgc caagcatttt cgaatataca
    39961 ttccacatat aaaggctttt tccaattcaa acggatttca tgaaaaatct ggtctgtgtc
    40021 cgtaaattct tttccctgca aaaacaattt catgggttcc ccttcaacag atagatcact
    40081 gatctgcagt cctcgatccg caaaattcgc cccttcaaca aagtggacat tgcttaaaat
    40141 cgcttcatga ttggcaagat aattcaagat ccaaaaaact tctcttcggc taaaagatac
    40201 atgattgacc atccacgtta aaaaattctt cttttcccgt acatcaatta gcatttttct
    40261 cacctctctg ttaggtcatc taataatgac agtgcctcca tatctcctgg ttcaagtgtt
    40321 aaataataag tcaataattc ttgcgctctt ttcagctgcc cttcttctct taagaaaaag
    40381 gcatactctt tcaagaaatc tggttcatgt gatagttcat gataagcctg ttcatagtgt
    40441 acagctgcaa cggtaaagtc ttctaattca ttataagcat gggctaggtt ccattctgca
    40501 taaggattag cagtttcttc catctgattg atcacttgta tcacatcttc atatctttca
    40561 tcatccaagt aaagattact taaagtaagt aatgtttcat cttgtttgtc tcctacttct
    40621 aaagctttca gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa agcattttcc
    40681 gaggccaggt gataaagttc gacttggtaa ggattttctt tgattccttc ttctaacact
    40741 gtttgtgctt cttcaatcat ttcttcttct tgaagagctt gtcctagata tagatataac
    40801 gattgataat gcggattaac ttctcgaagt tcttccagat agttgattgc ctgctgattt
    40861 tctttcaatt gaagattcgt aaaagctagc tggaacagtc gatcatctgt cctttcttct
    40921 ttcagtgctt cttctaaagg tacgatagct gcttcgaatt ctccttgcat acttaaggat
    40981 tgtcctaaac gttcctgcat cgagattcct gaaatttcat ttatcccgct tgccaacaat
    41041 gtttcataaa ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa ttcagctagc
    41101 gcaaactgga tcaatggttc atcaggaagc aacccagctg cttctttcaa ttttgcttca
    41161 ctcacttcag ggattccgat aacttggtac aaatcagcta tcgccaataa gctttgcgga
    41221 tagtaatcac tttgcttatc cactttttcc aggtattcaa aagctaagtc gatctcatta
    41281 tcttcgattg caatttctgc taacgggatg ttcagtccgt cattttctgg gtattggttg
    41341 atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc caatagccag caattcttcg
    41401 cctagttcag ctaatatatc tgctgggtct tcttttaatg ctttttctaa aaagatttgt
    41461 gcttgtgaca agtcttcatc tgataaagct tgcaacattt tttcgctatt tgtattcatt
    41521 tcaattccct cttatctcac tgacgatttg ttgaaatacc gtcattgttc tcaataattc
    41581 tttttcgttt ggatgttggt atccgtctat tatacctatt ttttctaaaa caagtatttt
    41641 tttgcttttt tctgccaaaa aaagaacttt ttccaaatac tccatctttg aaatctgttc
    41701 cagccagtgg attggatagc caagcaagtc cagccacaca aaaaagtttt ttagttggaa
    41761 atcaaaagga tatgcttgga gattccacac gaaatgaaac atcattccta aaaatttttt
    41821 catactagca gaaagtagat ggccattttc ggtcagataa aaagcttgtt caaacagttt
    41881 accataattt gacaggtttt ctgcctctaa ttgataaaaa tagatcattt cttcgatcat
    41941 cccggcaaac ggccgagtcg ttaattgctt tttatttgga tagtttttat acagattttg
    42001 caaaaaacga tgatcacaaa tcattccgca acagataaaa gtcaaaaagt caatctgctt
    42061 tccgtccacc tgccgctcgc tgattgtttg atcataaacg atgcgttctg gcagattgat
    42121 ggtttgcagc actacacgat ttggtagctg caaaatcgtc cgctgcggtg ttaatgctcg
    42181 gcttagagaa cggatcgata cagggatcat ccatagtgat gaatcaaggg ttgtatgttt
    42241 ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca aatccgatga taaacatgga
    42301 ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc tttaattctg ttaaatgatt
    42361 gcattgagtg ttggcacaaa tataccagtc attgtttgct tgatttgcaa ataacgggtt
    42421 gatcttatct gcaaacaaat cataatagcg ctgattcgtt aaaaataaga ttggctgatc
    42481 aatggaaagt tctcttagct gactgactag cgtttctccg taaaacactt tcgtcaacgc
    42541 atcttctact tgataatcaa gttccatacg catccctcct cttcttattt ttattttacc
    42601 attagtaagc aaattctaga aaagataaga gcaaagctca cctcataact tgcaaataaa
    42661 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
    42721 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
    42781 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
    42841 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
    42901 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
    42961 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
    43021 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
    43081 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
    43141 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
    43201 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
    43261 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
    43321 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
    43381 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
    43441 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
    43501 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
    43561 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
    43621 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
    43681 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
    43741 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
    43801 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
    43861 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
    43921 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
    43981 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
    44041 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
    44101 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
    44161 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
    44221 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
    44281 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
    44341 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
    44401 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
    44461 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
    44521 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
    44581 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
    44641 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
    44701 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
    44761 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaa
//
LOCUS       DAZKMK010000012        40488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000012 DAZKMK010000000
VERSION     DAZKMK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.012"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..959)
                     /locus_tag="ACG8V1_000703"
     CDS             complement(<1..959)
                     /locus_tag="ACG8V1_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0328820.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSP"
     gene            complement(970..1638)
                     /locus_tag="ACG8V1_000704"
     CDS             complement(970..1638)
                     /locus_tag="ACG8V1_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0328821.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1768..2352)
                     /locus_tag="ACG8V1_000705"
     CDS             complement(1768..2352)
                     /locus_tag="ACG8V1_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0328822.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2683..2916)
                     /locus_tag="ACG8V1_000706"
     CDS             complement(2683..2916)
                     /locus_tag="ACG8V1_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0328823.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2931..3881)
                     /locus_tag="ACG8V1_000707"
     CDS             complement(2931..3881)
                     /locus_tag="ACG8V1_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0328824.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3878..4720)
                     /locus_tag="ACG8V1_000708"
     CDS             complement(3878..4720)
                     /locus_tag="ACG8V1_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0328825.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4722..5441)
                     /locus_tag="ACG8V1_000709"
     CDS             complement(4722..5441)
                     /locus_tag="ACG8V1_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328826.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            5989..6279
                     /locus_tag="ACG8V1_000710"
     CDS             5989..6279
                     /locus_tag="ACG8V1_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0328827.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            6351..7577
                     /locus_tag="ACG8V1_000711"
     CDS             6351..7577
                     /locus_tag="ACG8V1_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0328828.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(7665..8057)
                     /gene="rpsI"
                     /locus_tag="ACG8V1_000712"
     CDS             complement(7665..8057)
                     /gene="rpsI"
                     /locus_tag="ACG8V1_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0328829.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8073..8501)
                     /gene="rplM"
                     /locus_tag="ACG8V1_000713"
     CDS             complement(8073..8501)
                     /gene="rplM"
                     /locus_tag="ACG8V1_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0328830.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9035..9502)
                     /locus_tag="ACG8V1_000714"
     CDS             complement(9035..9502)
                     /locus_tag="ACG8V1_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0328831.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9622..11160)
                     /locus_tag="ACG8V1_000715"
     CDS             complement(9622..11160)
                     /locus_tag="ACG8V1_000715"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0328832.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11240..11926
                     /locus_tag="ACG8V1_000716"
     CDS             11240..11926
                     /locus_tag="ACG8V1_000716"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0328833.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12127..12654)
                     /gene="msrA"
                     /locus_tag="ACG8V1_000717"
     CDS             complement(12127..12654)
                     /gene="msrA"
                     /locus_tag="ACG8V1_000717"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0328834.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12738..13415)
                     /gene="pgmB"
                     /locus_tag="ACG8V1_000718"
     CDS             complement(12738..13415)
                     /gene="pgmB"
                     /locus_tag="ACG8V1_000718"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0328835.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13412..14542)
                     /locus_tag="ACG8V1_000719"
     CDS             complement(13412..14542)
                     /locus_tag="ACG8V1_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0328836.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14542..16725)
                     /locus_tag="ACG8V1_000720"
     CDS             complement(14542..16725)
                     /locus_tag="ACG8V1_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0328837.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(16722..17747)
                     /locus_tag="ACG8V1_000721"
     CDS             complement(16722..17747)
                     /locus_tag="ACG8V1_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0328838.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(17792..18619)
                     /locus_tag="ACG8V1_000722"
     CDS             complement(17792..18619)
                     /locus_tag="ACG8V1_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0328839.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHVHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18616..19683)
                     /locus_tag="ACG8V1_000723"
     CDS             complement(18616..19683)
                     /locus_tag="ACG8V1_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0328840.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(19709..20545)
                     /locus_tag="ACG8V1_000724"
     CDS             complement(19709..20545)
                     /locus_tag="ACG8V1_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0328841.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20542..21411)
                     /locus_tag="ACG8V1_000725"
     CDS             complement(20542..21411)
                     /locus_tag="ACG8V1_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0328842.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21481..22764)
                     /locus_tag="ACG8V1_000726"
     CDS             complement(21481..22764)
                     /locus_tag="ACG8V1_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0328843.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(22778..24463)
                     /locus_tag="ACG8V1_000727"
     CDS             complement(22778..24463)
                     /locus_tag="ACG8V1_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0328844.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            24738..25763
                     /locus_tag="ACG8V1_000728"
     CDS             24738..25763
                     /locus_tag="ACG8V1_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0328845.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26358..30011)
                     /gene="rpoC"
                     /locus_tag="ACG8V1_000729"
     CDS             complement(26358..30011)
                     /gene="rpoC"
                     /locus_tag="ACG8V1_000729"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0328846.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(30090..33707)
                     /gene="rpoB"
                     /locus_tag="ACG8V1_000730"
     CDS             complement(30090..33707)
                     /gene="rpoB"
                     /locus_tag="ACG8V1_000730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0328847.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34435..35403
                     /locus_tag="ACG8V1_000731"
     CDS             34435..35403
                     /locus_tag="ACG8V1_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0328848.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35421..36326
                     /locus_tag="ACG8V1_000732"
     CDS             35421..36326
                     /locus_tag="ACG8V1_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328849.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            36323..37129
                     /locus_tag="ACG8V1_000733"
     CDS             36323..37129
                     /locus_tag="ACG8V1_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328850.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37286..38254
                     /locus_tag="ACG8V1_000734"
     CDS             37286..38254
                     /locus_tag="ACG8V1_000734"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0328851.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38571..39095)
                     /locus_tag="ACG8V1_000735"
     CDS             complement(38571..39095)
                     /locus_tag="ACG8V1_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0328852.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39382..>40488
                     /gene="scm"
                     /locus_tag="ACG8V1_000736"
     CDS             39382..>40488
                     /gene="scm"
                     /locus_tag="ACG8V1_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0328853.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSD"
BASE COUNT        11903 a         8374 c         7213 g        12998 t
ORIGIN      
        1 ggcgatgaca aatttttttt attttcgatt atctttcctt tggtagggcg aataatcttc
       61 atgagtattc ttagtaaggt tgttttcccc acaccgtttt tccctaccaa accaataaca
      121 tctccaactt tgacatcaaa atttatgtct tttagaatat tttcataagt aatttcttca
      181 acttttaata catctgcact taccttttcg atatagggaa cttcaatcga actattaaaa
      241 atgtcaaatt tccttaatgc gttattgttt tcagttttta acaactgaat agttgtagtt
      301 tttacagttc tatttttaac aaatagaact tgatcaatta attctttcaa atagtaaaaa
      361 cgatgttcag ctataacaat agtaaatcct ttatttttta aatcacaaag taagtcagca
      421 agtttcttag tcgctttgaa gtccaaattt gaagacggtt catccaatat aattaattta
      481 gagtttagcg ataaactaca tccaatagaa attttctgcc gttcaccact tgatagcgaa
      541 aaaatgtttt tatctaatat atttgaaaga tctaataatt cagctaattc attgattctt
      601 ttatccatat gctcttttgt aaatccaaag ttttccatcg aaaatactaa ctcactcgtt
      661 gtatttgttg taaagaattg tcctctagga tcttgactta cgtagccaat attttttgat
      721 atactttctt gtgttaagga ttgtagcgag ttattttcaa ataaaatttc cccaaccaat
      781 tttccgtcat aaagtcttgg aataagtcca ttcattaact ttaatagtgt ggatttccca
      841 ctcccagaag aaccagtaat caatgtaaga ctgccttttt ttattgaaaa attaaggtca
      901 acaagcgctt gttccttaac atccttatat gaaaaagaaa cattgttaaa ttcaaacaat
      961 tctttcacct caaatcaaaa tacttaagaa aaaaatagta acggaaaaca ggatgatata
     1021 gtctaataag ctcatgtttg ttttatagta agaacttctt ttcacaggag attctatccc
     1081 ctttgttatt gcattagcgg tcatttcttc agcaatcata gttgcacgta taagaacggg
     1141 aactaacaga aattccaaat attgcagagg ctgaaaaaaa gacaagcgaa tgtttctcat
     1201 ttttaaagat tctttaatca ttcttatttc agaacgataa gtcggaaaga atctgatcac
     1261 tactgtaata cttaacatta tttttttcgg taaatgtatt ttttctaaag aggataataa
     1321 ttcattgcta gtatagacac taaccaacaa ccatgagatc attacgaccg gcaacattcg
     1381 aaatacaata tataaggatg tcgcaaaaaa tatagtaaaa aactctatct tgatagcatc
     1441 aataattttc attactaaaa acagaactga ataaatcaag atgaaataaa tacatgtctt
     1501 tattttacga aacatcaata ctaaaactgt agcaataatg aaacacccca cttgaaatat
     1561 atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata tttaaagtaa tcaaggttaa
     1621 cggattgatt tttgacactc tttcacctcc aaaaaataag atcgcactgt tatcttaagc
     1681 tgataaaaat gcaccgttat tcagataaca gtgcgatctt atcaaaaagc ataactgaga
     1741 acaattctca atgcaagctt tttttaatta actcagtaat tcaatccacc caggaacaga
     1801 aaattgatac aatcttctgg ttaatagcat agtcttttct tttgaatagc tatgcttcaa
     1861 tatatcatag aatgtagcgt agaatgagta aatgtagaag tggatttctt cttcattaaa
     1921 gactaaattt tgattgatca ttccgatagt ctttaactta tctagacaac agactgtctt
     1981 tttcgtcaca aatgatgtaa ttttttcaac aatatcttga tatttggttc cagctgattg
     2041 gaataaaatc aaatcgaata tacgtttatt ctcaaaaata taattaataa atggatctaa
     2101 ttcattgaag ttctctctcc aacttttctc tatatctata ttttctaagt ttaagaggta
     2161 tttggtaaac tcttcattat acatattaat aagcgcatta atatgcggct gaataatttc
     2221 ttcaaataac gcttccttag aagtaaaatg tttataaaaa gcacccgtag ttacgcctgc
     2281 ggtcaggcac agttgcctta tcttagtttg ctgaaatcct tctttttcaa aatggcttag
     2341 tccactttct attaactttt ggtgagttat aatataatct ctggtttcta tgggtacttc
     2401 ctcctaataa gatgaattaa ctttattatc ttacacagga cttgaagttt caatcgttaa
     2461 cgtttattac tgaactcatt ccttagagct aaactaatga gaaagagcag aataactgat
     2521 acaaaaagca atcctacaag aattaataga ctcatctaaa tcattcacct tttctaaaat
     2581 aatccgagaa agatttaaaa aaattatatc cagctattag gataagtcct atcataatca
     2641 ctccaatagc cattttctta tcctccctaa aaataaagtc ttttacttaa aacctttttt
     2701 gtaatcgtca ataaattttt tccctttatc agttaattga aatcctatat ctaaatagtc
     2761 ctcatatcta agtttagcca gcatctcact tacagatgct ggactaacac ttagaacttt
     2821 tgctaaatca ttatttttta atttcttttt tctttttgat aattcctgaa ttgctagtaa
     2881 ataaattagt atagtagtcg ttttaatttg taccatacct accctccaaa ttatttcgaa
     2941 aggccttcag caattttatc caggttccat ttcatcatcg catagtaact atcaccatct
     3001 tgtccttttt ctgcaattga atcagtaaaa atcgttgaat agataggaat attggtatct
     3061 tttgatactg ttttcattgg tctatcatcc acactgcttt ctacgaataa ggagggaact
     3121 tttgttgtgc gtaatttttc aactaagtgt tttatttgat ctggtgttcc ttcttcttca
     3181 gtgttgattt cccaaatgta agcagaaggc acattatacg ctttcgagaa atatttaaag
     3241 catccttcac ttgtaacaat cattttttta tcattcggaa ttgaagcaaa tttagattta
     3301 gcttctttgt ctagagaatc caatttttca atatacttat ctagattttc tttatagaat
     3361 tttttattat caggatcttt ttccgctaac tgtttttcaa tatttttagc gtaaataata
     3421 ccgttttcta aatttaacca agcatgagga tcttccttcc ctttctcact ctgaccctct
     3481 aagtaaataa catctatgcc atcacttgct gcaaaatagt ctttgttttc ctctttgttc
     3541 gcatttttaa ctaattttgt aaaccaagca tttcctccag tttccaagtt aacaccgtta
     3601 taaaaaatca aatctgcttt tgaagttttt tgaacatctt caggcaaagg ttcatattcg
     3661 tggggatctt tcccaatagg tacgatactg tgtaaatcaa ttttatcttt tgctatattt
     3721 tcggtaatat ctgctaaaat cgaattagta actactactt ttaatttttc gttcgaagcc
     3781 actttatctt tgtctttact atttattgaa catgccccga atataaaaag agcaatcaaa
     3841 atccccagac ttcccagtac tgctttccat tttctcatca taacgatctc tccttctttc
     3901 ttaaaaattt ttgttttggt gagattagaa aactaattac aaataagaat gccgctgtta
     3961 aaacaatact tgatcctgct gctatgttaa aactataacc aataaacaat ccaagcaatg
     4021 atgaacttgc tccgactgtt gcagaaataa atatcatagt tttcaagcta tttgcatata
     4081 aatatgctgt agctgctggt gtaattaaca ttgcaacaat taatatagtt cccacacttt
     4141 gcatagctgt tactgctact aaagttaata gaaccatcaa tagataatga taaaaactga
     4201 cattcatccc tattgctttt gccattaatg gatcaaaaga agtgattaat aattctttaa
     4261 aaaatagaac tactataacc aacactaaaa ccgaaacgcc aattgtaatc cacttatcaa
     4321 tatcttgtac agctaaaata tttccaaata aaatatgaaa taaatccgtt gagctatttg
     4381 caactcctat aaggataact cctagtgcta aaaaagaact gaaagtaata ccaattgctg
     4441 tatctccttt tattacacta ttgcttttta taaaagtaat aatgatagaa gctaatagac
     4501 caaatacaat cgcaccaata aaaaaattaa tacctaaaat ataagaaatt gctactcctg
     4561 gcaaaacagc atgagaaata gcatcaccca taagagacat tcctcttaaa ataataaaac
     4621 aacctactgt tccagaaaca atccctataa ctacagatgt aattagagca ttctgtaaaa
     4681 agtgaaagtc atacagacca ttaataaaat tttgaagcat attattcccc ctctccaata
     4741 aatatagtat ctccataggc tttttttaaa ttttcttcat taaatacaga ttctacactt
     4801 ccgtgagcaa ttagatttcg attcaaaaga ataatattat caaaatactt ctttaccttg
     4861 cttaaatcat gatgcacaat tagcactgtt tttccctgat gtttcaattt ttttaacgta
     4921 tccataatta ttcgttcact aactaagtct atccctacaa atggttcatc taaaaatatg
     4981 aaatccgcat tttgagcaag acatcttgct aaaagtacac gttgaaattg tcctccagat
     5041 aattctccta tttgatgatt agaatatttt cccatatcga ccttttccaa agcctttgaa
     5101 actctttgcc attctgcatt tttaattctt tgaaaaactt tcattccggc atatgttccc
     5161 atagatacac attcttttac ttttattgga aaagtaaaat caatatcagc tttttgctct
     5221 acatatgcaa tcttcaccag ttttttattg atatcttcat tattaatgga gacctctccc
     5281 tgatgaggga taatatttaa cattgcttta agcaatgtgg attttccagc accatttgga
     5341 ccaataatac ctgttaaagc accttcttgt atttttaaag aaatctcgtt caatgctaaa
     5401 aaatcgttat aacaaacatt aagctttttc acttctaaca taactttctc cttctaaatg
     5461 ataatgatta tcacgatcat atggtataca aaaattatat tttagtcaac cctaaatttt
     5521 atagtttaga tttttttctg ttgctgaaga ttagaatgac aaaactattt gaaactacgc
     5581 gtatagtaag tagctttcaa aatatataaa aaaggagcgt tctcaaatga gaacatgctc
     5641 ctaaaactac tttattggcg tatggaaaaa ttatacttga agtggatagc gggttgaata
     5701 agttgataag catcaaaaca tttataaaaa ttattcaaga ctatgtctgt tccatataca
     5761 aatatgaaaa tcagtaaact ccatttaact tttttcgtag tgtgtctttt tatacatgtt
     5821 tatcgttatt aatagtgaat aggaaaaaaa ctatttttga tcattgacaa ttaaatacaa
     5881 tttttgaata acgatccctg ctattcgagc tagaaactca agtacgccat gacctgatta
     5941 gcgacaaata cttagagatt tctttttgtt ctgggaaaac aaaatgccat gacaatagca
     6001 gcaaataatg atacttccgt aaggctcgcc gtaaactggg gagaggtgaa attcctatgt
     6061 gcttacccaa agcaattcaa aaatttcata actactattt taggagatga ttttatagaa
     6121 tccagagtct acctgaataa aaaacaaacc tgtgcatttc taggaatcga taataatact
     6181 ctagataagt ggattcgtat atataatttc ccagtaataa gagttggtgg tgtagttcgc
     6241 tttgacgtag aagatattaa agactttatg aatacttgaa attttcttct cgccgcatat
     6301 actaaaatta gtgaaatata ctgccttact ttttttaagg agtgaggatt ttgcctacaa
     6361 agctttcaaa tgggaaatat aaaactaatc tgagatatcc aaaaaggttt aaagaaataa
     6421 caggaattgc atcagaaaag tatcaaaaaa catttccaaa taggcaattg gcgattaaag
     6481 ccgaaaatga tatgaagaag aaaatagaaa aagttttgag agaggagaat gcaaactcat
     6541 tagaattaaa aggaaaaata acgtttaaaa aattttacga atctaaatgg cttcctagat
     6601 atgaattagg tcaaacaatt agatcaaaca ggcctcctag tgatataacc attagtaata
     6661 caaaagatat atttcggttg catattttac ctatgtttgg tgaatacgca atgaattatc
     6721 taaatatcaa tactgagata atatcagatg aattaaccaa aaaatcgaaa gaatatgcaa
     6781 atataaaaat cataaaagga tatgttcgtt ccatgttcga tattgcagaa atactaaatt
     6841 atatcgaatt taatcgaact actaaaatta ttcaaagtat cactgcacct aaaaaaaatg
     6901 ctttagaaga aaaaagaata caagaaggta agcaagcact atcatcaaaa gaacttacta
     6961 attggataga agcagtaaat gatgatttta ataatcattt acttactttt catgattaca
     7021 cactttttat gattaccctg tatcttggtg atagaaaaag tgagacttac gctttgcaat
     7081 ggaaatatat tgattttgaa aaacaaacgg tccgtcttaa acatacttta gataaatatc
     7141 aaagaaaaaa gtttacaaaa ggtcgaaaag ataccgtcat tcaagtacca gaagttgtaa
     7201 tgacattgct aagtgaatgg aaatcggtac aagctgatca attgttgaaa ttaaaaatta
     7261 agcaaacgtt agatcagtat ctctttactt atactaaacc atctggagaa gtaaattgtc
     7321 ctgttcatgc cgattaccta aactatcgaa tcaattcaat taaaagaagg catcctgact
     7381 tagttcattt atctccacac aaactccgtc atacttatgc aactatagct agacaaggtg
     7441 gagcagatat gaatcaaatt tcaaatgctt taacacactc agatatttct acaacaaaga
     7501 tatatgtgaa cacaccagat atagttgata aggctgtttt tgaagctttt cagagaggat
     7561 tgaataagtg tgattgatat gtgttcttaa atgtgttctt aaaaacaaaa gaagcacttt
     7621 ccaaataatt gaaaagtgcc agaaacgttg atatataaag gatattaacg ttttgagaat
     7681 tgtgaagctt tacgtgcttt cttaagacct ggttttttac gttcgaccat acgtgcgtca
     7741 cgagtaagta agccagcacg tttaagagct aaacggaagt caggatcaac ttctagcaat
     7801 gcacgagcga taccatgacg gatagctcct gattgtccag cgtatcctcc gccgtttacg
     7861 ttaacaaata cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg attgattact
     7921 tcacgcaagt cagcatgtgg aatgtattct tcaacatctt ttttatttac agtaattttg
     7981 ccagttcctg gtactagacg tacgcgagca actgcgtttt tacgacggcc tgtgccgata
     8041 tattgaactt gtgccaatga aattccctcc tattagatta agtttgtgat gtctaaaact
     8101 tctggttgtt gtgctgcatg tggatgttct gctccaccgt atacgttcaa ttttgtaaat
     8161 tgtttgcggc ctaaagtgtt tttaggaagc atacctttta cagaagtttc gatcaaacga
     8221 cgagaatttt tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc tgggtagttg
     8281 ctgtgacggt aataaatttt gtcagtcgct tttttacctg ttaatttcac tttgtctgca
     8341 ttgatgacga tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg tttattttta
     8401 ccacgtagta cagatgcaac aactgttgaa agacgtccca atggaacatc agttgcgtct
     8461 actacatacc atttacgttc tacttcgcct ggtttggcca tatatgttgt acgcacgtta
     8521 ttttcctcca atttaagttc tgatgtttga catacacttc gagtttccgg ggctctttgt
     8581 ggggcaaaca ataccattta acatattacc cgctaatcta gtcaatgtca acgtttttct
     8641 gcttgattga aaaatttata tgaaacttta cttcttttct ttcttttata taaaagggtc
     8701 gtttcctgtc aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc acaacccctt
     8761 attttgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccataaa
     8821 acataagaag ctgtttttta aaattgtttc ttgtgactcg gagctgaacg gcaggcttac
     8881 aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
     8941 aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatttca
     9001 caacctcttt ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct ggcgctttgc
     9061 ccaaatgagc ttggatcatc cagatacgtt tttctgtggc tgttttgaat tcgataaaga
     9121 tatcttgtgt tggatcgtcg ccttcttctc cagaaacttc gattcctttt tggtaaagat
     9181 ctgctagata acgatagcct gcaacaagtg tttccaaacg ttctggaata gtacgatccc
     9241 agcggccaat ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat gtagaataag
     9301 gtgaaccgtc taatgtgatc aagcgttcgg atacttcatc taactgagcg ttcaaatcat
     9361 ccatgaactc atccatcatt gggtgcaatg taaggaaacc tggtcctctc atgtaccaat
     9421 gtgtttggtg aacaacaaca gacatttggc ttagatctgc aactaattga tttaatactt
     9481 ctttggtttg ttggaatttc atctttcttg cctcctaaca ttttcttaca cttaaaataa
     9541 tagtagactt tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat tttcagttcg
     9601 cttttttatc cgtaaagcca atcatttttc agaatcagct aattcttctc gcagttgagc
     9661 aaattgctga taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg gatagatttt
     9721 ctgatatttt ttatgatcag tttcttgtac tccaacttgc tctttcaatt ctatcaattc
     9781 ctgtgcttca ctgatacttt ggatctttcc gatgcttctc cagcctaaaa tcacagctcc
     9841 taatgcggat gcttctgtga cagtagggaa aacaagcggg taacccagta catcagccat
     9901 catctgacga aaaatcggag attgagcaaa cccgcctgta gcacggatct ctgtgagctg
     9961 attttcctgg caaactcctt ttaaaatccg ataaaggttg agacagatcc cttctagaat
    10021 cgccttcatc atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac tgccaaacgc
    10081 atctgcgtcc caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa tcagaccatt
    10141 ggcacccgct ggcacaaaag cgatagccga aagcagtgca tcatatggat tttctccttt
    10201 ttctttagca gattgaacga cttcctgtaa aaatgtatgc cctgcccaat caaagatgcc
    10261 agcaccattg ctagtcgctc ctcccactac ccaatgtttc tgatccagaa cataacaaaa
    10321 cgtctccatt tctggatgga actgaggctg agtagtaata aaacgaagtg ctccgctcgt
    10381 tcctacggtc aaagcggcga tcgtatcacc aaaagcacct aaccctagat tagaaagcgg
    10441 cccgtctgcc cctcccaaaa caaatgatat atcatttggc aaacctagtt ctgctgctgt
    10501 ttttgatggt agacctgtta cttgataggt ggaaggaaag acttgcggca attgctctat
    10561 tcgaatgttc aagtagtcaa gcacttcttc tgcccattgg aattcatgga tatcaaaata
    10621 tccagttcct gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc tataaaagat
    10681 atagtctttg ataccgacaa aataagcagt ttttttgaat aattcaggtt ggctgttttg
    10741 cagccacttg attttgttca atggtgtcat tggatgaatc ggcgtcccag tctgccgata
    10801 aaattctttg ccaagcacgg tatatttcag tttttctgct tcgtccgctg ctcggttatc
    10861 tgcccaagtg atcattcgag ataacggtgt tttctcttca tccaacaata tcaggccatg
    10921 catcgcagag gagaaggaga caaattgaat gattgtgtct gaaggcaact gtttaattac
    10981 ttcttttata acggtgcaga caccttgaaa gatcaattcc tgatcttgtt ctgccatccc
    11041 ttgtgtttct tggatcaaag ggtagcgaaa ataagcggat gctgcttctt gggcttcatc
    11101 tgtgaaagca actgctttgg tttgtgtggt tccgatatct aatccgattg aaacgttcat
    11161 tttttcacct caaattttat gtatgtacaa aaatttagtt taagcttgcg tatcttggtt
    11221 ttgaaaaggg ggagatggcg tgttacttta ttttatcatt ggttgctgta tcggatcatt
    11281 tttatgttta accgcccagc ggattccgtt aggtcactct attatttatc ctagatctca
    11341 ttgtgtcaaa tgctgtcgtt cgctttcttg gtatgaattg attccgattc tctcgatcat
    11401 cgtccaacga tttcgctgcc gctattgcag gtgtcggttg cccttctact atttgttggc
    11461 agaagcctta tgtggtggtc tttttgcctg gttctttact ttttcttccg caagaaattt
    11521 ttcgacgttc atatggatgc tttcggcttt gctattttcg ttgatggatt tattttactt
    11581 catggtccat tctcacacgc tattctgttc ttgggcaatt ttatggattt tttggttgca
    11641 ggccggcgtc ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg gtgtgtgcct
    11701 tcgctatggt caatcctatc tcggagctgg cgatactctg ttgatgcttt cttggagtgg
    11761 tggcttgtct ctagaagagt tgcttcagat cttgtttctt gctagtctgt ttggaatatc
    11821 cgttttttct gtttacttta tctgtcataa aaaaaagttg gaaaagctgc ctttcattcc
    11881 ttttttgagc agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa aggttgtgac
    11941 aaacgacttg tcacaacctc tgatatatag tgttctctta ccaaatcctt gtgacttgga
    12001 gctaggcgac aggttcacaa cctctccact aaatggtgtt caatcagctg accctcagta
    12061 ttagctcaaa atgctggttt tacaacctct gatatacggt gttccataag tagctttttc
    12121 ggcttcttat ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc cagaaccttc
    12181 tcgataaaga ttgtaatgga gcttgttttt cttgtaataa tcctgatggt aatcttctgc
    12241 aggataaaat tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga atcgcccgct
    12301 gtcttgcaat gcttgttttg aacgctctgc aatatttcgt tcttcttcat cattatagta
    12361 gatcactgga cgataagaat caccgcgatc agcgaactgt cctaatgcgt cagtcggatc
    12421 ggtttgctgc caatagattt ccaccaactg atcataagaa ataacttctg gatcatattc
    12481 gatctctact gcttctgtat gccctgttgt gcctgttgtt acttgttcat aagttggatt
    12541 tggtacatgc ccgccagtat aaccggatgt cacagaaatg atccctggct gctcttcaaa
    12601 tggtttgacc atacaccaaa agcatccgcc tgcgaaaact gctttttttg tcatataaaa
    12661 tccccctttt ttctcatcat accttgattt ccaaaaggag gataacgttt tgcttgtgac
    12721 agaaaaaacg gaaactatta tatacgtgat acggcttctg ataaatgcca aaacgaacgt
    12781 acttgatcaa aaacggttaa atcaggaatc acatattcag cagaaggaag atttatgttt
    12841 tcacctattc ccaatacatg catgttacca gccaaagctg cttgacaacc agcttctgaa
    12901 tcttcaatga ccaagcatgc atttggcggt acattcaatt gctgtgctcc tttgagaaaa
    12961 acttctggat caggttttgc ttttgatact tgcgtgccgt caataaggac atcaaaataa
    13021 cttgtcaaat tcgttttttc taaaatgagg cgtgcatttt tgcttgctga tcccaatccg
    13081 atcttgatgt ttgttttttt caagtactca agaacagcta ttgcaccagg caaaacggat
    13141 gtttcatcca ttttttgaat cgcttgaaga tatagattgt ttttttgctc agcaaaggct
    13201 gatttctctt tttctgagaa tttctgttcc atttttccaa atgacagcaa tcgctctagt
    13261 gactcggttc tggatattcc tttcagttgc tcgttttctt tctcagtgaa tggaatggac
    13321 agctggttag ctaaaaccag ccacgcttcg taatgataac gagctgtgtc tacaagaacg
    13381 ccatctaaat cgaatagtgc tgctttgatt ttcatcgatt acctccttga aaatgaacga
    13441 cttgaagtaa agatgcgatg tttttagctg tgcgatgaag attatcagtg gcttgctgta
    13501 aagcttcttg taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa atgccaacat
    13561 ctgctgcttt ttcgcttcct ttaccaacac ttccgcaaat agctatcaca ggaacgccct
    13621 ctggtactct tctagcaatc ccaactggcg cttttccttt tagactttgt ccatctagac
    13681 gtccttcgcc tacgattacg aggtctgctt gctgtacccg tttatcgaac tccagtaaat
    13741 ccaatacgta gtcgatgcca gagactattt tccctgaagc aaatgctaac agtcctgcac
    13801 ccattccacc accagcacta ctgcccgatg cctcgattac ttgaggggca aatctcatat
    13861 aaaaatcatg catggcttgg tcagccactg ctaattcatt ttctttcaag cctttttgtg
    13921 gtccgaaaac aaaagaagcg ccttcttttc cgcataaaag attagtaaca tcagtaatga
    13981 cagtgatttt ggctttaatc agctcaatag gcacgttcgt tttatcaact gtagcaattt
    14041 tacctagatt ttctcccagt gcttctactt tttgattatc ttgatcgtaa aaagcataac
    14101 ctagaccttc tgccatgccg atgccgccat cgttggtact acttccgcca acgccgatga
    14161 tgatgtgtcg aacaccttgt tgcaacaatt gtcgtatcag ttctcctact cctcgcgtgg
    14221 ataacctaag tggctgttgt tggtttagtg ggacatcttt caatccgacg atttctgcca
    14281 tttcaaaaat cgcagtttga ccgtctgaag cgtactggat ttctttgttt ttgccaaatg
    14341 cattagtaac gactgctgtt tgccgcttca accttaaact gtctgctaat gatgaaaccg
    14401 ttccttctcc tccatcgcct ataggtagca gatcatattc agcctgaaca aaaacttctt
    14461 gaaaacctga ttgaatggct tgtgctgcct ctaaggctgt cagactttct ttgaaagaat
    14521 ctggcgcaat cagaatacgc attattttcc cctccgttcc aagatcagtt ctttttccag
    14581 ctggtattgg tctccattga caaaaatagg taatgtttct ttcgtctctt tttttattac
    14641 tatttgtttt tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa aaggaaaaga
    14701 aattttttcc catgctgaag gaagtcttgg agaaatagaa agagttccat tcgttactgt
    14761 aatacctgaa aaaccaaata taactgctag ccatagtgct cctaatgaag cagcatgaat
    14821 tccgtcatct gatgaatgcg gatctggtcc taaatcaatc aggcacgctt cttggaataa
    14881 atgatacgcc cattccattt cttggatacg gcaagcctct atggcgtgaa tcgctttact
    14941 caacgatgag tcgtggattg ttttgttttc atagtaatgc aaattcttag aaacgattgg
    15001 ttgagaaaaa tactcaggta ataagtataa cagcatgaca agatctgctt gcttgctgac
    15061 ttggagttca tttacttcgc tacgggcata atcaagtaaa atagactggc tgccaggacg
    15121 ttctttgtaa gtggtcaaat caataactgg cttactgaga tacgtgtcat cttgcggaat
    15181 cagctgttct ttgttaggca ctggcaaata taacttatct aaaaaatttt gacattcttt
    15241 caaaaaaagc ggatcgccag cttgatattt aacagctaag gccacattat aatgcgccag
    15301 atagttcgta tatgcattat tgtcgatata ttctgtgtat tcatctggac caatgactct
    15361 gtgtatctct aaatgatcat cgacttttac agctctgctc agccaaaaag ctgctgtttc
    15421 atgaatgagc gtttttccct ttgtttctaa aaacgattga tcctctgttg cttggacata
    15481 ctcttgaacg gcataagcaa ggtctgcact gatatgatgt tctgcggcag ctgaagcaac
    15541 ttcttgtctt gttccggttt tgatattcaa tgcagcaaat ttgggtgtct cttcctgacc
    15601 agtgtgtgcg ctttcccatg ggaaaagtgc accttgatag tgataatctc ttgctttttg
    15661 ccgtgcgcct tctaatcgat cataacggta cagcagcaaa ttctttgcta cttcaggaaa
    15721 ctgatataaa aagaaaggca ggataaaaat ttccgtatcc cagaaaacat gccctttata
    15781 accttcgcca gttagtccct tcgcccctac actaacactt ggatcattgc ggggagtcat
    15841 aatctgtaaa tggtaagcag cgaagtccat cgctaactga tcgaaagaag aatcgccttg
    15901 gatcgttatc cgatgctgat gccaaaaatc tgtccaataa gacatcgact gctctaaaat
    15961 ttctgtataa ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg ggtctgtttg
    16021 ctgaagagag gtagagagca cgatgatctt ctccagcgta aaggacgtat tttttctgat
    16081 cttttctgaa aaggaagcgg tgatccggcg attttttgct tggaatacac ctgctttatt
    16141 gaatttagct gcaatcccaa tatcaatttt actttctgtt gtctggtagg atgcactgat
    16201 caaatcgtca ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac caaaattggt
    16261 ttgctgtcca tcgatacccg ttttcacatc tacttggcag tcttcggtca acggcttgat
    16321 gaccactttc agggctgcct gatggagatt attttgatca gcaacacgac aagtaatgac
    16381 ttccactttt tttccttgag cagtttcaat gagcgcatag cgtttaagct ctcccatttc
    16441 taaatctagt gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa aatgttcacc
    16501 gtcgattttt atttcccatc tgatgacatc tgggagattc actaactcgg ctgattctgc
    16561 tcctaaaggt cggttatata caccagctgc aaacattccc cttacttgat tccgatagtc
    16621 ttcctcctgg cttcctctga taccaaaaaa tccatttcca atggtcatct gactcgcata
    16681 cttattgata tactctaaat caaactgttt ttcttctact ttcataaggt aataacttct
    16741 cctgtttctg ccgaacgata taaattttcc accagattct ggatgacata tccttgccag
    16801 ccatctgcaa tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg agccatactt
    16861 agttcatgtg ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt tccatctaca
    16921 tcttcgaata gttcgcctgg atacagatta gcacctgcct gatcgccaca aaaagccaca
    16981 ttcagtttgt cttctctgat attcaaagca aacgaagtat tcagttggat gatcttgccg
    17041 ttttctaatt ccagtgtgcc aaaaattgca tcttctactg tgaatttgct tggatcccat
    17101 ttcccgaatt tccctgaatt ttttttcgta ccaatttttt ggaagctgta cgcccgaacg
    17161 ctttttactt taggaaatcc agtaatatac atcgctgtat caagcatgtg aatgccataa
    17221 tcgatcaaag gcccgcctcc ttgaagttct ttgtcgataa acgttcccca gcctggaaca
    17281 ccagaccgac gaagcgcatt cgcttctacg taataaatat ccccgagaac atcaagatga
    17341 ttcttgatca ttgttgcttc gtcagaaaaa cgatgctgga atccataagc caaaatctgc
    17401 ccgtttttat cggctgtctc tgccatcttt cttgcttgat cagctgtcat agctggtggt
    17461 ttctcgcaca tcactgaagc attagcttcg agggcagcca atacatgttc gaagtgaaag
    17521 cgattgggtg tacagacact cacgatatcg ggagaaatct cattcagcat ttgtgtaact
    17581 gatgtatacg catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg ctggctgact
    17641 ggatcaacca cagcggccaa ttctaagaca tctgatcttt tttgataata agcagcatgt
    17701 actttttctg ctacttgtcc agcgcctatg attgctactt tcttcattta tttctccctc
    17761 ctttgcttat ttacttattg ttattctttc tctattcttt ctctaaatca tttaaatgac
    17821 gcgctgtttc cagacagttt ttcaagtatt gcaatgattc ctgataaatt ttcacttcat
    17881 tttctcctcg caaacgacat tcatatacca tataaccttc atactcattt tgaagtagcg
    17941 tttgaaaatg gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga tgattatctg
    18001 ctaaatggac atgacccaca tactcttttt gcgtagtcaa tgcttttgaa atatcatctt
    18061 cttcaatatt catatgataa aaatcagcga tgatctgcac atgtttcaat tgattagagt
    18121 caatgtaatg acgcgcatcc gctaacaaat tgatcatatg gtcttggtat cggttcaaag
    18181 gctccaaata aataaccgta cctgtttctt cggctacttt atctaaataa cgaagtgatt
    18241 cgctgactgc tttatagtct ccttctttgc ttcggggtga aaccatcggc ggtaatcgat
    18301 aagtgaacat tccccaagct gctgggataa cgattccttt gcctccaacc tcgcttagtg
    18361 cacgcaaaat ctcagcaatt tgacgtacac cattaagacg ccgttcttcg ataaagtcac
    18421 cgatccaccc atcgtagccg ttacaagctg tcactactgg gatccctgtt tctttgattg
    18481 cctgtttgac tgtgtcgata ttttctatta acaacttacc gtcgatttcg tattcttcaa
    18541 atccacagtc tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa aatgcttgat
    18601 cttgcgtacc gaatttcatt gttctcctcc tcggacttga acgcccattt tgatactttc
    18661 ttctgggtgc tggtctacat aacgcatgta agcttctgcg ctttctgcaa atggcacaac
    18721 tggatcaatg atttcttcac aatccaagta accgttcatc aataattccc aacatgtgtc
    18781 ctcgattcgt ttacggttcc atcttgggta atctgggttt ggttcactag atgctcgagc
    18841 aaagacgatt tttgcattat tgaaatgtgc ttcgcgacca agattaaacc cagctggaaa
    18901 aggtttagca aaggcaacat aagaaatgat cccgccatag ccgatccctc ttaaagaagc
    18961 ttgtaatgcg gcagcactgc cactcgtttc aataactgaa tctactccac gtttatctgt
    19021 caattccttt agacgaaaac cgacatctgc tttcatagga tctagcgtgt aatcggcacc
    19081 gtatttttct gctaattctc tacgctgcgc tatgggatcg atcccaacga tggtagtagc
    19141 acctgctttt ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac cgaagattgc
    19201 tacagcatca cctgctcgaa catgtgcttc ccggatacca cctaaagcga attgagcagg
    19261 atcgtaacat actgcatttt tccattgatc caaccgatca agtttacgta gtttataatt
    19321 atcaacaccg tttgtaatca gcgtttcacg aatgggtcca taagtgcaga ctaactctcc
    19381 tttttgatac tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa tcatatttcc
    19441 taattgaaat tcgccaaata ccacaccgcg ttggcttcct tcttctcttg gcaagaatag
    19501 gcgccattct tctgaaaatt cttcatcgat aaatggactt aatcctctaa agtcgacaat
    19561 ttctgttcct ggttttggag tagcgaaaat tactcggata cgtacttcgt tcttttcgat
    19621 ctcacggtct tcgtaatctt tgaatccggc tattcttggt tctaatgcaa ctaatttttt
    19681 catcttttct ttcctctttc ttttcttttt atccttttac gccgccttcg actaaatcac
    19741 ctttaataaa tcgttcagat aacgcataca taatgatgac tggtaaagcg gtcaccaatg
    19801 aagcggccat cattcgtccc caaatgtagt ctggtgtact gaataatgtg ttcaaaccga
    19861 ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa taatcattcc
    19921 acgaaaccat aaagcaataa acgaagacag aaatgattcc agaaatagcc aatggtataa
    19981 tgatccgaat aatgatctgg atccggctaa gaccatcgat cattgctgct tcttcaatat
    20041 cagtcggaat cgtgtcaaaa taacttttta gcatgaatat cgcagtagga agtgtttgaa
    20101 cgaccatcgt gataatcagt gctgtattgg tatcatacaa acctagaccg gaaatgattt
    20161 taaataatgg aacaacgatc aaaataccgg aaaacatata aacggtgtaa aagctagcat
    20221 tgattttcga gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg ctgatcccaa
    20281 tcactactgc agttacggag gctatcaagg agatcactaa actatttttg aaatacatga
    20341 tgaatggaaa aatctcagca ttgaaaatat cctgataatg ttcaaaagtc cattctttcg
    20401 gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat gtcatcagca
    20461 tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg taaaataaga
    20521 ttttcctgct ctttttattt ttcatttcaa gtccaccttc tttcgtgaga gcaagacgac
    20581 cgctgcaata atgatgaaca atagaatcga tatagctgct gcttttccaa gatcattgaa
    20641 cgcaaaggct gtctgataca aataaacgcc taagatattg actttattcg tcagtagata
    20701 gacatctgta aacatataga acatccagat agatcgtaaa acaatcacag ttgctagtac
    20761 tggtttgatt gccggcagtg tcacgatctt gaacttctgc caagcatttg caccgtccat
    20821 ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa atgaaataaa
    20881 cgcatacgga tagtatctcc agatagcaaa caaaacgacc ataaagaagc tagcaactgg
    20941 ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac ctatctacta acagatcatt
    21001 gacgattcca taactattat tgaacatata tttccaagca aaaatcaaag aaatagatgg
    21061 cgtaacataa cttaataaaa tcagtgaacg caccgttttc ctaaatctga acttgcggtt
    21121 aagcaaaata gctacggcta atcctaaagc tgtactgcct gctacaacta agaatgtgta
    21181 agcaactgtt aaacctaaag aacgataaaa ttcactatca cttaaaattt caagataatt
    21241 gcttaatccg ataaaaacgg cgtctgagtt aggattcagc ggcatatcga aaaaactgat
    21301 ctgaatattg gaaagcattg gaatcagcac taaaacagct aataaaagca tgcttggtgc
    21361 taaaagtccc atggctaatt ttaagtcaga atgtttcctt ggacgattca ttgaaacctc
    21421 cccctgataa tctgccaata gctatctact tttttcgaac accattggca gaacttcttg
    21481 ttatttcgta tttttttgtg cgtcagctaa agcttgatcg acatcttcgt ttccgacagt
    21541 tacttggttc actgcttttc cgatcacacc tgagcttgtg atatctccca ttttcgtgaa
    21601 attcttttcc ccgactagtc caaagacttg tacttcatca aaagcactag caatctcaga
    21661 tggcagctcg ccaaaagact tgatgacttc attttctttg taaccatcca gttcgactac
    21721 ttgtttattc actggttgag caccaccagg agacatcaag acccactttt ccatgttttt
    21781 cggttgagat aagaaagctg tgaatgcttc agcagcctct ttttgtttct cgtctagacc
    21841 agaagaaatc gtcaatccag atactgtgcc atagacagct tgatcttttt gtgttgggat
    21901 agcaaaaccg atattttctg agttccccgc ttcataaaca gaaggcaaaa tgtaagttga
    21961 atagattgcc attggtgctg ttccattcat gaaagcatct ttgatttctg tcacatcatt
    22021 agagcctggc atcgtgtatt gggataagtt ttgataaaac gacaatgctt ctctcatctt
    22081 ttccgtatcg atcgttacct cgccttcatc atccaaaaca tttgctccat ttgacaaagc
    22141 aaattgagag aacgcttgct cactcattgt tccttcagca gtaggcatag caattccata
    22201 ttgtttattc tctttatctg tgaaatgttg agcaattttt aaaatatcat cccaattttc
    22261 tggttctgaa aaaccagctt ctgataattt ttctttgtca taccaaatac cttgaaccca
    22321 gccgctaatt ggtgcagcaa tatagctttt tccatcttct gaacgaacaa ggtttttggc
    22381 tccgtcataa taattatctt ctccagcttc ctcgataacg ttttggactg ccgtctgatc
    22441 aatcagttca tctttatcca tcactttagc aaaatcctga ctgacttcca ttacttctgg
    22501 taatttaccg gaacgagcta acgtaacgat ttttgtattg aacgctgatt cttctaccgg
    22561 cacttgcttg attttgatgt ctggattttc tttttcaaaa tcagcaacca gttcattgat
    22621 cacacttaat cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg tctgttgtga
    22681 ttcgttagag ctttgacttt caccacaagc agtaagaaga aatccagtag ctagcagaca
    22741 ggtagtaaca ataatttttt tcattttttt ccacctctta ttttattatt ttcagccaca
    22801 tatattcata cggctggaat atcaactttc gttcttgact gcaatggata atttgtccag
    22861 aaaacaggtc taggtaatct ccttttggta aaagataacc tgctgattga ttggaaaaat
    22921 tatggataac taagactttc tcttccttcg cttcgcgaat aaaactgata aaagaatctg
    22981 ctgagtccag taagtaaatc aaactattcg gatggaaggc tttttctttt tttcgaatag
    23041 atagcatctg ggtcaattca ccatatactg ctgttcttaa tggatctgtc tgcaactctt
    23101 tctcaatatc tgctaacaaa aatttttgcc gattgatttt tcgattctct cctgtttttt
    23161 ctacgccttt gtaatcattt cgacttccca gcacactttg aatataaatt gccggcacgc
    23221 ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc cattccgtcg
    23281 tatctgttgg agaacttaac gcatctagat acgtagcgtt tatctcatat ggactggtcg
    23341 ttccatctgg gtttttcttg tagtttactc gggtaccctc tttcttcagc tgttccacta
    23401 attcaaggat cgttttttcc ggcagaatac ctcgtaccgg attcaatccg ataccgtcat
    23461 gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg gtttttaacc
    23521 attctttcaa cttacttgta ttacccgaac gaatcgcttg aagggtcaaa ggtggtagcg
    23581 aaaactgata gaccatctgt gcttcattct cgccattccc aaaataactg atattatctt
    23641 tgtgtggcac atttgtctca gtgatcaata ccgttcctgg agctgcttca tcagcgattg
    23701 cacggaataa ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa gttcctagtt
    23761 ctttccataa aaatcccaca gcgtccaagc gaataaatga agcgcctttt tctaagtaaa
    23821 acaaaagaat atcgatcatt ttcaataaaa cttctggatt tttaaaattc aagtctactt
    23881 gatcttcgct aaaagtcgtc catacgtgtt tttctttccc gtttttgtca atgaacgttg
    23941 taagtaacgg gctggtcctt ggtctagtca cgctgcttgt atctgtatcc agtggcaaat
    24001 caataaaaaa atctttgtac gtatcattca agtttaaata ttcctgaaac caagtacttt
    24061 cggcagacat atgattgcag acaaagtcga acattaaccg tgtttcgctt ctcatttctt
    24121 ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg ataatttaca acggagaaac
    24181 catcatcaga tgtatatggg aaaaatggca agaaatgtac aactctaaat gtatctgcca
    24241 aatacttttg ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct tcaaaaaatt
    24301 gatccccata agtaatcaag aaaatatctt cttcgtccca atgcagtttg cctgcctggc
    24361 ctttcttctg ctgataaaca ctgacttttt gttcaatcat agaggcggtt ttctcagaca
    24421 aatctgagtc gcgataaatc gcttgtaaaa gttttgtata catcggattc ccctttcttt
    24481 tttgggagcg ttcccaacga tttgatcata ttatggaaaa cgcttactgt caatagcatt
    24541 tttttaaata attttgatat acacaaaaat acaaataaga aagcttatgt atcaataatt
    24601 aatagcttct taaaaaaata tggtctctca tgatcatgca agctattcct aaattttcaa
    24661 aatcatagac aaaaaagaat gtctattgta ctatggaatt attctaaaac gctcccaaaa
    24721 aggaggtgca tataaaattg aaacctacta tttatgacat tgcgaaaaaa gctaatgtct
    24781 caaaatcaac tgtctccaga gtattaaata accagccaaa tatctctgat aaagcaaaag
    24841 caagtgtact tcaagcaatc aaagaattga attatgaacc gagcaaaatt gcaagaggac
    24901 tgtcaggaaa tggttttgac gcgattctag cattatctca tcgtacttct catacgacct
    24961 atggcaatcc ttttttttca gatgttattc aatccatttc tgccgtttct gaacaaaata
    25021 acttcgactt gattctacaa acggcaaaaa caccagaaga agagctagaa aaatgcgcga
    25081 aaaagatcca agaaaaaatg atccgaggaa ttattatttt aagctctacg atggatgaaa
    25141 cacttctttt tgaattggat caatacaata ttcctattgt ggtaatcgga aaagtcggaa
    25201 acgactacac gaacatctac tcagtggaca cggataattt ccaggatagt tacgatttgg
    25261 tggaaacact gatcaagaat ggacaccgtc atatcggctg tctccatgct ccgacagaag
    25321 tttatgtatc caatgatcgt ctcaatggtt accgtcaatg cttatttgat caccacctag
    25381 atatccgtaa tgaatggatt atagacggcg gctttcgctt agaagatagc atggcagcaa
    25441 tgaatgattt aatgaagcta cgagaaagac caactgcgat tttttctacc gatgcattaa
    25501 aatcattgag tatttacaaa gtggcagaac aatctggaaa gcagatacct gatgatcttt
    25561 cgatcgcagg attcagtgat cccttattgt cctccctatt ttcgccacag ttgactcgga
    25621 tagacattcc gacaaatcag ttaggaatca aagccacaga acttttattc caactaatcc
    25681 accaccaata ccctgaagaa aaaagaatca ttctttcaac cgagattact ctcacagatt
    25741 cgatccaaca acaagaaatg tagaacagaa ttataaaaga acagaaacac tgaggataag
    25801 tcagttactc tgttcttttt tcaatcactc atccctcgtt accccctccc ggtattttag
    25861 cgaatttttc ttattttcca tctgttttag atagctgtgg ataactataa tgtttgtgtt
    25921 aaactatttt ttccacaggg aagaaaaaac tatttttaac cacgttgtgt ataagttttt
    25981 atagttcgtt tttttaaaca catttaccct tatttcaatc catgttattt ggggatactt
    26041 atctctaaaa cataaataga ataacagaca tgtctactgg ttttgtttta ttcgaggaat
    26101 tattcaataa tagctagcaa aatattaaaa ataaccgctc tatcttccca tcaatttatt
    26161 ttgcattcaa aaaatgatct atctttactt ttgttgattg acttctcttg gaagatgatc
    26221 gttccgatac cttaattgga tacgaaccga tgttgttaag atcctttccc acttttcttc
    26281 tagttagaca aattatttta gacaaaccaa aaaagaggtt gtgacaataa tttgtcacaa
    26341 cctctttttt aaaatatcta ttttgtttga ttgttcattg cgtcttctgc agccatttgc
    26401 gcttcaatat ctgaaatact gtagacattt tcgcttgcta caccgacttc ttttggttcc
    26461 atgtcacgat aacgttgcat accagtacca gctgggatga tcttacctat aataacattt
    26521 tctttcaaac caagaagagg atctttctta ccgcggatcg cagcatccgt caagacacga
    26581 gttgtttctt ggaatgatgc agcagataag aaactatttg tttctaatga tgcttttgtg
    26641 atacctagta agacaggtcg agaagttgca ggaacgcctc ctgagactag cgtattgtag
    26701 ttttgatctt taaagtctgc gatgtctaat aatgtacctg gtaagatatc tgtatcacct
    26761 ggatccatta cacgaacttt acgcagcatt tgacgaacca ttacttcgat atgcttgtcg
    26821 ccgatttcta ccccttgcat acggtaaaca cgttgtactt cacgaagcaa gtagttttca
    26881 acagataata catcgcgaac ttgcaacaat tgttttggat cgattgatcc ttctgtcaat
    26941 ggtgcaccac ggtggatcat atcgccttct gctactttca tacgtgctgt ataaggaaca
    27001 gtatacgtac gtgtatctgt ttttcctttg attgttactt ctttggtacg tgttgcagga
    27061 tcttctgaga tatcgatgac atcccctgtt acttctgtga tcactgcttg ccctttcgga
    27121 ttacgtgctt caaagatttc ttggacacga ggaagacctt gagtgatatc gtctccggca
    27181 accccacctg tatggaatgt acgcatcgtc aactgagtac caggttcacc gattgattga
    27241 gcggcgatcg taccaactgc ttctccgact tcaacgtcag agccagttgc aaggttgcgt
    27301 ccgtaacaat gtttgcagac accatgtttt gtattacatg tgaagacaga acggattgtc
    27361 actgtttcga cacctgcatc aacgatttgt tttgcgatgt cttcggaaat caattggttt
    27421 cctgcaatga tcaattcatt tgtttcagga tgacgtactt ctttacgggt gtaacgtccc
    27481 actaaacgat cttctaatga ttcgatgatt tcatttccct cgcggatggc ttggatttca
    27541 agacctcggt cagtaccgca atcttcttca cggatgatta catcttgggc cacgtcaacc
    27601 agacgacgag tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt catcccttta
    27661 cgagccccgt gagttgagat aaacatttcc aagacggata gtccttcacg gaagttcgaa
    27721 atgattggca attccatgat tcgaccatta ggagcggcca tcaaaccacg cattccggca
    27781 agctgtgtaa agttggaaat gttaccacga gctccagaat cagacatcat gaagattgga
    27841 tttttcgctt ccaaaccttc catcagtttt tgttggatgc tgtcttttgc agcattccaa
    27901 acagcaatga cacgttcata acgttcgtca tctgtgatca gaccacgacg gaattgtttt
    27961 gtaatagttt caacttgttt atgggcattt tcaatgattt cttgtttttc attcagtaca
    28021 ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg aatgtttgta ccctaagtct
    28081 ttcatacggt caagcatctt agaagtttct gttactttga atcgtttgaa gacttctgcg
    28141 atgatgtttc ctaagttttt cttcttgaac ggtaatacta attcttgctc tttgatatgt
    28201 gcaggaatat cagtaccagc ttcaacaaag tatttgtccg gtgtttggac ggtcaagttg
    28261 aagtctgttg gttcattcag ataagggaat tcaggaggca tgatctcgtt gaagatgatc
    28321 ttaccaactg ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg tttttcacct
    28381 aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt gcgccatgcg
    28441 ataacggctt cgttttgatc tctgaagacc attccttcgc cttcgcgtcc ttcttcttcc
    28501 attgtcaagt agtagttacc tagaaccata tcttgggaag gagtaacaac tggtttacca
    28561 tctttagggt tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc tgcttgtgct
    28621 tcttcattca atggaacgtg gacagccatt tggtctccat cgaaatcggc attataagct
    28681 tcacatacaa gtggatgcaa acggattgcg cgtccttgta ctaaaactgg ttcgaatgct
    28741 tggataccaa gtcggtgaag cgtaggtgcc cggttcagca atactgggtg ttccttgatt
    28801 acgtcttcta aaacatccca tacttcgtct tcttgacgtt cgattttacg tttcgcattt
    28861 ttgatgtttg aagctagttc gcgttgaaca agctcacgca tcacgaatgg tttgaataat
    28921 tcaatggcca tttcttttgg aagtccacat tggtacatct tcaagaaagg tccaacaacg
    28981 ataacggaac gacctgagta gtcgacacgt ttacctagca agttttgacg gaaacgacct
    29041 tgtttccctt tcaacatatg agaaagagat ttcaatgggc ggttacctgg tccagtaact
    29101 ggacgtccgc gacgaccgtt atcgatcaat gcatctactg cttcttgcag catacgtttt
    29161 tcattttgaa cgatgattcc aggtgcattc aagtctaaca aacgtttcaa acggttatta
    29221 cggttgatta cacgacggta aagatcattc aagtcacttg ttgcaaaacg tccaccttct
    29281 aattgaacca ttgggcgtaa atctggtgga ataacaggga taacgtccat aaccatccag
    29341 cttggttgat ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg gattgcacgt
    29401 gtacgttttt gtccttgtgc agatttcaat tcttctttca attcggctgc ttctgcatct
    29461 agatcaacat tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc ttggaattct
    29521 tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg ttttttctct
    29581 aaattcgtat ttcctggttc gatgactaca tatgaagcaa aataaatgat ttcttccaaa
    29641 gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag gaatcccttt aaagtaccag
    29701 atatgtgaaa caggagctgc taattcaata tgtcccatgc gttcacggcg aactttcgaa
    29761 cgagtaactt ccactccgca tcgatcacaa acgattcctt tataacggat acgtttgtat
    29821 tttccgcagg cacattccca gtcttttgta ggaccaaaaa tccgctcaca gaacaaccct
    29881 tcgcgttcag gttttaaggt acgatagtta atcgtctcag gtttttttac ttcaccgtaa
    29941 gaccagcttc tgattttttc gggagaagcc aaacctattt gcatactttc gaatttattt
    30001 acatcgatca aaaggggttc cctccatttc atttaatggc aaaccgcaag acggctgatc
    30061 ttcgaccgcc ttgcagctca tagaccggat tagtctaaat tatcttctgc ggtttcaaag
    30121 ttttggatga catcgtcttt ttcatcttcc acttgttcag cagctttctt ttctaattct
    30181 ttggcagttt gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa atcgtcgtct
    30241 tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga tatccaagac acgcatgtcc
    30301 agacctagtg attgcaattc tttcaccaat acgcggaatg attccggtac acctggtttt
    30361 ggaattggtt cacccttgac gattgcttcg tatgttttca cacgtccgac tacgtcatct
    30421 gatttgtatg tcaagatttc ttgtaaagta taagccgcac cgtaagcttc cagtgcccat
    30481 acttccattt caccaaaacg ttgtccacca aattgagctt tacctcccaa tggttgttgt
    30541 gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac catgtgagca
    30601 agtttgatca tgtacatcac accgacagac acacgtccat caaatggttc acctgtacgt
    30661 ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg cttcacgaac tgtttcccat
    30721 acgtcttcat cacttgcacc atcaaatact ggcgttgcta cgtggatacc taactgacgt
    30781 gcagccatac ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga aggcacccct
    30841 aaaggattca gcatgatatc gattggtgtt ccatcaggta ggaatggcat atcttcttcc
    30901 ggcataatac gggatacaac ccctttattt ccgtgacgac cggccatttt atccccttca
    30961 tggattttac gtttttgaac gatataaaca cgaactaaca tgtttacgcc tggtgacaat
    31021 tcatctccag cttcacgagt aaagattttc acatcgtgaa cgataccgcc gccgccatgt
    31081 ggtacacgta atgatgtatc gcggacttca cgagcttttt cgccaaagat cgcatgcaat
    31141 agacgttctt ctgcagataa ttctgttacc ccttttggcg tgactttccc tacaagcaaa
    31201 tcgccatctt ggacttctgc accaatacgg atgataccca tttcgtctaa atcttttaat
    31261 gcgtcttccc cgacattagg gatttcgcgt gtgatttctt caggtcctaa ttttgtatcg
    31321 cgggcttctg attcatattc ttcaatatgg atagaagtgt atacgtcatc ttttaccaaa
    31381 cgacggctca tgatgatcgc atcttcatag ttgtaccctt cccaagtcat gaaaccaact
    31441 aaaacgtttt ggcctagagc catttcgcct tgttccatag atggtccgtc tgccagagta
    31501 tccccttttt cgactttctc acccaaatga acgattggac gttggttgta gcttgttccg
    31561 gagtttgaac gacggaattt tgttacatcg tatttatcta atgcaccgtt gtcgcggcgt
    31621 acgcggattt cagaagcatc aacgtattca acgacaccgt catgtttaca taacaaagca
    31681 gctcctgagt catgcgctga tttgtattcc ataccagtac caacccatgg tgcttgagga
    31741 ttgatcaacg gcactgcttg acgctgcatg ttggcaccca tcaaggcacg gttggagtca
    31801 tcgttttcca agaaaggaat acatgctgtc gcaacggcaa ctacttgttt aggtgatacg
    31861 tccatgtagt ctactttatc aatagatact tctaagtttt cactttgagc acgagccata
    31921 acaacgtctt gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg ggctacgatg
    31981 taatggtctt cgatatcagc tgtcaagtag tcgatttggt cagtaacacg acctgtttca
    32041 cggtctacac gacgataagg tgtttcgata aatccgaatt tatttacttt cgcataactg
    32101 gacaaactat tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg acacatacgg
    32161 ccatagtggg agtagtgaac gtcacgaact tcataaccgg cgcggtcacg agtcaatccg
    32221 ccaggtccta aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg gtttgtttga
    32281 tccatgaact gtgacaactg agaagaacca aagaattctt tgatacttgc cacaactgga
    32341 cggatattga tcaattgttg aggtgtcaat gtttcagtat cttggatcga cattctttca
    32401 cgaaccacac gttccatacg tgctagcccg atacggaatt ggttttgtaa aagttcaccg
    32461 actgaacgga tacgacgatt tcctaagtgg tcgatatcat cgacattgcc gataccttcc
    32521 atcaagttca agaaatagct cattgaagca atgatgtcgg ctggacgaac cgtttttaca
    32581 gctgcttctg gatagccgtt tccgatcacg tttacttcac gttcagggtc tcttggtgaa
    32641 aatactttga tgacttggac agtcattgga tctgtgacca caccgtcttc tgatggatag
    32701 taagtcactg aatttaggcc attatcaatg aaaggagcta gtgtttccat gacttggtga
    32761 gtcagaactg ttcctttttc aacgatgatc tcgcctgttt ctggatcaac taatgtttca
    32821 gctaatgtca gattcaatag acgtgttttc aaatctaatt ttttgtttac tttgtaacga
    32881 ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac gagcattcag caagttacgt
    32941 gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc tttcaaacct
    33001 tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt attgcgtaat
    33061 gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc taacgcacga
    33121 actaatacgg ttaaaggaat cttacgtgta cggtcgatac gtacataaga gatgtctttt
    33181 gcatcagttt ccatttctag ccaagctcca cggttaggga taacagtaga accgaatcct
    33241 tcttttccgt ttttgtcgac ttttccatgg aaatagacac ctggtgaacg gactaactga
    33301 gaaacgatta ctcgctctgc cccgttgatg atgaatgttc cttgttctgt cattaatggg
    33361 aaatcaccga agaatacttc ttgtgctttg atttcgccag tctcgcggtt tgtcaaacgc
    33421 aatgttacat gtaatggtgc agagtagttt gcatcatgcg cacgagcttc tgctactgta
    33481 tactttggtt ctttcaattc ataatctaca aattccaaag atagattgcc attgaagtca
    33541 tcgattggca aaatgtcttc aaacatttct ctaagtccct catctaagaa ccattggtaa
    33601 gagtctgttt gaatttcgat caaatttggt aattccaaca cttcactgat tcttgcgaaa
    33661 cttctacgtt cgcgatgttt tccgtatttt actacgtgtc cagccaagct cttcacccct
    33721 ctaaaatgtc attcaaaaag actacccgat caattgttac aataatattt caaatattat
    33781 ttttaggtaa caacagacat tttttgcggc ttttaggcaa aaaaaaacca aaaatagaac
    33841 attctcttga aaccttactt cgtgagcttc tacttttgtt tccttatatt aaagccgaaa
    33901 cggacaatat ttcgtttctg ccttttcttc atcagatagt ttttgcaact tcatatacta
    33961 cacttatttt ctataaaagt caacacttcc tggcgtttct tctgcttttt cgcaacattt
    34021 tcttaatgtt ttcaatttta tgttttcaat tatctgacaa ttcttaattg acagaccttt
    34081 ttatctcggt tatactttag aaaattcatt ttgtggagga acttctatgc gaaccaacat
    34141 caaccaaatc gcaccaacac aactgaataa cttttacttt agctacttta gataggtttg
    34201 tattcatcat gatggataca aacgttaaac gacagtttgt atctatgatg gctaaagaac
    34261 tttgtattgc agcaaaaagc cacgtagaca aaatgctaag tggctgagca gtgaaaatac
    34321 aaagtaaaag tatcaagcat tctacttttt caatttattt tccgactttt caatccattt
    34381 ggacattttg tctgaatgga ttttttgttt cccaaaatta aggaggaaaa agaaatgaaa
    34441 aaaaatataa tcatcggatt aggcgctgcc ctgctactag ctttaggagg ttgcgcacaa
    34501 aaagacaatt ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt gcaacttgta
    34561 caacataact cgttagatgc tgcctataaa ggattcaaag aaggattagc agaagaaggg
    34621 tataaagaag gcgataatct aacaatcgat tatcaaaatg cccaaaacaa tcaagacaac
    34681 ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg atttgctact tggaatcgcc
    34741 actccagcag cacaatcttt attaaatgaa acaaccacta tcccgatgac tgtcacagcg
    34801 gtcacagact tggaacaagc ccatttagtc acatccaatg aaaaacctgg cggcaatgtg
    34861 actggcacga cagatatggt gccaatcgaa aaacaaatcg gcttactgtt aaatatcgtg
    34921 ccagacgcca aaacaatcgg catcatgtat aacaacggtg aagcaaactc taagatccaa
    34981 gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt actgacagca
    35041 aattccacaa atgatgtcca acaagtgacg aaaagtctag ctaaagatgt agatggaatc
    35101 tatattccta ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga agtcgcaaaa
    35161 gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg cggtctagca
    35221 acagtaggaa tcgactacga aagcttgggt aaacaaacag ggatcatggc agcaaaaatc
    35281 ttagacggca agaatccaag tgaacttcct gtcgaaacag cgaatgattt ggaactgtat
    35341 gtcaataaag acatggccaa agcattaggc atcaatccag acagcatcaa agaagctgaa
    35401 taaaacacag gaggattctc atgacaaatt tgctctctct tttcttatca tctgcatcgc
    35461 aaggggtctt atgggcccta cttgcgattg gtgtatacgt cactttccgt atcttggaca
    35521 ttgccgactt gactgctgaa ggaagctttc ctttaggtgc agggatcgcg gcagtaagta
    35581 ttacaaatgg ctatcatcca ttgacagctt gtcttctagg ctttttaggg ggtgcggcag
    35641 cagggcttgt ttccggactg cttcacacaa aattgaaaat tcccgccctt cttgcaggga
    35701 tcatcaccat gactggttta tattctgtca catcccgtgt gatgggtgcg ccgaacatct
    35761 ctttattggg tcagccaacg atctttcatt gggtcgagtc attcggcata acaaaagaca
    35821 acgctgtact gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta cttgttttgt
    35881 tcttacgtac agaaacagga cttgctattc gtgcaactgg cgataatctg gcaatgagcg
    35941 aagcaaacgg gatcaacaca gataatatga agatcatcgg ttatatgatt tctaatggat
    36001 gcattgctct ttctggctcc ttgcttgcgc aaaacaacgg ctttgctgat ttaaattctg
    36061 gtattggaac gatcgtaatt ggacttgctt ctattatcat cgcagaagtt ttgttacgta
    36121 accagccact atttctccgt ctcttgactg tcgttttagg tgcggtcatt tatcgtttta
    36181 ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt gcttctgctt
    36241 tcgtgctagt gatctgtctt tctttcccgc agatccagca aaaattccat ttacctcaaa
    36301 aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc tccatcatac
    36361 ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc tttccttaga
    36421 tcccggcgac ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta ctttgctgaa
    36481 cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg gaaaaaatat
    36541 cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc aagatcctaa
    36601 actagggaca gccgttcgtt taacggtaga agaaaatctt gctcttgcta tgaaacgagg
    36661 aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac cgaacatttt tcaaagacca
    36721 gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg gtttgctgtc
    36781 tggaggacaa cgtcaagcta ttacgttatt gatggccaca ttacagcgtc cagaactgat
    36841 cttattagat gaacacactg cagcgcttga ccctgctact tccaaaacgg tgatggaatt
    36901 gacagaaaag ctgatcactg agcagaatct cactgctttt atggtcactc atgacatgga
    36961 agacgcgatc cgctatggga gtcgactgat catgctgcat caaggaagga tcgtcgtaga
    37021 tatccagcaa gaggaaaaac agcagctcac agtgccccaa ttattagcgt tgttcaaaca
    37081 aaacagcggc acggcattaa aagacgatca agtactttta tcttcataat cagaaagagg
    37141 atgggacaaa aaatgaacgc ccaccctctt cttttttctc tttccttctt tcgtttttgc
    37201 tttatgtatc aaccgttgtt tcttcgaaaa ctattttgtt ctctccttct tcctgataga
    37261 atggatggga aaggagcttg atgccatgac aacgaaagac aaggtacttc aattattgaa
    37321 aaagacacca gattttcttt ccggagaaaa gttggcacag caactggaag tttcccggac
    37381 aagtatttgg aaagccatca aagaattgga aaaggcaggc tatcgttttg agcacgggcc
    37441 tacagggtat cgttacctgc cctctgacgt actagatgca aaagaaatcc agcaagagct
    37501 acggtcgttt attcctgatt tgaaagtaca aacgatgttg acttctgaat ctacgatgaa
    37561 agatgtcaaa ctggcagcag ctcaaggaga ttctgtttcg aagttatttg ttgctgatac
    37621 acaagaacaa gctcgtggac gttttggacg gccttttttc gctgaacctg gacgaggaat
    37681 ctacatgagt ctcttgctcc atccagacaa acggtttgat gaattgcctc aatatacgat
    37741 catcgcagcc agtgcctgtg tgaaagccat tgaatctgta accggtcgtt ctgcttcgat
    37801 caaatgggtc aatgatatct acatggatgg aaaaaaagtc tgtgggattt tatctgaagc
    37861 agtcagtgat atggaaagcg gcaggatcag tcatatcatc attgggatcg ggcttaattt
    37921 ttctgttcct caaaatcatt ttccagctga tctccaagaa aaagccatct ctgtctttcc
    37981 cgacggacaa gctagtgtca caaggaatca gctaatcagc aaaatctggc aagaattgtt
    38041 cacttatctt tctcacccag aaaaagcgtt ggacttctat aaagaaaaat cattcgtatt
    38101 gggaaaaacc gtgtcgttca atcaaaaagg aacgacttat gtcggtcttg caaaagaaat
    38161 cggtgatcat ggcgaactga tcgtccaaac aaatgaacag aagatgagtt tgtcttctgg
    38221 cgaaatcagt ctcacatcta tccgtacgca ataattatca gatttcacaa aaggacacct
    38281 gatctcatac agtgtccttt tttctgaatt ctcttcaaaa gactctcctt attctatcag
    38341 ctgacagttt ctctatcctc tagaaaaagg tattctaaaa atatctataa agcatggtac
    38401 actttcttta aatgaatcaa tgcaaaaaac aagagagtca tcagtttaca cgatcaaaag
    38461 agatagcagg ccgcttcctt tttcaatctt atagatagac catgggtaac gataaaaaca
    38521 aaaaagaact tgcgacttat tttgccgtaa gttctttttt agctagtgcc ttaatctctt
    38581 aatcccaaca aacgtctcgc atagatcacc tgaccagagt gcatagcagc atcatcaata
    38641 atagaaacga gacgcattcc tcgtgtgact ggtggtgtcc agttctcgtc aattacttca
    38701 tctagctcgg aaggttccag ccttcttaag taagaaatag caaaatcagc agctcctcgc
    38761 aagtatccta gcagatcttc tatttgatta gcttggatct gctttgcttg ttccagacta
    38821 tgcttccaat cttgttcgtt atctgctatc gtcatcggaa accggctttc ccagtcttga
    38881 ctatgccaaa ttggagaagt tcctgctaaa tcagcaatct ggaaatctaa ttctcgagcg
    38941 gtatgccaag ccagccaagt cattgatttg atctgcgggg ctctatcttc cactggaaaa
    39001 cgattggcct cctcaataga gacgccttca aacaaacgtt caaaccgatc gtatgcccga
    39061 tccacactct cgcatagcat atctaaataa tccatttatt ttcctccttt ttatttatcc
    39121 tacttacttt ttcctaattc taggatacct acttatatgt tactacacac ccttattttt
    39181 tacactttaa cagtcaccat catcaatagt ttggagattg aaagaaaaag atagtgtcga
    39241 actatcaatt gaacaagaag caacaatatg aacgtttcat taatattctt taaataaaca
    39301 atgatttttc aaacgaacag tttaagacta ggagaaaaag agtataatag gaaaatgttt
    39361 gaaaggaaag gaaggggaaa aatgaaaaaa agggcaagtt tactagtcct agttgctttg
    39421 ttgttcaata tcttttcgta tggtgtggcc gtttcagcgg aaacatacat tcctaaagac
    39481 cctgggaata tttataatga tctaactggt aactatggct tgttaggaat tgctagtcaa
    39541 ttccacgtat ttgctaaaaa taagacaact gtgaatgcac atacagacgg aaatatagca
    39601 acacatgaac ttgatgcaaa aaataatttt ggtacagagc ttcatgaagg tttattgaac
    39661 caagaaataa attatataca aaaaactgat tctttcgcta attcttcagg catacctaca
    39721 ggtagcacaa gaatgaataa atttgtcgtt ggcagtaacg tggatgtagc acttcaagat
    39781 ggtcatccta cagtcaatgg atccaggctt gatcacttaa ctccagatga tttttataaa
    39841 gatcgtgctg ggcatgaata tattgatttc gagcaggaat ttgctaaatt gaatgtagca
    39901 gcaaatgatc ttgcaacgat cactccagct aaaacatata ctgctgcaga ttttccagac
    39961 atgaacaatc ggacaatcga tttaactgat ttatcagata ccgggttttt attggtaaat
    40021 atcgatgcag aagtgttaac gatgaatacg ccacttcaaa tcattaatcc aaatgatcaa
    40081 gtagttgtat ttaatgtcat caacagttct tctgcattga acgtacagtc accaatcaaa
    40141 tacaatgatc gttcaaatca tgaaacagaa gatttttcgg atgcgaatat ctcttggaat
    40201 tttggaaatg agatgactga tttgacaatc agtgctcctt ttcaaggaac aatccttgcg
    40261 cctaatgcta ctatccgcat tactcaaaac caagacggaa cgattatagg aacaaatgtg
    40321 attttagatg cagctaccaa tcgttgggat cctaacgaaa tatttatcag cgacaatggt
    40381 acagatacga cagacacaag cgacagtaca gatacatcta ccacaagtga tagctcagat
    40441 tcatcaactt caagcgacag tacagatact acctctacca caagtgat
//
LOCUS       DAZKMK010000013        38560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000013 DAZKMK010000000
VERSION     DAZKMK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.013"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..1022)
                     /locus_tag="ACG8V1_000737"
     CDS             complement(147..1022)
                     /locus_tag="ACG8V1_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0328854.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1131..2900)
                     /gene="aspS"
                     /locus_tag="ACG8V1_000738"
     CDS             complement(1131..2900)
                     /gene="aspS"
                     /locus_tag="ACG8V1_000738"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0328855.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2915..4207)
                     /gene="hisS"
                     /locus_tag="ACG8V1_000739"
     CDS             complement(2915..4207)
                     /gene="hisS"
                     /locus_tag="ACG8V1_000739"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0328856.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4601..5548
                     /locus_tag="ACG8V1_000740"
     CDS             4601..5548
                     /locus_tag="ACG8V1_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0328857.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5637..6896)
                     /locus_tag="ACG8V1_000741"
     CDS             complement(5637..6896)
                     /locus_tag="ACG8V1_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0328858.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7067..7513)
                     /gene="dtd"
                     /locus_tag="ACG8V1_000742"
     CDS             complement(7067..7513)
                     /gene="dtd"
                     /locus_tag="ACG8V1_000742"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0328859.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7524..9737)
                     /locus_tag="ACG8V1_000743"
     CDS             complement(7524..9737)
                     /locus_tag="ACG8V1_000743"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0328860.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9970..10734)
                     /locus_tag="ACG8V1_000744"
     CDS             complement(9970..10734)
                     /locus_tag="ACG8V1_000744"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0328861.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10735..11682)
                     /gene="prmA"
                     /locus_tag="ACG8V1_000745"
     CDS             complement(10735..11682)
                     /gene="prmA"
                     /locus_tag="ACG8V1_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0328862.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11695..12183)
                     /locus_tag="ACG8V1_000746"
     CDS             complement(11695..12183)
                     /locus_tag="ACG8V1_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0328863.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12149..12820)
                     /locus_tag="ACG8V1_000747"
     CDS             complement(12149..12820)
                     /locus_tag="ACG8V1_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0328864.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12958..14244
                     /locus_tag="ACG8V1_000748"
     CDS             12958..14244
                     /locus_tag="ACG8V1_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0328865.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14316..14510
                     /gene="ssrS"
                     /locus_tag="ACG8V1_000749"
     ncRNA           14316..14510
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8V1_000749"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14588..14872
                     /locus_tag="ACG8V1_000750"
     CDS             14588..14872
                     /locus_tag="ACG8V1_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328866.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14945..15424
                     /locus_tag="ACG8V1_000751"
     CDS             14945..15424
                     /locus_tag="ACG8V1_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0328867.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15647..15790
                     /locus_tag="ACG8V1_000752"
     CDS             15647..15790
                     /locus_tag="ACG8V1_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0328868.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16135..16263
                     /locus_tag="ACG8V1_000753"
     CDS             16135..16263
                     /locus_tag="ACG8V1_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328869.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            16368..17951
                     /gene="abc-f"
                     /locus_tag="ACG8V1_000754"
     CDS             16368..17951
                     /gene="abc-f"
                     /locus_tag="ACG8V1_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0328870.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18388..19572)
                     /locus_tag="ACG8V1_000755"
     CDS             complement(18388..19572)
                     /locus_tag="ACG8V1_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0328871.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19597..20604)
                     /locus_tag="ACG8V1_000756"
     CDS             complement(19597..20604)
                     /locus_tag="ACG8V1_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0328872.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20776..21135)
                     /gene="comGG"
                     /locus_tag="ACG8V1_000757"
     CDS             complement(20776..21135)
                     /gene="comGG"
                     /locus_tag="ACG8V1_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0328873.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21107..21532)
                     /gene="comGF"
                     /locus_tag="ACG8V1_000758"
     CDS             complement(21107..21532)
                     /gene="comGF"
                     /locus_tag="ACG8V1_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0328874.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21495..21842)
                     /locus_tag="ACG8V1_000759"
     CDS             complement(21495..21842)
                     /locus_tag="ACG8V1_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328875.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(21814..22284)
                     /gene="comGD"
                     /locus_tag="ACG8V1_000760"
     CDS             complement(21814..22284)
                     /gene="comGD"
                     /locus_tag="ACG8V1_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0328876.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22259..22537)
                     /gene="comGC"
                     /locus_tag="ACG8V1_000761"
     CDS             complement(22259..22537)
                     /gene="comGC"
                     /locus_tag="ACG8V1_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0328877.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22534..23583)
                     /gene="comGB"
                     /locus_tag="ACG8V1_000762"
     CDS             complement(22534..23583)
                     /gene="comGB"
                     /locus_tag="ACG8V1_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0328878.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23540..24469)
                     /gene="comGA"
                     /locus_tag="ACG8V1_000763"
     CDS             complement(23540..24469)
                     /gene="comGA"
                     /locus_tag="ACG8V1_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0328879.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24601..26355
                     /gene="ade"
                     /locus_tag="ACG8V1_000764"
     CDS             24601..26355
                     /gene="ade"
                     /locus_tag="ACG8V1_000764"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0328880.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26453..27115)
                     /gene="trmB"
                     /locus_tag="ACG8V1_000765"
     CDS             complement(26453..27115)
                     /gene="trmB"
                     /locus_tag="ACG8V1_000765"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0328881.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27185..27967)
                     /locus_tag="ACG8V1_000766"
     CDS             complement(27185..27967)
                     /locus_tag="ACG8V1_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0328882.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28171..29382)
                     /locus_tag="ACG8V1_000767"
     CDS             complement(28171..29382)
                     /locus_tag="ACG8V1_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0328883.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29375..30112)
                     /locus_tag="ACG8V1_000768"
     CDS             complement(29375..30112)
                     /locus_tag="ACG8V1_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328884.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30250..30684
                     /locus_tag="ACG8V1_000769"
     CDS             30250..30684
                     /locus_tag="ACG8V1_000769"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0328885.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30677..31021
                     /locus_tag="ACG8V1_000770"
     CDS             30677..31021
                     /locus_tag="ACG8V1_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0328886.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31201..32211
                     /locus_tag="ACG8V1_000771"
     CDS             31201..32211
                     /locus_tag="ACG8V1_000771"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0328887.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(32280..33224)
                     /locus_tag="ACG8V1_000772"
     CDS             complement(32280..33224)
                     /locus_tag="ACG8V1_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0328888.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(33208..35928)
                     /locus_tag="ACG8V1_000773"
     CDS             complement(33208..35928)
                     /locus_tag="ACG8V1_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0328889.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(35925..37145)
                     /locus_tag="ACG8V1_000774"
     CDS             complement(35925..37145)
                     /locus_tag="ACG8V1_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0328890.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(37426..38271)
                     /locus_tag="ACG8V1_000775"
     CDS             complement(37426..38271)
                     /locus_tag="ACG8V1_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0328891.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        11418 a         7973 c         6725 g        12444 t
ORIGIN      
        1 aaaataaaca tgcttttttt caactttaag aaaatttcaa ataaaaaagc agtttttctt
       61 tgctgattct actatggtag aaaaaaactg cagacaaacg aacgtctgtc tacagcagta
      121 tttcatgaat ttttgtcaaa ctcgatctaa atgccatcct catagaaacg accaagtatc
      181 tggacgtttt ctgcaatact tacaaaagct tgtggatcag aagctttcat tgcttctcta
      241 agtagaggca gttcgtatcg tgtaacgatc gtcatcaata ctgtctgctt atcatgctta
      301 taagcacctt ctgcttcatt gatgatcgtg atccctctac gcatacagtt ttggatttca
      361 tttactactt tttctggatg tttcgttacg atcattacct gcatcttctt ttgtttagta
      421 taaacgacat cagttacttt tccactgacg aaaatagaca acgtactgta aaacatatat
      481 tgccagccaa atagaattcc ggcaacaaaa acgatcaatg cattgaagta gatcgaaata
      541 gaaccaatcg actttcctgt ttttttacga atcgtgatac tcacgatatc tagaccgccc
      601 gatgataggc cgtttctcaa cgctagccca atacctagtc ccatcacagc ccccccaaac
      661 aacgcacaga tgattggatc ggtagatagg actgtttccg gtaaaatatg gatacaaagg
      721 gaggtcaaag tgactgtgat aaaagtgaaa attgtaaact tcttcccgat ttttaaccac
      781 gccaatacaa acaaaggtat attcagtaaa tacaaagttg ttgataccgg tacttcgaat
      841 cctatcagtg attttgacaa agtcgtcaaa atctgagcta gaccagtaat accacttgaa
      901 taaatatgtc ctggctgata aaagaaattc aatgcgactg atgcgatcaa cgcataaacg
      961 atagataccg aaagcttggt cgtgtaatca tgataagccc aattttcgaa tgtttttttc
     1021 atggaaaagc cccgctttat atgttatggc ttcattataa agtgtttttc aattaaatca
     1081 aggttgtcaa aagaagcgta ttcgacaaat cctttttacc tttttatcgg ctattcttct
     1141 atattcgtta catcaatatt caattcgaac aattgtaaag gcgaaaccat acttggtgca
     1201 tcactcatca catctgctgc ttttccgttt ttaggaaagg caatgacttc acgaatatta
     1261 tcttctccgg ctaataacat tgcaaaacgg tccaatccta atgcaatacc gccatgtgga
     1321 gggaatccgt aatctaaagc tgttaacaag aaaccaaatt gttcttccat ttgttctttt
     1381 gtaaagccta gagtttctag tactttttct tgaagctcac gagtatgaat acggatagaa
     1441 cctccgccta attcataacc gttcaagacg atatcataag cttctgcata gacttttgca
     1501 gggtcttcag ccaataacgg gatatcttct tcttttggca tcgtgaatgg atggtgagca
     1561 gagacatatc gaccttcttc ttctgaaaat tcaaattgtg gccaatcagt tacccataag
     1621 aaattgaatt tcgattcatc aattagtcct aattcttttc ctaaacgtgt ccggatagca
     1681 cctaatgcag cagcgacgat ttcactttta tctgcgccaa acatcagcaa gtcacctggt
     1741 tttgcatctg ttgctttaat gattgcttct gttgcttctc ccatgaattt agcaattgga
     1801 cctttcaatc cgtcttcttc tactttcagc catgcaagcc ccttagctcc gaattgtcct
     1861 acatattgac ctaactgatc catgtctttt cgtgagtagc gatctgcagc gcctttagca
     1921 ttcaatgctt tcacgacacc gccgttttca agagccattt ggaagacttt gaattctact
     1981 tctttgactg tatcgcttag atcgatcaat tccatatcaa aacgtgtatc cggtttgtcg
     2041 ctgccgtagc gagccattgc ttcatcatag gtcatacgag gaaatggcaa tgtgacttcg
     2101 ataccgcgaa cttctttcat taccttcgca atcaatccct ctgtgtaagt ttggatttct
     2161 tctgctgtta agaacgttgt ttcaatatct acttgcgtaa attcaggctg acggtctcca
     2221 cgtaaatctt catcgcggaa acaacgaacg atttgataat aacggtcgaa tccagcattc
     2281 atcagcaatt gtttaaacaa ttgaggagat tgcggcaacg catagaaatg tccagcatgg
     2341 acacgtgaag gaactaggta gtctcttgcg ccttctggtg tagatttccc taggtaaggt
     2401 gtttcgatat cgagaaagtc attatcatct aaataatgac gaatcgcttt tgttgtcgca
     2461 tttcttaatt tgatgttttt agtcattttt ggacgacgca gatccaaata acgatatttc
     2521 aaacgtattt cgtcactgat tgcttgatca tcttcaattt gaaatggcgg ggtttttgct
     2581 tgattcaaaa tcacgatttc acttgcaatc acttcgaatt caccagtggc cattttagga
     2641 tttactgcat tttctccgcg tcttctaact tccccagtca cttcgatcac gtattcactg
     2701 cgacatttat ccgcgatttc ccatgcttct ttgttgattt caggattaaa gacgatttga
     2761 acgattcctt cacgatcacg taggtcgata aagattactc ccccaaggtc tcgtcttttt
     2821 tgtacccatc cttttaatgt aactgtttgt tctaattgtt gttcggaaac taatccgcaa
     2881 tagatcgttc tttgtgccat ttattttttt cctcctactc gaataaagtc atttcatcat
     2941 agacttcatc aaaatgttca tatatctctt ctaatggaaa acttttttct tttcggcttg
     3001 ccattgcttt gacattaatc gtttgactag caagctcatc ttcaccgatc accaatacta
     3061 atttggcatt cgctttatct gctgtcttaa attgcgcttt tgctttacgg ttcatgaagt
     3121 cgcggtcagc tgaaaaacca aagtttcgaa tcgcttggac cactttcaat gcttcgatat
     3181 ttgtttcgtc tccgagtgcg accacgtaag catccaactc attaaatgca gggataacta
     3241 cttcctctgc ctccatagtc aagagtaatc gctcgatacc cattgcaaat ccgaagccag
     3301 gtgttgaagg accacctagt tcttccacta gattgtcata acgtccgcct gcgcaaatcg
     3361 ttgcttgtgc ccccattttt ggtgcgtcac tcatgatttc aaaaatcgta tgtgtgtaat
     3421 aatctagtcc gcgaaccata ttactgtcga tttcataagg aacagccaat gcatccagca
     3481 tggaaacaac ggtatcaaaa tgtttttgag caggctcgct tagataatct aagatagatg
     3541 gagcttctgc cacaaatttt tgatctcgtt tgtctttact gtccaatacg cgtaatggat
     3601 tttcatgaag gcggcgttta gagtcttcac tcagctctga ttcaaaaggt aaaagatagt
     3661 cgatcaatgc ttgacgatag gcttgacgcg tttctttatc acctaatgaa ttgatgacta
     3721 gccggatttg cgtgatcccc aactgtttga agaaatcaag tgccatcacc atgctctcca
     3781 catcagttgc agggttttct gatccaaatg cttctactcc gatttgatgg aattgacgta
     3841 accgtccttt ttgagggcgt tcgtagcgaa acattggtcc catataaaaa gttttgaacg
     3901 gtttcgcata ctctgggccg aacaatttat gctcaacaaa agaacgaacg attggtgctg
     3961 taccttctgg tcgaagcgtc acatggcgtt cccctttgtc ataaaaatca tacatctctt
     4021 ttgaaacgat atccgttgta tcccctacac ttcgggatat cacttcatag tgttcaaaaa
     4081 tcggtgtacg catttcatta tactgataat ctctgaataa taaacgtgct gtttcttcga
     4141 caaactgcca tttttctgat tcgcctggca aaatatcatt tgttcctttt ggtctttgga
     4201 aactcatagt tttctctccc tttcttagct atgtaaataa aaaaatcacc cttgttcatc
     4261 atgaacaagg gtgaatgaaa ttcatacacg gtaccaccta attttgaaag atttctcttt
     4321 cctcttacga ataacgctcg tacgcgggac ggcttttcac tcgtccatct ccagagtgtc
     4381 tttaggctaa tgaaacttta gatcattttc agcctcgatg atcctctctt gtaaagtttc
     4441 aaaaacttac tcgctctttc attgacattc gatattactt ataacctact aaaaaccagt
     4501 ctcaaagtca agagatatct gactatttcc cctcacaaaa acgttttagt tcagcttttt
     4561 ggtattttgt tttagactag acaaaaagga ggaatcagct atggcagaaa taagaatcgg
     4621 acattcacaa gtctacgcag aacagcttgg attaggagca aatgccgttg gaggacataa
     4681 tctttttgac ggtttggaag atgagacagg aaaacaagta gttcgtacag ctttgaatag
     4741 cgggatcaat ttgatcgata ccgcttatgc ttacggaaac ggccgttccg aagaactgat
     4801 tggcgaggta ctgaaggaaa aagaatatga tcgctcacgt gtcgtcatcg ctacaaaagc
     4861 tgctcatgta ccaaataaag ggcgaacatt tgataattct cctgagtttc tcaaacaatc
     4921 ggtggaagat gcactaaaac gtctgcaaac tgattacatc gacatttttt atatccactt
     4981 tccggatgaa agcacaccaa aaaatgaatc agttgctaca ttgcacgaat taaaagaagc
     5041 aggaaaaatc cgagcagtcg gtgtgtcaaa cttcacgtta gaacaattga aagaagcgaa
     5101 tgcagatgga tatgtcgatg tagtagaaga taaatacagt ttgatccatc ggcaggctga
     5161 aaaagaatta ttcccttatc ttgaaaaaaa caaaatcagt tttgttccct attttcctct
     5221 tgcttccggt ttgctgacag gaaaatatga acttggtgag gaaaaacaat ttggtgaagg
     5281 agatcctcga aaaagaaatc cagatttcca aggagaacgt ttcagagaaa ttttaaccgc
     5341 tgtcgatgta cttcgcccca ttgctaaaag ataccaagcc actcctgcgc aacttgtttt
     5401 agcttggtac atgaagaatc cacgagtgtc tgtagttata cctggagcta agcgtccaga
     5461 gcaagtatct gacaatgtcc aagctttaga tttgcattta tccaatgaag attatcaaac
     5521 cattgatgaa gcatttcgtg gattttgatc aattgacctt atcaatttat agtaatatga
     5581 aaaaaggaag atcagcactg tttgatcttc ctttttttcg ataagtttat gctaatctaa
     5641 ttcttgtaaa gtagcgctcg gctattcgga taagtcggaa actgattgaa tggaatcgtc
     5701 caatcttgtt caggtacaga gaactcaaac ttatttacta gctgatcgga aaacacacgt
     5761 agactatgaa gagtgatcca ttctcctgca cagcgatgca tttgtcgaaa atttcccccg
     5821 ccttgtgcaa tcatctcata ttcttcttta taggaaatat cttttgcttt ccctacatac
     5881 cgtttgatca taaaggaatc tggcgcttca actgttcgtt catcatggtc tgttccatat
     5941 aaatctaaaa taacccaact gccttctggt atacgataac catctacttc tacttctttc
     6001 agcgaaatcg caggtagcat tgggaagaaa ggataatagc gtctcatttc ttgaataaac
     6061 ggatcttgta acgtcgaaaa gtcatttttt aattgctgat acaaatctgg tctactgaaa
     6121 agtgcatgtc ccatcaatgc cgcccacaca gttagagcaa ccgttggacg gatgatgttc
     6181 aataattcaa cagctgccac ttccagaggt aaaagctgac catctaaatc tgtggcattg
     6241 gcaaaagcat ataatgccac attttctttt ccagccacag gattttgtcg agcttcttca
     6301 atcaagcctt gcgcccattt ttctgatttt ttgcgatttt cgacaccttt tatgtgatca
     6361 atcggagaag taaacgttcc gctgatcata gagatttgat attctgctaa tttttcgacc
     6421 tcatctttag aaatcgctga taaattgata cctgcccact cgcaaataga tgtaaagaaa
     6481 accatcttac ttaaatcaaa tagttcaaat tgaccatgct gtgcttcaag tgcttgtgtc
     6541 agattcttat ctaagatacg atgataatct tccattcttt ccggtgtcat tagatccata
     6601 aaaatatttt tccgatggtg atgagcagct ccatccaaag tctgtactcc accttgacca
     6661 aaaagtgtct tcaatactaa ttttggcata gcaccttttc gtttgaaatt ccttggatca
     6721 taaaatactt ttgctgctga tgatccgtag atcgtgatag cttctttatt gaaaatcttt
     6781 gctttgacga cgggagcgtc cgcttcatgg cgtaactcct ccaacatgtt gtatcctttt
     6841 tgatacaatt tttttaaatc tgtgattttg atatctacta ctggtacttc tttcatcttc
     6901 agcaacctcc tataaaaaat aacttgtata atttaagctt aacttttttt ccgactgctg
     6961 acaaaagaag cgctttttgc agacaaaaaa gccacttaag ttagagttta tcagtctaac
     7021 ttaagcgacg tgtttcatag tttttactag tgaaaaccag attactttat ttgtttttag
     7081 aatcaataat gatcgttaca ggtccatcat tgacgagaga aaccgacata tctgctccga
     7141 attgtccggt agcgactgtg atgccgcgac ttctgattcc ttcattgaat gattcgtata
     7201 aaggaatagc tgtttctggt cgtgcggctt ccacaaagct tggtcgattt ccttttttcg
     7261 tatccgcaaa cagtgtgaat tgagagatac ttaaaatttg tcctcccaca tcttctacac
     7321 taatattcat ttttccttgt tcatcttcaa aaatacgcat ttgtgcaatt ttttttacca
     7381 gatagtccac atctttttgc gtatcggttt catgaatgcc taataaaaca agtaggccgt
     7441 agccaatttt tccaatttct tgctgatcga ctgacacagc agctttactt acccgttgaa
     7501 caactgctct catatctgtc ctcctatcca ttcgtccggc gtacactgta gacatcaggt
     7561 actgctttga ttttatccac gatactcttt aggtgggata aattctggat accaaccgtc
     7621 aaatggatcg tcgccatttt atctttattc gtccgtgctt caacgctgtt gagatttttc
     7681 gttagcgcat tgactgtttg aagtacatca ttcaataagc ctgaacgatt atagccgtaa
     7741 atttccaaat ctgcatcata ctcttttcgt gtattcgatg tatcttccca ttcgacttca
     7801 atcaaacgtt ctgctacatt tggtttgtca ggctgaacat tcggacaatc tcgacgatga
     7861 atggatatcc ctctgccttt agtgatataa ccaacgatat catcaccagg aatcggatta
     7921 cagcagcgac tgatacgaat tagtaagttt tctacccctt gaatgacaac gccaccttca
     7981 tgacgtactt tcattttgtc aggttctttt ttcggctgat tcatgatttc ttctgcttct
     8041 tgcttttgct gctcgatttt ttgttcttta cgttcttttt cagtcagacg attggccatc
     8101 gtcaaggggc taacttctcc ataccctaca gctgcataga gatcatcttc tgtttgataa
     8161 ttaaaacgat cgagtgcttc ttgcagtttg ttcttcgtca aaatatcttt aggcgtaaat
     8221 cctagatctg taatacattt gaccacggat tcgtggcctt tgatcacatt ttcttctcga
     8281 tcttgggctt tgaagaaacg tttgatctta tttcttgctt tgctagtagc aactaatttc
     8341 aaccagtcgc gacttgggcc aaatgaattt ggagaagtca tgatctcaat gatatctccg
     8401 tttttcaatt tgtaatcaag ctgcaccatt ttgccattta cttttgcacc agtggttttg
     8461 ttaccgatat ctgtatggat actgtatgca aagtccaatg gtccggatcc ttttggtaac
     8521 tcagtaacgt ctccttttgg tgtgaagacg tagactttat cactaaagat atctcctttt
     8581 acgccttcca taaattcaga agcatcatag ctttcgtctt gaagttcgag tatctcatgg
     8641 aaccagctta attgtttcgt cataccatct ggttctactt tttcattttt tccttcttta
     8701 taggcccagt gtgcagcaac cccgaattca gcgatttcat gcatttcctg tgttcggatt
     8761 tgaatttcta ccggatttcc tgccggaccg atcacggtag tatgcaaaga ttggtacata
     8821 ttagcttttg gcatggcaat ataatcctta aatctgccgg gcattggttt ccattttgtg
     8881 tggattgctc ctaatacagc ataacaatcc ttgattgaat ctacgatcac tcgaatagct
     8941 agcaagtcat aaatttcgtt gaactgcttt ttctgatctt tcattttacg atagattgaa
     9001 taaatgtgtt ttggccgtcc atagatttct gcaaaaatcc ccagctcttc tgtcgctatt
     9061 cgaatatctt caacagtacc acttacgtat ttttcacgtt cttctctctt cgtctgcatc
     9121 aagtggacga tacggtaata ctgttttgga tttagataac gaagtgctgt atcttctaat
     9181 tcccatttga tccggctgat ccctagacga tgtgcaagag gcgcatagat ttctaaagtt
     9241 tcctgagcga tccttctttg cttatcttct cgcaggtgct tcaacgtacg catgttatgt
     9301 agtctatcag ctaatttgac catgataact cgtaaatctt gagccattgc tagcagcatc
     9361 tttcggtggt tttctgctag ctgctcttcg tgggatttat attttatttt ccctaatttg
     9421 gttacgccgt ccactaacat agcaacgtca tcgccaaatt cttctctcag atcttctaaa
     9481 gtgatttctg tatcttcaac aacatcatgt aagaagcctg tagccactgt atggggatcc
     9541 atttttaaat ctgctaatat acctgcgact tggataggat gaataatgta gggttcgcct
     9601 gattttctaa actgtccgtc atgtgcagca gtcgcgtatt cgcatgcttt ctcaacaaat
     9661 gctacatgtt cgggacccat gtattgacta acgagcttta tgacaccggg tcccgttaaa
     9721 atttcttctt ttgtcacagg aatcgatccc cctttctttt ttcggtaaaa cgggaatcgg
     9781 gacaaatatc tgtttgataa ttgtcccaaa atccgtgcag ttatacgttt ttttacggaa
     9841 atgttcgctt tttaaaagct ttttcaaacg atttttcgta tccattgtat aaataattgt
     9901 ttctattata caaattgttg actatttttt caattctttc tgtcgatact cttttattaa
     9961 tgaagaacgt tagctgaaag aaggattcat taattcccat tgatagctag cagcacttaa
    10021 taaataaaga ggtgctgttt ctgtacgaag gattcttggt cctaatccac aaagtatccc
    10081 tttgtgttct acaaaataat cgacttcatc gcgggataat ccaccttctg gtccaaagac
    10141 agctaagatt cgactatctg gttttgtttc actaagaatt ttggctgcat ttgatttttc
    10201 gccttgcttt gctgattcct cataagcaac caagaccacg tcataatcat ctagccgctc
    10261 aaataaatct tgtttcgttt ccaataactc tactgatggc acgatctgcc gatgggattg
    10321 ttccgctgcc tcttgagcaa tcttttcaag ccgttgttgt cgtttcatac gtttttttgc
    10381 atcccattta acgacagagg ctgatgccgg gaaaccaaga aaagcttgcg ctcctaattc
    10441 agtggatttc tgaacgatcc aatccagctt ttccccttta ggatagccac tcgctatggt
    10501 aatcgaactt ggcagttcct tttgattttc ttctttttta atttctttca gataaacagc
    10561 ctcttcagta atctccgtga tttctgcaac aatggctatt tgatcattga atgccaaaaa
    10621 aacttcatcc tcgggttcca tcctcataac ccgtacaatg tggtgatagt tttccccttc
    10681 taaaacaaaa tagtcttttg attcatatga ttcttttaaa aaataccgct gcatctattc
    10741 cacctctggt tttttcaaaa taattgcata ccagtctttt tgctggaaaa tttgatccac
    10801 gataaaccct tgttctaaca tggcatccag aaccatttgc ttcttctctt caatgattcc
    10861 tgaaacgatc agcgtgcctg tttgcttcag taaacgccaa gcatctggga tcatcaataa
    10921 gatgatatct gctaaaatgt tcgcaacgat tacatcactt tcaatctcga ttcctgtcaa
    10981 aagatcgtta gctgatacat ggacatcttt ggctatttca ttcatatcca tattttcttt
    11041 cgccgaacga acggctactt cgtctaaatc aaaggcatag acatcttttg caccaagata
    11101 ttttgctgca atacttagca cgcccgaccc agttcccaca tctagcaatg tttcaccacc
    11161 acgcaagact gtttctagtg ctgtcaatgt caaggaagtc gtgggatgag tgcctgtgcc
    11221 aaatgccata ccaggatcta gtgtgataat tttttctaga ggatcttctg tctggtagtt
    11281 ttcccagctt ggcgcaaccg tcagcattcg cgtaatacgc accggatgat agtatttctt
    11341 ccaggctgtt gcccagttgc tttcctctac ttcactaact gttactttat tttttccaat
    11401 agctagacca aactcaggta attgtgtgat acgatctttg ataaaaggca agatttccgg
    11461 taagaaagtc gtttccggaa aataagccat gactagtgcg ccttctttca gcgatgtaaa
    11521 atcattttta tctaaaatct caccatattg atccgactga aaatgttcaa catctaaaga
    11581 atcttcaatc gcgacaccac tcgcccctgc ctccatcaaa atattagcaa ctgcttcgac
    11641 tgcttcactt tcagtttcta cttttacttc attccatttc atatctgtca ctccttaata
    11701 acttgggtac ggtaaataag tatctgcttc attttgctgt tttacttcta ctaattctgc
    11761 tagttgctga ttatcccagt gaagctcgaa tacagcttcg tcatcgtctt catccaaaaa
    11821 atcaagcaaa tcgctttgcc cttgagctaa tacttcattt agatattcca ctaaagaatc
    11881 agctactgat tttgccaacc ctttttttcc ttcataaggg attactgcta gataattgcg
    11941 ttcatcaaaa acagatttct gaggattaaa aagaagtacg ccgtcttcaa attcgataat
    12001 tggttcttgc gaaacgatcc cttctgcatc atcaatctca aattgtgctt tattttctgc
    12061 aaacaagcga acaacgatct cgatcgtatg attttttgta tcccaatcta aagctacatc
    12121 atagtctgtc acttgttttt caattatttc atctaaatat gttaatagat ccttcttttt
    12181 cattttcttt cctccaaccc caatctttct cgatttggtt ttttctttgt aaagagatat
    12241 aaggatttcc cgacacaaca aaacgcaacg gcttttctgt ccatacacct ttgtttggga
    12301 taccgattct aggcaaagca ttgatctttt ttggtgtctt ttttttctca aaaactaagc
    12361 gtaacgaact atctgctata gactgaccat aaagttcttg aggaattgct aatgcttcca
    12421 ctaattttcc cggaccgttg ctgatatctg gaccagtttt tccgccacga tttttactca
    12481 tttgatcaat cattgcggcg ggttcgattg cacgtatcat tactccttga ggaatccctt
    12541 ctggctgagt aatgatgttc aaaatccgat gagtatgcat cgtgtaaagg tagatcgtac
    12601 ctggtttttc atacattgcc cgcacacgcg gtgttcttct taaaccatag ctatgggcgg
    12661 cctcgtcttc tggacctagg tatgcttccg cgtctacaat atatccagct aatcttcctt
    12721 ctggtgtgtc atgctccaaa tacatcccca ataaatcccg tgccacttct ggcgtcgttt
    12781 tattccaaaa aaattcatct atcttttgtt tatccgtcat ttttctcacc tactttatca
    12841 tacacaatat tagttggata agctatgaaa acaaaacaac agaaggctgt tctttctagg
    12901 catcttgcta aatatgatat aattaaggga cgtaaaaaca agaaggagga aaatcctatg
    12961 caacagccat tggcttatcg catgagacct cgaacgatcg atgaagtcgt tggtcagcag
    13021 catctagtcg gggaaggaaa aatcatacgc cgcatggtcg atgctcgcat gctctcttcc
    13081 atgattctat atggacctcc tggtactgga aaaacgagta tcgcaagtgc gatcgccggc
    13141 tctactaatt atgcatttcg catgctaaac gccgctacag attcaaaaaa ggatctacaa
    13201 gttgtagcgg aagaagcaaa aatgagtggt acagtgatcc tcttattgga tgaagtacac
    13261 cgattagata aaacaaaaca ggattttctg cttcctcatt tagaaagtgg ccgaatcatc
    13321 ttgatcggag cgactactga gaatccttat attaccatca atccagcgat ccgcagccgt
    13381 acacagatct ttgaagtcaa accattaaca gaacaagaca tccagttggc agtcgaacat
    13441 gctttaaggg ataaagaacg cggccttgga caacaagcaa tccagttaga cgaagatgct
    13501 cttcttcacc tttcgcgtgc aacgaacggc gatttgcgga gtgcattgaa tgggctggaa
    13561 ctggctacac tatcgactcc ttcagataaa gaaggaagga ttcatcttac actgtcgatc
    13621 attgaagaat gtgtccaaag aaaagctctt acccatgaca aaaatggaga tgctcattat
    13681 gacgttattt cggcttttca aaaatcgatt cgtggaagtg atgtggatgc tgcacttcat
    13741 tatctcgccc gtttagttga ggcaggtgat ctggcaagta tttgccggcg tctgatggtt
    13801 atcggttatg aagatatagg tttaggtaat cctgctgctg cggctcgtac agtgaacgct
    13861 gtattagctg ctgaaaaatt gggattgcct gaggcacgta tcccactagc agacgtcgtc
    13921 gtcgatcttt gcttgtcgcc aaaatccaat tctgcctaca tggcattaga tgcagcactt
    13981 gctgatattc gagagggcaa agctggagat gttcctgatc atttgagaga tagccattac
    14041 aaaggtgcga aatcactcaa ccgtggcgtg ggttatcagt atcctcatca ttttgatcag
    14101 gcttgggtca atcagcaata tttaccagat aaactgaaga atgctcagta ttatcagcca
    14161 aaagatacag ggaaatatga acaggctctg ggtcaacagt attaccgtat aaaagagtgg
    14221 aaaaagcatc ctccaaaaaa ctagacaaga aaacggttct gtttctgcga gctctctcat
    14281 tgctcttttt aaattcatat gctactatag aggtgggaaa tctgtgatgt tcgaattgtc
    14341 ctctttgtgt gttgaccgaa catttttatt gatatcttgg gacccgtctt gtgtcactgc
    14401 tggtaacatg ccttatcatt tggtagttcg aagctacgac catgggaccc acctgcttga
    14461 attcgcggga tcaatactac aggacaaaca acggcattaa cggagattac catcttttaa
    14521 agaataaagc aatttgctct tggcaaattg ctttttctat ttgttgctta ttttggagga
    14581 tttgttcatg attaggattt taatgattat cgccacttta gtattattat tcgttagcta
    14641 ttatttattt aataaacagg atatcttttt tgtattgatc aacaaaaatg ataaaaatca
    14701 aggattccta caattttacg gagctgcata tgctgtttta ggcgtaatgg gtattttaac
    14761 agcttttttc aatcaacgct ttattgcctt ggtcttttta ctgatcgtaa ttattgtttc
    14821 tgccaccttc agtatccgat ttgcaaaaaa aattgctgaa ccaaagcagt aaataattct
    14881 ctttttccct aaagtgttct acaataaaag agtacaatac gattgacttg aggagtgaat
    14941 gcttatgtta caacaataca aaaaaatcat ggttgccgta gatggttctg atgaagctga
    15001 attagctttt aaaaaggcgg tcaatgtagc tatccgaaat aacggagaac ttctattagc
    15061 acatgtcatt gacacacgtt ctttccaaac cgtttcatcc tttgatggaa tgcttgcaga
    15121 acaagctact gaaatggcaa agcaaacatt ggcagattat gaaagcaatg cgaagaaagc
    15181 tggcttgaat aatgtaacct ctgtcgtaga atatggctca ccaaaacaaa ttattgccag
    15241 agaaatccca gaagataatc aagtagattt gattatgtta ggagccacag gattgaatgc
    15301 tgtagaacgt ttatttattg gttctgtttc tgaatatgtt attagaaacg cagcttgcga
    15361 tgtgcttgtt gtccgaactg atttagaaaa ccaattgcca gctaaagacc acaccaaagc
    15421 ttaacacgta gacgacctgc caactatcgg caggtttttt tgcacaaaaa accgtgataa
    15481 aaacaaaaga cgcggcaagc actcatcggt ctttataatg tttaaaataa agggaaaaca
    15541 gtcaatgagt tcaagtaaaa taaaaaaaag tgaacaccag ggcgattagt cgccctggtg
    15601 tggtttaaaa cgacctaagc atgccaaagc ttattggttc gtcaatgtgg aagtttccga
    15661 tgaatctgat acgaaaaaat atgaagtatc gatggcggat accgatttta tcgccaaact
    15721 aaaattttat ttcgggttcg ggtaccgcga gaaggcaagt gctacattcg gtaaatcatt
    15781 caagaattga tgaaaggcta ccgcttgcca ttgcaaccac aacgatactc ctgaacgcga
    15841 tgttggtgag atttccaaag agaagagctg tttaatgcaa cggtattaag caaatggcaa
    15901 aaggaagtca tgtagacaaa gttttttatc aaacaccata tgatttcgcc tgaacgctgg
    15961 tttagctcag aatacaatga tgtggtccac gagacattac cctcagtcag ataaaaaaag
    16021 ctctctgaca gaattacaca tcgataaata ttaagtaaat gtcagcgcta gttgaacttt
    16081 ctgacacttt caggtacact ggaaaagaca actacacgaa ggagggaaaa tactatgatg
    16141 cgcatgacta aaataacaag taatgaaaat taccaacgat accatatcgc atttagccat
    16201 gcaattggtt ttacagttgc tgttgacggg acaagtttgt ccaaaacagt tgaattcacg
    16261 taaaatcaat ggccatctca aggtattttt ctcatgataa tctgagttta aataacacag
    16321 gccatggata accatggcct ttttgagtgg tctcaaggag agagatcatg atcatacaag
    16381 caatttctat taaaaaaaca atcggcggga atccattatt tgaggattta tcctgtcaaa
    16441 ttgaagctgg tgaaaaaatt gccttagtcg gtatcaacgg aactggcaaa tcaacctttt
    16501 tacaaatctt gaccggcaga gaaggtgtcg acagcggtgt tatcagtcgc aaaaaaggct
    16561 taaagatcgg gacggtcgaa caagaactta cagtttccga agcgaccgtc aaccactact
    16621 tgctccattc tgccgctgag atacaggacc tcaaacagca aatgagtcgc tacgaagcat
    16681 tgatgacaga accagataca aacttagaga agtgtctggc ccgttacggt gagctgcagc
    16741 atcgctttga agaactaggc ggttacgtat tagaagaccg gattgcagcg atcttgcaag
    16801 gcttagggat tccacaccgt caggaagcac ggttaacaga acttagcgga ggcgaacgag
    16861 ttaaagtcgc tttagccaaa attttagcag ccgatgccga tttactttta ctcgatgaac
    16921 cgaccaatca tttagatctt aacagtattc gctggttgga aaactattta cagaatacaa
    16981 aaaaatcggt tctggtcgtt tctcatgatc ggcaatttct cgatcaagtc accacaaaaa
    17041 catgggaact ggaagaaggt gcattgattg agtatcccgg aaactacagc cgttttcgag
    17101 tcttgaaaga agctcgcttg gctgagctga caaaaaatta cgaactgcag caaaaagaag
    17161 tccagcggtt gaaagtcatg attcgtcgtt ttcgtcagtg ggcacatgaa ggagacaatg
    17221 agtccttctt caaaaaagcc aaagaactgg aacgccgcct ggcgaaactg acactcgtca
    17281 aaccgccacc tcccccgaaa aatcggctgc aaagcttgtc taatggtgga aaatctggca
    17341 aagaagtttt catcattcaa aacttgcacc agcagtacgc tgatcaagta ctattcaaag
    17401 acagcagttt tgcagtctat cgtggagatc atttagccat catcggcgac aatggtgcag
    17461 gaaaatcaac cttactcaaa ttattactcg gtgaggaaaa gccttacggt ggcaccatca
    17521 aattaggtag tagcttgcaa atcggttatt tgccgcagca gctgcagttt gccgatcccg
    17581 atagccgttt gctggcttac gccatcacat tgactggaaa cgaagaagtc gctcgacggc
    17641 aattagccaa aagtggcttt tatcaaagcg atgtgggtaa acggatcaaa gatttatccg
    17701 gtggtgaaaa aatccgtttg gcgttgctga aactcttttt agaaaaaatc aacgtcctga
    17761 ttttggatga accaaccaat cacctcgaca gctatgctcg agaagaaatt gaagaaatgc
    17821 tgcaagacta tcaaggaacg ctcctagcag tcagccatga ccgctacttt ttgcagcaac
    17881 acttccaaga agcactcgtt attgatcagg agcagatcag ccgccagccg ctcggccaac
    17941 tgattgacta ggacgttttt tcgcaacaaa aaacttgatt ttcatacctg tgctttcacg
    18001 attcaggcaa agtacaggtg gaaaatcaag tttttattat tcgatcaatg aatgtttcgg
    18061 atcggttttc tagctaaaca taaagcgaag aataaagtcg acagcccacg aggctgatct
    18121 aaaaatgttt tttcttttta catatgtaat gtcccttata aagatgaatg aaaataaaat
    18181 attgggattt tcctgaaaat caataaataa aatgccagtc ttttcccttt tgaccacttt
    18241 tccaagcaaa attccctaag aagatcctgt aaaaaagaag ggaaattgaa gttaaattac
    18301 cattttgcgg tcttctccgc aaaaaaaaca catgcaagaa ttccctcttg catgcgtttc
    18361 atcttttatc ctaacgaatg ctttggctta gccgcgcaga cgctccacat cacgggcaat
    18421 cattagttcc tcatctgttg ggatcactaa tactcttact tttgattctt ttgtagagat
    18481 atctacttct tcaccacgca attcattcaa cgctggatcg atttcacaac caaaccaaga
    18541 catacctttg atgatttcac tacgtacatg tccgtcattc tcgccgatac cagcagtaaa
    18601 gacaattgca tcaacaccat tcatcgttgt cacataaccg ccgatgtatt ttcggatgcg
    18661 gtccacgaaa atgtcataag ctaaacgaat atgttctttt tcatagttct tttcaagatc
    18721 acgcatatca ctagacaatc ctgttaggcc tagcagacca gatttcttgt tcaaaacatt
    18781 gatcatttca tcaatatcaa tctctaattt ttccattaaa tatgggagaa cagctggatc
    18841 gatatcacct gaacgtgtac ccattgtcac cccagctaat ggtgtgaatc ccatagaagt
    18901 atccatggat ttacctccat cgactgctgt gatcgataca ccatttccta agtggcaagt
    18961 gatgattttt agttcttcga tcggtttgtt caataatttt gccgcacgtt ccgacacata
    19021 acgatgactt gtgccatgga agccatattt tcttactttg aaatcttcat aataagacac
    19081 cggtaagcta tataaataat tttgctttgg cattgttgag tggaaagatg tatcgaatac
    19141 agccacactc aaaatgtcag gtaaaatctt tttaaacgct ttgatcccca ttaagtttgc
    19201 aggattatgc aaaggagcaa attctgctaa ttcagcaatg cggtccatta ctttctcatc
    19261 gatcactact gaatccccat aagtttctcc tccgtgaaca acacggtgcc ctactccggt
    19321 gatttcatca taagagctta aaatttgcag ttcaatcaac tgatctaaca gcatttttac
    19381 tgctacttcg tggttttcga tatccatgat ttgttcgaat ttttgattat cgccgtactt
    19441 gatggtaaaa atagaatctt gtaaaccgat acgttcgacg atccctttcg caactacggt
    19501 ttcttctggc atttgatata attgccattt caaacttgaa ctcccagcat tgattgcaat
    19561 tgttttagac atatgactct ccttctcgtt gaatgattac agagcagatg atttccagtt
    19621 tttaaactcg ttgaaaaatt ccgtcacctt ctctggttct tttaatgaag caagtttcac
    19681 aagcaagact tcttttactt gttgtgcttg tggtcctttt ttctgtaaga tcaaaatact
    19741 tttttgcgat tgtttattac ggaacaattc atctggcaat tgaatcattc cttgaagata
    19801 accttcttct ttaaaccatt ttttgatttc ttcactttga tccgtttcta aaaatccact
    19861 cggcatcaaa aacaaaccaa aaccatccgg cttcacatat ttcattgatt gctctagcaa
    19921 taaatgatgt gcataactat ggccttcttc ggtacttgtt aaaaactctt ttgctttttc
    19981 gtcattcgga taataaccga ttggcaaatc gctgattgca aaatcgacag gatctattaa
    20041 taattcctgc aacccgtctt gatgaaagta ttgaacattt gcttgtgtca aatcacttgt
    20101 agaagctgcg acagccaaca atgtatcatc aatatccaca ccaattcctt ctgtttggta
    20161 ccccgcattc gataggttta aaagaactgt taataacaag ttgcccattc ctgccgcaat
    20221 atccagaatc ttaaccggtg cttttttatt tgtgtatagt tgttcaataa gaaaaacaaa
    20281 taaaaaaccg atactatcag gagtgagttg atggttcgcc tgcaaatgtt ctgtctggct
    20341 gcccttcaat aaaagtaatt gagacagtct gcgccattct tctggctcga aagaaagttt
    20401 cttcaactct tcataaagag ctgtgatccg ttgcgttgtc tcttttgtcg gcactccatc
    20461 caccacacgt acttgataat catctattaa attttctgca ttttcgacat aggcttctaa
    20521 aaaggaagtt cccaatgcat tctgtaaaag ctgaatagct tccaggtttt gattaaaccc
    20581 ttgttccatt ttttctggta acatggtgca cctcatttct agatatatta taacaatttt
    20641 ttcacctgtt taattctatc gaacaattga caaaatttca atcgttttca ctgtttttta
    20701 tttgtgaaac ggcaaacatt tttttattaa aaacagtgtt ataacagaaa agagggcgtt
    20761 ttcacgaaca ttccatcatt ctgtactagt atctgtatca ttttttactt cttctgtgaa
    20821 ccgaaagata aacggtgaga cttccacctc taagaatagg gactgttgta cccactgata
    20881 tgttacagat ccagttgtgt agtaaaaaac tccttccgaa ctttcggcaa gcgtagactg
    20941 ttccgataaa gccatctgct tcattatttt tgctatataa taatttttga ttcgtatatt
    21001 aagatccatt gacaaacggt agctttctaa tgctgccatc aataaaaacg tacataaaag
    21061 aaataaaagc aagacagaaa ataaaataga gcccttataa cattttctac tgatcgtttt
    21121 ttgtccatat ggcatatcca tcttctccat tttcaaagtg tacataaaaa gaaattgtgt
    21181 gttctttttc caataattga agctcagtga tacttgttaa cataggttga tgtccgccgg
    21241 aagttcttct tttacgtatc attgcctgat atctttcgat gatatactgt ttatcctcga
    21301 tcgtgtagtg cagttgctga tttttcacag agacattgtg actgctcttg agttcttctt
    21361 ctaattgtgc taaaaatatt agccactctt tttgatcttt acgctgcaaa aattcactta
    21421 cttggatggt ttgctgaatc aaaagattga atgtgagcaa tagactgctc aatatcatca
    21481 gtccaattag acattcaatc agcgtaaatg ctggaattac ttgttttttc aatactgatc
    21541 gcttctttct ccatattgac aaacactttt tcgattactc cattattatc gacaatttgg
    21601 atatcgtatg ggatcatttc ttcccttcgc tctggtcttt tgtgatacat ccgctgcgtc
    21661 ttgatatctt cataaatcat tcgaataaat agcagttcct ccccactttt cttttctgcc
    21721 tgaagcaaaa acagctggcc aaagctcact aagtagacac agacagaagc gatcataagc
    21781 gctgtaaggg actccaacaa aacaaatcct gttttattca attctttttg tatagcgacc
    21841 actccctatc tggaattgat acgtaatcgt ccgctctttt tcttcccaat aaaattgata
    21901 agcttttagt gaactttcat tcccggttct agcagcaaaa gtaatagaag catggcgttt
    21961 caaggtaatc tcatcaggaa tatctaatac ttcccaattt attttatctg gattcggaac
    22021 atgaaaaatg atttgattct cttcactatt ccaataaaag cctgtttgga tctgattcac
    22081 aatagcgatt ttttgcgtag aatagattcg tttttcaaat tggccgaaaa attgatagac
    22141 tgccaattca ttcttccatg cagaaaatgc gagaactgga aaagaaagga tcgttgtaag
    22201 taataaaatc agtaagagtg attcaaacaa cgtatatcct gatgctaaaa ccttaccttc
    22261 aaggccgctg atagatttct gcatgttctc gtttgatata gccttcattc aacaattcct
    22321 ccacagtagg aactcttcca ttattttgaa gcgcatagag ctctttttgt gtgtctacca
    22381 actggatgat cgcttcacga ctttcttggt ctacatgatt cctataacgc gacaaattag
    22441 ggacgaacaa caacactaat acactgatga tcagtaagac gattagcatt tcgatcaatg
    22501 taaaaccgct ataataagat atttttctct ttttcatggc aaaacctcct ctatgcttcc
    22561 gtaaacaggt aataaaacag cggcataaac taataaaatc aaagccgcta cgcccaaaaa
    22621 aatgattggc tgaataaaat gcatccagtg atgaagacgt ttgaaaagct gttttcttgt
    22681 ccattcgctg taaaaaatca gttctttacc taaacttccc tttgcctctc cctgtaaaac
    22741 aattttactg aattcttctg atagaaaacg ataattctgg aactgctcag ccaatgacat
    22801 acctgcctcc aatccttgaa tcaacttaga ggcaagatgc tgaatcagac tttcttgttt
    22861 cgtttccttt aagcagacta tgatttgcct taattcgaaa ccttcatgaa aaagctttcc
    22921 aatctctagt gacaaataag ccgattgata caaggaatat atcggtccaa tcaataattg
    22981 tctgcttatc cattcactct gatcaacagg agacaacttc atcagacgat accttactag
    23041 taaagccaat agagtaaccg ctaggatgaa tagtagaaca taccaatgaa agacttgtat
    23101 cagctgaata ccccaatgct cttgcatgac cattcctgaa ccaataagct gaggcaaaac
    23161 gaacaaccgc atactaaaca agatacctag caaaaaggtg agcaatagca tcggatatgc
    23221 aagcagcttt ttcaaatcat tgcgaaatcc ttcaacaagc cgaaactgtt tggcaatttc
    23281 caacaaagtt gtcggcagat tcccatgacg ttcagctaac tcgatctgta ccagttgctc
    23341 tgctccatat cctatttgcg aaagtacctc actaaacgct tgcccttcct ttagctgatg
    23401 ttggatattt gccaaaataa caggtggaaa cgaacggatc gtgagtaata aataaaggga
    23461 ttcttgcaga ctaaaaccag ataaaagcag ttctcccatg gtttgtgcaa actggttttg
    23521 ctggcttctt gttaatcctt tagaatagtt ctgactgcca aatattttcc tgagcttctt
    23581 cataactttc ctcccatgat ttttttactg attcttcctc cataaattga taactccata
    23641 aaacagaagg atggtgattg gcatcttcca aaagttcttg ataaatgaca ccagctaaac
    23701 attcagatag ctcttctttt ttctttgtca gttcatggat tctgaccaat gtaccttcaa
    23761 gattgcgtgc atgtacggtt gcaaaaaccc ggtgccctgt tagtgctgct cttaccgcag
    23821 catgtgcagt tgattcatct cttatctcgc caatgattag caagtctgga cgatgccgta
    23881 atgcgagtct caaaagtgta tcataagtct gtgctatttt cggattgatc tgtagctgca
    23941 gaaaaaacgg ttcttctatc tctacaggat cttcgatagt aatcacttgc aatttttctt
    24001 ccaaagccaa ccgatacatt agagaagtct tgcctgatcc gactggtccg ctgaaaaggt
    24061 ataatccacg tctgacggtt ttctgtaaaa tagttgcaaa atctcgagaa ctaaagcaac
    24121 gatggggtgt ttcaaaagta ccgtataata gccgaatcac taaactctct cgttgaaggt
    24181 aatctcctac agaagacaac cgcagacgaa cttcctgttt ttctaaagaa taagtaaatg
    24241 cacctaactg agcttttctt ttttctccga tatccatctg tcccaaatac ttaaatcttg
    24301 agatcagttg ctgtccatct gccgaagaga ctttcttcca gaatgttcgt ttttttccat
    24361 agcgaaaata gatgtaaaat acttctgaag ctggcaatat atatacgtct tgggcatgtt
    24421 tggcaaaact ttcacttatc atttgatccg ataatcgttt tatttccatt attctcccct
    24481 ccacaaaaaa catacgtctt tttgatcttt ttgtttcaaa taaaatgaat ttacaaaaaa
    24541 atattggcaa aaaccgaatt tatgttatac ttataaaaat ttatcttggg aggcactcca
    24601 ttggaaacaa cattaaggaa atatatcgaa gtagcaagta aaaaaaagcc tgctgaccta
    24661 gtgatcaagc atgctaagat cgtcaacgtc tttacgaaag aaatcatgga aaaagatgta
    24721 gcaatctgcg aaggaatgat cgtaggaatc ggtgaatacg aaggaaaaag cgtacataat
    24781 gctaatgggc aatatctagt gccaggattc atcgatggcc atgtgcatat cgaaagctca
    24841 cttttatcac caagcgaatt ttcaaaagta tccttgcttc atggagttac tactgtagtg
    24901 acagatcccc atgaaatagg aaacattgct gggagtacag gtcttgagtt tatgatagaa
    24961 gatgcaagac aaacactcat gaatatcttt tttatgctac cttcttgtgt acctgtaacc
    25021 ccttttgaga ccaatggtgc aaatttaaat gcaaaaaaat tagccccttt tttacaaaaa
    25081 cccgaagttt taggcttagc tgaagtcatg aactatcaag cagtagcaaa taatgaaaca
    25141 gacatcctag aaaagatcca attgatgcac cagcataaga aaaaaattga cgggcatgca
    25201 gctggtatcg gcatggaaga gttaaatgtt tatcctgcag caggtattcg gacggatcat
    25261 gaagctacaa ctgcaaaaga agctaaagaa cgtttggatc ttggtatgta tttaatggtt
    25321 cgagaaggga ccgttgcaaa agatctggca tcgctactac cggcgatcac acctgaaaat
    25381 tcaagaagat gcttctttgt caccgatgat aaactgatca atgatttagt aaaagaagga
    25441 tcaatcgatc atatcgtccg tcaagctatt gcacttggaa tcgatccatt acaagccatt
    25501 cagatggcaa cattgaatgc agcagaatgt tttgatttga aaacactagg tgcgattgcc
    25561 cctgggtatc aagctgattt cttcttaaca gacgatctta ccaagctgcc aattcatgaa
    25621 gttttctata agggaacatt agttgttcaa tctgggcaac tccaagacca tttatttgct
    25681 gatcattcaa attcatatac gtgttgtctg ccaaaactaa acgctcaacc attaaatcca
    25741 cattcgttag agctgcttct ttcttcttca caagcccatg tgatcgaaat tatcccgaat
    25801 agtctagtga ctaaagattt ggtagaaaaa gtggaccgac aaggcgaacg attcattcct
    25861 tcaatcgaaa aagaccaatt aaagattgcg gtgatcgagc gtcaccatca aacgggaaac
    25921 attggattag gcatagtgaa aggatttcag ctgaaacaag gagcgatagc gacaacgatt
    25981 gcccatgact cgcataatct agttgtcgtt gggacaaacg acgaggatat gctgtatgct
    26041 gctaatcttt tgattcaaaa aggtggcggg atgaccgttg tcaacaaaca gcaggaaata
    26101 gcttgtgttt ctcttcctat aggcggaatc atgacacagg aacctttttc gatcgtctat
    26161 gaacagttga atgaattaat aactaaagca catcaactag gagcagctaa aacctttgat
    26221 ccttttttga cactttcttt cttaacatta tctgtcatac ccgaattgaa gatcaccgac
    26281 aaaggattat tttcattttc tcgcttccaa ttgattccac cttgtggaaa aaccgatgaa
    26341 ataaatacat tttaaatcaa ataaatccca gaaaatacat tacatctgta atgttcattt
    26401 tctgggattt atttatggat attaatgttt ttatgaaagc agaaagttag tttcacagac
    26461 ttttcttttt ggtcttatct tgaaattgag cttctactcg ataaatacgt tgaccttttg
    26521 aagaaaattt ttcttcatac tccgtcataa tattgccttc aaattgactt tgatgtaaat
    26581 ctaaccatac ctgtttgagg atcatgccat attgagaaaa acttgccaga gaatactcaa
    26641 acaatccttg attatctgtt ttgaaatgga tttctccatt tggacgaaga atctgttcat
    26701 ctacagcaag aaaagtctta tatgtcaatc gccgtttttc atgacgtttt tttggccatg
    26761 gatcggaaaa attcagatag atttgatcta cttcgctgtc agcgaaatac tctgtcaacg
    26821 ctgatccatc cacatgtaga agtttcaggt taggaagcgg ttgttcgatc aacttgtcta
    26881 gtgcaatcga aacaacactg acctgcattt ctactccaat ataattgatt tcaggatggg
    26941 cttttgccat tccagtgatg aactggcctt tccccatacc aatctcaatg tggattggat
    27001 gatcgttttc aaacaattca ttccattttc ccttccacaa tgttggatca ctaattacaa
    27061 aattcgggtg agccgctaac atttccgctg ctcccggacg atttcggaca cgcattattt
    27121 atctccattc ctttcctcta attaaaaagg tctggccaaa tctccgcaga gtttgggcca
    27181 aacctcagcc actgaaaatt cgttttaatt gtaaaatttc ttcgttcatc cgtcgatgat
    27241 cttcacgttt gcaatatctg gtgatttctt ggagcaaatt catggccgca taccaatgaa
    27301 atttttcaag catttcgtca gtcgggcgta tgccataact gatcagccac tgattccagc
    27361 tagacagtgg cacataattt cctaaaatcg ttgcaatatc caccgcaggg tctgcaaaca
    27421 tgattgaatc ccaatcgact agatataaat aatttttaca tactaaccaa ttacgatggt
    27481 ttacatctcc gtgtactact gataagtttt ccttatctat tgacgggatc tgctcacgaa
    27541 gaaaacgatg gacaatttgc agaaattgat ttttctggag ttcttccggt aattcttttt
    27601 catatttttg cagcatcttc tccggcgtca tgacacgacc accgatacgg gtcagcatgg
    27661 ttttaagaga atttgaatga tgtaaatggt acaaaacatc aatcacgtcg tttctttgac
    27721 caatctcatt aggagtcagc aagcgacctt ccagccactc ttgtgctgtc aaagtatcgc
    27781 cattccccgt tcgttttgtc caaacaagtt ttggtgtaat tccttccctt gaaagagcag
    27841 ccaacatcgg agtcgtgttt cgtttaataa agactttgtc ctcatcacgc atgcctatgt
    27901 atgtttttcc tgtgtcgcct ttgatgggac gtaaccgcca ctcttggtct aactgaaact
    27961 ccatcatgca ttctcccgtc ctcttactct ttgttttgca gtacttatta ttctagtcgc
    28021 ctgaacacta cacgtcaagc acatatacgt tctttttcat ttaattgcca ttcaaaaaac
    28081 cagcttttct caaaaaaaat agaacgaaca gcattcttac tgtcaacgct ctatttttca
    28141 ttctcatttt taccctacac agctttgttt tcagtctgac atcttcttca aacgagttgg
    28201 tacatatagt ttaacaaaga gcatagtaac aatcaaataa gccgctagcg gaacccatcg
    28261 ctgctgccca tctaatacga ctgcagcaat caaaccaaaa ataaccgacg cgataattaa
    28321 tacccatgtg agtagatact gaatcgcctg cttcttctga ttcttatgga ttggataaag
    28381 ctgaacgaga accatatagc ggaactggtt cgctaaaggt aacagttgga aaccgatcaa
    28441 atataaaaat aagaaaccga taatcagact aaaataaaag tcgactactc cggcaatcaa
    28501 taacccacct ataattacta atcggaaata aagaccacta aaatcagaac ctcttgccaa
    28561 tcttcttgca aacaagtaaa gataagtgtt ttttgattct cttttaatat gagtcaatat
    28621 aggatccaaa tagcgtcttc ttttgatctt tgccgtaatt tcaggaacat ctgtgaataa
    28681 attgataaat tgatagatct gatgcaacct atgttcttct acatgcacca ttttctccca
    28741 atccaatcct ttttcttgtt gcttccacaa aaaccagtaa tatagacttg cctgtactaa
    28801 tgcacctgct attcctgccc atattagcca ataaaatgat agaaacaaaa agatcagaga
    28861 agaaaaaaac cagcctaatt tccaataacg aataacttgt tgatctagtc gaaacaattc
    28921 tgctctcttg atttgcagat gactgaattt caaactccat aatatgatca gatagacgaa
    28981 aaacatcgaa aaggattgct ccgtactaac caccactaat ggcatggcca atccgcatgc
    29041 caatatcaaa aagatcaacg gaaaaatgca agaatagcga aaagctcgtt ccaaataggt
    29101 ttgcatttct ttttctttag gaagtaaaaa aacaatatct gctgcctctg caaaagtagc
    29161 catcttaccc acgaataaac agagcatcca aaatacagag attatgagcc ctccaattgc
    29221 aaacggtggc tgcagggttt tcaaccaatt agaataatat aatcccacac cgccaatcaa
    29281 gaaaagacaa actaaaacaa aatgatcgtt caatacataa cgcatatatt tcatcatttt
    29341 cttttgatgt cttgctaaac gtttttgaaa aaaactactc atgtgcctgc tcctttgtta
    29401 aagcaatata gatttcgtcc aaagaagaat ccggcagctg aaattctttt ctcaacatct
    29461 caagagaacc gtctgctcgt atctctccgt tatgtaagac cacaaaacga tcacaatatt
    29521 tttctgcagt agctaaaata tgcgtagaca tcaaaatacc ggctccttct tttttcattt
    29581 ctaccatcaa ttcaagcaat gcattgatag ctagtggatc caagcctaaa aatggttcgt
    29641 cgatgatata taaagaaggc tcgatcaaaa aggcgcataa gatcatcact ttctgtttca
    29701 ttccctttga aaaattagct gggaaccagt caagacgatt agataaccga aaagtctcca
    29761 acaacttttc tgctcttttc attgctatat ccataggtat atcgtaggcc atcgcagtga
    29821 cttcgatatg ttctctcaat gtcaattctt catataaaga aggagtctct ggaatatacc
    29881 caatttgttt gcgatattct tccgtatttt tctttaagga tagatgattg atttctatcg
    29941 ttccttttga agcttccagc agaccgataa tgtgcttgat ggtcgtactt ttccctgctc
    30001 cgttcagtcc aatcaaccct actatctctc caggctgtac atcgaaactg atatctttta
    30061 acacagggag atgtccgtac ccccctgtga catgatcgat aattaaactc attctttttc
    30121 ctccgccatt tatttttatt tattatacca cgaagaaaat gacggtattt tgtttttttc
    30181 cttacaatag ctttaactta tggtaaagtt aagctgaagt atttattctt tatagaaagg
    30241 atgttttata tggaaaactg tattttttgc aaaatcatca atcaagaaat cccaagctat
    30301 aaaatatatg aagacgacaa ggtctatgct tttttagaca tctctcaagc aacgaaagga
    30361 cacacattag ttgtgcctaa acgtcatgta gcagatattt ttgaatatga tcccgaactt
    30421 gcaggagagg ttttttcgcg tgtgcctaag attgctcaag cattggaaaa agcttttcca
    30481 gaaatgaaag gtcttaatat cttgaataac aaccgtgaac tagcctatca atctgttttt
    30541 cattcacata tccatttagt ccctcgctac agcaaagagg atgatttttc tattcacttt
    30601 gtcaatcatc aagatagtta tggatcggaa gaactaaaag ctattcaaga aacaatcgta
    30661 aagcaggtgt cctgcgatga ttaaaaactt tttcaaagga ctgttatttg gagctgctgc
    30721 tggcactgtc ggaggtctgt tattggcgcc caggagcggg aatgaaacaa gaaagaaact
    30781 gatcgaagaa ttggaagaag caacaactct gacaaatgat ttaaataaca gcctgaatca
    30841 ttttaaaagc gcattgatcg aaacaaaaga aacagctgaa tctgtgattc ctgctttcca
    30901 agaagcaatc aaaaaagata tcgaagattt taaattccaa gccgaaccac gtatcacaca
    30961 aatcaatgaa caagtagaaa aactaaaagc tgatctgcca gagtatcctg aacaaatata
    31021 ataatgatta ttttctcaac agatttaact atataagtga gaaaagtttg cgtatttcct
    31081 aggaatgtga tgaaattttg aattttgttt gaaactacca cgacaacgtt tttaaaggaa
    31141 atgggatatg gtatagttaa tatataaaaa taataactaa ttacaggagt gcaaatttta
    31201 atgaaaaaaa aatcaattat tttggcagct actagtgcgt tggctgtttt aactctagca
    31261 gcttgttcag gcgatactaa taaagatatc gcaacgatga aaggcggaac gatcactgta
    31321 tctgatttct atgatgaagc aaaacttgaa tcatcgaacc aatccttagt acaacggatg
    31381 atcatttata aagtattcaa taacaaatat ggcgataaag taaccgataa gcaagtggat
    31441 gcagaatacg acaaacaagc gaaatcatta ggtgatactt ttgaatcaca attagaagct
    31501 gctggatata cgaaagatac ttataaagaa tatattcgta acaatttagc ttttgaagct
    31561 ggcttgaaag ctcacgtaga tattacagat gacgatttga aaactgcttg gaaatctttc
    31621 catccagaag tagaagcaca aatcatcaaa ttatcaagtg aagatgaagc aaaagatgtg
    31681 aaaaaatcag cggatgatgg agatgatttc tctaaattag ccaaagacaa atcaactgat
    31741 acagagacaa aagaagatgg cggtaaagtt aaattcgact caacgacaac aactattcct
    31801 gcagaagtaa aagaagcagc tttcaaattg aaagacggtg agatctccga tgttattact
    31861 acaacaaatc ctacttctta tgcgacagaa tactacgttg tcaaaatggt gaaaaaccaa
    31921 aataaaggca atgacatgga taaatacaaa gatcaactaa aagatatcgc tactgaaact
    31981 aaactaagtg acaatgcatt cacaactaaa gtaatcggtg aagaattaaa agatgcaaat
    32041 gtaaaaatca aagatgatgc ctttgaaaat gtgttgagtg cattcacaac aacttcatca
    32101 agtacgaaag actcaagtga aactacagct tcaacaaaga gttctgacac aaaatcaact
    32161 gattcaacaa aagaatcttc aactgaagaa acaactgatt catcaaaata aaagaaaaca
    32221 aaaaaagcga agacttatgt ctcgcttttt ttgttttgaa tgaaaaaaat aaatttttgc
    32281 tataaatctt ttggtacata gaaacttcgg ttgtccatac cgaaaatccg atcggtgtat
    32341 tttcctggtt ctgtttgacg aatcgaagtc aacatcatct gcatcgaagc atcgatattg
    32401 tcgatttgat gtaatatttc tgcttccatt attcttggac gtacaggtga gccatattct
    32461 aataagccat ggtgtgctaa aaccatatga cgtaatacga gaacatcttc gtcagcttcg
    32521 ctgattttca acgcaagaca ggctttagtt atttcttcgt caatcaatac cagatgaccg
    32581 atcaaatttc ctgcaagcgt atattctgtc gacataggac cggaaagttc aatcaccttt
    32641 cctaaatcat gcaaaatgat accagcatac aacaatgctc gattcagctc aggatattca
    32701 tcggcgattg atttccctaa gtgaagcatt gtcaaagtat gataagctaa tccactggca
    32761 aatgcatgat ggtttctttt tgctgccgga aactcaaaaa attgtttttg atatttatta
    32821 agtagatagc gaacaatacg attccaatgc gcattggtga tctcaaacaa caattgattg
    32881 atttcttctt ccatttcttc tttcttcaaa ggggctcttt ccatgtataa actaggctct
    32941 cctggctcat ctacccttgt taagcgcata tgaagaatct ttacttgagg gtttccttga
    33001 taaacctctc ttttaccgtt tagaaaaacg actgtgccgg ctgtaaattt attgatttcc
    33061 tcctccgaag catcccagta tttaccatcg atagtacctg aagtatcttg gaaagtaaaa
    33121 gcaataaatt tttttccgtt tttagcgatt cttacatctg cacttttgat caggacaaat
    33181 tgttcaaact gctcatcgat tactaaatca cgaatttttt tcattatttg ctccttctaa
    33241 ttgttttacg atttggttca tctcatggta atagcttacc atttctttat cagaagaaaa
    33301 acaaattatt tgatactttt ctccaaattt agctaaaagt tcagctaatt gatactttcg
    33361 ctgatgatca taatgaagcc agccatcatc aatgatgatc gggcatatcg aacggttagc
    33421 ttctaatgag aggtaagcaa atctcatcgc cataatcagc tgatctttcg tacctgtaga
    33481 taactcatag atgggaaatt ctgtatatcc gtctgttaaa gtcagtgtat catttttcag
    33541 taatatcttc tgataatttc cttttgttaa aatacctaaa taatcacctg ctgctcttaa
    33601 caactggggt aattgtcttt cactcatctc tgttgaaaga tcacctaaga atgtcccggc
    33661 gatttcaagt gctccccatt cctccagtaa ttcctgtaat tttgcttttt gccggctagc
    33721 ttgttgataa agttcgtcca atgtgccgtc tctttgcaac tcttctactt gaagttggag
    33781 tcgttgccga ttttcacttg cttctttttc ttcttgctgg agttgggatt gcttttcttt
    33841 cagttggcgc agtcttacag ataatttctc ttttgtcagc tcttcaggaa atagtggagt
    33901 aagcatttcc gataattcat tgtatctttt catctgctgt tcggcttgtt tggctttttg
    33961 taaaaagaca ggtacttccg aaggataaga taagcctgct tgagtcaatt gttcgctgta
    34021 ttcttgtatc catacttttt gtttcttttt caattgattg atttcttgct tcaataaagt
    34081 attttgctga tagcttctgg caaatttcat ctgttccatc tcttgtataa aacgggaaag
    34141 caataccatt ttttctgaaa ttgtcttatc ttgtatcggt aaccattctt taacaaactc
    34201 aaatttttgg tcgatttgtc gtagtttttc ttgtatttcc tgttctcttt tatgtttccg
    34261 ctcaaatacg ccatataatt tctgataatc tatgacttcc tgtccatatt ctttcatgag
    34321 tttgagcgta tccatctttc ctaagtgccg atgatccgca aaacgatata cttcttgctg
    34381 aagaatctgt ttttcttctc cagttctctt ttgtttttct tctgcttcag ctaattgagc
    34441 taaggctaga tcaagttgtc ctagtttttc ttgccacatt ccttttatct cagagaaatc
    34501 atttttagtt ttaatttttg gttgcctttt gtagaagaaa tagccaatca tgcctgcacc
    34561 aattagtaaa aggatcactt tcaagggcat tggtacaaat atacctgcaa tgattattgc
    34621 tccgccagcc atcagcatat acggtttaga tgaagagact ggtgctatct ctgactgatt
    34681 ttgtaataat gctggatagg tctcttccaa gtcattcact tctgcttcaa tcatctcttg
    34741 ctgttcttta gctatttgga gacgcacaga aatctggctt tctagatcag caatccttct
    34801 ttcttcagcc agaagatgct tccattcttc ttcatataaa aattcttccg gtaacggatt
    34861 tttctgccaa tgccaccttt gttccaaaat cttcaattct gaatttgttt ctctagtctc
    34921 aatttgtaat ctttgccatt cttcgtttaa ttgttccgct gtaaattgaa gttgctggag
    34981 atcttttagt tcctgctcct gctctaaata aaaataatat ctggctgact gctgatccat
    35041 cccgctatgc ttttccagtg ttgcttctaa ttcttccaaa cgctcatttg tttgctgata
    35101 ccgtttggag aaagactcca gatctaaaag aagttgctca tcaggaagac ctgtagtgct
    35161 gttttttttg agagattgcc attcttcata taaagaaaaa ttaagttctt gctgtctggc
    35221 taatgaaaat tgtttgttcg cctcttttaa ttgtgccaat aactgctgct gttgcgactc
    35281 gtattcctgt tcttgttgga tcaacttgcc aaaataagct tcctgttctt gtttttgatg
    35341 aattttttct tgcagctctt tccattcatt taatttttga ttgattagta atttttggcc
    35401 ttttttcttg aataattgtt gtgctttctg ataatattct tgccgctttt ggaatacttg
    35461 agaactgccc gtgatgccta aagaaatcag cgcatcatgt agctcttttt ctctcaaatg
    35521 atctagttcc atcaactgct cttgctggaa agtaaatacc tgctggaata actctttcgt
    35581 caaaggtgcg atcagttttt ctagtaactc atcacttccc acttgatccc ccagccacac
    35641 cttcgatttt ccgcgatttt gctgcttgta gcgctcgaca gcaaattcac catatacagg
    35701 atgtttaagc catattttgc cgccataggc tgagccgtct tttggtgtat aatccttttt
    35761 tttacggcct tttgaaggga aaccaaataa tattgcttga ataaattgat aaatcgttga
    35821 ttttcctgct tcgtttcccc catagatcag ctgattgcct gaataaaagt caaaactttt
    35881 ttgacgccat tttccaaaag cagcaatctc gatccttaaa atcttcattc ctgatcctcc
    35941 tcaaataaaa aatcttgatt aagcagctgc ttggcttttt caagtgtttc ttcttgatat
    36001 tccggcagtt cgctcaatat tctagccgct tcttgatgac tatatacttc atttaaaatt
    36061 tgttggaaat cttgaggtgt ctgatagtat tgaaataact gttccatcaa tttagcagaa
    36121 gcagcaagtg gggtcttcgt ataaaaagta ttctctatga gagaaatacg ccaaataaag
    36181 aaatcattta tcctatctga gaattcctct aacaaatagt ctaatagctc tcctgattgt
    36241 atccgttcca aaacatctgt attgagatgg tcataatctt ttaattttat ctcaattaaa
    36301 ctaaatccat cctctacttt tgaaagctgc tgacgaatgt gaacaatgac ctcttttgtc
    36361 gttcttgctt tcctcaatga tatttccttt gtcttccaat agacctctgc tactttaatc
    36421 ggatgtattt ggcaacttcc tttacttaat tcaactagta gaatagatgt agaagcttgt
    36481 tcctttttag tatgaccttg aggcgcacct ggataaacaa ttgtttgctt ttcatttaag
    36541 accgttggca catggatatg ccctaaagcc caataatcgt atcccttttc ttgcatcttt
    36601 gagggttgga aaggagcata gtttcctttt tgagagacgc caggttcccc gtggtacaac
    36661 ccaatatggt aatctgtgag ttctctagaa ggaaattcca tcactttgtc tttttgaatc
    36721 cacggttgac gataactaaa tccactgaga gagacagttt ctccactttt tatcgttatt
    36781 ttttttgtct ctaccgtttc ggatgtaaat aaatggacat ttttaggaaa ggagaaccaa
    36841 taccgctctt tttgatagaa atcatgattt ccgaatatta taaatacagg tatatggttc
    36901 ttttctaacc gtttcatctg ttctgaaaaa tgtttctgta tttttaatga aggacgattt
    36961 tgatgaaaat tatctcctgc aagtaaaaca aaatcaacgt tatttattat tgcctgttca
    37021 atgatatttg ataaaacggt cagattcgtc ttcaatagtt tttcctgtac agttttatca
    37081 agagttgtca gtccttcaaa tgaccgatcc atatgtaaat ctgccgcatg gataaatttc
    37141 accattaaaa atttcctcct ttttttcttt tatcatacca ttttcaatgt tttttttcat
    37201 caataaccga aaatttgttc gtattatgca aagaaattac gataaatggc cgacttcaaa
    37261 aaataagttt attcgaaaaa aagattgtga catgattttt gtcacaatcc taaatatcta
    37321 tgttatattc gcttcgtttg acactgaaga agtactattt gtactggaat ttatgctcca
    37381 tttttccgta tttagaattc tatgttctat actaccttaa ttagtttata tggtaagaat
    37441 tctttcaaat gcttgagata ctccgtcttt atcatttgaa acagtgacat aatcagcttt
    37501 tttcatgatt tcttgatgac tatttcccat tgcaaaacta atatctgcac tttcaaacat
    37561 agataagtca tttaaactat ctccgatcgc gattctcaaa ggtttcttta aattgtagat
    37621 tgattccaaa cgatcaaaca ccacaccttt agatacattt tgaggtacga tttcaatatt
    37681 tacaggtgcc gattcagtaa cagacacatt tagcatcttt ttcaaatggt taaatatatt
    37741 agctttcaat tgacgattag gaataaaaat aaaaaatttg taaatgctga tagcatcctt
    37801 ttctataatt tcctcaatat tattataagg gattgaacaa atagattttt catactcaag
    37861 ctcatagttt agctctttga tagaaaattc actccctacc tcgtgaaaaa tttttttcat
    37921 tttattttta taattctgag attcataact tccgagattt gtatatattt tggttggaaa
    37981 tttcgatagg acagaatcaa ttttttttac atcattttta tcgaaggaag actcaaatat
    38041 ctttttttct ccatcaaatc caacagaacc atttaaccct actgctggta tccttaaacc
    38101 agattttttg agaatattat taatgtcttc tacttctctt ccagaacaaa tgaatggaaa
    38161 acagtcttcc ggtaaatgtt tcattgttct tgcatttttt tgactaatgc tctggtcaga
    38221 attcaaaaga gtaccgtcta aatctatacc aattagtttc atttcatcca tgtctcctat
    38281 acgttgatta taaattcact gcttattgta agtctaaatt tctcacaaaa catatactaa
    38341 ttatacctaa tttaagtaag tacaaacatc attttaagaa taggaaagga catttatata
    38401 ctattctctg tatttttgat ttataaataa atatgaattg aatccaaatc agctatatta
    38461 ctattttata ataaccaact ataaaaaacg aagttaggtg aaaaactaac ttcgatattc
    38521 ataaaagact ctattagttc aacttagcaa aagatttaaa
//
LOCUS       DAZKMK010000014        36779 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000014 DAZKMK010000000
VERSION     DAZKMK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.014"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..3436
                     /locus_tag="ACG8V1_000776"
     CDS             158..3436
                     /locus_tag="ACG8V1_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0328892.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3977..5884)
                     /locus_tag="ACG8V1_000777"
     CDS             complement(3977..5884)
                     /locus_tag="ACG8V1_000777"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0328893.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6014..6889
                     /locus_tag="ACG8V1_000778"
     CDS             6014..6889
                     /locus_tag="ACG8V1_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0328894.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6969..7943
                     /locus_tag="ACG8V1_000779"
     CDS             6969..7943
                     /locus_tag="ACG8V1_000779"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0328895.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8123..9268
                     /locus_tag="ACG8V1_000780"
     CDS             8123..9268
                     /locus_tag="ACG8V1_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0328896.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9405..9749
                     /locus_tag="ACG8V1_000781"
     CDS             9405..9749
                     /locus_tag="ACG8V1_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0328897.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10020..11306
                     /gene="efmA"
                     /locus_tag="ACG8V1_000782"
     CDS             10020..11306
                     /gene="efmA"
                     /locus_tag="ACG8V1_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0328898.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11444..12043)
                     /locus_tag="ACG8V1_000783"
     CDS             complement(11444..12043)
                     /locus_tag="ACG8V1_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0328899.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12447..13568
                     /gene="mnmA"
                     /locus_tag="ACG8V1_000784"
     CDS             12447..13568
                     /gene="mnmA"
                     /locus_tag="ACG8V1_000784"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0328900.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13814..15235
                     /gene="arcD"
                     /locus_tag="ACG8V1_000785"
     CDS             13814..15235
                     /gene="arcD"
                     /locus_tag="ACG8V1_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0328901.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15249..16367
                     /locus_tag="ACG8V1_000786"
     CDS             15249..16367
                     /locus_tag="ACG8V1_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0328902.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16427..17719)
                     /locus_tag="ACG8V1_000787"
     CDS             complement(16427..17719)
                     /locus_tag="ACG8V1_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0328903.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17869..18429
                     /locus_tag="ACG8V1_000788"
     CDS             17869..18429
                     /locus_tag="ACG8V1_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0328904.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18558..18935
                     /locus_tag="ACG8V1_000789"
     CDS             18558..18935
                     /locus_tag="ACG8V1_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0328905.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18868..20940
                     /locus_tag="ACG8V1_000790"
     CDS             18868..20940
                     /locus_tag="ACG8V1_000790"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0328906.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21151..22098)
                     /locus_tag="ACG8V1_000791"
     CDS             complement(21151..22098)
                     /locus_tag="ACG8V1_000791"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0328907.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22344..22808
                     /locus_tag="ACG8V1_000792"
     CDS             22344..22808
                     /locus_tag="ACG8V1_000792"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0328908.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22870..23913)
                     /locus_tag="ACG8V1_000793"
     CDS             complement(22870..23913)
                     /locus_tag="ACG8V1_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0328909.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24290..25255)
                     /locus_tag="ACG8V1_000794"
     CDS             complement(24290..25255)
                     /locus_tag="ACG8V1_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0328910.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25489..26334
                     /locus_tag="ACG8V1_000795"
     CDS             25489..26334
                     /locus_tag="ACG8V1_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0328911.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26525..27712
                     /locus_tag="ACG8V1_000796"
     CDS             26525..27712
                     /locus_tag="ACG8V1_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0328912.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27718..28074
                     /locus_tag="ACG8V1_000797"
     CDS             27718..28074
                     /locus_tag="ACG8V1_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0328913.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28076..28414
                     /locus_tag="ACG8V1_000798"
     CDS             28076..28414
                     /locus_tag="ACG8V1_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0328914.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28490..28746
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28809..29843
                     /locus_tag="ACG8V1_000799"
     CDS             28809..29843
                     /locus_tag="ACG8V1_000799"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0328915.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29836..30522
                     /locus_tag="ACG8V1_000800"
     CDS             29836..30522
                     /locus_tag="ACG8V1_000800"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0328916.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30546..31394
                     /locus_tag="ACG8V1_000801"
     CDS             30546..31394
                     /locus_tag="ACG8V1_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0328917.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31591..32364
                     /gene="sufC"
                     /locus_tag="ACG8V1_000802"
     CDS             31591..32364
                     /gene="sufC"
                     /locus_tag="ACG8V1_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0328918.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32377..33663
                     /gene="sufD"
                     /locus_tag="ACG8V1_000803"
     CDS             32377..33663
                     /gene="sufD"
                     /locus_tag="ACG8V1_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0328919.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33660..34895
                     /locus_tag="ACG8V1_000804"
     CDS             33660..34895
                     /locus_tag="ACG8V1_000804"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0328920.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34882..35352
                     /gene="sufU"
                     /locus_tag="ACG8V1_000805"
     CDS             34882..35352
                     /gene="sufU"
                     /locus_tag="ACG8V1_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0328921.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35357..36748
                     /gene="sufB"
                     /locus_tag="ACG8V1_000806"
     CDS             35357..36748
                     /gene="sufB"
                     /locus_tag="ACG8V1_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0328922.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        12021 a         6791 c         7870 g        10097 t
ORIGIN      
        1 ttttccgtta ataaatctct ttattatcaa caacttaagt tatatttttt ctttttataa
       61 aatacactta ttataatttt gttttttatt gacagtaaaa ataaattagg taatacttta
      121 agtaaacgct ttctaatttg ttacgggagg aatacacttg ttaaaaaaac gtataaaata
      181 cagtgcgcta atgatgaatc tccttatgct cagtaccccg gttttaattt ctgtcgttga
      241 agttgctgct aacgaacaac agtctgtgaa cgaagaaaat gcacacgtga acattgaagg
      301 gatgtcaaca atggaggaaa taccaagaac tagtgaagat atggatacgg aagaaggaaa
      361 tgatcctatg atgaaagaat cagaaaatga aaagacaaaa aagcaatctg aagaagcaaa
      421 tgaagagtgg aaaggggttg ttttcggaga aagcacaagt gctagtaggt atgccattga
      481 accattagaa gacggcgtgc ggctctcttc cacgaataac ggcggaaaat tccagtcaag
      541 cggatcagat ggaatgacct attattatca agcaatcccc aaagacatca actttaggat
      601 gcgggcgacg atcgaaatag aatcttggac ctatacgaat gcccaagaag gatttgcttt
      661 aatggtaaga gacgcagtac caaaaaatca tttgtttggg caggcatttt attcaaattc
      721 atttgccata ctaggcagtc ggatcgaata tgtctgggac tcggatagac aagaagtggt
      781 caacacgagc ggcagcaaat atacgatgcg acttggattg gggacacggg caatcacagg
      841 aatatcatct gaagatccgc aagctgcgcc agctcctggg agtgtctcag tggaaacaac
      901 acctttagag acttcagcag ggttcttgga aaaagaaaca ggttcttata atatattcgg
      961 aaacggtcat gggaatctgt ttccggccac gaactcgatt actaaactgg acgtagagat
     1021 gaaaaaaacg aatactggtt tagaggcata ttattacgat cctgaaacag gagaagaaat
     1081 ggcttctgac atcatgtatg actgggaaaa attatacgct tcagatgaat cggttattta
     1141 tgcaggattt gctgcagcaa ggaatatgac gatcaaggta gaaggaatcg aagttgctta
     1201 ctcagatcct gcgacagatc ctcctgggca agaaagacct actcgcttga ttgatccgat
     1261 ttacactgtg acctctagta atcattcagg aaaccaagat catactatgt cctttagagc
     1321 aaatgctgac ggattattga caattaaaaa taaagcaaca ggagaggtgc ttaagaacgc
     1381 aaaaaacttg gctatcactg cgaatcggga atttgattat cagacaaaac tgatggaagg
     1441 aacgaacgaa tttactttca gttttacgcc agataaaaac tatccgccag aagaatacgt
     1501 agagatgact agttatgaaa caaaagagat tcttcataca gtggactacc gtaaacctaa
     1561 atattcgaca gtttatgtaa caccagaagg gtcagatgca ggaaacggaa gcagacaaaa
     1621 tccattatct ctgacacaag cattgagata cgcatatgct gggcagacaa tcgtcatggc
     1681 gcctggaact tattccttcg aaagaggatt gacgattcca aaaggcgtta acgggacaag
     1741 agaaaacccg attcagttga ttgcagagaa aaatccagaa aacaagcgtc ctgtacttga
     1801 ttttaaagga actggaaatg ggatgacact gttcagtcac tactggggtt tacgagggtt
     1861 tgacattaca aatagtgcag ctatgcaaaa aggattacag atttctggaa atcataattt
     1921 gatcgaagag attcatgctt atcggaatgg aaatacagga atccaaatca gcggtagctc
     1981 aaatgatcct ttcgaagcat ggcccgcgca taacttagtc aagaactgta catcttttga
     2041 aaatgccgat ccaggatttg aagatgccga tggatttgcg gccaaactga caataggaga
     2101 aggaaatgta tttgacggtt gtatcgcgta tcataatgca gatgacggtt gggacctttt
     2161 tgctaaaaat gaaacaggtt cgatcggaaa agtaatcatc aaaaactcag ttgcttatcg
     2221 aaacggctgg gtaccaggaa ttgagggaga aggtaacggt aatggattca aaatgggcgg
     2281 ttcttcttta acgggccctc atgaattgat caatagtttg tctttcgaaa atcttgcgaa
     2341 aggaatcgat tccaatagcg gaacagatat tatcgtcaat agcagtacaa gttttaataa
     2401 cggttcatat aatgtggctc tttatacctc ttcagcagga aacacagact accatgcttc
     2461 tggtatctta tctttccgaa cagagaatct tgagatgaga gagcagatcc gcccattgaa
     2521 tcaaaacgaa gatcaaatct accactcgtt gaactactat tggaatgaac gagagaaaca
     2581 atcagaaaat actcttggac ataccatatc aaaagattgg atcgagtctc taaatacatg
     2641 ttcgaaagaa ggtcagcagc ctgttacgcg ctatgaaaat ggaagtatcc atgttcatgg
     2701 actgatgcag attaaaccgg ggaatcgtca aacagaaaag gaaagagtgg atggtcttcc
     2761 attatctgta ggtgctattt ttgacggaga aacctcaccg tcttcggaat atccagagta
     2821 tgcaatcgta caagaaccaa atcagacaaa accaaatgaa caaaataatc cgattattat
     2881 acaagaagca aatggtgtgg cggagaatgc agaaggaaat atcattgaac tgaagccttt
     2941 tacagttttt gtgtcaactt tcacaccaaa tcaaggaacg cctgtctttg aagatatgcg
     3001 ttatttccca gaaaagatcc aaatcgttga aacacagtca agtgctcaat tttctaatca
     3061 taccgctatt ttttcagcac cttcaaaagg agaatatacg ctagaagtca cttatcgttc
     3121 ggaaatattt gatggggaaa aatggtctgc tcatacagaa tctgttaccc agcaaaaagc
     3181 gatcactgtg cgagaaactg ccgatgtgcc cccgggaaat gacaaaccag aaagagaaaa
     3241 tccaggaaat gaatcaccaa atggaaaacc aaatggaaaa ccttctcatc cggataggac
     3301 agcaaaagaa agtgaaaaaa atggcactca aggactccca aaaacgggag aatttaataa
     3361 cccgttgcta gcattagctg gaggaatttt gttaatcggt gtagttgttt atgtcatgaa
     3421 acagcgaaaa aaataagtgg aatttctcct tttatgatgt aaaacaattg actgaggaaa
     3481 gtaaaaatag aaggaaatga gaccaataga tgtctcactt ccttctatct ttttatagat
     3541 gaggttctcc ttgctgcttt tgattggctg ttcttattac ttgatgcagg acagcttttt
     3601 cacaatctct ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg
     3661 gcaaaatatg aggaactatt tttccgtttt tttctctaat actcaggtca ggacgctgat
     3721 ttcacaacct ctcgataaac ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta
     3781 tgcaaaaatg tggaaagcca ttttcacaaa atttttctta ctacttgaaa ctaagcagct
     3841 ttttcacaac ctctaataca tggtattcca gaagtaactt cttcgaaaaa taagccgaaa
     3901 tgtcacaaaa atttgagaag cgattttccc aaattccttc ttatttcttg aagctgacca
     3961 cttctggcac aacctcttac cagattcgca ctcttttttc tggcgctaga tacatagcgt
     4021 cttgttccgt gataccaaat gtttcataga attcgtcaag gttttttggt tgcacgtttg
     4081 ctcggagttt agcaggggca tggacatcga tttgcagtaa aagttgttgg tattcttttt
     4141 ttgcttttgt acgccaaatc gttgcccaat tgacaaagaa ttcctcgata gaaagatctg
     4201 attccgtttt tgctgcttcc aatgcacagc ttagaccgcc tgcatcagca atattttccg
     4261 aaacggttaa ttttccgttt acttttccgt cagcaaaagg cagaccatcg aattcttcga
     4321 tcattgcttg tgctttgttt tgaaattgtg taagatcttc ttctgtccac cagttgttca
     4381 agttgccata ttcgtcaaat aaagcgccgt tattatcaaa tgcatgggaa atctcatgag
     4441 cgataacagc tccgattcca ccataattag cagagcttga ttgttctaga ctgtaaaatg
     4501 gtgcttgaag gatcgctgcc ggaaagacga tcacattccg aaatggatga taataagcat
     4561 tgaccgtgtc agcactcatt tcccattctg aacgatccac tggtttgttc catttgctga
     4621 aacgatcttt taatgtgagt ttgctgaaaa caagagtatt tgaaagcaaa gtaccaccct
     4681 gatctgcagg tactgttttg aactggtcga ataatgctgg gatcttatcg gggtagccta
     4741 cttggatacc taagttattt aattttatga cggctttttc ttttgttgca tccccaagcc
     4801 agtcattttc ttctagacgt tttttataaa cggcgatcat ttttttcacc atttgttcta
     4861 cgtctttttt agcagcttcg ccaaaatatt tttttccgta gtaatcgcca actacttgat
     4921 cgaattgtcc agaggcaagg taatacgctg cttttttctg aggcattgct tcgtctgttc
     4981 cagacaatac gcgagaatag attccgctga cttggcggaa ttcttctgat agataaccac
     5041 taagagaacg tactgtttta atgatcatcc agcttttgaa taacggaaaa tgagcttctg
     5101 tcaggatgtc attcaaatga tcgaagtaca ctggatcagt tacgatcact ttatctggag
     5161 ttgtcccaat caaacccgtg acaagttttt tcaagtccag tttatctgtg tatttaataa
     5221 atgtatcaaa agattgcgga ttatacattt tgctgtaatc tgcgttttct tctgcacttt
     5281 ttacatgagg agcaatcaat ttatcaaatt gcactgcttg ttccgtgatt gcttctgcct
     5341 gacttttttc tttgccggca agctctaata actgaacagc catatcaaag aaaacgccta
     5401 ttagttgtgc accgttggga tggtctgatg cataatacgt cttatctgga aggaataaag
     5461 aaggcggata agcaaagaag gcgttcgttt gcgcattctt catatctgca tctacatcca
     5521 gattaaatgg caatggtaat gtatccaaca gccattctgg aaattgctta ttcaattcgt
     5581 cgaaagactg caatgattca actttttcga ttataggttg aagcggttga gcatgcaact
     5641 catttcttcg gtcataatcg tttgccagtt gataatatgc aaggaaatgt ttcatttttt
     5701 ctgaagacac tttttctgga tgggcaagca gatcgtctgt ttctcccatc aaaagttggt
     5761 cgatcccgtc aactaaatct tggaaaccgc ctgtagctgg tttatctgca gggatttttg
     5821 ctgttttttg ccattcttca ttgactgctt catagaaatc ttgtttcaat cgttctttgt
     5881 tcattcatcg atcgctcctt ttttgtttcg tgtgcccatt atcttacaaa atgaaggaat
     5941 taacaaatag aacataaaaa attgaaaatt cgaacaatca aactgatttg tttttttcaa
     6001 tccttcactt ataatgaaaa aaaggaggcg atcccaactg aatcaagtag tggataaacc
     6061 tttatatttc aaagtagata aaaaaattaa aaaattagcc agcactcagc agctgcagtc
     6121 aagaaaaagt aaattgcttt ttttagctct ggtttttgaa gatcaaagtt atgtgattat
     6181 cgatcaatca ggacatccgg tagaatattc tccagcaaaa tatacttatc aagagggtct
     6241 ttcttgtaaa aaatggaagc tgatcaatga agagccaatc gagttatcta gatggatcaa
     6301 cagaaaagaa gatattcccg ttttgatcga agaaaaaagg tcaggaaagg aactcgcaaa
     6361 ttgctgggtg ggattgccag aagaacgttt tttgaggtat aagaaatggg ctaccccctc
     6421 aggctatctt tgtggtactt acgcggcagc agttctcctt gcctattatc aagactatcg
     6481 taaggggtgg atgcttccaa atgagattcg caagaaaaat acggctgatt cgttggtttt
     6541 gacgaaggca ttgcgtagcc agatccaacc actaggactt cctacgattc cttttcaggt
     6601 tagtacaggg atttctagtt ttttaaagaa gaatggcaat catgagcgtg cacgagcaac
     6661 tcttctaggt tcatggcagc gagctacaaa acgtatacgt gaaggcaaac ctgtgatgat
     6721 tggtattttg aaagtacttg gcagtacata tggcaatcat tgggtgacag cgtacgctta
     6781 ttttgaaact gaaacaggag aacgttatta caaagtacac gacaactggg gcgattatca
     6841 taaagtcatt cctgccagtt ggagtaacgg aacagtttca cttccttaag aatttgctga
     6901 aattaacaga aaatcttgga aaaatatgtt aaactatcga agaatagaga agttttgggg
     6961 aggactacat gagtaataag tatcaggatg acacgttgaa gatttttagt ttgaatggca
     7021 accgtccatt agctgaaaaa atcgcgaaag tgttcggaac agaattaggt aaaagcgtcg
     7081 tgaagcaatt tagtgatggc gagatttcca tcaatatcga agaaagtatt cgaggggatc
     7141 atgtgtatat cgttcaatca acgaatgcac ctgtcaatga ttactatatg gaactactga
     7201 tcatgatcga tgcaatgaag cgagcaagtg cgaaaacgat caatgtcgta ttgccttact
     7261 atgggtatgc taggcaagac cgcacggcga aacctcatga accgatcact gcgaaattga
     7321 tcgctaactt gatcgaagaa gcaggggcaa ctcgagtact gactttagat ctgcacacgg
     7381 ttcaagtaca aggattcttt gatattcctg tagataattt atttacgatg ccgttgtttg
     7441 ctcattatta ccgtcaatta ggtctgacgg gagatgacat cgtagttgtt tcgccaaaaa
     7501 acagcggtgt tcagcgtgca cgtagtcttt cagaatattt gaacagcaca ttagccatcg
     7561 tagatcatgc agatgatgct tcaactgagg gtggttatgt gatcggtgat gttcaaggaa
     7621 agacatgtat catggtagat gatatcttga acacaggagc aacgcttgca cgagcagcaa
     7681 atgtattaaa agaaaacggc gcaaaagaag tatatgcctg tgcgtcacat ggattattgt
     7741 ccgatcatgc gaaaagtatt ttagacaatg caccaatcaa agatatttgt atcactgatt
     7801 ctgttttcac agaagaaaat cgtcatccag aaaacttaac aatcgttaca tgttcagaat
     7861 taatgggtga agcggtcaaa cgcatccatg aaaatacacc gatgagtccg ttattccgtc
     7921 tggaagaaaa agaattcgaa taaacaaaaa aaagagggaa agaagttgtg acagaagtga
     7981 gcagcttcaa ggagctgcga caagactttt gtcacagctc cttttttcta tgcccatgaa
     8041 acgtcctcta gacaaaaatg agctcttctt tggtttgttc gctattttat gctagaatgt
     8101 gcctagcaat aatttggaag ggatgaatga agtgaaaact atttatctgg atcatgcggc
     8161 aactaccccc atgcatccac aagtaattga agcgatgacg gcaatcatgc aagacacgtt
     8221 tggaaatcct tcaagcatac atggatttgg tcgccacgcg catgaaaaat tggaaacagc
     8281 aagacaaatc attgcagaca gtctgcaagc aaaacctcac gagatcatct ttaacagtgg
     8341 gggaacagaa ggggataata cagctatctt agaaacagcg ttctcgcgtc aaaaagaagg
     8401 cagacatcta atcactacag ctatcgaaca tcctgctgtt cttaatacca tgaaatattt
     8461 agagacaaag gggtttgacg ttacatatct gcctgtagat gaaaaaggga atctgtcgat
     8521 cgaagaggtc aaaaaagcgt tacgagaaga tacgatcttg gtctctgtga tgtacgggaa
     8581 caatgaaatc ggaaatttga tgccaatcaa agaaatcggt gaacttttag cggatcatcc
     8641 agcttatttc catacagatg cggtacaagc gtacggaaac caaacgatcc atccagaaga
     8701 attaggaatc gatttattaa gtatctctgc tcataaaatc aatgggccaa aaggcgtcgg
     8761 tttcttatat aaacgagacg gagtaaatat ccctgcattg cttttaggcg gggagcagga
     8821 agaaaagcgg cgtgcaggta cagaaaattt agcagggatc tggggtatgg caaaagcagt
     8881 tgaactgctg tcacctgaag aacgtgaagc acgtaacaac aaatataaag gattccagca
     8941 attgattctt gatacattag aagcaaacga aatcccttat caagtaaatg gtgatttaac
     9001 gaataaactc cctcatgtgt tgaatcttcg cttaattggc gaagcaaatg atttgctact
     9061 gatgaagctt gatcttaatg gggtcgctat ttctactggt tcagcttgta cagcaggaaa
     9121 tatcgagcct tcacacgtat tagaagcaat gtatggagaa gatacaccga tactaaaaga
     9181 atccatccgt atcagctttg gtttgggaaa taccgaagac gacatccagt atttccttga
     9241 tcaattagtt cgtgctgtca aaaaataact agtgaagtca ttcttatatg aacagatgtc
     9301 tgagagaacg ccttcatttt ttagggaact taactgaaaa acctagtaaa aatcaggata
     9361 aacttttata ataaagagaa actaatttta tcgaggtgaa aatcatggca tttgaaacaa
     9421 cagcaacggt actgggcgca ccaattagct atcgtttgca tcctgatgca aaaagatata
     9481 cattgcgcga taatggattt acagaaacaa acggaggcaa ttatcaattg atccgaccat
     9541 tagatgccac gccgcaaagc aaagaaggat tcaaattaaa aatcactgta tcaaaagata
     9601 ttcaaacatt gaaaatgtcg atcactacag caaatggatt gaaagcagtc aatattttca
     9661 aagatccaaa acagaaaatg agccaagata aattttattt tctgatggat gggatgatta
     9721 gccgtgggtt attcgaaaaa gtcgaatgat ctgtggatat aaaaagacaa ctagttttga
     9781 aacggcctat tgttagatga ctaactttgt aaggacgacg caaattagtt gtctttttta
     9841 tttttaaatt taacacatgt tgttattttt aatattttgc tgatttattg tttctaagta
     9901 aagaaattta aattgaaacg attgttattt agggaaaaag gtattttttg aaaaaagaat
     9961 cccgtataat gattgttgtg caaacgctat acaaaatgaa aaagaaaaga gggatgttta
    10021 tggaaaatga acagtcggtc gttttaacga attggaagag aaattatttg ttctttttat
    10081 cgggacagtt tttgtcagga atcactagta tggtcgtcca atatgcgatc atctggtatt
    10141 tgacaagaga aaccggttcg gcgacaattt tgagttttgc tactttacta ggaatgattc
    10201 ccatggtttt actaagccca tttgtaggac cgctagtgga tcgctgggat aaaaaagctc
    10261 ttttgattgt cacggatatt attgtagcga ttttcgcgct tattttagca gtagtcggaa
    10321 cgatttcaga atccttcccg atttggctag tttttgtgtc tttgtttatg cgttcggtcg
    10381 cacaaacatt ccaaatgcca acgatccagt cgattatgcc gacaatcgtt ccatcttctc
    10441 acataacaag gacgaatgga cagttaggaa tggtccaatc agcgaatttc atcattgcgc
    10501 cagctctagg tgcggctttg ttttcagtcg ttcctgtcaa ttaccttatt ttattagatg
    10561 tattgggggc agtttttggt gtcggcttat tgatttttgt gaagatccca aaagtttctc
    10621 ctgaaatatt ggaagttccg cttactattt ttaaagatgc aaaatttggg ctgcagcaat
    10681 tgatggataa taaagggtta tggtacataa cgatcaatgg tgcatttgtg atgctgttgt
    10741 tcatgccagc aatcagtctg tatccattga tgacgctaga ttactttggt ggttccgttg
    10801 gacaggcggg agcagtggaa gtagtctacg cagtcggaat gctcttaggc ggtgcactga
    10861 tcagttttat aggaacatgg aaagaccgaa tgaaacctat tattatagcc tacatcatta
    10921 tgggcctgac gatcggtgca agcggattgg ttccaaacga tagtcaaggg ttcttgtact
    10981 ttcttatctt gaatgcagga gcaggttgtg caacgccata tttcaataca ctgctgatgg
    11041 caatgatcca gcagagttat gaatcgaatg ttttaggtcg tgtcttaggg aacttcaact
    11101 ctttgatgaa cctggcaggt ccaattggat tattatttgc aggaccatta gctgatcgtt
    11161 taggggtaga aaaaatgttc ttgttttccg gaatcgggat tttattatgc ggaatcgttt
    11221 tatttttaac gtcagctgcc cgaaattatg ataaagaatt acagaaaaaa ctggtaaaag
    11281 aacaccatga gcaaaaagac gaatagtatg gaatggagtt gtgacagaag tcttgttaca
    11341 actccatttc tgatcctaat gttcctgtgg caataagcct actcaaatga aaaatatgag
    11401 taattatctt tatttgttta atacttgata tagggcagtt ttttcacgac ctatcgtact
    11461 tcctctttgc ttcatagatt tctttgattt gttgctcaga taacggagtg ccaaatggta
    11521 gtttttcctt taagagttct ctttccggtg cgtctacgcc tacattgaca tcgttctcga
    11581 taggtacgct gtaatgatgg atatgtccat gaagattgcg gatagtgtcg ttaggcccga
    11641 gcaacatcgg ataatgagtc aagtaatatt gttgatggtc gaatttaacg atggcaccta
    11701 catcataaaa ataaaatttg ggacttccgt cagtaagcct aggatcattt ttttctaaat
    11761 aacggaaaaa agcgcgactg tcatggttcc ctttgatcaa gtggattgtt ccttgcagtt
    11821 gagtgaccag ttccaaaatt tctgcatttg ctttttcata ttgcggatgc atagcaaggt
    11881 cgcctagatg ataaacatgg tcggtttctt ttattactgc attccagcta gtgatcaact
    11941 gttggttcat ttcatccaca tttgcaaaga gccgcggagc aaagtcgttt tttcctaata
    12001 agcgttcatg gaaaaagtgc atatcactga taaagtaatt catgtatctt ttgcctccct
    12061 tttttcttag agtaaacaaa atcacttatc tgagcaactc ataacatcta aaaagaaaag
    12121 gttgtaaatg agacgtgcag ctttaagcga taagaagcaa ttttgaaaaa tagtttttca
    12181 caatttataa aattattcct tattgccgaa aggctggccc tttttatgag tatgccaatt
    12241 ttttatatcg aatgctcaaa agatgagtga ttttttggtt gactatcatg tttgtgtact
    12301 gaataggtcc agtagaacag tatttttacg acttgataat taagggatga aattttcaga
    12361 aatatgacgt ataatgtaaa atactgaaaa gttgcgacct tcaactcgta gatgtttcag
    12421 aatctaattg aaatgatggt gatatcatga cagacaacag caaaacacgc gttgtcgtag
    12481 ggatgagcgg cggtgtcgac tcatctgtaa cagcactttt gttaaaggaa caaggatacg
    12541 acgtgatcgg aatattcatg aaaaattggg atgacacgga cgaaaacggt gtatgcacgg
    12601 ccacggaaga ttataaggat gtagcgaaag tagctgctca aatcgggatt ccttattact
    12661 ctgtgaattt tgaaaaagaa tattgggatc gtgtatttga atatttccta gccgaatacc
    12721 gcgcaggacg tacacctaac ccagacgtga tgtgcaacaa agaaatcaaa ttcaaagcat
    12781 tcttggatta tgcaatggat cttggcgccg attatgtagc tactggacat tatgcgcaag
    12841 tcactcgcga tgaaaacggc gtggtccaca tgcttcgtgg agtagacaat aataaagatc
    12901 aaacttactt ccttagccaa ttatcacaag aacagctttc aaaaacgatg ttccctctag
    12961 gcggcatgga aaaatcagaa gtacgggcaa ttgctgaacg cgcaggcctt gcgactgcta
    13021 agaaaaaaga ttcaacggga atctgcttta ttggtgaaaa gaacttcaaa caatttttga
    13081 gcaactatct gccagcaaaa aaaggaaata tggtcactct tgatggtgaa gtcaaaggac
    13141 aacatgctgg cttgatgtat tatacgattg gtcaacgtca aggactaggg atcggtggcg
    13201 gaggagattc gcaagaaccc tggtttgttg taggaaaaga tctagcgaca aacacattat
    13261 atgtggggca agggttccat catcctgcat tgtatgccac aagcttggat gcaagtgaaa
    13321 tccactttac gacgaatgaa ccaatgccaa aagaattcaa gtgtacagcg aaattccgtt
    13381 atcgtcagca agacgttcct gtgacagttc gcttattgga tgataatcgt gcagaagtcg
    13441 tattcgacga acctgtacgt gcaatcactc ctggacaagc agttgtattt tatgatggaa
    13501 tggaatgtct gggtggtggt ttgatcgacc acgcatatca agaaacaaaa gttttacaat
    13561 acgtttaagc gatattaaat gaatcaaaac agctatggct aacaaaagaa acaccttttc
    13621 ttttggatag catagctgtt tttttgcaat aaaattaatt tttattcatt ttatacttga
    13681 aatacttgtg taaaaagttc ataatatcca ataatatgaa taaagttgct ttttgttaag
    13741 caagagcagt tgcaaaatag aagggaatct tttgcaagtg atttgttcaa ttttataagt
    13801 gagggtgatg gaaatggaaa agaaatcgac gggtattagc aaatggggac tggttgctct
    13861 ggttgtcagt tcgtcaatcg gaagcggtgt gtttgggatc acaagtgatc tggctagttc
    13921 tgcagctcca gggccagcga tcctttcatg gataatcgtt gggattggta ttttagcttt
    13981 ggtattatcg ctgaatcatt taggagaaaa gcgtccagat ctagatggtg gtattttcgg
    14041 ttacgcggaa gcttcatttg gtaaattagg tggcttcatc agtggttggg gttactggtt
    14101 gtctgcgtgg ctgggaaatg ttgcttttgc aacgatgctg atgagcgcca tgggagaatt
    14161 tttcccgata ttcaaaggtg gacaaaatgt tccttctatt ttatttgcaa gtatttttat
    14221 ttggggtctg actttattag taaacaatgg agttgaagga gcgagcgtca tcaatacaat
    14281 cgtgacgatc tgtaaattag tgccattatt cctattttta atatttgtat ttatcgcatt
    14341 taaagtgaat atattcactg ctgatttttg gggtaacgta tctgataatc tagtgaatgg
    14401 aacagggcag caaggaacga tctggttgca gattaaaggt tgtttgatgg ttatgatgtg
    14461 ggtctttgta gggatcgaag gcgcaagtgt tttagccaac cgcgcgaaga aacgttcaga
    14521 agcccagcaa gcatcgatca ttggtctgct ttgtttattg tttatctata tccttgcttc
    14581 gatcttgcca tacggcgtct tatctcaaga agagttagca actatttcac aacctgctat
    14641 ggcaaatatt ttaaaaggaa tcgtgggaaa ttggggagca gcgctgatca atatcggctt
    14701 gattatttcg atcattggtt cttggttgtc ttggacgatg ctcccagcag aaacaacgat
    14761 gttgatggct cgagacggtc tgttgccgaa aggttgggga aaaacgaata aaaaaaatgc
    14821 cccaacttat tcgttagttg ttactgcaat cttgactaat ctattcttat tgactttctt
    14881 agttactgat tatgcctatc aatttgccta ttctttatgt acagcggcaa ttttgatttg
    14941 ttatctactt gtcgggatct atcaagtcca gttttcttta aaagaaggaa tcaaaaaaca
    15001 agtagtgatc ggtgggattg ctgtagtttt tgaattgatg ggcatcattt tagcaggatt
    15061 ttcttatgtc cttttatgta gtattgccta tcttcctggt ttttgcttct actggatcgc
    15121 ttgtcgtgaa accaaacatc aaattacggg aaaagaaaaa gtaatgattg ggctgatttg
    15181 tgtggccgca ctgctttcaa tcgggttgct tgctgtaggc tggatcaaga tataaggggg
    15241 aaagaaacat gaaaatcgca aatttcggtg tagaagaatg gctaaataaa tgggaaaaga
    15301 aagcggtata tgatattgca caaagctcga tcgaagcatt gacattagac gaactcgttg
    15361 gcttagatgg gactagcgta agtaaatttt tcgaaaaacg caaaaacgaa ccattggact
    15421 atggctggat cgaaggatca gatgttttca aacaagaagt cgcttctctt tatcaaaccg
    15481 tcgatccgga aaacatctta caaacgaatg gagcgactgg tgctaatctt ttagctctat
    15541 acgcgttagt ggaagcagga gaccatgttg tttcgatgct tcctacttat cagcaattat
    15601 atgacatccc caaatcttta ggtgccgctg ttgactttgt tcatttgaag gaagaagaag
    15661 attggcagtt tgatttagaa cagctagaag ccttagtcaa acctgaaaca aaaatcatct
    15721 gtttgaatag tgcaaacaat cctacaggca cattgctaga tcggaaaact ttagaaaaaa
    15781 tcgcagaaat tgctcgtaca gtagatgcat atgtattgat agatgaagta tatgcgcctt
    15841 taacggataa aggtgaattc ttgtcgatcg tagatgtcta tgaaaaaggt atagcaacca
    15901 attccctatc caaaacctac tccattccag gtattcgtat tggctggaca gctactggac
    15961 ctgagttggc agaagttttt cgaaagtatc gagactacac gatgatttgc ggcggagtcc
    16021 tgtcagatga tcttgctgta catgcattga agaataaaga aaaaatattg gcgcgaaaca
    16081 aaaagatcat tacagagaac cttgtgattt taaaacaatg ggtagccaat gaaccgaaag
    16141 ttgaacttgt cgctcccaat tacgtctcta cttctttcat taaattagcc atcgaagaag
    16201 atgaccagac attttgtatc aatttattag aagaaacagg agtactatta gtaccaggaa
    16261 gtgcctttga tttaccgaag catgcccgac taggttactg ttgtaaaaaa gaaaccttgg
    16321 aaaagggatt attcctctta tctgagttct tgaaaaaaca ggcctaaaca aaaacactgc
    16381 aaggacagtc tgtgacaact gttcaagcag tgtttttcat ttacgcttat tgtctatgag
    16441 aaggagagtg ccctgaagct atggagactc ctgaagcttt ttcttctgca gtttcttgct
    16501 gatggatctc ttgtgtattg tgtcgtacaa gcaagaagtt gaccaaaaca agaagagaag
    16561 tcgaaagaaa gacgccgcga taaccaaatg cactggaaac actggagccg atcataggcc
    16621 ctactacgtt accagttgct tggaaagatt ggttataact gaagatccgt ccagcagctt
    16681 gatgaggcga atattttgtg atcaatgctt ggacagcagg gagcagacaa gcatcagaga
    16741 taccaatcaa aaaacgtaag gcagctaatt gccaaacatt cgtgacgaat gccattggga
    16801 tataaaccag aatcgcaaaa cccaaaccaa tcgcaagtat tctttcactc ccaatcttgt
    16861 cacctaagcg accaaatctt ggagcggcta tcaaagtggc aatcccaggt atcgaagcaa
    16921 tgattccgct gatcaacgta acattcccat gtccatgcag caattggcga atgtacagac
    16981 tgatgattgg actaatggaa ttgttggatg cttggatgat catcgttgtg acgaacatac
    17041 cgatgacgac gtgtggatat ttcaagtctt tgaaaatctg tttggcagaa accatgtttt
    17101 cttttttcac tgggacaaat ttttcatgga cgaaaaacag actcaacaag aagacgaaaa
    17161 ataagatgat ccctgtaatg aaaaaggtag ggcgatagcc aaacgctgaa gcagagatcc
    17221 cgccaagaag cggtccaagt aatgttcctg taacagaacc agttgctaag gttccaagaa
    17281 cttgaccact tttttctttt ggcgtcccag ttgcgacaag ggcagtggca ttactgatat
    17341 aaccagaaaa aatcccttga agcagacgca atgcgactaa ttgatacaca ctagtcacgc
    17401 aacccatcaa agaaatgacg attgccattc ctaatgaagc acgcaacagc atcaattttc
    17461 ttcctttttg gtcggctaat tttccccacc aaggagagat aagcgtagtc accaagaaag
    17521 tagatgaaaa agtcaatccg ctccaaaagt tcaattgtga cgtactataa ttacctaacg
    17581 tatctatgta aagtgacata aaaggcatga ctaaactaaa tccgatacca gccataaatg
    17641 ttccaaacca taagacaaac aagttttgtt cccaagtttc tcttttccta aataattttt
    17701 cctttattct tgcagccaat tagactcctt ctttctgaaa attttcatct acagctcttt
    17761 attataaact acaatatgta gtttatcaat gaaagtctct tttttctctt tctttttcgt
    17821 ggtaaactga aagagtgaag gatttatttt atgagaggaa acatggaaat gaaaaaaaga
    17881 aatctgtctc aagagaaaat catcgactgc tttcgagaac tagcagaaga gatggatgtt
    17941 cagcaaataa cctttcagca tttagccaaa cgcttggata tcaaggctcc ctcgctgtat
    18001 aaccatttca aaaatattcg tgatgtcaaa acagcgctaa cagcgaaact tctgcaggaa
    18061 cttaatgacc agctgcgacg ggctcttgtg ggaaaaagtg gtcaggaagc actgcagatc
    18121 tatgcagaaa cttatcgatc ctttgctttt tcaaatcaag cagtgtatga gttactgatc
    18181 agtgtaccgc ataccaatga agctgtttta ttagatggaa tccatgaaac gaatcagatc
    18241 attttgcaga tatttgaggc ttttcaatta acgaaagaag aaaaagttca tcgaagcagg
    18301 gaacttcgta gtatgatcca cggttatctt tccttacgtt ttttaggata ctttacaaaa
    18361 gaaccaacaa tcgatccaga agacagctat ttttggatga tcaataactt tatcgctact
    18421 ttgccttaaa caatcagatt catttgtaac aattttaaga tgttcattaa atgacccttt
    18481 tataagagaa aattcttgca actttcttga aaagtgacta agatgagtct agaaaagctg
    18541 aaagaaggag cacgagtatg tatcaagtaa ttaccatgtt cggggacaat gagccttggt
    18601 ggttttttga agactgggaa gaagatattg aagaagaaga aacattcgag tctttcgaag
    18661 aagcacgaca agcgtacgaa aaacaatgga acgaaatcca tcaaaattat gaatatatca
    18721 atgcaaaaag taactttctc agcgcctttt ggaatgacgg agatgagcgc tggtgtgaag
    18781 agtgtgacga tgatttacag caatacaagg gacttgcgtt gttgaaaaat catcagccca
    18841 tcacagtaga aagcagaaag gaattttatg aaacaactaa ttctagcgga aaaaccaagc
    18901 gttgcgaaag acctaagcaa ggtgcttggt gctaatcaaa agcacaaaaa ttattatgaa
    18961 ggacccaagg tgatcgttac ttgggccctt ggtcatttat taggattgaa aatgccggaa
    19021 gatctgaata aggaatggca gagctggcaa atggacacgt tgccaatgat ccctaaaaaa
    19081 ctaggaatca aaccacttcc taaaacagga catcaattaa aagcaatcaa gcagctggct
    19141 cagcgaaaag atgtctcaga agcagtcatt gcgacagatg ccggaagaga aggggaactt
    19201 gttgctcgct ggatcttaga gtatgtgcat ttcaataaac cagtgaagcg gttatggatc
    19261 tcttctcaaa cagataaagc aatcaaaact ggattcaagc aactaagacc tgcgaaagaa
    19321 tatgatgcat tgtatgaatc tgctcttgcc cgggcaaaag cggactggct ggtaggattg
    19381 aatgtgacca gagccttaac tgtgaagtac gcggataatt tatcagcagg ccgtgtgcag
    19441 acaccgacac tagcattagt gagagaccaa gaaaagaaaa tcgagcagtt ccgtccacaa
    19501 acgtatttca cgatcacgtt gcaagctgga aaagaacaag ccaaaatgct tcaaaaaaat
    19561 caatttgctt taaaaacaca agaagaagca caaaaacttg ttcaattgat cagtacaagc
    19621 aaaggaactg tcacagaagt ccaagaaaaa ttaaaaacgg aatctgcgcc attgccttat
    19681 gatttaacag agatccaaag agaagccaat caacgctatg gctattctgc aaagaaaaca
    19741 ttaagtcttg tacaaagttt gtatgagacc cataagatcg ttacttatcc gcgtacagac
    19801 agcaaatatc tgacaaatga catgaaaagt acgatgaaag aacgattgca agcagtcagc
    19861 gactttgctc ctgaggtcaa gcagtatttg aaaaacggag ccatcgtcca acagacaaaa
    19921 gtattccaag acagtaaagt gaccgatcac catgcacttt tgccaactga aaacagagca
    19981 cgttatgaaa aattaagtaa tgaagagcga aaaatctatc aaatgattgt ctctcgtttt
    20041 ttaggactgt ttgctcaacc ccataaagtc agccaaacaa aagttacggt tgaatttggt
    20101 aaagaacgat tcatttttcg gcaaaatcgt gtcgtccaag caggatggaa aggcgaaact
    20161 gaaactgaga cggaaacagt caaatgggaa aaaggaatga gaatcaatcc cgatttcgcc
    20221 atcaaaaaag aactatctgc accgcctaaa ccattgtcag aagcaagctt gcttggtcaa
    20281 atggaaaaat acagtctggg aaccccagct acgcgtgcag agattatcga gaaactaatc
    20341 aaatctgaac tgatggaacg tacccctcaa ggactgcaag tatctcctaa aggtaagcaa
    20401 ttattagtat tggtaaatcc ctctttagta acaccagatt tgacagaaag ctgggaaaaa
    20461 gaccttgaag ctattgctgc aggaaagaaa caagcaggaa ccttcttgaa tggaatcgaa
    20521 aaagaaacaa aacgcttggt gaatgagatc aaatcaagca aacaagaata tcaagatttc
    20581 tcgattaccc aaaagaaatg cccagaatgt ggggccaatc tgcgcgagaa aaacacacgt
    20641 gacggcaaaa tctatgtctg caccaattca gattgcaact accgcagaag aaaagatcca
    20701 aaagtctcta atcatcgatg cccgcaatgt caccggaaaa tggaaatcat cgaaggtaaa
    20761 aacggtgctt atttccgttg taaatacgat ggaacgacag aaaaattgct tgataaaaaa
    20821 gagcagaaga agaaaatgac caaacatgaa gaacgacgct tgctgaaaaa atatgcacaa
    20881 gcagaagaac cagaagaaag cccattagca gcagcactaa aagcagcaat gaagaagtaa
    20941 taagatatga aagaggttgt gatagaagtg gtcagcttca agaaataaga aggaatttac
    21001 gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt tccgaagaag ctacttctgg
    21061 aacaccattt attcggaaaa aaggactgtg acaagacttt tgtcacagtc ctttttcatt
    21121 taaaaaaatg tttacttcat ttgacgtata ttattggtga ggcttcaatt catttggctt
    21181 cactttttct gtttctctta tgatataaac aggacgctgt ttcgtttcta agaagatttt
    21241 cccaatatat ttcccgatga ttccaagaca taacaattgg attccaccaa tgaacaggaa
    21301 gatcgagacc atggaaggcc atcctgatgt aggatcacca aacaacaatg cccgcaaaat
    21361 aatgaacaaa attgcaatac cagaaccgac acaagacaaa gcgccgataa atgaagcaat
    21421 cgttaacggt gcatccgaga agttcacgat accatcaatc gagtagttga ataatttcca
    21481 gaaggaccaa gatgtttcgc cagccacacg ttcgcggttt tcataagaaa tatattctgt
    21541 tttaaatccg acccagctaa ataatccttt tgaaaatcgg ttgtattctg ttaattctag
    21601 gacggcatcc accatttgac gagtcatcaa gcggaaatca cgagcgccat cgaccatttc
    21661 tgtatcgctg attttattga tcaactggta gaattttttc gcaaagaaac tgcgtatcgc
    21721 aggctcgcct tcacgagtcg aacgccgggt gccgacacaa tcgatatcag aagtagtgat
    21781 ccggttgatc atctcaggaa gcagttcggg cggatcttgc agatcaacat ccatcacggt
    21841 cactaaatct cctgtagaag cttgcaatcc agcgtacaga gccgcttctt tcccaaaatt
    21901 tctggaaaat gagacaaagc ggacataagg ataagctgag gctagcttct tgattgctgc
    21961 taacgtaccg tcttttgatc cgtcgtcaat aaacaaatat tcgaactcat gggcaccttt
    22021 gaatttttca acctcatcaa aaaaaagagg aacagttttt tcttcattaa aacatgggac
    22081 aacaattgat actttcatcg tttctatcct ttcaatcgta tgtttatata tggtttacat
    22141 tcaaaattta ataaacctta aataacacct ttaaaatttg attttacgta aaagaacagg
    22201 tattcttttt gtaaactaac gtatttagtt taccatgttt cctcggttta tcaaactttt
    22261 aagaacaatt tttattagtt ggcaagtagt tttggataga gaaaaaggag gaagttccag
    22321 tctattgact aggagcaaac aaaatgaaaa tacacacacc tatcggcttt ttatggttag
    22381 atgcagatga atccggattg actgctgtat cttttcagcc aatttcagaa agcgaatcag
    22441 cgaatcagcg aattttaacg cgcacacaag aacagtttga agaatatttt gcgggaaaaa
    22501 gaaaagaatt tgatgttcct ttatctataa agagaggtac gccttttcag caaaatgtct
    22561 ggaaagcttt gtcgaccatt ccttatggag aaatccgaac gtataaagaa gtagccgttg
    22621 ctgttgatag tccgaaagct gtgcgagcga ttgggcaggc aaatcgaacc aatccattgc
    22681 caatcgttat tccttgtcat cgagtgatcg ggcaaaatgg aaaactgaca ggatatatgg
    22741 gaaacgcaat agatggggtt gaaatcaaac gccaattgtt gttgttagaa ggatatctgc
    22801 aaaattaaaa acagttgtga ttcggacatt gttccatgcg agaatcacaa ctgttttttc
    22861 atgttcattc taaactttga tcagccaaat aaatagtaaa gccatcactg tttggagaag
    22921 cccgacagct aatccataag ataagaagcg tgtaccttct ttcatgaatt ttgcaaagtc
    22981 taaacgcaga ccaattgccg ctaaagcagt gatctcaaac cagctgctga taaaatgtgc
    23041 cgattggtca ataactacag gtaaagaaaa caagctgttg accaaacaaa agatcacaaa
    23101 ccctaataca taccaaggaa gcggtaattt tcgttttttc tgcgtatcag ttggcgtttc
    23161 aaacagagct ggttgtttct tgtgcatgaa tttttcaaac agatagacaa caacaacgag
    23221 catcataata cgtagaatct taaatagcat ggcgaattcg acaacagaat tgtcaagtaa
    23281 gcttgcccca gcgactactt gaccaacaga ttggagtgta ccacccaaaa gtgcactttt
    23341 ttcaatgaga tttccaccaa acaactgtaa tccaagaaaa gggagagtca acatcatcac
    23401 agtacccagt agattgacta gtgtgatgat ctgtcctttt tcttcctctt tcgcatggat
    23461 ggaaggggcg attgaagcaa tagcagaaga accacaaaca gcgtttcctc cagccatcat
    23521 caaagaagaa gtatcgctga attgcagttt tttaccaatc aagtaagcac cgatgatagt
    23581 gactgacatt tgcagtaaca caaaaacaat tccttttaca ccaatttgag caatcgtttg
    23641 agctgtgaca gttgcaccta gtaaaaaaac agagaattct aatagtttgc tttcagcaaa
    23701 ttttgtgccg attgctagaa caggctgttt aaaaaaagta tttcctaata aaatacccaa
    23761 taaaatagca atagtcgctc cacctaactg tggcagataa atagcaagaa gcctgctgaa
    23821 ggcagcgatg gcaaaagaaa gcagtaaacc gggtaagatt tcgcggaccg ctgaatcctt
    23881 tacagatttt attggatgtg acaatgattt catttgatcg gttcctttcg tcaataaata
    23941 ctaaaagcat actcgaacat agtagcacta aaccagtatt ttgacaaaag aacaagaggt
    24001 cgtgacaaaa gtcttgtacg acctcttgtt tcgaataaac ggtgttccag aagtagcttc
    24061 tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
    24121 atttcttgaa gctgaccact tcttttacga cctcttggta aacggtgttc aaaagcttgc
    24181 atctgcggta ataagcccaa ccagtacaaa aacatgaaca gctgtttttg tttggccgtt
    24241 cttattactt gatgcagaac agctttttca caacctattg aatcgattat taagcgattt
    24301 ttttagaaac aaattttcca tgtttgctat gttctttaat taagaaacgt acgtaatgag
    24361 caagaacgta gaagacaact acacttgcaa acagacattc cgcaactttt aagaaatcca
    24421 atacgctact atagatacct aaactgttga agtaatgaac agtagtaaat atagctgttg
    24481 cagagataac aagcgggaat gtaaaggctg catagcttgg ataaaaacga agcttgaaca
    24541 aacgtggaag catagacagc acaacgaaat acaatacttg agataaagcg aacaatgcta
    24601 cgacaagata aaaattaggt tcagcaaagt ttttaagata acctgttagg caaagagaac
    24661 caggtgctgc aacgatcgta atcagcggac gtgtcggatg agccatattt cttacgaaaa
    24721 agacacgttt gatgatgatc ggcaataaga acgcatagca gatcaatgaa atccaaaaga
    24781 aaatttttcc aataagtggg aagaagttcc cggcagttac agtgatgacc ccaaatccaa
    24841 cgtaaatgat aaaccagctt ggataaattg cttcaattga aagatcagct ttgataacat
    24901 ggtgataatt gaaataaaag acaaggatca cttggaagat aacagcgcac agccaaatga
    24961 atttgacaat tggtgcaata ctttcatatg aaacaaaata agtacaaata accatgagtg
    25021 acatcgtaaa agttggtgaa acggaggcga tgattggatc atgcaacgtt tgtttcgtgt
    25081 gttcgaataa gataaacaat ttaacaaaaa tgaatatcat aagaatcata gagataccgc
    25141 cagttagatt gcctaaagca acatggtgat agtctttaaa cagatttcca agggaagcta
    25201 acccaaggat caacccgcag atcgggatcg gaataatttt caaaaagttc ttcatatata
    25261 atattctcct ttttatttaa tagattttag taccaagatg aacgaataca ttgtaaaatg
    25321 aataggaatg tatgattacg agcaagcaga cagctgattg tcttcatcta agttactagt
    25381 tggatcagca actgttttgt tgtacatttc tattatagat ggttttttgt atatttaaat
    25441 caaatagttt ttatagtaat tatcataaaa agttatggaa gtggtgccat gtttaagtta
    25501 ttaacgactt ttcgagcagt atatgaaaca agaaattttt ccagagcagc agaagtactg
    25561 tttttatctc aaccagcagt ctcgaatcaa atcaaacagt tggagcggga attgaatatc
    25621 gatctttttc aacgtaacgg aagaaaagag atgcttccaa ctaagcaagc cgacttatta
    25681 tatgaacgga cactggtttt gttagaagaa tgggaagaaa ctcagcaaaa attgctcaat
    25741 gaacaaaatg aagaagaagt atgtcggatc gttgcttccc acacctttgc tgtctatctt
    25801 ttgcctcagt tgatgaaaca tctgatcaaa aggtttccta aagtgcattt tgagatcgaa
    25861 atcacgaatt ctcatgaatc attagaacaa gtagcgaaac atgaatccga ttttggtttt
    25921 atcgaaaagc cattagagac ttccggcgta caaagatact ctttgatgga agaccagcta
    25981 gtcatcgctg gggacccaaa aagcaaattg tggcttgtaa gagaagcaaa ctctggtgtt
    26041 tttcattaca cgcagcgtta ttttgaagag atgaatatcc aagataagaa gatgatcgtg
    26101 aaaagtaacg atgtaattgt tgcgttgctg aaggaaggaa tcggtcgctc gattttgtct
    26161 aagcgctctg ttccagaaaa tgtgccaatg actccgttga atttccatcg atatttttat
    26221 ttcatccaac gaaccaatct ggtttctgat cagctgctag agattgccga agagatcctt
    26281 cgatacaatg aaagcacaga aacttctcac agcgaggaga atctcagctg gtagataatt
    26341 gcatgtatca aaaaaagttc tatgatacac taaagataag cagtaggctt ttgtactgca
    26401 tttgctcaag caactgcaca aacggaaagt taatttttcc caaatgtcta tggaaagact
    26461 ttccattatc taactggacc gctataataa tcagcagtat ggtttttaag aaaagggatg
    26521 aaacatggaa aaaaaattaa aagcttcgaa cgataatcgt attgattatg gcgtgatttt
    26581 accagtcttt ctgttatgtt tgattggtct gctctcatta tatgtagcac ttagccatga
    26641 tccgaacaat ccagcagtct tgaaaggcgt agggatgcaa gttgtctggt atttagttgg
    26701 agcggcagca atcgttgtca tcatgcattt gaattccaaa tggatctgga aactgactcc
    26761 ttacctttat ggtttggggt tagtggtaat gggactcttg ctcaaattct atgatcagaa
    26821 tttagctgct tcaacagggt ctaaaaactg gtttcgattc ggtgcgttta ctttccagcc
    26881 agcagagttg atgaagattg cgtacatctt gatgatggca ctgatcgtaa ctaaacataa
    26941 tacgcaagta aaagaacgga cgatgaaatc ggacttttgg ctgattggga agctcttgat
    27001 cgtgacaata ccggttttag cattgatcat ggcccaagat gactttggaa cgatgcttgt
    27061 ctttcttgcg atctttggcg ggattttcct gatgtctgga atctcttggc gcatcatcgc
    27121 acctgtagta atcttggcag tagtcgttgg agcaggaacg atcttcttgg ttacgactga
    27181 aggcggaaga gacttgcttt ataaagttgg attcaaatct tatcagtttg cacgtattga
    27241 ttcatggctg gaccctttcc atgatacgtc aggaatgagt tatcagccgg cccaaggact
    27301 tttggcaatt ggtaccggtg gcttgtttgg taaaggattc aacgtaagca atatctatgt
    27361 gcctgttcgt gagtccgata tgatttttac cgttatcggt gaaaacttcg gctttatcgg
    27421 tggtgcattc gtcattttct tgtattttat tttaatctat cgtatgattc gggtgtgttt
    27481 cgatacgaat aatgaatttt acgcctatat cgcaagcggt ttgatcatga tgctgctgtt
    27541 ccatgtcttt gagaatattg gagcaaatat cggattactt cctttaacag ggatcccatt
    27601 gcctttcatt agtcagggag gatcctcgat tttagggaac atgatcggta tcgggttgat
    27661 attatcgatg agataccaaa atgaagcacc tgcaagatct tcaaggaggt aagaaaattg
    27721 tacacctttt actggtaccc gaaatgttca acatgcaaaa aagccaaagc gtggttagac
    27781 cagcatggcg ttagttatca aaccgttgat atgatcaaag aagtaccgaa ggcctcaacg
    27841 attgagactt ggctagaaac gagtcaagta ccaatccgtc gcctgttcaa cacaagcggg
    27901 atgaaatatc gggaacttgg actgaaagat caagtaggcg agtttacggc ttcaaaagcg
    27961 agccaagtct tatcgacaga tggtatgctg atcaagcgac cactgatctt acgagaggac
    28021 cagcttgttg caattggatt caatgaaaaa atttatgaag gagaactgat ataaaatggc
    28081 aggcaagcat ttgaagaaaa aggacaacct ttggatctta tttaatggaa aagaatactg
    28141 tgttggtttg acaaatgaag cgcaagaaga acttggagac atcacgtttg ccaatctgcc
    28201 aaaagcagga caatcgctaa aaaaagatga acctctgatc gaagttgaag cggaaaaagc
    28261 ggtcaatgaa tttgccagcc cgctaacggg tacagtttct tctgttaatg ataaaatcgg
    28321 tgaagatatc aatatcttga atgattcaga tgaaatgaat gcgtggatct taagtttcaa
    28381 agatgttgat ccaaaagaat ttgacgaatt ataaaatcag aaaatcactt gaaaaaagtt
    28441 gacactgatt cttccagttg ctataatcat aagcaatcct atatgaaacg agaaagcttt
    28501 gaaaagaaga gtacatgtca cggacgtttg aagagagccc tgtttgctga aaatgggtaa
    28561 cgaaaatgag atggaagatg gtcttagagc agagtaagcg agcgtaagca aacttactac
    28621 gggagtgccc gttacagcgc gacagtatac ggaaagaagc taagtccaag tactggaaga
    28681 ggctgttatt gcgaagtaac ggtaaatcaa ggtggtaaca cggaagttca agctttcgtc
    28741 cttttgctta aggcaagagg gcgaaggctt ttttgtttga aaaattataa gagaaagatg
    28801 tgaatggcat gtcattaatc caactaacga atgtcaaaaa gacattcaat gggaaaaacg
    28861 gaaacatcca agccgtcaat gacgtttcac taaacgttga aaaaggggat atttatggaa
    28921 tcgtcggcta ttctggcgca ggaaaaagta cattagtccg tctcctgaat ggacttgagc
    28981 ttcccacgag cggcgaagtg atcgtaaacg atcaagatat tacaaaatta aaaaacaaag
    29041 aattacgtac attccgaaag aaaatcggta tgatcttcca gcatttcaac ctgttatggt
    29101 caagaactgt acttgaaaat atccagctgc cgctggaatt agctggcgta ccaaagagca
    29161 aacgtaaaga acgggcagaa gaactgttgc gtttagtcgg gttagaagga cgcggacaag
    29221 catatccttc acaactttcc ggcggacaga aacaacgcgt cggtatcgca cgagcattgg
    29281 caaatgatcc tgagatcctg ctttgtgatg aggctactag cgcattggac ccgcaaacaa
    29341 cagaagaagt attagactta ttgttagcga tcaataagaa attgaatttg accatcgtct
    29401 tgatcacaca tgaaatgaat gtgatccgca aaatctgcaa caaagtcgct gtgatggagt
    29461 tggggaaagt cgtagaagaa ggagacgtat tgactgtctt cagacaaccg aaaaaagaag
    29521 taaccaaacg ctttgtacaa caagacatcg aaccagaagc tgattcgaca gaactattag
    29581 ctgaactcat caaagaaaat ccaagcggcc gtttggcaac tttgaccttt agcgaaagaa
    29641 atgccaacga gccagtcatt tcccaagcga ttcgacgcta taacatcgat gtcaacgttg
    29701 tgtatggaaa gatcaaacaa gtgaaagaag gttcctttgg ttcattgact gtcttgatga
    29761 ctggtgaaac caaagaatta gatcaagcag aagtgttcat caaagaacaa ggagtaggaa
    29821 ttgaggtgat ccatcgtgga taaaagcttt tcagaaacat atctagattt taaacaaatc
    29881 aacccggaaa cgatgaatca ggctatcctt gatacactat atatgacatt aatctcaatg
    29941 gtcttagtga cgatcatcgg attagttttg ggattgatcc tttactctat tggacgcaaa
    30001 aacaaaccgt atgctcgaat catttatgga atcgtatcga tcatcagtaa tattttccgg
    30061 tcaacgccat ttatgatcct gatggttttg atcattccat ttactaaagc aatcgtaggc
    30121 actatgctag gtgcgcaagc agccattcca gctcttgttt tatccgcagc accattttat
    30181 gcccgtctag tagagattgc ttttcgtgaa gtcagccctg gtgtactgga agcagcagat
    30241 gcaatgggag caagttactg ggaaatcatc tggaaggtct tgatacctga aagtattccc
    30301 gcattgattt ctggattaac agttacaacg atctcgatga tcggatttac tgcgatggcc
    30361 ggcgcgatcg gcgcaggtgg acttggaggt cttgcttggc aggaaggcta ccagcgtggt
    30421 aatctaacag tgacattcgt tgcgacttta attattttac taatcgtatt tatcgttcaa
    30481 ggaatcggtg atttcttaac taagaaaaca gacaaaagat aataggatat agggggatgt
    30541 aggatatgaa aaagaaaatt tttggatttg cagcagcgct tttgctgact gtaggattag
    30601 cagcatgcgg taacaacagc gacagcaaag attcagcaaa taaagcagac gacacgacat
    30661 tgaaagtcgg cgcttcaccg acaccgcacg cagagatttt ggaacatgtg aaaccattat
    30721 tgaaagatga aggaatcgat cttgaaatcg tcaaattcga tgactatgta ttaccgaacc
    30781 aatctttgga agaaggcgac attgatgcca attacttcca gcatattcct tatttgaaca
    30841 aagaaatcaa agaaaaagga tatgactttg tcaatgccgg tgctgtccac atcgaaccaa
    30901 tgggcttgta ctctaaaaaa gtcaaagatg tttccgaatt aaaagatggt gcaacaatca
    30961 ttacttcaaa ttctgaatca gactggggac gcatcatcac aattctacaa gatgctggct
    31021 tagtcaaagt caaagatgga gtagatctgg aaacagcaac attcgatgat attgaagaaa
    31081 accctaagaa cttgaagttc aaccatacga tcgacccatc acttttagct tctacgtacc
    31141 aaaatgatga aggtgaccta gttgcaatca acgcaaactt cgcttacggc gcagggttga
    31201 atccattaga agatgccgta ttgcttgaaa aggacaactc tccttacgtg aatattattg
    31261 cgactcgtaa aggtgatgaa gacagtgaca aaatcaaaaa actgattgaa gtgcttcacc
    31321 gcaaagaagt ccaagaatgg atcacagaca aatggggcgg ctctgttaaa ccagtaaagg
    31381 ctgatgcaaa ataaggtatg gaacaaaaaa gatgagaaat aagtctgtaa tggcttattt
    31441 ctcatctttt ttatacctat tctaatttag aaatcaaaat tgtttaataa taaataaaag
    31501 atatttgatt ttagtaagga aggtcattgc gcttcattct caattagtct aaaatgagtg
    31561 agaacgaaag agaatttggg ggtataatgc atgtctgtct tagaaataaa aaatctacat
    31621 gtgtcgatcg aggaaaaaga aatccttaaa ggcgtaaacc taacaatgaa aaccggagaa
    31681 atccatgcca tcatgggacc taacggcaca ggaaaatcta ccttatctgc cgctatcatg
    31741 ggcaatccaa actatgaagt aacagaaggc gaaattttgt ttgacgggaa aaatgtttta
    31801 gacttggaag tagatgaacg tgcgcgactt ggcttatttt tagctatgca atatccaagt
    31861 gagataccag gtatcacgaa tgcggaattc atgcgtgcag cgatcaatgc gaaacgtgat
    31921 gaaaacgaca aaatttctgt tatggcattt ttgaaaaagt tagatgaaaa aatggcttta
    31981 ctaaatatgc cagaagaaat ggctgaacgt tatctgaacg aaggtttttc tggcggagaa
    32041 aagaaacgga atgagatcct gcaattgttg atgttagaac ctacatttgc aattttagac
    32101 gaaatcgatt caggtctgga tatcgatgct ttgaaagtcg ttgctaaagg agtaaatgaa
    32161 atgcgcggag acaatttcgg tgcattgatc atcacccact atcaacgatt attgaactat
    32221 atcacacctg atgtcgttca catcatgatg gaaggccgag tagtcatgac agggaatgct
    32281 gacctagcta aacgtctgga agcagaaggc tacgctggta tcagtaaaga attaggaatc
    32341 gactataaag aagaagaagc gtaaggggga gcaagaatga aagaaaaaaa ctggctcgat
    32401 tatcttgacg cggtaaatga tttttcttta agtaaaggcg aacctgattg gatgagaacc
    32461 ttccgtcaag acgcactagc gaaagcagat gaactgccat tgccgcatat cgatcgggtg
    32521 aaattccatc gctggtcgtt atttgatgtg aaagaaacac aaaccatcag tgaaacaggc
    32581 acgattccgg catttgatgc aatgaaagac aatcctgtgc ttgtccaaca aggttcatgg
    32641 acgatttttg aacaactgcc tgtggaatta gcagaaaaag gagtcatttt taccgacttg
    32701 ttcactgcaa tgatcgaata tccagaacta gtccaagaat attatatgaa aaaagcagta
    32761 aacatgaacg aagaccaatt gactgcactc catgtagctt ttatgaacag cggtattttc
    32821 ctgtatgtgc ctaaaaatgt cgtgatcgat gaaccactcg agtccttatt cattcaagat
    32881 ggcgcatccg atgagcactt cttcaagcat gtgttgatcg tggcagatga acatagtgaa
    32941 ttttcttatc tggaacgttt ccagacaacc aaagaacaag tagcaaaatc atcaggtaat
    33001 atcatcgtgg aagtcatcgc taaagctggt tcaaaaatca aatattcagc cgtagatcaa
    33061 ttaggagaaa atattacttc ttatatgaat cgccgtgggc acattttacg agatgcttct
    33121 gtcgactggg caattggtgt catgaatgac ggtcatgtga tcgctgactt tgactcagat
    33181 ttagcaggag aaggtgcaca cgcagaagtc aaaatcgtgg caatcagctc gggcagacaa
    33241 atccaaggaa tcgatacacg agtaaccaat aaagcccctc atacgatcgg gcatattttg
    33301 caacacggag tgatccgaga aaaaggaaca ttgaccttta atggtatcgg ccacatcttg
    33361 aaaggggcaa aaggtgcaga tgcccaacaa gaaagtcgtg tcttgatgct ttctgataaa
    33421 gcacgtggtg atgcgaatcc tattcttttg atcgatgaaa acgaagtaac tgctggacat
    33481 gctgccagtg taggacgagt tgatccagaa gaaatgtact atctgatgag tagaggattg
    33541 cataaagaag aagcagagcg cttagtgatt cgaggattcc tcggttctgt cttaacagca
    33601 atccctgtag aacaagttcg gaaagaatta gtcgaagtaa tcgaagggaa gttgaatgga
    33661 tgatcgatgc taaaaagcta cgcaatgatt ttcctatctt atttcaagac gtcaatgatg
    33721 aacctttagt ttatctggat aatgcagcga cgacccaaaa gccaaaacaa gtactccagg
    33781 cattagcgta ctattacgaa cacgataacg ccaatgttca tcgcggtgtc catacgttag
    33841 cagaacgcgc gacccatgat tatgaacaag caagagaaaa agtacgggcg ttcatccatg
    33901 caaaagaaac agcagaagtg ctgtttactc gtggtacgac gacaagcttg aattgggtag
    33961 ccagaacctt cggcgaccgc tatatcaaag caggagacga gatagtgctc tcttacatgg
    34021 aacatcattc taatgttatc ccgtggcagc agttggcaaa acgaacaggt gctgtcttga
    34081 aatatatcga actaacagaa gaaggcttcc tggatatgga ggatgcagcg aaaaagatta
    34141 cggacaaaac caaaatcgtc tccattgctc atgtatccaa tgtcttaggt gtcttcaatc
    34201 ctgttcaaga attagctgaa cttgtccatg aaaacggcgg ggtcatcgta gtagatggtg
    34261 ctcaatctgc cccgcacatg gccattgatg tccaagcact agactgtgat ttctttgctt
    34321 tcagcggaca caaaatgtgc ggaccaacag gtattggtgt tttatatggt aaacgaaaat
    34381 ggttagaaga aatggaacct gttgaatttg gtggcgaaat gatcgatttt gtcgaattat
    34441 acgacagtac atggaaagaa ctaccttgga aattcgaagc agggacacca aatatcgccg
    34501 gcgctatcgc attaggccat gcaatcgatt atttgcaaaa actcggtatg gacaatatcc
    34561 atcgatatga agaagaacta gccgcttatg tgttgccaaa attacaagca attgatggat
    34621 tgactgttta tggtccacaa gacccgaatt ttcggacagg agtcgttgca ttcaatttgg
    34681 atgggttaca tccccatgat gtcgcgacag ctttagacat ggaaggggta gccgtcagag
    34741 caggtcatca ttgtgcgcaa ccgctattga agtaccttga agtatcagcg actgctcgcg
    34801 cgagctttta tttctacaac acaacgcaag acgccgaccg tttagttgaa gcaatcatgg
    34861 caacaaagga gtttttccaa catgggacta tctaaattag acaatttata ccgtcaagtg
    34921 atcttggatc attcttccca tccgcatcat cgtggaagct tgactgactc gacaagcaag
    34981 atcgaattga acaaccccac atgtggagat gtgatcgaat tgcaagtcga tgtaaaagac
    35041 ggaatcatca aaaatatcgc ctttgacggc agtggctgct ccatcagtac ggcaagtgcc
    35101 tcgatgatga cagacgccgt catcggaaaa tcaatagaag aagcagaaca tttaactgta
    35161 gactttctac agcttgtcca aggaaaagaa gtcaaagatc ccgaccaatt aggcgatgct
    35221 gctatgcttg gtggtgtagc taaatttccc gctcggatca aatgtgcgac acttgcctgg
    35281 aaagcactag gaaaagcaat ggaaaacgat ggtaccgcag ctttccatca ttaccacaag
    35341 gaagaggagt gagaacatgg gcgtaccaga attagaagaa tataaattcg ggtttcacga
    35401 tgatgtcgaa ccggttttca gtacaggaga aggcttgaca gaagaagtga tccgtgagat
    35461 gtcaagaatc aaaggcgaac cagaatggat gcttgaattc cgtctgaaat ctctagaaac
    35521 atttaacaaa atgccaatgc aaacatgggg ccccgacctt tcagacatcg atttcgatgc
    35581 catcaaatat taccaaaaac caagtgacaa acccgctcgt gactgggaag acgtaccaga
    35641 aaaaatcaaa gatacatttg aaaagattgg tataccagaa gctgaacgag cttatctagc
    35701 cggtgcttcc gcacaatatg aatcagaagt cgtgtaccat aatatgaaag atgaattcga
    35761 aaagttagga atcgtcttta ctgacactga ctctgctttg aaagaatacc ctgaattatt
    35821 caaagagtac ttttcaaaac ttgtaccgcc aaccgacaac aaattagcag cgctgaattc
    35881 agccgtatgg tcaggtggga cctttattta cgtgccaaaa ggcgtcaaag tcgatgttcc
    35941 attacaaacg tacttccgga tcaatgcgga aaacacgggg caattcgaac gtacgctgat
    36001 cattgttgac gaaggagcaa gcatccacta tgtcgaaggt tgtacggcac ctacttattc
    36061 aagtaacagc ctgcatgccg ccatcgtaga aatctttaca agaaaagatg cgtattgccg
    36121 ttacacaacg atccaaaact ggtcagacaa tgtgtacaac ttagtaacca aacgagcaaa
    36181 agcatacgaa ggcgcaaccg ttgaatggat cgacggaaac ctaggtgcaa aaacaacgat
    36241 gaaataccca agtgtttact tagatggaaa aggtgcacga ggcaccatgc tctccatcgc
    36301 ctttgcagga gcaaaccaaa tccaagatac gggtgcaaaa atgatccaca atgcaccaaa
    36361 tacatctagc tctatcgtct ctaaatcaat ttcaaaagac ggcggagaag taaactatcg
    36421 aggacaagta acctttgcca aaaacagtgc gggttcgatc tctcatatcg aatgtgacac
    36481 gatcatcatg gacgaatggt cgaaatccga tacgatccca ttcaatgaga tccacaacag
    36541 ccaagtctct ttagaacacg aagccaaagt ctcaaaaatc tcagaagaac aactctacta
    36601 tctgatgagc cgaggcctga ccgaatctga agcaactgaa atgatcgtca tgggctttgt
    36661 tgaaccattc accaaagaac ttccaatgga atatgcagtc gaactcaatc gattgatcag
    36721 ttatgagatg gaaggcagtg ttgggtaaac ctgttatagg agctagaacc cttgatata
//
LOCUS       DAZKMK010000015        35513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000015 DAZKMK010000000
VERSION     DAZKMK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.015"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            220..537
                     /locus_tag="ACG8V1_000807"
     CDS             220..537
                     /locus_tag="ACG8V1_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328923.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            470..949
                     /locus_tag="ACG8V1_000808"
     CDS             470..949
                     /locus_tag="ACG8V1_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0328924.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            966..1142
                     /locus_tag="ACG8V1_000809"
     CDS             966..1142
                     /locus_tag="ACG8V1_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328925.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            1399..1830
                     /locus_tag="ACG8V1_000810"
     CDS             1399..1830
                     /locus_tag="ACG8V1_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328926.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            1836..2153
                     /locus_tag="ACG8V1_000811"
     CDS             1836..2153
                     /locus_tag="ACG8V1_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0328927.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            2272..2958
                     /locus_tag="ACG8V1_000812"
     CDS             2272..2958
                     /locus_tag="ACG8V1_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0328928.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            2933..4282
                     /locus_tag="ACG8V1_000813"
     CDS             2933..4282
                     /locus_tag="ACG8V1_000813"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0328929.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4273..4746
                     /locus_tag="ACG8V1_000814"
     CDS             4273..4746
                     /locus_tag="ACG8V1_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0328930.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            4748..5248
                     /locus_tag="ACG8V1_000815"
     CDS             4748..5248
                     /locus_tag="ACG8V1_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0328931.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            5263..7575
                     /locus_tag="ACG8V1_000816"
     CDS             5263..7575
                     /locus_tag="ACG8V1_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0328932.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            7822..8139
                     /locus_tag="ACG8V1_000817"
     CDS             7822..8139
                     /locus_tag="ACG8V1_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0328933.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            8136..8297
                     /locus_tag="ACG8V1_000818"
     CDS             8136..8297
                     /locus_tag="ACG8V1_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0328934.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            8300..8599
                     /locus_tag="ACG8V1_000819"
     CDS             8300..8599
                     /locus_tag="ACG8V1_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328935.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            8599..8913
                     /locus_tag="ACG8V1_000820"
     CDS             8599..8913
                     /locus_tag="ACG8V1_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0328936.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            8906..9436
                     /locus_tag="ACG8V1_000821"
     CDS             8906..9436
                     /locus_tag="ACG8V1_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0328937.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            9433..9702
                     /locus_tag="ACG8V1_000822"
     CDS             9433..9702
                     /locus_tag="ACG8V1_000822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328938.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            9632..9835
                     /locus_tag="ACG8V1_000823"
     CDS             9632..9835
                     /locus_tag="ACG8V1_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0328939.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            9832..10119
                     /locus_tag="ACG8V1_000824"
     CDS             9832..10119
                     /locus_tag="ACG8V1_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328940.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(10152..10403)
                     /locus_tag="ACG8V1_000825"
     CDS             complement(10152..10403)
                     /locus_tag="ACG8V1_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328941.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            10606..11019
                     /locus_tag="ACG8V1_000826"
     CDS             10606..11019
                     /locus_tag="ACG8V1_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0328942.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            11639..11803
                     /locus_tag="ACG8V1_000827"
     CDS             11639..11803
                     /locus_tag="ACG8V1_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328943.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            11800..12030
                     /locus_tag="ACG8V1_000828"
     CDS             11800..12030
                     /locus_tag="ACG8V1_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328944.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            12215..12760
                     /locus_tag="ACG8V1_000829"
     CDS             12215..12760
                     /locus_tag="ACG8V1_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0328945.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            12857..13078
                     /locus_tag="ACG8V1_000830"
     CDS             12857..13078
                     /locus_tag="ACG8V1_000830"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0328946.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(13235..13399)
                     /locus_tag="ACG8V1_000831"
     CDS             complement(13235..13399)
                     /locus_tag="ACG8V1_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0328947.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            13501..14022
                     /locus_tag="ACG8V1_000832"
     CDS             13501..14022
                     /locus_tag="ACG8V1_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0328948.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            14009..14449
                     /locus_tag="ACG8V1_000833"
                     /pseudo
     CDS             14009..14449
                     /locus_tag="ACG8V1_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(14489..14647)
                     /locus_tag="ACG8V1_000834"
     CDS             complement(14489..14647)
                     /locus_tag="ACG8V1_000834"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0328949.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            14740..15501
                     /locus_tag="ACG8V1_000835"
     CDS             14740..15501
                     /locus_tag="ACG8V1_000835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0328950.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            15688..17040
                     /locus_tag="ACG8V1_000836"
                     /pseudo
     CDS             15688..17040
                     /locus_tag="ACG8V1_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            17056..18276
                     /locus_tag="ACG8V1_000837"
     CDS             17056..18276
                     /locus_tag="ACG8V1_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0328951.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            18311..18988
                     /locus_tag="ACG8V1_000838"
     CDS             18311..18988
                     /locus_tag="ACG8V1_000838"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0328952.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            18985..20178
                     /locus_tag="ACG8V1_000839"
     CDS             18985..20178
                     /locus_tag="ACG8V1_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0328953.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            20193..20519
                     /locus_tag="ACG8V1_000840"
     CDS             20193..20519
                     /locus_tag="ACG8V1_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0328954.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            20523..20858
                     /locus_tag="ACG8V1_000841"
     CDS             20523..20858
                     /locus_tag="ACG8V1_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0328955.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            20855..21250
                     /locus_tag="ACG8V1_000842"
     CDS             20855..21250
                     /locus_tag="ACG8V1_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0328956.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            21247..21618
                     /locus_tag="ACG8V1_000843"
     CDS             21247..21618
                     /locus_tag="ACG8V1_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328957.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            21634..22194
                     /locus_tag="ACG8V1_000844"
     CDS             21634..22194
                     /locus_tag="ACG8V1_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0328958.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            22319..22630
                     /locus_tag="ACG8V1_000845"
     CDS             22319..22630
                     /locus_tag="ACG8V1_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328959.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            22654..22845
                     /locus_tag="ACG8V1_000846"
     CDS             22654..22845
                     /locus_tag="ACG8V1_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328960.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            22845..26693
                     /locus_tag="ACG8V1_000847"
     CDS             22845..26693
                     /locus_tag="ACG8V1_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0328961.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            26694..27566
                     /locus_tag="ACG8V1_000848"
     CDS             26694..27566
                     /locus_tag="ACG8V1_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0328962.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            27563..28612
                     /locus_tag="ACG8V1_000849"
     CDS             27563..28612
                     /locus_tag="ACG8V1_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328963.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            28616..29440
                     /locus_tag="ACG8V1_000850"
     CDS             28616..29440
                     /locus_tag="ACG8V1_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328964.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            29458..31518
                     /locus_tag="ACG8V1_000851"
     CDS             29458..31518
                     /locus_tag="ACG8V1_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0328965.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            31535..31684
                     /locus_tag="ACG8V1_000852"
     CDS             31535..31684
                     /locus_tag="ACG8V1_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328966.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            31681..32127
                     /locus_tag="ACG8V1_000853"
     CDS             31681..32127
                     /locus_tag="ACG8V1_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328967.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            32129..32266
                     /locus_tag="ACG8V1_000854"
     CDS             32129..32266
                     /locus_tag="ACG8V1_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0328968.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            32301..32543
                     /locus_tag="ACG8V1_000855"
     CDS             32301..32543
                     /locus_tag="ACG8V1_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0328969.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            32559..32756
                     /locus_tag="ACG8V1_000856"
     CDS             32559..32756
                     /locus_tag="ACG8V1_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0328970.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            32767..33783
                     /locus_tag="ACG8V1_000857"
     CDS             32767..33783
                     /locus_tag="ACG8V1_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0328971.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            33898..35106
                     /locus_tag="ACG8V1_000858"
     CDS             33898..35106
                     /locus_tag="ACG8V1_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328972.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            35090..35260
                     /locus_tag="ACG8V1_000859"
     CDS             35090..35260
                     /locus_tag="ACG8V1_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328973.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT        12516 a         5900 c         7802 g         9295 t
ORIGIN      
        1 tagaaatgat taaagtaata gcgacttggt aatcgctcaa aatttttatc aagtaattca
       61 ccaccttttt actcaaatta tatcaaaaag gagagaagaa ataatgcaag aattagtaat
      121 tttgaaaaat aaaaaaagac acgaacgccg gcaagcatga tcgtgtcagg gaaataaact
      181 atctttctat attttaccac agaaaggaat gaaccgtaaa tgctaattgc aacggataca
      241 ctagaccgca tctttttaaa agacgaatac aaactgcgca atatagatgc gtcaggaatt
      301 ttagtatttg atctttatga caatggaaaa attggtatct atcaagcaag tgatattgaa
      361 gaaacaaacc ttgctttcga acaaattgat gattctgtgg aattggattt agatgaggca
      421 atcctagctt ttgaacaaat tgcaaaatta ttaaaggagg cacaaaagaa tggcaactct
      481 ttaccaactc agcgagtcat atatcaaagt cctggaactg gcagaacaat tggatgaaga
      541 aattcttcgc gatactctcg attcgattaa tgaagcgatc gagtataagg cagaaaactt
      601 agcaaaaata gttaaagaag tagaagggaa agctgagtta atcgatagtg aaattaaacg
      661 tctgcaggaa cgtaagacat cgcttttgaa caatgctaag agtatcaagc actatttaca
      721 agaggaaatg gaaaagactg gtaaaacgaa gattaaaggt gaattattca acattggaat
      781 tcaaaataat ccagtatcgg tcaatgtaat caatgagaac ttaattccaa aaggattctt
      841 tacccctgtg cctcccaaat tggataaaaa gcaattgaag gaggaactga agcacggaga
      901 tattcctggt gctgaactcg tccaaacaaa aggtttgagg ataagatgat gaaggaggag
      961 aaataatgga acacgaaaac gtaaaagacg ctttgaaagc tgctatcgaa atcgcggaag
     1021 ctaaaggaat aaaggttgac ggcaaaccag caactgtcca tgacattcaa aatttgacga
     1081 aagaacatct ttattttatc gccgacttgc ttggtttgtc ggaattgtat ttagacaaat
     1141 aaccccacac ggaaggagtg gctagggtgg ttgtattaca agtaatagtc tgcgtaacat
     1201 tcatttggtc gtgcataccg gtttgtatat atatcatgaa gaagcttcgc cctgagtctt
     1261 acaaattata cgcaatttac agcataacag tagctcttgc atttacattg tttgcaattt
     1321 ctaggcttcc gttttaatcg tgggaatgaa tttttaatga actaaattat ctataaagca
     1381 aaaaaaggag aaacaacatt gaaaaacaaa ctagcaaaaa caacagcaat catcggacta
     1441 gcactaggta gcggcgttat cggctacgca gcaagcaatg cgtttcaaga tttggacacg
     1501 atcaaagcaa atttcaacac agtcctacaa tacggtcaaa cgaaatcgca acgtgtgtca
     1561 gaacttgaat cacagctatc caacaacact cgcacacagg agcagctgaa agccgaaatt
     1621 gagcaaatca aatcggacaa gcagaaggaa attgaagcca agcaacgaga agtcgaacaa
     1681 aagcaacagg agatcgctac aaagcaacag gaagccgata gcttgcgcca acaactatcc
     1741 acagtgcaaa acgacaagga gcagttagaa cagcgcgtga gcgagttacg gcagtatacg
     1801 gatcaaaagg taggggagtt ggggaaatga aagagttgac tatttatgac ccaaatattt
     1861 catgggctaa acacacaatt aaagtttctt ttatgatttg gggttacaaa ggatatgtta
     1921 cttacaaagt gggcggtaac actaagggtt tatcattaat agctattgat tcagatgatc
     1981 tatatgatgc taatttcgag gataacccag ttaatttccg tgaccttgat gaagattggt
     2041 tctctatgga acttactaac gataaaggcg acagtacact tgtagaagat gaattcgaca
     2101 gactaggtga ttatattgtc ggtgttgaaa ttattgcgca cgaaccagag taggagagtg
     2161 aatacaaaat gattaaaaga atcactatat cagttgaaga agatagtgga tcaacaatgc
     2221 agcataccga caatcgaaaa acttttttaa ccaatagaaa ggagaatttt gatgcagatt
     2281 aagaaggcga cggatatcga acaaaccaag ggaacatatt tgatctacgg tgctccagga
     2341 aaaggtaaaa cttcaacggc taaatatttt ccagggaaaa cactgatcct tgatattgat
     2401 cggacttcaa aagtccttag aggtgagaaa aatgtcgata tcgtatatat cgacaacgaa
     2461 gatacctgga acgactgggg caacacgttg gctgatttaa ctaccaatta tgtaggtgtt
     2521 tatgacaacg tggttgtcga taatgtcagc gaattggaac ggtgtattct gtccagttta
     2581 ggcgcagaag gaaaaaacaa cggcgttcct tcgcaaggcg attatcaata tatgcaattt
     2641 agaatggtga acagcctacg ctatatgaaa aatttagaca gcaaccttat ctggaccgca
     2701 tgggaagaaa tcgatttgtg gacggattcg gatggatctt cttacaacat cgctttgccg
     2761 cagatcaatc gaaagattcg aaacaatatt ttgggattat gtgacgttgt tggtcgattg
     2821 atggtaaagg aagacgatga acgcggattt attttacaag ccacagattc aacctatgcc
     2881 aaaaatcaat tggataatag atctggctgt aagcaaagtg agctgatttt agatggaatt
     2941 gtacgactac cagaatgatt tagtcgatcg tgcaagacaa gcctatgttg atggttacaa
     3001 ggcaccatgt atcgtatcac cgtgcggttc tggaaaatca gtcatggttg cagagatcgc
     3061 taaacgaacc acacgaaaag gcaacagagt gttgttcttg gtccatcgtc gtgaattgat
     3121 tgatcagatc aaagatacgt ttcaaaagat gggcgtagat aatcagcttg taaatttcgg
     3181 catggttcaa acagtcgtac gacatcttaa aaccatcaag aaaccacaac tgatcatcac
     3241 tgacgaaaac catcacgggt tagcagcgtc ctacagaaaa atctatgagt attattcgga
     3301 cgttccgcga ttaggtttta ccgcgacacc catccggtta aacggtagtg ggttggggga
     3361 tgtgaacgac ttgctaattg aaggagtatc ggctaaatgg ttgattgaga atcatagatt
     3421 agctccttat gaatattatg cacctaaatt gatcgataca gccgaattaa agaaagcttc
     3481 gactggagat tttacaaaga aatcaatgga taaggcagtg aagaatacta tttatggcga
     3541 cgtgttgaaa cattataaaa cgttagcaga aggtgaacag gcaatagcat actgtcacag
     3601 tatcgatgca agtaaacaca ctgctgaaat attcaacgat gctggttata aagcaacaca
     3661 tatcgatgca aaaactccaa aagatgaacg tgcagatatt atcgaagcgt tcagaaatca
     3721 tgaaattaaa atcctatgca atgttgattt gatcggagaa gggtttgatg ttcctgattg
     3781 ttcgactgta attatgttga gacctactca atcgttatca ttgtacattc agcaatcgat
     3841 gagggggatg cgttaccgac cagataaaac gtcaatcatc attgaccatg tgggtaatgt
     3901 gaaccgtttt ggattgcctg atatggatcg tgaatggagt ttagatacaa aaaagaaaac
     3961 aaaaactgat agtgatttat ctatcgttca atgcgcgttt tgttttggtg cttacgaacg
     4021 gccaaaaggc gataatattt gtccgtattg cggagagttg cagccgattg aagaacgaaa
     4081 gtcagagtta gaaatcgatg aatcggctga attaatgaaa gtcggagaaa cgaaaattac
     4141 tttgaatttt gagaacaata aatattacaa catgacggaa gatgaagcag aaagtataga
     4201 ggacttatat gccatagcaa gagctaaagg atttaaacct ggttgggctt atatggctgc
     4261 taagaggaag gggtggttgt aggaatgggc ggtatttttg gtaaaaaagc agaagatgtt
     4321 caaggttcaa taataaatgg cgtaacaata ataggtgata ctggaaaaag agatagttca
     4381 ggcaatcaaa ttgtaatcgc tagaaataaa aataacgaag tttttgaatc ttctctatct
     4441 aatataaaaa gaggtgttgt taacgggaca ggcgaagaga gtggctacgt taatttaaaa
     4501 aaagctaatg aaaaatacca tgttgaagga acttacgtta aggctttgtc tgtcggtgga
     4561 acaaaaggcg ttaattggga taaacgaaga aataaatgga aagcacgatt atatttcaaa
     4621 ggagaatatg tcttacaaaa ggatttcaac accaaagaag aagcaatcca agcacgactt
     4681 gcagcagaag aaaaatattt caaacctatc ttagaaaaat atgaaaataa aaaggagaat
     4741 gattaacatg acaggattta acttagactt ttcaaaagcg caacaaggga acgaaatcaa
     4801 agacggtacg tacgaagtgg tcgtaaacaa agctgtagaa aatgcaacta aatcaggcgc
     4861 agaatttatc gatatcgatt tgatcgttcg taacgatgtg gatcaaccgt tccaaaataa
     4921 acacattttc gcaaaaatct ggaaagccaa agctaccggc aaatataacg aaggtatgat
     4981 catggcaatt gcacaagcgc tacagttgga agacggaaaa tcatataacg ggtttgatga
     5041 attgctggcg gactttgttt tgaaaaccgc ctcagtacgc gtgaaaactg aagagtcaaa
     5101 tggatataaa aacgtcaatg tgaagtcttg ggataaaacg aacacacggg gcgtaatgaa
     5161 tcaccaattc aaaaatggtg atgaaccatc cttcggaccg gaacgttcaa gagcgacaac
     5221 ggttagaaat gacaatcttc cattttaatt gaggtgtaat caatgtatga aaaaattcca
     5281 aaagagctta aaaatctcaa acaatggtgt gtttataagc tagtttggga tgaaaagcgg
     5341 cagaagcaca cgaagatccc ttataacgcc aacaacgggc ataaagccaa atccaatgac
     5401 gagagtacct ggtctgattt tcagaccgct ctcgctgcta ttgatcacta cggaatgagt
     5461 ggactggggt tctttttcaa accaccttat ttcggcattg atatcgataa tgctgaaggt
     5521 gaagttgagc gatttaaatc aggtgatata gaagaaaata ttatctatga atttatcgaa
     5581 agcatgagat cctatgctga gtattctcaa tcaggtactg gcatccacat tattgctcgc
     5641 ggagaattgc ccggcggaag acgcaggaaa ggtgacgtag aaatgtacca gaacggccga
     5701 ttcttcgtta tgactggaaa tgccgcatca aaatatttag aaataactga gccgaatcca
     5761 aaggatatca aacgcctata tgatcgctac gttggagatg aaaaaatcat ccaattcaaa
     5821 gaagaaaatc cgctgatgaa taccgttgat ttaccggttg aagaaattat caaacgtgct
     5881 gaatcgtctt cccaaggggc aagattcaaa gtctttatga acggcggttg ggaagctttt
     5941 tattcctcgc aatccgaagc ggatatggcg ttcgccaacg acttggcttt ttggaccggt
     6001 agagactttg aaaaaatgga tgagattttc agagcgtctg caatgatccg tagcaaatat
     6061 gaccaaaaaa ggggatcggc aacttacgga gaatctctac taaataaggc aattgctgat
     6121 acaaattccg tctacaaccc caaacggaaa tctgatttta agatttttat caaagaccag
     6181 gaacagccaa aagaggaaaa atactattcg tacgacgaca ccggcaacgc ggatcggttt
     6241 actgatatct acggaacgtt agtcaaatat tcgtacattg ataaatcttg gtattactac
     6301 gatgggaaag tttggctaca ggacaatacc ggcgaagttc ggaaaatgat cgatacgaca
     6361 gtagacatca tgggaaaaga accgttaacg atccctgaag gcgcagacga tgaaaccaaa
     6421 gaagctttga tgaaagcgaa agaaaaacac gtcaagcgtt ctcgcagcaa tgccggaaaa
     6481 aacgctatga tggacgaatt gaaacatcga ctatccgtat tgccggaaga gttcgacaaa
     6541 gataaaacgc tgttcaacac acaaagcggc tatctgtctt tgcatgacgg tcttctacac
     6601 gagcatgaga tagacaaaat gtttacacgt gtttcaaatg tcgagtacac agaatcggtc
     6661 gattgtccta tgtgggaaga atttattaat cagatttttg acaatgatca agaattaatc
     6721 agatatatcc agaaatgtgt cggatattcc ctgaccggat ctaccagaga gcaatgtatg
     6781 ttcatcctct acggtcatgg gtcaaatgga aagtcggtat ttttggaaat tatttctgaa
     6841 ttgatgggaa actatgcgat gactatgcag gcacaaacga tcatggtcaa gcaaagccaa
     6901 tcatcagcaa acagtgatat tgcacgattg aaaggtgcgc ggttggtcac ttcgtcagaa
     6961 ccgaatgaag gcgtccgact ggacgaaggt ctggtcaaac aattgactgg tggtgataaa
     7021 gtcacggctc ggcaccttta tggaaaagaa ttcgagttcg aacctgaatt caagttgtgg
     7081 ctggcaacga accacaagcc catcatccgt gggaccgatg acggcatctg gcgacggtta
     7141 aatttaattc cattcactgt ccaaatcccg gatcacaaaa aggataaaaa tctgaaattt
     7201 aaattgcaga cggagttgca agggatcctt aaatgggcaa tcgatggttg cctgttgtgg
     7261 cagagagagg gcttggagaa accaaagtcg gttgttgcag cgagtcagga ttatcgaaac
     7321 gaaatggatc aaataggtac atttatcgaa acgtgttgcg aaacaggtcc aggactaaaa
     7381 atatcaggcg gagaattata caaagtttat cgtgaatggg catcagataa tggcgagcat
     7441 actttcacta acacgaagtt tggcagagag atttcaaaga aatatagcaa agaaaaagct
     7501 ggtggattta tggtttataa aggaatcaca ttaaaaccaa gaaaatacga taacgttaga
     7561 gaacttttta aataaatctg gagggttctg gagggttgag aacgaaaccc tccaaccctt
     7621 agaaacgttg atatatcagt atttatatta tatttttctt tctttctgga gggttttaaa
     7681 aaaataataa agtattaaag taaaaaataa aaagtaaatt atataaaaaa gaataggttt
     7741 ttgggtctga ccctccaaac tctccaccaa aatcttccta aatgctttga taccaacgat
     7801 taaacagtct ggagggttac attgaaatca gaacatcaaa tacaagacga gatcagaatt
     7861 gccttatcaa aacatggttg cacgattttt cgatccaatg cgggacaagt gacaacgatc
     7921 gaagggcggc aattttacgg gatgccaaaa gggttcccag atctatgtgg acatcgtaat
     7981 tctgatggga aagcaattta tatagaagta aaaaatgaac gcggtaagtt gcgtgaagat
     8041 caaaaaaggt ttgctgaatt tttaaacggt caacctgtac tatacggtgt tgcacgatca
     8101 gtggaagaag cattgaaaat tgtggaggaa tcatcatgac aacagaagaa gtgattcaaa
     8161 tgcgtattcg aagccttcag cgtgagattg acgatctgga acggacaaag gcagtgatgg
     8221 tcaatgaaac ggcgaggaag gcaatcgatt tgcacataga gaatttaaga agggaaatcc
     8281 atcgattgga ggaatgagcg tggataagaa agcagcaatg aaacgaatca tcgaactgac
     8341 acattctgag aattggcaag aagacaaaga aatagttgca gaagtccaaa agctcggtaa
     8401 atcaatgtgg agtgaaaaaa ccaaacggag aacaccgaga aaaattgcaa tctggcatgg
     8461 tgaccgaatt ctagtaacag gtactgctga acagttatct gaaattactg gattaagcaa
     8521 aaacattatc tgggatagag ctaaacgcga gaacgttgat tcgaaaggtc gtcaatttaa
     8581 acattgggag aagaaataat ggaggaacta atcacaaaag tagagcagtg ggcaaaagat
     8641 aagggattgg atcaagctga ttccagcaag caaatgttaa aaacgatcga agagattggg
     8701 gaagttgccg cttctctagc tagaaaagat gaatatggtt taagagacgg aattggagac
     8761 gtagtagtaa ccttgattat tttagctatg caaaatgata tggatttgta cgagtgtctg
     8821 aaccaagcat acaacgaaat caaaggacgc acaggaaaaa tggtagatgg tgtattcgtg
     8881 aagtcgagtg atttggagga cagcgatgaa taaaaaagta ttaattgata aacaagcatt
     8941 gattgatgaa ttaatgaaaa tacctggtgt gggatctaat agtgacgctt tagaaacgat
     9001 taaacgtttc ccatcgtatg aaccgcagaa gccagttgtg cctaagtttg tggcggaact
     9061 actcgactat tatcgtcagt caacagatgt tgatttatta gctttattga taactttcca
     9121 tgattggtat tatcgcaaaa ctaaagacgg tgagcatgaa gaagcaattg attggctagt
     9181 tgatcatcct gaaatttata tgcgtgcatg gctgtttggc tacgagctcg agaaagagcc
     9241 gttgtattgc gtaataatca atggtagata tcttgtcaaa gtattcagca atacaaacgt
     9301 tgtcatatta gttccagcag atgaattttc acagtacgtt actcaaaaat ttcaattaac
     9361 tgaaaaacaa atcaaagcaa ttgatgaaag atactggccg tttgctgtgc cagtggatga
     9421 ggtggtagaa ggatgagcga cccatacatt actaaattag aaaagttgtc cgaagatttt
     9481 gagaagaata aaaataaact aactaaagat aaacttatag aactggttag gttggttatc
     9541 agaggagcta ttacaagaga aaaggcgcag caaaacatag aaatttttgc tcgtagtgtc
     9601 cgagatggat tagagaagaa agttaaacaa aatgaacaac agacaccgca gaataacaaa
     9661 actaagaaaa cgggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc
     9721 agcagaagaa acatataaag tggcaagtca gtgtgttgct gatgcgagcg gcgctattcg
     9781 taagtttgga atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta
     9841 aaagacggat tttactccag cagtcatggt atcggcggtt taatgctaga tatgccgaca
     9901 aagaacccta aaacacgtga gaaaccaaaa ttcaaagtcg gtgacatggt tcgctgtgaa
     9961 gcagaaggat tcatctatcc attttgtgga tatgtagaac atctctataa tcactcagca
    10021 atcattcgta ttgaaaacac gatggaatgt gacaagtggt tagcgaaaag caaagagaat
    10081 ttagcagtag cgagattggt ggatatggag gttatataaa cagcgaaaaa atgtacttct
    10141 aagagaaaaa tttatttttt ataaaaatag gaaagtatca ttatgaaaaa gaccacagag
    10201 ataaataaac ctaccacata aaaaatgaat atttcttgcg attcaatata ggtatattta
    10261 acaatttgta caataaaaaa tactataggt aaaaaagata acatgaggag cttatttgtt
    10321 ctagagtgtt tgacaaagta acaaaatgcg acgataccaa tacaaacggg aatccaaaat
    10381 aacagcatgc tccacatagt cactcaacca tctcctatac gctttattat caatagataa
    10441 tatcattttt cgtaataaag ataaaaaaat aagtaaggct tctcattaaa atataaaaaa
    10501 gacagccgac cactgactgc ctatataaga gtattgaaat aaaaagctgc tgatataata
    10561 aattccacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
    10621 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
    10681 ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat tacggatatg
    10741 ccgaaggcgc cgaagcacgg caataaggca gaagacgcaa tcattcagat gatggatata
    10801 gaagcggaga gagacgcgat tctagcagcc ttgatggctc ttagtctgat tagtcgtcag
    10861 atactctact atagcttttg tgtgccagat agcttctcaa actacagaat tagccgtgaa
    10921 gtgggttatt cagaaagaag tatacaacgg atgaagtcgg aagctctaat agagtttgca
    10981 gaagcatata aacacggaag aataattgct tataaataat ttggcggctt tttggcggaa
    11041 tgatggcggt ttttagctat ttaccagtga tattatggta gtgtcgaaag attagtgata
    11101 ggtctaagac aaaataataa taaaaggaac atcgttttat tattgtttca caattaagct
    11161 tcgatagaca gcaacggaaa tattaagaat aaggatgtga attttaactc cttctaaatt
    11221 gttcttatta tctatcatcc gttgctgtct attgtttttt taattattca cacgataact
    11281 aaaggtgagc gaagagaaat gattccatta ataatttcaa tttttgcgct ttgtcttaat
    11341 gtctatatga ttggatttaa aaatgggcaa aataaaagat agtagtaact aagaaagaaa
    11401 tttttatatt gtcactgtgg cggaaagggt agacgctaag catgtgtgct aggtcaatgc
    11461 ttcggcaact atgcaatgtt cgattcattg ccagtgactt tggcaaccga ggcatcggcg
    11521 gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg tgcatcgtgt aggttgctat
    11581 tacatattag atcactcgtt gagtggtctt tttattttac ataaaggagg taacaacgat
    11641 gtatagacca caatacttag aacagaagta tgaagtaatc actgtgcaaa acggtaacgg
    11701 tgagatagta cgaaagtata gaagaccaat aaagagcgat acatataaac gaaaggaaaa
    11761 caatgaagtt atttcttttc gcagaaggag aaaagccaaa tgagaaacta ctggtatgta
    11821 tcgctaacta atgaatatcc tcgaaccatt gatgattgtt cagtgcgtgt tgtgcgttct
    11881 gtacaaatca aagggaagta ctctattgtc gaaatgctaa gagaagctac accaaacgaa
    11941 gtggataaat gcaagctgat atattgcggt catggctatt ggaaagacga gtatatccaa
    12001 caaaacattg agaggtggat agatagatga gttacctcga acatttgaaa cgttgctaca
    12061 tgcattctaa gaataaactt cctgacagct acactgtaga tgatgttgct atacatgtat
    12121 tgaagactga gagccatagc agtcctgatg gtagcagtaa ggagcagacg ctcgcgtggt
    12181 tcaagttctt taagtggatt aaggaggaag agtaatgcac atgcacatag gagaaaagat
    12241 atataaattg agacggctta ataacgaaac aatgaacgac ttagttaaac aaactggagt
    12301 gtcaaaatca tggttatctg atattgagtc tggaaaaaag caacgcgttg attttaataa
    12361 gatatgtgca atagccaaac attataaagt tagcttagaa tactttcggg atgattattt
    12421 ggatagtgat gaacacgagg atattattcc tttgacaggc gaaacaagag ttaaatcagt
    12481 gaaatcgctt gaacaaagcc taaagtatct gaataacaaa caagaaccta accttctaga
    12541 gatcaaacta aaagatactg actcagtacc agaggtttgg tacaagggtg agaggttgga
    12601 tgaattgcct aaaggattag tagatgtctc gtatcattgg aagactgatg attttactaa
    12661 tgatgataga ggagcgaacg acatcacgat tcaatacttt tctagcttta atgataagta
    12721 tccagataag aaaacaatcg gacacaagag agatatgtaa atgaaagaag ttagacctag
    12781 agacgaaata gacaaactat acaagaccaa acgatggcga gacctaaggc aagtagtaat
    12841 agctagggac ttcggcatgt gccaagagtg caagcgtcga gggcggaaca caaggggcac
    12901 gatcatccat cacatagtcg aggcgaggga agacctgtca ctgttctggt ccgtagataa
    12961 ccttgaatgt atctgtgtag cttgtcacaa cagagagcat ccagagagat caggcgggaa
    13021 gaagaaacca aaacctaaat cacatatcgt taaaatgtat tcaactcctg aaagataagt
    13081 ttgcagcgaa atgaaggtag cccccctact ctaaaagatt aaagagtaag gcttgagaag
    13141 aacggtgctg tccttccttc gtaaaaagac cgcttttcaa gttttttgga gaaaaaggaa
    13201 aaagccgacc aatttaagcc ggctttggac gaagctattt cttagtccat ttgtttcctt
    13261 tttgagaagt agggggtaat cggtcgcctg gatcaatttt tacttcttgt ccgccttgga
    13321 catttccacc acgaggaccc acttctttgt aggttccttt tggtttgttg tcttcgccgg
    13381 gtttatagag ttctcccata ggaatccctc cttaacaaat ttcggcacag cactgccgat
    13441 aacctaatta taaggattgt gataacgatt tcaatctatc ttttgaaaga aggtgatatt
    13501 atgccgcaac cagcgaagag tgcaaaatta caattattaa acggaaaccc aaataagaag
    13561 aataccgaag aactccgcaa gcgagcggcc gcagaagaca aattaaaaat ggctactgac
    13621 aaaatcaaac cgccgttatg gctagattcg ctaggaaagg atacctttga gtttatcgcc
    13681 gatgaattgc tgtctgtgga tttaatcagt aatccggacg tccatacaat ggctctatac
    13741 tccaattggt attcgcaata cgtttcttta gaaaaacagc ttcgaaaact acaacgagag
    13801 tacaagttga actatgcgct tgcgaaaaag gaggcagagg cgagaggtga gccgtttaat
    13861 gaacctaatg aattaattgg taatccgctc tctcggcaga tggatacagc gtctcggaat
    13921 ctccgttctt ttggcgctga tttaggacta tcaccagcag ccagagctaa gttagctatt
    13981 aagatggctg atgatggtgg tgatgacgat gacgacttct aatattttgg atatgtctta
    14041 cacagaacgt gtggactatt ggcaaagcta tcttgaggag caagcttctt ggggtggctt
    14101 tttaaaatgt ccatatccgg aattgttaac tacttggtat gcggaacgat taatcgatgg
    14161 aagcatacca gccagcaaag aaaatattca agctgctaaa cggcatatgc gtgatttgca
    14221 gcgccaagga acagatgatt ttccttggat ctttgacgaa gaaaaaggtc accggcctat
    14281 tagatatatc gaaaaaaaat gtaaaccaac tgaaggcgac tttggttcgt ttgttttgca
    14341 accttggcag catttcataa ttggatccat gtacggatgg gtacatcgtg atacaggaga
    14401 gcgtcgcttc cgcgaggctc ttatttttgt tggacgtaaa aacggtaagt tttgccgttt
    14461 aaaaatcggg caaaatcggt gaaaaccttt atttttggct ttcttttagt aataattcga
    14521 tagctttatc aagcaattta gacataggaa ccatagtttc ttccgacatt ttttttaatc
    14581 cttcgtataa atctttatca attgcgttag aaattctaat tctgtttttt agtccgtatt
    14641 tgtccatgtg tgacactcct ttttatttta ctataacatc cgattccacc gcttgcaact
    14701 accatcaatt gatggtagta tataaaagag gtgagtagat tgaaaaaata cattatttac
    14761 aaacacttaa ataaaataaa tggcaaaatt tatatcggtg tcacaaatga tattggcaga
    14821 aggtggagga gcggtgggat tgaatataaa ccaccaaaga acgaaaccca acaccacaga
    14881 agtttttgga atgcaataca aaaatacggg tgggaaaatt ttgatcattt gattattgaa
    14941 gaagacttaa cgatgaaaga agcttttgag aaagaaaagt tttatattga actttatgac
    15001 tcaactaata agaaaaaagg ttataacatc gcaaaaggcg gaaatggcgg aataatatat
    15061 aaagttcacc ccaaaggaat gctagggaaa aaacaatcta aagaattttc ttcaaatcac
    15121 agtaaatggg caaagaacca taagaataat tgtatgacaa acggtgatgt tgtatggggt
    15181 gttactcacg aacacccaaa aggcatgttg ggaaagcacc acagcaaaga atcaattatg
    15241 aaaaagaaag catatagtgg tgaaaatgct gtaacctcaa aaccaattgt cgcaattgaa
    15301 cgggatggaa ctaaaagaga atttcacagc gctaagcttt gcatggctta ctattcgatt
    15361 agtacatgtg ttttttaccg acttttaaaa gatggcgccc cttatgtgat aaatcctaaa
    15421 gcgaactaca gaaataaaga aaaaatatta gctatcgaag gaattatgtt caaacataaa
    15481 gaagataccg aggtaagtta gtagattgcg aaaggttact aactaccgta acgcgtagga
    15541 gttgaataaa tataatgctc ccaagagtgt ccgacagcag ataatttgct gaaaatgtac
    15601 gctaaactgg gccagaactg actgaccgat gaaaatgagg gtgacctcca gagtgcgaga
    15661 taaaaagctc gcagataata acaatcgaaa acatcactaa tttcaggcct ttccacatac
    15721 atggtcgctt atgatgatga acaaggcgcc aacgtttacg tattggcaaa tgctcgtgat
    15781 caagcaagct tgttgtttga taaggccgca gaaatggtca aacaatcgcc ggcgctcttt
    15841 aagaaatttg gtaagcctaa acgatcaagt attaattatg ctcccgcctt ttctaaaatg
    15901 gaaccacgcg cctcagatag ccggaaattg gatgggctaa acactcactt tggtattttt
    15961 gacgagatcc acgagtttac gaattacaat ctgatcaacg ttatcaagaa atcaagagga
    16021 accagaaaac agcctctgat agtttatatc acaactgctg gatatgtatt agatggtccg
    16081 ttgatgtctt attttgagca aggtgtggac tgtttggaac atttggaaga tgacatcgat
    16141 gaacggactt tctattatct ggcaaaactt gacagtgcgg aagaggctga tgacccaaga
    16201 ttatggatca aagccaatcc gaatatttgt ctaatgaatt ttgttggcat gctagatgac
    16261 tatgttaagg ataaaaaaga tccaaaagaa tatgctgact ggattaccaa gcaatttaac
    16321 ttgttttccg atatcgatga gctgtcattt gtcgatatgc ctaccattaa acgaaacaat
    16381 aaaaccatcg atattgaaac gctcaaaggt aagaagtgtg tcggtggttt tgacttgtcc
    16441 gaaacggaag actttaccgc agccgtttta gaatttccgc ttgaaacagg cgaggtattc
    16501 attttgcaac acacatggat cccacaagct agatttgatc gagataacaa tcaagagcgt
    16561 atcaaagcgt gggagaaggt gggagatcta acgattattc ctggtgatta cgtcaattat
    16621 gaatacgtct taaattggtt tgtagaaaat tcgaaaatct atgacattgt aaaaatcaat
    16681 tatgacaagg ccaaggcgct acgattaaat aaagaactag aaaatgcagg atttgaaacc
    16741 aacgagattc ggcaagggtt tctatcatta ggtgggccaa tgcaaaactt caaggaaatg
    16801 ctattggacg gtaaggtgat tttcaacaat tccaagcttt accgatggta tctatccaac
    16861 gtcaagctgg tgatggatcg caactcaaac tggatgccgt ctaagcagtc caagagtaga
    16921 aaaatagatg gttttgcagc aagtttgaac agccacgccg aagtgttgaa tatgttggtt
    16981 aatcctgtcg gaaccgggaa agtaacctat tactcgattt ccgatttaat gaatatgtaa
    17041 gaaaggtgtg gaggaatgag tattttagat cgtttgcgtt cttttggccg agcgaagccg
    17101 aaagcgagca aacaagagta ttttttgaat gacccgggat tgataccgta tttagtcgga
    17161 aaagatgaaa tatcagaagg gattttttcc gtaattagcc gtgtatcgaa cgtttttgcg
    17221 tctctccctc tcaaaatgat agatgtggag tttggccaac cggacgactg tcctgcatac
    17281 aacttgttga gcgaaggccc tcgatatttt acaaagtttg attttttccg ggacgtggaa
    17341 gttttgagaa actaccaagg gaatgcgtat gtgcagattt tccgaaatat caatggagaa
    17401 gtagcagata tggcgttagt aaaacctggt gcttgccatc cagtgattga tatggatagc
    17461 ggggagcttt actaccaagt aactgcgact gacaaaggca gttacaagca agttatctat
    17521 gtacattaca tggaaatgct tcactttaaa caaccgaggt ttggcggctt ggaaggtaca
    17581 gaccccacaa aagtattaac gaataccctc ggatatgatc gagaagtccg aaaaatctct
    17641 ttaagtcagc ttaaaggaag taatgaaggg ctaaaagtta agtttgctag caatatggat
    17701 gaagaagcta aaaaagctac agttaaaaac attgctgatt tttatcgaca aaacggtgga
    17761 ctacttgtgg aagaaaacgg tgtagaaatc gaacgtttac aacgagagct ggtagacagc
    17821 aagcttttag atactgataa agtatctcgc tccagaatcg ctatggtcta caatgtgccg
    17881 gagcatttca tcgggaataa ccagtcgagt tactcctctc aggaacagct caatatggaa
    17941 tttttgacgt acaatctagt accgaccgtt aatcaatatg aagcggaact aaataagaaa
    18001 acactatcga gaaccgaaaa agcaaaaggt tatcgatata agtttaatgt cgcaagtttg
    18061 ctaagagctg acactcaggc aaggggacaa ttctatcaaa tcatgcgacg aggcggagca
    18121 tactcagcga atgatgtccg aagatttgag gacttgcaac cgataaataa aaccggtatg
    18181 gatgattacc atatttccgg agacctatat ccaatcgata tggatccaac attaagaaaa
    18241 acaacctcgt ctaaaagcgt agccgaaaac ggttaggttt ttttagtttg caccgaaggg
    18301 aggtggaaaa atgaaaaaag tgacgttaag cggcgatgtc gtggataacg ataccgcgtg
    18361 gctttatgac tggtttggga tcaattgtat ctcaccaggg aaaatttctg ccgctcttac
    18421 agaagcagcg ggggatgaag tagaacttga tatctcatcg aacggtgggg atgtcctagc
    18481 ggcaagcgaa atatataccg ctatccgtgc ctatccggga aaggtatccg gaaatgttgt
    18541 gagtattgca gcaagcgctg caagtgtaat cgcttgcgct tgcgaaccgc ttagaatctc
    18601 acctacggca cacatcatga ttcataacgc atgggtgacc actagtggca acgctgagga
    18661 attaaaagcc aatgcagaaa tgttaagcag tgtggatgag tctattgtta atgcttacga
    18721 gatcaaaaca ggactcgatc ggaaaaaact tgctgattta atggcgaaag atacttggtt
    18781 aaatgctcaa acagcagtag cagaagggtt tgcagatgaa attatgtttg cagaagcacc
    18841 agtaacggta ctcaatgcct ctcaaccggt tattccaaaa aacgcagtaa ctaagttgaa
    18901 aaatttaata ctcaaagcgg aagcaccgca agaagaaaca ctcttacaga aaaaactaaa
    18961 agccttaaat ggagggaaaa acgaatgaat ttagaacaat taaaaaatgc gtgggtcgag
    19021 gcgggaagta aagtctctga cttaaatgca caactcaatg cagcattggt tgacgatgaa
    19081 aaaacagaag aagatgtagt aagtttgcaa gcacaagtaa aagcagcacg ggctaaacgg
    19141 gacggattga aagagcaagt ggcaaatatg gaagccgaac aagtcttaaa cgtcaaaaaa
    19201 gaaccattag ataaaaaaga tgaaaacttg aaaaacaagt ttatcaaaga ctttaaagcg
    19261 atggtcaatg gtgatcctgc tattatggct actttgacat ctaatacgga tgaatctggt
    19321 aatgctatcg gattgactat tcctgtagat gtgcaaacga ctattcatac tttggttcgt
    19381 cggtttgact ctttgcaaga atacgtaaac gttgaaaaag tgaccactac cagcggttct
    19441 cgcgtttatg aaaaatggtc tgatattaca ccgttgactg ctttggatac tgaagacggt
    19501 gaaatcccag caaatgatga tcctgcactt tacttgatca aatacttgat caaacgctat
    19561 gcaggtattt ccacagtaac taacagcttg ctaaaagata ctgccgaaaa cattttggca
    19621 tggttgtcta aatggatcgc gaaaaaagta gttgttactc gcaatacaaa aatcttggca
    19681 gctattgatg gaatcaaagc ggcacaaaag aaagatgtta cagatgttga tggaattaaa
    19741 gatatcgtaa acgtccaact tgatccagct atcgaagcta catctatgtt tattacaaac
    19801 caagatggct tcaatgtttt agataaagtg aaacgtgctg atggatctta cttgttacaa
    19861 aaagacgtag cttctgcaac tggatatacc ttcttaggta aaccaatcaa gaaaattgct
    19921 tctcgtttct tgccaaacaa aggtacgcaa gctactccta aatatccact gtacattggt
    19981 gatctgaaag aagccgttac attgtatgat cgcgaaaaca tgagcttgct gacaacgaat
    20041 attggtggtg gagcttttga aacagacacc actaaagtac gcgtcatcga tcgcttcgat
    20101 gtgcaactag ttgatgatga agcggttgtt ttggctactt ttacaactat tgcgaacgag
    20161 acaccggcga aagtttaagg agctgattcc ttatgattct tgatcctaaa atggatttag
    20221 gcgaaatcaa aaacgcacta aagattgata ccgatgatga cgatgtggaa gtaagccgtg
    20281 cggcacaagc tgcaattgca tacattagag gggctatcgg gaatgataag ccctcttttt
    20341 ataagcaaga aaatgaaacg gttgatctga ttaatttagc tatcctgcaa ttagcagatc
    20401 attattacaa cgctcgatca gcaaccgtga gtgggaactt gcgagagtac gatttaggtt
    20461 ttacaagcct aatcttgcaa ctcaaagcaa gttatttgct ttttgtggag gaggagtagc
    20521 gtatgcccct tatccaaaca ggaaatttaa atcaacgcat caagtttgtc cgagatacga
    20581 cggttaagga tgaggacggg caagttgtcc cgacttctac aaccattctt acttgctggg
    20641 caagtgtgca gacacaacgc ctgaacgata ttaagacgtc gattggtacg gctttggaag
    20701 gaacactgac gttcattatc cgctaccaac aaaaatcaga gctgaccaat gatatgaaag
    20761 tgcgttggaa tggaaaaacg tttgaaatca ttacgattac gaaaggcgag tttgcgaagg
    20821 acttcacgac aatcattgca aaagaggttt caaaatgagt gtagaagtcg atgcaaccga
    20881 agtgtacaaa gcgcttaggg aagtaaaagc aaacgttcaa cgagtggaaa gcccagcact
    20941 tagaaaagct ggggagtacg ctcaagaaga gttacgacaa aacacacctt actgggatgg
    21001 aacgaagtca aacggtaaac gtggttcgta tatgcaagaa catgctaaga accatgtggt
    21061 tacaagctcg ataaaaaacg gattggtaga agtcggctat gacaaagatg tttcttggcg
    21121 gatgcacttt atcgagttcg gaacaatcaa acaacgtcca aaaggtttcg tacaaaaaac
    21181 acaaaagcaa atcgaaaaac aagtaacaca aatcattgct gacgaagtaa aaaggaggct
    21241 aggactttga aaacggcagt atcacaagtc tattcaattc tgaatagcaa tgaaaaaaca
    21301 aagaacattg atttttacac caatagtgtt ccggaatcag ctcaaacagt acctagcctt
    21361 ccagttggca gaattacaga gatatccggc aactatgaag attttgcaag caacaatcct
    21421 ttgaccattc aatttaacgt acaggtagat gtatgggtgt caaccatgaa agaggttgat
    21481 gccttttatt ttgcccttga tgaggttatg agggggaatg gttggcaatg cgcatacacg
    21541 gaacaaacag atgacgagga cttggaaggt gcaaagcgga ttatcaaacg atatgtagca
    21601 aatatttcac taaattaaaa ggagagaaaa tagatggcaa cagtaggatt cgagagcgtc
    21661 atttttggcg taaaaacagg tgcaggcggc actctaaaag aattagtagc agataagtcg
    21721 aaaggcggag cgatcgaagc taaaattact ggattaggcg caacttctaa cacaacatac
    21781 gcttcaaacg taccgttctt cattgcaagt aaaggggttt cgtcgccaaa agttacgctt
    21841 gacgtggcag acttaatgga taacggcatt tacagcgaaa tcattggtgc taaaaccgtg
    21901 gatggtgcaa atgtaattgg ttcagaaact gaagcgcctt acgtgtcggt agtcatggtt
    21961 acagcgaaca aagaaggaaa acgcttgttc atgggattga caaaaggaaa attcagtcat
    22021 ccagatatcg acatgaaaac agctgaagac aaaggggtag aattgcaaac cgattccatc
    22081 gaaggggaat tcatttctga tgaacgtggc tatgtatact taacagccgt agaatcagaa
    22141 ggaatgacct tacaaaaatt caaggacttg gtaaataaca aagaggggga gtagttaacc
    22201 ctgcatctac accaacgaca gatacaggga caccaaaaga accagaacca aaaattgata
    22261 cacaaggtta gccatttttg gctagcctta ttttttgtaa aaacaaggag gaaaacaaat
    22321 gattgaattg caattgaaac ttgacggaaa gaaaaaaaca ttcaaacaac aagatatttc
    22381 cgcacgtgca atgcgtgagt gtatcaaatt ttacgagaaa gcggaaaaag cagacctaac
    22441 tgatttagaa gcgattgatt caatgattgc aattacagca gatattttcc aagatccagc
    22501 agttacattt gatgctattt tagacggttt gactgcgagc gagttagtac cggcattaga
    22561 aagtgttttt gaacaaataa atgaactggg aaacaatgaa aaaaagcaga cggcgagcaa
    22621 aaagaaataa gtttttctga agctaggaaa gcaatggatc aaatctacaa agatttaatc
    22681 gaagcaggtt ggacgatgag agatgtggac gaagccgact atcattattt gttacacctt
    22741 tttggagaag tggagagtgg cgaagaatat gtagatggtg ctgatttcat caaacaattt
    22801 ttatcggctg aagacttagt aaaacttgag gaaggaggta aataatggca ggaaaaggac
    22861 aaccggcagg aaatatcaag ctagggatta gtttagatag cactagtttt ggtaacacgc
    22921 tggacgaaat caatgcgaaa gtcaaacaag ctgagtcgaa tatgcgtgcc aatctaaagg
    22981 cttatgattc agcaggacgt tcatacgaag cacttagtca aaagacgaaa gacttgtcta
    23041 cggttatgga agggcaaaac gccaaagtaa gagaattaac aaagcgccgt gatgaagcga
    23101 ttagcaagta tggcgaggaa tcgaaacaag ttgctaacct taacacacag ataaacaatg
    23161 ctaccgcaaa atataatgct tacagtcgcc agttgaacga cacaaaaaaa gaattggtgt
    23221 attccaaaac agccgtcaat gatttatcta atgaaatcaa agaaaatgaa cgacaaatga
    23281 acgccgaagt aaaagcgttg aaagccgctg gtgatgaatc tggtgctttt gaagcaaaac
    23341 aaaaagggct agccaaacaa acggaattat ccgagaaagc tatcgaagaa cagcgcaaag
    23401 ttgtgaaact gatggctgat gagtttggcg attcagcaaa tgaaaccgaa gatgcaaaaa
    23461 gggcattaga aaagttagaa cgacaaagcc aaatatctag caggcaatta gaagcactca
    23521 aaagctccag cgatcaatca ggaaaagaaa tagaagattt tggcgacaag tccacaaggt
    23581 cagctaggaa actggacgga ctaaaagaca aattaagctc gctaaaaagc gcattttcgt
    23641 ttggtgcagt tgctggatta gcgcataacg ctattagcag tgtagtaagt ggcgtgcaag
    23701 gtttggttgg cgaagcagta aacgcatcgg attcattgat gaagttttcc aaaaccatgg
    23761 agtttgctaa ctttgggaag tcacagatag aaagctcgaa aaaagaaatg aaagactacg
    23821 ccgataagac ggtttatggt ttagaagaaa ttctgaacac aaccgcacaa ttggcatcta
    23881 atgggattcc taactataca gaactaacca aggcggcagg taacttgaat gccgttgcag
    23941 gcggttctag tgatacattc aaatccgttg ccatgatgct aacgcagacg gcaggagctg
    24001 ggaaactaac aactgaaaac tggaatcaat tagcagatgc gataccgggt gcttcaggat
    24061 tgttacaaga cgctatgttg aaaaacggag cttatacagg aaacttccgt gatgcaatgg
    24121 cgcaaggtca aatcacttcc gacgagttca accaagcaat tgtacagtta ggtatgaatg
    24181 acggagcagt taaggcagcc acttctacag acacattgag cggttcttgg gagcagatga
    24241 aatccactgt aataaatggg ctacaaagta ttatagaaaa aataggcgtt gaaaatatca
    24301 ctggtttcat caacagagta acaaaaggga ttgaaaattc tattcctaaa attactcaat
    24361 ttataggttg gttgagagat attggaacgt ggatcgttga aaatagagag ccactaacat
    24421 ggattgtcgg aatcataggc ggaattacat tagcagtaaa agcattgaac gtagcaagta
    24481 tgttgctggc aattactggc ggaacattgg cagccccttt tgtggcgatt ggtgtagcat
    24541 taggcgcact agcaggtgct ttggtgatag cttatacaaa atctgaaaca tttagaaata
    24601 tagtcaatgc ggcttttaca gctgtgaaaa acgtagttat gagcgttatc aataatttgg
    24661 tggaatacta caaaatgttg tggagcgtgt tgcagtggct ttgggaaaaa ataaaagaat
    24721 gggcttcatg gattggcaat aaattcattg aaatgaagaa cagcgttgtg aatacggttc
    24781 agaacctatg gaatggcgtg aaaaacttct tcagcaatgg cgttggagat acgtggaata
    24841 aggtagtcgg ttgggtaaaa aacattttca acaaagcaac tgaattgaag aacaacgttt
    24901 ctgatgtaat cggtaacctg tggaatggta tcaaagacac attccgtaga ggtatcgata
    24961 cggtattcaa ttggttttca gaactaccaa cgaagatgaa gaatgccatt attggcggta
    25021 aaaacgccat tgttgatgcg ttcaaaagta ttttcaacgc agcacttaaa gcgataggta
    25081 aaccagttaa cgcaatcatc catggagctt catgggtact agaaaaattg ggcgcagaac
    25141 ctctacaaga atgggatgta ccacaatacg ctacaggtac accagcaggt gggcacccaa
    25201 tcaatggtcc aatgatggtt aatgatggac gtggagcaga aacagttatc acaccagatg
    25261 gtagagcctt cattcctaaa ggacgcaatg ttgttctaaa cgcaccaaaa ggaacgcatg
    25321 tcttgacagc agaagagacc gcgcaacttc aaggttctaa agctcctaag tatcgttaca
    25381 aaaaaggtac taacttcttt ggtaacatgt gggatagtgt gaaaaaggtt gctggtaatg
    25441 taggtcacac aattaaaaac gtagtaggtg acgtgtggga ctttatttca gacccgggag
    25501 cgttagctag aaaagtactt ggggggttag atgtattagg tggattgaca aagtatccat
    25561 tagaagtagg taaaggcatc ctatctaaag caacaagtgc actgactgaa aaaatcactg
    25621 ggttgttctc atctggtaac ttagatacct ccgtaggaac aaatggcgtc tataaatatt
    25681 tggctgatgt tgctaagtct gtgatgaaga aattcccagg atttgtggca actagtgggt
    25741 atagaccagg tgacccttat tcacatggta aacgtaatgc cattgatatt gcattaccag
    25801 gcgtcacagg aggctcacct agatacacag aagcagcaaa ctacgctttt gacaaattcg
    25861 cttccaaaat tggttacgta atcactaatg ggaaagttcg tgaccgttca ggacaatcag
    25921 gtcaaccagc aactggtgca tgggagccat ggcccgatgg agatcactat gatcatgtgc
    25981 atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc aggagatagc gtgggaggca
    26041 gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt gaaaatgacc ggtcaataca
    26101 gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac agagtcaaat ggtaatccta
    26161 atgcagttaa caattgggat attaacgcca aaaatggaac accatcaaaa gggttgctcc
    26221 aagtgattga cccaacattc agacagtatg caatgccagg attcaacagc aatatttttg
    26281 acccactatc taacatctta gcttcaatca gatacgcact atcaagatat ggctcactaa
    26341 caaatgccta tcgtggagtt ggttacgcaa acggtggaat tgtaaaccaa catcaaattg
    26401 cggaaatcgc agaaggaaac aagccagaaa ttattattcc gttagataag gctaaacgat
    26461 caagagcgat gcaattattg tctatcgcaa tggacaaact aggagtttct cctaaaactt
    26521 atggaaatgc gacaactgtt tccagtgatt acgaaatcct agaagccatt gaaaaacagg
    26581 cggcaacaac gaatcagctg ttatcgttat tgctggcatt tttcaaagga aatagccgaa
    26641 ctgatagaga tttagcttta gatattcaga agatcttggt taggaggatg tagatgcgaa
    26701 ctgtattact gagaaataga ataaacgaag aaattgattt gtccacagaa gactattttg
    26761 ctactgggtt gagtaatatg gggtttgaag taaaaaaaga acatgtggga cagtggggga
    26821 atttcagaga aagcagtgag agcgttgaaa tatctgaatt tcagtcatct gtaatcattt
    26881 ctgtgcatgg gtttcgagaa aaagaactgt acaattcact tgtgcagttc ctatcggaag
    26941 gtccgtttga actggaattt gcttttgacg gcgaaacaat ggtaagaaga tgcagtctaa
    27001 aatctttaag taagactgaa atcgatccga aaacatcgtt actaacagac actttagaac
    27061 tatatttcac atcaaactgg tattccgtaa aacgcgaaaa attaattcag cgaccgaatg
    27121 tagtgaaaac acgtggtaag gtttttccgt ataaaagatc ttatatctac actcaaaact
    27181 tgtgggagaa aaaaggtgta ttcaaattca ataataattc tgtataccta acgaatagca
    27241 aggaacggat gtccccgcta aaaattcgtg tgattgggaa gtgttcaaat ccgtattggg
    27301 aagtaatcca aaactcacaa atcatcgcaa cggatggata cttcatagat atgactgaaa
    27361 cacagactct agaagtatca agcctttttg aagatacaac ggcgatttta aaagatattg
    27421 caggggtaga atcttctgtc taccaacaac aggattatac aaaaactaat tttgttcagg
    27481 ccccaactgg agaattcagc attgtgtttc atgttggggg ggcagacgta gagattgagt
    27541 tatacgagga gcgtgatctg ttttgatttt agcagtcacg ctttttcatc gtgatttaat
    27601 gctttataac gaacatttat tctctaacag ttttgaattt ggtgtggacg agatcaacga
    27661 agaggctagt agctttacga tggataagta cgttcctgta aaaacaggcg attttctact
    27721 agcaaaatat attcctagtg gaaaatttgc atattttggt gtaattacgt cgcaggaaga
    27781 cgaaaaaatc agttgtaaaa gcttacttag tttagctgat agtgagatac cgactgcacg
    27841 tgtgtcagga gataactacg aagagcatat ccggcggttg attgaatact atttgctgaa
    27901 tgatccaacg aaacaactaa aagatatttt agacgtcaaa gcagaaagtg cgacctctca
    27961 ttcgtatcaa gctaccgata cgaataaaca caagttaagt gcgtatattc tcaatggttt
    28021 caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa aacagaaaaa tttatacagg
    28081 catacgtgct gtaaatgaat caatttacat taaagacaat tcttctgaat ttagcgattg
    28141 ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac aagctattaa tcgttgataa
    28201 agcaatgaaa gatatagaga aaccgataat actgtcaaca tggtatttgg acgaagaaaa
    28261 caatttgaca caagatggat cgaaagaaaa tatcacgaaa ccaactgtca atttggttaa
    28321 tatctacgat caaaccgcag aagataaagc atcctacgaa gatgtggcaa aatcagagtt
    28381 gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt gtaagaaatg caaaaaattt
    28441 gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt tcttataaag gaaagatata
    28501 caagtcggtt ttgacagctt ggcgaatatc aagtgataag gagtttgtgg aattgacttt
    28561 cggaaacata agaagtcgtt ttatggatta ttttgaagat aatgggggat aaaaaatggt
    28621 tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta agcgcagaaa atgatgctag
    28681 actttatcac gttttatata atcggaaaaa tcaggttatt gatggttacg atcagtctat
    28741 gaatttatct tcaagcggat taacagtaaa agttgctgca ggagcagcga ttattcaagg
    28801 tcgtatggtc gttgttcgac aagaagaaag tataacagtt ccagcaaact caagtggtta
    28861 tatagcatta acagtggatt tgacacaaga agttatacct ggatctattc ttccagaatc
    28921 ggaagaatat gaatggacta ataatcaagt caagctagaa tttataacaa aagttataaa
    28981 aggtaattta aacaatggtg ataaggtcta taatttacca ctatgctcag ttaattctac
    29041 tggatcaact gtttcaatct caaagatatc ggatagttac gagctgactc tctctaaagg
    29101 agaaattttg tggaggggga ctgcgttaat gcatgatact caaactgtcc aaccttcaaa
    29161 aaaaatttgg cagacagttt caggtttttt gttagtatgg ctcccatacg aaaacgggca
    29221 agcaattgag gatagatatg taactacgcc tttttataag gagcgtgtaa cttttactaa
    29281 tgttttagga gaaattgttt caggatttga tgactatcac aaaaagtggt ttagtaaacg
    29341 aataaactat aattcgaata ctaatatatt tacaggtgca gcaagcaatg caagtggaga
    29401 taacgcaaat atggtgctta ggtatatagt ttctttttag ttaggaggta tggaagagtg
    29461 gcaaatttag aaattaaatt atctgcaaat aagagacagc cttatctacg tcaccgtgtt
    29521 gttggtagag ttggtgatgg ggggctcaca acaatcaatg tacaacttct tgaggaagat
    29581 gaaattacac cttttgtaat taatctaaac ggtactttga aatttgtggg cgaagtttca
    29641 aacggtaact ataccgaggg agaaccagaa ataatcgatt cgactaatgg gttaattagt
    29701 tacacgttca ctaagtcaaa tttcagcacg agtcatcaat tcaaacaagc atattttgaa
    29761 tatgtagatc ctaacggcaa aaaagtaact tttcagaact tcatcataga cgtgttagaa
    29821 cgagtggata ttaattcgga gcaagcgaaa tactatattt cttcattgga aaaattacag
    29881 agtgaaatgc aaaccacttt caatcagttc attagtgata aacaggtcca atacgatcaa
    29941 atctactcga aatataacga attagtaaga ttgataaatg aatcagataa gcaagtaaat
    30001 gatcgtattg atcaaaccaa tcagcaaatc ggcgatctcg gcaagctgaa aaagatgtac
    30061 agtaacagca tcgactttgg gggctatgat tattcgggga atccgaattt gttaagtaag
    30121 ctatcatacg acttaattga aaatcaaaat acttcagctg gaacactttc taagggtgaa
    30181 aactcgttta aatataataa gatatcagct gaagtggaag gtggagtaga gttatattat
    30241 aaacgaagag gtatagctaa ctggttaccc tctaataaaa cgcttgtaat gactgttaag
    30301 cttagagctg gagcggacta tagtccagtt gacggaaaac ttatactgat tagatatagg
    30361 tatgttgaca gtggaactgg caagattgtt ttagacttac ctattaacag taattcgata
    30421 actcaggaat ggaaagagtt tagtattact ggaactactc caacatttag cccacaagca
    30481 taccatcctt ggatacaatt tagggctcaa gatggggtac ttggggaaat agaaatgagc
    30541 tatgacatca aaatcgaaga aggctctaca gctacaccat atcagcctaa cttacttgat
    30601 gactcttact ggctaggtaa aacaccgttg ggtgagaatt tgataacgaa tgccaagttc
    30661 ccaattataa cacgaagcaa tcctatttct ggttttgata tttcagaaga attaataata
    30721 ggtgaaactt atacggtatc tttaaaaggg actaagccag ctactaaaga attccactta
    30781 tactacggag aagcaaatta ccagcagttg cagtatcagg caactttact tcctgtggaa
    30841 ggattaacag atgtgtggag tgccactttt acagccagaa atactagtga aacgacaaat
    30901 ttacttagag tagcgttatg gcaaaaacct aattctgcaa catatgatac tgttcaaatt
    30961 gactggctca agattgaaaa aggcgacaca cgcacaccaa atatcagtca gtttaaatac
    31021 tttggtgaag gcttgaaaga cagtaacaat ccgaacgact acagctggga tgtcacacca
    31081 gagtatactg aaaaaagctt gaataatacg gttagtctga ctgaaccgca atccatcgaa
    31141 ggattgaaaa atttcgaaga tggtattcaa tcaaaaggaa aatctgtatt gacatcagac
    31201 gacaacaaat atgaagtcgt aaccttaaca gttacaaacg ggaataccgg atcagcaaag
    31261 ctttatcgtg aaggaaaaac cgtcactatt tatttttttg cgttgaatgg gaaaagcagt
    31321 ggtggaaatg attcaacgat actaacaatt ccagaaggct atcggccacc aattagtttt
    31381 gagcaactgg ttggctcgat agaccgttct actttgaaca gtgctcagtt atctattggt
    31441 gcagatggag ccattaaatg gcgaagaaac tcaagttatg gatcggatta tacctttgca
    31501 attacttaca cgatttagaa aggcgtgaat cgatatgaag gcagcatata gaccaattga
    31561 accttacgga ttcgagcaaa tcattgtgaa tgatgaagaa catttaccgg aagaatgcac
    31621 agaagtcgaa ccaccgattc caaattggaa accgaaattc aattactggg agggaaataa
    31681 atgaaaaaca tttggaaata tggccgtact ggcggagagt atgcaggaaa agtattggac
    31741 gacatgcttg tatccgttcc ttacacagat cagccaccgc tcgaaggggt tcgttcagat
    31801 ggcgaaccgc taacgattgc tgatcagatg tttgatccta aattgaacca atggattatt
    31861 ttagcgaacg cgttagatca caacgattta aacaatctca aagcgatgta cgaggctctt
    31921 gaacatgaaa acggcaacct aaaacagcta aatgccaaac tcatgctaag cgatgtagcg
    31981 attaaacagg aaaatactgc attgaaagaa aaagctgaca gtttagcaca aatcaattca
    32041 aaaatgatgc ttgcttcgtt acaaaatagc aaagacattt cagaaattaa agagcaacta
    32101 aatccagctt caaagggagg tgagtagtat gtttagtttt agcgatgtga aaatgatgta
    32161 tgattggggc tgttttactg acgatcaagt tcgtctattc gttccactat gtatcacaga
    32221 tgaagaagca gatagaatta ttagcaaaga agagagcgca tcttaagtga tgcgtttttt
    32281 tgttggaaag ttggtggaac atgaaagaag aagcgctcca agacgttgtg gagagattag
    32341 taagaattga aacaaaatta gacaactacg aatcacttag agaaaaagct gatagtgcaa
    32401 aagatttggc agacaaagcc tactcagtag cgctaaacaa cgcagaagat atcaaagaaa
    32461 tgaaagccaa taataaatgg tcgtggggtt acatgatcgg tttaggcatt acgatcattg
    32521 gctatttctt gactaaattg taaaggagtt aagaagaaat gattttaccc gataagtatt
    32581 atcaagtcat taaatggacg gttttaacag tattaccagc tgcatctgtt ttagtagcca
    32641 cgttaggaaa agcgtatgga tggaatggaa cagatatgac agtactcact atcaatgcag
    32701 tagcaacatt tttaggcgtt atcactggtg tgtctgcata taacttaaaa aaataggagg
    32761 aaaaaaatga agaagaaaat cattttatca ttgagcctaa taatggctct ttttttattg
    32821 ccaattaacg ggttcgccta tacgatcaac aatgaattta atttaggtgt aaatgaaggt
    32881 agctcacaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg
    32941 acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat
    33001 attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc
    33061 gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat
    33121 aaagtaacgt ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga ttccgctaat
    33181 aaatacggta ttccacttac attagacaca ccgtataatc aaccaggaat caaatcacat
    33241 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
    33301 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
    33361 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
    33421 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
    33481 aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg
    33541 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
    33601 gtaaatcaag cttataatac ttctggaaac gttggctatc atgtatcttt taacatgcgc
    33661 aatttcccta ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc agaaggaaac
    33721 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
    33781 taaaaatagc tcctcgttga ggagcagtac atagaattga aaattaacgt taaatcaaaa
    33841 aaatatttac aagatgttta tttaagtaga taataaaaga aaaaagcagg tgaaattgtg
    33901 acagcagaaa ttggtattat gaataaaagt ggaatcgtgt tagcttcaga ttcagcatct
    33961 acgattggag atagcaaagt atataatact gcaaaaaaat tatttacttt ggattccatg
    34021 cattctgtag gtatcatgat atatggtaat gctgaattta atggtattcc ttgggaaata
    34081 atcattactc aatataagaa aagcattggt agttctgttt ttaatacttt ggaagaatac
    34141 gcggataatt ttatagaatt tgtcaaaaca gcatctttta ttagaagtga acagaccgag
    34201 caagaacaaa tgattggggt ttttcaaaaa ataatttcag gtttatttga gagtatagaa
    34261 gtagatatta actttttaat aagccaaggt acacaaatag acaaagatgt cctagtgaag
    34321 ttattacaaa caaaaatgaa taccaatttg tctcaacagt cccagacatt cattttagat
    34381 attgaaaagg cgttatttct tagtaactat ggagaaatat taaaagatat tcttaatagt
    34441 atttctacaa tggaaggtgt ttcagaagct atatcagagg aaattcaaag ttacgtttat
    34501 gaaattataa ttagagacga tgtttattct tcccctacag gaatagttat agcaggatat
    34561 ggaagaatgg atattttccc taaactatat tcttataata tgtttggatt tgttatgaat
    34621 attctgaaat actcagaata tgaaagtgct caaataggaa atgacaatgg ttcattaaga
    34681 tctactatat taccatttgc tcagtctgat gtggtaaata ctgtagttca aggggtagat
    34741 ccacaaatta ctaattatct gtcgagtcaa gtcgatagtt ttgacgataa cggaaaaaat
    34801 acctatatta atattataaa gaatatttca gagtttcaac aaaatcaatt catatttccg
    34861 ttactaaaca tgattgcttt actcccagtt gaagaaacag caattatcgc agagacttta
    34921 ttaaatttaa ccagttttaa acgaaaatat actacttctg tagaaacagt aggtggccca
    34981 attgatgttt tagctattac acctaatgat ggccctattt ggattaaaag aaaacattat
    35041 tttgatattg ataataatat tggatacaga ttgcgaaagg agagagcgaa tgattacaat
    35101 aattaaacct caggaaaaat cccctgtttt gaaatttaat gagagcaatc tagcccataa
    35161 taaaatgagt ataactgata cagtatttga tgtaatggtt gagcaacaaa aaaaaattaa
    35221 taataacaat agacaaagaa aagaaattaa ccaatattaa tgttttttct gttgcgtttt
    35281 ttgcagtcat aatttaaaac ctcgctcaat cttgggcgag gtcttttttt atttttctga
    35341 aaagttaaag taaataatag cataaaataa tatattttac aaagaataag tacaatctag
    35401 ttttttgcta ttaaatgtgt aataataaat atgccatcac aacatgaaga atgaaatcca
    35461 ttattatcta gtctagtcca ttctttttgt ttgcagtatt tgtgatggct ttc
//
LOCUS       DAZKMK010000016        35017 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000016 DAZKMK010000000
VERSION     DAZKMK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.016"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1917
                     /gene="efbA"
                     /locus_tag="ACG8V1_000860"
     CDS             211..1917
                     /gene="efbA"
                     /locus_tag="ACG8V1_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0328974.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2075..2977)
                     /locus_tag="ACG8V1_000861"
     CDS             complement(2075..2977)
                     /locus_tag="ACG8V1_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0328975.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2974..3666)
                     /locus_tag="ACG8V1_000862"
     CDS             complement(2974..3666)
                     /locus_tag="ACG8V1_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0328976.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3709..4350)
                     /locus_tag="ACG8V1_000863"
     CDS             complement(3709..4350)
                     /locus_tag="ACG8V1_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0328977.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4388..4702)
                     /locus_tag="ACG8V1_000864"
     CDS             complement(4388..4702)
                     /locus_tag="ACG8V1_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328978.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4690..5319)
                     /gene="pyrE"
                     /locus_tag="ACG8V1_000865"
     CDS             complement(4690..5319)
                     /gene="pyrE"
                     /locus_tag="ACG8V1_000865"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0328979.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5326..6042)
                     /gene="pyrF"
                     /locus_tag="ACG8V1_000866"
     CDS             complement(5326..6042)
                     /gene="pyrF"
                     /locus_tag="ACG8V1_000866"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0328980.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(6039..6971)
                     /locus_tag="ACG8V1_000867"
     CDS             complement(6039..6971)
                     /locus_tag="ACG8V1_000867"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0328981.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6968..7744)
                     /locus_tag="ACG8V1_000868"
     CDS             complement(6968..7744)
                     /locus_tag="ACG8V1_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0328982.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7798..10983)
                     /gene="carB"
                     /locus_tag="ACG8V1_000869"
     CDS             complement(7798..10983)
                     /gene="carB"
                     /locus_tag="ACG8V1_000869"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0328983.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10988..12064)
                     /locus_tag="ACG8V1_000870"
     CDS             complement(10988..12064)
                     /locus_tag="ACG8V1_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0328984.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12069..13349)
                     /locus_tag="ACG8V1_000871"
     CDS             complement(12069..13349)
                     /locus_tag="ACG8V1_000871"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0328985.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13368..14294)
                     /locus_tag="ACG8V1_000872"
     CDS             complement(13368..14294)
                     /locus_tag="ACG8V1_000872"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0328986.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14307..15590)
                     /locus_tag="ACG8V1_000873"
     CDS             complement(14307..15590)
                     /locus_tag="ACG8V1_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0328987.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15604..16143)
                     /gene="pyrR"
                     /locus_tag="ACG8V1_000874"
     CDS             complement(15604..16143)
                     /gene="pyrR"
                     /locus_tag="ACG8V1_000874"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0328988.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16378..17289)
                     /locus_tag="ACG8V1_000875"
     CDS             complement(16378..17289)
                     /locus_tag="ACG8V1_000875"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0328989.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17286..17750)
                     /gene="lspA"
                     /locus_tag="ACG8V1_000876"
     CDS             complement(17286..17750)
                     /gene="lspA"
                     /locus_tag="ACG8V1_000876"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0328990.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17755..18453)
                     /locus_tag="ACG8V1_000877"
     CDS             complement(17755..18453)
                     /locus_tag="ACG8V1_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0328991.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18440..18826)
                     /locus_tag="ACG8V1_000878"
     CDS             complement(18440..18826)
                     /locus_tag="ACG8V1_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0328992.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18973..20643)
                     /locus_tag="ACG8V1_000879"
     CDS             complement(18973..20643)
                     /locus_tag="ACG8V1_000879"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0328993.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20826..21059
                     /locus_tag="ACG8V1_000880"
     CDS             20826..21059
                     /locus_tag="ACG8V1_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0328994.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21158..21589)
                     /locus_tag="ACG8V1_000881"
     CDS             complement(21158..21589)
                     /locus_tag="ACG8V1_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0328995.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21668..22081
                     /locus_tag="ACG8V1_000882"
     CDS             21668..22081
                     /locus_tag="ACG8V1_000882"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0328996.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22119..23267)
                     /locus_tag="ACG8V1_000883"
     CDS             complement(22119..23267)
                     /locus_tag="ACG8V1_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0328997.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23389..23985)
                     /locus_tag="ACG8V1_000884"
     CDS             complement(23389..23985)
                     /locus_tag="ACG8V1_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0328998.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24185..26827)
                     /gene="alaS"
                     /locus_tag="ACG8V1_000885"
     CDS             complement(24185..26827)
                     /gene="alaS"
                     /locus_tag="ACG8V1_000885"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0328999.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27215..27646
                     /locus_tag="ACG8V1_000886"
     CDS             27215..27646
                     /locus_tag="ACG8V1_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0329000.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27691..29040)
                     /locus_tag="ACG8V1_000887"
     CDS             complement(27691..29040)
                     /locus_tag="ACG8V1_000887"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0329001.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29110..30273)
                     /locus_tag="ACG8V1_000888"
     CDS             complement(29110..30273)
                     /locus_tag="ACG8V1_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0329002.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30402..30725
                     /locus_tag="ACG8V1_000889"
     CDS             30402..30725
                     /locus_tag="ACG8V1_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329003.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30908..32257)
                     /locus_tag="ACG8V1_000890"
     CDS             complement(30908..32257)
                     /locus_tag="ACG8V1_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0329004.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32326..33279)
                     /locus_tag="ACG8V1_000891"
     CDS             complement(32326..33279)
                     /locus_tag="ACG8V1_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0329005.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33381..34571)
                     /locus_tag="ACG8V1_000892"
     CDS             complement(33381..34571)
                     /locus_tag="ACG8V1_000892"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0329006.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34771..>35017)
                     /locus_tag="ACG8V1_000893"
     CDS             complement(34771..>35017)
                     /locus_tag="ACG8V1_000893"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329007.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9969 a         7649 c         6130 g        11269 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgataccta
       61 aaaatttgga tttcaacgat agaaacactg ttttttatgt aattagtcca agatttttct
      121 agacttttgc tttactattc ggccaaattg gtttttcttc tttttttcgt tataatgaat
      181 atacttatga aaagaaatga ggaatgaacc atgtcctttg atggtgtatt tacccacgcg
      241 atggtcaacg aattgcgtga aacgttacta tctggccgga tctcgaaaat ccatcagcct
      301 tatgaaaatg aaatcgtgct ggtgattcgt tcacgcggca aaaaccatcg attattatta
      361 tctgcgcacc caagttatgc tcgtgtacag attacccaaa tcgattatca gaatcctgat
      421 aatccgccta acttcgtgat gatgcttcga aaatatttgg atggcgctat tttagaagac
      481 atcgaacaaa tcgaaaatga tcgagtgatc catttccact ttgctaaacg aaacgagttg
      541 ggtgatctac aaaatatcat attgatcgtt gaattaatgg gacgtcatag tacgatcgtc
      601 ttagttaatc gagaaactgg aaaaatactg gatgcgatca aacacattgg aagctctcaa
      661 aacacttatc gttctttgct tcctggtgta gaatatgttg ctccgccaaa acaagaggtc
      721 ttgaacccat ttagctccga gaaagaaaaa atctttcagc ggctttccca aactgaacta
      781 gatccaaaag caatccaacg tcagtttcag ggaatcggtt tcgatatcgc tcaagagctg
      841 accaaaagat tactggaacg tcctaatgaa aagatggttg tatgggacga atttttctca
      901 gccatcagcc atcagcctat tcctactttt tacgaaacgg aaaataaaga ttttttcaca
      961 ccgattgctt atcaggtatt gtccgaacag gcatctgctg taacagctta tccaacactc
     1021 agtcaattat tagatagcta ttatcacgaa aaagcagaga aagatcgggc aaaacaacaa
     1081 ggcggagaac tgattcgaaa aatcgaaaat gaactaaaac gaaataaaaa taaattgaaa
     1141 aaacgtgaac aaacactcaa agaatccgaa aacgctgaga attaccgtag aaatggcgaa
     1201 ctacttacta cttttctgac acaagtacca cgcggcgcaa aagaagttgt tttacctaat
     1261 tattatgaag aagatcggcc aatcaaaata gcactagatc cggcattgac accaaatcaa
     1321 aatgcacaga aatatttcca tcgataccaa aagctgaaga acgctgttaa gctgattggc
     1381 gaacaaatcc aagaagccaa agatgaaatc caatatcttg aatctgtctt atctcaactg
     1441 gaaattgccg gcccaatgga tatcgaagca attaaagaag aattgacttc agaaggatac
     1501 ctgaaaaaga agaacttaaa aaaacaaaaa cgtaagaaac catctcagcc tgatcaatac
     1561 ttttcttctg acggaacgct cattcttgtt ggtaaaaaca atcttcaaaa tgaccagctg
     1621 acaatgaaga cagctaaaaa aaccgactat tggcttcatg caaaaaatat acccggttcg
     1681 cacgtcatca ttaaaagtga caagccctca gatgaaacaa ttacagaagc tgccgagtta
     1741 gctgcctatt tttcaaaata ccgatactct gcccaagtgc cggttgatct tgtacaggta
     1801 aagcatatcc gaaaaccaaa tggcgccaag cctggttatg ttatttatga aaaccaaaag
     1861 acgattatcg tgacaccaga agaagaaaaa attacagcta tgaaacaaaa caggtaatca
     1921 atgagtgttt ctagaatcat atttgcgtac tttcccacaa cagcaattag ttataaattg
     1981 tttgctacaa aaagaggagt tgtgacaaaa ttctgtcaca acccctcttt tttgatcaac
     2041 ggtgttcaat aaacagcgtt ctcatctgct tttcttagct ctgctggtcc agtaaccgag
     2101 taagctcttt gataaatctt tgagcgattt cactgagcgg acgtttcgcc gaataaatga
     2161 tataaaattc atttagttcg ccttctgcca aagggattaa ccgcaagtgc tcgcgaatcc
     2221 ctccaatcac aataccagat cctgaagcgt aagcatcagt ttcaagaacg atattcatca
     2281 aagtacctcg atcactcgta taaatcactg tttcttgatc agggatctcg ataaggtctt
     2341 cataaaaata cgggaaatta tttccatcct gtgtaaaacg aacttgagga taagataaca
     2401 attcttcttt ggtgatctct gcgtgttcag ctaatggatg tccttgcctt aaaaaaatat
     2461 gcgttttgaa ttttccaatg ctgcggccac ttagctctcc ttgttgtaaa tatcttctga
     2521 taccagcttg attatgctca ttcatataaa taatgccgat ctcactgtgc tgctctttga
     2581 cttcttcgat cactctcgct gttgtcgtct ccatgatccg aaattgtttg atctcgtctt
     2641 tgaattcctc gagtagctgt gcggtcaaag gacctaagaa atcataatgc tgggcagcga
     2701 tcgaaaaatt gtctctcttt tcactcgtta gatagtgagt ttctaatgta tcaagctgga
     2761 ccaatatttt ctcggcacga tttaagaagt cttgcccttc ttctgttaaa tatgcccctc
     2821 gattcgttcg agtaaacagc gtgatcccta actcggtttc taattctttg ataccagctg
     2881 aaagacttgg ctgggagaca aacattttct ttgccgcttc tctgaaacta ccattgtttg
     2941 ctacagcgac cacgtatttc atctgctgaa tattcattgc tcaaactcct cgaaataata
     3001 aatttctctt ccattcaagg gctggattgg tcggttgttc ggtcgagcat actgaccatc
     3061 gaaggggaga agaaactctt ggttcagttt tcctacatga tccattacaa accattggat
     3121 tggcccgctg gtaggcggag ttgtcaatga gccaatataa tggaaatggc ttttttgctc
     3181 cggaaagaac acatccggat ttacccaatg ctcatgtttt tctaagttcc aactttcccc
     3241 attttcttga ttatacagcc aaccttcctg taccattagc tggtacatga cacccacaac
     3301 tagattctct tttttgctgt tcatatgaac aaaatgaaat tcaatatctt cctgttttcc
     3361 gttgatgata tgttcagaag gcaagtggaa atgaatatct gtcaaataat agcgttcttt
     3421 cttgaactct acataactga gttggtcaaa aggtactaaa tgaatcgtgt ttttgaattc
     3481 cttgtcagta aaccgttgct tttgataatg aaaggaaagc gtgtctcctt ctattttggt
     3541 ttcttccccc ttggtcaaat gaataggcga ctgcaatgga aattccgcac ctctcgcaaa
     3601 ccaatcacat aaactcgccc aattttccgg acctttgtct ccagaatagc cccattcgac
     3661 atccatattc tttttcaact atacttcccc ctgcatctca tatcattttt atttttcttc
     3721 tttctttaca atcgttgaga cggtttctaa aaacgcctct tttccataaa attcctggtt
     3781 caatagttcc ggaaatatct ctttaaagaa atactgaaca gaaggccgat gttcaaagct
     3841 tttaatcgta gtcactgcct caatgaacat ctccttataa actttttccg ggttattttt
     3901 gatgatttca tcaaaagatt cataaagaag gtcaacctta tctgaaatgg ctaatagttc
     3961 tccttctacg gtttcatctt ttccttcgaa caaccgacgg cgataaactt cttggaactc
     4021 agctggaatc tcttggttga tgaattcgtc cgtcatcttt tcttcaactg tttgcagcat
     4081 ccctcttaac tctttgcttg cgtatttcac cggcgttttg atatcaccaa taaaacgctc
     4141 tgtgtagtca tgattcaatg atttttcata gagcagtttc caatcgatcg gttgtccatt
     4201 cgcttcttca atgtctccta gaacttgcgc aatagatgct acacgatacg aatgtgctgc
     4261 aactgtatgc tctgtatatt taaaaaatcc aggtgccctt gtgatcgtct ctaaattgtt
     4321 caatcctaaa ataaattcat tcaaccccat aaaagttacc tccctttgag atttcttttt
     4381 ccgaatatca tatcattaaa taactgatat tcgcaacatt tccgctcttt accatttttg
     4441 aaacagctcc cttaccaaaa aatagagaaa atctatatat aatagaaaaa agagaagaga
     4501 ttattctctt ctttctgcta accagtctta gaaaaatgaa ttttaaaaaa atcaaaaact
     4561 gatcacgata gaagtttctt ttcatacctg atgaaaagat gcttctcgca atcagttttt
     4621 ggatttttgc tattccaatt gatgattctg gagacacaaa tcggatttct ctatcttcat
     4681 caagcgcgct taaatctgcc aattttcagg atcttttttc cattctttta gcagagttaa
     4741 ttcttgctca tcgatataac gatcttctaa agctgcttca atcaatacag aataattggt
     4801 caacgtcata agagggattt cggctttttc gaaatttgct tttccttttg gcaactcata
     4861 cgtaaagata gccgcaacac caaggatatc cgcaccttct ctttttgctg cttcagctgc
     4921 ttccaagaca cttccgcctg tagaaatcaa atcctcgatc acaaccatct tctgaccttc
     4981 aaatatacgt ccttcgattt ggttaccttt cccatgatct ttcgccttac ttcgaatata
     5041 gaccattggc aaatccagta tttccgctac ccatgcggca tgagggattc ctgctgttgc
     5101 agttccagca atcacttgta cttcagggta agcttctttg attttgcttg ctaatccttt
     5161 tgcgatctct tttcggactt ttggatagct catcgtgatt cggttatcac agtaaattgg
     5221 gcttttgatt ccacttgccc atgtaaaagg attacttgga tttaaaaata ctgcttcaat
     5281 ttctaataag tcttttgcga tactttgttc aacattcatt ttaggttagc tccattctgt
     5341 ttgaatcgtt tgatatgctt tataaggatt agttgactga gtgattggtc gacctataac
     5401 gataaaagtt gagccgatct tttttgcctg agaaggtgtg actactcttg tctgatctcc
     5461 tgcgactgtt ccttctggtc ttatacccgg agttaagcaa acaaattcgt ctcctgccat
     5521 ttctttgatc gcttctactt cccaagcaga agaaaccaca ccatctaatc ctgctttttt
     5581 ggcacagcta gcataatgga tcacactttt ttctaaagaa acttcgatta gttgatcggc
     5641 atgcatttct tcttcacttg tagaagtaag ctgagtgata gctagaagta gcggacgttt
     5701 tccaccttct ggagtcccct cttctaatcc tcgcattgcc gcctccatca tacgaatacc
     5761 gccagcggca tgaacacagg tgagatcaac accaagcttt gccaatcccc gcattgcttt
     5821 ttcgaccgta ttcggaatat cgtgaagttt cagatctaaa aatacattat gaccagcctc
     5881 ttttagataa cgtacgattt caggtccttc ttggtagaac aattccattc cgattttcac
     5941 aaacaatttt tcttctttcg ggaaaaatcg aagaaagtct tctacctctt gcctagttga
     6001 aaaatctaat gcaataattg gtcgttggtc tagttgattc atcgttcacg ttcctctctc
     6061 acttctttga ttaatctttc cagcgattcg atgcctagtt cgctcattcg cacagggagc
     6121 tctttaatta gtttaggaca gatatacgga tcggtgaaat tcgcggtccc tatagctaca
     6181 gcacttgctc cagccataaa catctcaaga acatcatcca ctgtctgcac gccgcccatc
     6241 ccaatgattg gaaggctgga tatacttgct acttggtgaa tcaaacgaat agcgacaggc
     6301 ttgattgctg gtccggagag tccacctgtt tgattggcta aaatcggttt tcttgtctta
     6361 aggtcaatcc gcataccaag aagagtattg atcatcgtaa acccatctgc ccctccagct
     6421 tcgattgcct gagcgattgg aacgatgtct gttacatttg gggacaattt gacatagaca
     6481 ggaacagaag aaactttctt tactgcttgt gttaattgaa aagcaacatc agaatctgtt
     6541 ccaaaagcaa ttccgccatg tttgacattt gggcaagaaa tgttcagctc gattgctttc
     6601 acatttggtg cttcgccaat tttgctacaa acttctacat agtcttcttc acaagcacca
     6661 gcaacatttg cgatgatggg taactcattg tactcctcta attctggaag aagtttatgc
     6721 ataacaacgt ctagaccagg attttgcaaa ccaattgcat tcaacatgcc agatggtgtt
     6781 tccgccactc ttggcgtggg attgccaaag cgggcatttg gagtcgttgc cttgaccatg
     6841 atcgatccaa gctgattcaa atcatagtat tttccgtatt ctttgccaaa accgaaacag
     6901 ccgcttgccg gcatgatagg attttttaat tctaggccag gaagtttgac cgctaaagga
     6961 ttctcattca tataattacc tctcctattt taaaaatcgg tccttcgtca caaactttca
     7021 cactttttgt tcctgtttca tcttccggcg tatgacaaac gcaagcatag caagccccta
     7081 tcccacatgc catgcgggat tctaatgata actggacatt ttgatgcgaa gaaaacagct
     7141 gttccactgt cttcaataac ccgtttgaac cacatgcaaa aacagctgta ggttccgtct
     7201 tcgttcctaa caataaatta ccgacatttc cgtgcatacc gtatgttcca tcgtcagtag
     7261 cgattcttgt ttctcctaga cgattaaatt ctttttcata ataaacgact tcatgtgtgg
     7321 caaaacctaa aaaatgcgtt ggcttaattc ctttttcgac taactgtttt gataactcat
     7381 atagaggagg gacaccgatt cctccaccga ttataaatac ttcgtctcct gtatgaagat
     7441 tggacaaatc aaaaccattg cctaaaggac ccatgacatc taaatggtct ccggcagaaa
     7501 gagtagagaa gactctagtc ccctcacctt ccacccgata aatgatacgg caagtatttg
     7561 tttcgtggtc gatatgattc aagctaatcg gtcggcgtaa tagcaagtct gcacgcggca
     7621 cgagaatatg caagaactgt cctggttgct gcatttcttt tactagatca cctttcaatg
     7681 tcatttcaaa aatctttggt gccagctgtt tttgctgaac gatttccatc atttcttgtt
     7741 tcatctgtcc cctcctgcaa cggtttttcg tctttttgtc ttaacatgtg ctcgccgtta
     7801 aatagggtta gttgaaaaag cacgggattc catcactcga acgatcgcat ctgctgtgtc
     7861 taaagaagta aacaatggaa taccatgttc aactgcttct cttcggatga tgaaaccgtc
     7921 tttagaagca tttccccgat ctttatcaat cgtattgaca accacttgtg ttttaccatc
     7981 tcttattaag tcgactacat ttttttctgc tgcttcctca gaaattttcg ctactgtttc
     8041 aacgaccaga ccattttttt cgaaataagc agctgtatgt ttcgttccta tcagactata
     8101 gccaatttct gtaaaacgtt tagctaattc tagtgcttct tcttttgttt cgtccgcaat
     8161 cgtaaataat acggctccat attcaggcaa gtgtaatcca gaagcttcga atgccttgta
     8221 caaagctttg tctaattgtc tgtcagatcc cattacttct ccagtcgatt tcatttcagg
     8281 ccctaagtaa gtatcaactt tctgcaattt cgtaaaagaa aagacaggtg ctttaacatg
     8341 aacttctttt gtttctggat aaagtccgtt ttcataacca agtgtcttta gtttctctcc
     8401 caaaatagct ttcgtcgcaa tttgtgccat tggaattcct gtcaccttac ttaagaatgg
     8461 aacagtccga cttgctcgag gattgacttc gatcacataa acctcatttt catggatgac
     8521 aaactggata ttcatcatcc cgacacaatt caaacctaat gctaagcgga tcgtgtaatc
     8581 ttcgatcgtt ttcttgatcg attcagataa tgtttgaggt ggatacacag ccattgaatc
     8641 ccctgaatgg acaccagcac gctcaatatg ctccatgatc cctgggatga gtactgtttc
     8701 accgtcacaa atcgcatcca cttcgcattc accaccaagc aagtaacggt ccactagtac
     8761 tggatgttct ggagatgctt tcacagcatg ttccatgtaa tcttctaaat ctcgctggtt
     8821 ttcaacgatt tccatcgcac gtccgcctaa tacatagctt ggacgaacaa gaactggata
     8881 accaattctc gaagcgataa ccacggcttc ttctttactt gtagcagtat cccctaaagg
     8941 ttgaggaata tccaactctt tcagcgcttg ctcgaataaa tcacgatttt ccgcacgatc
     9001 caagtcttcg atcgttgtcc ctaagatttt tacaccagca gcagtcaaag gttcggctaa
     9061 gttgattgct gtctgaccac caaattgaac gattactcct atcggctgtt ctagctcgat
     9121 cacattcatc acatcttcga aggtcaatgg ctcaaaatat aatttatccg aaatagaaaa
     9181 gtcagtcgaa actgtttcag gattgctgtt catgatgatt gcttcgtatc ctgcctgctg
     9241 gattgctttg acagaatgca cagtcgcata gtcaaattca accccttgtc cgatacggat
     9301 aggaccagat cccagtacta gaattgaggg cttttctgat cgtgaacttt cattttcata
     9361 ttcataggaa ctataaaaat aaggtgtttt tgattcgaat tccgctgcac aagtgtcaac
     9421 catcttatat acaggaatca aatcattttc ttttctcatc tggcgaacgg cttcttcgtt
     9481 tatttcccac agctccgcca cttttttgtc tgtaaaaccg ttttgtttcg ctgttttcaa
     9541 tatcttgtca tccaacggct gctgtttcaa ctgctgttcg atttctacga tatgcaacag
     9601 tttatctaaa aagaataaat cgatttttgt caaatccgct aattcttcta tggtgtaccc
     9661 tcttctaata gcttcagcta cataaaacag gcgatcatct tgtgctcgaa ccactttttg
     9721 gattaggtca tcgtccgtga tgtctgccaa ctcaggaagt tcgatatggt gtgtaccgat
     9781 ttctaaagaa cgcacagctt tcaataaaga ctcttcgata ttccgaccga tagccatcac
     9841 ttcaccagtt gctttcatct gcgtgcctaa aagccgttct ccgttttcaa acttatcaaa
     9901 cggccagcga ggaattttag ctacaacata atctaatgca ggttcaaatt ctgcaaaagt
     9961 cgttcctgta acaggatttt tcatttcgtc taatgttaat ccaagcgcta ttttagcagc
    10021 tagcttagcg atcggatagc cagtcgcctt acttgctaac gcagatgaac gagaaacacg
    10081 cggattgact tcaatcacat aataattaaa actatgtgga tcaagagcga gttggacatt
    10141 acagcctccc tcgatcttaa gtgctcgaat aatcgaaaga gaagcatcac gaagcatttg
    10201 atattcatgg tcagacaatg tttgactagg ggcaaatacg atagaatcac ctgtatggat
    10261 acccaccggg tcaaaatttt ccatattgca gacaacgatt gcattatcag ccgaatctct
    10321 catcacttca tattcaattt ctttaaaacc tgcaatactt cgttcaatca aacattgggt
    10381 agcgggggaa agacttaatc cattttctga tacgatacgt aattcttctt cattttcaca
    10441 catacctcca ccagttccgc ctaaagtaaa ggctggacga acgatgacag gatagccgat
    10501 tttatttgca aaatctacag cttcttctac tgtattgacg atttcagact cggggattgg
    10561 ctgattcaat tctttcatca actgtttgaa tagatcacga tcttctgctt gatcgatcgc
    10621 agaaagcttt gttcctagaa gttctatgtt caattcttct aaaatacctg atgctgccag
    10681 ctccattgcc atattgaggc ctgtctgtcc gcctaaagtt ggaaggagag catctggacg
    10741 ctcttttcga agaatccttg atacaaattc aagtgtgatc ggttcgatat atacttgatc
    10801 tgccacttct ttatccgtca tgatcgtcgc aggattagaa tttactaaaa ccacttcata
    10861 tccttcttct tttaaggcaa ggcaggcttg tgttcctgcg tagtcaaatt ctgcggcttg
    10921 accaatgatg atcggaccag atccaatcac cataattttc ttgatatctg tacgtttcgg
    10981 catctgttta ctgctccttt cctgcgtcca tcatttccat aaattcatca aataaatgta
    11041 gtgcatcatg cggaccaggg gcagcatctg gatgaaactg cacactgaaa gcgggataat
    11101 ctctgtgttt gactccttct actgttccgt catttacttc tacatgagtt acgatgagtt
    11161 tttctggatc aatcgtggct tcatcgactg catagccatg attttgagaa gtaaaatcaa
    11221 ttcttccggt cgctacttct cgtaccgggt gatttaatcc tcgatgtccg aatttcattt
    11281 tataggtatc tgcaccgttg gctaaagcaa ataactggtg accgagacaa ataccaaaaa
    11341 tcggtacttt tccttggatt tttttgatca tttcgattgc ttctggcaca tcttttggat
    11401 caccaggtcc attcgtcagc atgactccgt ctggtgatag cgctaaaatc gtctcggcat
    11461 ctgtgttata gggaagtact gtgaggttac agtttctttt actcaattct cgtaaaatac
    11521 tatgtttcaa gccgaagtca atgacaatga ctttccgacc tgttcctgga cttggataag
    11581 gtttgttagt tgagacttgc tgcacttgat tggttgctag aacggttgct ttcagctgat
    11641 caaatgcatg ttcgaaggaa tctccagcat caactatact agctttcatc gttccgactt
    11701 cacggatttt gcgagtaagg gcgcgcgtat cgatacctga tattccagga attccttttt
    11761 gtttcaaaaa tgaatctagc gtcatttggt ttcgccagtt tgaagctaca cgtgcatgtt
    11821 cttttactac tactcctttg catgttggag aaatcgattc ataatcatct ctatttaccc
    11881 cataattacc caccatagga taggtaaatg taatgatttg accgttaaaa ctttgatcag
    11941 tgatagtttc ttgataacca gtcatacttg ttgtaaaaac gatttctccg aagacactag
    12001 catctgcacc aaaagccttt ccttcgaaaa ctgttccatc ttctaatata agcaaacgtt
    12061 ccatactttt attctccctt ctgccacaca agttttccgt tgacaaaagt catttgtgtt
    12121 tcgcctttta ctttccagcc gataaaaggt gtattctccc cttttgataa aaagtcttct
    12181 ttatcgatcg tgcaagtttg tgtaatatca aaaactgcta catcagcggg ggcaccaacc
    12241 gttagcgttc ctgcattcaa accaaaaatc tcagccggtt taactgccaa ccaattgatc
    12301 acttgttcca acgtgaatcg accagtttct acaaaatgag tatagatcag ttgaaacgct
    12361 gtttcgcttc ctacgatacc aaatggtgat ttcatgaaac tttggctttt ttcttctaat
    12421 ccatgggggg catggtctgt agcgatgcaa tcgatcgtac cgtcaagcaa gccttcgatc
    12481 aaagcttctc tgtcctcccg tcctcttagc ggcgggttca ttttccaaaa gccgaaatct
    12541 tcgggaatat cttcgtcgat caaaataaga tgatggggtg aaacttctgc tgttacgtgg
    12601 attcctgctt tttttgcgtc tctgattaca cggacacttt ctttagtaga aacatggcaa
    12661 acatggtaat gaacaccagt tgcttcagct agcaacagat ctctagcaat ttgagaagac
    12721 tcggttacgc ttaagatccc gggaagacca agttcttctg ccttcttgcc agcgtgcata
    12781 acgccgccaa ataataaaga ttcatcttct gtatgggcta ctagggcttt attgtttttt
    12841 gcagcttctt tcatagccag atacatcgtc ccagctgttt ggacacctac accgtcattt
    12901 gtgaaagcaa aagcaccttc ttcaatcagt gcttcttgat caactaattt ctcacttcgc
    12961 agattttctg tgattggcgc atactgcaat accttcacaa ctgcatcttt tctaatgatg
    13021 tcatatactt gtctcagctt ttctgccgta tcaggtacag gatttaaatt cggcatcgcg
    13081 catacagtag tgaaaccgcc acgagcagct gagcgagtcc cagcttcgat cgtctccttg
    13141 tatgtaaatc ctggctctct caaatgaaca tggacgtcca ctaaaccagg tgtgatcaat
    13201 tgtccctttg catcaaacac ttcatcaaat tcggcttctg agaatcctgt accgattgct
    13261 ttgattcgcc catcttctat ccaaatttct gctggtgtcg tcatattttt tcgtgtattg
    13321 atctgaccgt ttttaatgag tgttttcatt ttttcatcct cctataatta cgcttttcca
    13381 tttagcactg cttctaagat tgccattctg acgaatactc cattgctcat ttgctgaacg
    13441 atccgcgact gtaatccttc cactaatgaa tcagctaatt ctacatcacg gttaactggt
    13501 gctgggtgca taatgattgc atgtttttgc aacttcttcg cacgctcaac tgttaaccca
    13561 tgttcctgat ggtagccttc ttttgagaaa ctttcttttc catcatggcg ttcgtgttgc
    13621 acccgaagca tcatcatgac atcaacttga tctagtagtt catccaatgg catataatgt
    13681 ccgtacactt cgaattcatc gtcgtaccat tctctaggac ctgagaagaa tacttgtgcg
    13741 cctagacgtt ttaacatctg catattggat ttagctacgc gagagtgagt gatatcacct
    13801 acgatggcaa ctttcagacc ttcaaaatga ccaaattctt cataaatggt catcaaatca
    13861 agcaagcatt gggttggatg ctgtccgctt ccgtctcctc cattgataat cgaacactgg
    13921 attgttttac tttgtatcag ttcgtcataa tagttctcat caccatgacg gataaccgct
    13981 acgtctacgc ctaatgctga catcgtcagt actgtatcgt atagtgtttc ccctttttgg
    14041 acagagcttg tgcttgcttc aaattcaatc acttctagcc ctagtttttt ctccgctaca
    14101 tcgaagcttt tatgtgtgcg agtactattt tcgaaaaaga ggtttgtagc aaagtactgg
    14161 cgcttttgcg gagtccaatt agcaccttgc ttgaattcct gccctctgcg gatcaagccc
    14221 ataacttctt gatcagtaag tgcctcaacg gtcaataaat gctttaagct gatacgttct
    14281 gatttgatga tcataatgtt tcctccttag ttttcttcgc ttcgtgaagt ttctggtaag
    14341 ataagattta aaatgatgcc aagtacagtt gctaatgcca ttgccgacaa tgtaaatgtg
    14401 cctacttcaa agacgagtcc gccaattcct actactaaga taactgaagc aatcaacaga
    14461 tttttctttt tatcaaaatc gattttgttc tcgattaaaa tcttcattcc acttgcggca
    14521 attactccaa ataagataaa actgattcct gagatgactg gtccagggat acttaaaatc
    14581 aaagcgctta attttcctac gaatcctaaa atgactgcaa atactgcagc tccaccgatc
    14641 acaaacacgc tatggacacg tgtgatagcc agaacaccaa tgttttcacc gtaacttgtt
    14701 acaggaggac ctccgacaaa ggctgccacg atttgtgcta atccatcacc tgtcaacgtc
    14761 ttatgaagac ccggttcttc aaagaaatta cgtttagtta gtttattcaa taccataaga
    14821 tggccgatat gctctgtcat cgtcacaaaa gcgatgggtg ccattgttgt gattgctcca
    14881 agatatagct ttggttcata ttgtacaaag ggaatctcaa agttaggaac agcaaaccag
    14941 gcagcgtcta cgattggctt agtatccacg attccaaaca gcaaggctac gatgtatccg
    15001 ctaataatac ctagtaaaat cggaatcaat ccgagaaaac ctcttagcca catgttgaag
    15061 aaaattgtta atcctaatgt gatcagcgct acagcaatat acttgaaatc atacgtgccc
    15121 tgattataca tagcatttgt tgctgcattt gccgcaagtc ccaacccgat aaccatgatg
    15181 actggaccga cgacgattgg aggcaagatt ttatccagcc atgctgaacc gatctttttg
    15241 atgatcagcg caacaatcaa atagactaat ccagttgtga ctgctccttg tgcaatcgct
    15301 ggataaccat ctgttttcat caaaagctgc atcgctgcaa tgaaagcgaa gctgcttcct
    15361 aagtacgcag gtatctttcc tttagtagta aataagtaca ccaatgtacc tagtcctgaa
    15421 ctgactagcg caatacctgg atcgatacct actaagatgg ggactaacac ggttgctccg
    15481 aacatcgtga acaagtgttg taaacttaat cctacccaat gtcctggggt tgggcgatcg
    15541 tggatatcta acaccacgtc atcattatga aatccttttt tctccgacat gtttctctct
    15601 cctctaatct tcctctttca aaatcatgat gcgatcttgt ccatcaagct cttgcatctc
    15661 tactaaaatc tcttccgccc gagatgttgg aatgttcttc ccaacataat ctgcacggat
    15721 tggcaattct cgatgcccac gatcgaccag gactgctaat gaaatttttc ttggtctgcc
    15781 aaaatccatt actgcgtcca ttgctgctcg aatcgttcgt cctgtataca aaacatcatc
    15841 aatcaaaata acttcttttc cttctaagga aactgggatg tcagaagaat gaagttccgg
    15901 ttcttctgga ttttcttttt tgtcatctcg gtataaagtg atatccagtt ctcctacagg
    15961 aatatcgata tcttctagct gcttcaggcg ttcggcaatt cttgatgcaa tgtagattcc
    16021 tcgtgttttg atacctacca atacgatatc ttggatacta tggtttctct cgataatctc
    16081 ataagtaatt cgagtcaatg ctcgcttcat ggttacctga tctactactt cttttgcttg
    16141 cattttcatt ccccctactt ttttgcataa aaaaactcct gccctaagag gacaggagga
    16201 atgtttagac tgagcgtata ctgatacact gatagctata ctatgtcaat gagcaatcta
    16261 cttatggcgc accatacgta tccttgctcc cacccttctt agcctcacgg gactaaactt
    16321 aaaggaattc tattaagttt tatgctttcg ttgttaatta ctataccgtt atctaaatta
    16381 aaatgcaatg ttttttctta atttttctaa agttttttca aaaacttctg gaagaggtgc
    16441 ttcaaagatc atcttttcgt ttgtagtcgg gtgcgtaaaa cctaacattt gcgcatggag
    16501 aaactgacca ttgcctttca aagttttctt cggtccgtat actggatctc cagctaacgg
    16561 ataaccgata tacttcatat gcacacggat ctggtgtgtt cttccggttt ctagttttaa
    16621 ttcaaccaga gtaaatcctt cgaaacgttc caatacagta aaatgtgtga cagccgactt
    16681 cccatcttct cttatcgcct gcattttccg gttttccttc gaacggccga ttggcgcatt
    16741 gatggttcct ttttcgtgag gaatctctcc atggactaaa gcaacatatt tgcgcaaaga
    16801 ggtcttatct tttaattgct gagctaatga ttcgtgtgcc tggtcatttt tggcaaccat
    16861 tagcagtccg gaagtatctt tgtcgattcg gtgaacaata cctggacgaa taacgccgtt
    16921 gatcgtagaa agatccttga tatggtacat cagcgcattg accattgtgc catgcgtatg
    16981 acctgctgaa ggatgaacga ccattccttg aggtttgttt atcaccgcta catcttcatc
    17041 ttcgtaaacg atcgtcaacg ggatattttc tgggaccaca tctaatgttt ctggttcggg
    17101 gatcaagaca gtaatctcgt ctccttgttt tactttgtaa tttgctttga ctggctgtcc
    17161 attgacagaa accgctccat ttttcagcca cagctgaatt tgcgaacgac tgtaatcggt
    17221 gaataaatta gtcaatactt tatcgatcct accagattca tggtgaatcg tcgttttaag
    17281 ttctgtcatt tttttctttg atccttttca tctaaaataa tataaattaa catacaaatc
    17341 actccacata caagtgtcac atctgctacg ttaaaaatcg gaaagctcat aaaatctgtc
    17401 tggaacatat ctactacata gccaagacga atacggtcga taaaattgcc aatcgcacca
    17461 gctagaatca aactcaatcc aatcatgaac cagcgattac ctttttcacg atttttaatc
    17521 atcaatgtga gaatcacaac gactgcaatg agtgtcacta tagtaaagaa ccacatcttc
    17581 ccttctagca tactccatgc ggctccagtg tttcgaatat gattcagtga caaaatacct
    17641 ggtataagat ttttcgtctc tccaagcgct agattatcag tgatccacca tttgaccaac
    17701 tgatccactc ctactagcaa agcgctgaat atccaataaa taattaacaa aaaatcaatc
    17761 cttttctatt tgtttataaa tatcacgagg tcgtacgatc ccgagcagat taccatcggg
    17821 attcccatct ttagtgataa gtacggcttc caaagtccct tgctcacgaa acatcgcttc
    17881 aacctcgtaa atatgtgttt tagctggaac aaaatgataa tttgtgtact ttccgttttg
    17941 gatgatcaaa tccaacgctc gtttatttgt taaatcaacc ggaccttttt ggctttcttt
    18001 tgctagccaa aacccgatat tacgtaaagt aatcaatgct acaaattttc ctttattgta
    18061 taacggaaat tgtgaatacc ttttttctgc tactatcttt aacagttcaa gtaacggaag
    18121 atcttgttca aaacctgtta cacgttttgc aaatcgaggc agcacggttt caggctgaat
    18181 cagttcttgt tcgatcagct ggattcgctt gactgtccaa tcatttggct ctgcaatgac
    18241 aaaatcgact gcgatccgat catggacgat cgcattcctt aactgagcaa gctgtaaaag
    18301 atcatcttcg tattttctga tcattaattg ttttctttga gctaggcgtc tgacaagttc
    18361 ggtaaatccc atatttcgtg catttcccat ctcgtctctc atccattttt cgattcgatt
    18421 gaaactgatt aaaaaaagat cagagtttgc cataattttc ctccttgatc aaatatgccc
    18481 ggttttttat atagaaaaag aacgcaatca acacaataac tgcaaaaaaa ctgaaaaata
    18541 aattttgtag gaaaacggcc cccaacccag ttgctagaaa taaaaacgga tagatttgaa
    18601 gaatcaagta attcattgtg atcacataag caggctttgc ttctttcact gtataaacgg
    18661 ggctttttag ccaataaaag gtgacctgca tcgtaaaggg cacttccaga tcaagcgtta
    18721 tcttttgttc atgactgaga acactgcgtt tttcattgtt gattcttagc tgaatcgagc
    18781 tacccatacc gtaaaaaccg gtttttctta taaatgtcgt tttcatcata ccctcctatt
    18841 atgctttagt atagctcaaa atacaataaa gaaaaagaat gatcctagtg taaacaaaat
    18901 taattcccta tcgtctaaaa aaagaagctg tagtcaaaat acagctcctt cgatggtaga
    18961 ttataatcaa gcttaaaaca atcccaacac ttctccgtca tttgtcacgt ccatattcaa
    19021 tgcagcaggt tttttaggca aaccaggcat cgtcataaca tctccagtca aggctactaa
    19081 aaatcctgcc cctaattttg ggatgatttc gcggatcgtg atggtgaaat cttccggtgc
    19141 acctagcagt gaaggatcat cagaaaatga atactgtgtt tttgccatac aaatcggcag
    19201 tttgtcccat ccattcttcg taaactcttc tatttgtttt tgcgcttttt tgctgaaaac
    19261 aacaccattt cctccgtaaa ttttcttgac gattgtttct gtcttttctt gtatcgtttc
    19321 ttctaggcgg tataatggct tgtaattagc ttcttttcta tcaatcaatc gtactacagc
    19381 ttttgctaaa tcaacgccgc cttctgctcc atctgcccat acactcgctc tcttggcaag
    19441 aatcccctga ttttcacaca agtgctccaa cagtgtgagt tcattgtctg tatctgttac
    19501 gaattcattg attgcgacga tcacaggtag ctgatattgt tccatattac gtatatggcg
    19561 ctttaaattt gcaaaaccta ctttcaaagc atcaagattc tcatttttaa gttcatcttt
    19621 tttcattccg ccatgcattt tcaatgcacg gacagtggcg acaatcacca cagcatccgg
    19681 cgcttttttc aagttaggta ctttgatatc taaaaatttc tctccgccaa gatctgcacc
    19741 aaatcctgcc tctgtgacaa cgtaatctgc taatctcaat gctgtcttgg tagctaaaat
    19801 actattgcag ccatgagcga tattggcaaa cggtcccccg tggacaaaag ctggtgttcc
    19861 gtaaattgtt tgtacaagat tcggcttgat tgcatctttc agcagtaatg ccagagcgcc
    19921 ttctgctttc aagtctccag cagtcacagg ctgacggtca aatgtatagc cgacaacgat
    19981 ccttgcaaga cgagccttta aatcttcaag gtctgccgca agacacaaga tagccatgat
    20041 ttcgctggca actgtgatat caaacccatc ttctcttgga acaccttgga ttggcccacc
    20101 caagccaaca attactttac gcagttcacg atcattgaga tcgactaccc gtttccaaat
    20161 cactcggcgg gcatcgatgt tcaattcatt tccttgatga atatgattat ctaaaagagc
    20221 agataatgca ttattagctg ttgtgatagc atgcatgtca ccagtaaaat gcaagttgat
    20281 atcttccata ggcaatacct gtgcatatcc tccgcctgca gcaccacctt tgattcccat
    20341 cacaggacca agtgagggtt ctcgcaatgc aataacagat tttttattga ttttattgag
    20401 cgcgtctcct aaccctacag tgatcgttga ctttccttcg ccagctggtg ttggattgat
    20461 ggaagtaact aagatcaaat gcccctcagc gttatccttt gatcgattga ttccagaaaa
    20521 attgattttc gccttatatt tcccatacag ttccaaatcg tcttccttaa gatctaattt
    20581 actagccact tcaacaatcg gttctagctc tgtttcttgt gaaatttgca gatcattctt
    20641 catttgatta ccccttttca cgaactttat tcaacaaaat atcttctttg ttaagatatg
    20701 ccaattatac ctttttttat tcagaaaaaa aacataaaat tacaggtttt taatttattt
    20761 gacgtactta taaatactct ttataatgaa ggctatgatc agactttaga aaggtcgtga
    20821 gtgctttgac cacaggaatt gtaaaatggt ttgacaacaa aaaaggatac ggctttatca
    20881 gttatgatga cacagaagaa atttttgtac attttactgc gattgaagaa gagggattca
    20941 aaactttgga agaaaatcaa gtggtcgaat ttgagatcat cgaagggaat cgaggaacac
    21001 aagctgccca tgtcaagaaa ggcaacgttc agactaccga tactagtatg catgaataga
    21061 taaacaaata gagtgagacg ctaaacggga tgaaccgtac gtctcactct tttcttttcc
    21121 tgtaaaaatt cgttttttta ggttctgatt tttaaaatta gctttttctt tttttcgtat
    21181 aaggataagg aattgattct gatttgggtc cgtttttttc aaaatgatca aaaaggacta
    21241 cttgatcttt ttttcgcatc atgaaaacag atggggcttt ctcatcttgc aacatcattt
    21301 gcaccccagt attagttggc gtcaaagatc gaatctgtcc gacagatatt tctcggtctt
    21361 tccaaaaagc agcataacgt ataatcacag agtttgattt taaaataaaa gaacgccgca
    21421 aacctagaaa tagaaaaaca agaaaaatac ctaaaacaaa attgcttttc caataaggac
    21481 ctgtgttttc taaagaaaga atcagactaa aaaacagaat gatcaaggtc aatgaccaat
    21541 agatgatcgt atgtgccagt tcaggctgcc atcgaatatt ttttttcatt ctctaacctc
    21601 tcaccgattc agtaatgata taatcttacc ataacattag tttgaacaaa aaggagaatc
    21661 tctttttatg ttaaaagtat acattgatgc ctctacaaaa ggcaaccctg gtcctagtgg
    21721 aggtggaatc ttgattgtct atcaaggtaa acaagaacag ctatctatcc cgctttctat
    21781 agggacaaac catcaagcag agttcgaagt ctttttaaaa actttagaat ttctaaagaa
    21841 aaacgactta cagaatgaaa cgattttctg tttttcagac agcaaaacac tagtttctac
    21901 ggtcgacaaa aacgcaacga agaacgagct cttccttccc taccttcaaa gaatccaaga
    21961 acttctatcc gaattttctt tattgatcct gcaatggttg cccgaaaaca aaaataaagg
    22021 agcagataat cttgcacgtc aggctctcca taaacaatta aaagaaaaaa agactcttta
    22081 aattaaaaac tttaaggagt ctttttatag tttctcattt aatataaaag ataattgtga
    22141 tcaaacgtaa tttccaattg gtaatctccg attttttctt ctatttgact acgtaagatt
    22201 ctttctagtt ctgtatcaac aaactggaat ttctcaggga ccactacatc aaattgcagc
    22261 gtttttcctt gatgaattac tctaaaatca tgaatattaa gccctgtacc tgtttcaaga
    22321 agaatttgct ttaactgccg caagatatta tagtattttt cgttatcaac aggcattgga
    22381 tccaagtgac agacaagttc cacatccaaa gactttttaa agtctttttc tatcagatca
    22441 ataatcttat gagccacatt caaattcatt gattcatcta cttcgatgtg gactgatgca
    22501 aagcgtttat tgggtccata gttatgcacc aataaatcat gatagccaag tatatcatcg
    22561 caagcattta aatgctcttc catttcttca atctctgctg gcgttgggcg acttcctaat
    22621 agttcgtata caaaatttcg aagcatcgtc aatccactat aaaggatata taatgctaaa
    22681 gcaaaaccta tatatccatc aatacgccat ccagttaccc attcgattgc tgctgaaagc
    22741 aatacagcca gtgtagtaaa gacatcatta taactatctg ttgctgtagc tctcaatgtt
    22801 tcagaatcaa tggattgcgc aataacctga tacattttgc cttgtgcaaa tttcaaaaag
    22861 atggaaatga ttaggacaaa aaagacgatg ggtgttaaag agacattttc tggatggatg
    22921 attttttcag cagaagactc taaaaattga aatcctacat aagtaatgat gatcgaaaca
    22981 aataacccac tgatatattc gaagcgttcg tgtccataag gatgttcctg atctgctggc
    23041 tttgcagcaa ttttaaatcc gatcaaagtc aatatggatg aagcagtatc tgatagactg
    23101 ttcactgcat cagccataat ggagacactt cctgaaaaaa gcccgatcat tagcttagag
    23161 acaaacaaga tcagattcgt aaccaatcct aagatacccg ctagtactcc tttttttgtc
    23221 cgctgttgcg gtatttgttt ctttttttct tcttgtttct tagccaagtt ttttacccct
    23281 tctagtttgc ttgttgctca ggtaaaaaga tggagaggac gattgttttc ctctcctctc
    23341 ttcctttttc tatctatcgt gcgtcctatt actattaaaa gatggtcatt attctggttt
    23401 caacggtgtt gctgcaaata acgcttttgc tagtaatggt gttatgattc cagcacctat
    23461 tgcttctggt atcccattga ttgctgcaac ccctgctaag gttttaaata aaagagcaga
    23521 gtctacacca taggcatctg ccgttgcacc tgtataaaat aatcccatga atcctaacac
    23581 taagactgta ttgaccaatg aacccagtac ccctgcaaag gctgagctga tcgttattga
    23641 tttatttttt tgataaatcc agtggaaaac caagcctgct actaagccaa caagtactct
    23701 gggaactacc gagataatcg gattcgtgaa cactaatgta tcaaatggcg ttgttggcat
    23761 ggcataggca cgaacaattg tagcgattcc ccaaaacaag ccaatcacca tgccatcttt
    23821 tgggcctaat actactgcag caataattac tgtgatatgg ataattgtta aactaatgaa
    23881 tcctaatgga ataaacccca gccaaggaac catcgcttgt acgatgatga ttgctaacaa
    23941 gatcgcacgt aagactagtc gaaaggtctt atttctacta ttcatttttt cacccctttt
    24001 tatttatact aatcgtaaaa atttctgtca ctttcgtcaa ctaaagacca tccgaatccg
    24061 accatttgat tagatgcgcc atccaattga aaaaagtatg agggcaagac ttgtttgtct
    24121 tcgcccccat aatcatgttt cgttcaatgt ttgaactttt gtttttctgt caaacgtaaa
    24181 cgccctattg ttgcgcaacc cattctttcg cagctttaag tgcatctgga ataccagcag
    24241 gatttttccc gccagcttgt gccatgtctg gacgtccacc accgccaccg cctactttcg
    24301 gtgcgattgc tttaatcaag tcgccagctt ttagtccagc ttcatttgat tctttcgtca
    24361 ttgccgctag cagactgact tttccttctt gttcgtttgc taaaacaaga atatctgaaa
    24421 tatttttttg tttccattgg tcagctaatt gacgcagctg attcatatct ttcactttta
    24481 cttgtgctgc aatgattgtt tttccattga tcgtttctgg atctttgaat acatcaccag
    24541 cttgttgatt cgctaacttg ctagctaatt gttcattttc cttttgtagc tctcttactt
    24601 gctgttgcaa ttgttccgct tttgaaacaa cttctttcaa ttgaggtgat ttaacaattg
    24661 ttgctacttg ttttagttgt ttttcttctt cttcaagtag ttcgtacgct tctttacttg
    24721 ttacagcctc gattcgtcgc acgcctgcac caattcctga ttcagaaacg attttgaaga
    24781 ttccgatgtt ttctgtattt gctacatgtg tgcctccgca cagttcgatt gaccagccgc
    24841 caatatttac tactcgaacg tcatgtccgt atttttcacc aaacaaagcc attgcaccca
    24901 tgtttttggc tgtatcgata tctgtttcaa ttgtttcaac tgggatagct tcccagattt
    24961 tttcgttgac gattctttcc atttcagcta attcatctga agtgatttga ccaaagtggg
    25021 taaagtcgaa tcgtaaatgt ccaggagcga ctaaagaacc agcttgattt gcatgctcgc
    25081 caagtacttc tttcaatgcc ttgtgcagta gatgggttgc tgtatggttt ttgatgatcc
    25141 gattgcgcat cgattgatcg atcaatagat gataggtttg tccttcactc attggtgctt
    25201 ttacttctac ggtgtgcata aattgaccat taggcgcttt ttgaacatct gtaacaactg
    25261 ctacgatttc gcctacttca ttcatgatcc atccttgatc cgctacttgc ccacccattt
    25321 ctgcatagaa aggtgtttga tcaaagatca tttgagcttc accttctgcg acttcattca
    25381 aaagagactc acctttaacg atgaccaata attgactatc tgattcttgt tctgtgtatc
    25441 caatgaattt actttctact ttgatatctg tcaataaagc agattgtacc cccattgatt
    25501 tttcagtact gcgggcagag cgagcacgtt cgcgttgtgc atccatttct ttttcaaaac
    25561 cagcatgatc aactttcagt ccttcatctt ctgccacctc ttccgttaat tctaccggga
    25621 agccatatgt gtcgtatagt ttgaagatgt cttttcctgc tagagtcgtc ccgttttcgt
    25681 tttttacttc ttggatcact tgatttaaga tttccaaacc ttcattgatt gtttcattga
    25741 aacgttcttc ttctgtacgg ataacttttt caataaattc tttttgttga agtacttcag
    25801 gataataact taccatgatt tccccaacaa cagggactaa acgatataag aatgcttgtt
    25861 caatacctaa ttttttccca tgcatgacag cacgacgaag caaacggcgt aagacatacc
    25921 ctctcccttc attcgatggc aatgcaccgt cgccaatagc aaatgaaagg gcgcgaatat
    25981 gatctgcgat tactttgtaa gaaatatctg ttactgggtt ttcaccatat tttacttgtt
    26041 tgcttagctt ttctacggcg tggataatag gcataaacaa gtctgtctca aagttcgttg
    26101 acgcatcttg aacgattgag accatgcgtt ccaatcccat ccccgtatca atgtttttat
    26161 gaggaagtgg ttcaaatgtg ttatcaggct tatggttgaa ttctgaaaat actaagttcc
    26221 agatttctaa ataacgttca ttttctcctc ctggatagtt ttcaggatcg tcttctgcta
    26281 aatcgttata agcttctcca cggtcataga aaatttccgt atccggacca catgggcctg
    26341 caccgatatc ccagaagttg tcttctacat caataatgtg atctagtggc aggccaactt
    26401 cttcatgcca gatgcgtttt gcttctgtat cttttggata aactgttaca taaagttttt
    26461 ctggatcgaa tgccatccat tttggatcag tcaaaaattc ccatgcccag tggatggctt
    26521 catttttgaa atagtcacca atcgaaaaat tacccagcat ctcaaacatc gtatggtgcc
    26581 gtgctgtttt accgacattt tcgatatcat ttgttcgaat agatttttgg gcatttgtga
    26641 ttcttggatt ttctggcaca actgaaccat caaaatattt tttcaatgtt gcaacaccag
    26701 agttgatcca caataaggtt ggatcgttca cggggatcaa agacgcacta ggttcaatcg
    26761 tatgtccttt agattggaag aaatcaagaa acatttgtcg aacttgtgca ctacttaatt
    26821 gtttcatgtc attacctgct ttctataaat taaatacaaa aaaacaccat cacagccaag
    26881 gacgaaaact cgcggtacca ccttgattgc aatgatgtgt aatcattacc tcttaaactc
    26941 gataacgctg agctgcgtcg gtatttctac caaaatagaa aaagggagca atttgattag
    27001 tgactattct tttttcagcc aagaaagaat tttctgtgag tcttaaaaaa caaatcatat
    27061 ccttttcgta cttaattagt atacgcattc tattgatcac tgtcaatatc tcaattttta
    27121 ttttcatttc cttttggtca tactccacga tttttggtac gataagaata aaggaatcaa
    27181 tacatctctg ctattcatga caaggaggaa tatgatgaaa aaaatagttg gagcggatga
    27241 atggatacgc gcagctagct gctttttgag atatcaagtt tttgtattag aacaagggat
    27301 tcctgcagaa ctagaattcg acggaaaaga tatcccgcaa caaacctatt atctatttct
    27361 tgatcaaaaa agaccagtag ctactttgcg ttatgaaaag aaaacaccta cttgcttcaa
    27421 gccggaacga ttttgcgtag caaaagaata ccgaggaaaa gggttaggcg gtcagttgct
    27481 tgaagctgct gaaaaacggg cggtttcaga aggttgtcag actagttatg tagtagctga
    27541 aatgacggct gtagattttt atgaaaaaca agggtatcat acttgcacag aaccttttat
    27601 tgaagatgaa gtactttgta tcggaatgaa aaaaagtcta gcttaaaaac aagagattgt
    27661 gacacgcctt ttgtcacaac ctcttgtttt ttatttatgt gaatttttct tatttttacg
    27721 agcagtacgg ctttgctgtc tgcgttcgat tttgcgttct tttttatttt tttctgcaat
    27781 tgcccaatcg atttttttct tataaccagg cttgatcttt ttctttttct tcttcacaag
    27841 accaatcagc gtgggatcta atacatcttt tgatttttct cgtttggcac gtctattacg
    27901 atcgtatgtt ttgacgattt ccccgttttt gatttctttt ggttcaaatg agacgcccaa
    27961 tttttctatc gcatcaatgg cttgctcatc atttggcgaa tataacgtaa tggccgttcc
    28021 ttttaagcca ttacgtccag tacgtccgac acgatggata aagaagtcta gatcttctgg
    28081 aatttctgca ttaataacat gagagacacc ttcgatatcg atccctctag cagctaaatc
    28141 agtcgctaca acatattgat actccagatt ctgtacttgt cgcatcactc gttttctctc
    28201 tcttggggtg atgtctccat gaatctttgc tactttcaag ccttggtttt tcaagtaatc
    28261 ggcaatttca tccacatgct gttttgtatt ggcaaatacg attgctaaat aaggatgacc
    28321 aattgtcagc agttgataaa tcatttgatt ctcatcttta ccttttgttg aaatcagcca
    28381 attttcaatg tcttcagaaa taactgcttt tggtttgatt tcttcaatga ttggattttc
    28441 catgtatttt cgtaagaatg gacgaagttt ttctggaata gtggcagaaa aaactaaaaa
    28501 ctgcagcttt tctggtaatc gacttgcaat ttggtctact tcatttaaaa aacccatatc
    28561 caatgtcata tccgcttcat cgacaacaaa tgcaaatgcg gtgtgaatct tcagcgcttg
    28621 ttcattcatc atgtctaata ttctaccagg cgtgcctact acaacctgtg gctgttggtg
    28681 atgcagacga tttaattgac gctgtttgtc tgtaccgccg acaaaattcg agacacggat
    28741 ctccggtgta gaaaatttag cgatttgtgt agctgcttca tagatctgtg cagctagttc
    28801 cctacttgga gctgtaataa caacttgtac ttcctctttt tctgcattga ttttttcaaa
    28861 caaaggcaac aaaaacgtat gtgtttttcc tgtaccagtt tgagattgcc caatgatatt
    28921 ttttcctttt gcaattaaag gaatcaaacg ttcctggacc tctgtgggct gtttgaagcc
    28981 ctttgcggtt aacgcttcat tgataaacgg tttgaagcgg aatatatcaa aattattcat
    29041 tcgtgcacct cttctcttta aattatcacg actttggcat tataacacag acaaagcgtt
    29101 tttgctcagc taatttcgag aatcatcatc ctttgtttgg ccaatcgtgc taacagtata
    29161 tgtgtgacta gctttttcat aaaacagaat ccgttttttt ctcaaaaatg atagcaagat
    29221 atttaaaata aaaatacctt gtagtaacaa gacaccgaga gaaatgaata ctgcactcat
    29281 ctgcaacacc gtattcgttg tcgaattcat gatttgctga agcaactgcc ccaaccagct
    29341 taaaccataa aaaagaaagt acagataccc gtagcggatc atcaatgctt ttaaagaaat
    29401 tcgttcgcgg tcttcttcca ccaaacggat acgcacaatt tttttgccaa gtgtccatcc
    29461 attggttata aacggtacaa tcataaagta tacccctact tctaataaaa gccaccagat
    29521 atgagaagta actgcccctg aatcgaacgc aattttaaag aaaatcgccg ctaccaaatt
    29581 aataagagat aaaaatgtcc agtcgatcaa ataagcgacc aagcgtcgag tcaatgagac
    29641 agtctttcct ttttcataag aaagttcatc cattcgtctt cttgtaggaa gcataaatgt
    29701 aaataacggc gtaacgacaa aaccaatgat tccacccaat gtatttgtca ctaaatcatt
    29761 cacatcaaat aagcggtatg gtcttgggta gataaaataa agtccactta gctgcgtcaa
    29821 ttcaaaaaat agagaaaggc agaaactagc aatcatcgtt ttcataaaag aaagccggaa
    29881 ataatatctt aaataaatgc caaacggtat gagtaataaa acattaaaag ctggttccaa
    29941 aaatacactc tgcttcattg ccggcaggta ggtactaggt tcagacaaga ccaataccgt
    30001 gttgtctctg aaatgttgcc atgaagcaaa taaatgcagc tccatctttg gccctgtcat
    30061 ttgggcaacc gtctcctttg ccggaagtgg taagatcacc aaaaaatacg cacacaataa
    30121 ataaaaaacg aaagaataca aaataactgc ccttgttact agaaatgttc catactttcg
    30181 atattcataa ataaaaaaaa cagatgagat cgctaaggct aaaaatggaa aaacaatcag
    30241 cgcaaaacga atcggttcga tataacttgc catatgactc ctccttgatt ctttgtatct
    30301 ctcttatctt agcagtcctt tgctgtttca agcaagtaac gaggctatta tttctcattt
    30361 ctttcaaagt aaagtaagat aaacacaaag ggggtgtcga gatgctgaat atgtactttg
    30421 tttttggcgt acctattttt ttactgttcc tttatgctac gatcgcttat gtaagaaaaa
    30481 gaactacaat tcattattta ggatttattc tcctgattat ttcgggtttc atgctagtct
    30541 tcaatcttca gacttggcag caagcacttc ttgaaatgga taaaatgact ccacatgctt
    30601 tgtctaaagt gcttggctac cctgtctatc tgatttggct tcccattttc atttctggtt
    30661 gcttggtttt attaaatatt tatcgcggcg tacgccgaat agttcagtta cgcaaatcaa
    30721 agtaaaagac agaaatcggt tgagcccgat ttctgtcttt tactttgcta tttcatcgta
    30781 gacaaaacga catttacgtg atcaaaatta acgagtactt ttgacatttc ttccacgtac
    30841 tcctcattta aataaaatat tttgtttggc agagaagtgc agttctaata aaattcgaat
    30901 gttttagcta tctacgtttt aaatagagat aaatcgggat accagcaagt gtcaatacta
    30961 tccccatcga agctaaaaag gtctgagtga taagcgtgct tgatacgata aaagtccctc
    31021 caatgattgc tactagtgga ataaacggat acattggaac tttgtatggt cgaaacaaat
    31081 caggttcttt tttacgaaga agaatgacac caacaaagac catcgtataa aaaatccaaa
    31141 tgacaaagat cagcatatcc gttagtgtat caaaaccacc gatcatcatc atgccaatag
    31201 cgatgactag ctctaagata cctgcggcta caggaacttt agttttatca tgaagtttag
    31261 caaagagctt gctaaaaggc aggttgtttt cttttgccat aacataaggg agacgcattc
    31321 cagtaagggt atagccattg atggttccat aaacagaaat caagatacca actgtgacca
    31381 gtcttccacc gaagccgcca aaaatcattt ttgcaacgtc acttgccgca ttgctgtttc
    31441 ctgcgactcc gtcgatagat gcggttctta agaacaccgc atttactaat aaataaacga
    31501 tcatgattcc gatgattcct aaagaaattg cttttggcaa atctttcgcc ggctttttca
    31561 attctcccga aatatttcct acgtgtatcc aaccgtcgta tgcgaacatc gttgctagca
    31621 agcccgctcc caaggctgag aaaaatgaaa gattttctcc tgcttggatc ggaaacagtt
    31681 ggaagtctac tccttcttgt cggaacaaac cgaaaatgac gatcacaaac aaaggaataa
    31741 gcttgcagac aagcgtaatc gactgaaaag cccctccagc tttagaacct aaaaaattga
    31801 tcaacaggat cgatacagca gctgtaacag ctacgggaac aatcattgac tgggaaagac
    31861 caaatagatt gacgaactgt gtaccgaaaa tgatcgacaa ggctgctaca tttgctggaa
    31921 aataaatcac gacttgcgcc cagcccaata aaaaagctgc tgtatttccg taaatccgtt
    31981 cgatatattt aatcatccct cctgtttctg gaatagcagc tgccagttca gctcctgtta
    32041 gtcctgcaca gacagaaatc ataccaccga gaaaccaaga aaacatatgc aaactggcgg
    32101 atcctgtaac ttctgctacg cttgcggctt taaaaaatac ccctgcaccg atcactgtgc
    32161 ccataacagt cgataaagca gtaaaaaatc ccatcgtacg ttttaattca ttttctattt
    32221 ttacttcttt tactggcgtg gcttgatatt cttccatccc ttgtcccctt tcataccata
    32281 gccaatccca aaagtataaa aaactttttg gatgtggttc ttgttttata ccgtctctac
    32341 caatttttct ttcagttttt ccaacatatc ctttgtcatc acatctaagt cgtatttcgg
    32401 cgaaaagccc cattcctctt gcgcacaaga aacatctagt gaatttggcc aagattctgc
    32461 tattgactga cgtacaggat ccacatcata atccatttca aaatctggta tgattttttg
    32521 aatagatgct ttgatctctt ccgggtcaaa agacatcgcc gatatattga aggcatttcg
    32581 atgaaccaat cgatcaggat ctgcattcat gagttttata atggcatcga tcgcatctgg
    32641 catatacatc atatccattg atgtaccttg atcaatgaaa ctagtgtact ttccttcttt
    32701 gattgctgaa taataaatat cgactgcgta gtctgtcgta ccgcctcctg gcaaggttgc
    32761 ataagaaatc aatccaggga aacggacacc acgagtatct acgccatatt tcacataata
    32821 ataatcacat agtagttctc cagctacctt cgttacccca tacatggttg taggacgctg
    32881 aagagtatct tgtggggtat gatcttttgg agtatttgga ccaaacgcgc cgattgaact
    32941 tggcgtaaaa aatttcagct ggaattctct agcgacttcc aatgcattga ccagcccagt
    33001 cacgttcaaa ttccaggcta attgtggttt tgcttctgca acagctgaca aaagagcagc
    33061 caaatgaatc aatgtgtcca cttgatattg ttctgctatg ctgcgcattt tttcgtaatc
    33121 cattacatct aggatctcaa agatatcgtt tttctcaatg acttcaggca tgcgtatatc
    33181 tgtcgcaatg acattttcac tgcctagttc ttgacgtagc cgtgtgatca actctgaacc
    33241 aatttgtcct aaacatcctg tcaccaatac ttttttcatg atgatcctcc ctgatctttt
    33301 ttactactat cttcagatga ttaacaatat atgcgtgaac atgttctcta attttaaata
    33361 gacgaaaaaa ctaaatttag ttaaataatt tccatttctt ttcctatttt ttgataaact
    33421 gctactgctt catcaagcat ttccctcgta tgttccgcag tcggcatatt tctgatcctg
    33481 cctgtcccaa gaggcacagt tggataaacg attggtttag cataaatccc atgttcgatc
    33541 agcttcttag agaaagtctg agtcactttt tcgtctccca aaatgactgg agtaataggt
    33601 gtttcagaca taccgatgtt atagcctact tttttcaatt cttctttaaa ataattagca
    33661 ttttcccaaa ctttttcgac aagttccgga tgttcttgca tcaaagtaat cgaagccaat
    33721 gcagctgcag ctgcacctgg agttagcgaa gtagaaaata ggaatggccg acttctagct
    33781 ttcaaccagt cgattagtgt tttcgatcca gctacataac ccccaacaac acctatagct
    33841 ttcgataacg tgcccatttg catatcgatt ttgtcgctta atccaaaatg cttgacggtt
    33901 cctgcacctt ttccagttac gcctgagccg tgcgcatcat cgacgtaggt gatcagtcca
    33961 tatttttcaa caatcggaat aatttccggc aatcgtgcaa tgtctccatc catggagaaa
    34021 acaccatctg tgatatacat gattttttta tatttcttac tttctatcgc ttcctttgct
    34081 tttttctcaa ggtctttcat atcttgatgt ttaattcgga tgatttttgc tccggataag
    34141 cggcaaccgt caataataga tgcatggttt aattcatccg ataagatagc atcttctttt
    34201 gtcattactg cagagatagc gcccatattg cagttgaatc cagattgaaa ggcgattgct
    34261 gcttctgtcc ctttgaattc tgcgattttt tcttctaatt gctgatggat tttcagcgaa
    34321 ccattgatcg ttcggactgc tcctgctcct acaccataag tttcggttgc ttcaatacat
    34381 gcttttttca attcttcgcg attggcaaat ccaagataat tgttggatgc tagattgatc
    34441 atcttttttc cgtctacgat gatacaagca ccattttcac tttctagtat gtcgatcgta
    34501 ttgtataacc ccttttcttt cagttgatcc aattctttct ctaaatacat tgttagttct
    34561 ttggaagaca ttgtctccac ctccatattc atctgtcttg cagaacatac caaacatata
    34621 tcgtgtcatt catctacaaa gcaagtatgc tatatttttg ataacgctta cactgattat
    34681 gagaattatt taaccacatt ttttatttac agtatctttt tactttcaaa tgacttatat
    34741 agtatgaaca attacctttt agataatgat ctattttact ctacctgggc agataaatac
    34801 aacacaacca ttgatagaag ccctttcgtt aaaatcaatt gtattctgaa tatttaattt
    34861 atttattgca tagaccaata aaaatacata aaatttccat cttcgtttat cagcaatttt
    34921 taattttact ctctgaatat ttcttaaaag ttttacttgt tgaattcgaa aaaaggactg
    34981 tgacaaaagt cttgtcacag tcctattctt ccgaata
//
LOCUS       DAZKMK010000017        34734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000017 DAZKMK010000000
VERSION     DAZKMK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.017"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8V1_000894"
     CDS             212..604
                     /locus_tag="ACG8V1_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0329008.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8V1_000895"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8V1_000895"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0329009.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8V1_000896"
     CDS             2698..2943
                     /locus_tag="ACG8V1_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329010.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8V1_000897"
     CDS             3165..4775
                     /locus_tag="ACG8V1_000897"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0329011.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8V1_000898"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8V1_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329012.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8V1_000899"
     CDS             5067..5936
                     /locus_tag="ACG8V1_000899"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0329013.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8V1_000900"
     CDS             6101..7372
                     /locus_tag="ACG8V1_000900"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0329014.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8V1_000901"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8V1_000901"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0329015.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8V1_000902"
     CDS             8763..9026
                     /locus_tag="ACG8V1_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0329016.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8V1_000903"
     CDS             9471..9920
                     /locus_tag="ACG8V1_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0329017.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8V1_000904"
     CDS             9977..11395
                     /locus_tag="ACG8V1_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0329018.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8V1_000905"
     CDS             11395..12255
                     /locus_tag="ACG8V1_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0329019.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13199
                     /locus_tag="ACG8V1_000906"
     CDS             12429..13199
                     /locus_tag="ACG8V1_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0329020.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEETEKRKIADTASSLIRDDMVLFLD
                     SSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSKA
                     YNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAHK
                     FMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13346..13711
                     /locus_tag="ACG8V1_000907"
     CDS             13346..13711
                     /locus_tag="ACG8V1_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0329021.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13859..15067)
                     /locus_tag="ACG8V1_000908"
     CDS             complement(13859..15067)
                     /locus_tag="ACG8V1_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0329022.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15086..15364)
                     /locus_tag="ACG8V1_000909"
     CDS             complement(15086..15364)
                     /locus_tag="ACG8V1_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329023.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15557..16213
                     /locus_tag="ACG8V1_000910"
     CDS             15557..16213
                     /locus_tag="ACG8V1_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0329024.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16655..17452)
                     /locus_tag="ACG8V1_000911"
     CDS             complement(16655..17452)
                     /locus_tag="ACG8V1_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0329025.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17449..18633)
                     /locus_tag="ACG8V1_000912"
     CDS             complement(17449..18633)
                     /locus_tag="ACG8V1_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0329026.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18658..19905)
                     /locus_tag="ACG8V1_000913"
     CDS             complement(18658..19905)
                     /locus_tag="ACG8V1_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0329027.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20120..21865
                     /gene="dnaX"
                     /locus_tag="ACG8V1_000914"
     CDS             20120..21865
                     /gene="dnaX"
                     /locus_tag="ACG8V1_000914"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0329028.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21885..22199
                     /locus_tag="ACG8V1_000915"
     CDS             21885..22199
                     /locus_tag="ACG8V1_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0329029.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22224..22820
                     /gene="recR"
                     /locus_tag="ACG8V1_000916"
     CDS             22224..22820
                     /gene="recR"
                     /locus_tag="ACG8V1_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0329030.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22884..23528
                     /gene="tmk"
                     /locus_tag="ACG8V1_000917"
     CDS             22884..23528
                     /gene="tmk"
                     /locus_tag="ACG8V1_000917"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0329031.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23543..23872
                     /locus_tag="ACG8V1_000918"
     CDS             23543..23872
                     /locus_tag="ACG8V1_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0329032.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23886..24824
                     /gene="holB"
                     /locus_tag="ACG8V1_000919"
     CDS             23886..24824
                     /gene="holB"
                     /locus_tag="ACG8V1_000919"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0329033.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24860..25684
                     /locus_tag="ACG8V1_000920"
     CDS             24860..25684
                     /locus_tag="ACG8V1_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0329034.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25677..26024
                     /locus_tag="ACG8V1_000921"
     CDS             25677..26024
                     /locus_tag="ACG8V1_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0329035.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26093..26965
                     /gene="rsmI"
                     /locus_tag="ACG8V1_000922"
     CDS             26093..26965
                     /gene="rsmI"
                     /locus_tag="ACG8V1_000922"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0329036.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27074..28195
                     /gene="dinB"
                     /locus_tag="ACG8V1_000923"
     CDS             27074..28195
                     /gene="dinB"
                     /locus_tag="ACG8V1_000923"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0329037.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28249..28851)
                     /gene="nrdG"
                     /locus_tag="ACG8V1_000924"
     CDS             complement(28249..28851)
                     /gene="nrdG"
                     /locus_tag="ACG8V1_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0329038.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29166..31355)
                     /gene="nrdD"
                     /locus_tag="ACG8V1_000925"
     CDS             complement(29166..31355)
                     /gene="nrdD"
                     /locus_tag="ACG8V1_000925"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0329039.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31681..32448
                     /locus_tag="ACG8V1_000926"
     CDS             31681..32448
                     /locus_tag="ACG8V1_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329040.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32438..34480
                     /locus_tag="ACG8V1_000927"
     CDS             32438..34480
                     /locus_tag="ACG8V1_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0329041.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11173 a         6145 c         7282 g        10134 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaaacaga aaaacgtaag attgcagata cagcttcttc tttgattcga gatgatatgg
    12721 tcttattctt ggattccagt acaacctctt tacacttagt cagaaaccta gcaagatttg
    12781 atggtttgca gatcattacg aatggcatcg tcacagctgc tatgttgagt gaatatacaa
    12841 cggcaaaagt atctgtattg ggaggggcaa ttgttccgaa acgagcaaca atcaacggtt
    12901 caaaagccta taacgatgca ttgtcatatt atgcggattt agcgataatg agttgtcgcg
    12961 ggctagattt cagccaagga gtaacagaaa ctcatgaagg agaggcaatg gtcaaaaaag
    13021 cttttagaaa acaatcagac cggcttgccg ttttagttac acagcaaaaa attgcacata
    13081 aattcatgca tcaagccatt gcttttcatg aaatcgatta tctggtgaca agtaaagaac
    13141 tgacaaaaga acagtatgcg aaagtaaagg aacaccatat cgaatgtctg tacggataaa
    13201 ttattacgtg aatgttctaa gtggcttcct catttttaga taggacctta tctttgctcg
    13261 cttctctcag ccgatggtat aataatgaaa aggttatctt gttagataat cttacttttt
    13321 tatccaggag aaaggagtgc agaaaatgaa acatgtgaag gcgttggtag tgaaggcaat
    13381 catgatctgg gcgattctgt gggtcgtgct gacaggttta tacggtgtta gctttatgga
    13441 ttcaacgatt gttggggtaa ttattgtagt catgatctat gtgctagggg atctgttgat
    13501 cttacgtaaa gtagggaaca tcgcagcgac gatcgcagat gctggttcag ctgcagttat
    13561 cttgtggctc tatctatatt ttatgaatga tacagtcgat atttggatga aagtattgat
    13621 tcctgctcta ttgatcggtg tggcagaatg gttcttccac aagtggttgc taagtcaagg
    13681 catcgtacca gatgaacgga aaatggaata aatcaacagt taaactattc ttatcgataa
    13741 atgaaagctt taaaaataaa tagatgttta attaagaata atcataaact atttttactc
    13801 aaaaaaccgc tcagattatt ttcagatctg aacggttttt tttatctatt catcaagatc
    13861 aaatttcatt tttcaatgat tcaatcaatt cgccctctgc ttcatgacta tgttttcggc
    13921 cattaacaag atagagtgct gtacatatca atggaatgac tccggcaatc acatacaatc
    13981 ctctaaatga agtaagagat tttagcatcc ccatcaaata aggaccaacg cctaagccta
    14041 ggtctaaacc aataaagtag gtagataaag caatactgat tcgatgttcg cggacgattt
    14101 ttaaacaaac agcctgtcca tttgacataa acgtaccata acctaggccg actaatgctc
    14161 cagagataag caatgtcaca ctattcgtcg ttacactcag taaaaataat cctgctgtta
    14221 agaataaata gctaggatac atcacgtatt gttctccttt gacatcgaaa atccttccag
    14281 tcaatggacg tgtaaatgtg atgaccaaag catatacgac gaagaaaaat gacccagcag
    14341 caactaagtg gattactttt gtataagaag ataaaaatga taaaacactt gaataagaaa
    14401 gacccatcaa aaaggcaatt cctgtaatga aaagtgcttt cttttcaata aaactattga
    14461 tattccatga gcgaagagat attttttgtt cttcagataa tggaatattt tttacaggaa
    14521 agacaaggca ggcaatcgta acaaagaaga ccagtacaat tgaaaaccag ataatgaaac
    14581 gaaaatcagt catattcagc aaaatcattc ctaaaaatgg cccaacagcg gcagctaaac
    14641 tagtacttaa tccataataa ttgatgcctt cccctttttt agatgcaggg atatatgcag
    14701 taacgatagc atttgtagct gtagaaactg taccgtagcc gaatccattc aagaatcgaa
    14761 ctaaatacaa tgccccgata gttggcgtat aaagatatgc taatgttgaa aggagataga
    14821 agatcgcgcc gcctcttaag gtaaacttcc gaccaaatag ttctaactgt ttccccatga
    14881 ataagcgtgc cagcaatgtt ccgatgatat agatcccaga agcaaaacct gcttggctca
    14941 agctggcgtg taaggtgtct tgcgcaatga ctgccatgat gaccatcaac agataataaa
    15001 tcaggtagac aataaaattg atcaatgtga ttccaataaa atatttatta aataatttct
    15061 tttccatgta tgttatacat tccccttaag ctgaatatgc ttttaatttt tgtatttgaa
    15121 cttctacatc gtgataattg gatggatagc gatcaacaag ttggcgaata aataaatctc
    15181 ttgtagacat aaacagattt aaaaattctt cgcattcgtt tgttggattc tcatccactg
    15241 ctttgatcaa ttcgatcgag ttttgatacg catcatttaa aaagactaaa gaagctttca
    15301 tatcaaatct tgtttgatat gctgtttcta atcgattaat gattgtgttc atccagtaat
    15361 tcatgttttt tccctctatt cttaaaaaat tttacttctg cagtataacg aataaaaaaa
    15421 aggaaaaaaa tcgcataagt gatggaaaaa ggtacttaac aaatgttaat atcattagta
    15481 aaataccttt ggactttaaa aagttttaaa gtactgtatg atctttttag atgaaagttt
    15541 ggatggggga gcatcattgg atagtagagt cttaaaggaa tatttggata aaaatgattt
    15601 tccgattata cgcaaaggat atcataaata tttgacattc gaagggatgg aagattccca
    15661 tacgtatatc ttgaaagagg gaacggtaaa agcaagtgtt atttcaaatg atgggagaga
    15721 atttaacctt cgctatatca aagatcttga aatcgtctct cttttaaaag acgaatattc
    15781 acaattcatc gattcgccgt ataatattcg agttgaatca gaatatgcag agttttatcg
    15841 aatcaatcgt gtgcgctttt ggcaaaatat caaccaagac ccaaagctac aacactatgt
    15901 aaaagagtat taccgctatc gtctgcttta ttcgatgaaa aaaatgcagc aaatgctatt
    15961 aaacggaaag ctgggagctg tttgtacgca actttacgaa ttatatgatt tattcggtgt
    16021 gcggacagaa gaagggatgc tgatcgattt ttcgatcacg aatgaagaaa tcgccaaatt
    16081 ttgtggcatc tcaactgcta gcagtgtaaa cagaatttta aaacaactga aaaaagatgg
    16141 tattatcacc gttaaagacc atcatatcgt tattttgaat atggacttgt tagaagacaa
    16201 tattatcttt tgatgaacaa agaggttgtg aaatcagcgt tttgacctga gtattagaga
    16261 aacaaacgga aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    16321 ctgattgaac accgtttacc aagaggttgt gagcctgccg tttaactctg agtcacaagg
    16381 aacaatttca aaaaacagct tttcatgttt gatgaatatt gtgacttgtg accgaggagt
    16441 tggtaggaga acaccgttta ccaagaggtt gtgaaaaagc tgttctgcat caagtaataa
    16501 gaagagccaa gtaaaaacag tctcttatgt ttttaacttg gctggactta tcaccgtaga
    16561 tgtaagcttt tgaacgccgt ttatttgcaa caaaaggggc tgtgacacga tttttgtcac
    16621 agcccttttt ttgcgaacat tcatttagca atttttagaa acttctgccg ccaccgccaa
    16681 aagtcccgcc accgctagaa tgggtagtac ttccgccacc gccgcttcca ggaggtgggc
    16741 ttttggggat tcttcttgtc gtgacaaaag aatttgtcag acgatcggtt ttttcagtta
    16801 atttgatact ggatttttct cggaatggat atttgtagat gcctgatttc aattgataac
    16861 gcgaaacagt cacaaaataa aagacaagac taaggataag agccaaaaca acagcaatga
    16921 tgatttctac tgtcgtcaaa accttatagc gtgtaatttt gccagtttct tcgtctacac
    16981 gataatgtcc tcctggaact ccgtcttcca catatgttgc tgatttagac aggtaagctt
    17041 ttgcagcatt gtaataggca gcatttgtca tctgatcata aacatcatct aagatcgaat
    17101 cgattctgct atcagtcaaa taatcgatca tattaccaga agtcgaaatg tagatttcac
    17161 gctgtcccat atctaatagc aatactgagc cattttgatc atttccgact gcgtctctta
    17221 agtaattatc agcaaagtct cttggttctt ctgaattaga tgtagtcgtt acgatcaaga
    17281 ctcgtccttt gattttttca ttcagctttt ctgcctgttg ttccaaagat tggatttgat
    17341 ctgttgtgaa taacccagcc tcatcattta ccgtagctgt atctgcatga gtagccaccg
    17401 gccagccaac acttactagc aaaagaaaga ataaaaaaaa ttttttcatc atatcaagta
    17461 acctccgatc agaaagagga tcgctagccc agcgaaaatc ccaagagcgg tcagtcccaa
    17521 ttttttctgg ctgatcggca ggacaccgct gacctttcct gtttgtccat tcattgcgta
    17581 gtagtagact tttttacttg agtcattgct tcgataagta acaagccata ccggcagtaa
    17641 tacgtaatta tttacttcgt ttgtgattga agcagaagtt cgcacaccgg tcaatgttgt
    17701 atatccattt gcggtatctt ttaacagctt ctctgaatag tcttttaatt cagattgaat
    17761 ctgtgatttg attgcctcat attcgatatc tcttttttct gcttggaacc cggctaaata
    17821 ctggctttta aaatcaattg ctttatctaa cgggaaaggt tgcacgcctt cgaccatctt
    17881 ttgttgcgta ttttttgata aggcattttt tactagttct ttaaaagaaa gcttgcctgc
    17941 tcttgaaaca gcaaattgct tcgtttctgt gtattcgatg tcacctatcc gccatatgcg
    18001 gataactgta ccattcgcct gcatggcacc atctacttca gcatctgtag cccaataagg
    18061 gaaatacaca cctgtcaact tatcgatttg atccttattg aaaaaagctt tgggaacaaa
    18121 ccattttttc ttggtccaag cgagaaatct gttgattgct tcttctttct cgacagcaaa
    18181 aggaagtact ttatttggca agaattctcc gcttaaacgt cccgatagta cgacgggatt
    18241 atggcagtag taacaataag tcgcagcagt tgtcgcatca gtcacgatct ctgcaccaca
    18301 gttgggacaa agaaaaagat ctatcgctgc ggtattatct gcagattcgt ccgctgtatc
    18361 tgaaaaaccg cctgcctcgt caaccgcttt gcgctcggct tcattcatat cggcaagctg
    18421 ttcttgggct gtaaaagtaa aatcttcggt aggtgaaggt tcttctttgc tttcttcgcc
    18481 agccatacgc gcttcttttt gcttttgctc atattggctg acttcttctt ctgtatagat
    18541 attcaggcag tattcgcaat gaaatttttg gtctttggga tcaaaagtca acggaccgcc
    18601 gcaattagga catttatgtg taagaacatc catcagctgc acacctttct aaataaatta
    18661 attaacgggg attcctttaa acggtttacc acagtttgga cagaatttag ggatgccgtt
    18721 ggaaagatcc acgatctcat tgcattcact gcatcgcatt tgtagcttag gctgaacgtt
    18781 ttctacaggt ttggcagctc cgcagttgga acagaatttt ccggaatttt ctgtcccgca
    18841 aactggacat gtccaagtat ccgcatttcc ttgtgcagtt tgttgatttt gtttttctgc
    18901 ttgttgtttg atttgttctt ggttattttg agctgcttga gaaagataag aaccatttgc
    18961 attcattccc atacctacac ccataaagcc ggtcattgct cctgcttcat tttttccggc
    19021 tgcttccata ccacgtgcaa tggaaccttg cacgtagcct tcacgtacgc taggatcacc
    19081 tagcatcgcc ccttcgttac gcatattgat cagtttcaca gaatcatcgg tgtaagaaat
    19141 gctggctaca gccacagaaa caatctccat cccacgtaat tcacgccaag aatcatccaa
    19201 tgcagtatcc atgtatttgc ttaattccaa actcttagat ggtacataag aaatccgttg
    19261 accatctgca gacatctgat tgattgctga ttgtagcgct gttaggaatt cagctaaata
    19321 ctgctcattg atatcattga tttccacttg tgttttgttt ttagggattg cgtttgtata
    19381 aaataaaatc gggtcaacga catgaataga ataagtgcca tgtgcccgta agaataattc
    19441 tgcgttatag aaattgtcaa agtagttcaa cggcgcgctt gtaccgaatt tgattccctt
    19501 tatttcttgg agattgatat aaaaaacttg ttgtttctgc ggtgtgacgc cgccaaattt
    19561 gaatcggtca aaagtttcag caattgcttc tttcagtgtt ccattgaaca tggaaggagc
    19621 tgcgtcattt ttaatggtgt aataaccttc ctcagcggta taatcgatga tttttccacc
    19681 atcgaccaaa agcatcatca tgtttggata gacatggacg actgttccgt ctgttaaaac
    19741 atcttctgtt cctttacgat tggagccacg tttgtcatct ttgcggactt tgacaccttt
    19801 tgtcatgacc gttgtatcac tcatattgtc tggttcgatg acttctagcc attgatctgc
    19861 cagaccgccg ccaaccatac ttgttgctgc tttgataaga cccataaaaa attcctcctc
    19921 tgattcagct agtgccaaac aattattgtt tatttattat accataggaa gatgtgaaac
    19981 ttctcggact agagacggaa ttgaaatgga aaattgcgta ttgtttaaat acctgaaaaa
    20041 gccgagaagg atagtagttt ttttacctaa tatcacgtat aataagagat agtgaatttt
    20101 ttaaaaaagg gggatcctta tggcatatca agcactttat cgagtctggc gttcgcagcg
    20161 tttcgaagac atcgttggac aaaaagctgt gacacagaca ctaaaaaatg ctattgtttc
    20221 ccacaaaacc tcacatgcct acttgtttac aggacccaga ggaaccggaa agacgagtgc
    20281 agcaaagatt ttcgcaaaag caatcaactg tccgaacagt aaagacggtg agccatgcaa
    20341 cgaatgcgaa atgtgtcgat caattactgc tggtacgcaa gaagatgtga tcgaaatcga
    20401 tgctgccagc aataacggag tcgaagaaat ccgatttatc cgtgatcggg caaattatgc
    20461 gcctacaaag gcagcgtaca aagtgtacat tattgatgag gtacatatgc tttcaacagg
    20521 agctttcaat gccttgttga aaacattgga agagccaaaa gaaagtgtca tattcatctt
    20581 agcaacgacc gaacctcaca aaataccagc tacgatcatt tcacggactc agcgatttga
    20641 ttttaaacgg atcaatacgg cagatattgt cgaacatctt gaatttatct tgaaagaatc
    20701 gactatcagc tatgaaccag ctgcattgaa tgtgattgcc cgttctgcag aaggcgggat
    20761 gcgggatgca ttgagtattc ttgatcaggc aatctcgttt agtgaaggaa caattacttt
    20821 agatgatgcc atgcaagtga caggtagctt aactgacgaa atgatggaca gttacttgag
    20881 tgcttgcgtg aaaaaagaag taccagaagc attggaaaca ttgaaccaaa ttctcgcagc
    20941 cggcaaagaa tcacgtcgct ttctagagga tatgttacaa tactgccgtg acttgctgat
    21001 gtatcagcaa gcacctaacc tagttgagga acaatcgagt acgatcagaa gtacatttac
    21061 agaattggca caaactgttt cagcggatca gctgttttca atgataaaga ttttgagcga
    21121 tactcaaaat gaaattcgtt ttaccaatag tgccgctatt tatttggaag tcgctacagt
    21181 caaacttgca cagctaccgt ctgcttctgt tccttcagca gatgtagcat ttgcagatga
    21241 tcagatagta aaacagctac aacaagagat taaggaacta agaaacgaaa tcaaggaact
    21301 acgcagtaat acaggaattg agaatacaaa agtacagccg gtacagcaaa caaatcataa
    21361 aaaacagaca agtacctatc gtgtaccgac ggaacgagta tataaagttc tgcgggaagc
    21421 gacaagacag catctgaacc aagtaaaaga agtatgggaa gacttgctga tgagtctatc
    21481 tgtcactcag cgagcaatgc ttaaagcaag cgaaccagtt gcagctggac ctacaggact
    21541 agtgattgcg tttgattatg agattgtttg tcaaagagca gcaaatgacg aagaattgca
    21601 gctttcggta cacaacaact taagccgaat gatcaaagat tatgcgcctg atcctgttta
    21661 tattacaaga gaaagctggc cagaacttcg aagaaattat ttgacgcagg cacaagaaga
    21721 gggaacagaa aactcttttg cttctgaaga ctcagaagga gaaatcgagt tgcttcctca
    21781 agaacaagaa gtaccagaag aaacacttgt agtaaaagca gaggaactgt ttggctccga
    21841 agctgttaca gtggtagatg attaataaaa aggagtagat aattatgatg cgtggaatgg
    21901 gcaatatgca aggcatgatg aaacaagtac aaaaaatgca aaaagaaatg gcagaagcac
    21961 aagagctgct aaataaaaca gagtttacag gtaaagccac caatgaattg gtcaaagcgg
    22021 tttttacagg cgatcgtcgc atgaaagata tccaaatcaa tgaagaagtc gttgatcctg
    22081 atgatatcga gatgcttcag gatcttgtcg taatggcagt caatgatgcg atcgcacaaa
    22141 ttgatgtgga aactgagaaa aaattaggga aatacgcaaa aggtttgcca ggattttagt
    22201 tttaaactga aggagtaatg aatatgcaat atccagaacc aattgcgaaa ctgatcgaca
    22261 gctacatgaa acttccagga atcggtcaga aaacagcgac aagactcgcc ttttatacga
    22321 tcgatatgaa agatgaagta gtaaatgaat ttgctaaatc tttgttaagt gtaaaacgcg
    22381 atttgcattt ttgtagtatc tgcggaaaca tcacagaaga agatccctgc gaaatatgta
    22441 gaaatccttc acgtgatcga agcgttatcc tagtggtcga agagccgaaa gatgtaatgg
    22501 caatggaaaa aatgcgagaa tatcacggac tttatcatgt tctccatggt gtgttatcac
    22561 cgatggaagg gacaggtcca gaagacatca atatttcttc tctgttgcag cggctgcatg
    22621 atgcagatgt aaaagaagtg attatcgcaa caaatgcaac aacagaggga gaagcaacag
    22681 ctatgtatct ctcacgtctg atcaaacccg caggtatcaa agtgactcgt ctagcacatg
    22741 gtctgtctgt aggctctgat atcgagtatg cagatgaagt tactttattg aaagctgtcg
    22801 aaggaagacg tgagttataa atttctaatt aagtgttttt tcagatacaa tataaaagca
    22861 gcacaagaga tgggggcaac aatgtgaacg gactatttat tacaatcgag gggcctgatg
    22921 gtgcaggaaa aaccagcatc ttgaatgaac tttaccctct tttgaaagaa gtagcaaaaa
    22981 cagaaatcat tcaaacaaga gaaccaggag gcattccgat tgctgaagag atccgagcag
    23041 tgatccttga tccaaaaaat gatcggatgg atgaacgtac ggaagcactg ctttatgcag
    23101 cagcaagaag acaacatcta gtcgaaaagg ttctgcctgc tctggcaaaa gggaaaatcg
    23161 ttttatgcga tagatttgtc gacagttctc ttgcttatca aggtgcaggc cgacgcatcg
    23221 gcgttaccga gattgctcgg ttgaatgagt ttgctacaga aggtactacg ccggatttta
    23281 ccctttatct tgacgtagat tcagacaccg gattaagaag aatcaagaaa aacagacaaa
    23341 atcaaatcga ccgtttagat tcggaaggtt tggaattcca ccagcgtgta cgtcatgctt
    23401 acttaaagct ggcagaagag aatccggaaa gaatccacaa agttgatgca agaaaaagct
    23461 ttgaagaagt gctgcaaaca agttatcata caattattga acagtatccg caattttttg
    23521 aaaactagga aggtgaacgt ttatgaaaat tattttagcg attatccaag acaaagacag
    23581 caaccgttta tcaaatgaat tgattgatgc aaatatccgt gccacaaaac tatcatcgac
    23641 aggcggcttc cttaaggctg gaaacagtac gtttatcgtg ggtattgaag atgaccgtgt
    23701 agaagaagca ttggaaatca tcaaaaaaac gtgtgaatca agaaagcaat ttgtatctac
    23761 acctgttaca ctggatattt cgatggatgg gggcgtccct tatccagtag aagtagaagt
    23821 aggcggtgcc acagtcttcg tcttacctgt tgaaggattc catcaatttt aggaggagct
    23881 gagctatgaa tcaagaaata aacttggctc aaatgcaacc gatcgtgtat cagcagcttc
    23941 aacggagttt tgagcatggg cgtatcgctc atgcttatct ttttgaaggg gagaaaggaa
    24001 caggaaaaca tgaagtaggt atatggcttg cacagcacct gttctgtacc caaatgaaag
    24061 acaatctgcc ttgtggaaaa tgcaacaatt gtcagcggat cgaaaagaaa gaacatccag
    24121 atgttttggt tattgaaccg gaaggacaaa ccatcaaagt cgatcagatc agacgtttgc
    24181 agacggaatt cagtcgcagc ggttacgaat caagaaaaaa agtttttttg atcaaagaag
    24241 ctgaaaaaat gaatagcagt gcggcaaaca gtttgttgaa attcttggaa gagccccctg
    24301 gagatttttt agcggtgtta gaaacagatg cagtaggaag aattctccct actattcaat
    24361 ctcgttgtca agttctgcat tttcaagaac tttcgaaaga agcactgatc catcgattgc
    24421 aagaagagca gattccattg gaaaaagcaa aacttttggc tttcttaaca aacagtttag
    24481 gaaaagcagt tgaaatctct caagatgaat ggtttaatga tgctaaggac ttgatttatc
    24541 aatggttcgt ttacttgcaa aagcaagata ctcaagcctt tatctatgtc caaaaaaaat
    24601 tggtaaaatc atttaaagaa aaaacacaac agtttacagg attatctgtc ttgatgtttt
    24661 attatcagga agcacagcgt caagcagtag caaacggcga atatggcaaa gtgaaacaga
    24721 taaaccagat aattgaacgg atattgatcg cagagcagaa actacgcagc aatgtcagtt
    24781 ttcaggctgt tgcggaacag ttcgttttgc atacgatttt ttagcttgcc gcaaaggatg
    24841 atgatcaagg aggatggaaa tggtagaagt agtaggtgtc cgatatagag atgcaggtca
    24901 tatctattat tttgcaccag gagactctga atatgtttat agtgacaagg tattagtcga
    24961 atctcagcaa tccttacata ttgctactgt tgtccttcct aaaaaagaga ttgatcccaa
    25021 tgaccttcct gaagctatcc gtccgatctt gcataaagcg tcacaagctg aattagaaaa
    25081 agacaaaaaa aacaaaaaag atgcttatga tgcattgaaa atcgcaaaag aaaaagtgca
    25141 gttgcgtgag ttggaaatga aaatggtcaa agccgaatac acatttgatc gaaataaatt
    25201 gattttttct ttcacagcgg acgggcgaat tgactttaga gaattggtaa aagatctggc
    25261 cgctgtattt cgtactcgta ttgagcttcg acaaattggt gtccgcgatg aagcaaagat
    25321 aataggcgga attggtccat gtgggcgtcc attatgctgt tcttcttttt taggcgattt
    25381 cgtgcctgta tcaatcaaaa tggcaaaaga tcaaagttta tctttgaatc caacaaaaat
    25441 ttccggttta tgcggacgct tgatgtgctg tctgcaatat gagagcgaag aatacgagac
    25501 agcgaaaaaa gaactccctg actttggaaa agtaattgct acacctgatg gtaaagggaa
    25561 agtcgtaggg ttgaatctgc ttagccgtat cgttagtatt cggctgactg gacgggaaaa
    25621 tcctgtcgaa tatgaatggg atgaattgaa agaatttatc caagtaggtg agatgaatgg
    25681 ataagagatc attatacgac ggattgaatc aattagaatc agagttgaga aatacattaa
    25741 cagaacttgt tgaaatgaag catgctttgc atgagatcgt agaaaaaaat acaactttgg
    25801 agatggaaaa tcaacgtctg cgggaacacc tgcgggaatt gaaccaagtt gcacaagctc
    25861 cagctgaaaa tgtgaagcag gaactttcta agtctcgtat gaatcttgaa aaaatttatg
    25921 aagaaggatt ccatgtctgc tacgatttat atggatcaag aagggacaat gacgagcctt
    25981 gtgcgttttg tctagaagtg atctatcgcg aaagcggtcg atgaccaata acaagaggag
    26041 actggagcgg agaaatactg ctccagtctt tttattaagg agattgtgac agatgtataa
    26101 tcaaaaaagt ttcaagggaa atccggtata tggggattta tatcttgtac cgacgccaat
    26161 tggaaattta gaagatatga gctacagaag tgttcgtatg ctgcaagaat ccgatcttat
    26221 cgcaagtgag gatacgcgta atacacaaaa gttattgaat cattttgaaa tccatacacc
    26281 ccaaaaaagt ttacatgaac ataattataa agaacgtgtc cctcaattga ttgctttgtt
    26341 aaaagaagga aaaacgattg ctcaagtcag tgacgcagga atgccttcca tcagcgatcc
    26401 tggacatgag ctagttttag cctgcataaa agaagggatt tctgtgatct ctatcccagg
    26461 acctacagct gggatgacgg ctttgattgc ttcagggcta gtgcctcaac catttttatt
    26521 ttatggcttc cttccacgta agaaaaaaga acagaaagaa acattgaaaa agctaaaaga
    26581 acagacagtt acgctgattt tttatgagtc accttatcgg atcactgcta cattgacgaa
    26641 tttcttagaa atttttggaa atcgccaagt cgttttgtgt cgggaattga caaaggtcca
    26701 tgaagaatac cttagaggaa gtacggaaga gctactcgct tatatcgaag aacatccagt
    26761 aaagggtgaa tgttgtctgc ttgtagaagg gaatttgttc gaaatgccag tgacggaagt
    26821 tgatgaagag ttgggaacgt taaaggaaca agtagaagca aagatcgctt caggcgaaaa
    26881 accaaatgct gctatcaaag cagtagcgct gaaaaatggg ctgaaaaaac aagaagtcta
    26941 ccgtcagtac catgaattag actgagatag tataagttgt ctataatgaa agactaatga
    27001 ggaaatcagc tactaacttt tcaaatctct tgaacgcgaa cgaatgttct tgtataatga
    27061 ataagaggtg aggatgatgg agctgcaatt tcctttacat aatgatacat caagaaaaat
    27121 cattcatatt gatatggatg cttttttcgc ttcggttgaa gaacgagatc atcctgagtt
    27181 tcgcggacat ccattagtaa ttgccagaca ccccagtgac actggtgggc gtggagtagt
    27241 gactacagca aattatgaag caagaaaatt cggaattcat tcagcaatga gtgcacaaaa
    27301 agcgtatgag ctttgtccgc aagccatctt taagcctggc gaccatcata aatacagcga
    27361 tatttccaaa caagtacggg aagtattcta tcgttataca gatattgtgg aaccagttag
    27421 tatcgatgaa gcgtatcttg atgtgaccga aaataaaatc aattgcaagt cagccatcaa
    27481 aatcgcccga atgatccaag cagatatttg ggagagtgtc cagctaacat gttctgcggg
    27541 agtaagttac aataagtttt tggccaaatt agcatcagac tatcaaaaac ctagaggaat
    27601 cacggtagtt gcccctcaag acgctgtgcc atttttaaaa gctttgccaa ttgaaaaatt
    27661 tcatggggta gggaagaaaa ctgtacctag aatgcatgag ctagggattt atacaggaaa
    27721 agacctatat gaatgcacag aaatgatgtt gattcgaaat tttggaaaaa tgggctattc
    27781 tctttatcgt aaagtcagag gaatccatga ttctccagta aacgttacaa gagagcgaaa
    27841 atctgtggga aaagagcata cttatgggca gccgttgcaa acggaagaag cagtgctgac
    27901 tcagttgcgc caactagctg aaaaagtaga agaagcactg agaagagtcc aaaaacatgg
    27961 gaaaacagta gttttgaagg tcaggtacac ggactattct actgtgacca aaagagtgac
    28021 attgccagaa tatatctata aaaaagaagc actcttttat caagcaagcc ttatatggga
    28081 ggaaatcttg ggagtagaaa aaggaatccg attgttaggg attactttga cgaatctgga
    28141 cccgatgact tatgaaaaca tcgtccttcc attatgggaa aatcaagaaa tataaaaaca
    28201 gctttgagag ttgaaaccgg aaatccgttt tctctcccaa agctgttttt atatcaagtc
    28261 ttgctttttg atttgttcat aggtttctgt tgcatccgta catttttccc aaatcacaac
    28321 ttgattggat tctaaagatt tttggacgtc aatgatccgc tggttgctac ttccgcgaaa
    28381 ttgtagtttt aggtcacgtt tgcttaattc aaagcgtccg tcaacaagga tatcgatttg
    28441 cgaaaggagt tctaatttat cttctgaatc ttgtagcaat tcatcaaagg tatagcctgt
    28501 ccatgaccag atatcttttg tcgtaccgaa ctcttttcgg atacgtcgta cgacttgcag
    28561 acaaacatct gtattcaaaa aaggttcgcc accaagtaaa gttaatcctt gcacgtaatc
    28621 gttgcgcaag tcagcgatga tttgttcttc taattcaggt gtgaatggtt tgccatatcg
    28681 aaaattttgt gcagctttgt tgaagcagcc ttcgcaggca aataagcagc cactaacgta
    28741 aagactgttg cggacacctt caccatcgac aaaattaaac ggtttgtagt cagcaatata
    28801 gttttggctg tagtcttttg ctaaccattc ttttggtttt ggatttctca taaaaaaact
    28861 tctttcattt aaggttcaaa gatatagcta ttgaagataa aatctactcg accacataaa
    28921 taagaacaaa ctgacctaaa tccattgttc aagaaagctt gttcttattt tgatagaggg
    28981 cgttcaaaga tacaaagcca tcgaaagagg gtggtgaaaa aagacttgtc atagcctctt
    29041 attctgaata aacggtgttc aaaagcttgc atctgcagta ataagcccaa ctaaacgaaa
    29101 aatatgagga actatttttg tttagctatt cttattactt gatgcaggac agctttttca
    29161 caacctcatt tcatgtgttt cacacgtgag gaaatttctt tatggcggcc atgtaccatc
    29221 ggacgagctt gcggatttcc taaatagcca caagtacgtt tgactacatc acatgttttt
    29281 ggatcatgat ttccacattc tggacattcg aaccctcgtt ctgtcggttt gaaatcacct
    29341 tcgaaaccac attgatagca atgatcgatt ggcgtattcg taccaagata cccgattttg
    29401 tcatatgcaa aatcccatac agcttctaaa gctttcggat tttgttggag catagggtat
    29461 tcacagtagt ggatgaatcc acctgaacaa tattttggat aatccttctc aaattccaat
    29521 ttttcaaatg gcgttgggtt tttacgcaca tcatagtgga aactgtttgt ataatattct
    29581 ttatctgtga tattttcgat gatgccgaat ttttctgtat ccaaacggca gaaacggtca
    29641 gttagacttt cacttggagt tgaatagaca ctgaaatgat aaccatattc atccccccat
    29701 gcatctgcat tagcttttaa ttctttgacg atatcaagtg tgaaagcttt tgcttctggg
    29761 tttgtttccc attcgccacc gaagaaagct gaagcaactt catacaatcc aatgtagcct
    29821 aacgatacag tagctcgacg gtttttgaat aattcattga cagaatcggt acgtgaaaga
    29881 cgtttcccga aagcaccata catatataaa attggcgcat ttgctggaat ggcttcttta
    29941 caacgttcta cacgatagag aagcgcatct ttcatgattt ctaagcggtc agacagtaac
    30001 tgccagaatt tattctgatc tccttttgat tcgatcgcga ttcttggcaa attcaatgta
    30061 acaacaccta aattcattcg tccgacattt acttcttgac cattttcgtc tttccaccct
    30121 tgcaagaatg aacgacagcc cataggtact ttgaaacttc cagttaattc aacgatttta
    30181 tcgtaattca gaatatcagg atacattctt ttcgttgcac attccaatgc tagttgtttg
    30241 atgtcgtaat taggatcttc agggtttaag ttcacacctt ttttcaaagt gaagatcagt
    30301 ttagggaaga tagctgtccg cttttcactc cctaatccat tgattcgaat ctgtaaaatt
    30361 gctttttgaa tttctctttc aaaccagttt tctcctagtc caaatcctaa tgaagtgaaa
    30421 ggagtttgtc catttgaagt aaataaggta ttgatttcat attccaaact ttgcatagca
    30481 tcgaagatat cttttttggt ttttgctttt gcatattctt tttggcgttc tggcgaatcg
    30541 atccattctt ccgcgtcttt tagatgtttt ttatagttca attctgcaaa aggagcaagc
    30601 aattcatctg ttcggtcggc agaacagcca ccatattgac ttgacgctac attggcaata
    30661 atctgtgaga tttgtgctgt ggcggtctgg atagattttg gcgattcgac ttcggcattg
    30721 ccgattttga acccattgtt cagcatgcct ttgaaatcaa tcaagcagca attcgtcatc
    30781 ggtgtataag gatgataatc caaatcatgg taatggatat cgcctttttg atgcgcattt
    30841 gctatatgtg gtggcaacat tttcagtccg attgattttc caacgatccc ggctgtcaga
    30901 tctcgctgtg tattgaaaac attactgtct ttatttgcgt tttcgttcac gacagtttgg
    30961 tccttgttga ttagcttttc aatcgaaaag ttgatatctg ttgctttact gcgagcaaaa
    31021 tctcgtttgg tacgataatt gatgtactct tctgctaatt catattcgtt tttttctagt
    31081 aatatatgtt ccacgatgtt ttgaatctca tagattttga cattttcagc aaaccggctt
    31141 ttgatttctt ggacaaccaa gtctgcaatc atttgaattt tttcataagt aagtggtgat
    31201 aatgagccgt gaattttctt ttctgctttg atcagtgctt cattgatctt ttgttcatcg
    31261 aaatcaacat ggcggccatc gcgtttgatg actttcatct gggatgtctc tggctgctga
    31321 gtaacgacag ttttttcttc ataatttact ttcattatct tcaactcctg ctaaattgtt
    31381 cttgtctatc ataaaacaaa atcggggaag gagcaactaa atatcggcta tatatagtat
    31441 tcaatgtgct gtataattgg atttctatac tatatgtagt gtatatttta ttgtgaaaaa
    31501 aggtgaaaac ttgtagctgg tggatttgtt ttttgcatat ttaattttgc tgaataaaat
    31561 aattgaataa aattttttat tcagtccaag taaaagtgac aaaaacataa gagcaaatta
    31621 ttcgtagata tgctactatg caaataaata gaaatgaaaa atgaaagagg aggagaagaa
    31681 ttggaaaaga tgatcgaagt gaaacatcta agtaaaagct acggcagcaa actttctccc
    31741 gtcaaagcct tagataatat ctcattcaca gtagaagaag gcgagtttgt tgggattatg
    31801 ggcccaagtg gtgcaggaaa gacaacgctg atgaatatct tgtccacgat caatttgcct
    31861 acgatgggca gtgtctcgat ccaaggaaaa gaaatcacaa aaatgaaaaa cagtgagctc
    31921 agtgactttc gtcgaaaaaa actaggattc atttttcaag agttcaattt gatcgatacc
    31981 ctaaatgcaa aagacaatat cctattgccc ctagcagttg aacggatgac taaagaggaa
    32041 atggaaaaga gagtaagaca tgtagctcaa ttgttgaata tagaagaact gctcaaacgg
    32101 tatcccgatg aactttctgt cggacagaga caacgaattg cagctgctag agcacttgtt
    32161 gttcagccac aagtgatttt tgcagatgaa ccaactggct ctttggactc gaagtcggct
    32221 acagaattgt tgaactactt gaagaccatg aatcaaaaag aaaaagcaac gatactgtta
    32281 gtcacccatg atccatatac agcaagctac tgcgatagaa ttttatttat taaagatggt
    32341 gtgatttttt ctgaagttgt cagaagaggg acaagaagag agttctttga gaaagtaata
    32401 gacatgcagg cgacgattgg cggaggagga aaaatgaatg ctgtataaac tagccgttaa
    32461 aagttttttt cggcagtcac gaggctatct tgtttatttt ttcagtttga ctttatccgt
    32521 aatgatctat tattccttca gcgcaatgac ctacgatcaa tctttagtca gaagagcaag
    32581 tcaagatact tcgatagatg gtggattgag ttttggtgga tttatcatta ctgttgtttt
    32641 gctttttttc gtattcagtg ccaaccgatt tttcttgaat agaaggcaaa aggaaatagg
    32701 catctatcag ctttttggta tgaataaact gcagatatca ggtatatatg tgttagaaat
    32761 catgataatt ggattgttcg cctgtatttc tgggatatta ctcggtatta ttttttccaa
    32821 actcttttca atgattttgg ttcgaatgat ggatatggat cttactagtc cattttttat
    32881 ctccattcca tcagtagtag atactttatt tgcttttttc atcatccttt tagcagtatc
    32941 tgtatattca atatggaaaa tatggcgcta tccaatgatt cgaagttttg gagaaaaaga
    33001 acaagcagat agttcaacaa tgcgcttcag gacaaggcac cgattactcg cagtcatcgg
    33061 gttactactt ttaacgagcg gatattttgg agctagtcat tttagggaaa tcacttcatg
    33121 gatgatcagt tatcattatc ttgatacatt agttttcttt tttccttttt taattttttt
    33181 cgtttgtgtg gtcggtactt atttgttttt ctgttataca tttcggttat ttttgatcat
    33241 attgagtaaa tggaagattt attactatgg tatcaatttt ttgataatgg gtaacacgca
    33301 agttcatttg ctcaaaagtt ggcgtacaaa ttctctgatt acagtcattc tcggaatttc
    33361 tttaatgaca attggcggca cggctagtat ctctactatt ttatcctacc aggagaaagt
    33421 ttcctccccg atggactatc agtttgatgt tgagacagag aaaaaaatca agccgattct
    33481 agcagaggaa gggcagaaga taactaaaga aatcaccttg cattataaag tcattgggag
    33541 tatctatagt caaaatctcg aagaacctat gtttcaaact gctaatctga tcacggaaac
    33601 acaatatcaa gccttcagaa aagttaatcc agatttgccg aacatttctt tgaaatcacc
    33661 tggtcatact gttttgcttg atcaaatgca aactcttttt cggaatattt cattatatgg
    33721 atctagtatt ggactgccag ataatcaaga attgaagatt caacaaatgc ttccaagtgt
    33781 attaggtgac gaaacaatgg tatatctaag tcctgtactg attgtagatg ataccgtttt
    33841 tgaaaaagca aaaggtgtgg attatcaagt agtcaatgta gatgtatcag gtggaaataa
    33901 tgaaaaaata gatgaacgac tgagcaatga cctgtcgata aagtggctca atgctgttta
    33961 ttattcttat gatatcgtaa atggaagtct acaaggagaa attagcacaa agaaacttcc
    34021 ggatgaccaa atgaaggaag cacaatttag ccaagaaagc tctcgtttga atttctcttc
    34081 cagctattcg aaattgcgtg cagttaatcg gcgaatcggt atcaatattt ttgtcacact
    34141 atttgtcggg atgatcgtga ttattgctac tggtagtatt ttgacagtcc ggcaattagc
    34201 agaagcagag gaagaaaaag aaaattatca cctattgact aagctgggga ttccacaaag
    34261 aaggatcaga cggatgatct tcgagcaaaa cgcacttacc ttctttccac ccatgatatt
    34321 agggatcact cacgcagtat ttgcaatcaa tgtcttcact cagtatataa aaactgccga
    34381 ttactggttg gcttatcttg tgagtggctt attgatcctt atctatcttt tgctttatgt
    34441 cattacgagt cagttatatt gtcggattat cgaggaatga tatacggaat aagacaagat
    34501 aataaataga actaaatttt gaataccatt tatttggaat aagaggttgt gacacgattt
    34561 ttgtcacaac ctctttattt tttatatttt ttaaaagtac gagaaattat aaagaaaaaa
    34621 gcagtgattt gatgtgctat ttttataaaa gctatgtgac aaaactgtta gtaaaaaaac
    34681 ggtttctgta agctagacac atgtttcttt ttgtcaaaat tagttacact ttgt
//
LOCUS       DAZKMK010000018        34704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000018 DAZKMK010000000
VERSION     DAZKMK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.018"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            608..1297
                     /locus_tag="ACG8V1_000928"
     CDS             608..1297
                     /locus_tag="ACG8V1_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0329042.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1284..2492
                     /locus_tag="ACG8V1_000929"
     CDS             1284..2492
                     /locus_tag="ACG8V1_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0329043.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2786..4057
                     /gene="serS"
                     /locus_tag="ACG8V1_000930"
     CDS             2786..4057
                     /gene="serS"
                     /locus_tag="ACG8V1_000930"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0329044.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4606..6090)
                     /gene="guaB"
                     /locus_tag="ACG8V1_000931"
     CDS             complement(4606..6090)
                     /gene="guaB"
                     /locus_tag="ACG8V1_000931"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0329045.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6595..7290)
                     /locus_tag="ACG8V1_000932"
     CDS             complement(6595..7290)
                     /locus_tag="ACG8V1_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0329046.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7380..8480)
                     /gene="ychF"
                     /locus_tag="ACG8V1_000933"
     CDS             complement(7380..8480)
                     /gene="ychF"
                     /locus_tag="ACG8V1_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0329047.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8489..8680)
                     /locus_tag="ACG8V1_000934"
     CDS             complement(8489..8680)
                     /locus_tag="ACG8V1_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0329048.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8701..9591)
                     /locus_tag="ACG8V1_000935"
     CDS             complement(8701..9591)
                     /locus_tag="ACG8V1_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0329049.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9578..10345)
                     /locus_tag="ACG8V1_000936"
     CDS             complement(9578..10345)
                     /locus_tag="ACG8V1_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0329050.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10357..11070)
                     /gene="rsmG"
                     /locus_tag="ACG8V1_000937"
     CDS             complement(10357..11070)
                     /gene="rsmG"
                     /locus_tag="ACG8V1_000937"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0329051.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11302..13203)
                     /gene="mnmG"
                     /locus_tag="ACG8V1_000938"
     CDS             complement(11302..13203)
                     /gene="mnmG"
                     /locus_tag="ACG8V1_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0329052.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13220..14617)
                     /gene="mnmE"
                     /locus_tag="ACG8V1_000939"
     CDS             complement(13220..14617)
                     /gene="mnmE"
                     /locus_tag="ACG8V1_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0329053.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14916..15299
                     /locus_tag="ACG8V1_000940"
     CDS             14916..15299
                     /locus_tag="ACG8V1_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329054.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15467..16225)
                     /gene="jag"
                     /locus_tag="ACG8V1_000941"
     CDS             complement(15467..16225)
                     /gene="jag"
                     /locus_tag="ACG8V1_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0329055.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16243..17061)
                     /locus_tag="ACG8V1_000942"
     CDS             complement(16243..17061)
                     /locus_tag="ACG8V1_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0329056.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17058..17414)
                     /gene="rnpA"
                     /locus_tag="ACG8V1_000943"
     CDS             complement(17058..17414)
                     /gene="rnpA"
                     /locus_tag="ACG8V1_000943"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0329057.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17532..17666)
                     /gene="rpmH"
                     /locus_tag="ACG8V1_000944"
     CDS             complement(17532..17666)
                     /gene="rpmH"
                     /locus_tag="ACG8V1_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0329058.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18189..19523
                     /gene="dnaA"
                     /locus_tag="ACG8V1_000945"
     CDS             18189..19523
                     /gene="dnaA"
                     /locus_tag="ACG8V1_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0329059.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19721..20851
                     /gene="dnaN"
                     /locus_tag="ACG8V1_000946"
     CDS             19721..20851
                     /gene="dnaN"
                     /locus_tag="ACG8V1_000946"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0329060.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21080..21322
                     /gene="yaaA"
                     /locus_tag="ACG8V1_000947"
     CDS             21080..21322
                     /gene="yaaA"
                     /locus_tag="ACG8V1_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0329061.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21309..22433
                     /gene="recF"
                     /locus_tag="ACG8V1_000948"
     CDS             21309..22433
                     /gene="recF"
                     /locus_tag="ACG8V1_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0329062.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22430..24376
                     /gene="gyrB"
                     /locus_tag="ACG8V1_000949"
     CDS             22430..24376
                     /gene="gyrB"
                     /locus_tag="ACG8V1_000949"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0329063.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24396..26867
                     /gene="gyrA"
                     /locus_tag="ACG8V1_000950"
     CDS             24396..26867
                     /gene="gyrA"
                     /locus_tag="ACG8V1_000950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0329064.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27103..27402
                     /gene="rpsF"
                     /locus_tag="ACG8V1_000951"
     CDS             27103..27402
                     /gene="rpsF"
                     /locus_tag="ACG8V1_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0329065.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27452..27964
                     /gene="ssb"
                     /locus_tag="ACG8V1_000952"
     CDS             27452..27964
                     /gene="ssb"
                     /locus_tag="ACG8V1_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0329066.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27990..28226
                     /gene="rpsR"
                     /locus_tag="ACG8V1_000953"
     CDS             27990..28226
                     /gene="rpsR"
                     /locus_tag="ACG8V1_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0329067.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28369..30342
                     /locus_tag="ACG8V1_000954"
     CDS             28369..30342
                     /locus_tag="ACG8V1_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0329068.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30348..30800
                     /gene="rplI"
                     /locus_tag="ACG8V1_000955"
     CDS             30348..30800
                     /gene="rplI"
                     /locus_tag="ACG8V1_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0329069.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31074..32441
                     /gene="dnaB"
                     /locus_tag="ACG8V1_000956"
     CDS             31074..32441
                     /gene="dnaB"
                     /locus_tag="ACG8V1_000956"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0329070.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33221..34513
                     /locus_tag="ACG8V1_000957"
     CDS             33221..34513
                     /locus_tag="ACG8V1_000957"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0329071.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10575 a         6747 c         6860 g        10522 t
ORIGIN      
        1 aaaggtctac ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta
       61 tatcatatca gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt
      121 tttcgaaatc ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact
      181 tttttgaagt ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag
      241 ttatcttatc atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta
      301 ttggtgttct tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa
      361 atgcttattt atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg
      421 ttcgtcttta taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt
      481 cttgtcttat tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct
      541 gcttttatct atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga
      601 tttatttatg aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat
      661 ctatgttcac aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc
      721 taaaatcaga acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat
      781 ggatgggatg caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct
      841 gacagcaaaa actactgata tggataagat caaaggactg gtcgctggtg cagatgatta
      901 tgtgaccaag ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg
      961 tacaaaaatg cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat
     1021 caataaagac tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt
     1081 ggaattcggt atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga
     1141 aatttttgag cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca
     1201 cgttagtcat ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac
     1261 cgtttgggga gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc
     1321 gtcggatcaa tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga
     1381 ttatcctttt gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca
     1441 gttcaccttc tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga
     1501 acactgattt gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata
     1561 ttggtgtttt gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca
     1621 tcagcgaact tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga
     1681 gtggtgactt aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag
     1741 cgattgaaga cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc
     1801 atgatatccg taccccactc acgtccatca ttggatacct aggactgata gaagatggcc
     1861 agtatcactc agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc
     1921 agatgaaatc tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc
     1981 cggtgaattt ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg
     2041 agttggaagc ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta
     2101 tcatggatgg cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt
     2161 tgaaatacgg aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag
     2221 ttgttgcaac agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat
     2281 tcgaccgttt ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg
     2341 gtttagcaat cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag
     2401 ataagcaatg gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta
     2461 ttgatacaca ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt
     2521 agaataaatg aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca
     2581 ggtgtaaatc agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat
     2641 aagaaaggtc ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg
     2701 gtggtaccgc gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt
     2761 ttattttcaa aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga
     2821 agaagtacaa gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat
     2881 ggaactagat gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg
     2941 aaatgatgtt tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa
     3001 aatcgctgaa atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga
     3061 agtggatgaa aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt
     3121 gccagttgga acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac
     3181 atttgctttt gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt
     3241 tgaacgtggc gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg
     3301 attagaacgt gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac
     3361 tgaaatgatc actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc
     3421 aaaattcaaa gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc
     3481 tgaagtacca ttaactaact attataatga tgaaatttta gatggtgcag atttacctat
     3541 ttatttcact gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac
     3601 acgaggactg attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt
     3661 agagcattct tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa
     3721 attgcatttg ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc
     3781 taaaacttat gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc
     3841 ttgttctaac tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga
     3901 tggaaaaatc cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt
     3961 agctgccatt ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct
     4021 cgttccttat atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta
     4081 gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga
     4141 cttaataccg agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa
     4201 ttaacctgag tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg
     4261 aactaatacc gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc
     4321 cccagctcct agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg
     4381 tacttgtgat tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa
     4441 gcgtttaact ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa
     4501 tttcttctta tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt
     4561 gagacaaaag tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga
     4621 atagtttggt gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact
     4681 gccgctcatt tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac
     4741 ataacccatt cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc
     4801 tttataagca acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact
     4861 accttggaag taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg
     4921 gtatgttttg aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc
     4981 tgccaacata cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc
     5041 accagaatat ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga
     5101 tgctgcatca taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt
     5161 acaaatcgat cctggtccga taccaacttt tacgacatct acacctgcat catataaagc
     5221 ttttgtcgct tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga
     5281 acggatttct tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat
     5341 gattgcatct gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac
     5401 accaacggct gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc
     5461 gatcactttc tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac
     5521 gattggtaat ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt
     5581 acctactggg gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata
     5641 atctgttaca aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag
     5701 tgtttcaacg attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc
     5761 tacaagatgc tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt
     5821 gacttttctt acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag
     5881 tccgccttgt cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc
     5941 actgatgatt ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc
     6001 gtttggtaac acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt
     6061 tttcgtgaac tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat
     6121 ctattaaaat aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt
     6181 tctcatagaa taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa
     6241 agctgtcctg catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa
     6301 ttggttgggt ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg
     6361 aaaaagctgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt
     6421 tgcataaatt ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg
     6481 gactgtgaca agacttttgt cacagtccct tactattatt taactatgga taaacaaaat
     6541 gaggttcttt actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct
     6601 tcttgggacg gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc
     6661 taaagcagtt gccccgataa tgataactac tactggatct aatactggat tgataactgg
     6721 tggcaatagt gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat
     6781 aagcaatgtc ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg
     6841 atagatgtat ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag
     6901 tgtagaaaag ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat
     6961 gatacttaat agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga
     7021 tgaatttttt acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc
     7081 aaacaattga cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc
     7141 atgaagggct tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa
     7201 atcaaaaatg tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt
     7261 tacgattgct cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt
     7321 tctcaacggt cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt
     7381 tacacattaa agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct
     7441 aatcgaacac gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca
     7501 aaagagacag tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct
     7561 gcttgtggtg ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca
     7621 gtaaagtaag tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct
     7681 gattcttcga ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg
     7741 atttcttctt ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag
     7801 ttgcgcactt cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca
     7861 ttggctacat aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt
     7921 tcttcttcag taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt
     7981 ttatccagta cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct
     8041 acacgagtat agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc
     8101 gtatcaatat cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca
     8161 aaacagcgaa caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt
     8221 cccaaacctt ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa
     8281 gtcgtcggaa cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca
     8341 ggtacttcta ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct
     8401 gctcctgctt ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca
     8461 attccagcag tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt
     8521 tttttaattt cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac
     8581 atctgatttt gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt
     8641 gaggtttttt catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt
     8701 ttattcttca tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga
     8761 aagatattca atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc
     8821 aaatttatcc atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag
     8881 ccttgggatt tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc
     8941 cagttggcgg acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt
     9001 tttatttttt aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac
     9061 catttccttg acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct
     9121 gctctttcct aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat
     9181 ctcatatgct tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac
     9241 tgctacttgc atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt
     9301 ttcagctagt ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac
     9361 tgcggaacgg cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag
     9421 ttcctgcaaa gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc
     9481 attcaatggt agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa
     9541 atcttgaaac agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca
     9601 cttcctttgc caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga
     9661 tcggttttcc atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt
     9721 aaactttttc gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc
     9781 gtgcatcata cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt
     9841 ggactaaacg aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact
     9901 gaacaggtat caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag
     9961 aaggcgggca atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt
    10021 ttaatcttga ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga
    10081 gagtcgcagg cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg
    10141 gcaattcatt gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga
    10201 caccactggt agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc
    10261 tagcaagaca agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt
    10321 tcgcaacaga tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg
    10381 cagtccgggc tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat
    10441 atgccgttga tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc
    10501 taataaagca atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa
    10561 tgcataaaaa tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa
    10621 acgagcaacc gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc
    10681 aaatgtttct gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc
    10741 agataagaag gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa
    10801 ggcaattttt aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa
    10861 aggttgcgta aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata
    10921 tacgtctttt tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt
    10981 ccgataataa atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc
    11041 caaggattgg cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt
    11101 gtttcacaga ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat
    11161 ggattcttaa aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact
    11221 cataaaatat acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc
    11281 tcttctattt attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc
    11341 gatatagacc attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg
    11401 ggcaattgtt tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt
    11461 gattgctgcg tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc
    11521 tactttttcc atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc
    11581 gatttctttt cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt
    11641 aatctcagga cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt
    11701 aatcgttgct aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg
    11761 tttgatttct tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt
    11821 tactaagcct acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa
    11881 aagcagacgg tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt
    11941 taccaaatca tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc
    12001 ttttccctga actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc
    12061 ttcataaccg cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt
    12121 ttctaatgtc ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt
    12181 acgcatcatt tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc
    12241 ctctggtaaa gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg
    12301 ttccaaaaat aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga
    12361 tggacaatag cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa
    12421 gttgtctcga ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg
    12481 atctagttta taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc
    12541 aggctgctct tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc
    12601 tgttttgaac cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga
    12661 aggttgagag ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg
    12721 aagtgctgtt ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt
    12781 cactactcca tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag
    12841 cgtcaaattt ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc
    12901 tgcttgtgct cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg
    12961 gatataggtt ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac
    13021 gacaacgcct ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc
    13081 caaatttatt gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc
    13141 tgaaccggcg tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc
    13201 caatcgtttg ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta
    13261 gctcatcttg tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat
    13321 ctatttgaac gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg
    13381 agagggaagc tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc
    13441 tttctcctgt ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc
    13501 cgtcgttttt agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt
    13561 tttcttgttc cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc
    13621 cacttgtcgc ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa
    13681 caagtaagat caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt
    13741 ccaccacatc ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc
    13801 gaacattgac atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg
    13861 ctttttcttc tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga
    13921 tgattgccgt acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg
    13981 cttggattct ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt
    14041 cgacatcatc atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa
    14101 tctcttgtct caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta
    14161 gtcccattgc tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa
    14221 ggtcaacacg accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg
    14281 caccttctct taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat
    14341 tgatttctac tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga
    14401 cttcatcgac aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac
    14461 ttggtacttc tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag
    14521 cttgatcgcc acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg
    14581 ccgcaatcgt atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt
    14641 gcgcacaaaa aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt
    14701 tgactgcttg ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca
    14761 agtgtttaaa acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat
    14821 tgtccttgga tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca
    14881 aggctttatc tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca
    14941 cttttgaaag gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg
    15001 accctcggat caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc
    15061 gcgatatcgt ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca
    15121 aagatgacct tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg
    15181 cgaatgcagt aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt
    15241 ttctggaact ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa
    15301 tgaaacagac aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc
    15361 aaagttagtg actacttcat cgtatactct gataagaatg atttctagta aaatatactt
    15421 ggtttgaaaa actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt
    15481 tagcaggttc tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat
    15541 gttcatcttt gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta
    15601 agaaaacagg ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa
    15661 tgatctcttg gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc
    15721 ttgctaaacg atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat
    15781 gtttcccaat cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg
    15841 attctctttc cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat
    15901 aaactgccaa ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt
    15961 cttcaagaat cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt
    16021 cttctacagt ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa
    16081 gccgtgcatt ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga
    16141 tttcgacttg gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta
    16201 ttgttgcagc ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt
    16261 aggattcatt gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc
    16321 acgctctttt cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc
    16381 attggaaact acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat
    16441 cattacaggc atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat
    16501 gctagataaa tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt
    16561 tggatccgcg ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg
    16621 tgaaatagat tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc
    16681 agcatatgga ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact
    16741 ttgtgtttcc ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa
    16801 ttcttgcgtt ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg
    16861 gatgatcagt gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat
    16921 cgcctcagca aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac
    16981 ttccccagtt ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag
    17041 taaacgtttg tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt
    17101 aagacgtgga ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta
    17161 gcaatcacaa taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct
    17221 cggatttttc gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat
    17281 aaaccaactc gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg
    17341 ttagcaaaag aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga
    17401 tacgcttttc tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac
    17461 cataattacg attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa
    17521 accccagtgg cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca
    17581 cgacgtccat ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt
    17641 ttatttggtt ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta
    17701 taaacacaga catactttga tagtataacg acaaagtagt gattttgtca atataaaata
    17761 gaagatcgat tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg
    17821 aaaaagttat gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca
    17881 cacgtgtaaa aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta
    17941 ttggtatatc tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt
    18001 tttccacctg tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt
    18061 ttctttttgt cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg
    18121 gaaaactatt gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga
    18181 ggccttatat ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg
    18241 atttatcgcc tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc
    18301 aaaaccagct cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc
    18361 tggcgacaaa aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct
    18421 ttattactgg cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg
    18481 tggcacctag tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg
    18541 tcattggtaa aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag
    18601 gttctattta taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga
    18661 tgcatgcaat tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa
    18721 gcagtgaaac ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt
    18781 tccgtcaaga atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag
    18841 aaaaagaagc tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga
    18901 aacaaatcgt actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc
    18961 ttgtttctcg ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa
    19021 gaacagcgat tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac
    19081 taagctacat cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc
    19141 gagtgcaagc ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg
    19201 cactgaaaac gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag
    19261 aagaagtggc aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca
    19321 aatctattgt tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt
    19381 ctttaccgaa aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc
    19441 atgaaaaaat ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa
    19501 tcaaaaactt tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata
    19561 aaagaaaaaa acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct
    19621 cttacgtttt ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt
    19681 aactaataat aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg
    19741 agctagcttt atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat
    19801 ccctattttg acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag
    19861 caacgctgat atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca
    19921 aatcgaatct actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag
    19981 acttcctgaa gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc
    20041 tggaaaagcg aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt
    20101 tgtcgaaagc cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga
    20161 aacagttttt gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat
    20221 tttatctgat aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt
    20281 gattccagta gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat
    20341 cgaattatct cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa
    20401 ccaagtgttg ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta
    20461 tccagatacc aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc
    20521 tagcttttta gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat
    20581 cgttcgttta tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg
    20641 gaaagtagaa gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt
    20701 caatccggat tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa
    20761 atttatctct gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat
    20821 tcagctgatt actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta
    20881 attgaaaaga aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg
    20941 ctttctgttt atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa
    21001 tatatggata aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa
    21061 tgaaaatgag ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt
    21121 gggccaaatg ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt
    21181 ggcagaaaac actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta
    21241 tcccggcatg atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa
    21301 cgacgataat gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga
    21361 acatcgaatt ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga
    21421 atcttttgga aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc
    21481 aggaattgat tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt
    21541 catccactct tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc
    21601 atatcgaaca gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac
    21661 cagaagatct ttctttagta aaaggcagcc cgcagatcag gcggaagttt ttagatatgg
    21721 aattaggcca gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga
    21781 aacaaagaaa ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt
    21841 tggatatttt gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac
    21901 agttcgtcaa gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa
    21961 aagagcagct ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag
    22021 agcaaatcca agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc
    22081 ggggaacaac gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa
    22141 atgtgcagac ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag
    22201 cagaaatcga tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg
    22261 tgatgagcga gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag
    22321 ttcaaacgtt cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac
    22381 ctgagatttt ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga
    22441 aagaagttta gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg
    22501 acttgaagcc gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct
    22561 gcatcacctt gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc
    22621 aacgaaaatc catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg
    22681 ggggatacct gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac
    22741 tgttttacat gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca
    22801 cggagtagga tcttctgttg taaacgcgct ttctacacag cttgatgtca aagtatacaa
    22861 agaaggaaat gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat
    22921 tatcgaacaa acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt
    22981 tacagagaca actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt
    23041 cttgaaccgc gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa
    23101 tgaataccat tatgacggcg ggatcaaaag ttatgtcgaa tacttgaatg caaacaaaac
    23161 cgtgattttt ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt
    23221 ctctatgcaa tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca
    23281 tacgtatgaa ggtggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa
    23341 tgattatgcc cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga
    23401 cgtgcgagaa ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg
    23461 acaaacaaaa acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag
    23521 cgaacatttc atgaaattct tgctggaaaa cccaagtgtg ggccggcaaa ttgttgaaaa
    23581 aggattacta gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg
    23641 taaaggtgct ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga
    23701 tcctgaaaaa tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca
    23761 aggtcgtaat cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga
    23821 aaaagcaagc atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat
    23881 gggtacaggg tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat
    23941 catgactgat gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta
    24001 tcgttacatg cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata
    24061 cggggtaaaa caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga
    24121 gttgaaacaa gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa
    24181 aggacttgga gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg
    24241 gatgatggcc cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt
    24301 gatgggggat cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa
    24361 gaacttagat atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca
    24421 tccatgatgt caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg
    24481 ttattgtcgc acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta
    24541 ttttatacgg catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca
    24601 gaatcgtcgg agatgtcatg ggtaaatacc atcctcatgg agacattgcg atctatgagt
    24661 caatggtacg gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa
    24721 actttggttc tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga
    24781 gcaaaatcgc tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca
    24841 actatgatga tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag
    24901 tgaacggaac aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt
    24961 ctgaggtagt agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat
    25021 tgatggaagt attacctgga ccagattttc caacaggtgg tttggttatg gggaaatcag
    25081 gaatccgaag agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga
    25141 ttactgagat gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca
    25201 ataaagcaaa attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga
    25261 tcacagattt gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc
    25321 gtgacgccag cgcatccgtt atcttgaata acttatataa attgacatcc ttgcaaaatt
    25381 catttggatt caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac
    25441 aaatccttga aaactatatc gaacatcaac gcgaagtcat cacacgacgg acggtctttg
    25501 aaaaagaaaa agcagaagca agagcacaca tcttagaagg tttgcgtatt gctttggatc
    25561 atatcgatga aatcattgcg attatccgcg ggtctaaatc agatgacgag gcaaaagcaa
    25621 caatgatcga acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt
    25681 tacgccgatt gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa
    25741 aactgattga agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca
    25801 agaatgaatt agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag
    25861 tcggtgaggt cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta
    25921 cattgacaaa caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa
    25981 ggggtggacg tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg
    26041 tttcttgttc tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg
    26101 caaaaggata cgaaattcca gaatacgggc gtacagccaa aggaatccca atcatcaatt
    26161 tactaggtat cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag
    26221 aagaaggcca ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca
    26281 ctgcttttgc taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg
    26341 agctcgtcaa tgtgctactg acagatggtc aatctaatat cattattgga acacataacg
    26401 gctactctgt cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg
    26461 tcagagggat acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaaa
    26521 atcaagaagt attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat
    26581 atccagtgaa aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg
    26641 gtccactagc tggattgacc actgtttctg gagatgaaga catcatgctg attacagata
    26701 aaggtgtcat tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag
    26761 gcgtacgttt gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac
    26821 cagaagctgc agaacaaaca gttgaagaac aaggatcaga agaataacca tcaagactaa
    26881 gtgtgtaagc tagcaagaat aaccgattaa aaaatctggt tattcctgct ggcttttttg
    26941 attagttgaa tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa
    27001 atagagtgta taattagagc ttgtgagtag tctaattaat tagataactc tccttgctct
    27061 taggaaccta agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat
    27121 gaaatcatgt acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa
    27181 cgtttcgatg caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa
    27241 aaacgccgtt tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa
    27301 gtatcttctc catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac
    27361 gacgatatca ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa
    27421 caatagaaaa cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg
    27481 tctgacgaaa gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac
    27541 tttggctgtg aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa
    27601 ctgtgtgatt tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact
    27661 tttaggtgtt actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt
    27721 atacgtgaca gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga
    27781 aaatcgccaa caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg
    27841 aaacaataac tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga
    27901 tacttctgac ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt
    27961 ctaaacgaat ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac
    28021 gtcgtaaagt agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg
    28081 aattactaaa aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta
    28141 caggcgctaa aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct
    28201 tgctaccttt cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga
    28261 tcagctattc aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat
    28321 gacgaaagtt atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa
    28381 aacaatcatt tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg
    28441 gtagtcttaa ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta
    28501 tggggtgttc gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa
    28561 atccagcagg cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct
    28621 gtaggagtga ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca
    28681 ttgaaaatca aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact
    28741 gcaaaagaaa aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg
    28801 gatacagtaa agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc
    28861 catcagacga acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc
    28921 attgataaat tagatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt
    28981 tctgactgga tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc
    29041 ttcgtagcac agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat
    29101 cgctttcgag cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct
    29161 tatggctcaa gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct
    29221 ttagtccgtg gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt
    29281 tattatgggg ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg
    29341 agtaccgctt tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag
    29401 tttccggata tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac
    29461 cagaaaaaag cttacatcgt tatcaatgag aacgagatca ttccagatgt agaacggtgt
    29521 cttgaagaaa tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc
    29581 atagagctga aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta
    29641 tcgatctctc agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt
    29701 cgtggggaag agtttccaag taaacctctg ctgacttata ttgaatcttc agcttcttca
    29761 gcttcagagc tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat
    29821 cgttttgagg ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc
    29881 cgtacaactg cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca
    29941 tccttagtac aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg
    30001 attgctggaa gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa
    30061 gagtataata gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc
    30121 aatgcagctt tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt
    30181 accggcacga tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca
    30241 aacgcagcta ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct
    30301 gcaatcaaag aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt
    30361 ttttacaaga tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt
    30421 atgcacaaaa ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca
    30481 gtcagttagc aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag
    30541 ctaaaaagtt aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag
    30601 caggggaaga cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa
    30661 acaaacaata caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt
    30721 cactaggcta cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag
    30781 ttcatgtcgt acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag
    30841 aaggaaaaaa gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag
    30901 ctgcaaatcg atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa
    30961 gaaaacgagt ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac
    31021 ttttctctca ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg
    31081 aagtgtggca ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct
    31141 ctgtttttct tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact
    31201 tttatcgtcg tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg
    31261 aagcgattga cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg
    31321 ccggtggttt aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg
    31381 tctactatgc gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa
    31441 caggaatcgt tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg
    31501 ccgaacgcag tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg
    31561 ctgaagtact gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa
    31621 tcacaggtct accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg
    31681 aagaactaat cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata
    31741 ttgctcaaaa tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg
    31801 gggcagaatc tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt
    31861 tacgtacagg tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc
    31921 tttctcgagc aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg
    31981 cgaaatgtcg gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt
    32041 tgcagttgat tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc
    32101 gccaactgaa aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat
    32161 ctcgtggagt agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg
    32221 gatcaattga acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa
    32281 gagaaggcgg agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca
    32341 tcatcgagaa gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat
    32401 acaataagtt ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt
    32461 gagcaatccc gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt
    32521 acttaattta aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt
    32581 ccgaataaac ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat
    32641 gaggaactat tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc
    32701 tcttttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa
    32761 acatgagaag cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac
    32821 aacctctttc ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg
    32881 aaaaatatga gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt
    32941 tcacaacctc tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt
    33001 cacaaaaatt tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt
    33061 ctgttacaac ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt
    33121 tttataaaat aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata
    33181 caatggttca ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt
    33241 aggtacgcaa tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc
    33301 tgaagtgatt gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg
    33361 tgtgacttat aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt
    33421 gatcggaaac ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca
    33481 tgaaaaaggt gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc
    33541 ttatcatatc aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac
    33601 aacgattaaa ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat
    33661 tgcagactta ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa
    33721 aaaccgtcaa tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga
    33781 agaatattat gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt
    33841 gaatgatgct ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct
    33901 ggatatcgac cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt
    33961 aacaatcgga agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc
    34021 ctatacgtca cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga
    34081 acaaatccgc gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg
    34141 ctggtttgat tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc
    34201 tttaaactcg atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga
    34261 attagatggc gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa
    34321 accaatctat gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc
    34381 agaactgcca gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg
    34441 tatctcaaca ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg
    34501 ggcgcaaatc taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg
    34561 aagtgtttat ctatacgata ataataactt gaagaggtag tgaaagagac gtttagcctt
    34621 attgccgata ggctgtctct tgaacactgt ttattcagaa taagggactg tgacaaaaac
    34681 cgtgtcacag tccctttttc cgaa
//
LOCUS       DAZKMK010000019        32533 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000019 DAZKMK010000000
VERSION     DAZKMK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.019"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8V1_000958"
     CDS             131..505
                     /locus_tag="ACG8V1_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0329072.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8V1_000959"
     CDS             complement(619..1248)
                     /locus_tag="ACG8V1_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0329073.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8V1_000960"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8V1_000960"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG8V1_000961"
     tRNA            1668..1741
                     /locus_tag="ACG8V1_000961"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACG8V1_000962"
     CDS             complement(1879..2043)
                     /locus_tag="ACG8V1_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329074.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACG8V1_000963"
     CDS             2338..3666
                     /locus_tag="ACG8V1_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0329075.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACG8V1_000964"
     CDS             3868..4701
                     /locus_tag="ACG8V1_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0329076.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACG8V1_000965"
     CDS             4717..5454
                     /locus_tag="ACG8V1_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0329077.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACG8V1_000966"
     CDS             complement(5609..6577)
                     /locus_tag="ACG8V1_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0329078.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACG8V1_000967"
     CDS             6809..7642
                     /locus_tag="ACG8V1_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0329079.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACG8V1_000968"
     CDS             7676..8515
                     /locus_tag="ACG8V1_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0329080.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACG8V1_000969"
     CDS             8541..9101
                     /locus_tag="ACG8V1_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0329081.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACG8V1_000970"
     CDS             9300..11021
                     /locus_tag="ACG8V1_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0329082.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACG8V1_000971"
     CDS             11186..12328
                     /locus_tag="ACG8V1_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0329083.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACG8V1_000972"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACG8V1_000972"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0329084.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="ACG8V1_000973"
     CDS             14128..14775
                     /locus_tag="ACG8V1_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0329085.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="ACG8V1_000974"
     CDS             15168..17813
                     /locus_tag="ACG8V1_000974"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0329086.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="ACG8V1_000975"
     CDS             18123..19436
                     /locus_tag="ACG8V1_000975"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0329087.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="ACG8V1_000976"
     CDS             19426..20079
                     /locus_tag="ACG8V1_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0329088.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /gene="radC"
                     /locus_tag="ACG8V1_000977"
     CDS             20134..20826
                     /gene="radC"
                     /locus_tag="ACG8V1_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0329089.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="ACG8V1_000978"
     CDS             20976..21176
                     /locus_tag="ACG8V1_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0329090.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="ACG8V1_000979"
     CDS             complement(21430..23109)
                     /locus_tag="ACG8V1_000979"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0329091.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="ACG8V1_000980"
     CDS             complement(23111..23323)
                     /locus_tag="ACG8V1_000980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0329092.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="ACG8V1_000981"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="ACG8V1_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0329093.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="ACG8V1_000982"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="ACG8V1_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0329094.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="ACG8V1_000983"
     CDS             27298..27495
                     /locus_tag="ACG8V1_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329095.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="ACG8V1_000984"
     CDS             27648..28133
                     /locus_tag="ACG8V1_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0329096.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="ACG8V1_000985"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="ACG8V1_000985"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0329097.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="ACG8V1_000986"
     CDS             29010..29537
                     /locus_tag="ACG8V1_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329098.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="ACG8V1_000987"
     CDS             29628..30365
                     /locus_tag="ACG8V1_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0329099.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="ACG8V1_000988"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="ACG8V1_000988"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0329100.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="ACG8V1_000989"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="ACG8V1_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0329101.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5521 c         6874 g         9366 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgtt gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DAZKMK010000020        32497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000020 DAZKMK010000000
VERSION     DAZKMK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.020"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG8V1_000990"
     CDS             complement(<1..563)
                     /locus_tag="ACG8V1_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0329102.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG8V1_000991"
     CDS             complement(750..1400)
                     /locus_tag="ACG8V1_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329103.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8V1_000992"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8V1_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0329104.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8V1_000993"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8V1_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0329105.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8V1_000994"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8V1_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0329106.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG8V1_000995"
     CDS             complement(4926..5657)
                     /locus_tag="ACG8V1_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0329107.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG8V1_000996"
     CDS             complement(5661..6926)
                     /locus_tag="ACG8V1_000996"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0329108.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8V1_000997"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8V1_000997"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0329109.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8V1_000998"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8V1_000998"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0329110.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG8V1_000999"
     CDS             12268..13770
                     /locus_tag="ACG8V1_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0329111.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG8V1_001000"
     CDS             13772..14509
                     /locus_tag="ACG8V1_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329112.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG8V1_001001"
     CDS             complement(14680..15873)
                     /locus_tag="ACG8V1_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0329113.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG8V1_001002"
     CDS             complement(15952..16158)
                     /locus_tag="ACG8V1_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0329114.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG8V1_001003"
     CDS             complement(16151..17293)
                     /locus_tag="ACG8V1_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0329115.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG8V1_001004"
     CDS             17805..18440
                     /locus_tag="ACG8V1_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0329116.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG8V1_001005"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG8V1_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG8V1_001006"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG8V1_001006"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8V1_001007"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8V1_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0329117.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="ACG8V1_001008"
     CDS             21086..21253
                     /locus_tag="ACG8V1_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0329118.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="ACG8V1_001009"
     CDS             complement(21313..21666)
                     /locus_tag="ACG8V1_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329119.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="ACG8V1_001010"
     CDS             22170..22589
                     /locus_tag="ACG8V1_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0329120.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="ACG8V1_001011"
     CDS             22615..22821
                     /locus_tag="ACG8V1_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329121.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="ACG8V1_001012"
     CDS             complement(23049..23222)
                     /locus_tag="ACG8V1_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329122.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23234..23863
                     /locus_tag="ACG8V1_001013"
     CDS             23234..23863
                     /locus_tag="ACG8V1_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329123.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACG8V1_001014"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACG8V1_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0329124.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACG8V1_001015"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACG8V1_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0329125.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACG8V1_001016"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACG8V1_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0329126.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACG8V1_001017"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACG8V1_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0329127.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACG8V1_001018"
                     /pseudo
     CDS             complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACG8V1_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(30257..30484)
                     /locus_tag="ACG8V1_001019"
     CDS             complement(30257..30484)
                     /locus_tag="ACG8V1_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0329128.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31575
                     /locus_tag="ACG8V1_001020"
                     /pseudo
     CDS             30968..31575
                     /locus_tag="ACG8V1_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            31748..32461
                     /locus_tag="ACG8V1_001021"
     CDS             31748..32461
                     /locus_tag="ACG8V1_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329129.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9520 a         6618 c         5736 g        10623 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagc
//
LOCUS       DAZKMK010000021        31057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000021 DAZKMK010000000
VERSION     DAZKMK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.021"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACG8V1_001022"
     CDS             complement(159..1469)
                     /locus_tag="ACG8V1_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0329130.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2839)
                     /locus_tag="ACG8V1_001023"
     CDS             complement(1481..2839)
                     /locus_tag="ACG8V1_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0329131.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3041..4609)
                     /locus_tag="ACG8V1_001024"
     CDS             complement(3041..4609)
                     /locus_tag="ACG8V1_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0329132.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..5023)
                     /locus_tag="ACG8V1_001025"
     CDS             complement(4613..5023)
                     /locus_tag="ACG8V1_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0329133.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5020..5943)
                     /locus_tag="ACG8V1_001026"
     CDS             complement(5020..5943)
                     /locus_tag="ACG8V1_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0329134.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5940..6818)
                     /locus_tag="ACG8V1_001027"
     CDS             complement(5940..6818)
                     /locus_tag="ACG8V1_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0329135.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6805..8142)
                     /locus_tag="ACG8V1_001028"
     CDS             complement(6805..8142)
                     /locus_tag="ACG8V1_001028"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0329136.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8145..9248)
                     /locus_tag="ACG8V1_001029"
     CDS             complement(8145..9248)
                     /locus_tag="ACG8V1_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0329137.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9322..10830)
                     /locus_tag="ACG8V1_001030"
     CDS             complement(9322..10830)
                     /locus_tag="ACG8V1_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0329138.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10796..11488)
                     /locus_tag="ACG8V1_001031"
     CDS             complement(10796..11488)
                     /locus_tag="ACG8V1_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0329139.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11510..12484)
                     /locus_tag="ACG8V1_001032"
     CDS             complement(11510..12484)
                     /locus_tag="ACG8V1_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0329140.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12784..13656
                     /locus_tag="ACG8V1_001033"
     CDS             12784..13656
                     /locus_tag="ACG8V1_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329141.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14114..16312)
                     /locus_tag="ACG8V1_001034"
     CDS             complement(14114..16312)
                     /locus_tag="ACG8V1_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329142.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16544..17536
                     /locus_tag="ACG8V1_001035"
     CDS             16544..17536
                     /locus_tag="ACG8V1_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0329143.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17596..19017
                     /locus_tag="ACG8V1_001036"
     CDS             17596..19017
                     /locus_tag="ACG8V1_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0329144.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19010..20509
                     /locus_tag="ACG8V1_001037"
     CDS             19010..20509
                     /locus_tag="ACG8V1_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0329145.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20548..21942
                     /gene="uxaC"
                     /locus_tag="ACG8V1_001038"
     CDS             20548..21942
                     /gene="uxaC"
                     /locus_tag="ACG8V1_001038"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0329146.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22101..22784
                     /locus_tag="ACG8V1_001039"
     CDS             22101..22784
                     /locus_tag="ACG8V1_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0329147.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22786..23622
                     /locus_tag="ACG8V1_001040"
     CDS             22786..23622
                     /locus_tag="ACG8V1_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0329148.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23633..25387
                     /locus_tag="ACG8V1_001041"
     CDS             23633..25387
                     /locus_tag="ACG8V1_001041"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329149.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25663..26004)
                     /locus_tag="ACG8V1_001042"
     CDS             complement(25663..26004)
                     /locus_tag="ACG8V1_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0329150.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26072..28243)
                     /locus_tag="ACG8V1_001043"
     CDS             complement(26072..28243)
                     /locus_tag="ACG8V1_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0329151.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28454..29311
                     /locus_tag="ACG8V1_001044"
     CDS             28454..29311
                     /locus_tag="ACG8V1_001044"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0329152.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29382..30182)
                     /locus_tag="ACG8V1_001045"
     CDS             complement(29382..30182)
                     /locus_tag="ACG8V1_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0329153.1"
                     /translation="MTIKKKLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGID
                     KTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNS
                     CIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAA
                     GPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLL
                     QKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(30179..>31057)
                     /locus_tag="ACG8V1_001046"
     CDS             complement(30179..>31057)
                     /locus_tag="ACG8V1_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0329154.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPL
                     EVFLSYVSIDDLSNLI"
BASE COUNT         9296 a         6392 c         5496 g         9873 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgcgaaataa aaaaatgcaa
     4681 aaggaaacgc aataaactga gtgccaatca acaatatgaa tcctgttata aaatacaaac
     4741 cagtggcagt tgttaagtat tttcctttag cagtcaaaaa caatgctact gctaaaaaag
     4801 caagtgaaat aaacaacaca acggcaaaca gaccagtcat gtcctgtaat aatccaaatg
     4861 tattttctaa agacaaagtt gaatgatcta cagcaagcga ctgataactt tttttaaact
     4921 ctgacgcaga catattactt aaagtaactg ctaacccgcc aaagaaaatc aaagatagac
     4981 tgccaccgac cattcctagc aatagttccc attttctttt catttctgaa ccccctgctt
     5041 ttctaaatgt atccattcag gtcttttgat cgatgtttca taattgttat tacgttcaga
     5101 gaatttgaca gtctgttcat ttttcttgtc atcccaatta tcccaaccag ttgggtgaac
     5161 atgcccacct atcttacacg cttcaaataa gacatttgca tacggacgcc acggtcttcc
     5221 taaatagacg tttctagtat caggttctgc agtcaaggta cagctttcaa aacgataccc
     5281 tgttttttga tttttcggcg tagatgctgc tgtgatgtat ccaggatttt tactggctaa
     5341 gctatggata tgacactgat gaaaaacggc atctgcccca ccaaatatga aatccaccgt
     5401 tccttcaatc cagcaatatt cataggtctg acgacaatac tcaggattgg cagaagccgg
     5461 tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac ggacccgtgc ataaggtatc
     5521 ctgataacca gatagtcgac agttttttaa agatacgcga tctcctgaat tgaataaagc
     5581 aacagcctgt cctacctttt ctcctggacc tgctgtattt tcgatgtgaa gattttctaa
     5641 tacgacattc gatccttcta agaaaaaagt agcacttcgg aaagtcccta actggctacc
     5701 gtcaagatac tcctgttttg caaaacgatt accggagata actactgttc ctgctcccac
     5761 aagtttaaaa ttggataatc tgctttctat atattcatca tatcttcctt cttttactat
     5821 caagaccttt ggatggctat ccggcaggcc tgccaaataa tcgattcctt cttggatagt
     5881 ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt atcagtttgt ctttcatcct
     5941 cataaatgct cccttatgaa ttgtttccaa atatttctca tttcttgtgt ttccatatga
     6001 ccgcctgtta aagaaaaaga agaaaagttt tctggactac ccattgattg atactttttg
     6061 tccagccctt ttctcagctt cagtacgcct tcgtccaagc acatcgtatc gttttttcca
     6121 actaagctaa gacgagggcg cggagcgatt ttttcttgga ttgctaacgt tgtataagct
     6181 gtcaatagat ttggcacata gtaataaaac ccatgatgat ctaggcggcg atgtagcaaa
     6241 agtatttcga tgtccacctg tgccgctaga tcaatacaaa ctttgacacg agaatccaat
     6301 gctgtcaccc accagcttaa taagcctccc atagaaagac caatcgttgc gagccgttga
     6361 gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc tgatcacatc ataaatacgc
     6421 attccccata acgtttttcc tgttaataaa aattgtttga acaactcgct ttcagaaata
     6481 cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa cagcatatcc cattcctgtc
     6541 agtacttctg caaaagatgg agaagctaag taaggagcac catggatcaa ttcacttttc
     6601 ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga ccggcggaaa cggtccttct
     6661 ttttctggat aagcatagag ggctggtact gtttccaatt catttaagta caattgatag
     6721 tattcaagca taaaaccgtt ttgtttttct cggtataatc gttttccact aggggtatgt
     6781 ctgctagatg gagcacctaa aaaatcattc aactgcataa gcaaagtttt tacgaacaac
     6841 cagttcatcc atattttctg attgatttga ttggaatttc tcaatactga tttctttagt
     6901 attttctaat agaaatgctg gtccctcgtg attttcaatc gacatctggt taaaggaaac
     6961 cttacccgca aagccaatat aaaatccttg tctgctcatt tcctctattc ctgtcatcat
     7021 agcaggaaca ccgggaactg catcttgggc catcgagaca tcgatattgt caaatgtgat
     7081 ttcttgtatg aattgttccg ctaatccata aataaaacct gcagaagcat ggacattcct
     7141 tgccgtgata ttcgaaaaat ggatccttct gaatgctggt gtccgttcat ctatcggata
     7201 agcctttttt tcccatacat acggttcttt ccctcgcgga ccacagaaat aataaagatt
     7261 cagtataaat ggacacatca cattatccat gactatattg gatactcgaa tatcttcaac
     7321 gatcccgcct cgacctcgtc tagatttcaa tcgtattcct cgatcagttt cttggaagat
     7381 acaatttgaa atggtaatat tacgaatact accactcatt tcacttccta atacgacacc
     7441 gccatgtcca tggaccatcg tgcaatttgt gatcgtgata ttttcgcaag cgatcctttc
     7501 gtacgtatct tcagtccctg ccttgattgc aatacagtca tctcccacgt caatatggca
     7561 attactaata cgcacatttt tgcatgattc tggatcaatt ccgtctgtat ttggtgaatc
     7621 cgctgggttc aagatcgtca agttatcaaa cgttgcatta ctgcaaagaa tcgggttgat
     7681 cgtccaactt ggtgactgga tcaatttaat atctttcaca gtaattcgat gacagttatg
     7741 gaaactcatc aatttaggac gaggataagc gagattatct ggttcatttc taaaagtatg
     7801 ccaccatttt tttccatttc catctaatgt accgaaacca gtgacagaga tgttctctac
     7861 attttgagca tagatacagg aagcataaac ttttcgatgg actccttccc aacgtgaatg
     7921 aacaacagga taatcttttg gatcgtcaga gaatttcaga accgctcctg cgcttaaatg
     7981 aagttcgaca ttgcttttta aaaataaagc tcctgttaga aattcacctg ctggaacaac
     8041 tacggtcccg cctccgtcac ttgctgcagc atcaatggct tgttgtattg cttcagtatt
     8101 caattcatca attgatgcgc caaatgtcaa aatatcatac atatttataa tgcctccact
     8161 tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat cattacaaat aattggttca
     8221 ctgatgtagt aagcatatga accatctcgc tgatctgctc cgcctaaccc tgctacttga
     8281 cagtttttat tcaagttcag ccagccttct tttgtttcca gaacaaattc atcaagtaat
     8341 ccttgatgtg atttcttcac ttgtggaagc cattcttctt tgttcagcag tcccaaacgt
     8401 attcctttag ccattgcgta aacaaacata ctactgccgg aagcttccag ataatttcct
     8461 ttccgttttg tttctgttgt tacttgatac catgtcccgc tgttttcttc ttgatatgga
     8521 agtaatgcag ccagtgtctt ttctagaatt tctttcagca aagaacaatc tgtttcttca
     8581 tgaagtaatt cataggtatc tgctgcagcc atcaaatacc agccaatcga tcggctccaa
     8641 aaatgatggg atagacctgt ttttggatca gcccatggtt ggaccttctt ttcatcccat
     8701 gcatgaatca gcaaatttgt ttttggatcg aatagatgtt tataactgat ttcaaactgt
     8761 ctcaaaacat cttggtaatc tttttcttca ctaaaattct ggataaattc agcataaaaa
     8821 ggagaaccca tatataagcc atccaaccaa atttgataag gataaatttc tttatgccaa
     8881 aaagctcctt cggatgtgcg tggatggttt gccaactgct gggaaaggag ttctgctgct
     8941 tttttatatt tttcttcgcc tgtttctttg tacaataaaa agaaaagctt tcctgtattt
     9001 acatggtcga tattgtattc ttcaacgctg tagccacgga tcgttccatc ttcttgcacg
     9061 aaatagtcga tattgtcttt tatgaaatca aaataaattt tttcttgggt tcgttcccaa
     9121 agtaattgga accctttaag tacgacgcca tagtcataag accattttcc tttataccac
     9181 ttttcttcat ataagcgagg tgttctttcc atgatacttt ttgctaattt ctttgaccag
     9241 ttttccatta tttgctctcc tttaaaaatt aaaataggat ttgggacaaa taccaagaag
     9301 tatttgtccc aatcgtctat attactgtgc cgctgctttg tatgcttcct cgtactcttg
     9361 tgtgattcga tcgcctcctt gtgctttcca gtcttctaca gcttttttga atccatcttc
     9421 gtcaagctgt cccatgatgt attgcaccgt agcatcaatg acgatttttt caatttctga
     9481 tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa ggatccatta cagcaaattc
     9541 ttcattttct ttgatcaatt gattagagaa ttctttttca gggtctgtat ctttgaaaga
     9601 gaacgtcact tcactaggac gagagcttga taacggttgc acctcttgtt gccaaagatc
     9661 catatcgata aattcgatag aaccatcatc attcaattca taatgcgttc cttcgatacc
     9721 gccagtcatc aatttataca catcttcttc taaaagatca tcaacaaatt gaagtaattt
     9781 cttcaattgt gcttcgtctt ttacttgaga tttgggaaat gctagcaagc cgccaatacc
     9841 atttccttct gaccaaacat gataaccgcc gccttcacca tctgagatct tgtttacagg
     9901 cactaattcc atatcatctt gaataccttg acccatcgtc ttcagattct tgatatccac
     9961 cattccagtg tagattccag ttcttccttg tgcaaattgt tcttgctgat cagtcttttg
    10021 ggtaactgca aagtcttgtg ccaaatagcc attctcaaat agatctcttg aaaaattcaa
    10081 agctttcata tactcttctg tatcaaattc tggagtgaat ttgccattat cgtctacagc
    10141 ccagccattt ggtgctccaa aataagaagt gaaaagttta aagctggtat aacgaatatc
    10201 actaggcgca cgatcgccaa aaccaactgt gtcgtccaca ccatttccat caggatcgtt
    10261 ttctgtaaat tgtttagcta cttcataaag ctcatccatg gtagttggaa catctagtcc
    10321 tagattatcc aaccagtctt ttcgaatgac taagccagca cgagcgtagt ctttttgtat
    10381 cggtacgccg tacaaggcac cgtcgatact cgcagcttcg attctatctt cagaaatttg
    10441 acttaaattt ttatagtctt ttaaatattc tgatacatcc cagaatacgc ctgattttaa
    10501 cgcattgcgg acagaagagt ttttcaacat cgcaatttga agtgtaacga tatctcctag
    10561 ttcatcagaa gccaatgcag ttgttaatct ttcttctttt gaagcatcag gaatccagtt
    10621 gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc acaatcgtgt ctgtaggcgg
    10681 tgaagcatga tgcaaaatat ttaaccaagt gattttagct tttccatctt ctgtctctga
    10741 agaactgtta cttgaactgc tgccgcatgc ggcaagtgct agaattcctg caaaactaag
    10801 acctaatttt acgagcgttg cttttttcat aaattttctc cttcactgtt ttaataatat
    10861 ttttgttttc ttctgttttt tctgtatatg agttcttcca ttccaaatgg caaaatatag
    10921 aatttgctgt ccacgtgact gcggaaaaga agagactgcc accgataaag gttaaaacac
    10981 ctggcattgt ttggatgatc atcagataaa tccctaccat acttaatatc aacactaatg
    11041 agtaatgaag atgtgagaat ccgaataaaa atgccatttt cattttgaaa aaatatcctt
    11101 tccaatcata atgagccatg attgggaaaa tatagagtgc tgagagcaca tatatgaacg
    11161 cagcaatata caaagcgatc ctaagatacc aattgtgtat gaaagaaaga tccacaagta
    11221 aaacaatccc gatggcgcca tatatccagc tcatcagcaa ggtttcacga tagttttctt
    11281 taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc ttttgagcgc agccattttc
    11341 taatcactgc aaccatcgca tacgatgcag gaccgaaagt aaaaactccc aaacctgcga
    11401 tgactaaacc gccccacaga aaattcaaat aaaccaactg taaaatacga tttagccttc
    11461 cgtttatttt atctaatgac tgcagcatat ctttccctct ctttctatct tagtaaacgc
    11521 cgtcttctcc tagctttttg gcaatcttat tagctaaaat taccataaac aatcctacga
    11581 cccctttaaa caatcccatt gctgtactga aacttaactg tccattcttc aatccagctg
    11641 tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt caatgaattc aataacaagt
    11701 acatatgctc gaaccctaag tctaatgtct gaccgatttt tagaatcagc aaggtaatga
    11761 ttactggacg aattgccggc aatgtaacat gccatgtttt gcgtaagcgt cctgctccat
    11821 ccatttctgc agcttcatac aactgtgtat cgactgcagt tatcgctgaa aggtagatga
    11881 tcgtagacca gcccaactct ttccagatca cttgtccgat atatactgtg cgtgtccatt
    11941 caggggatgt tagaaaactg attttttcaa atcccattgt tgcaaggaat tcattcaata
    12001 cccctccgtc aacattcaaa aatacataag taatcgaaac aatgattacc caagacatga
    12061 agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa tcgtttgttc gttacttcat
    12121 ttaatagtaa agacaaaatg attggcatcg gaaaaaacac gaagatattt aatccgaaca
    12181 agatcaacgt atttcttaat aatgtaaaga atgtcggttc tgtaaacaaa cgaatgaaat
    12241 gtttgaaacc gacccaagga cttcccatta ttcctaaaaa tggttgataa tcttgaaaag
    12301 caataacgag tccccccata ggaagatacc taaaaatcaa gaaataaagg actcccggca
    12361 gaatcatcac atataaaaat ttattcgttt tgattcctgc aattattttt tttcgtcttc
    12421 tgagcttttc ttcttttttc gatagcgtca gttgaaatgg ttcttgtttc tttacacttt
    12481 ccataagccc ctccttagtt ttgtgaatgt ttgctgctta tgacttcatg atagataact
    12541 atctgaatta cgtatataat catttttacc gtaagtaaaa acgcttacat agcaatgatt
    12601 tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa taaactacgc atagcaaacc
    12661 ttctattttt ttctatctga cacttagcat aggaaatgat tatatacgta atctttcacc
    12721 gatgataaat tgaacatata gaaacagaaa catttaattt attttgagga ggagcctatc
    12781 tatatgaaaa agaaagtctc tataggagaa agaagtttta cacttttcaa caatacgttt
    12841 ctaattttgc tagctttgat ctgtatcgtt ccttttttaa atatcatcgc tacatctttt
    12901 gcttctacac aagaagtcgt cgcgaaaaag tttatccttt ttcctactac cttttcttta
    12961 gacgcgtatc gctacattct ttctacgccg accattttta gagcacttgc tgtttcgatt
    13021 ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa cttcactgat ggcctacggt
    13081 ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact ttcttgttgt attttctatg
    13141 ttgttcagcg gggggatgat tccaacgttc ttagttgttc gttctctagg gctggtcaat
    13201 tcttactggg ccatgatcct gcctgtggct gttaatgcaa tgaatatgat cattatgaga
    13261 aactttttcc aagccttgcc ggatagtttg gaagaatcag caaaaatgga tggttgcaca
    13321 gattttggtg tatttttcaa aattatgctg ccattagcgt taccgtctat tgctactatc
    13381 tcgctttttt atgctgttac ttactggaac acttatatga ctgctatttt gtatatcaac
    13441 gattcatcta aatggccgat acagattctt ttacgccaaa tcgtcattgt atcaagtggg
    13501 atgcaagcag aaagtagtgc cgttgatgtg attccaccag cacaaacagt taaaatggct
    13561 gttatcgtta ttgcaacagt gcctatgctg attgtttatc cttttgttca gaaatatttt
    13621 gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat gtactgaacg caaaatggta
    13681 gttgtgtcag aagtggtcag cttcaagaaa taagaaggca tttacgaaaa ttgcttctta
    13741 aatttttgtg aaatttcggc ttatttttcg aagaatctac tcctggaaca ccgtgtatca
    13801 gaggttgtga aagagacgtt cagccttgag cgatgataaa aactctcaca aacattgcga
    13861 cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa tagcttccca tatttcacaa
    13921 aattatgcct tattgtcgat agcctgtctc ttgaacgcca tttattcgta aaaaagaact
    13981 gtgacaagac ttttgtcaca gttctttttg cgtgcttagc cttgaaattt aactatccat
    14041 aaatacaaat actagggtta ttctaacttc ttttattgct tgaatcagtc cacaagaaaa
    14101 acagcctctt gattcacaaa tatttttttc gatattctcc cggcgtaatc gattccttct
    14161 ttttaaagaa acgaataaaa ttttgcggat ttcgatattg caatctttct gcaatctctt
    14221 ttacggagag gttagtctct ttcaaccatt tttttgctac ctctaaacga tgattttgaa
    14281 caaaatcacc aaatttttct cctgtttcct ttttaaatac attacttaaa tagttagggt
    14341 tataatgcaa cctatcagcg atcacatcta aagacagatc acgatcatat tctgtgtaga
    14401 cgatatgcat aatattctcc gacaaatttt taaattcatg atcagtgatt gcttgagagc
    14461 attctgtgat tggcagaact aattgttcta aaacaagttt ttctagttct ttgggatgat
    14521 aattgttcag tgcttttaca taaatttgtt tcatgttatc aaagtttttt gaatctactc
    14581 ccaataactg tcctaattga acgagttcat tgatcaagcg gaccgaaact acttcccgac
    14641 tcaaaggatt tttattttgt gcaaacaatg catcaatcaa ttcatgtact aaattcttca
    14701 cttcctgatt tccagaacga atggcttcaa acaatcgatt ttgttcttct atcggatatc
    14761 taataagtgt tttttcatga gcagcaggaa cgatttcctg ataaaaaata attgaacttg
    14821 gaccagtatt tacacgataa tacagggctt cttttgcccg atcaaccgat tgcttgcttt
    14881 cattcagcgt ttcgaaccgg ttactgatgc ctatactgat agttaaattc aagtattctt
    14941 ttacacgttt ttgaatgagt cggcaatact ccatgacttt tttgcttgct tctgcatcat
    15001 ctttatccat tcgataaata gtggcttgca tttcttcatt caaaacgatc ggcagcattc
    15061 gattttcctt tggcacaatt tccgtgatca tgttgttgat cgctaaaagt agcaaatcac
    15121 gctccccacc gtgactatct tctaacagat cgatttgaac caaagctgta taatagcagt
    15181 cattttgaga actataacca aattgctgca accgctgatc tagttctcgc ctttctactc
    15241 gatttctaaa taaactaagt acaaatagcg tttccagctg tggcttttgg gtaactaagt
    15301 gtgcacttaa cgcttctttt tctcctatga tccgactgac tgattgagcg accaaatcta
    15361 gttctttccc actgaatcct tttgatttaa gattcagacg ttcctgtatt tgagaaatcg
    15421 gacgggcaaa tctgtccgaa aataagtagg aaagaagacc aatgagtcct accagtgtta
    15481 aagacactgc aactagcccg attctcagat tgcggatcat tgcgccaatt gcactagagt
    15541 caacttctaa tctgtaaatc caattattat aatctgattt cagtactata tacgttttat
    15601 ctttttctct tatcactttc agctgattgt aatctagatc cacgtccgtt aatacagttg
    15661 gttgctcttt attttttact gtctgagtag taaaaaggtt gccaacttcg ttttcaatag
    15721 aaagtatgtc ttctccactt tcaccaacaa cttgttctat tgtatgtttc tctatcgtag
    15781 caattcccaa tgcatatttt tctttttcaa acattggcaa agaaatcacc atttcgattc
    15841 ctttgttttt cttttgccaa aataaattat ttttgttatt tatgtacttc tgttgatact
    15901 tttcaatttc ctcttcggtc agttgactca acgatccatt gattactccc catttaccag
    15961 ccaaactgac taaatcataa ttattatcat ccataacaat cgtttctata aaatttagtt
    16021 ctttcctgat ttcagaataa gtggagtaat ctgtatatgt cagcggatgc ttgaatatgt
    16081 ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata ttcaatcgtt ctaattttcc
    16141 attcaacgtt agttttaact tgcctgagca aattcttttt gtctgttaaa taactcgatt
    16201 cgatcgattc ataggtattc tggtaaatga ataaactaaa tcctatgaca agtacggctc
    16261 ctaataaaaa catcggtaaa aatattttat aaaatactgt acttttcttc attttcttcc
    16321 tctcctcctt ttaaaataaa aggctttcat ttttctgtaa aattgatact catctaatta
    16381 aatgagcatt ccccgctttc aagaatagca taattaaaaa taaaaatcca agtaattttt
    16441 aacgtgtgca atcttttcat atcaaagatc tattgactta tcgtagttat cgtaatatga
    16501 tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa cgatcaaaga
    16561 tgtcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga ataacagcag
    16621 tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa tgaattattc
    16681 gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg gactattttt
    16741 ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg caggcatcca
    16801 ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg ttgatttaac
    16861 agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg atgatgatca
    16921 gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca atcgtcacat
    16981 caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta tgcaggcagt
    17041 agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa agaaaaattt
    17101 tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag cgagcatccc
    17161 tgtcaatcca gcctattttt taacaggaga ttacagctta gaaagcggcc tccaaaacat
    17221 ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca acgacgacat
    17281 agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc agaacatttc
    17341 tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc ttaccaccgt
    17401 acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag atttgatcga
    17461 aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc ttcgtgagtc
    17521 gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg tgcataacac
    17581 agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa cgaagcacct
    17641 gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga ttggcagatt
    17701 cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca acctttagaa
    17761 caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat tttacaagga
    17821 ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga acaaatgatc
    17881 aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga cttggcctat
    17941 atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa tttaacagat
    18001 actcccccac agagcctccc gggcaaactg acagcctttt tataccgacg tttcaaatta
    18061 aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg cgaacgtcta
    18121 aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt tgttgagtgg
    18181 ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc aggttatcct
    18241 cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt gatggtcaaa
    18301 gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa tcaactacca
    18361 ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta tcgtgaacgt
    18421 aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt gttggcggaa
    18481 ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt tgatcagtta
    18541 ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc catttatgct
    18601 gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga agcgatttca
    18661 ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa gtatatcgaa
    18721 gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact tcttatgtat
    18781 cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt tactgatgct
    18841 tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct ttggggagaa
    18901 gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc aaagatcgat
    18961 caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca tgcttgaaac
    19021 cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat taacccctat
    19081 tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg aagatatccc
    19141 gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga tcaaatatgg
    19201 ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc acactcataa
    19261 tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg tccatccaag
    19321 tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg gaaaagtagg
    19381 gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca ttgctgaact
    19441 gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc atattacgat
    19501 tttaaagcat ccttatggct gttcgcagct tggaaaagat catcaaaata ctcgagaaat
    19561 tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg gattaggttg
    19621 tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg aagaacgagt
    19681 gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg ctctattaga
    19741 agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct ctaagttgaa
    19801 tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta atcctttact
    19861 tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa cagaagtgcc
    19921 agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag aagtttttga
    19981 ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg gggaaccagt
    20041 ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag aagataagtc
    20101 tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac aatatggcga
    20161 aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc ttgtagctgc
    20221 ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa gagggactcc
    20281 gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt ttgaacgcaa
    20341 aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg aagtaatctt
    20401 agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga aaaatgaaca
    20461 aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat tttcataaaa
    20521 cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt attaaaagac
    20581 gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat tgattatcac
    20641 tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct gacagaagct
    20701 tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt gcctgaagaa
    20761 aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca aaccgtacca
    20821 aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg tttcttcggt
    20881 attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc aaatgaaatt
    20941 ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt agaagtgatt
    21001 tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc tcatgaagaa
    21061 ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat tgaaaaagaa
    21121 ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat caccacctat
    21181 caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca tggatgtcgt
    21241 gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac agtattagaa
    21301 gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat cgatgcttat
    21361 cgtacagaaa cactgataca tttgacccac ttctatcatt ctcataattg gacgatgcag
    21421 ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt agggcccgat
    21481 accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa attattgaac
    21541 cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa tccgaatgac
    21601 tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg aaaactgcag
    21661 ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca attgacacaa
    21721 tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc aagaagtttc
    21781 ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt aggcgaaatg
    21841 gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt tcaagcgatt
    21901 agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa tagtaacagt
    21961 aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt ttagagaaga
    22021 agacggacca aatttgctcc gtcttcttct cttgctgttc tgcttcattg cctataaacc
    22081 tttatgaaag aagagataac atgcctacta tccatattgc cggtgattct actgcggcag
    22141 aaaaagatga aaacaagcgt cccgagacag gttggggaga aaaaatctct gcttattttt
    22201 cacccgagat ccagatcaat aaccaagcaa agaatggacg cagcaccaaa tcatttatcc
    22261 aggaaggaag gttagcccgt ctagcttcac agtttcagcc gaatgatttc ttatttgtac
    22321 aatttggaca taacgaccag aaaatccaag aagaaagagg tacgcttccc ttcggtagct
    22381 atttaaaaaa tctgcagata ttttttgaat gcgcaaaaaa agcgaacgtc caaatggtct
    22441 tactgacatc tgtgacgcga cgagattact tagaaaacgg aacattgaat ccagatatac
    22501 ttggagacta tccaaaagcg atgcgtgcat ttgctgagaa acaccatatt cctcttttag
    22561 atgtgttctc aagatcacaa gaattgttcc agaccttctc aaaagaagag acaaaaaaat
    22621 tctacctgca tcttatacct gatacttgta aaaattaccc agaaggacta aaagacaata
    22681 cccactttag tccagaaggc gctgataaag ttgcccgaat aattgttgaa ttgataaaag
    22741 aatcccgtct gcctcttgct aattatttac aaaaaggagt ttgagatgtt tccattaaat
    22801 ttaggcatcc gtgcccacga tttagatgtt cgaaatcgtg aagaaatcgt tcaaaaagta
    22861 tcacagcttc acttgtccca tatccaattt gcaccacaaa aagcatttcc tgaacaactt
    22921 aaattaactg acatgacact tgggagtgct gcctattttg gtgaatactt ccaaaaaaac
    22981 aatatcgaac tttccatttt aggatgctat atcaacctat ccagcaaaaa tcaagaagtt
    23041 cgtgcacaag cattggcaac tttcaaacag caaattgctc tgtgctctgc ctatcaagcc
    23101 aagatgattg ctacggaaac cggtagtgtc tctgtcggtt acacaaaaga aaattttact
    23161 gaagaagctt atcagatcgc cagaaattct atcattgaat tagtcgctca tgcagaaaat
    23221 tttggcgtaa ctgttgctgt tgaagcaggg atcaatcatc caatctataa ctatcagtta
    23281 gctaaacgtt taatcgatga agtagcttca ccaaatttaa aaatcatttt agattgtgcg
    23341 aatttgatgc ataaagaaaa tcatgctgat caggaaaaag tgatccgagg tgccttggac
    23401 aatttacatg gttatattac agcgcttcat ctcaaagact atatcatgga aaatggaaag
    23461 atccgcatgg tacctgttgg caagggctgt ttagatttcc gcccggtact gcattatatc
    23521 aaatatgagc gtcctctaat gtacgccact cttgaagcta ctcctgagat ttatgttcct
    23581 gaagcaatag aacacttgca gtttatttac caagattgtt aaggagaaga ttatggagga
    23641 acgaatacag aaaagttatc aaaaatatca tgcaaatgct tttcagacat tgctgcatct
    23701 ttatcaggga aattattggc gttttgccat gtcaggactc ttttttatta tcaaacatct
    23761 tccatcttgg ttaatgccga tcgctattgc caatgtaatc aatgcagctt catcgaaaga
    23821 ttcttctcag ttgcaggcca tttatttcaa cgcttttttg atggcttgtc tgattggtca
    23881 aaatatattg acgaattatt ttcatgtaaa ataccatagc acttcgatcc gtcaagtaga
    23941 agctggacta agagcgaccc ttatcaagaa aatccaagaa ctatctattc cttatcaaaa
    24001 agaattgcaa tctggacgaa tccagtcaaa gatcatccgt gatgtagaag ctatccaaac
    24061 gttgtcctct caagtatttg tcagcggatt aaatatcgct gtcaatttat tcgtagcttt
    24121 aggtatcacg ctttataaaa gtcctgttgt tttcatgttt ttcttattga ctgttcctct
    24181 cgccgcattc gttactctct ttttccgtaa aaggatcaga cagtccaact cagacttcag
    24241 gcaagaaatg gaagagactt ctgcaaaagt gatcgaagtg atcgaattag caccgattgc
    24301 tcgagctcat gggttagaaa aacacgaaac aactcgctta aataagcatt ttactcaaat
    24361 ctatgaaaaa ggattccact tagatatcct gcaatctgtt ttcggatcag tcagctgggc
    24421 tagttttcag atatttcagc tgatttgttt gattttcact gggatattag ctttacaagg
    24481 gcgcatccaa cccggcgatg tcgtgatgta tcagacgtat ttcacgacga tcgtcaattc
    24541 cgtttctgga atgatcacgc tgattcctac gatttcaaaa ggcatggaat ctatcacttc
    24601 tattggcgaa ctgttgattg ctggcgatgt agaaacttat cacggtaaga aaaaaatcca
    24661 acatttggat ggaaattttc gttttgagga tgtttctttt ctttatccgg actcactcaa
    24721 accgattatc gatcattttt ctctagaggt gaaagctgga gaaacaattg cttttgttgg
    24781 gccatctggc tctggaaaat ctacactcct taacttattg attggattta tccagccgac
    24841 ttcaggaaaa atttttctag atgatcagcc tttggatgaa ctggatatgc gaagtgtgcg
    24901 aaattattta gccgttgttc ctcaaaccac tttgttgttc tccgcttcga tcaaagagaa
    24961 catcacttat ggcttgaaaa acgtatcaaa agaacgttta gatgaagtca tcgaagctgc
    25021 gcaactttct tcactgattg accagcttcc tgatggtctg gataccttag taggcgaaca
    25081 tggcaacaag ctgtctggtg gccaaaagca acgtatctcc attgcacgag cgttgatacg
    25141 tcagccaaaa atcattttgc tagatgaagc aacctcagct ttagataacc agtcagagaa
    25201 aaaaatccag caagccctga attacctgac agaaacaccc actactttta ttgtggctca
    25261 tcggctttca acgatcaaag aggcagataa aatcgttgtg atcgatgaag gaaaaatcgt
    25321 tgaaataggt acttacgaaa aactgttagc tcaaaaaggc tatttctacc gcttgaaaaa
    25381 tgaatgacaa aaaataggag ctatgacaag acttttgtcg tagctcctat ttctgaataa
    25441 acggtgttct cctgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc
    25501 tgttttttga aattgttcct tgtaactcag agttaaacgg taggctcacg acctcttggt
    25561 aaatggtgtt caagagacag cctatcggca ataagaagca attttgtcat cgctcaaggc
    25621 cgaacgtctc tttcacaccc tctgttgttt cataaaatag ttttattcgc tgtaaaggtc
    25681 gcgaactggt gtcatgatga tacggttcaa gtcattgatg attgtactga agccttgctc
    25741 tttttgcatc aagtcattga ttacttcttc tgattggatt ttcacagcca tttcttgtgc
    25801 tttcattgca tcttcgtcgc tgaactcttg tccactcatt tgtttttctt gtaattcgat
    25861 ttgcatcgct tggaagtctt tgaacagttt gtgtgcttct tcgtttgctt gtactttctc
    25921 gtacgcttct ttcaacgctt tgaattctgg taattcacgg atctcacgtt cgatttgatt
    25981 cgcgctatcg tagatgtttg acatgttatg ccctcttttc ttttagtagt tactctctat
    26041 tgtaacatgg ggttattcgt tttcaaaggg gctactctcc ttgatatttc cgccagaggt
    26101 ctttagccgc ttcaccaatt ccctgaacgc cttctttcac catatcgccg aagtttttca
    26161 gcccttcttt tgcttgttca ccatattgat tcaagttatc ttgccaattt ccagactctc
    26221 cattagaatt tcctgtgttt ccaacttgat ctgcagctac cacttgtcct cctgtggcat
    26281 aggcatctgc tacactaaat cctgtttttg aagtatacgg aaggatgcct tctgccgctg
    26341 atttgaagac tttcccgaca acatttccac ttgtcccttc caaatagtgc agcttgctgg
    26401 attcttcgta acccatccat gtcgaaatca ctacgtctgg cgtgtaaccg atcatccatt
    26461 ggtcatttgc ttttgtggca tcgaaattag tttctgtggt tcctgttttc cctgctatcg
    26521 tataacctgc aggattcgca gcagccgctg ttccatttga aaatgtacca agaagcatgc
    26581 tggtcatttc atcggctgtt tcctttgata tgacctgttt tggttttttg tttgtgttat
    26641 cgacgatcac cgctccagtc gaatcaacga ttttcgtgat aagatgcggc gtatacatct
    26701 gaccatcatt ggcaaatgca ctgtaagcac ccgccatgat caatggagat actccttttt
    26761 ccagtccacc aagggctaag ccataatact tatcagattt tgctaaaggc aggccgaatt
    26821 cttctgcttt atcatatcct ttttgtaaac cgatctcatg caaaagccat acagtaggca
    26881 aattcaaact ttgtgcaaca gcttgataca tcggtacttc tccttggtat gtcccgtcaa
    26941 aattatgcgc atcgtaataa ctttgcggtt cgtctttcaa gatactggaa ggcttgtatc
    27001 cagcttccag tgctggtgta tatacgctta atggtttgat cgttgatcct ggtgaacgac
    27061 gcatctgtgt agcaaagctg aatcctctaa agacatgatc tcctcttcgt cctactagtg
    27121 cttgtacacc acctgtattc ggatctaaag ctactgaagc agattggacc atggcaccat
    27181 ctgaagcatt tgctgggaat aatgcactgt tttgataagt agcttctaat tgcttttgat
    27241 aatcctgatc taaagctgta tagattttat agcctttatt catgacatct tcttcatcaa
    27301 gaccatactc gttaactgct tcatcgatca ctgcatcaaa ataataagga taacgataac
    27361 cagaatctga attatcgtat gtgtcattaa gatacgcgct gatatctgtc tggctttctg
    27421 cttgttcttg ttcttttgtg atttttccgt tttcttccat tacacttagt actgtgtttt
    27481 tccgatcatt cgcatttttt gggtgatcga tcggattata aataccagga cctttcagca
    27541 ttcctgccaa agtagcagcc tcagacagcg taacattcgt tgcatctact ccaaaatact
    27601 ttctggaagc gtcttggaca ccccataccc cgttgccaaa ataggcattg ttaagataca
    27661 tggtcaagat ttcttctttg ctgtacttct tttcgatctc gattgccaaa aataattcct
    27721 tcgcttttcg atcaaacgtt tggtctaatg tcagatacgc atttttggca agctgctgag
    27781 tgatcgtact tcctccgccc cctgatgttc cgccagacaa gaccattcgg actgctgcac
    27841 gggcaatccc tttgatatca aacccgtgat gctcgtaaaa attcctatct tctgtcgaga
    27901 ttactgcatc ttggatatag ggagaaatcg cgtttaattc tacatatgtt cctttttggg
    27961 cgtacagtgt gccagcttct tcgtttttct tatcataaat gactgtcgat tggcttaatc
    28021 ctgattttaa cgtttcgaca tttgcttgtt ttgctaaaat aaagaggtag atacttgtca
    28081 ctaagacaca gatcaaacca atcagcaaaa tcaatttgtt cacatgatat tttttccaga
    28141 tgcgctttcg ccatcgatgg aattgtccga gatatggtct gatccaacgc caaaaactga
    28201 tcaaagcagc ttttatcttt tctaaaatgc tttgaaaatc catttgcttc ttctcctgtc
    28261 tatttgattc aatcgtctta ttttaccata gtgaaagagt gttttcttcc tgcttaagtt
    28321 gtagttcaat atgttttttt acgttagtgc aagtagagta acataagaat gaacagaaaa
    28381 atgtaagttt tttcttaaat atgttttaaa aatggtacaa ttagtctgcg attgcactga
    28441 aaggagtaca cttgtgaaaa tcacgatcac tttacctatg aaccatcctg aaacaacaat
    28501 tcgcgacttg cttgaaaagg aatggttagt tcctagaaag gttcgacact ttttgcgtat
    28561 acgtaaaaac gtttggatca atcaacaacc tgctttgttc catcatgaag taaagggcaa
    28621 tgaccagatc acattgttat tcgaagaaac agattataat tatcaagaag ttcaattagg
    28681 agatgcgaag cacatccgtg ttttatatga agatgagcat ttgattatag tggataagcc
    28741 tgcaggaatg aagactcatc caaatgaacc gacagaaaac agtaccttac tcaatcattt
    28801 agctgcttat ctcgacacta aacagcaacg accttacgtt gtccaccgct tggacaaaga
    28861 aacaagcggt gccgttttat ttgcaaaaaa tccatttgtc ttgccgatac ttggaagaat
    28921 gttggaacaa aaaatgatct atcggagata tcaagccatc gtatggggag aaattgccaa
    28981 agatatgacc atcaccaata aaattggacg agatcgccat gatcggagaa aaagggtcgt
    29041 agatgataaa aatggtcaga gcgccttgac acatgtcact gtcaacaaag tactttctga
    29101 acaaacacaa gtctattgtg ttttggaaac cggacgtaca caccagatca gagttcattt
    29161 agctcataag ggacatccga tcgttggaga ccctctctac caaaatagaa cagctgaacg
    29221 actgatgctc catgcattag aactgcacat gatccatcca tttactcaag aaaaaatagt
    29281 cggtaaagct ttacctggat tatgggaata gaaataaaaa agaacagagt cttatcagca
    29341 gataatgata agactctgtt ctttttgtgt aatttgtctg cttacttttg atgttcgtgt
    29401 tctgctaaca gcaaactacc gataatccca ctttcattcg gcagtcccca acgaacgata
    29461 tattcactta cttgaggtgt ttccatataa ccacccatca attcaacaaa ttgctgacgg
    29521 attttttgaa ggagatgatc ctgattcatt accccaccgc ctaaaataat cttttcaggt
    29581 gctaacgtca acgtatagtt gatcagtgct tgagcaatat aataagcctg aatagcccaa
    29641 acaggatgat cttgcggcag atcttgccct ttgatccctg tgcgtgcctc aatagacggt
    29701 cctgctgcta atccttctaa acaatcttta tgataaggac aagtaccttc ataatggtca
    29761 tctggatggc gtttcaccca aatatgtccc atttcaggat gtgcgatccc tgaaaaaatt
    29821 tcttcattga tcacgacacc tgcaccgatt cctgttccca cggtaaggta aatacaacta
    29881 ttttttcctt gtgctgctcc ttgcttcaat tccccatatg cagcagcatt gacatccgtt
    29941 gtccaagcag caggaatatt gtagcgttcc ttgattgtgc caagaaaatc gaattgtgcc
    30001 cacccttttt tgggagttgc caaaacgtat ccgtaattat cttttgtttt gtcgatacca
    30061 atcgggccga atgagccaat cccgattccc ttcaattcat aacgatcaaa aaattcaaat
    30121 acctgatcca atgtttcttc tggtgttgtg gtagggatac ttaatttttt cttaattgtc
    30181 aaattaagtt agacagatca tcaatactta cgtaactcaa aaatacttcc aaaggtgttc
    30241 gataatccag tgattttcta ggaatattat ttcgtttaga tgcgacagat tgaataaaaa
    30301 attcatctac tgaattgaaa tccatagatt tcaataaacc atctctacgt aacaatccgt
    30361 tagaattctc gtttaggcct ctttgagacg gcgttcctgg atcagcgaaa taaatggcaa
    30421 tatcattgac attactgatc tgtttccaat ttgaaaattc ctttccacaa tcaaaagtga
    30481 tggatttgaa taggtttttc ggtacagatt caaaccattg atttaatttt ttttcaatat
    30541 caatcgcttg tctaccacac ggtttcaatg tgatgataac ttttgataat cgttcaacta
    30601 aggtaattac agcactttta tgtttcagac ctacgatagt gtcaccttca aggtgaccaa
    30661 actcatttga gaattggcta taatcctttt cacgttcatg aatagagcgg cggaaagttt
    30721 gttttccacg tctttcttga tgtccattcg gtttacgctt gcctttcatc ggtaagtcag
    30781 aagaatcaaa tagccccttt ttgaacatac gataaatggt acgagcagaa caactaatag
    30841 gaaacgctgc acgaccaaca atcacatcgg gtgtccatcc ttgaacaacc tttctttgaa
    30901 tatattctga ttgttcctcg ggtaaaacaa gcggacgtct accacagttt gatttgtttt
    30961 tcttatactg ttgataatat tctaaggctg attttccttg cttgaagaac agatatactt
    31021 tatggatcgt ttgtcttgaa cgattcaagc aatgggc
//
LOCUS       DAZKMK010000022        30140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000022 DAZKMK010000000
VERSION     DAZKMK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.022"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..176)
                     /locus_tag="ACG8V1_001047"
     CDS             complement(<1..176)
                     /locus_tag="ACG8V1_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0329155.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAK"
     gene            complement(311..991)
                     /gene="cmk"
                     /locus_tag="ACG8V1_001048"
     CDS             complement(311..991)
                     /gene="cmk"
                     /locus_tag="ACG8V1_001048"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0329156.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(1040..1675)
                     /locus_tag="ACG8V1_001049"
     CDS             complement(1040..1675)
                     /locus_tag="ACG8V1_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0329157.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(1741..3144)
                     /locus_tag="ACG8V1_001050"
     CDS             complement(1741..3144)
                     /locus_tag="ACG8V1_001050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0329158.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(3131..4162)
                     /locus_tag="ACG8V1_001051"
     CDS             complement(3131..4162)
                     /locus_tag="ACG8V1_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329159.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            4193..4414
                     /locus_tag="ACG8V1_001052"
     CDS             4193..4414
                     /locus_tag="ACG8V1_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0329160.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(4579..5316)
                     /locus_tag="ACG8V1_001053"
     CDS             complement(4579..5316)
                     /locus_tag="ACG8V1_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0329161.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(5469..6809)
                     /locus_tag="ACG8V1_001054"
     CDS             complement(5469..6809)
                     /locus_tag="ACG8V1_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0329162.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(7046..8569)
                     /locus_tag="ACG8V1_001055"
     CDS             complement(7046..8569)
                     /locus_tag="ACG8V1_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0329163.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(8947..9558)
                     /locus_tag="ACG8V1_001056"
     CDS             complement(8947..9558)
                     /locus_tag="ACG8V1_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0329164.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(9681..9806)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(9888..10604)
                     /locus_tag="ACG8V1_001057"
     CDS             complement(9888..10604)
                     /locus_tag="ACG8V1_001057"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0329165.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(10610..11179)
                     /gene="scpB"
                     /locus_tag="ACG8V1_001058"
     CDS             complement(10610..11179)
                     /gene="scpB"
                     /locus_tag="ACG8V1_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0329166.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(11163..11933)
                     /locus_tag="ACG8V1_001059"
     CDS             complement(11163..11933)
                     /locus_tag="ACG8V1_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0329167.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(11930..12301)
                     /locus_tag="ACG8V1_001060"
     CDS             complement(11930..12301)
                     /locus_tag="ACG8V1_001060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0329168.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(12320..13207)
                     /gene="xerD"
                     /locus_tag="ACG8V1_001061"
     CDS             complement(12320..13207)
                     /gene="xerD"
                     /locus_tag="ACG8V1_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0329169.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(13303..13776)
                     /locus_tag="ACG8V1_001062"
     CDS             complement(13303..13776)
                     /locus_tag="ACG8V1_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0329170.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(13881..14744)
                     /locus_tag="ACG8V1_001063"
     CDS             complement(13881..14744)
                     /locus_tag="ACG8V1_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0329171.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(14940..17039)
                     /locus_tag="ACG8V1_001064"
     CDS             complement(14940..17039)
                     /locus_tag="ACG8V1_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0329172.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(17259..18368)
                     /gene="rpoD"
                     /locus_tag="ACG8V1_001065"
     CDS             complement(17259..18368)
                     /gene="rpoD"
                     /locus_tag="ACG8V1_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0329173.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(18392..20233)
                     /gene="dnaG"
                     /locus_tag="ACG8V1_001066"
     CDS             complement(18392..20233)
                     /gene="dnaG"
                     /locus_tag="ACG8V1_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0329174.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(20415..22730)
                     /locus_tag="ACG8V1_001067"
     CDS             complement(20415..22730)
                     /locus_tag="ACG8V1_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0329175.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(22915..23775)
                     /locus_tag="ACG8V1_001068"
     CDS             complement(22915..23775)
                     /locus_tag="ACG8V1_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0329176.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(23834..24466)
                     /locus_tag="ACG8V1_001069"
                     /pseudo
     CDS             complement(23834..24466)
                     /locus_tag="ACG8V1_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            24633..25394
                     /locus_tag="ACG8V1_001070"
     CDS             24633..25394
                     /locus_tag="ACG8V1_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0329177.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            25420..28011
                     /gene="mprF"
                     /locus_tag="ACG8V1_001071"
     CDS             25420..28011
                     /gene="mprF"
                     /locus_tag="ACG8V1_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0329178.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(28199..29386)
                     /gene="tuf"
                     /locus_tag="ACG8V1_001072"
     CDS             complement(28199..29386)
                     /gene="tuf"
                     /locus_tag="ACG8V1_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0329179.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            29754..30110
                     /locus_tag="ACG8V1_001073"
     CDS             29754..30110
                     /locus_tag="ACG8V1_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0329180.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         8359 a         6361 c         5213 g        10207 t
ORIGIN      
        1 tcttttgccg gaacgactcc ttcaactcca gccccttcga ttccgacaat cacttgtttg
       61 tcttcaatga ctaaaacttc gcccttaaca acatcgccaa cttttacttc ttgtacgctt
      121 ttcattgcgt cttccattga ctggttgttc tcttgatttt gatcaaattc tgtcataaca
      181 cttgtcctcc taaccaacat tctgccgtaa gtacggtact gtttttagtt taattgatta
      241 ttcaaaaaaa ataaagcgat ttcgttcgtt ttccttgaaa aaacaattgt ttttcatttt
      301 ttttcttaat ttaaaaaaga agataacctt ttttcaaaac aacagctttt attgctgcaa
      361 cgacttctgg aatcgatttg ccagtcgtat cgatacgaac agcatcctct gcttgcttca
      421 gcggagagac gtcccttgtt gaatcataat ggtctctttc ctgtatctct ttcgtcagtg
      481 tttcaagatc agtcggaatt cctttttctt ggttctcttt gaaacgtctt tctgcacgct
      541 cacttacgct tgctactaaa aagattttca cttctgcatt cggtaataca gctgtcccga
      601 tgtctcttcc gtccatgaca acgccaccgc gttcccctat ttttcgttgg acagcaacta
      661 attcttctct gacttttcca tgtgcagaga cttcagaaac agcttttgtg acatctggtt
      721 gacggatttc taaagtcaca tcttttgatg ctatgaatac atgctgtcca tcttcttgtt
      781 gcgaaaaaga gataggatac tttttgatca gcgaaactag cccttcttca tctgaaaaag
      841 atacattgtt cgtaatagct aaataagtta ctgcgcgata catggcaccg gtatctgtat
      901 aaatataatt caattctttt gctaatatct tggcaacggt acttttccca gatgaagcag
      961 gcccgtcgat tgcaatactg atttgtgtca tgatataact cctttttctc agaaaaaaag
     1021 ccgcaaagcg cggctattat tatttaattc ttaattcttg accagggtaa agcataaagt
     1081 tgtttggatc taagccattc aattcaaaca attgatcagt ggtgatccct gcacgctcag
     1141 ccacttgttt tggaccttcg cctgcttgaa cagtcgttgt tgaaccggct gcagctgatg
     1201 aagaaggtgt tgtttctgct gcattgctgc ttgaatcccc tgcttgataa gttgcctcac
     1261 ttgaggaagt cggtgttgtc tctgctgcaa tactgctttc tgacgttaca cctgtatctt
     1321 gagacacagt agattcttcc gcgattgtac tagattctac tgttgaagtc tcagttgact
     1381 cctcttttgt tgtacttgta gaactttcta ctactgatgt cgaagtttca gaagaagttg
     1441 ctgctgttgc attgttgctt gatccatttc tgatccagaa ataagcacct gctggaatag
     1501 cgatacataa gaccagtaaa atcaaaaata gcgaaagaaa taatgtattt cctttctttt
     1561 gttgtctctg agaacttctt gatgaaactt catcatctgt atcgtagatt ggttgttccc
     1621 atggttcttg tgtttcgtta ttttctgtag attgatgtct atcttttctg ctcactgttt
     1681 tacctcctaa actctatcgt gtgtattata ccataggaaa aagtgtcttg tcctaaaaaa
     1741 tcaatgttcc atgttttttt taaataaatt tagtaaaatc gttccccaat gaagttgttc
     1801 agtcttagaa aaaagcttcc tctttggcag aagttctgct tttgctccat caatatcaca
     1861 acaattctgc ggcttttcgg agagtttttc gccaaaataa gctaagatga attttctgcg
     1921 gcagttttct tcattaatat agcgaagcat ctgctgcagg cggaattctt tttctttttc
     1981 ctgacgtttg agccgttcca cccaattttc tggtttttca gactgctgga tcagctccat
     2041 ccattttttt tgcagctcat caaagggagc ttgctctgtg gcatattcca aataaagctc
     2101 aaaactttgt cgctgttctc tagaaagttg attaaagaaa taatggatac gttcatcccc
     2161 actttgataa aaaagaattg ctgcactttc ttcctgatct ctgcctgctc gaccgatttc
     2221 ttgtacatag ttttctagac tgtctggcaa atcataatgg accacataac ggatatcaga
     2281 tttatcgatc cccataccaa atgcattcgt agcaatgaga aactgcagct gattttttac
     2341 aaactgctgt tgaagttgtc ttctttgagc tgcatcaaga ccaccgtgat agtatccaac
     2401 tgtaaaacgt tccctgaata aatgatacaa tcgttcgact tctttttttg ttgcacaata
     2461 aatgattgcc gctccttgtg ctctttccat gaagtgttca agttcttgtt ctttttgctg
     2521 cgttttccga acaaaaagcg agatattttg ccgattgact gattgtctga tctcttttgg
     2581 tggtcgatca aatagtacat gacgtatatc tcttgctact aaatctgttg ctgtcgccgt
     2641 tagtgccagt gtgaccgggt ttcctaaaag ctttcggatt tttcctaact gttgatattc
     2701 tggacggaaa tcaactcccc attgagacac gcaatgagcc tcatctacta catataacgc
     2761 aatattttgt tttttcagat gctctaaaag agaaggatta gtcaacattt cgggactcaa
     2821 aaacaaaaat ttatattgcg atagatgacg gaggacgtat cttctttcag attgcgaaag
     2881 taaactattg aaagcagcta ctcgtttctc ccctcgtttt tgtaattgcg tcacttgatc
     2941 ttccattaga gaaagcagcg gtgacacgat gatgaccaga ccttccatta aataccccgt
     3001 caattgatag caaaggcttt ttcccgttcc tgtaggcaaa atagcgagag tatcttctcc
     3061 atcaagcaac gcctgaataa tttcttcttg gccgggacga aatgaatcaa atccaaaata
     3121 tttttttaat tcatttatta acattctgta tcgcttcctt tctcaataga tagatttgat
     3181 acaaacgaaa ttctccataa tctaattgtc catctacatt ccattcgcta aagcgccact
     3241 ctcgcacgga atccttattt ggttccaaac gttcgattgt ttctgcagag agaatccact
     3301 cgtatggaaa atcatcaaaa aacaaggccc actcaataag atgatctgtt actgttcctc
     3361 tttttaaatg acggattgcc atgatctggt caattgtctg accattcata aacatctgct
     3421 tggtgatcat catgctttga ttgaaatttt gctgtaataa tgaatcgatc aaagcgtgcc
     3481 aaagcgaatc tggccgtttt tcgatttgtg ctaacaaaag gtggatacat ctgctttgtt
     3541 gcagataaac cgctgactca tcattgtttt ttgccagctg ataaggaagc agccctgttc
     3601 gctcatttcc agataattgg ttagctaaaa aatctgctgc ttcttctggt atctccgaaa
     3661 acagctgcac caaactctca tacgcagtat caatcaaaat ttcacgtggc aattggcttt
     3721 cactaagcca tttttttagt tggaaagtat aaaaaggact tgtctctgct ggaagatagt
     3781 cttgaatacc cgttgccaga ttagaaatta cttgaacagc aaatttaatg agccgccaac
     3841 tagtttcccc tgttctgcca tagcggtcaa aacgcaaacc agtatgttct atttgatgtt
     3901 cggacaacat atcagcccct gcaggcgtta gctttgcttc attttcattt atttcgatcc
     3961 aaccttgctg cgcaattttt tgcaaaattt gataaaactt atcctgcttc aattctggaa
     4021 aactgttatg gataaacaaa agttcgtggg taaatccata aataagtact gaagtcgttc
     4081 gttttcccac aaggagttgg taaagagaac taatccgcag cttgtgtcct gatgaaaata
     4141 gcgataaaat aaaaacaatc atattattct ccttaagtta ggatggttag aaatgtcact
     4201 actatgtaaa cttgtacctg aacgctgtat tgcatgcggt ctttgtcaaa tcgaagcgcc
     4261 agatattttc gattatgatg aagaaggaat cgttttattt gcccaagaac cagaggctca
     4321 tcaacagttc gtgatgccgg aagaagaaac atatgtaaaa aaagcgtatc aaaaatgtcc
     4381 agttcgagcg attttattag aaaataaaat ataaatataa acgaaacttt aaaaacgtac
     4441 actaagttct ctaaacttga aaataagccg ggatctgttt ctcttgttcc gtgaaatccc
     4501 gacttatttt gaaagattca tttgctatct atagttaatt gactagtttt ttcacggacc
     4561 ctataggacc catttatatt atttcaactt tttcagccag ttttccaatg tattgacgat
     4621 cacagtttgc tggtctgcgt tagaaatttc tgcctctcca tctccttttt gctcaccata
     4681 gctgccaaaa cctccatgat ttccgccatt gattgataca tactctgttc tttccggtaa
     4741 atactgctta gcttcttgat aagcttcctg attcaatact ttatcttttg tcgccgtgat
     4801 cgataaaaca ggaacagctg attctgtcaa tgttcctttt ttgtcgggat aacttgccat
     4861 gaagaatacc ccttttaaat gtttgtctga atgattctta ttgacataac gacttgccat
     4921 tacgccacct aatgaatggc caccgatcac ataatcggaa tttggaaaat cttctttgac
     4981 cttatccgct ttgtttccgc ccatcactgc tagatcaagt ggcattttga caatagcgac
     5041 tgaaaagccg ttttttgcta aatcttgcgc ccaaatacta taactttctg gctctacgag
     5101 agcaccagga taaaaaataa gtaatggaga ctttccatga ttctttttct ctccttcaaa
     5161 aaataagtaa tcttctttgt tttttccttc ttttaccaac acttgcgctt ctgacgatgg
     5221 ttcgtacgta ttttgctgta tatagtaata accggctcca ccgagtaatc ctaaaatgat
     5281 gactaagcca attagtattc gtctaaatat tttcactttc tcccaccttt tctatctgag
     5341 tgaccccttt ttcttaatca agtatgatta taccttaaaa aaataagaga gaaatgcaaa
     5401 ctttccagca tcaaataaaa aaagacggaa tatccatttg aatatccgcc ctttttgtat
     5461 gtttattttt atttagcaca taaacgatac acagcttctg cataaatttc cattgcttta
     5521 tacatactac ttaattccca tcgttcattc gcctggtgca taaagtcagg agtatcaggg
     5581 aacatcgcac cgaatgccac acactggttc atcgtacgag caaaagtagc gccgcctgat
     5641 acaaacggtt cagtcatgtc tccagtaata tcgcgatatg tccccaatag atttttcact
     5701 aactcactgt ctagtggcac gtataatgaa tcaaggaagt caaattcttc ataagtcaac
     5761 ccatattttt ctgctgtttt agttaattta gctactaaat catctttctt gaatgtaaca
     5821 gggattctca tatctacacc gatttttgtt tgttctggtg tgatttccaa acttgcaaag
     5881 ttcatcgtta gctcgccgga ttgttcatct tctgttttgc ccacgacagc ttcaccagtt
     5941 gcattttctt gaactaattt tcctaagaaa ttgattgggc caaaatcaaa tacttctgaa
     6001 agagcaattg ccagacgaga aatagcattc actccttgtg ctgcatcttt cgcgtggata
     6061 cttttaccaa gaacgacgat gccttcacct tgttcttcga aatcgaattc gtgttttttc
     6121 aatgcttctt ttacttcaga aagcttttct ccagagtaag gagctgcatc aggaacaaca
     6181 ttcaacgctc ctccagcttt tacagaaaac tcgttcgttc ctggtcctgt caagtaagct
     6241 tgcaataagc ctttttctgc ataaattaaa ggaaattcag catctggcgc aaatccttgc
     6301 gtaattcctt cttctttttc gttatatttt tctaaacaac gccataaatt ttcttcatct
     6361 gtaccaaaga taaaacgaat acgtgtcttg aattccacgc cagcatcaat caaagctttg
     6421 acggcataca ttgccgcaat agaaggacct ttgtcatctt gggatccgcg accaactaac
     6481 cagccgtctt ttatagttgg atcgaatggt tttgtttccc aattattttc gtcccctgct
     6541 ggaacaacgt ccatatgaca caagataccg aataattcat ctccagagcc aatctctgaa
     6601 taaccgtaat agccttctgg atctttaaat gttctaaatc ctagcttttc actgatttcc
     6661 aatactttat ccaaagcttc aatcactttt ttaccgaagg gatgtccttg tcctgaatct
     6721 gcttcatcta gtacggaagg aattcggatc aattcgctca atgattctaa tgcttgttca
     6781 tgatgttgtt ctgtcacaaa ttttttcatg atattatcct cctaatatgt taaaaagggt
     6841 catgtcaaat tcctccgaca caacctcttc ttctcaaata atgacgtttt aagcctgctc
     6901 catcagcaag cagaatgttc ttaacaaatg atgaaagaaa aaacactcac tgaatggagc
     6961 tattgactta tccacgtgct tatcgatagc cgtttatcgg tttcttcatt cttatgatag
     7021 accttgtacc tgtatttgtc taggtttaga acattgctgc tacacctagg atcacacaga
     7081 tggctacaaa taatatcgca attaatttac cggtaaattt ccaccacact gaaacatcga
     7141 aacgagcaat tgctacagca cccattacga tagcagatgt tggtgtaatt aagtttacta
     7201 aaccagatgc agattgataa gcagtgatta ctaaatcctt accgacgccg gcaaattcac
     7261 ccataggtcc catgattccc attgttgcag cagctaagcc ggaagttgaa ggaatcaaga
     7321 atgacatagg gatatagaaa ataaatgtaa taatgatgaa tacacttgaa gataatgaac
     7381 ttaatccttg ttcgccccaa tgtaagatgg tgtctgtgat taatccattg ttcataacca
     7441 cttggatacc acgagcaaca gctactacga ttgctacacc gatcaaatca gaagcaccag
     7501 ctaaaaattc agagacaaat tcagactctt tcatgccata agtgattgct actacgatgg
     7561 acattactaa gaataacata gtaatttctg ggaagtacca gtcacctaat ggaagcatgc
     7621 ttttacctag aaccgtacct agtaccggaa ggtttgttaa ccagtttgtc aatgattcaa
     7681 aaatcgtgaa attcgcattc aaggttgtcc aaggaatcaa actgatgatc atgataccaa
     7741 aagtaataaa gaacaataca ttaacatgtt tttgtttttt cgtgattact tcttgacgac
     7801 cgattgcttc aatatcgaaa tgcttcatat cttctttacg ttggttgtat actaatgatt
     7861 ttgttggatc tttttcgatc tttgatgcat aacgatacac atatacaata gaaatagcta
     7921 acaaaataac gaacatcaag aatcgaagac caataccttc acccatcgaa atcccaacag
     7981 cttgtgaagc tacgcctgtt gcaaaaggat ttaccgttga agctagacag cctacttgag
     8041 accctactaa tacgattgct acagctacta ctgtatcaaa gcctacagcc atcatgactg
     8101 ggatcagtaa agcatagaat ggaatcgttt cttctgccat tccgtaagta gagccgccta
     8161 atgcaaataa cagcatcaat actggaataa gcattttttc ttttcctttg aatttcttca
     8221 caactgaagc aatcccggca tctagtgccc ctgttttgtt gataatacct aaaaatccac
     8281 caacaaccaa aatgaagaaa gagattggga ttgcagcagg tgtttcttta gttagctccc
     8341 ccgctggtac acctagcatc ccattgattg gcgccatgaa aatatcccac agcccttgag
     8401 gattttgttg tactcgttca tatgatccag cgacgatgtt gcctgcatcg tctacactat
     8461 agtgcccagc tggtataaac caagtaagta cagcgattac tgcaataata ataaataaga
     8521 ccgtatacgc tgaaggcatt tgaaattttt tcttttttgt ttgttccatc ttcttcttcc
     8581 tttcttttcc gttagagaca acaattttgg ctctaatgtt attttatcaa aaagagtatc
     8641 agaacaaaaa taataacacg cattttgtaa aacttttgtt caaaaagttc gtttttccct
     8701 attccctcct cttttttcag gtttttaaag ctttcttctt tttcattgta gcgtttcctt
     8761 ttttttataa acaaacattt tcgtctttta aaaggagaag aatttcacat ttgcaaagaa
     8821 ttgattgatt tttatgcata aatcaaaaaa atactcaaaa caaacaaata aaacgtttac
     8881 attattgcat gtcttgtatt tttttccaaa aaaaaagacc aagaaatttc ttgatctttt
     8941 tgatatttat ttgactgctg tatgacgttg ttcgaactga tgttgttttc gacttaacca
     9001 tggaagaagt tgcgcatgca gcactaaaaa gacagcactc acgatgaatc ctttgatcaa
     9061 gttgaaagga acgatcccaa tcagtacata gtttgctaaa gacatgccca gcatctgtgt
     9121 tgcgttcagc cctaaaaaca ttaagtaaag aggcgtaata acgaaataat ttgcgatact
     9181 cataaaaata gttagagcga tggttcctgt aatgacacct aaaaatttgt ttcttccttt
     9241 atgctgtttc tttacactaa agaaataata aatcgggaat gtaaagatcg ttgttgataa
     9301 gaaactagca gtgtctccta ccaaattatc cggagaaaaa cctgtagtca gcagatgtaa
     9361 tactgaacgc aagaacgctg ttgcaactcc ggccgctgga ccgaataaga acatgctgat
     9421 caataccgag atatcactaa aatcgatctt caaaaaacta aaagccggca tgatcggaaa
     9481 agcaaaatac tgtaaaacta atgcgatcgc agcaaacatc gctactgcaa ccattttctg
     9541 tacacgggtg ttcttcattt gaaatcctcc tgttaggact cctcttcagc gaactttttg
     9601 acgtttctct ccaaatgaaa aaactctatt ttccagggaa aatagagtta gagttatttg
     9661 atcaagcgtc gtcaaataag ctctatcttc tttatccaga ctatactgtc ggtatcggaa
     9721 tttcaccgat tcagctattt ttttgcaaaa taggtcgtgg actataacca ccggtcggga
     9781 atttcaccct gcccctgaag acgaacctta tataattttt gtttacatat tcattataca
     9841 tatctattca aaaaacgcaa catctaacac ttataaaaag taagtgttca ttttgagtgg
     9901 ttcagcaaac tgctgacttc ttttttgttc agttcacggt aatccccagg acgtaggcct
     9961 tgtaaagtca gatctccgta tttttcacgt tttagttttt gcacaggaag accaactgct
    10021 tgaagcatat ttttcacttg atgattacgt ccttcgtgga tcgtcaattc cacgatactg
    10081 tggtttgtct taggatccac tgataaaatt tggaatttag ctggagatgt ttttcgtcct
    10141 tcgatcttta ttccttttgt caaaggtgca agcatcgatt ttgtagcgat cccttttacc
    10201 tttgcaacgt aaactttatc cacttcatgt tttggatgtg tcaggcgctg agcaaaatct
    10261 ccatcatttg tcagtagcaa gatccccgaa gtatcgtaat ccagtcgacc tacaggatag
    10321 atacgttctt ttaccatcgg caaaagatcc gtaacaactt ttcttccttt gtcatctgca
    10381 acagcagaaa taactccttt aggtttgtac aataaatagt aaccgtattc ttcttgataa
    10441 attgggactt catccacttc cacagtgtca tgtttcccaa cttttatccc taattccctt
    10501 acaacttgtc cgtttacttt tacacgtcct gcaaggatga attcttctgc ttttcgacga
    10561 gaagtgatgc cagcgtgggc aattactttc tgcaatctct ccattacaat tattctcctt
    10621 tattcattcc atcaaagaat agatctggtg acacgtcttc ttcaagtgaa atttccattt
    10681 cttgtgtatc aggcagttct tctaatgatt tcaaaccaaa ataatctaag aagtagccag
    10741 ttgttccata taatatcggc cgacctggtc cttctactct acctttttct tcaatcagtt
    10801 tatgggcagc tagacgctgt accgctcctg aaacctgaac tcctcgaatc tgctcgattt
    10861 cagctcttgt gattggctgt ttataagcga tgattgctaa ggtttcgacg gcggcttgag
    10921 ataatgtgtt tgatgtccct tgcgcatatc gcttaagtaa gggagccagc tcttttttag
    10981 tcgttaatac ataatggctt tccgtttcaa aaatatgcaa agctgagtgc gagttttgag
    11041 tgtaagattc ttttaattct ttcactaaac ttgtgacttc tgcttcgctg agatttaaca
    11101 gtccgcttaa ttcttcatta ctgatccctt cctcgccaac aataaaaaga agggcttcaa
    11161 gttcacttaa tttcgtcatg ttcggtatct cctactgctg gataaagcat aattgattca
    11221 tagttttctt tttgttgcgc aatgatctgc ccttttttca tcaattctag caaagccata
    11281 aaagtagtga caacttcact tttagaaggt gactcaaaaa aagaatcgaa agtacagcca
    11341 tgcgcatttc tttttatacg ctcttcgata aataccattc gttcttcgat cgttgtgtca
    11401 tcgggcgtga tcgttttttc cactttttga gaaaatttct tacgttctaa gattttctgc
    11461 atggcacgga tcaaatcgat ttttttgact tgtcctcgct tcaacggctt ttcagcatcc
    11521 gctaacccat cgatgttcat aggttctttt gtgtaataaa gacttcgttc ctctgctttt
    11581 tctgtcagtc tttcagctgc atatttatat ttacggtatt ctaaaagctg agtgaccaat
    11641 tcttctcgag gatcttcttc ccataattct tcatcgtctg tgacgaattc ttggacagga
    11701 agcaatgtct tgcttttgat tgccatcaat gtagcagcca tcactaagta ttctcctgca
    11761 acagctagct cgagtgtttt cattgtatgg atataattca tatattgctc cgtgatcgta
    11821 gcgatcggta tatcatatat atcgatttcc atttgctgaa tcagatgaag cagcagatcg
    11881 agaggaccct cgaatacatc taatttaata ttgatctccg tcaactcatt cactctttct
    11941 tctttttctt tgctagccat tttccaatgt aaggctctgt gatttcagtt gcacggatgg
    12001 agacatccgg gtacttttcc tgtaattcat ctaacatttg aaagcccata ttctcaccgc
    12061 gataagacgg attcaaagaa atgtcatgga tgatcagctg atcgttcgtt gcccagtaaa
    12121 taccaataat cccttgaatg ttggttgaac cttctggcgt aaagaaaaaa agttcataat
    12181 cgtcttcaga ttcgtacgta tcaatctcct tcaacaaaga agttttacct gtcagtttct
    12241 tatggaaact caatagtccc atagcaattt tacggttttc tttccggtaa tgtgttaaca
    12301 tcttctcacc ttcctatgac taagcccttg gaaaatgctg cttataaaca tccgtcattc
    12361 ttttcttcgt gatatgtgta taaatctgag tcgtagagat atccgcgtga cctaacaatt
    12421 cctgtactgt ccgcaaatca gctccatttt ctagcagatg tgtggcaaag ctatgccgca
    12481 gcgtatgtgg tgttacttct ttgtaaatcc cagcctctcg tactaactgc ttcagatttt
    12541 tccagattcc ttgccgagaa agacctttcc catgattatt gacaaataca tgcatttcag
    12601 atgcgtcctt tacaagtaat ggacgtgctt cgtctaagta gcgttccagc cattggatcg
    12661 cataatcacc taaaggaatg atccgctctt tatctccttt tccaactgtc tgtaccagtc
    12721 caagagaaag atgaagatct cccaatttca atccaatcaa ttcactgacg cgcatcccag
    12781 tagcatacat gacttccaag attgcacgat ctcttatccc taacgttttt gtcgtatcag
    12841 gtgcttcaat cagcttttct acttctgtca gagtcaatgt acttggtaat ttctgtgctt
    12901 tcttcggcgt atcgatatgt tgcatcggat catgatcagt gatacgctct tgccgtaaaa
    12961 actgatggaa tcttctgaga cttgagatca tccgagtaac agttgctgtc gctttttttt
    13021 cttcatgcaa cttctccaga aaacggatga ctaaaaagcg atcgacatcc tgccatgaag
    13081 caattttctg ctcctgtaga aaatgcaagt actgttccaa atcacgttca tagctttgtc
    13141 gtgtattggc ggacaatccc cgttcaatat tcaagtagtg gagataatcg ttgatttgct
    13201 ctttcataat aatccccctc atgcctctct atcatatcag ataccgcttc attctgatag
    13261 tctttttcca taatctgagc aaagaaaaag ccgtctagac actcagacgg actggtttct
    13321 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
    13381 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
    13441 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
    13501 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
    13561 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
    13621 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
    13681 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
    13741 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
    13801 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
    13861 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
    13921 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
    13981 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
    14041 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
    14101 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
    14161 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
    14221 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
    14281 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
    14341 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
    14401 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
    14461 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
    14521 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
    14581 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
    14641 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
    14701 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
    14761 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
    14821 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
    14881 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
    14941 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
    15001 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
    15061 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
    15121 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
    15181 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
    15241 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
    15301 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
    15361 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
    15421 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
    15481 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
    15541 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
    15601 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
    15661 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
    15721 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
    15781 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
    15841 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
    15901 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
    15961 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
    16021 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
    16081 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
    16141 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
    16201 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
    16261 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
    16321 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
    16381 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
    16441 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
    16501 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
    16561 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
    16621 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
    16681 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
    16741 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
    16801 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
    16861 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
    16921 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
    16981 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
    17041 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
    17101 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
    17161 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
    17221 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
    17281 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
    17341 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
    17401 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
    17461 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
    17521 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
    17581 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
    17641 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
    17701 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
    17761 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
    17821 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
    17881 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
    17941 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
    18001 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
    18061 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
    18121 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
    18181 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
    18241 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
    18301 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
    18361 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
    18421 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
    18481 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
    18541 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
    18601 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
    18661 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
    18721 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
    18781 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
    18841 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
    18901 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
    18961 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
    19021 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
    19081 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
    19141 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
    19201 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
    19261 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
    19321 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
    19381 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
    19441 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
    19501 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
    19561 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
    19621 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
    19681 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
    19741 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
    19801 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
    19861 ctttttcatg agcagcaatc agtttccccg tagcagattc aggatttcgg acagctatag
    19921 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
    19981 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
    20041 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
    20101 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
    20161 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
    20221 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
    20281 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
    20341 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
    20401 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
    20461 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
    20521 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
    20581 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
    20641 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
    20701 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
    20761 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
    20821 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
    20881 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
    20941 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
    21001 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
    21061 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
    21121 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
    21181 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
    21241 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
    21301 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
    21361 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
    21421 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
    21481 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
    21541 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
    21601 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
    21661 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
    21721 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
    21781 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
    21841 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
    21901 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
    21961 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
    22021 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
    22081 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
    22141 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
    22201 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
    22261 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
    22321 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
    22381 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
    22441 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
    22501 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
    22561 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
    22621 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
    22681 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
    22741 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
    22801 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
    22861 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
    22921 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
    22981 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
    23041 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
    23101 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
    23161 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
    23221 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
    23281 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    23341 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    23401 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    23461 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    23521 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    23581 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    23641 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    23701 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    23761 ttgccttttt tccactattt gcgtactcct tctttcattt aattttattc atgattgaac
    23821 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    23881 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    23941 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    24001 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    24061 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    24121 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    24181 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    24241 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    24301 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    24361 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    24421 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    24481 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    24541 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    24601 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    24661 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    24721 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    24781 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    24841 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    24901 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    24961 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    25021 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    25081 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    25141 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    25201 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    25261 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    25321 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    25381 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    25441 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    25501 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    25561 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    25621 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    25681 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    25741 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    25801 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    25861 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    25921 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    25981 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    26041 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    26101 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    26161 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    26221 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    26281 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    26341 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    26401 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    26461 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    26521 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    26581 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    26641 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    26701 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    26761 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    26821 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    26881 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    26941 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    27001 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    27061 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    27121 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    27181 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    27241 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    27301 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    27361 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    27421 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    27481 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    27541 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    27601 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    27661 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    27721 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    27781 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    27841 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    27901 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    27961 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    28021 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    28081 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    28141 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    28201 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    28261 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    28321 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    28381 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    28441 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    28501 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    28561 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    28621 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    28681 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    28741 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    28801 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    28861 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    28921 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    28981 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    29041 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    29101 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    29161 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    29221 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    29281 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    29341 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    29401 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    29461 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    29521 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    29581 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    29641 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    29701 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    29761 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    29821 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    29881 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    29941 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    30001 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    30061 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    30121 taaaatttta ttagataaaa
//
LOCUS       DAZKMK010000023        29891 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000023 DAZKMK010000000
VERSION     DAZKMK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.023"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8V1_001074"
     CDS             85..624
                     /locus_tag="ACG8V1_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329181.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="ACG8V1_001075"
     CDS             1644..2813
                     /locus_tag="ACG8V1_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0329182.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="ACG8V1_001076"
     CDS             3286..3840
                     /locus_tag="ACG8V1_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0329183.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4450)
                     /locus_tag="ACG8V1_001077"
     CDS             complement(4013..4450)
                     /locus_tag="ACG8V1_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0329184.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5445
                     /locus_tag="ACG8V1_001078"
                     /pseudo
     CDS             4498..5445
                     /locus_tag="ACG8V1_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            5716..6151
                     /locus_tag="ACG8V1_001079"
                     /pseudo
     CDS             5716..6151
                     /locus_tag="ACG8V1_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            6167..7000
                     /locus_tag="ACG8V1_001080"
     CDS             6167..7000
                     /locus_tag="ACG8V1_001080"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0329185.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            7245..8270
                     /locus_tag="ACG8V1_001081"
     CDS             7245..8270
                     /locus_tag="ACG8V1_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0329186.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8408..8533
                     /locus_tag="ACG8V1_001082"
     CDS             8408..8533
                     /locus_tag="ACG8V1_001082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329187.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            8558..9673
                     /locus_tag="ACG8V1_001083"
                     /pseudo
     CDS             8558..9673
                     /locus_tag="ACG8V1_001083"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            9756..10652
                     /locus_tag="ACG8V1_001084"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="ACG8V1_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            10668..11492
                     /locus_tag="ACG8V1_001085"
     CDS             10668..11492
                     /locus_tag="ACG8V1_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329188.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="ACG8V1_001086"
     CDS             11524..12834
                     /locus_tag="ACG8V1_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0329189.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="ACG8V1_001087"
     CDS             12982..14241
                     /locus_tag="ACG8V1_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0329190.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="ACG8V1_001088"
     CDS             14251..15123
                     /locus_tag="ACG8V1_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329191.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="ACG8V1_001089"
     CDS             15135..15965
                     /locus_tag="ACG8V1_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329192.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="ACG8V1_001090"
     CDS             16005..18188
                     /locus_tag="ACG8V1_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0329193.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="ACG8V1_001091"
     CDS             18249..19469
                     /locus_tag="ACG8V1_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0329194.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="ACG8V1_001092"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="ACG8V1_001092"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0329195.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="ACG8V1_001093"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="ACG8V1_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="ACG8V1_001094"
     CDS             22431..22640
                     /locus_tag="ACG8V1_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0329196.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="ACG8V1_001095"
     CDS             22633..22782
                     /locus_tag="ACG8V1_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0329197.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="ACG8V1_001096"
     CDS             22936..23601
                     /locus_tag="ACG8V1_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0329198.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACG8V1_001097"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACG8V1_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0329199.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="ACG8V1_001098"
     CDS             25304..25537
                     /locus_tag="ACG8V1_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0329200.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="ACG8V1_001099"
     CDS             25534..25941
                     /locus_tag="ACG8V1_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0329201.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="ACG8V1_001100"
     CDS             complement(27078..27596)
                     /locus_tag="ACG8V1_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0329202.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27963..28211
                     /locus_tag="ACG8V1_001101"
     CDS             27963..28211
                     /locus_tag="ACG8V1_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329203.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            28714..29876
                     /locus_tag="ACG8V1_001102"
     CDS             join(28714..28990,28990..29876)
                     /locus_tag="ACG8V1_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0329204.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSSSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT        10301 a         4530 c         5492 g         9568 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc acttagcttg gataggctac aattaactag acagaaaaat taaggtgtgt
    28681 agactagaag aaaacatacc aggaggaatt tttatgtcta agagaacacg aagaactttt
    28741 tcacaagaat tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa
    28801 atcattcgag aatatgaact aacggcttca gcatttgaca aatgggtaaa gcaatctaaa
    28861 acgagtagtt cattcaaaga aaaagataat cttacgcctg aacaaaaaga attgttagaa
    28921 ctacgtaaaa gaaaccagca attagaaatg gaaaatgata ttttaaagca agcagcgctg
    28981 atattcggac gaagagacaa gtaatcgatg cgaataagca tctttaccct atatcagcga
    29041 tgtgcagaat attaggtcta tcacgtcagt cctattatta tcaatcaaaa ccaaagaaag
    29101 acgaatcaga acttgaagaa gtagtcgctg aagaatttat ccgcagccga aaggcctacg
    29161 gctcaagaaa aataaaaaaa gccttatcaa aacgaggcat tcagatcagc cgacgaaaaa
    29221 ttagtagaat catgaaaaat agaggattaa aatcgagcta tactgttgct tattttaaag
    29281 tacatcattc tacttgcaat gaagccaaaa cgacaaacgt attgaatcgt aaattcttaa
    29341 gagacaaccc attagaagcg atcgtaacag acttgactta tgtacgagtc gggaaaaaat
    29401 ggaattatgt ctgtttcatt ttggatctgt tcaatcgaga aattctcggc tattcttgtg
    29461 gagaacataa agatgccgtt ctagtaaaaa aagcatttag ccgtatcaaa caacctctga
    29521 cagaggttga gatttttcat actgatcgtg gaaaagagtt tgataaccaa gctattgatg
    29581 aattattaac aacttttgac atcaatcgat cattgagtca taaaggctgt ccttttgata
    29641 atgccgtagc tgaatcaact tataagtcgt tgaaagtaga atttgtctat caatacacat
    29701 ttgaaacctt acaacaattg gatttggagt tatttgacta tgtcaattgg tggaaccacc
    29761 ttcggttgca cggtacactt ggctacgaga caccggttgg ttaccgtaac cagagattgg
    29821 cgcagcgaat ccttgataat gagctcggat gtgctaacgc tagcgaggca gtctaacttt
    29881 aactgttagc t
//
LOCUS       DAZKMK010000024        28603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000024 DAZKMK010000000
VERSION     DAZKMK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.024"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACG8V1_001103"
     CDS             237..1877
                     /locus_tag="ACG8V1_001103"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0329205.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACG8V1_001104"
     CDS             2031..3353
                     /locus_tag="ACG8V1_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0329206.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACG8V1_001105"
     CDS             complement(3415..3846)
                     /locus_tag="ACG8V1_001105"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0329207.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACG8V1_001106"
     CDS             complement(3910..4746)
                     /locus_tag="ACG8V1_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0329208.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACG8V1_001107"
     CDS             complement(4936..5238)
                     /locus_tag="ACG8V1_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0329209.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8V1_001108"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8V1_001108"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0329210.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACG8V1_001109"
     CDS             complement(5692..5958)
                     /locus_tag="ACG8V1_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0329211.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACG8V1_001110"
     CDS             complement(6212..6937)
                     /locus_tag="ACG8V1_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0329212.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACG8V1_001111"
     tRNA            complement(7303..7373)
                     /locus_tag="ACG8V1_001111"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACG8V1_001112"
     CDS             7490..8242
                     /locus_tag="ACG8V1_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0329213.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACG8V1_001113"
     CDS             8258..8737
                     /locus_tag="ACG8V1_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0329214.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACG8V1_001114"
     CDS             complement(8988..10568)
                     /locus_tag="ACG8V1_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0329215.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACG8V1_001115"
     CDS             complement(10561..11304)
                     /locus_tag="ACG8V1_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329216.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="ACG8V1_001116"
     CDS             complement(11480..12256)
                     /locus_tag="ACG8V1_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0329217.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="ACG8V1_001117"
     CDS             complement(12504..13340)
                     /locus_tag="ACG8V1_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0329218.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="ACG8V1_001118"
     CDS             complement(13351..14280)
                     /locus_tag="ACG8V1_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0329219.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="ACG8V1_001119"
     CDS             14472..14825
                     /locus_tag="ACG8V1_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0329220.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="ACG8V1_001120"
     CDS             complement(14899..15555)
                     /locus_tag="ACG8V1_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0329221.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="ACG8V1_001121"
     CDS             15781..17715
                     /locus_tag="ACG8V1_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0329222.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="ACG8V1_001122"
     CDS             complement(17801..19024)
                     /locus_tag="ACG8V1_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0329223.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20889)
                     /locus_tag="ACG8V1_001123"
     CDS             complement(19018..20889)
                     /locus_tag="ACG8V1_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0329224.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="ACG8V1_001124"
     CDS             complement(20910..22028)
                     /locus_tag="ACG8V1_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0329225.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="ACG8V1_001125"
     CDS             complement(22111..22944)
                     /locus_tag="ACG8V1_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329226.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="ACG8V1_001126"
     CDS             complement(22937..23854)
                     /locus_tag="ACG8V1_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329227.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="ACG8V1_001127"
     CDS             complement(24081..25205)
                     /locus_tag="ACG8V1_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0329228.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="ACG8V1_001128"
     CDS             25388..26296
                     /locus_tag="ACG8V1_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0329229.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="ACG8V1_001129"
     CDS             26437..28050
                     /locus_tag="ACG8V1_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0329230.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DAZKMK010000025        27721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000025 DAZKMK010000000
VERSION     DAZKMK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.025"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG8V1_001130"
     CDS             <1..288
                     /locus_tag="ACG8V1_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329231.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACG8V1_001131"
     CDS             347..787
                     /locus_tag="ACG8V1_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329232.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACG8V1_001132"
     CDS             799..1467
                     /locus_tag="ACG8V1_001132"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0329233.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACG8V1_001133"
     CDS             1531..2154
                     /locus_tag="ACG8V1_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0329234.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACG8V1_001134"
     CDS             2210..2596
                     /locus_tag="ACG8V1_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0329235.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACG8V1_001135"
     CDS             2809..4200
                     /locus_tag="ACG8V1_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0329236.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="ACG8V1_001136"
     CDS             4811..5179
                     /locus_tag="ACG8V1_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0329237.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="ACG8V1_001137"
     CDS             5359..6300
                     /locus_tag="ACG8V1_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0329238.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="ACG8V1_001138"
     CDS             6369..7100
                     /locus_tag="ACG8V1_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0329239.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="ACG8V1_001139"
     CDS             7244..9424
                     /locus_tag="ACG8V1_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0329240.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="ACG8V1_001140"
     CDS             9444..9926
                     /locus_tag="ACG8V1_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0329241.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="ACG8V1_001141"
     CDS             9942..10703
                     /locus_tag="ACG8V1_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0329242.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /gene="murC"
                     /locus_tag="ACG8V1_001142"
     CDS             10834..12168
                     /gene="murC"
                     /locus_tag="ACG8V1_001142"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0329243.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="ACG8V1_001143"
     CDS             12475..12960
                     /locus_tag="ACG8V1_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0329244.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="ACG8V1_001144"
     CDS             12953..13636
                     /locus_tag="ACG8V1_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0329245.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="ACG8V1_001145"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="ACG8V1_001145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0329246.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="ACG8V1_001146"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="ACG8V1_001146"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0329247.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /gene="coaE"
                     /locus_tag="ACG8V1_001147"
     CDS             17252..17881
                     /gene="coaE"
                     /locus_tag="ACG8V1_001147"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0329248.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="ACG8V1_001148"
     CDS             17933..18265
                     /locus_tag="ACG8V1_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0329249.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG8V1_001149"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG8V1_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0329250.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="ACG8V1_001150"
     CDS             19091..20464
                     /locus_tag="ACG8V1_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HFQ0329251.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LYDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG8V1_001151"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG8V1_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0329252.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="ACG8V1_001152"
     CDS             21519..21959
                     /locus_tag="ACG8V1_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0329253.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22103..22804
                     /locus_tag="ACG8V1_001153"
     CDS             22103..22804
                     /locus_tag="ACG8V1_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0329254.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            22801..23922
                     /locus_tag="ACG8V1_001154"
     CDS             22801..23922
                     /locus_tag="ACG8V1_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0329255.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23937..25169
                     /gene="pepT"
                     /locus_tag="ACG8V1_001155"
     CDS             23937..25169
                     /gene="pepT"
                     /locus_tag="ACG8V1_001155"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0329256.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25301..26209
                     /locus_tag="ACG8V1_001156"
     CDS             25301..26209
                     /locus_tag="ACG8V1_001156"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0329257.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26244..26516
                     /locus_tag="ACG8V1_001157"
     CDS             26244..26516
                     /locus_tag="ACG8V1_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0329258.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26527..27573
                     /locus_tag="ACG8V1_001158"
     CDS             26527..27573
                     /locus_tag="ACG8V1_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0329259.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9325 a         4793 c         6034 g         7569 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtctttat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata
    22081 attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt
    22141 gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt
    22201 acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca
    22261 aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac
    22321 cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac
    22381 gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca
    22441 acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg
    22501 atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa
    22561 aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat
    22621 ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg
    22681 gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca
    22741 agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc
    22801 atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta
    22861 gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga
    22921 gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc
    22981 gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat
    23041 cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca
    23101 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
    23161 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
    23221 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
    23281 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
    23341 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
    23401 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
    23461 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
    23521 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
    23581 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
    23641 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
    23701 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
    23761 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
    23821 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
    23881 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
    23941 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
    24001 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
    24061 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
    24121 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
    24181 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
    24241 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
    24301 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
    24361 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
    24421 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
    24481 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
    24541 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
    24601 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
    24661 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
    24721 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
    24781 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
    24841 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
    24901 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
    24961 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
    25021 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
    25081 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
    25141 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
    25201 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
    25261 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
    25321 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
    25381 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
    25441 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
    25501 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
    25561 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
    25621 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
    25681 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
    25741 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
    25801 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
    25861 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
    25921 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
    25981 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
    26041 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
    26101 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
    26161 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
    26221 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc
    26281 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
    26341 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
    26401 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
    26461 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
    26521 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
    26581 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
    26641 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
    26701 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
    26761 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
    26821 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
    26881 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
    26941 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
    27001 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
    27061 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
    27121 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
    27181 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
    27241 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
    27301 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
    27361 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
    27421 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
    27481 tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa
    27541 gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat
    27601 caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat
    27661 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
    27721 a
//
LOCUS       DAZKMK010000026        27531 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000026 DAZKMK010000000
VERSION     DAZKMK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.026"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..542
                     /gene="nrdI"
                     /locus_tag="ACG8V1_001159"
     CDS             96..542
                     /gene="nrdI"
                     /locus_tag="ACG8V1_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0329260.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(607..2589)
                     /locus_tag="ACG8V1_001160"
     CDS             complement(607..2589)
                     /locus_tag="ACG8V1_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0329261.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2603..3499)
                     /gene="murQ"
                     /locus_tag="ACG8V1_001161"
     CDS             complement(2603..3499)
                     /gene="murQ"
                     /locus_tag="ACG8V1_001161"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0329262.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(3502..4563)
                     /locus_tag="ACG8V1_001162"
     CDS             complement(3502..4563)
                     /locus_tag="ACG8V1_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0329263.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(4774..5301)
                     /locus_tag="ACG8V1_001163"
     CDS             complement(4774..5301)
                     /locus_tag="ACG8V1_001163"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0329264.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(5368..6870)
                     /gene="eat(A)"
                     /locus_tag="ACG8V1_001164"
     CDS             complement(5368..6870)
                     /gene="eat(A)"
                     /locus_tag="ACG8V1_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0329265.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(7128..8249)
                     /locus_tag="ACG8V1_001165"
     CDS             complement(7128..8249)
                     /locus_tag="ACG8V1_001165"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0329266.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8375..9316
                     /locus_tag="ACG8V1_001166"
     CDS             8375..9316
                     /locus_tag="ACG8V1_001166"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0329267.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(9466..10362)
                     /locus_tag="ACG8V1_001167"
     CDS             complement(9466..10362)
                     /locus_tag="ACG8V1_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0329268.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            10631..11971
                     /locus_tag="ACG8V1_001168"
     CDS             10631..11971
                     /locus_tag="ACG8V1_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0329269.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12189..12632
                     /locus_tag="ACG8V1_001169"
     CDS             12189..12632
                     /locus_tag="ACG8V1_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0329270.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13136..14554)
                     /gene="nhaC"
                     /locus_tag="ACG8V1_001170"
     CDS             complement(13136..14554)
                     /gene="nhaC"
                     /locus_tag="ACG8V1_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0329271.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(14957..16180)
                     /locus_tag="ACG8V1_001171"
     CDS             complement(14957..16180)
                     /locus_tag="ACG8V1_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0329272.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16211..17050)
                     /locus_tag="ACG8V1_001172"
     CDS             complement(16211..17050)
                     /locus_tag="ACG8V1_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0329273.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17067..17747)
                     /locus_tag="ACG8V1_001173"
     CDS             complement(17067..17747)
                     /locus_tag="ACG8V1_001173"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0329274.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(17749..18822)
                     /locus_tag="ACG8V1_001174"
     CDS             complement(17749..18822)
                     /locus_tag="ACG8V1_001174"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329275.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(18878..19116)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(19159..19392)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19469..20140)
                     /locus_tag="ACG8V1_001175"
     CDS             complement(19469..20140)
                     /locus_tag="ACG8V1_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0329276.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(20227..22875)
                     /gene="mgtA"
                     /locus_tag="ACG8V1_001176"
     CDS             complement(20227..22875)
                     /gene="mgtA"
                     /locus_tag="ACG8V1_001176"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0329277.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(23217..23391)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            23564..24001
                     /gene="ndk"
                     /locus_tag="ACG8V1_001177"
     CDS             23564..24001
                     /gene="ndk"
                     /locus_tag="ACG8V1_001177"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0329278.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(24023..24889)
                     /locus_tag="ACG8V1_001178"
     CDS             complement(24023..24889)
                     /locus_tag="ACG8V1_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0329279.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(25049..27142)
                     /locus_tag="ACG8V1_001179"
     CDS             complement(25049..27142)
                     /locus_tag="ACG8V1_001179"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0329280.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         7875 a         5793 c         4850 g         9013 t
ORIGIN      
        1 ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac ttaaaaaaga
       61 tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat atatcgattt
      121 caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa atgcatgcag
      181 aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt gattttacga
      241 aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga aatggtttag
      301 ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa catgaagaca
      361 atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat aatcagtatt
      421 gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca gattatgaat
      481 tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa aacaataaat
      541 aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc tattgtttct
      601 atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac ctgttttttc
      661 caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg atgtttcttt
      721 gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg
      781 ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg tgttgattcc
      841 catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg
      901 gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt ctggttctac
      961 aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt tatctaaaga
     1021 aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg aagtttgagg
     1081 tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt cttctttttc
     1141 taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag cataagctgc
     1201 caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga tcaaagcggc
     1261 tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca ctgccccgcc
     1321 gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg ttactccgta
     1381 aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga tcatttctgt
     1441 taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca cttgtcctgc
     1501 accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa tcatttgcac
     1561 atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga ataatgtgcc
     1621 tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc caccgaccat
     1681 gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca gaccgatggc
     1741 aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta ctttatgcag
     1801 ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga tcccaccttg
     1861 tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg gattcatccc
     1921 tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac caaaaacttg
     1981 tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt tcaagatgtt
     2041 catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg tgactaagtt
     2101 tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag gaatcattgg
     2161 cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt tttgtttcga
     2221 tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc cagatgctga
     2281 tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca cgcctgccaa
     2341 atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga cttgaagctg
     2401 ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga catttacaag
     2461 atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc tttctacatt
     2521 gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag tcagttcgtt
     2581 ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt gcttcagcta
     2641 attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata gccagcttaa
     2701 cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct tctgtcgctt
     2761 gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg acatcgacca
     2821 tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc atattcaaga
     2881 taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat tctggacctg
     2941 catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa
     3001 tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc acatatggtg
     3061 tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc agcttgatta
     3121 gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca gtcattgctt
     3181 cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat gtcggtacgc
     3241 attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata taaatcagac
     3301 gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt
     3361 ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga acggcttgag
     3421 aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt gttaggttct
     3481 ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat agttcatacc
     3541 agctttgatt tggttgatca atggtagctc ttctttgata actttagcta ctacattcac
     3601 tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat agcgcaaata
     3661 cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc ttgctgggat
     3721 aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg catcgtcttg
     3781 ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag gttccgtatg
     3841 gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt tttctgacag
     3901 acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta gagcgcctgc
     3961 aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata gtgggcctct
     4021 caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt tgagccattg
     4081 attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat aataattatg
     4141 ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga tatctttagg
     4201 cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg caatctgctg
     4261 attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca aaggcgttag
     4321 atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga ccatcaattc
     4381 tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat atgccgaaac
     4441 atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg atgccatttg
     4501 gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg aaaagccaaa
     4561 catattatcc ctctttttct gtatccgtct tcatttctat gactagagta tatatctaaa
     4621 gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa ggttgagttt
     4681 atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt
     4741 gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt
     4801 atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac
     4861 tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc
     4921 tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg
     4981 tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata
     5041 atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc
     5101 agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat
     5161 tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc
     5221 agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc
     5281 gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat
     5341 accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga
     5401 tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa
     5461 gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca
     5521 tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc
     5581 catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag
     5641 gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc
     5701 tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt
     5761 ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc
     5821 tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg
     5881 ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg
     5941 gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt
     6001 ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt
     6061 ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa
     6121 tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc
     6181 tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa
     6241 ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag
     6301 ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg
     6361 gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc
     6421 agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa
     6481 taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca
     6541 taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa
     6601 atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg
     6661 gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag
     6721 aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg
     6781 tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa
     6841 tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc
     6901 ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa agacccatac
     6961 atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta
     7021 cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt
     7081 gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg
     7141 atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg
     7201 tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga
     7261 aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt
     7321 cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga
     7381 tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca
     7441 gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct
     7501 ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa
     7561 tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct
     7621 attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg
     7681 gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa
     7741 tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc
     7801 aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata
     7861 atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa
     7921 gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca
     7981 gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc
     8041 tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag
     8101 attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta
     8161 cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta
     8221 aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac
     8281 actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta
     8341 aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg
     8401 agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat
     8461 cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa
     8521 cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa
     8581 cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg
     8641 ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg
     8701 tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat
     8761 gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga
     8821 actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca
     8881 agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc
     8941 ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact
     9001 aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat
     9061 cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac
     9121 ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa
     9181 ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata
     9241 tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat
     9301 ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct
     9361 ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt
     9421 gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac
     9481 agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt
     9541 tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt
     9601 atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg
     9661 acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata
     9721 catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat
     9781 cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc
     9841 attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg
     9901 cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt
     9961 ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag
    10021 cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc
    10081 ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac
    10141 aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc
    10201 taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga
    10261 ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag
    10321 tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc
    10381 tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct
    10441 atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat
    10501 taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg ttatcattag
    10561 tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat ttaaaagaaa
    10621 gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac atatcaccat
    10681 gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg
    10741 gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt tatatatcgt
    10801 gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg gattatctgg
    10861 tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg tctactggtc
    10921 tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg
    10981 gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa ctacgatcat
    11041 caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt ttgctaaaat
    11101 cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc aaatgttggg
    11161 cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta ctcctcatgg
    11221 tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg gttctgaact
    11281 gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta aagcaatcaa
    11341 aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc ttttgattct
    11401 ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa
    11461 aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg ctttgttctc
    11521 atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt tgaaggatcg
    11581 aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc aacgtgctgt
    11641 tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg tcggtgacaa
    11701 attatttggc tatctggcag gttccctttc gtatacagta ttattgatct ggatcattat
    11761 cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata aaggaatcag
    11821 cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca cgaactccat
    11881 tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa
    11941 gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat tttatttatg
    12001 tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg caactccttt
    12061 ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa
    12121 ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt
    12181 tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca acggtagtta
    12241 tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc agtcgtacac
    12301 gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg ttgttcatgt
    12361 ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt cttcttgttt
    12421 ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca gcttggatga
    12481 aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct gatttaaaag
    12541 atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gagatcgaca
    12601 aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata aaaaaagagg
    12661 atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga acgccgtgaa
    12721 taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    12781 ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
    12841 tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa atggaaaaat
    12901 agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt
    12961 ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat
    13021 ggctttccac atttttgtat aaatttggct taataccgag aaactggctt ttgaataccg
    13081 tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta tgtttttatt
    13141 gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt tttgagattc
    13201 cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg aaaaatacga
    13261 atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt ccggcaattc
    13321 cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta cctaatacag
    13381 ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc agaataacag
    13441 ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat gttgtaaaaa
    13501 tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt gagatcaagc
    13561 caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac acagtaggca
    13621 tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt ccagtatttg
    13681 aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt gggttttgaa
    13741 taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt gccggcacct
    13801 ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt gaaatagtaa
    13861 agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg gctttacttg
    13921 tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca
    13981 tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct agatttgttg
    14041 attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata gctccaacaa
    14101 ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg
    14161 acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca acaaatacag
    14221 aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga gttggaatga
    14281 tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata aagattggga
    14341 tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt
    14401 ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata ttaggtgaga
    14461 cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat acaagtgttg
    14521 cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt ttagttgttc
    14581 ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc gcgattcctc
    14641 ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc ggtaccacct
    14701 taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat ggactttcca
    14761 tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct gaacaaaaca
    14821 cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat aagatcattt
    14881 gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata actttcacac
    14941 acaagattat tttttactag aaagtagcgc cctcttgttt ttttcttgct actatttctt
    15001 gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca agagctgtat
    15061 tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt ttagcagcag
    15121 ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc gtaggctgga
    15181 ttgttggtat gacttggctg acatttccta catcagaaga accgagactt gttctttgtt
    15241 ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag aataagtcat
    15301 caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca aactcaaaag
    15361 tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca tatactttat
    15421 ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga
    15481 cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct tttggcaatt
    15541 gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa gcatttactc
    15601 ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg tcgactggat
    15661 cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg cataacgcta
    15721 catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat ccgttctcgc
    15781 ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca tcaatgaatt
    15841 gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc ccgcatgcat
    15901 gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga ccgggtttat
    15961 tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt tctacccaaa
    16021 acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca tagttactta
    16081 cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa cgatattgga
    16141 ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc tcctatgcta
    16201 gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat ccttttgatg
    16261 ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt ttttgatacg
    16321 ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga cgtgcatggt
    16381 catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca tctacagcta
    16441 caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt tgggaagcat
    16501 ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca gtgggcgctt
    16561 gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga agagctctgc
    16621 cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata tcagctacat
    16681 ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg atcgatacta
    16741 aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca
    16801 aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta aatttcacgt
    16861 attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat acatctgtat
    16921 cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta tctgcttttc
    16981 cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca aaacgaataa
    17041 tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag ttgattactg
    17101 ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat cgttacatct
    17161 gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc tgcacctact
    17221 gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga gacccgaatg
    17281 ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg actggttccc
    17341 atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt ctcgatttga
    17401 cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt cgcaccaatc
    17461 gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat aatgaaaggg
    17521 atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg gttagctagt
    17581 aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat catcccgcca
    17641 atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac aaattcatcc
    17701 ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt atcttcctcc
    17761 ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta tttttacacc
    17821 agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag ctaaagtaac
    17881 gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata aaatattcgc
    17941 tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct gatttccaat
    18001 ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag aatgggcaat
    18061 gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt tggtcaattc
    18121 ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc ttccatcttc
    18181 cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac
    18241 gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct gaagggtcgt
    18301 tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt cattcgccaa
    18361 agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag gataagcaga
    18421 tgctttttct tccaaaccga caagttccag tagttcattc actttttgac gacgttcctg
    18481 tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc
    18541 cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt
    18601 gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg ttggtttctg
    18661 cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac cgacgattcc
    18721 ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc
    18781 tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc ctccttatct
    18841 aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt
    18901 taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg atcatactct
    18961 agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg aattgctcga
    19021 tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc tctgtcaaag
    19081 gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa gtccttttac
    19141 taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt
    19201 tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac
    19261 agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt
    19321 acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta ctttcctttt
    19381 caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg aaaagtcaag
    19441 tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt aacatttcaa
    19501 taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg
    19561 tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag
    19621 cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct tctaatcctg
    19681 ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac acttcgtctc
    19741 cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata tctgttgctc
    19801 ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt aagtctgcca
    19861 atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt tgataaaaat
    19921 attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct gctttcacta
    19981 aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt tcttttggga
    20041 tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt ttttctgtgt
    20101 caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta atcctttcca
    20161 aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct
    20221 tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag
    20281 cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag gaagtggcat
    20341 caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa taattcctaa
    20401 tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta ctttcggtgt
    20461 acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg
    20521 gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac ttccgcccac
    20581 tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa atatcgaact
    20641 tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt tttttggctc
    20701 gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa catcatagat
    20761 cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga acgtacttgc
    20821 aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga tatatttcat
    20881 gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt
    20941 ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat ctacagaaat
    21001 accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta aaaatccgac
    21061 agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag gattcaattt
    21121 tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca tttccgagat
    21181 tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca ctgaacgtgt
    21241 aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt tcagagcttt
    21301 caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac caatcaaaac
    21361 catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg tcttttgtgc
    21421 gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa gtacggcttc
    21481 ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt tagaaatgtt
    21541 cagcatttct tcaatcgcac cttttgtgat catttgtgtt ttaccagctg gatcagcaac
    21601 cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt tttgataatt
    21661 gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat ctaacaaatt
    21721 tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac gatcatcttc
    21781 atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca atgtccccgt
    21841 tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca aatttttgat
    21901 cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat
    21961 tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa aagcttctag
    22021 ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca atgacataaa
    22081 tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt gaattggttt
    22141 ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag aaacaatgat
    22201 tccttccgca cttccactga tgacattact tcccataaag acaagatttt cgtaacttgt
    22261 ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc
    22321 tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca tatccgctgg
    22381 tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat
    22441 ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg atttcagttt
    22501 ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc tgacgataac
    22561 catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg ctaatacata
    22621 ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta
    22681 tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc
    22741 gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg atgcatggaa
    22801 tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt tttgtgtatc
    22861 tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt
    22921 ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct gagaacagat
    22981 agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt catccatttg
    23041 ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt
    23101 atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg cggcaacaaa
    23161 aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa aaagcttctc
    23221 agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct gcttttagct
    23281 accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt
    23341 tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat tcggttttcc
    23401 ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact
    23461 ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc tcattttcca
    23521 cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa gaacacttgt
    23581 tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc aacgttttga
    23641 agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg aattagcaaa
    23701 agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg attacatgac
    23761 ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa
    23821 gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag gagactacgc
    23881 gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga
    23941 gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg
    24001 atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc agttttttct
    24061 ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag aattcttggt
    24121 agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa taatctgcca
    24181 tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt ttcatatctt
    24241 cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa gtcaggttca
    24301 ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt cgcattcttt
    24361 ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa ggaaaatcag
    24421 ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat tcttcaatga
    24481 ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt ttttcgacca
    24541 tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc ctacggttat
    24601 tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc attcgtcttt
    24661 ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat tctgcaagtg
    24721 tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt ttcactgaat
    24781 ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat tgggactgtg
    24841 tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata atagttctcc
    24901 tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga aaaatacacg
    24961 aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg
    25021 tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt tcaacgtctg
    25081 ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa aagacacttg
    25141 tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca tttttatagt
    25201 catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt
    25261 cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga
    25321 tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag
    25381 cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga aaagcaacta
    25441 cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga
    25501 ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat tcatgattga
    25561 ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt
    25621 tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa tctttattat
    25681 ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac
    25741 cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa tgtgccgttc
    25801 gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt
    25861 catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt
    25921 gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac
    25981 ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca taacctaagt
    26041 ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa acagcgcttg
    26101 tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg tctgacccaa
    26161 gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac atattttcag
    26221 catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg atcgttgctt
    26281 gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc ttcatattaa
    26341 tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct ttagcgatcc
    26401 caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa acttcatcaa
    26461 tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat
    26521 aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca aatgttcgct
    26581 gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa ataagccctg
    26641 aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg ataggtattt
    26701 tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc cagtagatga
    26761 catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa
    26821 agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca
    26881 agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac gctggctttg
    26941 gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt gggttgatcc
    27001 acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa
    27061 tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc
    27121 acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt
    27181 gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc
    27241 taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag cgattaaact
    27301 aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa aagccagttt
    27361 ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct ccttatttct
    27421 tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg ccaactcctc
    27481 ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt g
//
LOCUS       DAZKMK010000027        25733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000027 DAZKMK010000000
VERSION     DAZKMK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.027"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..2228
                     /locus_tag="ACG8V1_001180"
     CDS             168..2228
                     /locus_tag="ACG8V1_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0329281.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2311..2880
                     /locus_tag="ACG8V1_001181"
     CDS             2311..2880
                     /locus_tag="ACG8V1_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0329282.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3055..3861)
                     /locus_tag="ACG8V1_001182"
     CDS             complement(3055..3861)
                     /locus_tag="ACG8V1_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329283.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3999..5513
                     /locus_tag="ACG8V1_001183"
     CDS             3999..5513
                     /locus_tag="ACG8V1_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0329284.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5916..6899)
                     /locus_tag="ACG8V1_001184"
     CDS             complement(5916..6899)
                     /locus_tag="ACG8V1_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0329285.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6904..7551)
                     /locus_tag="ACG8V1_001185"
     CDS             complement(6904..7551)
                     /locus_tag="ACG8V1_001185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0329286.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7636..8268)
                     /locus_tag="ACG8V1_001186"
     CDS             complement(7636..8268)
                     /locus_tag="ACG8V1_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0329287.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="ACG8V1_001187"
     CDS             complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="ACG8V1_001187"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0329288.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8832..9569)
                     /locus_tag="ACG8V1_001188"
     CDS             complement(8832..9569)
                     /locus_tag="ACG8V1_001188"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0329289.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9566..10555)
                     /locus_tag="ACG8V1_001189"
     CDS             complement(9566..10555)
                     /locus_tag="ACG8V1_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0329290.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10813..11355
                     /locus_tag="ACG8V1_001190"
     CDS             10813..11355
                     /locus_tag="ACG8V1_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0329291.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11372..12457
                     /locus_tag="ACG8V1_001191"
     CDS             11372..12457
                     /locus_tag="ACG8V1_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329292.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12469..13275
                     /locus_tag="ACG8V1_001192"
     CDS             12469..13275
                     /locus_tag="ACG8V1_001192"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0329293.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13276..14103
                     /locus_tag="ACG8V1_001193"
     CDS             13276..14103
                     /locus_tag="ACG8V1_001193"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0329294.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14100..15173
                     /locus_tag="ACG8V1_001194"
     CDS             14100..15173
                     /locus_tag="ACG8V1_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0329295.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15601..18297
                     /locus_tag="ACG8V1_001195"
     CDS             15601..18297
                     /locus_tag="ACG8V1_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0329296.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18362..18862
                     /locus_tag="ACG8V1_001196"
     CDS             18362..18862
                     /locus_tag="ACG8V1_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0329297.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18874..19368
                     /locus_tag="ACG8V1_001197"
     CDS             18874..19368
                     /locus_tag="ACG8V1_001197"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0329298.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19389..20198
                     /locus_tag="ACG8V1_001198"
     CDS             19389..20198
                     /locus_tag="ACG8V1_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0329299.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20195..21016
                     /locus_tag="ACG8V1_001199"
     CDS             20195..21016
                     /locus_tag="ACG8V1_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0329300.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21067..23166
                     /locus_tag="ACG8V1_001200"
     CDS             21067..23166
                     /locus_tag="ACG8V1_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0329301.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23163..24281
                     /locus_tag="ACG8V1_001201"
     CDS             23163..24281
                     /locus_tag="ACG8V1_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0329302.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24320..25198
                     /locus_tag="ACG8V1_001202"
     CDS             24320..25198
                     /locus_tag="ACG8V1_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0329303.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25344..25541
                     /locus_tag="ACG8V1_001203"
     CDS             25344..25541
                     /locus_tag="ACG8V1_001203"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0329304.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25525..>25733
                     /locus_tag="ACG8V1_001204"
     CDS             25525..>25733
                     /locus_tag="ACG8V1_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329305.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSI"
BASE COUNT         8142 a         4471 c         5430 g         7690 t
ORIGIN      
        1 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
       61 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
      121 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
      181 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
      241 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
      301 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
      361 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
      421 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
      481 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
      541 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
      601 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
      661 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
      721 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
      781 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
      841 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
      901 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
      961 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     1021 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     1081 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     1141 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     1201 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     1261 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     1321 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     1381 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     1441 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     1501 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     1561 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     1621 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     1681 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     1741 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     1801 ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     1861 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     1921 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     1981 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     2041 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     2101 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     2161 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     2221 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     2281 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     2341 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     2401 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     2461 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     2521 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     2581 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     2641 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     2701 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     2761 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     2821 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     2881 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     2941 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     3001 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     3061 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     3121 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     3181 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     3241 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     3301 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     3361 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     3421 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     3481 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     3541 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     3601 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     3661 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     3721 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     3781 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     3841 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     3901 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     3961 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     4021 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     4081 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     4141 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     4201 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     4261 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     4321 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     4381 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     4441 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     4501 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     4561 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     4621 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     4681 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     4741 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     4801 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     4861 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     4921 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     4981 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     5041 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     5101 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     5161 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     5221 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     5281 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     5341 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     5401 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     5461 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     5521 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     5581 gaacactgtt tagtgaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     5641 caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
     5701 ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac acttttgtct
     5761 cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa ataagctgaa
     5821 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     5881 cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt caaaatgatt
     5941 tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc gctgaattgg
     6001 aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc agctttttgc
     6061 ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa taatgtacca
     6121 agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac gatggtccca
     6181 cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa attcttttcc
     6241 cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc agtgattttt
     6301 cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc accaagttct
     6361 ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc gtgagaaagc
     6421 tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg gatcaagaca
     6481 gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt caaaaaaact
     6541 tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat tgcacgatcg
     6601 gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta tacttttgtc
     6661 ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc tacttcgact
     6721 cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat atctgttcga
     6781 ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt aggaacaggc
     6841 acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat ggcacgcatg
     6901 gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc taccttcaga
     6961 aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa gtacagcagc
     7021 aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta attgaatgga
     7081 agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat ctaagttagt
     7141 tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca tggactcagg
     7201 ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag agacatgcgc
     7261 attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca gaagattctt
     7321 tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt cggtcatttt
     7381 ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt ttactaatgt
     7441 ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag ctaaatcttt
     7501 tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca tttggatcat
     7561 cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa aatcagagat
     7621 cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta acaagacatt
     7681 gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac ttgtaaaaat
     7741 aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt tggcataatc
     7801 ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca atgaaccgga
     7861 aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt tgcgcagacc
     7921 tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg cgatcagcaa
     7981 ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa agacaaaaga
     8041 caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg taccgccaag
     8101 aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa tatgcatggt
     8161 tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta ataaaatcaa
     8221 aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa gtactccttt
     8281 tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg tctgaatatc
     8341 agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg gttcaatttc
     8401 ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt tttgatacat
     8461 attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg ccattgcgac
     8521 tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt ttgctgtagc
     8581 aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga ttacctgcgg
     8641 atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa
     8701 tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg cacctaattt
     8761 gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag taacgcctag
     8821 taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa tgcagcaatc
     8881 tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata gccgtggtgt
     8941 gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt gttatttatc
     9001 agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa tagtttgaaa
     9061 ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt tggtgttttc
     9121 ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc
     9181 tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt gggtaggttt
     9241 tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac tgccatactg
     9301 tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa aaataagtcc
     9361 agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt cagtgcttgt
     9421 ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc agctaatgct
     9481 tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat tttttcagtc
     9541 gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact agtgttcttc
     9601 cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc agagtaatct
     9661 cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat aaagaaagac
     9721 gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt cgcagaataa
     9781 aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa gctactgcac
     9841 cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact gtgtcgatga
     9901 catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc
     9961 gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct tttctggaaa
    10021 aggtaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta ctgcctacac
    10081 cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga cggattaaag
    10141 cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg
    10201 tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat tggctataac
    10261 cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct tcttttgaaa
    10321 aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga gtcatcggat
    10381 atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa ttgacatcag
    10441 aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc agatcactga
    10501 ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg
    10561 agacactcct tttaaaattt tcttttatca taccataaag cctactcttt tattcttgtt
    10621 cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac tagaatttat
    10681 tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aagggttgcc tttttcaata
    10741 aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga aaacttcgat
    10801 tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg
    10861 acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc ccagttggaa
    10921 cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg
    10981 actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa
    11041 gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct cgtggctgat
    11101 tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg agaatataag
    11161 acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga attagagtta
    11221 agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca
    11281 aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg
    11341 caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt catttgatca
    11401 agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg agatcgaaca
    11461 aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga tcctacgtat
    11521 tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga aacggttgaa
    11581 tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg cactttttcc
    11641 tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa tgtccaaatc
    11701 agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa
    11761 acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg cacgagcaat
    11821 tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg atttgaaact
    11881 acgtacagat atgcagtatg aactgcggga tctgcaacaa cgattaggaa tcacgtttat
    11941 ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa
    12001 taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc caatcaatca
    12061 tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga tcgaagataa
    12121 tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc gtctaaatga
    12181 accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag ataaaggaaa
    12241 actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta
    12301 tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa
    12361 agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg agtctgaaga
    12421 agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat gaaaacaatg
    12481 cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat cgcgcctgta
    12541 ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct gaataattat
    12601 ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt atggtatgca
    12661 tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt tttaacaaaa
    12721 ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt gaatctgctg
    12781 cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt
    12841 gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt tctgttcgtg
    12901 gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt ggaagagatg
    12961 aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga gaccttccga
    13021 cgagtgattt ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc agtatttatt
    13081 ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt gatcacttta
    13141 ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg ttctacaatc
    13201 ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa
    13261 ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt
    13321 ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga aggaggaacg
    13381 atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga agatacacgt
    13441 ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt gatcgccacg
    13501 atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg tgcacggaca
    13561 gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat cggtgctagt
    13621 ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt attgctttcc
    13681 catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg
    13741 aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca agtagtcaaa
    13801 aatatcattt tgccttatct ttctccagga atcatagctg gatattttat ggcatttacc
    13861 tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt tacgacgctc
    13921 tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt
    13981 gcgttagttt tcttgttctc catgatattg gtcattggat actactttat tagtaaagaa
    14041 aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc acggctgcga
    14101 tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg ctgctgcttg
    14161 gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt accatctata
    14221 actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa tatggataca
    14281 aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg
    14341 ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa
    14401 aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt gatccacgat
    14461 ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt tggggaactt
    14521 tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat tgggatgatt
    14581 tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga gaagttatgg
    14641 ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca cagctgacag
    14701 aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg gcagatgaga
    14761 tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct ggcgaagctg
    14821 ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa ggatccaatc
    14881 tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt
    14941 tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat attggttatt
    15001 ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg gacgaacaat
    15061 tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta ggatcgaaat
    15121 acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga taatggcaaa
    15181 actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat
    15241 ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg tgttatttta
    15301 ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta aaatagggcc
    15361 cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt aattggaaac
    15421 tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat gaatccagtt
    15481 caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta aattggtaga
    15541 taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa
    15601 ttggaataca aatatagaat agacatattg cagccgatag tttattatgc ttttagacac
    15661 attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga tgagctgata
    15721 cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc
    15781 tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa ggtcgagaaa
    15841 ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt agataatacg
    15901 gagcaaataa acgaagcctt tcattctgta gttggtcatg acggaagtct gagagaggtc
    15961 attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt attaataaat
    16021 ggagccacgg gtgttggaaa aagtttttta gccacaaaaa tttttgaata cgctatccat
    16081 gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa actgtgccga ttatgcggac
    16141 aatcccgaac tgctttcggc aacactgttt ggatacaaga agggggcctt tacgggggct
    16201 gaaaaggata ccgatggttt attggcaacg gccaacaacg gctatctttt tctcgatgag
    16261 gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc tatttattga tactggaaac
    16321 tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt tattttcgcc
    16381 acgacggaaa aaggtgaaaa ttatttatta gacacctttg atcggcgtat tcaaattagt
    16441 gttatgttgc caacttttga agatcgcccc attcgagaac gattagagct gattcaatta
    16501 ttttttcaaa atgaagcgga tgttttacaa aaagatatta ttgtttcaag agaagccttg
    16561 tctttaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa tatcattaaa
    16621 atcagttgtg cggatgtgta tagtcgaaca caagaagaac cattaacgat tgggaaaaat
    16681 gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc gattgctagt
    16741 ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt tgattatgat
    16801 tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga aaatttttct
    16861 tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc ttttttatca
    16921 atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt tttaggaaaa
    16981 acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc ctatgtcatt
    17041 tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc aaatctacct
    17101 cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc agaaaaggac
    17161 gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga tgaatccatc
    17221 gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat tcaagcagta
    17281 gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt agagagtaat
    17341 gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt
    17401 ctagtgctga tcgtagatat gggctcttta aatcaaattt acacacagat caagaacgat
    17461 ttaaaagggg agttacttct tctgaataat ttgacgactt ccattgcact tgatattggc
    17521 ttgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc agacaagtat
    17581 tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacattat catttcctgt
    17641 atgtcgggtt tggggatttc ggacaaattg aaagatgttt ttcgtcatca tttatcggat
    17701 gaatccatcg aagttttcac gaaggactac agagaactac gtgaattaat cgaccaaaat
    17761 gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct tccacgttcc
    17821 ttttcaattc ctaatattaa tgtttatgac atcttagacg gtgatggagc agccaatttg
    17881 gagcatattt tgagtcctga aatgaagccg gcagaattta accgcttgat tcaggagcta
    17941 gtaaaattct tttcgttgga aggaattgcg gaccgtttga actttctgaa cccaacgatc
    18001 gtgatgagtg aagtggaaac agtcatttct aaatatgaaa attactatca tttcacttta
    18061 aatggccgtg taaaactaaa tctctatatg catacggcat tgatgatgga acgtttattc
    18121 ttggctcgga cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa
    18181 ttttattatg tttctagaag tatttttcaa tcaatggaga tgaaatataa ttttaaagtc
    18241 aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca aaaataaagt
    18301 gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca ctttttttaa tgtgaattaa
    18361 cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt gcttatataa aagtgaacag
    18421 aaagaaagga attaaccata tgaagaaaat tctaatagcg actcacggtc acttagcttc
    18481 aggctttctt agcagtattc aattactgac ggggaaggtt caagagatta cggtgattaa
    18541 tgcttatgtg gatgagtgtg attttgaaca ggagttgaat gtatttgtaa accaggtgaa
    18601 cgagcaggat cagatttttg tttttaccga tttatttggc ggaagtgtca atcagaagat
    18661 taccaaaacc tttttggaga aagagattgc tgtaactatt ttgtctggct ttaatctgcc
    18721 aattctgctc gaaatagtat tggcaaccaa ggaattaaca aaggaggata ttcaggaact
    18781 ggtaaataaa tgccaacagg aattaaagat gaccattctt gatgaggtac atgtagtaaa
    18841 tgaagaggaa gatttttttt aggaggaaga aaaatgatta aagcagtgcg aattgatgaa
    18901 cgattaattc atggacaagt agcgatgact tggacaaaga gtctaaattt aaccgggtta
    18961 gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa agatgacatt gaagatggct
    19021 gcaccatcga atgtcaaagt cattattaaa acagttgatg acgcgattca tttgttaaaa
    19081 gatccgcggg cgaaggacat gcgcttgatg gttttagtcc ctaatgtcaa agatgcggta
    19141 aaagttgcga aagctttccc aacggaaatc gaattaatga acgtggggaa tgctgggaaa
    19201 atgacaccgc catcggaaga taagaaaacc ttatcaaagg aagtgatgtt aacgacgtca
    19261 gaaattcaag acttaaaaga attagttgaa ctttatccag ataccgtttt tcagcctaca
    19321 ccggcgatgg aaaaacgttc agctatctct attttgagtg atttttagaa aaaggaagga
    19381 gaaaaattat gtatgatgca tttgtagtcg ctttggccgt ttttattgga gttgctggac
    19441 atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt tgttgctccg ttagcaggag
    19501 cgttgatggg ggatttgcag acggggttga ttgtcggggc gtcactagaa gccatcttta
    19561 tgggtgtcgt caatatcgga atctctagta cggcggagcc ttctttggct gctgggctgg
    19621 ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc gattattccc attgtctttc
    19681 cactagcagt attaggatta cagttaatga atatgatttt ttcttttgta tgtggaccga
    19741 tggctgctaa atttttcaaa tttgcaaaat ctggtgatca aaaaggaatc atcaatctgc
    19801 acttctttat gtggtttgtc cactatgcgc tttatgctct tattccattc tttgccgttt
    19861 tatttggagc agatgttgtc aaaagcgtct tggataatat tccagaggtc atcatgaacg
    19921 gattaacagt cgctggaaat ctgctgccgg cagttgggat ggcgatgctg ttacggatgc
    19981 tttggagtaa aaatattgct gtgtggtttt tctttggggc cgttgttatg gcgtatctcg
    20041 agttaccatt gattgcaatt gccgcagttg gaacgattat tgcaattgtt atagcccaaa
    20101 gagatctaca actaaaaaat atatctgttc atgcagttca aagcgatgct gctgtaaatg
    20161 caacgcttga ccaagaagag gaggatttct ttcaatgaaa ttagctgatg gaatttctaa
    20221 agatgagaaa aaattggttc ggaagatgtt ttggcgctcg atgactatgt atgcttcggt
    20281 gaatccggtg acgatgggcg gcggtggttt cgcctattct atgatgccct tcattaatca
    20341 tttctacaaa agtgaagaag atcgagctgc ggcgctggaa cgtcatacac agtatttttc
    20401 gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg gcatcgatgg agaaggaaaa
    20461 ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg atcaaaacga gtttgatggg
    20521 accattggct ggaattggcg attcattgtt ctggggagtt tggcgtgtcg ttaccgcggg
    20581 actagcgatc aacttagcca atgccggaaa tattttggca ccaattatct ttttagttat
    20641 gttcaatata cctaattact tgatgcgata ctatggcggc gttttaggct attcattagg
    20701 ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt gaaattctga caaaagctgc
    20761 tagtatcttg ggtctgatca tggtaggtgc aatgacgatc agtatggtca aattcaatac
    20821 cgttttgagc ttttcaatga agggcgaaga actaatgaat gtccaagaaa tcctcgatag
    20881 tatttttaaa ggaattgtac cgttattggt aacattgagc tgtttcaaat tactagataa
    20941 aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc ttgggaattt tattgtcatt
    21001 acttggaatc gcatagtagg aagctgcagc tactaggctg cagttttttt agcaaggagg
    21061 gaaaagatga ctattaatga agtaatttat tcacccaaac acccattttt taatgctgag
    21121 ttaaacgacg gaaatgtgac tacttcaaac gaatacagct actatcggaa tattcctgat
    21181 gtaaagggat tgaccttcga tgtaagtaat caaaagacat tagcaaacct tgatgtttgc
    21241 gggacgattc aaacgttgac cttttatcga gaaaatttac taactgagga aaaaccaggt
    21301 gtttgggtca ataaacagct ctctcaaacc aattcgttat ccttaaaggt tgaagtaaac
    21361 gggattgtct accatttgtc agaatcggat catcgtattg aagtcgatgt gattcaagac
    21421 tgtttgccta gatatattca tcattattcg acgtttaagg tcattgtagt atcctttgct
    21481 cccatactgg ctaataagcg tttaagtatg cttgttcaac aggtttacat tgtgaatgaa
    21541 acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt cgtatcaaga gaagtattcg
    21601 gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg acacgatcgc acaacaggaa
    21661 gtggcaagct tcgccattgc ttttgtggac ccaaatgctt ttgaagaagt aatgactttt
    21721 caggaatctg atatcgaaaa atggctgaag gaaacattga actattttgc tcagctattt
    21781 gggcagctgt caatgccaaa cgatagagtt gtccatctct atcgtcgtgc actttatcaa
    21841 agcttttcta gtttcggtat gaatcgacag ggacaggtcg tcggcagcaa ttggggcagc
    21901 tatcctgcta ctaatcgaat ttggaataag gatatgtatt acgcatcatt gccattcacc
    21961 atgtttgaac cggagttatg tcaaaaaacg gttctatggt ttgatcaata tggcgttaag
    22021 tttcctggtt caaaatttac aggaggtatc aatcattctc tgagtaattc gttatccagt
    22081 gcgttactgg ctagtcttta ttttgaacat actggggata caaaattctt tgagcggtgt
    22141 ccgcaggttc tactgaatgc ttcaagaatt attgaggaca tattagctca acgtcggtca
    22201 ggggaaccaa tgctttttcc atctgtttgg ttatcagacg cctttgcact tggaaagtat
    22261 cataccggca gcaatctttg tctttggaag gcctgtattg gacttgcaga aatttttgaa
    22321 gccctagaac aactgagtct tagcaagcga tacaaaaaca ttgcctgtaa actaaaggaa
    22381 tcaatttgta aacaaatgac tatcgatggt ccttttggtg agcaattttt ggagggaatc
    22441 ggtgatgagg agaaaacaac ctattcagtg aagcactatc aaaaaccgat tcttgagcaa
    22501 gggttaatct ttttaagtga tgtgattgtg gatggaaaaa ttaatttatt gatgcatgat
    22561 ggagaagaat cagacactac gttgatgcct ttttatcaat ttttagataa taaagatcat
    22621 ttatatcaga acaccatggc cttttctgct agttctttca atccaaccta tagcgaggaa
    22681 atcaaaggca tcacttgggg attagaatcc ggcgccactt ttcctggctt tatcactgtt
    22741 ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc gattagaaga actaatttgt
    22801 ttggcagatt tagacggttc gtggtggtgg tggccgtatc gcttagaggc tcaacaagga
    22861 gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg catcgggtct ttttgtcagc
    22921 ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag gagtattgca aattgatcct
    22981 gttgataatt taacgttttc ttggaagaat ttgaagttcg gtcaagcctt catttcgatt
    23041 gaatgtacgc aatccgagtt gagtatttca aatttgtcag aaaagccatt gaaaataagt
    23101 gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga ttcacattca aaggagaaaa
    23161 agatgagtat actaaaaaat attacccaat ttaaaaaaat ccagagtcga gcaatcactc
    23221 ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt gccaagcgag ctaggaccaa
    23281 gccgcaaagg tcgtggcgca attccgctga cttctggtga agaaactgtg attgcggata
    23341 ttactggaac gggaataatt cgtcatatgt ggatgacgat tcgtgatcga acagaatttg
    23401 gcagttttgt tttacgtgat ttaatcttaa gaatttattg ggatgggtcg gatagccctg
    23461 ctgttgaaag tcctttaggg gatttctttt gtaatgggtt tggagagcgt tgcgaggtga
    23521 actcgttgcc gatagtggtt aatcctacag ggggctttaa ttcctatttc gaaatgcctt
    23581 tccaaacatc cgctaaaatt acgatcgaga atttacatcc gaaggatatg tcctcatttt
    23641 tttatacgat aaattacgct ttggtggacg aattggaaga aaatagccta tattttcacg
    23701 caaattggca tcgggaacgc attaccaaat tacaggagga ttataagtta attgatcgat
    23761 taaagggtca aggctattac gtgggtactt accttgcgct tacctgccta gaacgttatt
    23821 ggtggggcga aggcgagttt aaattctaca ttgatgggga tagtgagtat ccgaccgtaa
    23881 cctcaacagg ttcagaagat tattttggcg gggcgtgggc ctttcatgag tacgatgagt
    23941 tcggacgtcc acatgcgaaa aactattcaa cagccttttt agggtatcct taccaatcaa
    24001 atagagatgc cacaagagat cgttttgaaa cgggaaaatt gaatgccgtt catgcctttg
    24061 gtgacgacgc aatgccacga catggtcttt atcgttggca tcttcaagac cccatctgtt
    24121 ttaccgaaga tttatcggtc actttacaac aaatcggcaa cgatgatgtt cgcttatttg
    24181 agcggcaaga cgatgttgct agtgtcgctt actggtattc aacagaagct tcaggaaata
    24241 ctaaacgtca tttcactgat cgagaacgct tgccgcgtta atgaagtgtt tttcacatat
    24301 tcgaagggag gaacctatga tgatatatgg ggcaattgaa gctggaggga ctaaatttgt
    24361 ctgtgcagtc ggtactgagg aattaacgat tttggaaaga gtcagttttc ccacaacaac
    24421 tccagttgaa acaatgaagc aggtcattga tttttttaaa ccttttaagg aacaattggc
    24481 aggcatcggg gtcggctgct ttggtccaat tgatattcaa tgcaaatcaa cgagtttcgg
    24541 tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc aattttgtag gaacgctaaa
    24601 gcaatatttc aatattccga ttgaatggac aacagatgtc aatgcggctt gctatggtga
    24661 atatgttgct ggttctggca aagggttagc aggtgttgca tacttcactg tgggaacagg
    24721 tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa gggttcagtc atccggaaat
    24781 ggggcatatg ttgatcaagc gacatccaaa ggacaatttt tcaggaaact gtcccttcca
    24841 ccaagattgt ttagagggga tggcagcagg accagcgatt gaaaaacggt tgggtgtaaa
    24901 aggccagaat ttattggctg acgattcgtt ttggcagatt gaagcctttt accttgccca
    24961 atgtgcatac aatacaacac tgatgttttc gccagataga attatttttg gcggtggtgt
    25021 aatgaaacaa gaacacatga agaaaaaagt tcaggataaa tttgttgagc ttataaatgg
    25081 atatgttgaa ataccaccaa ttgatagcta cattattacg cccgaattag gtgataatgc
    25141 cggaataatc ggcggattag cattggccag aaaagcggta agaaataagc aaccataatt
    25201 aatgaatgtt tcattgatac tcatgtcaaa agtatttaac cgagtaatca aatgatcaaa
    25261 tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc tctaagtcaa atcgtgttgt
    25321 tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct tttttctttt tgttgtgcgc
    25381 tgtatacctt gaggatattc actggtggcg tacacttaaa tagtaatata ttgtgttact
    25441 ttatgtctgt ggtaatagtt atagttgctt accaatgtat gttcattttt cgaaatgttg
    25501 acttcataat ccttggctta atgtttgaat ttttttggtg atagattttt tcagccgtat
    25561 aaaataaatc gtaattctat tatagagagt tgtgattata aaaagatagc aagaaaaaaa
    25621 aagaaagttt ctatacattt taaatatttt attcctattt ctattgctaa aaaagtagaa
    25681 attctatatt tacctttaac tctcgcttct cttgcaaatg tagttagcat ttt
//
LOCUS       DAZKMK010000028        25358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000028 DAZKMK010000000
VERSION     DAZKMK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.028"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG8V1_001205"
     CDS             complement(132..509)
                     /locus_tag="ACG8V1_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329306.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG8V1_001206"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG8V1_001206"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0329307.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG8V1_001207"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG8V1_001207"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0329308.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8V1_001208"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8V1_001208"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0329309.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG8V1_001209"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG8V1_001209"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0329310.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG8V1_001210"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG8V1_001210"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0329311.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG8V1_001211"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG8V1_001211"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0329312.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG8V1_001212"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG8V1_001212"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0329313.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG8V1_001213"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG8V1_001213"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0329314.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG8V1_001214"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG8V1_001214"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0329315.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG8V1_001215"
     CDS             10661..11575
                     /locus_tag="ACG8V1_001215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0329316.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG8V1_001216"
     CDS             complement(11708..13273)
                     /locus_tag="ACG8V1_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329317.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG8V1_001217"
     CDS             complement(13266..14717)
                     /locus_tag="ACG8V1_001217"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0329318.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG8V1_001218"
     CDS             complement(14731..15747)
                     /locus_tag="ACG8V1_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0329319.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8V1_001219"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8V1_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0329320.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8V1_001220"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8V1_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0329321.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8V1_001221"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8V1_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0329322.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8V1_001222"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8V1_001222"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0329323.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG8V1_001223"
     CDS             21340..22764
                     /locus_tag="ACG8V1_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0329324.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG8V1_001224"
     CDS             complement(22806..23063)
                     /locus_tag="ACG8V1_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329325.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8V1_001225"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8V1_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0329326.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG8V1_001226"
     CDS             24337..25314
                     /locus_tag="ACG8V1_001226"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0329327.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8202 a         4317 c         5695 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAZKMK010000029        24947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000029 DAZKMK010000000
VERSION     DAZKMK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.029"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8V1_001227"
     CDS             <1..1152
                     /locus_tag="ACG8V1_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0329328.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8V1_001228"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8V1_001228"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0329329.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8V1_001229"
     CDS             2728..3003
                     /locus_tag="ACG8V1_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0329330.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8V1_001230"
     CDS             3009..3776
                     /locus_tag="ACG8V1_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0329331.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8V1_001231"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8V1_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0329332.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8V1_001232"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8V1_001232"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0329333.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8V1_001233"
     CDS             7920..8222
                     /locus_tag="ACG8V1_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0329334.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8V1_001234"
     CDS             8225..9388
                     /locus_tag="ACG8V1_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0329335.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8V1_001235"
     CDS             9414..12842
                     /locus_tag="ACG8V1_001235"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0329336.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8V1_001236"
     CDS             12863..13987
                     /locus_tag="ACG8V1_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0329337.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8V1_001237"
     CDS             13968..14414
                     /locus_tag="ACG8V1_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0329338.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8V1_001238"
     CDS             14398..14736
                     /locus_tag="ACG8V1_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0329339.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8V1_001239"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8V1_001239"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0329340.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8V1_001240"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8V1_001240"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0329341.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8V1_001241"
     CDS             15893..16936
                     /locus_tag="ACG8V1_001241"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0329342.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG8V1_001242"
     CDS             17095..17823
                     /locus_tag="ACG8V1_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0329343.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG8V1_001243"
     CDS             17939..18184
                     /locus_tag="ACG8V1_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0329344.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG8V1_001244"
     CDS             18181..18399
                     /locus_tag="ACG8V1_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0329345.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG8V1_001245"
     CDS             18535..19074
                     /locus_tag="ACG8V1_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0329346.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG8V1_001246"
     CDS             19067..21340
                     /locus_tag="ACG8V1_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0329347.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG8V1_001247"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG8V1_001247"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0329348.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8V1_001248"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8V1_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0329349.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG8V1_001249"
     CDS             complement(23167..23436)
                     /locus_tag="ACG8V1_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329350.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG8V1_001250"
     CDS             complement(23698..23826)
                     /locus_tag="ACG8V1_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329351.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG8V1_001251"
     CDS             complement(23906..24361)
                     /locus_tag="ACG8V1_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0329352.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..>24947)
                     /locus_tag="ACG8V1_001252"
     CDS             complement(24499..>24947)
                     /locus_tag="ACG8V1_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFQ0329353.1"
                     /translation="KTQLVKKENELYQVNAMVGDGVNDAPALAAASLGVAMGQGTDAA
                     MDIADIVLMKSDLNKFVYSHRLSLKMRRIIKQNIIFSILVILLLIASNFLQFLNLPLG
                     VVGHEGSTILVILNGLRLLRPLPDLSDSSKKCKSCPLYKARLSSVK"
BASE COUNT         8354 a         4302 c         5311 g         6980 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gtttttt
//
LOCUS       DAZKMK010000030        24377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000030 DAZKMK010000000
VERSION     DAZKMK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.030"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..635)
                     /locus_tag="ACG8V1_001253"
     CDS             complement(<1..635)
                     /locus_tag="ACG8V1_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0329354.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSK"
     gene            complement(1203..2270)
                     /locus_tag="ACG8V1_001254"
     CDS             complement(1203..2270)
                     /locus_tag="ACG8V1_001254"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0329355.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2290..2769)
                     /gene="pssE"
                     /locus_tag="ACG8V1_001255"
     CDS             complement(2290..2769)
                     /gene="pssE"
                     /locus_tag="ACG8V1_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0329356.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2786..3247)
                     /gene="pssD"
                     /locus_tag="ACG8V1_001256"
     CDS             complement(2786..3247)
                     /gene="pssD"
                     /locus_tag="ACG8V1_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0329357.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3261..4511)
                     /locus_tag="ACG8V1_001257"
     CDS             complement(3261..4511)
                     /locus_tag="ACG8V1_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0329358.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(4889..6340)
                     /locus_tag="ACG8V1_001258"
     CDS             complement(4889..6340)
                     /locus_tag="ACG8V1_001258"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0329359.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(6661..7425)
                     /locus_tag="ACG8V1_001259"
     CDS             complement(6661..7425)
                     /locus_tag="ACG8V1_001259"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0329360.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7453..8151)
                     /locus_tag="ACG8V1_001260"
     CDS             complement(7453..8151)
                     /locus_tag="ACG8V1_001260"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0329361.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(8163..8942)
                     /locus_tag="ACG8V1_001261"
     CDS             complement(8163..8942)
                     /locus_tag="ACG8V1_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0329362.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(8958..9902)
                     /locus_tag="ACG8V1_001262"
     CDS             complement(8958..9902)
                     /locus_tag="ACG8V1_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0329363.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(9948..10727)
                     /locus_tag="ACG8V1_001263"
     CDS             complement(9948..10727)
                     /locus_tag="ACG8V1_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329364.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(11107..13182)
                     /gene="glyS"
                     /locus_tag="ACG8V1_001264"
     CDS             complement(11107..13182)
                     /gene="glyS"
                     /locus_tag="ACG8V1_001264"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0329365.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(13184..14101)
                     /gene="glyQ"
                     /locus_tag="ACG8V1_001265"
     CDS             complement(13184..14101)
                     /gene="glyQ"
                     /locus_tag="ACG8V1_001265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0329366.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(14487..15305)
                     /gene="recO"
                     /locus_tag="ACG8V1_001266"
     CDS             complement(14487..15305)
                     /gene="recO"
                     /locus_tag="ACG8V1_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0329367.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(15448..16347)
                     /gene="era"
                     /locus_tag="ACG8V1_001267"
     CDS             complement(15448..16347)
                     /gene="era"
                     /locus_tag="ACG8V1_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0329368.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(16362..16757)
                     /locus_tag="ACG8V1_001268"
     CDS             complement(16362..16757)
                     /locus_tag="ACG8V1_001268"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0329369.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(16741..17217)
                     /gene="ybeY"
                     /locus_tag="ACG8V1_001269"
     CDS             complement(16741..17217)
                     /gene="ybeY"
                     /locus_tag="ACG8V1_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0329370.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(17246..19438)
                     /locus_tag="ACG8V1_001270"
     CDS             complement(17246..19438)
                     /locus_tag="ACG8V1_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0329371.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(19462..20433)
                     /locus_tag="ACG8V1_001271"
     CDS             complement(19462..20433)
                     /locus_tag="ACG8V1_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0329372.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            21138..22517
                     /gene="brnQ"
                     /locus_tag="ACG8V1_001272"
     CDS             21138..22517
                     /gene="brnQ"
                     /locus_tag="ACG8V1_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0329373.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            22799..24139
                     /locus_tag="ACG8V1_001273"
     CDS             22799..24139
                     /locus_tag="ACG8V1_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0329374.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         6942 a         5121 c         4075 g         8239 t
ORIGIN      
        1 atttttgaat gttgcaaata tgaaatatgg aatatgatat cttcagatat ataatccctt
       61 tctgacggaa acttgatatt gtgctgttga attatctcag atgaatacag cgcatgccaa
      121 actgacattg gcaatataaa atcgacttta taactcggct cagtgccaat cattccaagc
      181 aacacttttt caattatagc gttatttgta aatatcgtac tatcatatct tgctggcttt
      241 gaaataatat tattatcatc ataatattca taatatccag cataacatgt gtctgcatta
      301 ctccttttta taccatttaa cattgcttca atataattag ctgcaacaaa atcatcagaa
      361 tcaataaaag caacatattt tccttttaca tgttcaagtc ctgtatttct tgccaatccc
      421 aagccagcat tatttttatg aataatacga atccttgaat cctttaatgc atagtcttca
      481 catattttat tagatgaatc cttagatcca tcatccacaa ttataatatc taggttctta
      541 tatgtttgat tacacactga ttccaaacaa cttcctacat acttttcaac attatataca
      601 ggtatgatta cactaattag atcactcgaa ttcatccttc tatcctttca ctcacgtttt
      661 ctcaattagt caatgacggc atcaacgatt ttcttaaatt ctctgaaaaa aatagactct
      721 gatcttgatg atgtgaatga catactatct tcttctagaa aaattatttc ctttaaatta
      781 tttattaaat caataatatg atcctcgtaa gtatgctttc ccttggaata atgaaaatat
      841 acttttgtat tacacaatat tgcaatttta tttcttaata tgattatttc actcattcat
      901 tactgccaca tatacacaac ttataaccac cccctgcaaa gtttagggta agttatcaga
      961 taatatcttc ctcttcatca tccagaatac aaaatctatt tatttataga aatatcatta
     1021 atgtttttat ataatgtacc gtgcttgtac tcttgctcta ttacaagcac taaaacacta
     1081 ctattcattt gaattcttac acggataaaa atttacatcg tctatttcaa tattttttct
     1141 ctctatttta ttcacttaat aagttcatta cataatcgaa acatttttta ttttccttgt
     1201 catcactcct tgctcccttt ttgatacaga tttaaaattc tatcaaagaa atttccatcg
     1261 ttaaatttcg ttattgccaa atccttcgaa caacgtgaaa attttacgta tttatcttta
     1321 tcattgattt ttagtattgc agatgtcaac gctgatacgt caccaggttg aaacaaatat
     1381 ccattttctc catcacgtac tgctgaagaa atatctccta catctgtagc aattacaggc
     1441 attccataag aaatcgcctc tagaatagat ataggcaatc cctcgttata ggacggtaaa
     1501 atcatcacat ttgatcttct atagcactcc tccttttttt ctcccacaac ccaacctgta
     1561 aattcaaaat acgattgcaa attatattct tctatcaatt ctttgagcga tatttcttca
     1621 tcacctgctc cagcaatctc aaaaataaac tttttttctg gatattgttc tgcaatgttt
     1681 ttaactgctt ctaccaaatc gtgtacacct tttcgcttta tcaaaactcc cataaacaga
     1741 aatcggcaca tttttgaatt ccactttatg gtatattctg gaactacaat agaattttct
     1801 acaacaacag ttttacaatt tggctcaatt tctttaatta tcttcttcca tttttctcca
     1861 agtacaatcg tggaatcaga ctctcttaac agcaagcgaa tctgattttg tttttttaca
     1921 gaaacactat cataccattt tttaaattcc gatccatgaa gatggatgat tacaggaata
     1981 tttaataatc taaaccactt attcagcaca tatttcctac taaaactccc tctgtacgac
     2041 atgtgcatat gaataatatt aggtttactc aataaagaga tacctataat tttagcaaat
     2101 gcatacataa aatataaaga tttaaatatt gcatttccac ccttataact cggtataaaa
     2161 gtcatctcga tattcttttt ttcccaatta aaattgagca tttgtgtaat tatacttgta
     2221 attccacctt ttaccgagct gtcattacca atcataagta ctctttgcat aacgctttac
     2281 tccttatatt tatatttttt ctattaatac ctctatcgaa ttaataatcg tttccgtgtt
     2341 agatacatac ttgttaaaca tccgaactct tgtttcttta acggcatctt ctaaattttc
     2401 taattcataa caaggataga taatattcat ctcatcaaat tgtctaatta actgcagttg
     2461 gtgatcatcg acatgctcac catacttagc caatcgagga acagcaatga cccttttacc
     2521 tttttttacg gccccaatta tagcacctgt acctccatga gtaatcacca tttctgcttt
     2581 ttgcatagtt tcagtaaact catccctatt tagaaatgct ttataggaat aatttagtgg
     2641 tttataatca ctataaccac tctgtgcaaa tgtactttcg attattactc ctttttcaat
     2701 taaatcatca atagctttta acaatctatc aaattgaaat ttttgtgaac ctaatgttat
     2761 aaatatcatt tttttctcct attatttaat atatcccacc caaataaata gcattaggat
     2821 aaaattgtag catagtttcc cactgaacat aaaattgatc tgcaaaatga tacatcaatt
     2881 ttcctgtctg agtagggctg gttacttttg caaacgattc aatataaatc agtttttttc
     2941 ccatcatttt tgttattaag cagataggga tcatagctaa aacacctgta caaatcacga
     3001 gatccggttt ttcctttata tatataataa ttgatttgaa taaattaata agtatagtac
     3061 acaaaaaaaa cttttctttt ctatttactt gtctcatgta gtacgttttt tcgccttttg
     3121 actcagctga atattctgtc tgttctgtta taacaaaact ctcatacttc tccatcagtg
     3181 gttttaacat cattaactgt tcataatgcc ccccagagga agccgcaaaa catatctttt
     3241 tgttcatatt aacctctcta tcagtcacgc tgaaaaatat ctctagtgta aactttttct
     3301 ttaatatcat ccaagcaggt atcatatcga ttggcaataa tagaatcaca catcgcctta
     3361 aactgctcta aatcgttgat aaccttactt ccgaaaaaag tctctccatc tttcagtgca
     3421 ggctcataaa tcaccacagt cgcaccttta gcttttacgc gcttcataac tccctggatt
     3481 gaactctgac gaaaattatc actgttgctc ttcatggtca gccggtatat accgattaaa
     3541 actggatttt ccctgttgac atcatactga ttatattcag cgtagttgta atagcccgcc
     3601 atctgaagaa cacgatctgc gatgaaatcc ttacgggtac gattagattc aacgatagcc
     3661 tcaatcaaat ttccaggaac atctgcatag tttgccaaca actgcttggt atcttttggc
     3721 aagcaataac ctccatatcc aaaggatgga ttgttgtagt gagttccaat acgaggatcg
     3781 agacaaacac cattgattat ctgctgagta ttcagcccct tcatttcagc ataagtgtct
     3841 aattcattga aatagctgac tcgcagtgct aaataggtat ttgcaaacag ctttactgct
     3901 tcggcttcag tgaatcccat aaaaagagta tccacgttct ccttaattgc acttttctgc
     3961 agaagtgttg caaaaacatg agcagcattt aacaagcgct catcagtcat atcagtacca
     4021 acaataatac gagatggata cagattatca tataaagcct tgctctcgcg cagaaactct
     4081 ggactaaaaa taatgttctt actccccgtt gtctcacgga ctgattcagt gtagccaaca
     4141 ggaattgtag acttgatgac catgatggta tcaggattca cgtccatcac aacctcaata
     4201 actgactcaa cagcagaagt atcgaaatgc tgagtacgac tgtcatagtt agtcggtgta
     4261 gcaataacaa cgaaatctgc ggatttataa gcgctccttg catccaaagt agcagttaag
     4321 tcaagttctt tttctgtaag atacttttca atatattcat cctgaattgg agacttccgt
     4381 ttattaatca gttcaatctt ttcagggata atgtctaccg cggttacctg gtgatgctgc
     4441 gccaatagtg tggcgatact caaaccaaca taaccggtgc cggctactgc gatgttgtac
     4501 tttttcttca caatatctcc tctttcctct cttaagtatg gagaatgtta ctcctacggc
     4561 tattcatcat gtcaaaataa cacagcttag aatgttgata aaataatttt ccagaatgtg
     4621 ataaaatctc tttcatttat aaaccacaca tctaatttta tctattctcc acaagacatg
     4681 tagagtattc tttaataaca agatacgacc ttactaaata cttcaaaaaa ttatcatatt
     4741 taattcataa atataaatct tcattttaaa tgtatgccca aaaatttatc tgtaatgcgt
     4801 catcaaaatc tagattccaa atctttatca atttttatcc actcattcta ccactttagt
     4861 aatattttct atttataaca ctttcgtcct acattgatcc ccccctctga agtacagttt
     4921 ttactgtctt aaataaaatt ttaaaatcta accccatact ccaatttttt atatattccg
     4981 tgtcaagttt tgctacttct tcaaaatcag taatatcact tctaccactt acctgccaca
     5041 ttccagtaat tcctggctta atagcaattc ttgcacgatg atgataatca tattcttcat
     5101 actctgattt aaggcatggt cgaaatccta taaggctcat ctttcctgtt agaacgttga
     5161 aaaattgagg gaattcatct aaagacgtgg atctaataaa ttgaccgatt cctgtctttt
     5221 ttgttccatc tggaagtatt ttatttccga taattcttgg atcaaactcc atcttaaaca
     5281 taagattatt gctcatctta ttttctttca ttaattctac ttttctttct tctgcatcca
     5341 tatacatact tctaaactta tacatcttga acggtttacc attctgacct atacgttctt
     5401 gtgcaaagaa aattgggcct ggtgaattaa tataaataag tggagcaata aagataaaaa
     5461 taattcccgt tgcaatacat ccaacaatac ctgcaagaat atcaattgtt cgttttataa
     5521 atgcctgttg agttgtcata acattgatac ttgttgtaag tactgtatat tctcctatac
     5581 gaccaataga ttgttttccc aaagcactat ctgatacttt tgcaagattc aaatgtacag
     5641 taaggcccat ttcagagcat cgttcaataa attctttcat acacgaagta cttttattca
     5701 aaataataaa gacttcatct acccatccct gacataagta atccgctgca tttttggaat
     5761 cagaaacaac tgtcactccc tcaacatctt caccaatcat gcttttatct atgataatga
     5821 caccatttat tttatacatt ttatagttat gctctttcac attacgaata actacttcag
     5881 caatactact agatgtaact ataataagtg atttgttccc ctcatttttc attttatgtt
     5941 tcaagtaatg tttccataag aggcgagtta tatatgtaat tacaacgtat aaacctccca
     6001 tcaagaaaag aacgactcta gaataatcat ttccaccctg aacactaatg agatacaaac
     6061 ttcctaacag caccaacata atcgcttgct ttacagaaga agcaaattct atatagtacc
     6121 ctctttttaa tacacctcta tatgcttcaa acatgaaaat aactaaaaga tctgccagtt
     6181 caatgaagac tgccatgttt tgatacattg gaatcacata aggattccaa tctacctggc
     6241 gtaagacata cgatagataa aaagcaagct ggatacacaa taaatctaaa ataataaaat
     6301 caatatgttt aacccatccc cttgaacttt tcttgtacat tcctttttac tcccctcttt
     6361 ttaataaaat aaattgtatt acttgttttc tctcctaaat atctttctga aggattattg
     6421 actcgacaaa ttggcttgtg gatattctta tatatagaac atccgtaagc caacttaaaa
     6481 ctccttaaca aaattttttt actcatattg tagatcaaaa caagtatcca agaaatcata
     6541 ttttatttct tttttctgct tactcaacaa cagctaaaag aaaaatcatc ataaacaaat
     6601 tcaactaagc caacgatccc cttgtgaaat caagtttttt cttgatttac gaataaagag
     6661 ctaaaataaa ccaaacttct tcttttttac ctcttcatag actggataac tgatttcatc
     6721 cccgttgatc agatcctttg cgacttgttt catctgtact actttttcct taccaaagtc
     6781 ctttgcaatc tgctcatagc actcttttaa gtaaaaggtg cgcttagaga ccccatgggc
     6841 atcagaagca gccatctgca cgagattatg tttgaccatc gttttggctg ttttctggat
     6901 gtctttgcca aataccccaa cataacttgg cgcagtgagt tgggccaaac atcccatatc
     6961 taggaacgga agcaaatgat ttgggtcttc gcgaaaatgc acgttgcgtt ctggatggac
     7021 gattactggt gtctttccta gttctaacaa ctcaaaaaaa agccgttctg catataacgg
     7081 cacttccagt gtcggaaatt cgatcagaag atatgtatca tctaaatctg taaataatat
     7141 ttcatctcgg tggatgtctt cgatgagcgt tccggtaata cgtacttctt gtccttccaa
     7201 taacgtcaaa ggcaactgtc ttttctctaa ttcggcttgt aacgaagcga ctagagaaat
     7261 gacttgtgat ttttcattgc tgtattttcc attattgtgg tgtggtgtgc ataaaatatg
     7321 cgtgatgcct tgctgaatcg ctttttcagc cattgcaata cttgcttcta agttttctgc
     7381 cccgtcatcg attcctggta ggatatggca atgtagatcg atcatttctc ttgtctcctc
     7441 tcttcttatt ccttaatttc cgtagtaata accagcatct ttgctatgtt ctgctccgtt
     7501 gtacacaaca cctaagactc gtgcttgtac catctccagc agttcttttg ctttagtcaa
     7561 ggattcttta cgtgacatat tttcacgtac gaccaataaa gtgccatccg tttttaaaga
     7621 catgatctgt gcatctgtta cggctactac tggtggcata tcgaatatca ccacgtcaaa
     7681 taactgtctt gcttcggata ggatctgatc cattcttggc gagcttaaca attctgatgg
     7741 gttgggtggc ttaggtccac taggcaaaat cgacaagttc tcgactggcg ttcgttggat
     7801 cacatctgct acacttcctg atgaactaag tgctgtactt aatccactgg cattgttcag
     7861 ctggaatgtc ttatagacga cgggtttacg taaatctgca tcgactagta gaactcgttg
     7921 acctgattta gcaaaaacga ctgcaatgtt tgctgcagtg gtggatttcc cttctccagg
     7981 acctgatgaa gtcacgacaa tcgttttgat ttgctgacca gctgcggagg caaactggat
     8041 attcgtccgg atcgtacgat attgttcggc gattggtgaa gatgggtttg ttaatgtgat
     8101 caaactgact gcatgagttt gtgctgcttt ttgtttctgt gttcgtgcca tttttcttcc
     8161 tcctagactc ttgagcggct tctgcgtgat ggggctgttt tatcgtttgt agcttttggt
     8221 cgtgcaggag ctgttccttt tttacggata ccagcgttca attctttcgc tgacatttct
     8281 ggaactgttc ctaagattgg gaatcctaat gtatctgtca catatttttc atctttcact
     8341 gtccgatcga gtaattccaa taagaatgcc agacctacac ctaccatcat tcctaacatt
     8401 aagccagtca ttaaattcaa tttattattt ggcgaaacag gtgtcgtatt ggcaactgca
     8461 cctgaaatga tcgagatttt atcaacattc atcacatctt ttgcgttctc ttggaaaact
     8521 tgtgaagttg tgttagcgat ctgttgagca gccactgcat tcgtacttat tgcctgaatc
     8581 gagaacattt gtgaattttc tgcctgatta actgagatgg catctttgat ttgttcagca
     8641 gtcattttca cattgtcttc tgtttctaaa cgatctttga cttcattgag cactaaatca
     8701 ctgacgatca tatctttata cgtattgatc atttgcaggt tcgtattgac atcattgaca
     8761 tttgcagtgt ctgattgtgg cagtgtcacg atcaattgtg cttgtgaact gtattttggt
     8821 gtcaagatga aaaatgttac gattcctgag atggccaagc cagcaaacat tgagatgatg
     8881 attattacca agtgtttgcg taatgtttca aaaatttctt tcaagctgat tgtctcttcc
     8941 attttttctc cactttccta tttattctct agattcattt gatcttttaa ttcttttttc
     9001 acacgatcaa gttcttgatc actgactttt tgataggaga tattatcttg catgaagcct
     9061 tctccttgca gctgatcttg cttaatcgta ccaaatgcct cccgataatc taaagcaatt
     9121 tttttcattt catcaaagtt catgtctgtt ttcatattcg actctagagc attcaaaacg
     9181 gattgatagt tgctgacacc gtttagactc aatacttttt tcgctacgcc ttctacgatc
     9241 tttctttggc gcccttgtct accgtaatca ccatttgggt cttcataacg catacgagaa
     9301 taagataatg cctgatcccc gttcaatgta attttaccga ttggaaagtc atagccatct
     9361 tgtgagaaag tgaggtcatt attgacttcg ataccaccaa ctgcatcaac gagttccttt
     9421 agtcctttca tattgataga gacatagtga tcgattggga tgtctacata tttttgtacg
     9481 gtatccatcg ccatggcagc tccgccgaat gcgtaagcgt ggttgatctt gtcttttgtg
     9541 tcacgaccta cgatgtcgac atacgtatct cgaggaatgc tgacgatcgt tgtctgtttg
     9601 tcttcaggac tgactgtggc aaccatcatc gtatctgatc ttccttgatc gacccgacct
     9661 aaatctccag tatcaatccc catcaataga acagagaaag attcttgttt gtccagatca
     9721 actggtttcg ttcgttctcc ttgggttcgt tccactgatt cgtaagtttt ctcaatagaa
     9781 tgtgagagat ccagatataa ttttgctcct actcctacaa ttgctaatac taatacgata
     9841 agaactgcta aaataatcaa aaatattttt ttccctcttg attttcttct tcgtcttgcc
     9901 atagtttcct ccgtttgcta accttgcttg aatcaacgtt ttaactgtca atagcttata
     9961 ttgctatagc ccatttgctg agcaacagtt tgtgcttttg ttgcgtatgc ggctcttatt
    10021 ttttcattcc ggatctgctc tatttgactg agcgccgtca aatattgatc gtaagtagcg
    10081 gtagctgtta cttgaccgtc tttcagcatc gtatcgaata gctgatctag ttgcgtgact
    10141 tgtgcaattt gggtatcagc gaatcctaga tattgcataa cgactgtttt ccaagcacta
    10201 tccttaaaaa agctcatatt ttcacgtaca tgggcgacat ctgcacttgc caacttttct
    10261 ttgatgatga catcatccac agcttgctgc cagtttgata cgtttttagt aaataattta
    10321 ttgacagccg tctggatctt cagttttgat tctgcatcct ctaactgcgt taagactgct
    10381 ttcttttctt cttggatcgc agcagcttct ttaggtaatt ctgattcctt gatgtcataa
    10441 tcatctgccg aaagtttgat agaagatagt ttatgtgaca aatttgaaac tgtttcttct
    10501 gttatttcag gatttaggaa aattttttct tttgtactgt acagcttatc tacttctttt
    10561 gataaagagg tcacggtgtc tttttgattg gtgatggcag attctgcgta attgatccgt
    10621 tgatgatatt gccgctgatt attgagatgg tatccaaaca agcaaatcac aatagcgcct
    10681 gtgcctaccg ccacagtagc caataacttg acgaacggct tttccataga ttcactccta
    10741 aatattgatg atagataagt aaagatgtga ttcattcaca tcagaatgag cgctttttat
    10801 tttaaaagca taattaaact aatatattca tagaaatatt tataaacagg ttttccctat
    10861 ataatcatac cacatacagt ttttttgact agggaaattc ctaatttaaa agtaaataaa
    10921 acagtaacaa gattataatt aatcgttttc atttttattt cctcactttt catgagcgtt
    10981 ttgaaaattc gaacaaagaa taaaataaca aaaattatta aaatagaatt aataacagct
    11041 atttataaca aaaaaaacag agacacagca cgtttcgtgc atgctctctg tttttcaaac
    11101 aaaagactat tttgtgatga tttgatccaa gcttgctaat gacaaagcca tggcagctat
    11161 ttgacttagt tgtgttagtc ggttcttacg tactgctggt tcatcagcca tgaccatcgt
    11221 ttgatcaaaa taattttcga tcagtggacg aagagcgaca agctggtcgt agttttcgtg
    11281 gatcgtttgg tcagcaaaac ttgctttcat tgcttctact gctttgtgta attctttctc
    11341 tgcgtcgttt tcgaacagct cagggtctac ttctgattgt ttttcaagct gcgctttttt
    11401 cgctaagttc acgacacgtg tcaaagcttc cattgaaggt ttgaagtctt catctgcaag
    11461 atgttgtttg aacaattgtg ctgctgcaaa tacttttgtc agatcttttt gttccgcatt
    11521 caaaacagca tcgatgacat catgacgaat attcttcgtt gtcaataatt ggcgcagacg
    11581 gcctttgata aagtcgatca cttgttgttg cccttctgat aaaccaatgc cgaaacgatc
    11641 cgtatcttta ctgataacat cgctaatcgt ttcttgcaga acagacaaag gaaaggccca
    11701 ttttttcgat tcgatgatcc gaacgacacc gtatgcttgg cgacgtaagg cataaggatc
    11761 gtttgacccg cttggaatca tcccaactgc aaagaaagaa aagatgctat ctaatttatc
    11821 tgcaatcgct aaaacagaac ctactgtaga ctgaggtagc tctccttcac tggatatagg
    11881 catgtaatgc tcacgaatcg ctgtcgctac agcaggtttc tcgcctttca gaagagcata
    11941 tttttcgccc atgatccctt gtagttcagg aaattcccct accatgttgg ttactagatc
    12001 aaatttatag atttcagcag ctcgttgcaa atccatcaat tcttcctcag atagtcctac
    12061 ttgttcgcca atgatacggg caatgacttg tacccgttgc attttctcat agatactacc
    12121 gattttttca tggaaagtaa cagtttttag tttttcaacg tattggtcga ttgaagcttt
    12181 cttgtcttca ttataaaaga attccgcatc ttctaaacga gccgtcaata ctttttgatt
    12241 gcctcgaacc acatcttcca attttacccg atcaccatta cgagcagaaa taaaatgagg
    12301 aagcagcata ccttgatcat tccgtacctc gaaataacgt tgatgttctt tcatagaagt
    12361 gactagtact tcttcaggaa cagaaagata tttttcttcg aagtcgccag aaaagacagt
    12421 tggatattcg actaaattgt tgacttcttc tagtaaatct tcgtctagat caataaccca
    12481 gtgatttttt tctgctaatt cgtttgcttg tgtaacgatc atttcttgtc ttttttgcgg
    12541 agacgcaatg acgaattgtt cattcaattt ttcttcatat tcttttgcat gcgcaaatgt
    12601 cacatcttcg cctaagaaac gatgacctcg agaggctctg cctgttttga catccaagat
    12661 ttcgaatggg attacttcgt catctaacaa tgccacgatc cagtgaatag ggcggatata
    12721 ttcaaaatcg tattctgccc aatgcatcgt tacagggaaa gtcaaacttt tgatcacccc
    12781 tgctaagcct gtcaatactt cttttgcttc cttaccatga gtgtatttcg ttacatagac
    12841 atattccaca ccttttaatt ctctaaaagt gatagcgtct gtcgtcagac cttgtccgcg
    12901 gacaaatcct tccgctgctt tcgtccagtt tccatctgcg tccaaagcaa tttttttggc
    12961 tggtcctttg acttcttctt cactgtccgc ttgtcgttct ggaatatttg ttactttgat
    13021 tgctaaacgg cgcggtgtgg aaaatgtttc gatcgtatca taagtcaatt ggttttcatc
    13081 taaaaatttt gctgtttttt cttctaattg tttcatgctt ggtgtgacaa catgggcagg
    13141 catttcttcc agcccgattt caagtaataa gtcttttgcc atcttattca gcctccttca
    13201 cggtttctgg atttgctgtg tttttattca acaaagggaa tcctaatttt tcgcgttctg
    13261 ccacaaagat tttggctaca gcacgagcca tgttgcggat gcgagaaaga taaccggcac
    13321 gctctgttac ggatactgca ccgcgggcgt ctaataagtt gaatgtatgg ctacatttca
    13381 agatataatc atacgccgga tgaacaagat tttcatcgat acagcgttta gcttcttttt
    13441 caaaacgatc aaagttgctt agtagcaagt cttggtcgct tacttcaaaa gaatatttgg
    13501 aatgttcata ttctggttgt ttgaagatct cgccatattt cacgccatca gcccattcta
    13561 aatcatagac actttcaact tcttggatat aagaagcaag acgttctaat ccataagtga
    13621 tctcagaagt tactggttta caaggcagtc caccaacttg ctggaagtac gtgaattgtg
    13681 tgatttccat tccgtcaagc catacttccc aacccacacc ggcacagccc atggatgggt
    13741 tctcccagtt gtcttcaaca aaacggatgt cgtgttctaa tggatcgatt cctaaaaggc
    13801 gcaagctttc taaataaagc tcttgaatat tctcaggtga aggtttcatc accacttgga
    13861 attgatgatg ctgatacaaa cggtttgggt tttcaccata acgtccatca gctggtcggc
    13921 gagatggctc tacgtaagca gcattccatg gttctggtcc gattgcccgt aaaaaggtat
    13981 atgggctcat cgtacctgct cctttttctg tgtcataagc ttgcatcagc atgcagccgt
    14041 ttgatgacca aaatttttgt aatgttaaaa tcatatcttg gacggtcaat tttttactca
    14101 tctgttttgc ccctttcaat tttaaagcgt tgctgtcaaa taagaataca ggaaagaaaa
    14161 cgacaaaaaa tccctatgtc tgattacaga catagggacg aataattcgc ggttccaccc
    14221 tacttccgaa taaagattcg gcagcttcat ttatcgtact cgcaagtgcc tcttcaaaaa
    14281 tcgtttgggt ccggctccca ctctcccgaa ctcgctaaac cacgtgtttt ttactgatct
    14341 tggtcaatgt acgtattcat tttgcagttt tcttgtttct ataatagatc ctatcactga
    14401 acgatctatt taatctatgg ttaataatag acggaactcc ctgtgtttgt caaatggttt
    14461 tgattttttg ttgtatgttt tatttactat tcttcttttt caggtgtctc tttttctttt
    14521 tcagacatgt ctttctctga cgtctttaat acgtgtgccc aagacttcat ttgattgatg
    14581 aactttttgc ttttcaaatg gataccgaca tattcatcat acagctggtc gatgagttca
    14641 cggattgctt gtttggtttc ttctttcaga tggattccac taattttatc ataagaaata
    14701 gctgaaaaca tccgaacgaa ataaacagct cgaggatctg catgataacg gtgatcgtcc
    14761 atcgaccaat gtctttcgca tatcacacca ccatatttgg aagaatagtc aaattttcct
    14821 tttgtttccc cgcatacgac gcagtgtgtc cagattggtg caattccgaa gcgttgcagt
    14881 atttggactt caaaaatatt cgtgatgatt tctgcatcgt ctccttggtc catccgttgc
    14941 agtgcttgtt gcgtaaactg gaacaaatgc gggtcgtact gatgatcttc gattgccgca
    15001 tctactagat tcaataaata tgtgccatag ccgctcaaaa agatgtcttc ttggatgtgt
    15061 cgaaacgcat ggacatcttt gacactgctt aaaaatgaca aaccttcact tcgaaaattc
    15121 ccgatataaa ctgcttcagt aaaagggagc aaagccggag ctaagggctg attttgccga
    15181 tggatctctt tgatataaaa catcattttg ccagaagatt ctgtaaagat tttgactaat
    15241 ttatcttttt ctttgtaatt acggctagat aaaatcaagc cttttgattc tgcaagctgg
    15301 ctcatattct ccctcctaaa aaataaatct ggaaaagcag attctgccga tccagatcat
    15361 ttatgttgcg atcttccgca gcttttctca acacgcggat ttttattcag tttatttcta
    15421 tacctaattt acaagaagct cagaatatca gtattcgtct tttcggtagc cgaagtcttg
    15481 cagatagact tttttgtctc gccagtcttt ttgaactttg acccaaagtt ctagaaagac
    15541 tttgtctcct aaaagatttt cgatatcttt tcttgctttc gttcctactt gcttcagcat
    15601 ttttccgcct ttgccgataa tgatcccttt ttggctatct ctttcgacaa taatcgtagc
    15661 ctggatatgg attttatcat tctcgtttcg cttcatcgaa tcaacaacga ctgcgacaga
    15721 atgaggcact tcgtctcgtg ttaaaaacaa gactttctca cggatcaatt cagaaacgat
    15781 gaagtattca ggatgatcag tcacttgatc atctggaaaa tattgtgggc cctctggcat
    15841 ttcactgact aatacatcca tcaaacgttc gacgttgttt ccttctgtag cagagattgg
    15901 aatcacttgt gcaaaaggca tctgcgttga ataatcttcg atgattccca acaactcgtc
    15961 tggatggatt ttgtcgattt tattgataat cagataaaca ggagaatgca cgttttttag
    16021 acgttcaata ataaaatcgt ctccccgacc gcgtttttga tcagcactaa tcataaataa
    16081 gaccgcgtcc acttctttca acgcactgta agctgcttct accataaaat ctcctaaacg
    16141 atgctttggt ttatggattc ctggtgtatc aataaaaatc aactgagctt ctggtgtagt
    16201 gtatactcct tggattttat ttctagttgt ctgtgccttg tcactcatga tggcaatttt
    16261 ttgacccacg attcgattca ataatgtgga tttccctaca tttgggcggc cgacgattgc
    16321 gacaaaccct gatttatgtt ctggcatata tgttctcctt tttaattaaa caacaaatga
    16381 tatattttag ggacaaataa aatcaaccca acgatgacgg taaaacaagc ggtcactaaa
    16441 actgctccag ctgccatatc tttgattttt ttcccaatcg gatgaaaatg aaaatccgta
    16501 aacatgtcca cgacattttc aaaaacagta ttgatgatct ctactatcca tacgagaaaa
    16561 acagctaaaa gcagccataa ccattctatc cgatcaagtt ggaaaatcac gcccataaac
    16621 aaagcgagga ttccgaacgc aacatgtttc ctcatgttgc gttcttcctc aaaaacagtt
    16681 ttgactcctt ggacggcaaa ttccacggac atcaggaaat gtttgttttt ctctacgttt
    16741 ttatctttta agtccatagg catctaagat ctctttctgt aaaccaaaca tttctttctc
    16801 atcctctggt gtcatatggt catacccgtt gatatgcaag aatccatgaa ccgctaaaaa
    16861 gccaagttcc cgatcatacg aatggccgta agcttctgct tgctctgcag cacgttcagt
    16921 agagatcatg atgtctccca gtacacgagg caatggattc tctccatctt caaaaatcac
    16981 tgggatttcg tcttcgccct cttcttccaa ggcaaaactg atgacgtctg tcggtgcatc
    17041 ttttccacgg taatcacgat tgattacttg gattgctgca ttgtccatga aagtaacaga
    17101 catttctgtt tcttctggca actctaaata tgatgctgca aattgcagca agtcgtcgat
    17161 ttctttcatt ttctcttctg agacttggtt tgtttcatcc atgaaggtaa tatccattat
    17221 acatcgcttc tttctattct tcttgctatt cttcttgttc ttcttttatc ttctcaactt
    17281 ccgatttcat tttaggatat ttgatccgag aatggaaggt actgcttaaa ccgctgatca
    17341 atgatttttc tactttacgg atttctttta acgtcaatcc actgtcgtcc aattgtccat
    17401 cggaaatacg ctcttcaata agattatgga caaaactttc gattttttcg ctcgttggat
    17461 ggtccattgc ccgtactgcc gcttcacaac tgtcagctat attgacgatt cctgcttcac
    17521 ggctttgagg ttttggtcca ggataacgga attgctcttc cgtaacttcc gggttgcgtt
    17581 cctttgcctt aacgtagaaa taacgcatca gcgtggttcc atgatgctgt ctacaaatat
    17641 cgatcaccat ttgcggcata ttataatctt ccaatatttt ggcaccatct gtcacatgtc
    17701 caaagataat atgtttactg tcttctggca ataagaaatt gtgcggattt tccgctcctg
    17761 aaggcaagtt ctcaacgaaa aagctggcgt gtttgatctt gccgatatca tgataatagc
    17821 aagctactcg agttagtaaa gaacgtccac cgatttctgc tacagcatta gcacttagat
    17881 tggctaccat catgctatgg tgatacgtac ctggtgcttc ttccagcaac tgtttcaata
    17941 aaggatgatt gggattgctg agttcattta atacgatcac actatcatca ttgaccatca
    18001 attcaatata ggggtgcaga cctatcgtca gcaagaatga aaacagtgca cctgtgaatc
    18061 cgcagactag cgttaaccaa gtcgttgcat ccccaaagct cattccttgg tagataatca
    18121 aaatgacatc catcatcaca ggaaaaataa tgacccacat agcagcggat aacccttgtt
    18181 ctgagatccg tttgcgtctg acgatcgttg ccatgagacc agaaaataca taggacatca
    18241 aaataactgt cagaaagttc gtgccaatcg aatcataaaa aatgaaaagt gcggatactg
    18301 cttgaaaaat accagccatg atccctgctc ttcgattcaa gaaaaagctt aaaacaagtg
    18361 gagcaaaagc cgcggggtaa aacagcggaa tataagcagc ttgttctgtt tggaagagtt
    18421 ggaaaaattt cattagtacg acgcttactg acattgccgt cacgtagaaa agtacgtatt
    18481 ttgttctttc tcctgcattt tggaattgaa gtgaattata aatcagtaca ccgatttgaa
    18541 gcaaaacagc taaaaccatg gcaatgagtg ggaaaataga agtcgtttga ttcgtcaatc
    18601 ctagtaattc caattttttc atcgctgaag catcaatttg atttccttcg cgaacaataa
    18661 tttctccttg gaagatcatc actggttgta cagcttctcg tgcagattgt tttaattctt
    18721 ctgtcttttt ctcattcaat acatcattaa ctacgatgcc ttgatccact aagtagcgaa
    18781 tcatttgttg ctgctctgtt gtgacattca aatattgaat ctgatcttct gcttcttgtt
    18841 taaaagcttc caattcgctt tcacgaactt gtttactcat ctgttcgtca atgagcttca
    18901 aactttcatc cctaactgtg tgaatttccg tttcagtcat ttcaaatatc gtcgaataaa
    18961 aattgttcgg cagtttttgg taaaacgcaa cgttttctgc attcacgttt tcaaaatttt
    19021 tcttcagtgt agcaatccgc tcttctgtag taggtttcgg gatattttca tcttccttcg
    19081 ccttatctac cttgccatta taagatttgt tgatggtatc attcgtctta tcgatcaatt
    19141 cgaataattg cttgatccga ttgttctggt catctgccag atctttttga taagtatatt
    19201 ctggagtcac tgcttctgct gccagttttc ttctttgttc ggtttcttcc gtgttctcaa
    19261 tcgttttatt cgcacggata ctctcttctg ctacttggcc ttctctgaaa tcaatggcct
    19321 tttgtttgac actactaaat gtaacgccgc agacaatgat aaaaaacaag aacagaagga
    19381 aaggcaatgc tgcctttccg agtttttctt gtagtttctg cagtgtttta ttcaacatgt
    19441 gcttatcctc ctaagcaaag cttattcttg atgaagatcc gccttttcat aagctttgat
    19501 gatttcggca actacagggt gccgaacaac gtcacttgct tcaaaattaa caaaatcgat
    19561 ttttttgatt tgctttaatg ttcgttctgc atgaacaaga ccacttgtcg tcccacgagg
    19621 caagtctatc tgacttgtat ccccattgac aatcattttt gattgataac cgagacgggt
    19681 caagaacatt ttcatctgag cgactgtcgt gttttgtgct tcgtccaaga taacgaatgc
    19741 atcatccaat gtccgccccc gcatgtaagc taaaggtgcg atttcgatca cgcctcgttc
    19801 catcaatcgg ttcgtatgat ccaatccaaa aatttggtat aaagcatcat aaacaggccg
    19861 cagataagga tccacttttt ctttcagatc cccgggtaga aagcctaggt tttcccctgc
    19921 ctccacagcg ggccgcgtca aaatgatttt ttgcacttcg ccttttttta gggcagcaat
    19981 tgctaatact acggcaagaa acgttttccc tgttccggct ggccctacac caaatacgat
    20041 gtcatgtttg gctaccgatt caacgtattt cttttgaccg ctgtttttta cccgtatagg
    20101 ttttccatta cggtctttga cgatctcttc ctcatacatc tcaacaaaat aatctaatgt
    20161 acctttttgc cccattttca atgccgtcac aacatctggc gtacttacgt ggattcctcg
    20221 tttgatcaat tcctgtaatg tccgaatgac tgaggcagcc aaggaaatct gggattgctc
    20281 accaatgatc tgaatgattt ctccacgtgt atggatggtt gtttccgttg tttcctcgat
    20341 cagttttata tgcttgtcgt gtgcacctaa aagcatgctg acatcatcag cagctgtcaa
    20401 tctaatctct aaagaactag actcttccgt caaaaataga catccccttt ttttcgattc
    20461 attctttcct attttaccat actcagaaga aactatatag caaataagaa aaggttgtaa
    20521 aagagtcgct taaccatacg taccaagaaa cgttaaaaaa tagccgtttc cattcgctaa
    20581 aggtctcgtt ttgataaaaa gaagctgaga atgtaagtta tatgaaagtg gagaacaaaa
    20641 tatttcgttt cattcaacaa gtattctcta ggtatttttc ctgtttcaaa aaaagaaaac
    20701 aaaatagtta aaatattctt gcatttcaaa aaaaacaggt gtattattac gtcaacaaat
    20761 aaacatacag caaagcgtcg aggaaaacag taggctattc aacgatttat tcccagtgag
    20821 tcatgtaagt gagaaatgac aataaatcga atagacgaac atcatttccg agtcaatgtc
    20881 gaaaacgtag atcattgtgg ctgcctccat tatcaggcta gcggataact ttatcaaaaa
    20941 gcgtccgtga atgaagaagc ttttttaagc gaaaaaaggt ggtaccgttc tagaatctag
    21001 agcccttttc ccaagtggaa aagggctttt ttttatttta aggtggtggt tttcatggaa
    21061 tctgatatta gtggcattac taattgctgc aacgtttata tcgtcaatca aaatgataat
    21121 tattggagga aaaatttatg gaagaaacaa aaacttattc atggaaacaa ctattgatgg
    21181 tcagttcact tatcttcggg atgttcttcg ggtcaggtaa tctaatcttt cctgtccact
    21241 taggtcaaat ggctggcgca aactgggtca gtgctggcgt aggatttgct atctcaggtg
    21301 ctcttttccc attactagct atcttagctg tagtagtgac aaaaagtgat ggtctgtatg
    21361 acttagctaa acctgtcgga aaatggtatg ctgccttgtt cttaatcctt gttcatttga
    21421 cgatcggacc atttttcggt acaccacgga cagctgctac tgcttttgaa atggctgcta
    21481 aaccattttt gcctgaaaaa tataattcat taggtatgtt tttattctct gctcttttct
    21541 ttttacttgc ttatttcctt actgtccatc caacacggtt ggtcaaatac gtcggaaagt
    21601 atctaaactc cttgtttctt gcattattag caatcatttt tgtcgtttct ttattccatc
    21661 caatgggaaa tctaaatcaa tctgctgcag atacttatca aacaaacgct ttaacaaatg
    21721 gtgtcctaga aggttataac acggttgatg cagtagcttt gcttgcctta agtgtcactt
    21781 ttgtccatgc agtccgtgac cttggtttca aagataaaaa agtcacagaa ttaacggcaa
    21841 aagctggtag cctgagcgtt gcgctagaag tcatgatcta ttttggactt gttcttctcg
    21901 gtgcgatgag tttgaataag atgtctttat cagaaaatgg cggaactgca ctttctcaga
    21961 tcactaattt ttatttaggt aacttcggcg ctagcttctt aggagtgatg gtcacattag
    22021 gtgtcttcac taccgctatg ggactcgttg tttcttttgc gcaagatttc cataaactgt
    22081 tccctaaagt cagttatatg acatggctgc ggttaacgac tttcgtctct tttgtcgttg
    22141 caaatgctgg tttagacaat atcatccaat ggtctttacc agtattgatg cttttgtatc
    22201 cattgtcgct tgcgttgatc ttgctttcct taacagcaaa atttttccaa aagacaccat
    22261 ttgtctatca agtaacgatg ttgtttgctg ctgttcctgc cgtattggat atgctggcaa
    22321 attcgcctgc tttagtgagt caacaaagag ttgtagcaag catgctcgaa ttttatcatc
    22381 atcacgttcc ttttgctgca ttaggacttg gctggatggt cccaacgtta cttggttacg
    22441 ctggcagcct actgttttat tacgcctatc gcctatcagg gtataaacaa gaagcaaatg
    22501 aactaccaga agaataacca aatcaaacac aatttaacat aagaaatatt aaacaggaaa
    22561 aaaccagcta gatgaaagaa tcctattctt tcgtctagct attttttagt tctctttttt
    22621 acgcgcacaa aaaaactgcg gacaatgatt gtccgcagtt taaatgggct ctataatatc
    22681 tagtgttggt gacgccttct gttgccaata ctttttcttt tttctaataa acgcaaacag
    22741 acaccttgaa atcctttaga ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat
    22801 gtcctattca gagtttacca aagagttact agatattcta gacctaaatc ttatctttca
    22861 tgaagatgcc tttcgcaaag agcgaatcaa tgacgaaaca tgttttgttt ttgatggcac
    22921 attgacctat caaccagagg aatgcttcca ttgtcactat caaaataagc aaacgatcat
    22981 taaatggggc tggaaaaaag tatccatctt attgaatgat gtcagtaact acaagaccat
    23041 tctgcggatt aacaaacagc gcttcaaatg taaacactgt ggaaagactt ttttagcaga
    23101 agattcagtt agtgatcgtc gctgctccat tgctcgcaga gtcaaacaag ccattcttga
    23161 gctattaagc gaacctcttt ctatgtcttt gatcgcacgt atgaagcaca tttctccgac
    23221 cactgtcatt cgaatacttc gcagccttcg gcctaaaacc gtttctttga accagccgtt
    23281 accagaagtg gtgtgtttcg atgaattcaa atcggtgaaa aacgtctcgg gagccatgag
    23341 ctttgtcatg atggacggca agacccatca actgattgat attgtggaaa atcggcaact
    23401 aaatccttta cgcgactatt tcttgcgcta ccctagaaaa gtccgtgaac aagtacgtct
    23461 agtggtttca gatttctata caccctatcg cacattggtg aaagagtgct ttccgaatgc
    23521 ccgaatcgtc gccgatcgct ttcatattag ccaacacatc ggtcgggcct ttaccaacca
    23581 ccgaattcaa gtaatgaaga cctttaaaaa aagggatcgt cggtcgaagc acttaaagaa
    23641 atactggaga ctacttcaaa agaatgcgtg ggaattgaaa ggtcagcatc gctactggcg
    23701 tccgtcattt cgagatcatc tgacagaagc agagatagtc gatcggctat tatcttatga
    23761 tgactcctta aagcgaggct acgaagtcta ccaggatttt ctttccgcta tcagaagaca
    23821 agatgttcct gaattcgtcg cgctcctaaa agaagactac aaggagttgc cagagcacta
    23881 ccaaccagtg ttcacgacct tcaagaaata tcgaacagaa atcaaacgag cgttgcgagt
    23941 tccctattcc aacggcccta tagaatgttt gaacaaccat atcaaagtac taaaacgaat
    24001 tgcctatggc tttagaaact ttcaaaacta ccaagaacga atctttttat atcgaggaaa
    24061 atattttaag aaaactaaga acactaccca actgactaaa gctagaacaa caacacggct
    24121 agacaagata gctgtctagc cgtgtgagta tctcgattat tctattgaat ttcggctcta
    24181 tactttttga tgttggtgaa cttcaccaac atcttttttg tataaaaaaa tatagaaaag
    24241 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
    24301 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
    24361 aaattgttta actaaag
//
LOCUS       DAZKMK010000031        24348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000031 DAZKMK010000000
VERSION     DAZKMK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.031"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..903)
                     /locus_tag="ACG8V1_001274"
     CDS             complement(55..903)
                     /locus_tag="ACG8V1_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0329375.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMDIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1127..3067)
                     /gene="thrS"
                     /locus_tag="ACG8V1_001275"
     CDS             complement(1127..3067)
                     /gene="thrS"
                     /locus_tag="ACG8V1_001275"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0329376.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3404..3583)
                     /locus_tag="ACG8V1_001276"
     CDS             complement(3404..3583)
                     /locus_tag="ACG8V1_001276"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0329377.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3630..5453)
                     /locus_tag="ACG8V1_001277"
     CDS             complement(3630..5453)
                     /locus_tag="ACG8V1_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0329378.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5731..8010
                     /gene="helD"
                     /locus_tag="ACG8V1_001278"
     CDS             5731..8010
                     /gene="helD"
                     /locus_tag="ACG8V1_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0329379.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8103..8750)
                     /locus_tag="ACG8V1_001279"
     CDS             complement(8103..8750)
                     /locus_tag="ACG8V1_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329380.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8929..9648)
                     /locus_tag="ACG8V1_001280"
     CDS             complement(8929..9648)
                     /locus_tag="ACG8V1_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0329381.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9779..10930)
                     /locus_tag="ACG8V1_001281"
     CDS             complement(9779..10930)
                     /locus_tag="ACG8V1_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0329382.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11147..11887)
                     /locus_tag="ACG8V1_001282"
     CDS             complement(11147..11887)
                     /locus_tag="ACG8V1_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0329383.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11891..12214)
                     /gene="rsfS"
                     /locus_tag="ACG8V1_001283"
     CDS             complement(11891..12214)
                     /gene="rsfS"
                     /locus_tag="ACG8V1_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0329384.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12271..12864)
                     /gene="yqeK"
                     /locus_tag="ACG8V1_001284"
     CDS             complement(12271..12864)
                     /gene="yqeK"
                     /locus_tag="ACG8V1_001284"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0329385.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12848..13498)
                     /locus_tag="ACG8V1_001285"
     CDS             complement(12848..13498)
                     /locus_tag="ACG8V1_001285"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0329386.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13508..13819)
                     /gene="yhbY"
                     /locus_tag="ACG8V1_001286"
     CDS             complement(13508..13819)
                     /gene="yhbY"
                     /locus_tag="ACG8V1_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0329387.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13835..14944)
                     /gene="yqeH"
                     /locus_tag="ACG8V1_001287"
     CDS             complement(13835..14944)
                     /gene="yqeH"
                     /locus_tag="ACG8V1_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0329388.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14941..15468)
                     /locus_tag="ACG8V1_001288"
     CDS             complement(14941..15468)
                     /locus_tag="ACG8V1_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0329389.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15648..16433)
                     /locus_tag="ACG8V1_001289"
     CDS             complement(15648..16433)
                     /locus_tag="ACG8V1_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0329390.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16426..17295)
                     /gene="accD"
                     /locus_tag="ACG8V1_001290"
     CDS             complement(16426..17295)
                     /gene="accD"
                     /locus_tag="ACG8V1_001290"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0329391.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17296..18675)
                     /locus_tag="ACG8V1_001291"
     CDS             complement(17296..18675)
                     /locus_tag="ACG8V1_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0329392.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRVLSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18683..19102)
                     /gene="fabZ"
                     /locus_tag="ACG8V1_001292"
     CDS             complement(18683..19102)
                     /gene="fabZ"
                     /locus_tag="ACG8V1_001292"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0329393.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19127..19603)
                     /gene="accB"
                     /locus_tag="ACG8V1_001293"
     CDS             complement(19127..19603)
                     /gene="accB"
                     /locus_tag="ACG8V1_001293"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0329394.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19606..20841)
                     /gene="fabF"
                     /locus_tag="ACG8V1_001294"
     CDS             complement(19606..20841)
                     /gene="fabF"
                     /locus_tag="ACG8V1_001294"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0329395.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20860..21597)
                     /gene="fabG"
                     /locus_tag="ACG8V1_001295"
     CDS             complement(20860..21597)
                     /gene="fabG"
                     /locus_tag="ACG8V1_001295"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0329396.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21609..22526)
                     /gene="fabD"
                     /locus_tag="ACG8V1_001296"
     CDS             complement(21609..22526)
                     /gene="fabD"
                     /locus_tag="ACG8V1_001296"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0329397.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22633..22857)
                     /locus_tag="ACG8V1_001297"
     CDS             complement(22633..22857)
                     /locus_tag="ACG8V1_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0329398.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22910..23872)
                     /locus_tag="ACG8V1_001298"
     CDS             complement(22910..23872)
                     /locus_tag="ACG8V1_001298"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0329399.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23882..24322)
                     /locus_tag="ACG8V1_001299"
     CDS             complement(23882..24322)
                     /locus_tag="ACG8V1_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0329400.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6661 a         5424 c         4246 g         8017 t
ORIGIN      
        1 tggaaaacta cgttttgtta ttctaaaaaa cttatataaa aaatagtaag gtttttattt
       61 cgaatccata aaatattctt ggattgcttc tcgtgttttt ttcacttttt cgctaagctt
      121 ttcgttttta gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag
      181 tgaattagtt gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac
      241 atcggctatc ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatc
      301 catgcttttc gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac
      361 aagccaaagc actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc
      421 tttttcaaaa atgattggct taccgacacg agaccatttt tgcagaatat cttctactac
      481 taaagaagag gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac
      541 gactttttgg atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc
      601 attgttacca agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa
      661 accttcatcg attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact
      721 ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc
      781 tgctaattca ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc
      841 caactttttt tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct
      901 cataagttcc tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttat
      961 tcacggtcgt atactggata aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc
     1021 catcaatccc acagattcta ttgagcatct gtgtctttga ggaatacctg ttatctattg
     1081 ttcggattgc tttctatcta ctactttttt acaggaaatt tacctattag ttttctctgc
     1141 tgtaattttt gacttctgct tccatagcat caataaagat attcaaatct tctacagctg
     1201 tttctttact tccataacga cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa
     1261 caactaattg gtaagggatc ttttgtgttt gagaggcacg tatcttatag cccatttttt
     1321 cattacgatc atcaacttct acacgcaacc ccttcatttg caaacgttct ttgatttcat
     1381 aagcataatc gccatgtgca tcaacagaaa cagggatgat tgttgcttgt ataggtgcca
     1441 accatgttgg aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa cgttccattg
     1501 tagaaacaat tccgcggtgg ataactactg gacgatgcgt attttccccg tcttctccaa
     1561 catatgtcaa atcaaagcgc tctggcaata agaagtctaa ttgtatagtt gataatgttt
     1621 cttctgtacc aagagctgtt ttgacttgaa catctagttt tggtccgtaa aaggctgcct
     1681 cgccttctgc ttcaaagtaa tctagttcta attcatccat agcggctttc agcattgctt
     1741 gggctttttc ccacattgcg tcatcatcaa aatatttatc tgtattgttt ggatcgcgat
     1801 agctcaagcg gaaacgataa tctgtaatat tgaagtcagc atatactgcc accatcaatt
     1861 caagcgtacg tttaaattca tctttgattt gatcaggacg aacaaatgta tgaccatcat
     1921 tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt tcatagcgat
     1981 gcatcattcc taattcagcg atacgaattg gtaattcacg ataagaatgg atcgtgtttt
     2041 tgtagaccat catgtgatga gggcagttca ttgggcgaag aacaagcatt tcgccatctc
     2101 ccatatccat tggcgggaac atgtcttctt gataatgatc ccagtgtcca gatgttttgt
     2161 acaactcaac atctcccatt attggtgtat atacatgttg gtaacctaga ctaacttctt
     2221 tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc cccttttgga agccagaatg
     2281 gcaaaccaga acctacttct ggggaaacca tgaacaagtc aagttctttt cctagtttac
     2341 gatggtctct ttcttttgct tcttcacgga gacggatata ttctttcaaa gctttcttgt
     2401 caaaaaaggc tgtcccgtat acacgttgca tcatttgatt gtttgagttt ccacgccaat
     2461 aagcacctgc cactgataat aatttaaata cttgtatacg gccagtggat ggaacatgtg
     2521 gtccacgaca caaatcgatg aattcgcctt gttgataagc agtaatcact tcttcttctg
     2581 gcaattcact aatcagttct actttgtacg gatcacttgc aaaaatttct aatgcttcct
     2641 gttttgaaag aacacggcgt tcgatcggca gattttcttt gacgattttc atcatttctg
     2701 cttcgattgc cggcaaatct tctgcagtta cttggttttg tccattatct gtatcgtagt
     2761 aaaaccctga atcgatagct ggtccaacgc caaagtggat atttggatag aggcgacgca
     2821 tggcttgggc catcaaatga gctgctgaat gacggactaa gccaagtgca tcttcatggt
     2881 ctggtgtgat gatctcaata cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt
     2941 ttccgttcac tttaccagct aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa
     3001 ttgccaatgt tgttgttcca gactcaaatt ctttgactgc accatctgga aaagtaattt
     3061 ttatcataat agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac
     3121 aaaaaaatcc tagcatctct tgaaaagaga tactaggacg aaaagttcgt ggttccacct
     3181 aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa
     3241 caagggtttc gaaagtggtc atcctttggt tttctcctgg aaaagtttca gcctagcttt
     3301 tcctctctta tcgaagactc tcaaattcag ttggctttct catcaatcaa ttatgtgttt
     3361 atttaaccac taataaatag aaaaatcaag tgggaaacta aacttatttt ttccattccc
     3421 catttacacc gtatgttcct tttttcatct cattgatgat gacatggatg tgttcttttg
     3481 gtgcctgcgt gtttttatga acagcttccg tgatatcttt catcatattt tctaattgtt
     3541 ccggactgcg accttctact aattcgacat gtacgaatgg cataatgctc tctcctcttt
     3601 ctgatcgttc ttttttcaac tcgattctat catactatgt ttgtcttggg tactgaatca
     3661 ggaaaaatgg ctggatcttt ttttgctctc aaggcactca aaatagagac cgtatccacg
     3721 acttcttgca aaacagctcc gaataatgcg gggattagtc ccgtacttgc aattaacatc
     3781 aaaaaaatac aaataaagat gccaatcagt acagactgtt tcgctattct catcgtatct
     3841 cttgaaatca ctactgcttt tgctacacgc aagagatcat ctttcaagat gaccgcatct
     3901 gcactttcac tagcagctgt agaaccatga gcccccattg caataccgat atctgcagca
     3961 gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg caacaggtct ttgatcttta
     4021 gggatacttt ctaaaatggt cagtttatct tgaggcaagc tacgggcatg tacagaatct
     4081 aattggactt tttctgcaat cgtttgagca atggcagatc catccccggt gatcatcatc
     4141 aaatttttca catcaagttt tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc
     4201 tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata gattgctgta
     4261 ttggacgttt ctatttcttc agctgaaaca aaagaggcac gtccaatctt tacttcatga
     4321 ttattgacta ctgcttggat tccttgaccg gtcacttctt tcagttgact aacaggatac
     4381 agttggctgt ttttcacgtt gttcactaac gaacgagcta agatatggct agattcctgt
     4441 tctgcactag ctgcatacag aagtagctct tcttttttta ttccattgac tggctgaatc
     4501 gcctctactt ccaatatccc tttggtaagc gttcctgttt tatcaaaagc aatcgttttt
     4561 gtacgtgcga gcttttcaat cgttgttcca gtttttacaa cgatcccgtt tttacttgaa
     4621 cggctcattc ctgcaactaa agcaatgggc gcagctagaa tcagtggaca aggtgatgcc
     4681 actaccagta cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga aataccaccg
     4741 atcaaataag caatcaacgt gaaagggact gcataacgat cagctaaacg gacaaatcgg
     4801 gcaggctttt cttttgattc ttcgactagt ttaataatca tttgatactg gctgtctgcg
     4861 gcacgttttt cagcgatcat ttggatcgat ccgtctccat ttaaagaccc tgacataatc
     4921 tgatctccag gtttcttggt ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc
     4981 gaagattctc ctttggtcac tcgaccatct gcgggtacaa gttcgccagg cttgactagt
     5041 aactggtcat caatatttat ttcatcgatt actatatcct gcaattttcc ttctttgaat
     5101 cgatgggcaa ttcttggggt gttatctaac aaagactgca gttctttacc cgctcgttta
     5161 ttcgcgtaat cctctagact atctccgccc gtaagcatga tcaatatcat caaactagcc
     5221 cagtattctt tgacggctaa tgttgcaagg attgcggtaa tcgctaatac atctacgcca
     5281 tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc gagaatccct
     5341 ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat
     5401 aaagctatcc caccaataag caaagtaagt aaaaattttt tgaaatgtgt cattgttttt
     5461 tctcctcttt tatgtgactt agagaggtta gtaataaatt tccagctgcc tctttcatct
     5521 taagcatagt ccttgactag cactttttaa aacaataggc cttaattgaa aacaactatc
     5581 actatcagcg tttccaaaat tcatcagttc tacaaacaaa aatattcagc aaggattact
     5641 ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa ctatgctata cttttaattc
     5701 gttatttacg gcggaaggag caaacatgcg atgaatgaga aacaactaga acaaacgcac
     5761 gtggatgaaa cgatcgaact aatccagatc gaacaaaata tgctggatac aaaacaacaa
     5821 gaattgactc aggaaatgaa tcattcgaca aaagacacag ctaatcacaa gattcgagct
     5881 ggttccaatg aagcttttta tgaatctgct gttgaatatc gccaacatga gcaggaattg
     5941 cttttgaaat accatactct tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg
     6001 aaagatagtc cttattttgc ccgtatcgac tttaaagaag accgtgaaac agaaacatta
     6061 tatctaggta tcgcttcttt aagagatctg gcagaagaaa caatcgttat tgactggcgc
     6121 gcaccaattg ccaatcttta ttatgaaggt gagattggtc ccgcatttta tgaaactgaa
     6181 acagatagaa tagatgtcga tctgcagttg aaacgacaat ttaaaatcca agaaggacgt
     6241 attttatcta tggtagatac gtcggaagta atcaatgatg actttttgct agagatattg
     6301 gatgaatcat caagcaacca tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat
     6361 gcaatcattc gggacacaac aagtaaagta atgctgattg aaggaatcgc cggaagtgga
     6421 aagacatccg ctttgcttca acgtattgct tttctgttgt atcataatcg caagtggctc
     6481 gaggctgaaa acgttcttct attttcaccc aaccatttgt tttctgatta catctcgact
     6541 gtattgcctt cattaggtga aagcggcgtg cctacacaga cattcaaaaa ttatttggtg
     6601 cagctgctgc cagagttttc tttactagat gaacagcaac aagaaagtgg ttttttgcta
     6661 ggggaaaaag atccgataca agtgatgaaa agcggattga ccttagtcga tcagattgat
     6721 cgctatatcc aaggcatcac aaactacggt cccttgttcc gtgatatgaa aataaatggc
     6781 cgtacgatcc tatcaaagga atcgattcgc caatggtata aagaaacaaa tgaacaatta
     6841 cctttacatc atagactttc tctattacaa acgaagctcc tgaaaaaatt aggcggtctg
     6901 caaaaagacg aaacacgtca gcaatgggtc aaagatctgg cggaagaaca gctccaagaa
     6961 ctatacgcaa cagatccaaa tcttgaatat acagaacaaa aggagcgttc attacgtaaa
     7021 aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat
     7081 tatcaatttg tcaatataac gaaacaatat ctccactttc tacaaactgt gccactttct
     7141 cttctaacac gacataaaat cagcgagacc aggtggcagg aacatttgac aactgttcgt
     7201 acacacttgc gtcaaagaga attgcgtcaa gaggatgctg tactattctt tttactgatg
     7261 cgccgaacat atccagtgac ggttacacac aaagctcgct atatctttat tgatgaaatg
     7321 caggatttcg caccatcaca agtggcattg ctgcaagcct tgttcccaaa agcaaatttg
     7381 actttctgtg gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg
     7441 gaagaccttt tcgtggaccg tgaagtcact cgtttccaat taacgacaag ctatcgctcc
     7501 acaaaagaaa tcactgattt tgctaatcat tttattgtga aagaacatca agtagaaaca
     7561 acagcaagaa aaggagacct tcctttgctc atccaaggaa caacagatga tatcaaattg
     7621 aattggttag cagataccat tcgaacgact cagggaaaag cccggtattg gcgtacagca
     7681 atcatagcta aaacacaaga agaatgcgaa gctctttatg ctcaactgcc agacgagttg
     7741 aaacaacaag tccagctgat tttaaatgag agtgacttta tgaagcgttc cattatttta
     7801 cttccagcct ttttagcaaa aggtctggag tttgaccgag ttttcttgtg ggatgtagat
     7861 gcagaacatt tttcttctca gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg
     7921 atgcatgaat tgaccctttt tacctccaat aatgaaagtc ctttgttaaa caatatggat
     7981 atttcatctt atgagaaaaa agtactgtaa gtaaaaagac aactgctatc attaatgtta
     8041 gcagttgtct ttttatttac aataataatt gcagaaaaag taattcaatc atttaaggtc
     8101 atttattcta tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata
     8161 atcattttca gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg
     8221 ccaatcccgc cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt
     8281 gattggaaat tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct
     8341 aggtatgcac tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata
     8401 agaatttttt caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc
     8461 cgatactctt cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc
     8521 agataatttt gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg
     8581 ttcagatacc ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg
     8641 tatttttgcg ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg
     8701 taaccgctta agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc
     8761 gtccatcttc cacatacttc atatcatttc acatgatact tatgctctca tcataccaca
     8821 ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt
     8881 gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa
     8941 tgccacatta tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt
     9001 ttcaaatgtt tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat
     9061 ctcagcgatt gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa
     9121 atccgctact tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc
     9181 gatgtctttt tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa
     9241 gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt
     9301 tttcccaaat gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc
     9361 aacaatcacc attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc
     9421 atctcctgca ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata
     9481 agttttagcg cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc
     9541 tgattttgct gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc
     9601 atctttagca gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg
     9661 ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca
     9721 cgattctgtg aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct
     9781 atactcggat tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga
     9841 tttgatcact tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat
     9901 aaatagaaat ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta
     9961 gtcgaagata atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga
    10021 cataacaaga taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca
    10081 caaaatcctc aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt
    10141 cggtcatctg atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca
    10201 aatacggcca ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct
    10261 gaggcggcac aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg
    10321 cactggctat cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga
    10381 tcggatgtaa ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca
    10441 agatatgatt tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct
    10501 cagtcatttt ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta
    10561 tcctatcttg attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt
    10621 cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt
    10681 tagcaaaata gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca
    10741 caccattttg taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc
    10801 tttgcaaaaa attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg
    10861 ccatttccac atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc
    10921 agcttttcat gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa
    10981 aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac
    11041 ttctgcgacg aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta
    11101 tgttgaagat ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg
    11161 ctacaaagaa ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg
    11221 cttcgacttt cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg
    11281 tacgctcttg atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa
    11341 cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca
    11401 aaaaagcaaa attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt
    11461 gataggtgga gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt
    11521 catcaaacac ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac
    11581 aggtaattgc ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt
    11641 ggatcgatgc gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa
    11701 ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac
    11761 cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta
    11821 accattgctg ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat
    11881 aagccatatg ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt
    11941 tttccagatt atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa
    12001 gatcgatcaa tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt
    12061 tttcttcttc tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg
    12121 ctgaacagat catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa
    12181 tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat
    12241 ctatgatgat ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt
    12301 ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt
    12361 tgtttcaaat gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac
    12421 cccaggaaaa tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag
    12481 actcatctta gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt
    12541 gattcctaat tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt
    12601 ataattcaat aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc
    12661 tggtcgttct tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc
    12721 accgtatttt ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg
    12781 ttgttcgctc attctcatct gtattttttg catcaggttt tctctagaat aagcagtata
    12841 ttcattacta tagtccatat tcatacaacc cttcattttg aatataatca attactttat
    12901 ctggaacaag gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac
    12961 tgatgtcgat ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc
    13021 ctggtcgacg tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt
    13081 ttggcaaata ttctaccata tccccaccga taataaagta ataatctgta tccggattgt
    13141 tttgcgtcaa ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa
    13201 tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat
    13261 gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa
    13321 atacgcggtc aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt
    13381 gaacgggatt gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag
    13441 gcatttcttc aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg
    13501 tcactcctta tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag
    13561 gtttgtataa tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt
    13621 ctaatacttg agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga
    13681 tcagttctct tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac
    13741 cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt
    13801 gttttcctct taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata
    13861 catctacacc ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc
    13921 ctagaccggc aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct
    13981 ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt
    14041 gataaaattc gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat
    14101 aagcaataaa tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga
    14161 aaagtgtttg cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa
    14221 tgatccgcaa atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg
    14281 gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc
    14341 ctggaaactg cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat
    14401 tgataagcgt cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc
    14461 ggtattcttc gattttttct aatagttccg tcatctcatg agatttctta gcactagtta
    14521 ataatacatc tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta
    14581 atttagtttt tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg
    14641 gattgtcacc gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat
    14701 ccaccacatt gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt
    14761 cgtcatccgt caatgagaca tcttgggtct cattataatg gcgtaatctg aaacaacgct
    14821 gacaatagac ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta
    14881 attcaccggg tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg
    14941 tcattcgatt ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat
    15001 tcttttttcc caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt
    15061 tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc
    15121 gcctaccatt actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc
    15181 tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg
    15241 cgcgacacgt tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat
    15301 ttctaaaatc caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt
    15361 attgtctaaa tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat
    15421 ctgatagatg gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca
    15481 tctgcaaaaa atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca
    15541 gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg
    15601 accaaaaata aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg
    15661 aacctcttgt atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat
    15721 ctttcaatca acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg
    15781 ttgaacgttt caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta
    15841 attttcatca actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca
    15901 aatccttctg gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct
    15961 aaacctagag caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt
    16021 acctttaaat cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct
    16081 tctgctccta caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg
    16141 aacttttccg cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa
    16201 ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt
    16261 accggtttgc catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa
    16321 cgatcaccat ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc
    16381 catcgttctt tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat
    16441 ccatcctttc tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg
    16501 gggaacaatc tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc
    16561 tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct
    16621 ttggggttct gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt
    16681 cgtcgggtcc gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc
    16741 agcagagatt tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc
    16801 tgaagctgtg aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt
    16861 gatcttttct ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc
    16921 aataatgaag ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc
    16981 tgttttatct tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt
    17041 ggttacaaga tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg
    17101 ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat
    17161 gtctttcgta taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac
    17221 agatggcgag ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt
    17281 cttaaacaga gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa
    17341 ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa
    17401 atccatctga aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaagactcg
    17461 ctgcatcttc attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat
    17521 tgaatcataa tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc
    17581 catcccacct gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa
    17641 agcaggattt tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc
    17701 ttgacacaca ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat
    17761 tcctgtgacc atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa
    17821 ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac
    17881 tgctttagct gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac
    17941 tacagaagga gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc
    18001 gcctaaatga atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc
    18061 aggataaatg attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg
    18121 ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt
    18181 cccgccacca cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc
    18241 caatgcttct tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc
    18301 ttcttgcatc aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt
    18361 aggaccaata aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga
    18421 taaaaaacca aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac
    18481 ttcctgaaca ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc
    18541 tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat
    18601 ccctagttcg cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa
    18661 tactttagaa aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt
    18721 ttttaccgtc tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata
    18781 tctcaacttc aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt
    18841 ccaatccgcc gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg
    18901 ctcctgcttg agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa
    18961 aatgtccttg aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc
    19021 cttcaaccat ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt
    19081 cttttatttc ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa
    19141 tcctaaagag tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga
    19201 tcactccgtc ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga
    19261 taacttctcc tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg
    19321 gttgcagata aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg
    19381 attctttttc tttcattatt tctggagcag ttgttgcttg ttcaggtatg acattgactg
    19441 gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat
    19501 ttttattcat atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt
    19561 gatcaaattg actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg
    19621 tttcatgcac agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt
    19681 aaaacttgca ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt
    19741 ttctacattg atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc
    19801 ttcgatcccg ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta
    19861 agtatctctg aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt
    19921 ggctggtgta cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc
    19981 ttcttccatc gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt
    20041 catatggtaa gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc
    20101 gcgagcttgt gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac
    20161 aaaaccattc cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga
    20221 gagtgctgtt aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc
    20281 tccagcaaga atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga
    20341 attcgttccg ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg
    20401 caaagcaata tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac
    20461 tcgttttgca cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc
    20521 gatcccagat ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc
    20581 gctcatctcc attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg
    20641 tttgctatct tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa
    20701 ggtaatccct gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc
    20761 ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc
    20821 agtaattacc actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc
    20881 gccatcgaca ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt
    20941 ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc
    21001 cttgactttg tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc
    21061 attacatgta atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac
    21121 gccagcttta ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact
    21181 agagagatta atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt
    21241 catattgaag gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat
    21301 tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc
    21361 aagcttttct tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc
    21421 ggaaactcct acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat
    21481 ctctccgcga ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc
    21541 tttgccgatt cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac
    21601 gttcctcctt atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga
    21661 actggtattg ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact
    21721 tcgatgactt gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca
    21781 ggttgcatga cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt
    21841 gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc
    21901 gccagttttt ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga
    21961 gggatcattc ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt
    22021 tcttcgccac caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg
    22081 tacttgctag cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca
    22141 accattttcc cgcttccagc tggggcagct tctgtcatat aagcgcctct ttttgctact
    22201 aacgcaactg cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct
    22261 aaactcagtc ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac
    22321 gcgatgctga ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt
    22381 tctgtaaagc aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc
    22441 tgctttacaa cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg
    22501 ccactaaata agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga
    22561 ttcttcctat tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc
    22621 taattttaat gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt
    22681 gattccgtct tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg
    22741 gaataaatcc aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc
    22801 ttcagtttct tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat
    22861 aatatattcc tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca
    22921 tgagaatact tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt
    22981 ttcctaaggt gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag
    23041 cagatgtatt cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt
    23101 ttcgggcaat tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg
    23161 gtttgtcttt catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa
    23221 tatctctgcc agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat
    23281 ttttattttg ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt
    23341 tttctttcag caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc
    23401 caaacaacac agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac
    23461 caatcaccag tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat
    23521 aaacaaatcc tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt
    23581 tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta
    23641 aaataaaatc caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta
    23701 aaatgcataa atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc
    23761 ttgacgaaat ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa
    23821 tcttttcagg aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc
    23881 ctcacttata gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct
    23941 gttcctccgt agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat
    24001 ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat
    24061 cttcactgcg gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca
    24121 aggtcccaac agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca
    24181 tcccgattgc ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt
    24241 tcagctcaga ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta
    24301 ctgtttttaa atctggttcc atgttccacc gtccactaat tagtttga
//
LOCUS       DAZKMK010000032        24338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000032 DAZKMK010000000
VERSION     DAZKMK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.032"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..2478)
                     /locus_tag="ACG8V1_001300"
     CDS             complement(133..2478)
                     /locus_tag="ACG8V1_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0329401.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="ACG8V1_001301"
     CDS             2757..3659
                     /locus_tag="ACG8V1_001301"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0329402.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="ACG8V1_001302"
     CDS             3870..5645
                     /locus_tag="ACG8V1_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0329403.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7297)
                     /locus_tag="ACG8V1_001303"
     CDS             complement(5711..7297)
                     /locus_tag="ACG8V1_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0329404.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7548..7772
                     /locus_tag="ACG8V1_001304"
     CDS             7548..7772
                     /locus_tag="ACG8V1_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0329405.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7914..9665
                     /locus_tag="ACG8V1_001305"
     CDS             7914..9665
                     /locus_tag="ACG8V1_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0329406.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9665..11449
                     /locus_tag="ACG8V1_001306"
     CDS             9665..11449
                     /locus_tag="ACG8V1_001306"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329407.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11784..12636
                     /gene="licT"
                     /locus_tag="ACG8V1_001307"
                     /pseudo
     CDS             11784..12636
                     /gene="licT"
                     /locus_tag="ACG8V1_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12733..14619
                     /locus_tag="ACG8V1_001308"
     CDS             12733..14619
                     /locus_tag="ACG8V1_001308"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0329408.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14660..15562)
                     /locus_tag="ACG8V1_001309"
     CDS             complement(14660..15562)
                     /locus_tag="ACG8V1_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0329409.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15792..17195
                     /locus_tag="ACG8V1_001310"
     CDS             15792..17195
                     /locus_tag="ACG8V1_001310"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0329410.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17195..18628
                     /locus_tag="ACG8V1_001311"
     CDS             17195..18628
                     /locus_tag="ACG8V1_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0329411.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18773..19699
                     /gene="trxB"
                     /locus_tag="ACG8V1_001312"
     CDS             18773..19699
                     /gene="trxB"
                     /locus_tag="ACG8V1_001312"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0329412.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19912..21639
                     /locus_tag="ACG8V1_001313"
     CDS             19912..21639
                     /locus_tag="ACG8V1_001313"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0329413.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21655..22005
                     /locus_tag="ACG8V1_001314"
     CDS             21655..22005
                     /locus_tag="ACG8V1_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0329414.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22099..22959)
                     /locus_tag="ACG8V1_001315"
     CDS             complement(22099..22959)
                     /locus_tag="ACG8V1_001315"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0329415.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23051..23479
                     /locus_tag="ACG8V1_001316"
     CDS             23051..23479
                     /locus_tag="ACG8V1_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0329416.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23641..23817
                     /gene="rpsU"
                     /locus_tag="ACG8V1_001317"
     CDS             23641..23817
                     /gene="rpsU"
                     /locus_tag="ACG8V1_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0329417.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23844..24290
                     /locus_tag="ACG8V1_001318"
     CDS             23844..24290
                     /locus_tag="ACG8V1_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0329418.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5187 g         7090 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     6781 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     6841 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     6901 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     6961 tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     7021 tatactgtaa aagcatagca attagactag acatcaaaat aactgacatc aataaatatt
     7081 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     7141 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt tttccctttg
     7201 gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     7261 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     7321 tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaagatt cggtatgcct
     7381 aatttttttt cgtggtgtgt cttgctggtt aatgaaagaa attttgttct tctctaaaaa
     7441 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     7501 aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg gtatcaacag
     7561 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     7621 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     7681 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
     7741 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt tttgcgggct
     7801 gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact attcagcaaa
     7861 aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt aaaatgtcga
     7921 tttttagaaa attaggttgg ttttttcgtc aggaaaagaa acattattta attgggattt
     7981 tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
     8041 ttgatgagat cgccgataag aatttatcaa taaagatcat ttcctggtgg atcttattat
     8101 tgatccttgc agctttctta caatatcttt ttcgttatat ctggcggacg aatatttggg
     8161 gaagtgcagc tcggttggag agagaacttc ggcagcagct attcaaacat tttacagaaa
     8221 tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat gcaacgaatg
     8281 atttgaatgc aatccagaat gtagcaggag ctggaatctt gacctttgca gattcagtga
     8341 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
     8401 ttgcgctgat tcctctgcct ttgctagccg ttacttcacg agtgctgggg tcaaaactcc
     8461 acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
     8521 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctcgcggatt
     8581 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
     8641 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
     8701 gtggaaagta tgtcttggaa ggcacgatct caatcgggca gatggtctca tttattagct
     8761 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
     8821 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg
     8881 aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
     8941 ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgcg atccaagaag
     9001 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacaatc atcaaattac
     9061 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
     9121 aatattcatt gaatgcttta ttaggatcaa tcggttatgt gcctcaggat cacttcctgt
     9181 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
     9241 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg ccaaaaggtt
     9301 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa cagaggattt
     9361 cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat gcactttctg
     9421 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
     9481 aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa gaaatcattg
     9541 ttgtcgatca agggaaaatc attgaacgag gaacccacca cgagctgctt gcattaggtg
     9601 gctggtatga acgaatgtgg gaaaaacagc aattagaagc gaagatagaa ggaggcgagg
     9661 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
     9721 gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
     9781 tatcttattt gcgtttgccc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
     9841 ttttatggat gatcatctgg ctaaacagtc agctacgaca caagttattt tgttttttgc
     9901 tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
     9961 gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat ttgaaaagct
    10021 gcatacacta ggtatgcgtt attttgacca gacaccggca ggatcgaccg tttctcgagt
    10081 gacgaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg tattgactgg
    10141 gatctttgca gtcgtttctt ctttcgttgc catgtttcaa atcaatggag aaattgcctt
    10201 gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc agaaattcag
    10261 ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga
    10321 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    10381 atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    10441 gattctgtta agtccaatca tcaattttct ctatgcattg gcgattgcgt tggcgttgac
    10501 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    10561 gacctatgtc caggaatttt ttaatccaat gtctcaaatg atggactttt tgagcacctt
    10621 tacagatggg atggttgccg gtagtcgtat tttgaaaatc atggatacag aagaatatgc
    10681 accgcaacaa aatcctggag cagatcaagc gattacttca ggtaagatcg agtttcgcaa
    10741 tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt ttgtcgctaa
    10801 tccgggtgag accgttgcat tagtggggca caccggaagc ggaaaaagct cgatcatcaa
    10861 cgtattgatg cggttttacg aattttatga aggccaaatc ttgattgatg atcatgacat
    10921 tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc aagatgcctt
    10981 cttgttttat ggagatattg caggaaatat ccggatgatg aatccagcta tcacagacga
    11041 acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg aattaccaaa
    11101 aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagtggac aacgacagct
    11161 gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg atgaagcaac
    11221 agcaaatatc gatactgaaa ccgaaggatt gatccaagaa ggattggcaa aaatgcgaca
    11281 aggacggaca acgatagcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    11341 tttagtttta gacaaaggtc atatagtaga gcgtgggaat catgaaacac ttctagcgca
    11401 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagaggttgt
    11461 gacagtagca tagtagcatt ttgccctaag cgctagagtg aaaataaaac agagcttcgc
    11521 agattcttca tctgtgagcg ttagtggcgt agtcacccga tttaaaaaag acgacgacag
    11581 ctagtcaaaa gcggctaata accactaaga aagcgttgac aaaagcgttt gctttcgata
    11641 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
    11701 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta
    11761 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
    11821 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
    11881 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
    11941 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
    12001 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
    12061 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
    12121 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
    12181 ggaaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
    12241 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
    12301 acctacctgt tcactataac aatacaggat attctaaata tcgtccgcta ttactataag
    12361 attacatttg atgaagattc agtccatttt tttcgattcg tcacacactt acgttttttt
    12421 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgttt
    12481 caggttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    12541 ttagccgaga cgttcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
    12601 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaaggagg ataaattaac
    12661 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    12721 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    12781 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    12841 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    12901 cgagcgggag ggcagtatca agttgtcatc gggaatcatg tgccagtcgt gtttgaagag
    12961 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    13021 tttgatatgc ttattgatgg aatatcgggc tgtttccaac cattccttgc tatcctagct
    13081 gctgacggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    13141 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    13201 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    13261 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    13321 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    13381 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    13441 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    13501 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    13561 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    13621 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    13681 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    13741 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    13801 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    13861 attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa aaaaatattt
    13921 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    13981 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    14041 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    14101 gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag acagaaaaga
    14161 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact gaatgcagca
    14221 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    14281 ggagaagtac gtgccccttt tgacggaaca gtcatgtccc tgtttccgac gaaacatgca
    14341 atcgggatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt ggatactgtt
    14401 cagttggaag ggaaatattt tgaaagcttg gtgcagcaag gagaaagagt gaaacaaggc
    14461 gatctgttgc ttcgcttcga caaaaagaaa atcgaagagg aaggatacag tttagaaaca
    14521 cctgtgattg tcatcaatta catggattat ttggatattt tggaaaatca aggggaaacg
    14581 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga
    14641 ctaggcttaa ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca
    14701 tgatggctga gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta
    14761 aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa
    14821 tcgatgtatt gatcgtaaaa ttaccaattt tcgaatacag tcgttcgcgt gtcgtaagtc
    14881 gcaaagcggc tgtactcatt gaagatagcg ttgaatcacc gatactggtc aaagctaaaa
    14941 ttggcacatg attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg
    15001 tataagaaat cagaatccca cacatatcac ttgtgcaatt gtacgcttca tagaacgatt
    15061 caccaaaagt ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat
    15121 cttgagaaat caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca
    15181 ttagttgttt ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg
    15241 tcatctgttc taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa
    15301 aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa
    15361 aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg
    15421 tggacgggtg gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg
    15481 gagaatcgct taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt
    15541 tgattttttc acgtaataac atgtagatac cttcttcttg tttttttacg taaatcataa
    15601 cttttttgaa aatgatttgt aaacggattt atgttatata gcttaataaa gacatttttt
    15661 gacaagatat ttacaaaaat ccagaagaaa acactaactt ttgtaaatgt tactggaaag
    15721 aaacgaattt ctattgtttg ttttgtttac aaaaaagata ataaaagtgt aaaaagtaaa
    15781 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
    15841 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
    15901 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgtcagtcag atgtctcagc
    15961 gtatgtggga tgcttctccg tatgaagtca aaatctatgc aactctggat cgggaagaag
    16021 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
    16081 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
    16141 gcgggatatt caaagctttt cgtaccgttc cagtggtttt ggcaatcgtt gaagacatga
    16201 agcgtttatg tccaaatgct tggctgatta atttcgcgaa cccaagcggg atggtgacag
    16261 aagcagttat cagatatggc aaatgggata aagtgatcgg attatgcaat gtgccagtaa
    16321 tggcgatgat gacagaacca acaatgcttg gcaagaaaaa agaagagctg atctacaagt
    16381 ttgccggtct aaatcacttc cattggcaca aagtagcaga taataaagga aatgaccgca
    16441 cggcagaatt gattgatcgt ttatacgatg aagacaatgg attgccgaaa aatatctttg
    16501 acatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
    16561 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
    16621 gtacaagagc gcagcaagtg aaagaaacag aagcagagct gtttgaatta tacaaagatc
    16681 cagaattgga tcataaacct gaacaattgc aaaaacgagg tggtgcttat tattcagatg
    16741 ctgcctgtga aacgattgct tcgatctatg gaaataaaga aacacagatc gtggtatcta
    16801 caaagaatga tggagctgtt cctgatctgc cggcagattg tgtagtagaa gtcactgcct
    16861 atgttggcgc acaaggcgca cgaaatgtag cgtttggttc ccttcctgct gctgaaagag
    16921 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagcc gtgactggtg
    16981 attatggaac agctttgcaa gcgtttacga tcaatccgct tatacgatca ggaaagacag
    17041 caaaacggat catggatgag ttgtttattg cacatcagca gtatttaccg caattcaagg
    17101 aaacaatcag ccggttaaaa gcagaaggaa tcgaagtcca agatgaactg gcaagagaat
    17161 tatcaagcaa tacacatgta atggaggaag tatgatggga ttcagaaaag attttttatg
    17221 gggcggagct acagcagcca atcaatgcga gggcggttat gatgaaggcg gacgtgggtt
    17281 agcgaacgta gatgttgtgc caatcggaga agaccgtttt cctgtcatta ctggaaaaaa
    17341 gaaaatgttt gaatttgatg aaaatcattt ttatccagca aaaattgcga tcgatatgta
    17401 tcatcattat aaggaagata ttgctttatt tgcagaaatg gggttcaaga cttatcggat
    17461 gtccattgct tggagtcgta tttttccaat gggcgatgaa aaagagccaa atgaatctgg
    17521 gttgaaattt tatgaggatg tctttaaaga atgtcaaaag tacgggatcg agccgcttgt
    17581 gacgattaca cattttgatt gtccgatgca tttgatcaaa gaatatggtg cgtggcgttc
    17641 cagagaactc gttggttttt atgaaaatct gtgccgagtc atcttcaatc gctataaagg
    17701 acttgtcaaa tattggttga cattcaatga gatcaatatg atccttcatg caccattcat
    17761 gggcgcaggg ctgtattttg aagaaggaga aaatgaagaa caaatcaaat atcaagcggc
    17821 acatcatgag ttggttgcaa gtgccatcgc aacgaagatc gctcatgaag tagatccgga
    17881 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
    17941 agatgtttgg gcttctagaa agtcagatcg agaaaattat ttcttcattg atgtccagtc
    18001 acgaggagaa tatcccgctt atgcattgaa agaaatggaa cgaaatggca ttcagatcaa
    18061 aatggaacct ggggatgccg cattgctgaa agaacatact gttgatttta tctccttttc
    18121 ttattattct tctcgagttt cgactactga caacgaactc ttggaacaga ctgcaggaaa
    18181 tatctttgct tctgtcaaaa atccatatct ggaagcaagt gaatggggtt ggcaaattga
    18241 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccactatt
    18301 tattgtagaa aacggcttag gtgcagtcga tacgcccgat gagaatggtt atgtggaaga
    18361 tgattatcga atcgactatc tggcagcaca tatccaggca atgaaagatg cagtagaatt
    18421 agatggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
    18481 tacaggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgaca acgaaggaaa
    18541 tggcacatta gcccgttcga agaagaaatc attcaactgg tataaaaaag taattgattc
    18601 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
    18661 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
    18721 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
    18781 tgtaatcgtt attggcgcag ggcctgctgg gatgactgct gctttatatg cttcacgttc
    18841 aaacttatcc gtattgatga tcgaacaagg cgctcctggc ggccaaatga acaatacggc
    18901 agaaatcgaa aactatcctg gtttcgattc aatcatgggt cctgatctag cttataagat
    18961 gtatgaaggt gtttcaagat ttgggacaga aaatgcttat ggaatcgtgc aagatatcaa
    19021 ggatcacggc agccacaaag aagttatttg tgaagataaa acttacgaag gaaaaacagt
    19081 cattatcgca acaggctgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
    19141 tggacgtggc gtttcttact gtgcagtttg tgatggtgca tttttccgta acaaacgtct
    19201 agtagtgatc ggaggaggag actcagcagt agaagaagcc atctatttga cacaatttgc
    19261 ttctgaagta gtcatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
    19321 ccgtgcattt gcgaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
    19381 tggaaatgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaactg gcaatgagtt
    19441 tgatttggca gcagacggag tattcatcta tgtaggattg gatccattaa ctgcaccatt
    19501 tagagaagct gggttgacga acgaagccgg ctggattcca acagacaacg aaatgaagac
    19561 gaatatacca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
    19621 tactgctgtt ggtgatggtg ggattgctgg acaacaagtg tataaatact tggaatcttt
    19681 agaagaaaca actgcataag tttaaaaaga gtgaaagaac ggtttcaatt gttcttttac
    19741 tctttttttt gcgtgaaatc tactgaatcc gatttatgcg aatacttgta accgtgcttg
    19801 ataaaagaat cttttcttga tttaagcgtt ttttggtcct catactttca tatctaataa
    19861 gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg
    19921 gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa
    19981 ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa
    20041 ttcggtactg caggcatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat
    20101 acggtaagac aagcaacaga aggacttgca cgtttcatgg atacacaagg tgaagaaaca
    20161 aaacgccgag gtgtcgctat cgcttatgac tcacgccatc aatcaccaga atttgccatg
    20221 gaagcagcca aaacattagc acatcacaat atcccttctt atgtattcga aagtttacgt
    20281 ccaacaccag agctttcttt tgcagtccgt catttgaaga cgtttgcggg aatcatggta
    20341 actgcatccc acaatccagc agcttacaac ggatataaag tctacggaga agatggggga
    20401 caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg
    20461 ttaaaagttg aagtattatc tgacgacgaa gctgaacata gcggattgat cacgatcatc
    20521 ggagaagaag tggatgccgc ttatctagaa gaaatcaaag cagtaacgat tgatcatgaa
    20581 ttggtggaaa caatgggtaa agatttgaag cttgtgtaca caccacttca cggaacagga
    20641 aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttcca
    20701 gaacaagctg tcgcagaccc ggatttcact acagtcaaat caccgaatcc agaagaacac
    20761 tctgctttcg agtatgcgat gcgacttggc gagaaagaag gcgctgactt actgattgca
    20821 acggatcctg atgcagaccg tttaggcgca gccgttcgtt tgccaaatgg ctcctatgaa
    20881 gtattgacag gaaaccaaat cggagccctg tttgctcaat atattttgga agcacaccaa
    20941 tcgcgaggca ctttacctga aaatgcagcg gtattgaaat caatcgtttc tagcgaattg
    21001 cctacagcaa ttgcggaaca ctatggggct accatgttca atgtattgac aggattcaaa
    21061 ttcattgctg aaaaaattca acaatttgaa gaagatcatt cccatacttt tatgttcgga
    21121 ttcgaagaaa gttacggtta tttagttaaa cctttcgttc gcgacaaaga cgcgattcaa
    21181 gcattgctct tattagcaga agtagcagct tactataaaa aacaaggcaa aacattgtat
    21241 gacggcttgc aaacgatttt tgaaacatat ggctactacg cagaaaaaac gatttctgtg
    21301 acaatgagcg gacaagaagg ctctgcaaaa atctctgctt tgatggaaaa attccgtgaa
    21361 gaagcaccga cagaatttgc cggagcaaaa gtcatccaaa cagaagactt caaagagttg
    21421 acgagaacag accgtgaagg aaacgtagaa aaactaacga caccgccatc tgacgtattg
    21481 aagtatacgc tggaagaaaa tggctggatc gctatccgtc cttcaggaac tgagccgaaa
    21541 atcaaatttt atatcggtgt aaaaggaaac tcttcagaag acgcagctga aaaagtagcc
    21601 gcttatgaag cgattatcaa aaaaatgaca gaagaataaa aaaacttgag actgatggaa
    21661 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    21721 acgcaaaaaa caaaagtaga gaaagtcagc caactattca aaatgttgag tgaccccact
    21781 cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    21841 gcagtagaaa tggaacaatc tgctgtatcg catcaactgc gtttattaag agaaaaccat
    21901 gtcgtcaagt cccatcgtga gggaaaagcg attttgtata gtcttgatga ttctcacgta
    21961 ctggatattt tgaaccagac gctcaaacat gtggaacatc aataacgata ggtgcaaata
    22021 gagacaaact ggactgtatc aacgaatctt cttcgctgac acagtccagt ttttttaaga
    22081 aaaagacaag aaaaactgtt agtctgtttc ttcggtggtt tcagttccat aataactatg
    22141 atcttctagg tctaaagcat tcaaaataag tgaagctgtt tcttcgatag ataaagaagc
    22201 gacattgatt actacgcagc ccaatttttc ataaagatca ttcgcaaaat cgagttcagc
    22261 tcgtattttt tcgatatcgg aataagcagt atcaggattt aatccgtaag agcgcatccg
    22321 ttctttacgg atattgttta aaatatctgg gttattcgtc agtcccacga tttttttagg
    22381 atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta gattggcaac
    22441 ctttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc tggatacgcc
    22501 tagcaaaacg acatctgctt ctagaaaccc acgaggatct ttcccgtcat cgtattttac
    22561 agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat gaagggcgcc
    22621 aggttgccga atgggcttga taccagttaa gcgctcgatt tctgatatcg gcggagtcat
    22681 cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgcgtcatct cgatcaattc
    22741 ttgattgatc aatgtgtgta ggacaagggc tttttctttt agtgcatctt gcagtgcctg
    22801 catcaatgtt ttctcatctg tcacaaatgt tctgcggaat aaattgaact caacggtagg
    22861 atattgggcc atagtggctt gcgctaattt tgtcgcagtt tctccagcag agtcagagat
    22921 gacaaaaaaa gtaatgattt ctgtagaatc ggaagacatg gataaaacct cacttgcatt
    22981 ttttttatgt ttattatacc atactctaga atgtgtactt cgtaatcatt ttgatttgga
    23041 ggaataagtc atgaaaaaac agacattgat tgaacaatca ctagaaacat tgaaaaataa
    23101 cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga agcgcaatcg
    23161 ctacgtatcc gctaaagaag tttttgacta tatgaatcag gaattcaaag gagtaagtta
    23221 cgatacgatt tatcgaaatc tccatgattt tgcagaaatc gatttactgg aagaaaccga
    23281 tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa ggggaaatcg gccatcacca
    23341 tcactttatc tgtacggtgt gcggaacaac gaaagaaatc catatgtgtc caatgaatta
    23401 tttcgaagaa cagctcggtg gatgtacgat cgaagggcat agattcgaga ttttcggaag
    23461 atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt aaaaaaaagc
    23521 aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca gtcttgtttt
    23581 ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga gggaattaac
    23641 atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg tcgcttcaaa
    23701 cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt ctacgaaaaa
    23761 ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa attctagttc
    23821 tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat gacatgaaaa
    23881 cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc aagtcatcta
    23941 tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa ttaacggtgt
    24001 tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa gcaggacgtg
    24061 acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt cgaaaaatac atgccggaac
    24121 aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcacacaa actggtgctt
    24181 catcaatgaa agaatttggc aaagtcatgg gtgcgatcat gcctaaagtc aaaggaaaag
    24241 cagatggaaa ccaagtaaat gccattgtga aagaactatt acaatcttaa ttttatcatt
    24301 taaaggctct tcgtcaatga gtgttggtga agaaattc
//
LOCUS       DAZKMK010000033        24256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000033 DAZKMK010000000
VERSION     DAZKMK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.033"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..605)
                     /locus_tag="ACG8V1_001319"
     CDS             complement(60..605)
                     /locus_tag="ACG8V1_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0329419.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(617..1309)
                     /locus_tag="ACG8V1_001320"
     CDS             complement(617..1309)
                     /locus_tag="ACG8V1_001320"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0329420.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1328..1612)
                     /gene="macP"
                     /locus_tag="ACG8V1_001321"
     CDS             complement(1328..1612)
                     /gene="macP"
                     /locus_tag="ACG8V1_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0329421.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1625..2188)
                     /locus_tag="ACG8V1_001322"
     CDS             complement(1625..2188)
                     /locus_tag="ACG8V1_001322"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0329422.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2416..3078
                     /locus_tag="ACG8V1_001323"
     CDS             2416..3078
                     /locus_tag="ACG8V1_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0329423.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3202..4398
                     /locus_tag="ACG8V1_001324"
     CDS             3202..4398
                     /locus_tag="ACG8V1_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0329424.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4499..5875)
                     /locus_tag="ACG8V1_001325"
     CDS             complement(4499..5875)
                     /locus_tag="ACG8V1_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0329425.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5912..6778)
                     /locus_tag="ACG8V1_001326"
     CDS             complement(5912..6778)
                     /locus_tag="ACG8V1_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0329426.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6867..7892)
                     /locus_tag="ACG8V1_001327"
     CDS             complement(6867..7892)
                     /locus_tag="ACG8V1_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0329427.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7910..8584)
                     /locus_tag="ACG8V1_001328"
     CDS             complement(7910..8584)
                     /locus_tag="ACG8V1_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0329428.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8768..9661)
                     /locus_tag="ACG8V1_001329"
     CDS             complement(8768..9661)
                     /locus_tag="ACG8V1_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0329429.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9735..11432)
                     /locus_tag="ACG8V1_001330"
     CDS             complement(9735..11432)
                     /locus_tag="ACG8V1_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0329430.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11425..12738)
                     /locus_tag="ACG8V1_001331"
     CDS             complement(11425..12738)
                     /locus_tag="ACG8V1_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0329431.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12738..16679)
                     /locus_tag="ACG8V1_001332"
     CDS             complement(12738..16679)
                     /locus_tag="ACG8V1_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0329432.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16788..17246)
                     /locus_tag="ACG8V1_001333"
     CDS             complement(16788..17246)
                     /locus_tag="ACG8V1_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0329433.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17544..18062)
                     /locus_tag="ACG8V1_001334"
     CDS             complement(17544..18062)
                     /locus_tag="ACG8V1_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0329434.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18064..18680)
                     /locus_tag="ACG8V1_001335"
                     /pseudo
     CDS             complement(18064..18680)
                     /locus_tag="ACG8V1_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(18677..19465)
                     /locus_tag="ACG8V1_001336"
     CDS             complement(18677..19465)
                     /locus_tag="ACG8V1_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329435.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(19527..20438)
                     /locus_tag="ACG8V1_001337"
     CDS             complement(19527..20438)
                     /locus_tag="ACG8V1_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329436.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20479..21813)
                     /locus_tag="ACG8V1_001338"
     CDS             complement(20479..21813)
                     /locus_tag="ACG8V1_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0329437.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22021..22851
                     /locus_tag="ACG8V1_001339"
     CDS             22021..22851
                     /locus_tag="ACG8V1_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0329438.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23095..23344)
                     /locus_tag="ACG8V1_001340"
                     /pseudo
     CDS             complement(23095..23344)
                     /locus_tag="ACG8V1_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23535..23729)
                     /locus_tag="ACG8V1_001341"
     CDS             complement(23535..23729)
                     /locus_tag="ACG8V1_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329439.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         7116 a         5277 c         4202 g         7661 t
ORIGIN      
        1 gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt
       61 tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg
      121 ggatcgaaac ttgccacgga atctttgaaa atatggagtt tgtatcctag attatacgca
      181 tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg
      241 atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt
      301 ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag
      361 ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc
      421 ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga
      481 tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca
      541 gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg
      601 ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc
      661 gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt
      721 cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag
      781 cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt
      841 tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat
      901 tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata
      961 gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct gcatcaaaat
     1021 aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag
     1081 ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa
     1141 tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg
     1201 aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc
     1261 tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc
     1321 cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa
     1381 taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg
     1441 ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt
     1501 cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg
     1561 cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat
     1621 ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat
     1681 cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact
     1741 ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat
     1801 gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc
     1861 tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg
     1921 gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta
     1981 aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc
     2041 cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa
     2101 cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt
     2161 tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg
     2221 cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc
     2281 ttagattttc tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg
     2341 tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga
     2401 catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta
     2461 tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta
     2521 gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt
     2581 cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa
     2641 atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg
     2701 aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca
     2761 gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag
     2821 tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat
     2881 gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc
     2941 gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat
     3001 gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa
     3061 ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg
     3121 tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga
     3181 ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca
     3241 gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg
     3301 accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta
     3361 attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat
     3421 gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa
     3481 ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc
     3541 ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa
     3601 gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca
     3661 aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa
     3721 gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac
     3781 tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca
     3841 acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag
     3901 attgccaatg attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc
     3961 ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat
     4021 caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac
     4081 caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa
     4141 gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc
     4201 atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact
     4261 caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa
     4321 aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg
     4381 ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt
     4441 ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct
     4501 atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa
     4561 ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac
     4621 aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt
     4681 ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg
     4741 cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga
     4801 atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga
     4861 tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa
     4921 gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca
     4981 ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc
     5041 ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag
     5101 tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt
     5161 cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac
     5221 catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga
     5281 aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat
     5341 tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta
     5401 cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg
     5461 cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg
     5521 aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg
     5581 cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc actggcactc
     5641 gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa
     5701 atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata
     5761 ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt
     5821 ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag
     5881 ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga
     5941 taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt
     6001 ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa
     6061 gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta
     6121 agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac
     6181 ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca
     6241 tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga
     6301 agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc
     6361 cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa
     6421 taccgtatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg
     6481 tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact
     6541 tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga
     6601 tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca
     6661 gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac
     6721 ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa
     6781 ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag
     6841 cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg
     6901 aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct
     6961 aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta
     7021 agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg
     7081 ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc
     7141 acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag
     7201 ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag
     7261 cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct
     7321 tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac
     7381 aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt
     7441 tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact
     7501 ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct
     7561 gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt
     7621 tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg
     7681 gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga
     7741 aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc
     7801 gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc
     7861 cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga
     7921 tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc
     7981 ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc
     8041 acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa
     8101 ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc
     8161 cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt
     8221 agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat
     8281 gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat
     8341 tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat
     8401 aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt
     8461 caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct
     8521 caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg
     8581 ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa
     8641 acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg
     8701 cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat
     8761 tggtctatta tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc
     8821 aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa
     8881 gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc
     8941 ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga
     9001 ctagatcctg cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt
     9061 tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt
     9121 gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa
     9181 tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc
     9241 ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg
     9301 gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat
     9361 ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat
     9421 gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt
     9481 tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga
     9541 gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca
     9601 taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca
     9661 ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag
     9721 cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct
     9781 ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca
     9841 ggaagtgacc cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg
     9901 atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca
     9961 cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa
    10021 tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg
    10081 cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct
    10141 ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta
    10201 agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc
    10261 tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc
    10321 gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg
    10381 atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact
    10441 ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa
    10501 tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa
    10561 gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt
    10621 tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt
    10681 cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt
    10741 acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt
    10801 ttcggataca aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg
    10861 ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg
    10921 gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt
    10981 gcttcttccg tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt
    11041 ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc
    11101 cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct
    11161 cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata
    11221 ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat
    11281 gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg
    11341 gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca
    11401 ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt tttttattaa
    11461 taaaacagca cctaacaata aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt
    11521 cgtttgcggg aatacggacc attttcctga ttgacctgga ttgcttggac cactcggctc
    11581 tttcggtttg tccgttgttt cttttacggt ctcgttataa acacgtggtg ttgcggattc
    11641 ataggcagag actggaactt tcccggattc taattcagcc atctcctgtc cgttaactac
    11701 tacatattgt ggttggcctt ttgcatccac ccaaaattca gggatgatga cctcgattct
    11761 cttacttgct tcatccatct cgtaaccttc cacacctttt aattcttcaa aataataggt
    11821 gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg aaatgaattt
    11881 gcttacattt ttgtcattca aaggatcatt gtctgctggt ttgatccatt gatttttcaa
    11941 gtcattagca ggagccatat ctaaataata tttcccccct tctatcatct gatatagagc
    12001 aaaaacagca ccttctaatg gttttccttt ttcagttgtt cctctttgac gtccatactt
    12061 gaagaagtat ggatctctta gataaccgac atttttagga tagatatgaa tctcttgcag
    12121 ttcctcggtt gattctgtcg ggtgataaat tggcaaaata gcagcaattg ggatagctgt
    12181 ctgatcgata tcaacatcca atccaacttc tggatcaatc gattcttcga aaatcaaata
    12241 aactgcatcc cgatcttggt ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa
    12301 tccgccaacc gtatctttcc cagttgttac cgaagtccct atttggacaa gcctttcttc
    12361 tgtgattagc tgacggatat tgtttctgtt cgtttcagcg atttgctgga cgaattcttt
    12421 aggagtcatg ttttcagcag cttttttttc atgataataa tcggtcatgt cgtaaatggc
    12481 aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata tctgctgttt cccctgcttc
    12541 ctcagagata gccaagccat cattttcatg gtattcaaat tgatcgttat aatcgatatc
    12601 tcgaatcatc cgtttatgca acacgaattc cacgacatct tcccctgatt cttctgccga
    12661 catcgcatgt ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa
    12721 acacaacagc tttttcatta ccttgctccc tcctttttag attgtctgac aaacaaacca
    12781 taggctgctc ctgcaagcgc catgaagaat gcagcaagca ggatatagaa taatcgtccg
    12841 gatcctcctg tagcaggcaa cggattttca ggatcgttcc cgacactaag ctgtaatctg
    12901 attcgatttg tctccaaaag ttcatattct aactcttgct gtagatcagg cgtctctgct
    12961 tcttcgtttg gataatacga aatctcaact tttagtgtac ccgcttcttt tcctggaact
    13021 acccgatcat tttctatgtc aggttcttct cgataactaa tttctaaaat aaagtaacct
    13081 ggaagttttc tatagctgtc aggtgacttc acttccttga tccgatagac cccttccaac
    13141 aagtctgaag cattgaatag ggaagaatcg tttggatctg gtgccaagcc tccctcgatg
    13201 atttcatact ctcctgcact attttgtttc tcaattgaaa attctgcgcc ttcaagacgt
    13261 cggctattca tactgtcaat tttcaaaagt tccaaatcga ttggcgctag ttcatcttct
    13321 aaggttactt gtagtacacc atcgatgatt tcatagcctt ccggcaaggg agcagtttcg
    13381 tcttcgatga ccgtatcttc aatgataaac ttaccttctt cattcagtgc aaactggaac
    13441 acttgatcag atagccgata cccatttggt gcgacagatt ccgagacctc atattcacca
    13501 atagacagat gcagaggttg gtcgtaattt aaatcgacat acttttctac tcctacggca
    13561 cgagaataga agtatccgat cccaggcaaa cgtttcgatt cttctggttc taacggatta
    13621 ccagtgtatg ctcgatacag cgggaaggaa gaatccccca ctttcctgat atcgaaacct
    13681 gcaccatcta atgtatggcc ttcatggtct aattttctaa ctcggatctc ccgataatca
    13741 aacgtattgt aagcagtgaa tgctacccat aaacgatcct cttcatctgt tgtatacgtg
    13801 agatattgcc aattttcttc acctgtttgc ttgagttgga tagtccctat agcattatcg
    13861 taaagtcgca agatccatgt gtcattttca tcaggttctt tgaaaccttc tgggttctgg
    13921 ctctcaatga ttttgtattc tgcatagttg gtagtttctt cactatcctt ataaacaaat
    13981 gaatctggct ctccgattgg atgaataact ttctcaccat ccacgattac atatttcccg
    14041 tcttcatcta ctgaatagaa cgtgagtctc cctttctctt cctctacttc gttcacacca
    14101 gaaagccctt tcgctactgg atatccgatt tccagtccgt taccatctgt ttcataaagt
    14161 tcaaactctg cgccattgat tggcatatta ctgccttcaa ttagtttatt gatattcatg
    14221 tggattggct tgaattcatt tcgcatttcc agtttgacat acaactcatc atactctgtg
    14281 ccttctatgt taggctcttg tacaacagaa agcttgtgat attctccttc ataataggaa
    14341 ttcggatctt taccttccgg tccgccgact ctcgaaccag atgtatattc atctacaagc
    14401 tcgtagtgac ctaacatctc atcttcgttg gctgcatatt ttttgatttg gaattgatac
    14461 gtctcatcta ctaactgata acgtggtggt ccagcgatct ctctgatcca aaatatgtcg
    14521 ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat cgctattccg
    14581 tccgtggtag ttttacttgt ttcgattggt tcggcttccc cactctctgg attttggtca
    14641 tataatgcaa attcgactcc agccaacggc tcgccatttt gacccttctt gctgacttgg
    14701 aaattacggc ctttatgttc gtaacgaatg gaagaagatg ggaaatacat ccgtccagta
    14761 gtgatatcat tctcataatc ctccaggttc tgaatatagg ccatattgtt cactgtgcaa
    14821 aaatttccgt cttgagctcc cgcatcaagt tttacttggt acttgatcct gaccatttcc
    14881 ccttcaccta aagaaatcgg agaaaattta taagcttggt tttcccgtcc gacatacgtt
    14941 gtcggagtcg ccactacttc tggtggggct tgtccatgac gttcttcatc ccatatttct
    15001 ggctcttggt acttcatgcc gttttctcct ggatctagat ggtaaaactg aatatctgcc
    15061 gatctgccag ctccaaatgg tgagacatta aaaccagttt catcataaag tgtggcttgc
    15121 tggatcgttc tcttgaaggt agaagagata tcctccaagg ctgtaacaat gtcattcttg
    15181 ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc aatatttttc
    15241 aatacatttc gtccaatagc atctagtcgt tgtctcccgc taacgtttcc atcgattcct
    15301 aaaccaatgg agaagatact tggcgggttc tcttgatact tgtttttcag ccaatgatga
    15361 taagcaactg tgttgatcgc agatgacttt tcttgcgaag taggactgcc attcgtattg
    15421 ttattagaag aaccatcacc aacccagtga gtcgctttat tatctccttc ttttacctct
    15481 ccataacgat agtgtacatt tacttcatct tctggacggt taaagtcaga tgcataggta
    15541 actggatgag taaaccctgc attagaagta ttcccagaaa actcctcaaa atttctgaac
    15601 aaaacgtcat tatcttcagc aatcttatct tccatcacag tccagttgct ccaagctcca
    15661 ttagtgctcg tacgataact tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct
    15721 cccacaacga ttaggatttt ttctggattt ctattttctc cgccattgtc tttatacaat
    15781 gtttcatagc catttttaag ccctagtgta agcggagttc ctccgattgg tgtacgattt
    15841 aatgctgtat tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc
    15901 gaactgattt gctcatggtt attgatagct gtactattat ggtaaaaatt caccataccg
    15961 attcgaatat ccagactcgg gtcagagagc agatctttac tcatttcgtt tactgcctca
    16021 atcaacgcag catttttagt ttgactattg gtcccagcgg ttaattcact catactcgct
    16081 gacttatcta gaacaaaaac gatatcgatt ggactgattt cctttttctc gcccccgatg
    16141 acatctagat agacattgta aagggttgtt gcatcttcag gatgtagatc agatgctgtg
    16201 ctgcttggca gaacaggttc tacccatttt ttgatgtacg ccccgttata atccaagtaa
    16261 ctgttttcaa aattccgttc atttccccaa agctgaacgg tttgagctgg aacgtttcta
    16321 tcttcttcgc ctgcagttga tccattatta gtagcatagt cgtaatttcg ccagttcgct
    16381 cccccgtttg tataatcact atcatcgatc aagttctctc cgtcatatgg cgagcctttg
    16441 tctccttgat agcgttcatt gctttgtgtc caatttccta acacttttgt atagtcattc
    16501 gttgtaaact tcgggtagcg atgctgttcg ttttcaactt ctccgctagg atagatatat
    16561 tcataggggt cttgcgtagc tgttggccca agacgctcca aaataccgtt gaataactgg
    16621 cgttgaatgc gctggagtgc ttgaccgctg ggtacgattg gttcgtccag cttttccatc
    16681 tcattttctt cgctacgttg aatttcagcg gaatcttctg tatcagtttc attttcttgt
    16741 aattcttggt tatctattct ttcatcaacc accacttgaa gaggtagtta cccttcgttc
    16801 acatcgggga tcttttctgt ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa
    16861 acttttccga cataccattc ttgaccatcg atcgtctcga cttttaggtc ttctccatct
    16921 tcatcatagg aggatagacc tgttccacga acgtttttta cggtaccaat cccggcggct
    16981 gccgcatcga ttgctaaacg tattctacct tcattggcac tttccttttt ttcaaaagcc
    17041 agatgccatt caagggtttc ttctttttca atatacttcc cttcaatttt tgcatagtcc
    17101 tcatctaaga atacctcatc ctcctttgtt tctgttgact cagcagctac atccagggtc
    17161 gtcatatgat agactgactg gaaatatggt ccaaatagca aactgacagt tactaactgc
    17221 aaaagcttca attttaaccc tttcacatcc ttatactcct ttcaaaaata tttgtcttct
    17281 ttcacaacaa tcaacaaaac aatgcccatc taattataat ttaatttaat tttaatttta
    17341 aatcaaatga tatccttttt agttaaaaaa caccaattat acaacattta ttattaaaat
    17401 taaattataa aattttataa cgttctcatt taacaaaact taagttatta agacaaatga
    17461 ataaacaact acgctttcct cgaaacaaaa aaataaagca caaatgcgca tttacgcact
    17521 catgctttat tcgtacaatt aattcagata acggcagcgc ataggatgcc aataacactt
    17581 cctaaaatag ctcctcctaa aatgtcagta ggataatgta aaagtaaaaa aatcctcgaa
    17641 taagcgatca aacaagcaaa taaaaaggct aatattcctg caactggact taattcgaac
    17701 aacgcagcta ctgataaaaa ggctagcgct gtatgtcctg aaggaaaaga agaagtcttc
    17761 ggtttacttg tgtaaaggac aagccgacga tttttttcat aaggtctttt ccgtcttgtc
    17821 gtttttttga taaaaagcaa accaacaaaa aaatagatca aactgccgat aagaatggct
    17881 agaccatatt ctctagtatc aggtatcaag actaaaacag cagcaaggag caaccaaata
    17941 aaaccaccat tagagatatt actgataaat ttcaaaaaat tgatttttcg actatccgtt
    18001 ttccgttgct tggcgaactt gttgataata ctatcatcga ttttagcaat caaacgcaac
    18061 atgttattcc tcctttttaa gaaccttttc ttgcgtacga tggataaaaa gcaggacttg
    18121 aaaataaaac aagaaactga aacctaataa agcaaaatag attggtcctg caaaccagaa
    18181 tgcgacaaga aggatcaacg tgcctgccaa atagaaaagc gcgcttttga aatcattaaa
    18241 aaacaagaaa aatgcagtaa tccaagaagc ttctttttgt tccagttgaa gcaagatttt
    18301 gtaactaaag agaagcgcgc ttaatagaag caaaaacaac gtagtataca tcaatgtata
    18361 gccaattccc ccaccccaat agcggaaaat ctgataatcg atcaatagaa gaagaaaata
    18421 aacacttgct aaactatctt gctttccata aagagagaag ttctcacgaa actttttcca
    18481 gaatagtgaa ccgagctttt tctcttcatt tttttgtaat gataaatcat atacagaact
    18541 gttcgacttg cagttcctaa tgtgaaaaca ggcaagacgc acaggaacca tagtagctgt
    18601 aaatggataa gacgtggaat ccattcaatg atttctaaga aataatcatt ggctttttta
    18661 ccaatcgatc gattcatcat ccttttgaac ctcctgtcaa atccatccct tcaatgaaat
    18721 aacgttgtgc aaaaaagaac agcaaaatcg tcgggatcaa gacaagtgtt gctcctgcca
    18781 tcagataatt ccattgtgtc gttgcttggt tctgaaaaac ttgcaagcca atctgtaacg
    18841 tgtatttttc ttgatcttgg atatataaca gaggacctaa gaaatcattc caagctgcgt
    18901 tgaaggaatt gatcgcaatg gtaatcaagg caggtttcgt caatggaagc atcaaatgac
    18961 tccaaatata caaatgattc gctccgtcga tttcagcagc ttcgatcaat tcattattga
    19021 tcgataagta aaattgacgc atcagaaata tattgaacgc attgccgaag aacgatggaa
    19081 cgatcagggg caaataagta cctacccagc cgatcttgga gaataataca tattgaggga
    19141 tcatcgttac aaaccccggg atcatcatcg tagaaagaac caaagcaaac atcagctttt
    19201 tcccaggaaa atcaagcttc gcaaatccat aagcaataaa agaatttgaa aggacattac
    19261 cgatcactac tagcacagtg atgaataatg tatttcttgc atagcgccaa aaaggaaagg
    19321 cggcaatcgt atccagatag ttttcccata cgattttttc tgggaaaaag gtcggaggat
    19381 aacgaacgat ttcttccatt ggtttcaatg aagtaaacac catccaaagc aatggaatga
    19441 tcaaaatgat tcctattact gtcatgagga cataacttag tatttttttg tttgccgatt
    19501 cacactggtc tttttgaaat aatccatcat ttgccgtcac ctccagaata atagacccac
    19561 ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca cgatcagaaa caggatcatt
    19621 gacatagcca tcgcataacc catatcaaaa gctacgaacg ccttgttcca catatgcaga
    19681 ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat ttttcgtcat gacataagct
    19741 tcttgaaaaa tttggaaaga accgatcgta gaggtgatca catcgtaaaa aataatagga
    19801 gtgattaatg gcaaagtaat acttttgaac tttccccaaa cgcctgctcc atcgatttct
    19861 gctgattcat ataaagatct aggaacattt tgtaatgtag ctaagtaaag tagcattgct
    19921 cctccagaac tccatatttt catcaaaatc aacgcaggtt tcgtccattg tggatcaaac
    19981 agccaagctg gtccagggat atggaaccag cccagaaatg tgttgatcaa tcctgcagat
    20041 ggagaaagca gctgcatcca taagatgtag actgctactc ctgataagac tgccggtaaa
    20101 tagaaagctg tacggaatac actgattcct ttgatttttt gattgagtac tgtggctaaa
    20161 aacacactga aaatcgtcgt cagcggtaca ttgaagatga cgaaataaac ggtgttgtat
    20221 aacgatttcc aaaataaatc atcgtgaacg aacattcttt tgaaatttgc tagaccaatg
    20281 aaattcatct tggatgtcaa attataatcg gtgaaactac caataactga aaaaagcata
    20341 ggccctagca taaataccaa gaacccgata ataaaaggcg agataaataa ataggcccat
    20401 cttgcttctt ttttctttcg gatagataaa cgcttcaaat tttcttgcct cctctgtacc
    20461 tagacagtca aaaatggatt attgattttg cttgactaag ttttctactg ctttttgtgc
    20521 atctgccaat ccttcttttg gtgattttgt tcctaaaaga atctggtcaa tctgttcatt
    20581 cactaatgtc gtgtaatccg gagcatctaa aggaactggt gtgatcaccg tatttttaaa
    20641 gttttcgtcc gccatttgat agatcatttt tcctttatcc gttaggttgt cattattcac
    20701 taaattctca ttggctgtct tgctcgccat gatatcaaaa cttttttcgc cgaatttttc
    20761 ttgtacttct ggagaagaaa gatatttgat gaattcataa gaggcctcag gatcttttgc
    20821 tccgtatggt acttccaaga cgaatccgcc accccacgac caatggcctg aaccttcttc
    20881 tttttctggg agctgcatca cgccaaaatc aaaatcatcc ggtgcatttt ccatcaaact
    20941 agagtaataa ttgatatttt gggcacgcat ggctactaat ccggaaataa atggatcaga
    21001 tacaccagac ccaaattcgg cttctaattt attgatcgta tttttcccat aataatcttg
    21061 ccagtcttgg atccactcca aggcttccac tttattttta gtatcaatct tgactttttc
    21121 atctttgtca aaccaactag tcccgttatc tgcgtttagc gcccatacat cagcccctaa
    21181 attccataat ggataaaaac cgatgcgtgt aaattctttt ccttcttttt tatctagttt
    21241 acgtgctgtt tcttctaatt catcccaagt tgtcggtaga tcttgttcac tgattccagc
    21301 ttctttaaat atctttttat tataaaaaat gacttgtgta tctgtattga atgggatcgc
    21361 ataaggatca ccttcatatt ctactgtatc ccaaagctgc ggataaaaat ccttagaagt
    21421 gtctttttct acatatttgg agagattagt tgcttgctgt gcttctgctc tttgggcaac
    21481 ggaatttatg tcctgtacaa taacatcggg tgcatttcca gctgttattg cagataaaga
    21541 tttagtccag atgtcacccc atggctgata cttataattt acatggatct cgttttgtga
    21601 ttgattgaaa tcctcaatga tttcttcgat agcttctttt cgtgcaccgg atccccaaaa
    21661 ggaccagaaa tcaatatttt ttcttccatc acttgttttt tcagaagaat ttgctgcact
    21721 tgaactggca tcgcttgcat tacttgatcc gcatcctcct aataaaaaca atgctgaact
    21781 tgccagcaac cctgtaaaca ataatttttt catattttac ctccaaattt taatgtaacc
    21841 gatgccggtt ttaatataac gatatcattt tgttctgaaa ataacaattt ataaggtatg
    21901 attctaaaaa aattattaat gataccaata ctttatataa ttcgcgagcg ctttctttga
    21961 attttttaat ctttcgtagc agaatgagaa tgtattaaaa atagattagg aggaattttg
    22021 atgtttagac accagaaaga attgcagttt gaagcaaaac cagatcgtcc gaatccatta
    22081 atcgcgaaat atctgcagga gctgatcggc ggccaatatg gcgagatgag tgtagctatg
    22141 cagtacctgt tccaaggctg gagctgccga ggagaagaaa aatataaaga tatgctgatg
    22201 gacattgcaa cagaagaaat cggccacgtg gaaatgttgg cgacaatggt tgctcgttta
    22261 ttagagggtg cgcctgttga agatcaagaa gcagcgatgc aaaaagatcc aacaatcggt
    22321 gccattatct ctggaatgaa tccacagcac gcgatcgtag ctggcttagg acctcgtcct
    22381 gctgacagcg tgggcaatcc atggagcggt gcctatatca ttgcgagtgg caacttgtta
    22441 gcagacttta gagctaattt aaatgccgaa tcacaaggaa gacttcaagt agcacgtatt
    22501 tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt ctgttctcct tgcaagagat
    22561 agctatcatc aaaatctatg ggaatgtgca gtgaaagaat tagaagaaaa agaaggttca
    22621 atcttagtcc caagtacttt cccgcgtgac aaagaacgaa aagacattgc ttatgacttc
    22681 tataacttct cagagggaga ccaaagtagt gaaggttctt gggctagtgg caaagcactg
    22741 gatggcgaag gcgaatatca atggttgaaa gaacctaaga tcgaaggcgc tgcaccaaaa
    22801 ttagaaccag taacaccaaa aatgtatggg acacctccat tcaagaaata aatatctacc
    22861 cgtagaaaca cccgtttttt gttccacacg ctttgtgagc aaaactgact aaagtcatta
    22921 atcataacca cccttcaaaa gggtggtttt ctctagagct ataagctcaa atcaccggcc
    22981 agcgcctaaa gacgctggct ttcatatttt atgttcaagc catattgcct ttgccagttt
    23041 tgcccacctc ttaaaaggtt tatgtactac tccttagccc ttaaaaggat cgtcttactc
    23101 tcgtacactc agcttatcta acgcactatc atgtttttct tgttcttgaa tgtacttttt
    23161 gatggtcgct tcattttgtc ctacttttgt cacatagtat ccttccaccc aaaagtgtcg
    23221 atacccaaat ttatatttta gatttgcaca atctcttgat gatctcctga atattggctc
    23281 gatattgatt ataaattatt tttcttctat actttggtgt gaatacaatg tggtatttac
    23341 acatctactt tgtacgggct aaactatttc cctttattgc tccacccgca tagcaagtgg
    23401 ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa agacaataaa tctaaaaacc
    23461 cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa atgaaatgaa aaagatctct
    23521 tacggcaatc tattttattt ttttgatttt ttataaatgg aaaatgcttc aagaagtcca
    23581 agaaataaag ccgtataaaa acaagaacct atgaaatctt tatttattag atattcaaca
    23641 gaaatcccga taacactagc caacaatata tttatcagaa gtgtaaaata aggatgcttt
    23701 ttatagaata gaactagtgt tgttttcata gactctcctt tcttcatatt tccaattaat
    23761 cacaaattca cggcattact agaaaaatta aaggggcgca gaagtggaaa aatactcttc
    23821 gactttccct acaaaccagg ccatcttgtg tttaataact ataccaaatc taaggtacct
    23881 ctaaatctaa attccatttg tggcggtgac ctccaatttt taactcttct atctcccatc
    23941 cagtatacat aaccttgata cttcgaccac ttatatatcg aacacacaaa tacctatcca
    24001 ttgcaggagt actacctaac ttagttgcta cttgcatcta aataacagct gaaaagatag
    24061 gacttactct tagtaaagta tctgaactat taatttttaa actatcattt ttatcctcaa
    24121 ctacatgcac atctgttgca acttcacttt ctgtctcatc cagataaatg attaaggaat
    24181 cgggaattgc aatatcttca gctgacgctg gaatagttga gctaaattag tcatcatatc
    24241 tttattataa aataac
//
LOCUS       DAZKMK010000034        23981 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000034 DAZKMK010000000
VERSION     DAZKMK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.034"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..634
                     /locus_tag="ACG8V1_001342"
     CDS             65..634
                     /locus_tag="ACG8V1_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0329440.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(812..1252)
                     /locus_tag="ACG8V1_001343"
     CDS             complement(812..1252)
                     /locus_tag="ACG8V1_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0329441.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1410..2174
                     /gene="map"
                     /locus_tag="ACG8V1_001344"
     CDS             1410..2174
                     /gene="map"
                     /locus_tag="ACG8V1_001344"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0329442.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2206..3129
                     /locus_tag="ACG8V1_001345"
     CDS             2206..3129
                     /locus_tag="ACG8V1_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0329443.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            3205..4344
                     /locus_tag="ACG8V1_001346"
     CDS             3205..4344
                     /locus_tag="ACG8V1_001346"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0329444.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4337..5137
                     /locus_tag="ACG8V1_001347"
     CDS             4337..5137
                     /locus_tag="ACG8V1_001347"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0329445.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            5137..5964
                     /locus_tag="ACG8V1_001348"
     CDS             5137..5964
                     /locus_tag="ACG8V1_001348"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0329446.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            5969..6676
                     /locus_tag="ACG8V1_001349"
     CDS             5969..6676
                     /locus_tag="ACG8V1_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0329447.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            6776..7642
                     /gene="rfbA"
                     /locus_tag="ACG8V1_001350"
     CDS             6776..7642
                     /gene="rfbA"
                     /locus_tag="ACG8V1_001350"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0329448.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7656..8228
                     /gene="rfbC"
                     /locus_tag="ACG8V1_001351"
     CDS             7656..8228
                     /gene="rfbC"
                     /locus_tag="ACG8V1_001351"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0329449.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            8250..9278
                     /gene="rfbB"
                     /locus_tag="ACG8V1_001352"
     CDS             8250..9278
                     /gene="rfbB"
                     /locus_tag="ACG8V1_001352"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0329450.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9376..10227
                     /gene="rfbD"
                     /locus_tag="ACG8V1_001353"
     CDS             9376..10227
                     /gene="rfbD"
                     /locus_tag="ACG8V1_001353"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0329451.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            10261..12294
                     /locus_tag="ACG8V1_001354"
     CDS             10261..12294
                     /locus_tag="ACG8V1_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329452.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12338..13618
                     /locus_tag="ACG8V1_001355"
     CDS             12338..13618
                     /locus_tag="ACG8V1_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0329453.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13828..14634
                     /locus_tag="ACG8V1_001356"
     CDS             13828..14634
                     /locus_tag="ACG8V1_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0329454.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14646..15866
                     /locus_tag="ACG8V1_001357"
     CDS             14646..15866
                     /locus_tag="ACG8V1_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329455.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15856..17442
                     /locus_tag="ACG8V1_001358"
     CDS             15856..17442
                     /locus_tag="ACG8V1_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0329456.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17481..19619
                     /locus_tag="ACG8V1_001359"
     CDS             17481..19619
                     /locus_tag="ACG8V1_001359"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0329457.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(19987..20970)
                     /locus_tag="ACG8V1_001360"
     CDS             complement(19987..20970)
                     /locus_tag="ACG8V1_001360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0329458.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            21348..22739
                     /locus_tag="ACG8V1_001361"
     CDS             21348..22739
                     /locus_tag="ACG8V1_001361"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0329459.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            22755..23795
                     /locus_tag="ACG8V1_001362"
     CDS             22755..23795
                     /locus_tag="ACG8V1_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0329460.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            23814..>23981
                     /locus_tag="ACG8V1_001363"
     CDS             23814..>23981
                     /locus_tag="ACG8V1_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0329461.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLG"
BASE COUNT         7675 a         3991 c         4925 g         7390 t
ORIGIN      
        1 acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa ggagattaaa
       61 aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg cttatgaagt
      121 agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca aaatgaattg
      181 ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac aagctctttt
      241 tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata cagggaatac
      301 gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga agttgtatcg
      361 ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct cttatactta
      421 ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag agcagatcga
      481 atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga tgtcttcaag
      541 catcatccat ccaactgatg aacagattga aaagatgatc caagacagta tagatgctat
      601 cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc cacaaatcag
      661 taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag cgtatgcatt
      721 tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata cagattgaat
      781 aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt tctttagcga
      841 aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta acattttcgg
      901 ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc gatttgcaga
      961 attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc aatagatcca
     1021 attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca tcatcgtatg
     1081 tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct acttgggtac
     1141 attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca atatcggcga
     1201 tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc atgatgtgcc
     1261 tcctcgttta taattattta acaattatga attataccaa aacatcattg aaaagaaaat
     1321 aaaacctaga gacacctggg gaatgtcatt tggctaaatg tatgccgcta tgttaaaatg
     1381 agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca cgtgaaattg
     1441 aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg cgtgatttta
     1501 taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat atcgagagtc
     1561 atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact tgtatcagta
     1621 taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac ggtgatttgg
     1681 taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt tgggcctatg
     1741 tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag aaagctttgt
     1801 acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt catgctatcc
     1861 aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt catggcgttg
     1921 gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga aaagggttgc
     1981 ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga acttggaaaa
     2041 tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt tcttgccaat
     2101 atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca caaggagaag
     2161 aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa agtttggaac
     2221 aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa gcgcatcact
     2281 gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt gtctttattt
     2341 ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca agagactgta
     2401 ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg tcctgcaatc
     2461 aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt agcggcttta
     2521 tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc gtatggcgta
     2581 gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat tgtcttgttc
     2641 atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat tttggatgct
     2701 attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac tttgaagtgg
     2761 ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat tgtccctaat
     2821 gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt cggctggatg
     2881 ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat cagcggttat
     2941 cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc tacgatcatc
     3001 attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca tcaaatcaaa
     3061 aaacgtcgag atccaagccg aaaatggttc aataaattga aaactaaatt ttctcataaa
     3121 aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttata ataattatga
     3181 ctcttaatta aagtaggtgc ccatatgctt gtttctttta gtatggttat aagatttttt
     3241 ctgacaattt tattatcact gatattaaca cctgtattca aaatcatttc agtacaaaca
     3301 ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag ttccaatgcc ttcagcgggc
     3361 ggactgccta tctttatttc atttgttata tcgactttat tcttattccg taaaattatc
     3421 cctcaatcgt atattgtacc gatcttgatt gcttctttag taatcatagt cacaggagtg
     3481 ctcgatgata aatatgagct gacacctaaa caaaaaagta tagggatctt gattgcttct
     3541 cttattattt attttgtagc agatatacgt attgattcct tcacgcttcc ttttattgga
     3601 tatatccaac taggctggct tagttttccc gtaacgattt tttggatctt cggtatcacg
     3661 aatgcggtca atctgattga cggtttggat gggctggcgg cagggatttc attgatcggt
     3721 ttaattacga taggaatcat tggatatttt ttcctccatg cttctacagt gtatattcca
     3781 gttgttattt tctgtcttgt agcaagtatt atcggttttt ttccttacaa tttttatccg
     3841 gcaaagattt atttaggcga tacaggagct ctattcctag ggtttatgat ggccgtcctt
     3901 tcactacaag ggctgaagaa cgtgactttt gtttcatcga tttctttgct gatcgtcatg
     3961 ggagtgccgg taacagacac gttttttgct atcattagga gaaaagcaaa ccgtgtttct
     4021 ttttctacag cagataaaaa acatcttcat caccgactaa tcagtttagg ctttactcat
     4081 aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt tttctttcac agctatggtc
     4141 atgaattata ctggaagaat cggaacgatc atattgatta ttgccatgtt gtttgctgca
     4201 attttattat ttgaattgat tggtctgatc aatgagaaac atcagtttat tttaaaaaca
     4261 ttgcgatttt tagggaataa agagtaccgt acgcaagtaa aacagaaata tttgaagaaa
     4321 tggaaccgtc atatagatga ataacaaatt aaaccagccg atccttatta gtattgtgac
     4381 ggcaaacagt gataaaattt ttcggacact agataatttt atttcaactg taaaagatga
     4441 aacagcagta aaaatccgaa tttttgacaa caattcactt cccgagtaca aaaaaagact
     4501 aacagagtat aggaaatatc cctacatcga tatctatttt catgatgaaa attccggatt
     4561 tggttatgga cataatcaca atctgatagc tagcgattct cgctatggca ttatttgtaa
     4621 cccagatatt ttagtggatg aagagacagt tacagcattg gtcttgttat tgaaagaaca
     4681 tccggaatgt gcgatgttag cacctaagat tcttaatgaa gatggaacaa ctcagcacct
     4741 catccgtgag cagctggctg tttttgatta cgttttgcgc tttgtgcctt tcaaatgggt
     4801 caaaaaaaag tttgacaaac gactggctgt gtttgaatgc cgaggattgc ctactgatgc
     4861 taatagttat gtacggatga tctcaggtag ttttatggta gctgatattg ataaatttaa
     4921 agagataaag gggtttgatg agcgtttctt catgtacttt gaagacaacg acttgtgttt
     4981 gagttttgaa aaagcaggtt acaagttatt gtacacaccc ttttatgcag ttatccattt
     5041 ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc gtgtttttga cttctatgat
     5101 gaaatttttc aataagtggg ggtggcgttt tttctgatga aaccagaaat attagtagtc
     5161 gttgtgcttt atcagcaact attcagtcaa tctccagctt atggaccatt aagcaaagca
     5221 cttagcgaaa agcaaattca gcttattatt tacgataata gtcctcaaag gcagcaaaat
     5281 gagctgtttg aaaaagaaaa tatcgtttat catcatgatc cgcaaaatcc tggtttagcg
     5341 gtggcttata attttgcgtt gtctcaagca gatgaaggaa cacgttgtct cgtcactctg
     5401 gatcaggata cgaggctagt cgacgattat tttgaactat tgcgtaaagt aacctttaca
     5461 gatgaatgtg ttgctgcagt tccgatgatt ttttctggga gccgacaagt ttctccccta
     5521 tattcagatc gttatatcaa tcgtacagct caagcagtag aagttgaagg cccaacagct
     5581 gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg tcaaatttct aaaaaagatt
     5641 ggcgggttta atcctgcttt ttctttagat tttttggatc attggctgtt ttggaaagtg
     5701 gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa tggaacatga tctttcggtt
     5761 ttagactatg aaaaagtttc aagtaatcga taccgttcta ttttacaagc agaaaatcga
     5821 ttttatcatg aatatgatca aaggcattta tccagtcacc gacgacaatt attgttaaga
     5881 acagctaaac agttcttgac cgtcaaaaac cggcatatct ggcggatgac tttgcgctca
     5941 tatgttaaga attggaaggt gtgaattcgt gcgttcggtt tgtatagcta cctttaatgg
     6001 aggcagatat ttaagagagc agttagatag tattttatct cagctttcag cagaagatga
     6061 agtagtcatt tctgatgatg ggtcagtaga tgatacatgg gatatattaa ctgaatacgc
     6121 agaaaaagat aagcgaatcc agcttttttc ggggcctcgt caaggattga tcgctaattt
     6181 tggttatgcg atccagcaga cgaaaggaga tctaatcttt ttggcagacc aagatgatgt
     6241 ttggttggag acaaaagtga aaaagatcac tgactatttt gagcagaatc cggatcagct
     6301 agtagtagtt tctgatttaa tcattgtgga cagcgagctg caagagatcc atgattcgta
     6361 tttttcgttt cgaaattcca aagaaggatg gcttaataat gtgataagaa gtcactatat
     6421 tggtgctggt atggctttcc ggtcttcttt aaaacccgat attttgccga tcccgtcaga
     6481 agtgccgatg catgatatgt ggattggttt gctgggagga aagaagactg ggttcctaag
     6541 agaaccgtta actctgtacc gaagacatga tttaaacgcg agtgagattg aaacacattc
     6601 taaattacgg caaaaaattt cttggcgtgt tcatctgatt acggcgttga tcaaacgaaa
     6661 atggtttaaa cgttgataaa agatgtttaa atccttaata tttccatgac aaatataaca
     6721 attgatgaga taataacaaa gtgaaatttg aaatcaaaaa ggagagcaga tatccatgaa
     6781 gggaatcatt cttgctgggg gcagcggaac acgtctgtac cctttaacaa aggcaacatc
     6841 gaaacaattg atgccgattt atgacaaacc aatgatttat tatccaatgt ctactctgat
     6901 gttggcggga atcaaagaaa ttctgattat ttcaacgcca caagatacac cacgtttcaa
     6961 ggaattattt ggaaacggaa atgatttagg actgcagatc gaatatgcag tacaagaaag
     7021 tccagatgga ttggcacaag cgtttatcat tggagaagaa tttattggag atgatagtgt
     7081 ctgcttggta ttaggggaca atatttacta tggtggcggt ctgtctaaaa tgttgcaacg
     7141 agcagcttca aaagatacag gcgcgacagt atttggctat catgtcaatg atccagaacg
     7201 ctttggtgta gtagaatttg atgagaatat gcaagctttg tcgattgaag aaaagcctgc
     7261 tcagccaaaa tcaaattacg ctgtaactgg cctttatttt tatgacaatg aggtaatttc
     7321 tatcgctaaa ggtatcaaac cttcagaacg tggagaacta gagattacgg atgtcaataa
     7381 agcatatttg gataaaggaa aactatccgt agaacttatg ggacgcggat ttgcttggct
     7441 tgatacagga acacatgaat cattgcttga agcatctacc tttatcgaaa caattgaaaa
     7501 acgtcagaac ttaaaagtgg cttgtttgga agaaattgct taccgtatgg gctacatcga
     7561 taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa aatcaatacg gtcaatattt
     7621 attgaatttg gccgctgaat aaggagtcct tacagatgaa agtaacagat acaaaattaa
     7681 cagatgtcaa aatcgtagaa atggacgtct ttggcgatca tcgcggtttc ttcacagaaa
     7741 gttattcaaa agcaaaattt gcagaacatg gattgaaata tgactttgtt caagacaacc
     7801 attcactatc agctgaagct ggtgtccttc gggggcttca tttccaaaaa ggagaagctg
     7861 cacagacaaa attgattcgg gtagtcactg gagcagtttt agatgtagta gtggatatcc
     7921 gcaaaggtag cccgacttat ggtcaatggg acggatttat cttatctgaa cataatcatc
     7981 gacagttgct tgtacctaaa ggatatgccc atggattcgt tactttgaca ccaaatgtga
     8041 acttccttta taagtgtgat aattactata atgcagaagc agatgggggc atcgcatttg
     8101 atgatccaga tttagcgatt gattggccaa ttgatccatc aagagcgatt atgtcggaaa
     8161 aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga aaatccattt gtttacggag
     8221 aaatctaaga attaaggaga aattttctta tgaaaaacat cattgttacc ggaggtgccg
     8281 gattcatcgg ctctaatttc gttcattacg tggtaaacaa tcatccagaa gtgcatgtca
     8341 ctgttttgga taaactgact tatgcaggaa acaaagagaa cttggctgga cttcctagcg
     8401 atcgcgtaga attggtcgta ggagatgttg cagatgcaga attagtggac cgtctagtaa
     8461 aagaagcgga cgcagttgtt cattacgcgg cagaatccca taacgataat tcattaaaag
     8521 atcctttccc atttgtacaa acgaatatca ttggtacgta tacactttta gaagcttgcc
     8581 gcaagtatga tgtacgctat catcacgtat ctactgacga ggtttacggc gatcttccat
     8641 tacgtgaaga cctgccagga catggggaag gcgaaggaga aaaattcact gcggaaacac
     8701 catataatcc ttcaagtcca tattcttcta caaaagctgg ttcagatctg ttagtaaaag
     8761 catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa ctgttcaaac aactatggtc
     8821 cttatcagca tattgaaaaa tttattcctc gccaagtaac gaatatctta agcggtattc
     8881 gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg gatccatacg aatgatcatt
     8941 cttcagcagt gtggctgatt ttgacgaaag gcagaattgg cgaaacctat ctgatcggtg
     9001 cagacggtga agaagataat aagaccgtga tggaattgat cttagaaatg atgggtcaac
     9061 caaaagatgc gtacgaccat gtaaacgacc gtgcaggaca cgacttgcgt tatgcaatcg
     9121 attcgacaaa attacgggaa gagttaggat ggaagcctga gtttacgaac ttccgtgatg
     9181 ggttggctga cacgatcaaa tggtacgaag aacatgaaga ctggtggaaa aaagaaaaag
     9241 aggccgttga agcagcatat gccaaaaatg gacaataata cagagaaaat gtcactgtat
     9301 taatggaaaa acacatgtga ggcaggcgct cgcctgtctc ttttttaaaa taaaaatcaa
     9361 ggagcggata atcatatgtt tttattaact ggaggaaatg ggcaactagg tacagaattg
     9421 cgccatttat tagatgaaaa aaatctgcaa tatgtttcaa cagatgcaaa agacttggat
     9481 atcacagatg ccgagaaaac aatggcttac atcacagaat taaaacctga tgttattttc
     9541 cattgtgcag cttacacagc agtagataaa gcagaagaag aagcaaaaga attagatgag
     9601 aagatcaatg ttgatggtac aagaaatgta gcacttgcag caaaagctgt gaatgctcaa
     9661 ttcgtctata tcagtacaga ctatgttttc gatggaaaaa acaaaaatgt agaatacgaa
     9721 gtagacgacc aaacgaatcc actaaatgaa tatggtcgta cgaaattatt aggcgaaaaa
     9781 gcagtagaag agattttaga agactattat atcatacgaa cttcttgggt tttcgggatc
     9841 tatggacaca atttcgtctt cacgatgcaa cgcctagcgg aaacccgcga caaactgact
     9901 gtagtaaatg accagtttgg gcgaccaact tggacacgta ctttggcaga atttatggta
     9961 tatgtcatcg aagaaaaagc accatttggt atttatcatt tgtcgaacga aaatagctgt
    10021 tcttggtatg aattcgcaaa agaaatattg aaagatacag atgtagaagt tgcacctgtt
    10081 acgtcagaag aatttccgca aaaagcaaca cgtccgcagt attcagtcat gagcctgaaa
    10141 aagacagaag agttaggatt tgtcatacca acttggcaag aagcacttgc gcaaatgctg
    10201 gaagctgcca aaaaaatgaa taagtaattt ccttttatca aatagaattt ggagcatgat
    10261 atggaaaaac aaacaagaag tgcacgacga aaagaaaagc caaaagcgaa ccaggcagat
    10321 tcgaatttgg tgcttgacct gtttctaaaa ctccgctatc ttattggcgt tttagtcatc
    10381 gtggtcatcg ttacttttaa tttaaacgga agttcacttg gtgtttggga taaatatgtg
    10441 tcgcaaagag acgatgggaa aaaaactgat gtgatttttg gacagaatcg ggaagtgcgg
    10501 tcagacgaat ggctggtgca gacacctttt tacctctcac aagctgaaga ggactttccg
    10561 ttggtgaacg aggattattc attaaacgga caaaacatga tcattgcgta taattctcct
    10621 gtaaaggata ttacggttat cggaaaacca ttcaactggg gattcttttt cctcggaaga
    10681 gatcgaggac tttcctttta ttgggcgatg aaattagtcg tcatggtctt gcttggattc
    10741 gaagtactga tgatattaac gaagagaaaa aaagctctat cgctaatcgg tgcatttgcg
    10801 ctgacatttt ctccagcagt ccagtggtgg ttcatgcaac atgtgggcga tttgattttc
    10861 tttactttgg gactgatgac tgctttttat cattatttct acaatcatga taaaaaatgg
    10921 ctacgaacgt tgatgatgct tttggtggtt attttcggta ttgggtttat tttagtgatt
    10981 tacccagccc atcaagtgat gctggcgtac ttgctgattt tttattttgt tggcctgctt
    11041 atttactata gcaagaaagt ttcatgggat ctgttggatg tagtgttgat tggaggggca
    11101 gttttattta ttggcggagt catggttcat ttctggctaa cgtcaaaaga cgcattgtta
    11161 gcttctttga ataccttata tccaggaaaa cgggtaagta ctggaggaaa gtggacgatt
    11221 ggcgaatttt tttctttcct tacaaattgg aaaattcctt ttgaagatat caactattcg
    11281 aataactctg aagtggccct gttctatcac ttttttccaa ctgtattcct agcaagtcca
    11341 tttgtgttat ttggcaaaaa agatagcgaa caaaagttac tgggacgtat tttgatgatt
    11401 ttttgtctgt tttctatctt ttggattacc gttgggttgc caaaatggct agcagaaatc
    11461 actctgctgt cttatgttcc tccattaaga gccatcctta cttttagttt tgcggcttgt
    11521 ttattatcga tctggtttat cgcttatatt tggagagaag atgttattcc tatttggtat
    11581 aaattcattt tgttggcagt aaatgcatgg tcttatttct atgcgattac gcattcgaaa
    11641 atggataatt atttttcgga ttttgaaacc atcgcagttg ctgtgttggg aacagtgatt
    11701 ttagcttttg cattatttgg ctggagacgc ttgttttacc tcgcttttgc cgccttagtt
    11761 atcgtatctg gctttttcgt caatccagtc gtagaaggta caggagcgat ttttgaaaaa
    11821 acactggcaa aagaaatcca aaaaatcgat caacaagatc ctggtaaagt ttggttgtct
    11881 gaagatgaat tatataactt caccccaaca ttgggagtga aatccttcaa tacagtacgc
    11941 ttttatccag atatagctgc atggaagaaa atcgatccga aagaagaata cgagaagtat
    12001 tataatcggt atgctcatac aagagctttt atcgctaagg aagatacaaa gttcatttta
    12061 gatcgtccag atatgttttc ggtcacgttg aattttgaag acgcagaaaa attagggatc
    12121 gactatgtga tcagtaaacg accattaact gattacaacc aattatatga agcgcagttc
    12181 acgcagctat atggaccgga taaagacggt tatatgattt ttaaagtaga atatccggat
    12241 tatccagatg taccacaaac acaaactcag gaaaatcagc tagtcgtaca gtaataggct
    12301 agctgagaat aatcctgaaa tgttagtaga aaggagagtg gagataattc aagcaatcgt
    12361 acaaaaattt gtcaacaaaa tgagtgtcgg tttcttgctt ttgttactga cgctagtctt
    12421 ttttgtttgg acagcagggt taggttttcc agcaactttg tttatatata acaatagttc
    12481 attacttatc gcttttagtg tggctatttt gctcatttta aatgtttata aagcaaaagg
    12541 cgtcgatttt ctttatctag gtgttgcgct tgcccttttc attttctttt cttactttcg
    12601 ggaattaaga aatgagtctt tatttggaga cttactgttt cctttgatta ttttattttg
    12661 tttagtaatt aaatggatcg agtggacaaa atgggatcgc ggtatttatc tgattgtctt
    12721 ttcggtcttc ctcggaatca cgttatatcg tgtatttaca gagatccaag taccagaagg
    12781 acaaagcatc tgggctccag gcaattcgct ttctgatatt tggatcaata cgaatataat
    12841 cggaagttct ttgatgactc ttgctttatt gatcagtgga tttgccagtt cttttgaacg
    12901 atggtatgta cgaatcatcg gtgcagtagc agttgttgca gcttttctcg ggatttgggt
    12961 ctgccaatct agaggtgcat tagtcgcgtt gattgttttt gcgatattgg atcttttacc
    13021 taaatcgttg atgcgtgtca ttcgtgcgcc atttattgct tacacggtta cgattctttt
    13081 agctttgccg atttcttatc tggcggctgt ctctgaaaaa gtcaatttgt ttaccggtcg
    13141 agaagatatt tggcataaat tctatcagac gctaggagaa aaatctgaac aaattttact
    13201 aggaatgaaa acatttatct tccaacgagg aaaccaattt ttaggaaacc acaatagtta
    13261 taattctatt ttgaatatct atggattgat aggatttggg attgctgctt tgctgcttat
    13321 cctttttatt ggtcgcttaa cgttgaaagc agatttatcc aacgggcaga tgacttttat
    13381 ctgggccttt tttgcggtga tgatgcagtc atttatggag gatacattga cttcataccc
    13441 atgggttccg attatttata tcctgttggc tatggccgtt catcgatatg atgaaccaga
    13501 agttgcggag gaattctatg ttatggaaga ggcatatgac gatatgactt atgatgatac
    13561 atcttatgag gaacaaccag atagcgctca ttcttccagg atccaacgct atcattgata
    13621 ggcacaaaat acgagagagg ctgtgacaac cgtcgcagcc ttttttggtt tattgcaatt
    13681 gtctagcatc ttctgtaaaa tagaaacgtt agagaaaatc ttgtatcata gaaaattaag
    13741 aaagaaataa gaacgcagct cggtaccaaa aatatggtaa aatggcactt gactgtaatt
    13801 cactgaaaat cgcttggagg aacacaaatg tttaaagatt tgtatttatt tatgaaggac
    13861 atttatcaga acagaaaatt attgctacag ttttcgatca atgacttcaa atcaagatat
    13921 gcaggatcgt ttttaggaat cttatgggcg tttatcaatc cgctatttac tgtattgatt
    13981 tattggctgg tatttggtgt tggactgaaa gcacaaatga tcgatgcaaa atacccattt
    14041 atcgtatatt tagttaccgg gatgattccc tggttcttct tctcagatgc atttgcatca
    14101 actacggttg tttttcggga atatacttat ttagtaaaga aagtcgtatt caatattcgt
    14161 atcttgccca cgacgaaaat cctatccaat ctgtacaccc acttattctt cattctgatt
    14221 ggcatagctg tcgcgcttat aaatggtata tatccttcag caatgtcttt acagctgatt
    14281 tattatttat tttgcatgtg tgctttcttg acaggattga cttggctgac ggcatcgatc
    14341 caaccatttt tacctgatat catgcaatta attacgatcg tgctgcagtt gatcatgtgg
    14401 acattaccga tattatggag ccctaatcag tttgattcag gtcttgtaag catattgaaa
    14461 ttaaatccgc tgtactatgt tgttcaaggt tatcgtgagt catttttaag tgaagcttgg
    14521 ttctgggagc atggaacata tactttgtat ttctgggttt tcacgttagt aatgttcgta
    14581 atcggctctg cagtgttccg tagattgaaa ccacattttt cagatgtgct gtaaggagaa
    14641 tagctatgac agaatatgca attagactaa aagatatcac aaaatcttat aatatgtacg
    14701 caaaaccttc ggatcgattt cgtgaagccc tcaatccttt taagaaatcc tatcatgatg
    14761 tattttatgc gttgaaaaac gtgaatgtag aaataaaaaa aggtgagatg atcggtttcg
    14821 tgggagaaaa cggatcggga aaatctacaa tgttgaaaat cattacggga gtactgacac
    14881 caacatccgg cacgatggaa atcgaaggaa aaatctcagc tttgctagaa ctaggatcag
    14941 gattcaatcc tgagtattct ggctatgaaa atatctattt aaatggaatg gttctaggat
    15001 tttcaagaga agaagtcgat gaaatggtcg atgatatcat tgaatttgct gacatcggag
    15061 accatattta tcaacctgta aaaacttatt caagcgggat gttcgttcgt ctagcttttg
    15121 ctgtagcaat caatgtgaat cccgatattt tgattgtgga tgaagcattg gcagtaggcg
    15181 atcttgaatt tcaactaaaa tgtatggaaa aatttacaga aatcaagaac tcgggaaaga
    15241 cgattttatt cgtttctcat gatatcagtt cgattcgtcg tttttgtgat cgttcctatt
    15301 ggttgaaaaa cggagaagtc gtcgaatatg gcgatacgat ggaagtaacg acgaattatg
    15361 aaaacttttt aaagaaaaaa tcagttaaaa cagtggatcg gaataaaaac caagtagaac
    15421 ggttcgccgc tccagatatc gtcgacgtga cgaaagcgga attgttggat aaagacaaaa
    15481 atccggtgga tatgctggaa caaggagaaa aactatacgt taaagtaact tatgaagtaa
    15541 aagatgatac aatcaaaaat ccggttttag gaattgcgct acgaacagtc gacaattttt
    15601 atgtatgcgg acttaatact ctactagatg gtatcaagat tccttggaaa aaaggagaga
    15661 atatctttta tctggagtat gaaaagttag gtttgttagc tggtgaatat tattttgacg
    15721 ttgcggtctt tgaagaaaat gcaacggttc ctttggtata taagacaaag tacatgaact
    15781 tgtttgtcag cggttcttat atcggagaag gcatcgtggt cttagaccat aaatgggaag
    15841 aagggactca tagcaatgaa atatgatttt gaaatggacc tagatgaaca aagcagtgtg
    15901 ggaaaaattg ccgcacagat caaaccagga agtaaagttt tagagtttgg tcccggaaac
    15961 ggccgattaa cgaaacattt aatcggagca aaacaatgtg aagtgagcat tgttgagtta
    16021 gataaggaat tgttcgattt tgtcagcgaa tttgctcaag atggatttta tggtgatatc
    16081 gaaagttttg agtgggcaaa ctattatgct ggacagacat ttgattacgt cttgtttgcg
    16141 gatgttttag aacatttggt agatcctgga aaaacattga agaaagtcag agaatttttg
    16201 aatgaagaag gagagatcct gattacattc cctaacttgg cgcataattc tgtcatgatc
    16261 gatttgttca ataaccaatt gccgtgggct tcttatggtc tcctagatga aacccataat
    16321 tctttttata ctcatgatgg cttccaaaaa gtttttgaaa aagcgggatt attcattaat
    16381 attgaagatt atttgtactt agctgtaggt gatacagaat taaaatcaac ttatgaagaa
    16441 ttgccggaag ctgtacgcta tgatttcaaa atgcgaccat ttggtgaagt ctaccaatac
    16501 tttttctcat tgatgaaaca tccagtagca caaagttcca ttgcagagcc acaaaattct
    16561 aattatgtaa aagtgttaga agtaacccag caaacgaaac aagatgaaac aatccaacaa
    16621 ataccattca ataatttcac aggggaaaat gagacactaa gtttccccgt atcagaaaca
    16681 acagaaagaa tggtgtttcg cttcgcgcaa caacctagct ttatcgaatt cagcgcagaa
    16741 gctgccggag aaaaactaac atttatcgat tcgaatgctg tagtcaaaac agtaaatgat
    16801 tgttatttgt ttgatgggaa agaattacca gagttcgttc taacggatat ttctgggaaa
    16861 gaagtgacta tccattgtca ctatcgcttt atcggggaat tgacaccaac aatgaaagaa
    16921 ttgttggaaa cgattcgtcc catggctgaa gtcacaaagc agctttctga gaaaaatgac
    16981 gaattagaaa gagaaaatca tcatcttctt gaggaaaata caagactaga ccatacgtta
    17041 aaaacaacca caaatcgcta ttgcacattg ttggaaagtg atgaatggac aataaaacat
    17101 agactaggac gacgtaagaa agaaacttcg aaaaaaatcc aagaaaaaga actttctata
    17161 tgtattgacg agaaaatctg ggatgcagaa acaaaaatat taaaaatcat cggatgggga
    17221 atcgctaatt cggatcgcca gcccttgtcc tataagttat cagctgacca atcgccattt
    17281 ttcgaagcaa ttcctgtttc acgagaagaa gtcaatcaag cagaacagct cgcaaaagga
    17341 acagaagcgg gatttgagtt acgaatcctt tgcgagcaag aacgttcatt cttagtggaa
    17401 gcagtagctc aaaatggcca atcatggatc attgaaatgt aaataatgat taacttataa
    17461 agataaggag taaacgaatc gtgcaaaaaa aagaaattgc cgtatttatc gatcaaatca
    17521 ctcgtgaacg ggccactggc gatttgctta ttgtgggctg ggcaattgat gaagtgacca
    17581 aagaaatccc tacgataaag gtagaaaaag aaaatgtgat tgctgaagca acacatgtcg
    17641 taagattgga tatcaatcat ttatataatc ttgatgttaa gacccaaagc ggttttaagg
    17701 tacgtttatc agggaaaatg agaggaaaag ccatcttaga cttccaaaca gcgagacatc
    17761 aaaatggcat agctgtgaaa ttaaacggta aataccctta tgatgacggc attgaatctt
    17821 catgggaaag aaaaaaaaga ttactgaaaa aagggataaa ttatgcaagg actcatggcg
    17881 taaaaaaagc gatccgtcgg gtcaaattgg aattgaagcc aggatcgatc gactatgcag
    17941 agtggatcag tcgtcatgag aaaccagatt tgaaagagca aaggaaagaa gcacaaggat
    18001 ttgcgtaccg tccattgatt tccatactta tgcctgtcta taacgttgaa atcaaatggc
    18061 tcgaaaaatg cattgattcg gtactggatc aaacgtatga tcattgggaa ctatgtatca
    18121 gtgatgatgc ttctactgat ccagctatcc gaaagtgtct ggaatcctat caagcaaaag
    18181 atgatagaat caaagtcgtt ttccgaaaag aaaatggaca catcagtctg gcaactaatt
    18241 ctgcattgga aatagcagaa ggagaattta tagcactctt agataatgat gatgaattac
    18301 cgccatttgc attatatgaa gtggcaaaag tgctaaacgt acatccagaa ctggatgtga
    18361 tctacagtga cgaagataaa atcgatgcag atggcaatcg ctttgatccg catttcaaag
    18421 cagactggtc gcctgataca ttgatgggaa ataattatat ctctcactta ggcgtatatc
    18481 gaacaagcat tgttaaagaa cttggcggtt tccgtaaagg ctatgaaggt tctcaagatt
    18541 atgatttggt ccttcgtgtg acagaacaaa tccctgcaga tcatatttac cacattgacc
    18601 gagtcttgta tcattggcgg acgatcccag gatcaactgc aagtaatggc gaagccaaaa
    18661 gttatatcta tgattccgga gtgaaagcat tgacagatgc cctgtctaga agaaacatca
    18721 aaggaagcgt acgtcctggc agaatttctg gattttatga gattgcttat gacgtcttgc
    18781 aagaggattt ggtcagtgtg atcattccaa cgaagaatgg ctatgaagat ctaaaaactt
    18841 gtgtggattc gatcatcgaa aaaacgtctt attcaaatta tgaaatcatt attgcggata
    18901 acggaagtac agatccaaaa atgcaagaat tatttgcaga atacaagcat cagctgaaag
    18961 accgctttat cgtagaattg attgacattc cattcaatta ttctcgaatt aacaatttag
    19021 cagcagaaaa agcaaacggc aaatactttt tattcttgaa taacgataca gaagtgattg
    19081 agccagattg gatgacggtg atggtttctt atgcacagtt tgatcgaatc ggctgtgtag
    19141 gagcaaaact gttttatcca gatgatacaa cgcagcatgc aggagtatta ttaggaatcg
    19201 gtggtgttgc agggcatgca ttgaataact atgatcgaac acattgtggt tatttcggcc
    19261 gattggtcat tgacgtcaat tacttagcag tcactgcagc ttgtatgatg gtcaaggcag
    19321 ctgacttcaa tgctgttaac gggtttgatg aaacattaga agtagcattc aacgatgttg
    19381 acttgtgctt gaaagtttat gaactgggtc gttacaatgt ctatgcacat caagcagaat
    19441 tgtaccattt cgaatctaaa tcaagaggtt atgaagatac tccagaaaaa caaagacgtt
    19501 ttgctggtga aatcaaaaaa atgcaagata aatggccagc atatattgcg catgatccat
    19561 tttacaatga taatttaaca aaagaaggaa tcggcgactt ttcgttgaga ccagattaaa
    19621 ctataataaa aagctgttat gttgtgattc gctcgcacat aacggctttt tatagttttt
    19681 tgtcaaaaaa aggcagaagg tgccgactca aaaaaataag tcgaggttgg gacagaagtg
    19741 tttaactccg agaaataaga agaaattcac gaaaattgct tttcaaattt ttgtgaattt
    19801 cggcttattt aagaagaagt tgcttctgct cccaccgttt attcgttttt aaagaatgag
    19861 acaaaagtgt ttcttacttt tgtcccacgc tcttattcat gctcagagcg ccaatagata
    19921 caaaatcata gccaaagttt cacgaaccct agctgtcaaa gaatcgatat tttattttaa
    19981 tataggctat tttaaaataa atggtttaag gtattcctct acaaaagcca ctctatcaat
    20041 tatcatgggt ttaaaataag aagtgatgga gataaccaga ttgttcttag cgtcaatata
    20101 aagaacattt ccactattac caattgctga atatatttcg ttctcagaat ctaataccca
    20161 ccataaatag ccatatctca tattttgtaa gtcttctaaa gcactggttc taggtttgaa
    20221 catctcgttt atccacatgg gtggtacgat ttgttttcct ttatacatcc ctttatttag
    20281 gcataataca ccaatttttg ccagatcact tgccgataaa cacaaaccat atcctgccgt
    20341 cccgatattt agtggatcac aaaaccagac attggtttta ggagacttat ccattgtaaa
    20401 ctgcttatgt tcttcagctg tttcagcata ataattgaca tggtttggta tccctatagg
    20461 atcaaataga tacttattgg caaaatcaac agttgttagc ttacttgtct gatacaacaa
    20521 gccagttaat atgtgtaaac agacagtttg atagttgaat tcgccggtta taccttttct
    20581 tcctcccaat aaatctagtg atgtttttgt ccaatcttca cttgaacaga cttttgtcca
    20641 tggatcgcct ttacatttgt agggtgcttt cattgttagc aagtgcttga ttgtaatagc
    20701 gtaaatagtt ttctccccgc gtttcacttt atactctgga aaataatcta atactttatc
    20761 atcgatactt ttgattaatc cttgctctaa agcaatgcca atcagcaaag atacaatact
    20821 tttcgttgct gacatgatat gacaagtatc gtctttttta taaccattcc attcatctgt
    20881 ataaacttct accccatcct tatatgcgac aatttgacat atatttggtt gtcgaaaacc
    20941 aataaaatca tgcaactctt gtttattcat aagatattac ctcactttct tctataagca
    21001 taatgtcatt aaaaatcgat ttcaattttt tcgattcttc acgtaataca ggtattctta
    21061 tggactagac agcgctttgg gaagatggat tctctgaaaa atataaaggt tcactttatg
    21121 agtggataaa aaaagcagca aatacttcga cttctttaat ttaccaacat tagtgaaaat
    21181 cgattcatct ggagatttgt aatttagagt ccttgtcttg attagaggtg tataaagctt
    21241 tttattagac atttttaaat tccattacaa tttgcttata aactaggttt ttaaaggtga
    21301 ctatggtaag ataatagaga tattattttg tgattaggag tgtatatatg aataaaaatg
    21361 gagaatggag tgctgcacga cggatattca ttatattaat ggatgttatt gtatacaatg
    21421 cagcaatata tagtagtttc ttactgaagt ttcgtggaga gatcccatta cgaaatttag
    21481 aaactttcca acactcagca attattattt cagtgatttt tattgcgttg aacattttgt
    21541 tgggtgcata tgttttttat aatcgaatga tcagtgacat cgtgtttgtc acagtcatcg
    21601 gtcaggtatt gatgacacta gggatcatgg tagtgacttt tgcgggccgc tggtttgctt
    21661 ttccacgtct tgttatctta ctttcatttg ttctcggtac gcttttctta agtatctatc
    21721 gtattctcgt atataaactt tatttacgcg taagtcgaga taaaaaggtc gtcattattg
    21781 ggttagaaaa ggatgtcgca ccagcaatcc aaaattttgg ttcaaagaaa aacaacaaac
    21841 ataaagtcga agcagtcatt cttgataact attatgcaaa catcaaaaat atgattaaag
    21901 acatcgatat tgtctatctt gcttctcata tcgaagaaac tgaaaaaatc aaaatctatc
    21961 aattagttac taaaaatgaa aaaaaattgt ttttgaatac aacatttgaa aacttgacat
    22021 tgatcaatcc aaatattatg aatttcgaag atgaaagtat tattgaagca tctggattta
    22081 gaatccaacc tgaatatgaa ctattcaaac gtttgtttga tatcatcgtt tcacttgttt
    22141 tattagttat tgcttcacca tttatgttat tcacagcaat tttagtcaaa gtgacatcac
    22201 cgggaccaat catctataaa caagtgcgga tcacaaaaaa tcaaaaggaa ttttcaatct
    22261 ataaattccg ctcgatgacc gccacagccg aagcaaaatc tggaccagtt ttagctaaga
    22321 gcaatgatgc acgagtaacg cctgtaggta aatttattcg agctgtccgc tttgatgaat
    22381 taccacaaat cttcaatgtg ctgaaaggcg atatgtcgat cgtaggacca cgtcctgaac
    22441 gtcctttctt cgtcgatcag ttcaatgcag aaaaccctta ttactactta cggcataatg
    22501 ttcgtgctgg tatcactggg tatgcacaag tctatggaaa atatgtctct gattacaaca
    22561 gtaaattacg ttttgattta ctttatatca aaaaatattc attgatgctt gatttaaaga
    22621 tcttgctgca aaccatcaag atcctatttg ataaaatgtc ctctcaagga cttgaagaag
    22681 acgaaaatgt gatctcattg gcagaaattg cttttccgaa tgaaaaaatc tttcgctagt
    22741 tgggaggcgt aaaaatgaac aatccgaaat tcagcataat cgtaccagta tacaatgcag
    22801 aagcaactat tggtcgaacg attgatattc taaaagaatt ggattatgaa gagtacgaag
    22861 tcgttttagt aaatgatgga tcaacagatc aaacacagga aatcattcaa gaactgattg
    22921 aaaatgatga acgttttgaa ttgattacta cgaccaatca agggcccgga ttagcaagaa
    22981 acgaaggaat aagacagtca aaaggagaat atctcttatt cttcgatgca gatgacacgc
    23041 cagtaaaaac gatcttgtca gattatgatc atttgctaga aaagagtcca aaagcggatc
    23101 ttgttgttag ttcgtttgtt tttcgtacaa tggacgaggg aagaatcgtt tccgaaaaag
    23161 aaaacttagt tacagaatgt cactatctaa ctcacgaaga gtttataaaa gacatgtatc
    23221 agttaatgaa tgaacagttg atgtatgttg tatggaacaa atgctatcgt agcgatattc
    23281 tgaaaaataa ccaaattcta tttaaaactt ataatagttg tgaggatcga atatttaact
    23341 tacattatta tgaatactgt catcaagtga tcatgaaccc aaaagtagaa tatatttatg
    23401 aatttgaagg tgggaagggg atcactaatc aataccgacc taacaagttc gaaacattta
    23461 aagaattcta ttcattagca aatactgtaa cagacgatat taataaagac ggtatggcgg
    23521 cgcttctttt aaaagggaca acttcagtta ttttttctat ttatagtacc tctgcaagaa
    23581 cttcaaaaga gaagaaagac gaagcaaagc agatattgag tgatccagtg atccgagaag
    23641 ccaagcaaat tgctaagacg gattcttcag caaaaaaaat aacaaagttg ctttacaata
    23701 tgccagctcc actattttta tctgctgtaa aagttggaag cttcgttgaa gtgaaaatgc
    23761 cagggttaat ggctgctttg aagcgagtat attagttaat ggaggaaaat ataatgaaaa
    23821 gaattttgca ttttcaggga cgaatgggtt taggtggagc agagtctttc atgatgaatt
    23881 tatatcgtaa aattgatcgt acaaactatc aatttgattt tttgatttat gaagattatg
    23941 cagatgttca agactatcat tcagagattg aaagattagg a
//
LOCUS       DAZKMK010000035        23826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000035 DAZKMK010000000
VERSION     DAZKMK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.035"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG8V1_001364"
     CDS             complement(55..789)
                     /locus_tag="ACG8V1_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0329462.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG8V1_001365"
     CDS             1116..1526
                     /locus_tag="ACG8V1_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0329463.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG8V1_001366"
     CDS             1519..2220
                     /locus_tag="ACG8V1_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0329464.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG8V1_001367"
     CDS             2220..3806
                     /locus_tag="ACG8V1_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329465.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG8V1_001368"
     CDS             3825..4046
                     /locus_tag="ACG8V1_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0329466.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG8V1_001369"
     CDS             4043..4804
                     /locus_tag="ACG8V1_001369"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0329467.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG8V1_001370"
     CDS             5184..5693
                     /locus_tag="ACG8V1_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0329468.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG8V1_001371"
     CDS             complement(5768..6307)
                     /locus_tag="ACG8V1_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0329469.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8V1_001372"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8V1_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0329470.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG8V1_001373"
     CDS             7320..8171
                     /locus_tag="ACG8V1_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0329471.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG8V1_001374"
     CDS             8199..8834
                     /locus_tag="ACG8V1_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0329472.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG8V1_001375"
     CDS             8854..9627
                     /locus_tag="ACG8V1_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0329473.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG8V1_001376"
     CDS             10035..10832
                     /locus_tag="ACG8V1_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0329474.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG8V1_001377"
     CDS             10810..11223
                     /locus_tag="ACG8V1_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0329475.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8V1_001378"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8V1_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0329476.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8V1_001379"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8V1_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0329477.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG8V1_001380"
     CDS             16000..16560
                     /locus_tag="ACG8V1_001380"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0329478.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8V1_001381"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8V1_001381"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0329479.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG8V1_001382"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG8V1_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0329480.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8V1_001383"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8V1_001383"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0329481.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8V1_001384"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8V1_001384"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0329482.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8V1_001385"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8V1_001385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0329483.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG8V1_001386"
     CDS             21907..22485
                     /locus_tag="ACG8V1_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0329484.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG8V1_001387"
     CDS             22537..23019
                     /locus_tag="ACG8V1_001387"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0329485.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG8V1_001388"
     CDS             23136..23510
                     /locus_tag="ACG8V1_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329486.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4286 c         5116 g         6642 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaa
//
LOCUS       DAZKMK010000036        23778 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000036 DAZKMK010000000
VERSION     DAZKMK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.036"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8V1_001389"
     CDS             complement(164..388)
                     /locus_tag="ACG8V1_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0329487.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8V1_001390"
     CDS             complement(586..2166)
                     /locus_tag="ACG8V1_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0329488.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8V1_001391"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8V1_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0329489.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8V1_001392"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8V1_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0329490.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8V1_001393"
     CDS             5331..5765
                     /locus_tag="ACG8V1_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0329491.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8V1_001394"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8V1_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0329492.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8V1_001395"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8V1_001395"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0329493.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8V1_001396"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8V1_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0329494.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8V1_001397"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8V1_001397"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0329495.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8V1_001398"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8V1_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0329496.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG8V1_001399"
     CDS             11001..11471
                     /locus_tag="ACG8V1_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0329497.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG8V1_001400"
     CDS             complement(11579..12805)
                     /locus_tag="ACG8V1_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0329498.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG8V1_001401"
     CDS             complement(13559..13759)
                     /locus_tag="ACG8V1_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0329499.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG8V1_001402"
     CDS             complement(13893..14501)
                     /locus_tag="ACG8V1_001402"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0329500.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG8V1_001403"
     CDS             complement(14586..15056)
                     /locus_tag="ACG8V1_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0329501.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG8V1_001404"
     CDS             complement(15104..16018)
                     /locus_tag="ACG8V1_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0329502.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG8V1_001405"
     CDS             16164..16967
                     /locus_tag="ACG8V1_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0329503.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG8V1_001406"
     CDS             17145..18092
                     /locus_tag="ACG8V1_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0329504.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACG8V1_001407"
     CDS             complement(18160..18453)
                     /locus_tag="ACG8V1_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0329505.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACG8V1_001408"
     CDS             complement(18481..19146)
                     /locus_tag="ACG8V1_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0329506.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACG8V1_001409"
     CDS             complement(19286..19918)
                     /locus_tag="ACG8V1_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0329507.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8V1_001410"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8V1_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0329508.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8V1_001411"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8V1_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0329509.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8V1_001412"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8V1_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0329510.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8V1_001413"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8V1_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0329511.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         7054 a         4830 c         3826 g         8068 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgta
//
LOCUS       DAZKMK010000037        23632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000037 DAZKMK010000000
VERSION     DAZKMK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.037"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8V1_001414"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8V1_001414"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0329512.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="ACG8V1_001415"
     CDS             complement(1171..1866)
                     /locus_tag="ACG8V1_001415"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0329513.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="ACG8V1_001416"
     CDS             complement(1879..2595)
                     /locus_tag="ACG8V1_001416"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0329514.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="ACG8V1_001417"
     CDS             2925..3533
                     /locus_tag="ACG8V1_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0329515.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACG8V1_001418"
     CDS             3858..4181
                     /locus_tag="ACG8V1_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0329516.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACG8V1_001419"
     CDS             4363..5733
                     /locus_tag="ACG8V1_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0329517.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACG8V1_001420"
     CDS             complement(5863..7164)
                     /locus_tag="ACG8V1_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0329518.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9509)
                     /locus_tag="ACG8V1_001421"
     CDS             complement(7518..9509)
                     /locus_tag="ACG8V1_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0329519.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="ACG8V1_001422"
     CDS             complement(9653..9919)
                     /locus_tag="ACG8V1_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0329520.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8V1_001423"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8V1_001423"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0329521.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8V1_001424"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8V1_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0329522.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /gene="def"
                     /locus_tag="ACG8V1_001425"
     CDS             13263..13826
                     /gene="def"
                     /locus_tag="ACG8V1_001425"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0329523.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="ACG8V1_001426"
     CDS             complement(14079..14600)
                     /locus_tag="ACG8V1_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0329524.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="ACG8V1_001427"
     CDS             complement(14603..15445)
                     /locus_tag="ACG8V1_001427"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0329525.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="ACG8V1_001428"
     CDS             complement(15438..16163)
                     /locus_tag="ACG8V1_001428"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0329526.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="ACG8V1_001429"
     CDS             16339..16635
                     /locus_tag="ACG8V1_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329527.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16891..17065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17203..18795
                     /locus_tag="ACG8V1_001430"
     CDS             17203..18795
                     /locus_tag="ACG8V1_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0329528.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="ACG8V1_001431"
     CDS             18808..19548
                     /locus_tag="ACG8V1_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329529.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="ACG8V1_001432"
     CDS             19716..21065
                     /locus_tag="ACG8V1_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0329530.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="ACG8V1_001433"
     CDS             complement(21193..21783)
                     /locus_tag="ACG8V1_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0329531.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8V1_001434"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8V1_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0329532.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6763 a         4759 c         4501 g         7609 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt tt
//
LOCUS       DAZKMK010000038        23507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000038 DAZKMK010000000
VERSION     DAZKMK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.038"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1813
                     /locus_tag="ACG8V1_001435"
     CDS             152..1813
                     /locus_tag="ACG8V1_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0329533.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1883..2230)
                     /locus_tag="ACG8V1_001436"
     CDS             complement(1883..2230)
                     /locus_tag="ACG8V1_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0329534.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2314..3255
                     /gene="opp3b"
                     /locus_tag="ACG8V1_001437"
     CDS             2314..3255
                     /gene="opp3b"
                     /locus_tag="ACG8V1_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0329535.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3256..4299
                     /locus_tag="ACG8V1_001438"
     CDS             3256..4299
                     /locus_tag="ACG8V1_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0329536.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4305..5375
                     /locus_tag="ACG8V1_001439"
     CDS             4305..5375
                     /locus_tag="ACG8V1_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329537.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5365..6318
                     /locus_tag="ACG8V1_001440"
     CDS             5365..6318
                     /locus_tag="ACG8V1_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329538.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6330..7340
                     /locus_tag="ACG8V1_001441"
     CDS             6330..7340
                     /locus_tag="ACG8V1_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0329539.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7625..8446)
                     /locus_tag="ACG8V1_001442"
     CDS             complement(7625..8446)
                     /locus_tag="ACG8V1_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0329540.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8543..8821)
                     /locus_tag="ACG8V1_001443"
     CDS             complement(8543..8821)
                     /locus_tag="ACG8V1_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329541.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9089..11098
                     /gene="metG"
                     /locus_tag="ACG8V1_001444"
     CDS             9089..11098
                     /gene="metG"
                     /locus_tag="ACG8V1_001444"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0329542.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11577..12164)
                     /locus_tag="ACG8V1_001445"
     CDS             complement(11577..12164)
                     /locus_tag="ACG8V1_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329543.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12339..13661
                     /locus_tag="ACG8V1_001446"
     CDS             12339..13661
                     /locus_tag="ACG8V1_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0329544.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13816..14592
                     /locus_tag="ACG8V1_001447"
     CDS             13816..14592
                     /locus_tag="ACG8V1_001447"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0329545.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14585..15154
                     /gene="rnmV"
                     /locus_tag="ACG8V1_001448"
     CDS             14585..15154
                     /gene="rnmV"
                     /locus_tag="ACG8V1_001448"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0329546.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15147..16031
                     /gene="rsmA"
                     /locus_tag="ACG8V1_001449"
     CDS             15147..16031
                     /gene="rsmA"
                     /locus_tag="ACG8V1_001449"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0329547.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16482..17111
                     /locus_tag="ACG8V1_001450"
     CDS             16482..17111
                     /locus_tag="ACG8V1_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329548.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17235..18344)
                     /locus_tag="ACG8V1_001451"
     CDS             complement(17235..18344)
                     /locus_tag="ACG8V1_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329549.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18553..18975
                     /gene="mgsA"
                     /locus_tag="ACG8V1_001452"
     CDS             18553..18975
                     /gene="mgsA"
                     /locus_tag="ACG8V1_001452"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0329550.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19162..19260
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19298..19834
                     /locus_tag="ACG8V1_001453"
     CDS             19298..19834
                     /locus_tag="ACG8V1_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0329551.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19874..21637)
                     /locus_tag="ACG8V1_001454"
     CDS             complement(19874..21637)
                     /locus_tag="ACG8V1_001454"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329552.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21640..23355)
                     /locus_tag="ACG8V1_001455"
     CDS             complement(21640..23355)
                     /locus_tag="ACG8V1_001455"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329553.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7580 a         4302 c         4776 g         6849 t
ORIGIN      
        1 ctgaaaaatt ccttgaaacc tcacgaaaaa atcattattt tatgaaaaag acaaaaaaaa
       61 gtgttgattt tttaataatt attcattaaa ctcttaaaca acttcagaaa atattaatta
      121 ttctgaaaat tccaataaag gggtacgtaa aatgaaaaag aaatttacat ttggtttggt
      181 tgcggtatgc ggccttgtac ttgctggatg ttatggcggc agttcagcca caagcagtag
      241 ttcagcaaaa agcggttcag cagatggagg aggcgtattt aacttagtcg tgcctcaaga
      301 aatgcctaca gctgacttat cagttgcaac agatacaatc agttttacag cattgaataa
      361 cgtttatgaa ggaatttacc gtcttgatga agacagcaaa ccacagccgg caggagctag
      421 cgagttggca gaagtcagtg aagacggatt aacttacaaa atcaaattac gagaagacgc
      481 aaaatggtca aatggtgatc ctgttacagc agcggattac gtttatggct ggcaacgaac
      541 agtagaccca gctacagctt ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga
      601 agacattaca gctggaaaga aagataaatc agaactaggg atcaaagcag ttggtgacta
      661 tgaattagaa atcaaattga cgaaacaaac accatatttc caatatctgc ttgctttccc
      721 atcatttttc ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct atgcatctgc
      781 aagtgataaa gctgtttata atggaccatt cacattagaa gattttgatg gaccaggaac
      841 agatacagaa tggacttata agaaaaatga tcattactgg gacaaagata cagtaaaact
      901 ttctgaaatc aaagtcagtg tagtaaaaga atcttctact gcattgaact tattcaaaga
      961 tggccaagca gatgacgtga tcttgtctgg tgagttagca cagcaaaacg caaacgatcc
     1021 agcttatact tctgttaaag aagcgcggac aagctatatc gaattcaatc aacgtgaaaa
     1081 gaattcacca ttcaacaatg tgaacctaag aaaagctatt tcttattcta tcgatcgtga
     1141 agctttagta aaacaagtac taggtgacgg atcagttgtc tctacaggat tgatcccatc
     1201 agatatggta aaaagtccaa caacgaacga agactttgca aaagaagctg gaaaattagt
     1261 ttcttatgac aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt
     1321 tgattcttta gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga
     1381 atatgttcaa aactcaatcc aagaaaactt ggatggcgta acagtcaaac caacacctgt
     1441 tccattctct gtacgtcttg accgctcaac ttcgggtgat tttgacgcag tactaggcgg
     1501 atgggctgct gactatgcag acccaagcag ctttactgat ttgtttgtca caggtaattc
     1561 atacaaccgt ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag cagcttcatc
     1621 aacagacgct ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa tcaagatcat
     1681 ctctgatgat atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga
     1741 aaaaatcaaa ggtatcgttc accatgcggc aggcgcttca tgggattaca aatggacata
     1801 cgttgaagaa taatcgttaa taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc
     1861 gcaacctcgt tctcatattc acttatccta acaaagaaaa aagcatgaaa atagcagaaa
     1921 gagcaattag tattccggca atgatcagcc aaaagctgta cataccgtag ctggcagacg
     1981 agagcgagga aaattctggt tttttctttc ggttccttgt tcgtgcaaga tggaccgatc
     2041 gctgaaaatg atcgaagaaa cccgaagaaa agacccataa aaaaccgccg ataatcagaa
     2101 acggcaatga ccataagaat agatcattag ataattgaat gagtgtaaag gaatggttga
     2161 tgagattttt tattaaaata accagaatca gtagaaaagc tactgcgtag gggatagatt
     2221 tttttttcat ttgattgctc ctttattttt ggttcattat ctaataatgt agcgaaacat
     2281 ctgtcagaag tcaaaagcta gggggaaaca ttagtgaata gttatatcaa atatgtatta
     2341 aaacgtgtct ttttcatgat catcacgctt tggttaattg caacaatcac ctttttcttg
     2401 atgcaattgc tgccgggcac gccatatacg aatcaggaac gtttgagtcc tgaaacaatc
     2461 gagatgctaa ataaacaagt cggtttggat aaaccagtta tcgtacaata tgggatttat
     2521 ctttctaatt tgcttcaagg agattttgga atttccttcc aatttaaaaa ccagccagtc
     2581 gcacatttgt tagctggaag aatcggacca tccttgcagt tagggttaca ggcaattatc
     2641 tttggaacag tcctagggac gattttagga acgatctctg ccatgaagca aaatacatgg
     2701 gcagatacat cttctacact cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt
     2761 gctgttttat tacaatatat ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg
     2821 gatggctttg tgtacacaat cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac
     2881 tctgctcggt ttatccgtac ggaaatggtg gaagtactgc atagtgatta tgtggaatta
     2941 gcaaaagcaa aaggattgag ccgttgggaa atcgccttta aacatggtct tcgaaatagt
     3001 ttgattccat tgatgacttt attaggaccg ttagctgtag cattgatgac cggatcactg
     3061 gtggtagaaa atatctttgc tattcctggt atcggggaac aattcgttaa gtcaatcaca
     3121 acaaatgatt atcctacgat catggctgta acgatccttt actcatttat gctgattttt
     3181 gttattttga tcgtggatct attgtacggt ctagttgatc caagaattcg tgtttcagaa
     3241 gggagccgag gataaatgga aatgatacca aagcgttatg aaacagttgc agatatccct
     3301 gcggatgaat ttcttccatt acaaaaaaat accgaagaag aacgtgaaca gattactgca
     3361 ccttctttga attttatcca agattcatgg cgccgtttaa agaaaaacaa agctgccgtt
     3421 atttcaatgg ttttactatt ggtcatcatt ttgatctctg tgatcacaat ctttgtttct
     3481 cctcatgatc caactgccca aaacgtggct tatatcaatt tgcctcctcg tattccggga
     3541 atcaatatcg atggattgaa tggaaaagca atggttggtg gtgaacttgt tgataaatat
     3601 gctcaagcaa acgtcccttc aaacgtaaat ttctttttag gaacagacgg tttgggacgt
     3661 gatgtattaa gccgcttatt tatgggaatg agaatctcgt tgctgatcgc atttattgct
     3721 gcattactag atgtaacgat tggtgtagct tacgggttgg tttctggttt gttaggcgga
     3781 cgtgtcgata acgtgatgca acgttttttg gaaatcttat caggaattcc aaacctcgtt
     3841 gtcatgattt tgatgttggt cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg
     3901 gccatcacaa actggatacc aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa
     3961 gatcaagaat atgttttagc tggattgaca ttaggtgaat caagaatgaa aattgcattc
     4021 aaacatattt tgccgaatat ctctagtgtc attattattc aaatgatgtt tagtattcca
     4081 tctgctattt tcttcgaagc ctttttaagc tttatcggtt taggtcttac gcctccgtca
     4141 gcttcattag gaacgatgtt gagcgaaggg tacaagacat tcctgtatct gccttatcta
     4201 ttatggattc cggcagtaac tttatcggtt atcatgattg gctttaactt acttgctgat
     4261 ggattgcgtg atgcctttga tcctaaaatg aaagagtgaa gacgatgacg aaaattttag
     4321 aagtgaaaga cttagaaatc tcttttgata cgtatgctgg aaaggtacga gcgattcgcg
     4381 gtgtcaattt tcatttgaat aaaggagaaa cattggcgat cgttggggaa tctggaagtg
     4441 gaaaatcagt gacgaccaga agcatcatgc gcttgctgtc aagcaacgcc aatatagatg
     4501 ctggtcaaat cctctttaaa ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa
     4561 aaatccgtgg taaagaaata gcgatgattt tccaagatcc aatgacctct ttagatccga
     4621 cgatgcccat tgggaagcaa gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa
     4681 aagagggaca aaaagctgca ttagaattgc ttaaattagt tggaataccc gaagctgaga
     4741 aacgtatcaa cagttatccg catcagttct caggaggtca gcgtcaacgg atcgtgatcg
     4801 cgattgcact ggtatgttat ccggaaatac tgattgctga tgaacctaca acagcgcttg
     4861 atgtgactat tcaagcacag attttggagc tgctgaaaga aattcagaca aaaatcgata
     4921 catcaattat ctttatcact catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg
     4981 ctgtcatgta tggaggaaga atcgttgaag ttggaacatc ggaagaaatt ttctacaacc
     5041 cgcaacatcc ctatacttgg ggactgcttg gttcaatgcc gacattagat agtgcgaacg
     5101 atcgattgta tgctattcct ggttctccac cggatttatt gaacccgcca aaaggagacg
     5161 cattctatcc aagaaatgaa ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt
     5221 ttgaactatc aaaaacacac aaggcagcaa cttggctact tgctccgcag gcacccaaag
     5281 tgacaccgcc agaagaaatc caacgtcgct gggctatctt taaagagaaa caaaaacata
     5341 cttacggtca aggaggaatt gctgatgcca aataaactac ttgaagttaa aggattgaaa
     5401 cagtatttca atgtaggaaa aaagaatgaa gtacatgctg taaatgacat cagctttcac
     5461 atttatgaag gagaaacatt cggtttagtt ggagaatctg gaagcggaaa atccactact
     5521 ggccgtacga tcattcgctt gaatgaacct actgatggtg agatattatt cgacggtcaa
     5581 gatataacga agatcaaaga taaaaaaggt ttgacgaaat tccggcatga tgttcagatg
     5641 atctttcaag atccatacgc ttcgttgaat cctcgtatga aagtcagaga tatcattgcc
     5701 gaaggaattg atgttaatgg actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat
     5761 gatctattga gaactgttgg attgaatcca agccacggta ctcgttatcc tcatgaattt
     5821 tcaggtgggc aacgccagcg tattggtatt gcacgagcat tggcagtaaa accgcgtttt
     5881 atcatttgtg atgaaccaat ttctgcacta gatgtgtcga tccaagccca agtggtcaat
     5941 ttgttgcaag atctccaaaa agaacatcaa ttgacctatc tatttattgc acatgatttg
     6001 tcgatggtta aacatattag tgaccgaatc ggtgtaatgc ataacggctt gttattagag
     6061 atgggaacaa gtgatgaaat ctataatcac ggtgttcatc cttatacaga aagtttgtta
     6121 tctgctattc ctctaccaga tccagaccat gagagaaaac gcagaagaat caaatatcaa
     6181 cctgaaccag atgacggaca agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc
     6241 tatgcaactg agcaggaagt cccatattac gcaaaaaaac taaaacgaca aaaggaatct
     6301 cttctggttg ctaactagga gggacataga tgctaaaagc ttttaaattc cgtatgtatc
     6361 cgacagaaga acaaaaacag caattgattc gtacttttgg atgtgctcga tttacctata
     6421 accatttgct gaaaaagcgg cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc
     6481 tcacacccgc tacattaaaa aaagaatacc cattcttaaa agaagtagac agtcttgctt
     6541 tagcaaatgc acagcttaat ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca
     6601 gcttcccaaa gcttaaaacg aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa
     6661 cccgtacgat ctatctggaa aatggctact taaagttacc aaaacaaaaa gaactaatca
     6721 agataaatca gcatcggcca gttgaaggat cgattcgctc cgctacgatt tctgctcgat
     6781 ataatgaaga attttatgtc gcactacttt gtgatgtttc gccagtaaaa aaagaatctt
     6841 tagctaaatg gatcgggatc gcctatcatc cgaagacatt gatccaaaca tctcgacctc
     6901 tagaagtaac tttaccaaaa tttcaccaga cagaagaaaa gctgcaacat gcacagcgga
     6961 aattgaatgt gaaagtccgc tccgctcacc atagaaagat caggttagat caggcaagca
     7021 attatcagaa acaaaagaga aaagtaatgg atctttatct gaaacaaaag aaccagcgag
     7081 aagattatct tgaacaatta tctgggaaat tggtcaaaca atatgactac ttatttgtag
     7141 aatcttttcc aaaagaagag gcacatgctg atttttctat tcacgactgg cataaattaa
     7201 taacaaaatt gcgttataag tcccaatggt ataataaaaa atttcttttg atcaataccg
     7261 acggagcaga agaaagcaat tccgtaagaa agagccaagt agtagaaaaa atgggcagac
     7321 attcggttat caagggataa aaaatgaatg aaactgatag gagcgttagg gttagccttg
     7381 ttaataaaaa gtcttaatga cttttgtttc taggaatttt tcttttgaag aaaagctcac
     7441 taattatgac aaaccaataa aacaatagca gaaaaagttc gtacgagctt tttctgctat
     7501 tgttaatttt ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa
     7561 ctcacaaaga gttgtttttg cgttttttct tgaatgatta ggtctgaatc ttttgctcac
     7621 aaccttattc atggtgcccc ggcttgagct gatagatttt tgaaggacgg ccgattccta
     7681 caggacgttc accgatctca tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat
     7741 cgatcgtttt ataatcgatc cctaagaatt tagcaaatac ttttcgagct tccgtaatcg
     7801 tgaaatcttt acctaaaact tgtagaactt gcggctcgtg ttccatttta ttgactacac
     7861 ggttgaatgc tttcaagatg atttgactat ggtcaaaagc caaagtatct ttgccagaag
     7921 actctccagt gactaaatca agcgagatag ccacttctcc gctagataaa tggatcttgt
     7981 tactatgacg ttctagtgtg aaccagcgta cttccttggc atcatctccg gcgattagcg
     8041 gttcttctcc aataaaggct aagtagctga ctgtgacgac ccagcctcgc ggatctcgat
     8101 taggcgtact gaatgtatgc agttgctcaa tattttcttt tgaaataacc acaccagttt
     8161 cttcctttgt ttctcttaag acgctttctc cagttgactc atccttttgg acaaagccac
     8221 caggcaatgc ccatgaattt cgataaggat gcccttttct ttggatcaac aaaactttca
     8281 gctgatcttc ttctttgtta tagcacatca gtacgatatc gacagtcaaa gagggcttgt
     8341 catattctgg acgttcctgt ttttgatacc agttaagaaa ttcttcttca cttgctttat
     8401 gttcataata taatttttct tcttctttag aagcaaaacg gttcatctga ccacctccat
     8461 tgacagtaaa agtacttttt accttattgt agcagactct gaaaaaaaac caaggattta
     8521 cttgaaaaat ctaggttatc atttaaatat gaaaaataaa tggaagtatg agattgaata
     8581 aaaagaaaag cagataaaac ggcaatactt tataccgctc ttcacgaatg gcaaagaagc
     8641 cacaaataac gatatatacg tcaatgataa aacgcaaaat ccaagctgct gtcataaaac
     8701 tgttcaaatg cccatggagg ttaaaataac catacatacg aatataaaaa tagctgaata
     8761 ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca
     8821 tgattgttcc agtcccttac taagatagat agtctttatt ataccacgtg tgaggggaga
     8881 aataaaaagc cgttttttag tagatatgaa acaaaacaaa aacccgcatt tattcaacct
     8941 attcatcctt ttaaaggata gattgaacgt agatagaagt cgtttatcca gtctttttca
     9001 ttttgaaaaa aagctaacag catgcttttt ttatggtata ctagcaagga aacgaattcg
     9061 catcatgcga taaaggagga agagccccgt ggcagaaaag gaaacttttt atatcactac
     9121 gccaatctat tatccaagcg gcaaacttca tatcggcaac tcgtatacta cgattgcatg
     9181 tgacgcaatt gctcgttata aacggttgat gggctttgat gtgttttatc tcactggagt
     9241 ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga
     9301 atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta
     9361 tgataaattc attcgcacga cagatgatta ccataaagca gctgttcaaa aaatatttga
     9421 tcgtttatta gaacagggag atatttactt aggtgaatat gaaggctggt actctgtttc
     9481 tgacgaagaa ttctttacag aaacacaact tgcagaagtg tacaaagatg acgaaggtaa
     9541 cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag aagaatcata
     9601 cttcttccgc atgagcaaat acgcagaccg tctattggaa tactatgaag aacatcccga
     9661 attcatccag ccagaatctc gtaaaaacga aatgatcaac aactttatca aaccaggatt
     9721 agaggatctt gcagtttcac gaacaacttt tacttgggga gtaccagtca agagtgatcc
     9781 gaaacatgtg gtgtatgtat ggatcgatgc attatccaat tatattactg cactaggata
     9841 tggctccgaa gatgaaagtc tattcgaaaa atactggcct gcagatgttc agatggtcgg
     9901 taaagaaatc gttcgctttc atacgatcta ttggccaatc atgctgatgg cccttgattt
     9961 gcctctacct aaaaaagtat ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc
    10021 taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg gtttagatgc
    10081 actccgttat tatttgcttc gtgctgttcc gtttgggtca gatggtgtct ttacgccaga
    10141 agattttgtt tcccgtgtca attatgatct ggcaaatgat ctagggaact tattgaatcg
    10201 aacgattgct atgatcaaca aatattgtga tggactcgtt cctgactatg cctcacttgt
    10261 cactccattt gatagtgaac tgtctacgac ggctgctaac gttgtcggac gttaccatga
    10321 tgcaatggaa aaaatggaat tcaatacagc attggctgaa atctgggtgc tgatttctcg
    10381 tgcaaacaaa tacattgatg aaacagaacc atgggtatta gcaaaagacg aagaaaagaa
    10441 agctgaattg aatagtgtca tgatccattt agctgaatct ttgcgtatcg cagcgatatt
    10501 acttcagcca atcatgacag aaacgccaga gaagatcttc aatcaactag gattggactc
    10561 tgaaacaatg aatcttgaag gattacactt tggcgaattt ccttcaggaa caaaagtagt
    10621 tgcaaaaggt acaccaatct tcccacgttt agatatggaa gaagaagttg cttttattca
    10681 agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg atccagaaga
    10741 aacagaactt gtttcaacga aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga
    10801 actaaaagta gcagaagtca tcaactgtca aaaagtagaa ggggcagata agttgctgca
    10861 attccgacta gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt
    10921 ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc
    10981 acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag caccagatgg
    11041 ctctttacaa gtaatcgaag caccaaaatc catgccaaat ggctcagaaa tcgcatagaa
    11101 attcaaaggg ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca
    11161 attttcgtaa attccttctt attacttgaa gccaatcata taatacagaa acagagaact
    11221 atccaaaata aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat
    11281 aatactaata ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt
    11341 ttaattgatt catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa
    11401 tgactttatt acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc
    11461 tatcacttta tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa
    11521 aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac
    11581 ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc
    11641 agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga
    11701 tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata
    11761 aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa
    11821 taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat
    11881 aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa
    11941 tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac
    12001 aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt
    12061 aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg
    12121 gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc
    12181 gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg
    12241 agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg
    12301 aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag
    12361 aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct
    12421 tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt
    12481 ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag
    12541 caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca
    12601 gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat
    12661 cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt
    12721 tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg
    12781 gtgcttctgt acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca
    12841 tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa
    12901 aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg
    12961 acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc
    13021 ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg
    13081 tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag
    13141 catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct
    13201 cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg
    13261 ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg
    13321 ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt
    13381 ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt
    13441 ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac
    13501 cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga
    13561 cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc
    13621 cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca
    13681 gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt
    13741 tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga
    13801 aaaggagaga taaacatgat atttgattct catacccatt taaatgctga acagttcaat
    13861 gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa aatggcggtc
    13921 gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga ctatccaaat
    13981 atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa agaaatcgaa
    14041 aataaattac aagaacaatt gactttacca aaggttgtag cattagggga aattggcttg
    14101 gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg cagacagatt
    14161 gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc acttgaagac
    14221 acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat gcatagtttc
    14281 agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat ttcatttagc
    14341 ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa tgtaccaatc
    14401 gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt tagaggaaag
    14461 cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact tcggaatctt
    14521 ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt tggtctaagc
    14581 gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag acgatacaag
    14641 aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg caatcaatga
    14701 agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg tttttaccga
    14761 tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc ctcaggcaaa
    14821 acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc ttggtgtcga
    14881 gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc cagacatttc
    14941 ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt taatagctgg
    15001 ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa
    15061 cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag aactcgccca
    15121 agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg acaccctcta
    15181 gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta ggacaaaatt
    15241 ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata
    15301 cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta gccaagcatg
    15361 caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg gctgatacga
    15421 tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc gatttgtcaa
    15481 cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg aatttacctt
    15541 actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt gtggatgaac
    15601 tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca ggaacgaaag
    15661 cttatggttc attatcgatt gctgttcaat attatatgga agccagttta gcattcatcg
    15721 taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg aaactgacaa
    15781 gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga ttgacaaaag
    15841 cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg tatggcaaag
    15901 atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc gatccaaaac
    15961 gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa
    16021 atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta
    16081 ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttattaccg
    16141 cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta
    16201 agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt
    16261 acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt
    16321 cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt
    16381 ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa
    16441 gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc
    16501 gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc
    16561 ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg
    16621 cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct
    16681 ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga
    16741 ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt
    16801 ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag
    16861 aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc
    16921 aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag
    16981 ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg
    17041 tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa
    17101 cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc
    17161 ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc
    17221 taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg
    17281 cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca
    17341 tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca
    17401 agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct
    17461 acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa
    17521 cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca
    17581 tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata
    17641 aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt
    17701 tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga
    17761 tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca
    17821 cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact
    17881 tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa
    17941 gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca
    18001 cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca
    18061 aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct
    18121 ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct
    18181 tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa
    18241 acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca
    18301 ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt
    18361 tcttttttag ttttattaac tatttaaagt ataaatgaaa gcgttaacta acttcaatgt
    18421 catgttgaac aagaattttt tctttttttt gtcaatgtgc tagttttaag ctttaataaa
    18481 tatccataag aaataaaaac tgatacactg gagtaaataa gtatgttaac aatatcaaaa
    18541 ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg
    18601 aaattagcaa cagcgtataa aacaatattg gaaaaacatg aactttttgc taccgggacg
    18661 actgggcaga ggatcacgga agcaaccggc ttgccgatcc atcggtttaa atccggaccg
    18721 ttaggcgggg atcagcaaat tggagccatg atttcggagg atcagttaga tttggtgatt
    18781 tttcttcgag acccgctggc tgcacagccc catgaaccag atgtaacagc acttatccgt
    18841 ttatctgatg tctatgagat tccgttagct acgaatatcg gaacagctga ggtgctacta
    18901 agaggactgg aagccggctt cctagatttt cgaagaatcg tccatgaaat agacaagcat
    18961 ccactttcat tttgatggat aagcgtatgg aaaaataacg tttaatgttc gcctatatgt
    19021 agctataaaa gctaaacaaa ctgataatat gcgatataat acgaacgtat tggataatat
    19081 aaacaataat attagttttt cgtatttgca gtaaatgtga aaatatgcta tagtttctgt
    19141 gggaaaagat cccaaaaata atacagagta gaaaataaag cgttaagtgc taagaggatg
    19201 ggatgttgcc tcttggacga aggacgtaat gttcgcggtt ttattttcgc attcgctgca
    19261 tgatacgatg tagcagctct taaaggagat gctaaaaatg aagaaacacc ggttagacgc
    19321 acgtatgatt gctattatgg gcttattgat tgcattgatg gtcactttgt cccgactggt
    19381 agcaatcgag accccattta ttaaaatcag tgtcacattt attccacaag tgatcatggg
    19441 cattcttttt ggtccttttt ggtcagggat aggcgcagtt ttagctgatt tagtaggaat
    19501 ggccctcttc tcgaaatctg cattctttat tggatttaca ttgaatgcat ttattgaagg
    19561 agcaatctac ggattctttt tctatcgaaa agagatcact tggaaaaatg ccattctggc
    19621 tacgcttagt gtcacgctga tcatcaactt atttttgaca ccattatggc ttgcgttgat
    19681 gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat
    19741 atggttaccg attcaatcaa ttgccattta ttacgtagga cgtagtattc cttacaaaaa
    19801 aattttaaga agtttagcga tccacgctaa ataaaaaaca agcagtcttc ccatcccaga
    19861 agactgcttg tttttattgt gctgtgaatt gtgcttgata gagccgtcgg taataaccat
    19921 ctttttgaag cagctcatca tgggtgccca tttcgacaat agctccctga tccatcacta
    19981 atatctggtc cgcatttttg attgtggaca ggcgatgggc aatcacaaaa ctggtttttc
    20041 cttccatcat tgctaagaag gcttgttgga tttttttctc tgtcaatgta tcgactgagc
    20101 tggtagcttc atctaagatc aacatagggg gattactgat catcgttcga gcaatcgtca
    20161 acagttggcg ttgtccatct gatatcttta acccttgacc gccaatctta gtatctaatt
    20221 tttccggcaa acgttctaca aattcataca tataagaggc ttttagggca tcattgattt
    20281 ctgcgtcact tgcttccgga ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata
    20341 gccaagtatc ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt tggatagagc
    20401 ggatgtcatg gtcatcgact gtaattgttc cttttgttgt gtcataaaaa cgcatcagca
    20461 aattaacaag tgtggatttt ccggcccctg tttttcctac tatagcaatg gtttgacctg
    20521 gataagcagt aaaattgaaa tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa
    20581 catgatcaaa agagactttt cctttgatat ttttgggttc caaggcatag gcatctggca
    20641 aatcaggctt ttcttctggc tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag
    20701 cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa aactgacttg
    20761 agtagatcgt aaagctggaa atcacaccaa cagtgattga cgaaccactc ttcaatgcca
    20821 ataggccgcc aactaaacca atcgataaat aagcaaggtg atctacaaaa cgagaagacg
    20881 ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt ttcatataga tcagcattta
    20941 gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa agctttcacg attttctggt
    21001 taccgattcg ttcggtcaca aagcccgaga tatccccaat aatcctttgc tgtctatcga
    21061 aatttgtttg tgaagcacga gcaacgatcc aactcacaag gaaaataatt ggtgtagaac
    21121 aaagaacgac caatgtcaga agtggactta gacgcatcat aaaaataaac gaaagaatga
    21181 tgattgccac tccagaaaat aattgattga aggccgcaga aattgcaatg gaaatattgt
    21241 ccatatcatt tgtaaatcga ctgacgatat ttccgtggga gttttgatca taataacgaa
    21301 gtggcaatcg attcagatga gcaaatgcat cttttcttaa ttttgcaacg gataagtaag
    21361 ctaaccgatt gcttaacact tggatgagcc attgactgac tacgttgatt aaaacgatac
    21421 caccgaatag aataagtaaa tggaacagtc gattgaagcg gacctgtcct tctccaatca
    21481 tttgatcgat actttgacca atttgtaacg tcatcacaac tgttgcaatc ccattgatga
    21541 ttcctagtat cacagctagc caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc
    21601 ggcggagcgt ctgcaaggat aattttttta ctttcataac tattcttcct cctgtgatgc
    21661 gacgatttcc tgatattctg ctgattgagc aagtaattct tcatgggtac cctgagcagc
    21721 aatcttacct tgatccatca ctaagatatg atgtgcttct tgaatagagc ggacacgttg
    21781 ggagataata atcactgtac tgccaagata agtctgtagc gccttacgca aatccaagtc
    21841 tgtttgataa tccaacgcac ttaaagagtc atctaaaatc agtaatgctg gcttggcaat
    21901 caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt tcccattttc
    21961 catgactttt gtatctaaac catttggaag ctgacggaca aattcttctg cctgagcgat
    22021 tttcagagct tcccaacatt ctttttctgt ggcgtctttc ttaccccatt gaaggttttc
    22081 acgaatagtc ccggtaaata gtacagaagt ttggggaaca agagcaattt gctgacggag
    22141 atgatccagc tgccaagaac gaacatccat tccgtccatt ataactgtac cggaactgac
    22201 atcatagaaa cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat
    22261 gattccaagg actgtatttc gcttcagctg aaatgtaata ttttggagag aaagaccaga
    22321 atcaggagta taacggaagt caacatggtc aaaagcgata attgcttcag aagaaaaatc
    22381 gggagtagta ctgacctcag cttcaagaat cgaattttct gtgtctagca cctctttcac
    22441 ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag
    22501 tgccaaaagc atttgattca tataattgat caaagcaaga acttgtcctt gttgcaagct
    22561 accaatattt actttgatcc cgccaactgt cagcaaacag atgatcccga cattcatgat
    22621 caaggttgta gcaggtgata atagggcaga gatattggaa acacgtaaat aattgttcgc
    22681 tagatcattt gtgcttttat cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg
    22741 gatgacacga acaccgctaa gattctgact aacgtgttca ttgagctgat ctagtttttc
    22801 ttgcactttt tggtagagag gaacggtttt tttgatgata aaaaataaaa tgattgagaa
    22861 aataggcaat aatgctaaga aaaacagacc tacttcccag tcgatcacaa aagccatgac
    22921 cactgacccg atacttaaaa acggtgctct gatcaataaa cgaatgacca ttgctaaagc
    22981 tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg
    23041 attcaactcg ttgtgagaga aagtattgat ttttttgatt agctgattac gtaactctgt
    23101 accaaatcct tgtgaagcta ctgaagcata gtattggcaa atgatagcac aaatcagacc
    23161 aatcacagac atagcgacca tccataagcc catttcaatg atttttcctc ggtcattcat
    23221 ttttagtcca ttatcgatca aggaagccat cattaaaggc aggacaagtt cgaagacagc
    23281 ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta cgatagtctt ttgcataacg
    23341 taataactga atcatatgat tcctcctatt atttatttgt ccacaattga aaaaacaagt
    23401 actaccacat tgtagcttag gaaatttaga atgaaaagag aacaagcctt ttcataaatg
    23461 aaagttttat tatgaagaag ctttagaaat tgaatagact atttttt
//
LOCUS       DAZKMK010000039        23412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000039 DAZKMK010000000
VERSION     DAZKMK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.039"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1074)
                     /locus_tag="ACG8V1_001456"
     CDS             complement(31..1074)
                     /locus_tag="ACG8V1_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0329554.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1235..1918)
                     /locus_tag="ACG8V1_001457"
     CDS             complement(1235..1918)
                     /locus_tag="ACG8V1_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0329555.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2113..3042
                     /locus_tag="ACG8V1_001458"
     CDS             2113..3042
                     /locus_tag="ACG8V1_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0329556.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3103..3894
                     /gene="phnC"
                     /locus_tag="ACG8V1_001459"
     CDS             3103..3894
                     /gene="phnC"
                     /locus_tag="ACG8V1_001459"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329557.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3866..4645
                     /gene="phnE"
                     /locus_tag="ACG8V1_001460"
     CDS             3866..4645
                     /gene="phnE"
                     /locus_tag="ACG8V1_001460"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0329558.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4642..5424
                     /gene="phnE"
                     /locus_tag="ACG8V1_001461"
     CDS             4642..5424
                     /gene="phnE"
                     /locus_tag="ACG8V1_001461"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0329559.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5427..6230
                     /locus_tag="ACG8V1_001462"
     CDS             5427..6230
                     /locus_tag="ACG8V1_001462"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0329560.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6314..7861
                     /locus_tag="ACG8V1_001463"
     CDS             6314..7861
                     /locus_tag="ACG8V1_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0329561.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8158..8355)
                     /locus_tag="ACG8V1_001464"
     CDS             complement(8158..8355)
                     /locus_tag="ACG8V1_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329562.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8431..8631)
                     /locus_tag="ACG8V1_001465"
     CDS             complement(8431..8631)
                     /locus_tag="ACG8V1_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0329563.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8751..9938)
                     /locus_tag="ACG8V1_001466"
                     /pseudo
     CDS             complement(8751..9938)
                     /locus_tag="ACG8V1_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9983..11109)
                     /locus_tag="ACG8V1_001467"
     CDS             complement(join(9983..10881,10881..11109))
                     /locus_tag="ACG8V1_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0329564.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11513..12295)
                     /locus_tag="ACG8V1_001468"
     CDS             complement(11513..12295)
                     /locus_tag="ACG8V1_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329565.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12360..13196)
                     /locus_tag="ACG8V1_001469"
     CDS             complement(12360..13196)
                     /locus_tag="ACG8V1_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0329566.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13214..14623)
                     /locus_tag="ACG8V1_001470"
     CDS             complement(13214..14623)
                     /locus_tag="ACG8V1_001470"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0329567.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(14637..16460)
                     /locus_tag="ACG8V1_001471"
     CDS             complement(14637..16460)
                     /locus_tag="ACG8V1_001471"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0329568.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(17303..17629)
                     /locus_tag="ACG8V1_001472"
     CDS             complement(17303..17629)
                     /locus_tag="ACG8V1_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329569.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(17675..18037)
                     /locus_tag="ACG8V1_001473"
     CDS             complement(17675..18037)
                     /locus_tag="ACG8V1_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0329570.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18074..18288)
                     /locus_tag="ACG8V1_001474"
                     /pseudo
     CDS             complement(18074..18288)
                     /locus_tag="ACG8V1_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            18416..18751
                     /locus_tag="ACG8V1_001475"
     CDS             18416..18751
                     /locus_tag="ACG8V1_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0329571.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(18827..20824)
                     /locus_tag="ACG8V1_001476"
     CDS             complement(18827..20824)
                     /locus_tag="ACG8V1_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329572.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(20922..21197)
                     /locus_tag="ACG8V1_001477"
     CDS             complement(20922..21197)
                     /locus_tag="ACG8V1_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329573.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            21429..22163
                     /locus_tag="ACG8V1_001478"
     CDS             21429..22163
                     /locus_tag="ACG8V1_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0329574.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            22202..23344
                     /locus_tag="ACG8V1_001479"
     CDS             22202..23344
                     /locus_tag="ACG8V1_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0329575.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         7223 a         4622 c         4094 g         7473 t
ORIGIN      
        1 tgtagacatc atccataaaa tggactttgt ctacagtctg aaatcaccac tgatgatttt
       61 ctcttttgcg tctgaaactg cctgataggc ttcctctgat aattgtcctt ttgtcagatc
      121 taccccttct tctttcaaac caaaggtcaa atgcttgccg ccaggaaatt tatcgttatg
      181 agaagtttct gcaaattctt tgacttctgc accgacattt ttctttgtag aagtcaacgt
      241 gaaattccct gtctcgccgt tctccatttt ataatcccct tcatccgctt gatcgcgatc
      301 tacaccaatc acccatactt ttctgctata gtcgttttca ttgcgggctt ttgcttcttg
      361 gaacacccca gctccagtac ttcctgccgc ctgataaaga atatcgactc cctctttgta
      421 catagaagca gcaatagctt ttcctttatc tagcgaatca taggatgcag catattgtac
      481 gttaacagta atttccttgt tcagtgctgt ggcccctgcc ttaacgccag cttcaaatcc
      541 gctttggaaa cgttggatca cgggcccttc cattccacca ataaagccta cttgatcact
      601 ttttgtcgta tatgcggcag ctagtcctgc taaatacgca ctttcttgat ctttaaaggt
      661 tgctgatgag atattctttt tcccagtaat ctcaccatct accaaggcaa atttcaactt
      721 tggattatct ttagcagcat tttccagttc ctctgcttgc aagtagctca agctaataat
      781 ggtggtaaag ccgtttctga ttgcctgatc gatattggta ctgttatcct ctggattgtt
      841 cgtttctata tacgtaaaat ctttttttgc tgtcagcttt ttctcttttc cccaagcctc
      901 taatccttcc cagccagctt gattgaagga gcggtcatca accccgccaa atccaagaac
      961 caaagcgatt ttacttttct ctttttcact ttgcttctcc ccctgagact gactacagcc
     1021 gactaacgtt actaatgaca ccaaaaacaa caatgaaaca agtattttct tcaaaatttt
     1081 tctccccttt ttatgtgtct tgacacattg aacttttaaa agtatagcat accaatatat
     1141 agaacactac tcttcaacga agattttccg tttcccaatt tctccactat caaaaaacca
     1201 gcaatcactt ctgactgctg tttttttata gctgttattc tgcttttact ggactactta
     1261 tattctcagc taagtcctct aaagcttcga tatcatgaat caagtaataa ttctttttct
     1321 tttcgataat attctcctca cgaaaattcg tgattgttcg taaaagatgt cgataactga
     1381 catttaagat tgaggcgcag gtggttaacc gaaatgagaa acgttcgttt tcgtgatggg
     1441 tcaaaataaa ctttgctaaa cgagtttcaa caggttctgt cagtgagttc gccaaattta
     1501 tcccggaatt taaacgataa cttagtaact ggcaaatatt ctgcaaaaag cgaacatctt
     1561 tttgaagctg tgttcgatat tgatttagcg gaatgctgat acagatgcac ttagtcactg
     1621 ctttaacgct gcttttcggc gataattccc acaatgaaga ggcttcgccg agtggtgtag
     1681 cgggctttgc gtaatcgatg caaatatttt gtgtatcaga agaatacgag tacaccatta
     1741 ccgtcccatc cactaaaaaa tacaaataat ctgaggtttt tcccgtttga atgaggtgct
     1801 cttccttttc aaaaaaaagt aaagaagcca ttgccaatat atctgaatcc atataatcct
     1861 gcaggttgtg cttctcaacg taagcagtta attgcttcgt atcatggatt tttttcatga
     1921 aatcgtcacc tctaagaagt ttttctttat tatagcttag attgtttttc ggctcaatac
     1981 gaataattat ttataattgt ttgtaaattg tatgtaaaag ttgtgtaaaa taggacgtgc
     2041 gtcacttcat tgattttgat tccctgctat gatttagacg tattagaaat tatatccaac
     2101 aggaggagaa aaatgcagtt caaacagtta gcaaaagggt tcatcggagt catgttattg
     2161 accactttgg cggcctgcgg ttctgggaat acaagtagca gctcagaaaa agagagcaaa
     2221 gaaaaattag ttgttcaatt cgttccaaca aacaatgatg gtagtatgga ggcaaaagca
     2281 aaaccatttg ctaagtattt atcgaaaaag ttagatcgtg aggtagaagt gacgttagct
     2341 actgattatt caacgatcgt cgaagcgatg tcttctggtc aagtcgatgt cggaattatg
     2401 ccgcctgcag cctatgttca agcaaaggat gctggagctg cagatgcaat cctgacttct
     2461 cagctcggcg attatgatca agaaactggc ttgccattaa aggacaaact tacgaacacc
     2521 tttaaaggag aagtattggt tcgtgccgat agcggattga ataagttaac tgatttaaaa
     2581 ggaaaaaata tcgcaacctt aagtcctaac tctgctagcg gctacatcta tcctgtcgct
     2641 gaattgaaag atgctgggat tgatcctact gcagatacaa agatgaccac tgtgaatgat
     2701 attccgagtg agattaccgc agtgttgaac aagcaaatgg atgcggcgtt tgtttttgaa
     2761 ggcgcgcgaa atgtttttgc ttcgaatttc ccagataatg atttatttaa ggatttaaaa
     2821 gtcctgtacc tgacaaaagg cgatattccg aatgacgcaa tcgctgtaca gcctgacatg
     2881 tcaaaagatt taaaagcgaa aattaaagag accttcttaa atatgaagga tgataaagat
     2941 ggcaaagaag caatggcttt gtgggggcat cagggctatg aagaagctgc tgattctgcc
     3001 tatgacacga tcagagacta cacgaaaaaa gcagcagaat aaaacatcag taagtgtcag
     3061 caagcttttt tttgcttaca cttaaaaaaa tggagaagga taatgattat ttttgacaat
     3121 gtttcgaagg tctatcccaa tggcgttgtt ggcttgaagg atattgactt aacaattgaa
     3181 gatggagaat ttgtttccat catagggttg agtggtgctg gaaagagcac tctgctgcgc
     3241 agtatcaatc gcttggcaga aaccaccaaa ggagatattc gcattaatgg aacctctatt
     3301 acaaaagcga ataaaaaaga gctgcgacag attcgccgcc agattggtct aatctcgcaa
     3361 agcttcaatc tggtaaagcg tagtacggtt cagaaaaatg tgctatctgg acgtttgggc
     3421 tattactcca cttggaaaag tattttaggt ctttttacga aggaagacta ccagcagaca
     3481 aaggaagcac tggaaaaagt tggtttgtct gataaattgc agacacgcag tgatgagctc
     3541 agtggtgggc aacaacagcg agtatccatt gcgcgagcac tcgtgcagca agcccccatt
     3601 attttagcgg atgaacccgt tgcatcatta gatccaatca cgactcaaaa ggttatgatg
     3661 gacctaaaaa atatcaatca aacgatggat aaaacagtca ttgtcaatct gcattcagtc
     3721 cctctggctc gtgagttttc tacgcgtgtg ctcgcgttaa aagctgctca ggttgtcttt
     3781 gatggtcgtc cagacgactt aaccgatgaa cgcttagagc aaatttatgg caatgcaatc
     3841 tttgaagaaa aggcaggcga aatcgatgag cttgaaagaa accgttcact ttaaatggta
     3901 taaacacctg cttattcttc tgttcttaat tgtttgtttt caaggaagtg cctggatcac
     3961 gtcagctgat ttttctcagg tttttcaaaa ctccaatcaa atgactgtct ttttatcacg
     4021 cttcattcat ccagacttta gctatttacc taaattagtc gagcctatgg tgaaaacact
     4081 tcaaatgtct ttgcttggaa cggtcattgg cttaatttta gcgatccctg ttagtttttt
     4141 agcgaccact gtagtgacag aaaaccgtct tctttctacc atctttcgtt ttcttttagg
     4201 tttagtacgg accatcccta ccctgctttt agccgcgctt tttgttgctg tgtttggaat
     4261 tggtgaagcg actggcgtgt tgactatcgc tgtctttact tttggtatgg tgtcacaatt
     4321 aatgtttcaa gcaattgaaa ccattgactt tggcccgatt gaagccgctg cggctgttgg
     4381 agcaaataaa atgcaaatcg ctatttggtc gatcgctcca caagttttga gtcagtttgc
     4441 gagctatgct ttttacgcgt ttgaggtgaa tgttcgtgct tctactgtcc ttggatatgt
     4501 cggagctggt ggtattggcg ttattctaaa ttcctcactt gcgcttttga attatgaacg
     4561 agtttcaatc attattctaa cgatcctagt aactgtcgcg atcgttgata aattaagtga
     4621 acgtgtcagg aggtccctgt tatgatgctt cgtgaaatga acagaagaac ccttttatca
     4681 ttagttgctt tagtgattct cgttattttg tcggccgcta ctctggatta ctctggatta
     4741 agcaccttct ctccttccat gggaatggac gttttgagag ggttaggtca gcctgattgg
     4801 cagtttctat atgatggcag cggcgaggat ttgattagtt tgctattatt aaccattgga
     4861 atcgcctgtt tagggacaat gatcgctacg gtattagcga ttcccattac cttgatcagt
     4921 gcggttaacc tctggcaagc acatccatgg gtaacaaaaa ttggcaagct tatctgtaat
     4981 gttttacgag cttttcctga actagttttt gcaattattt ttgtgaaagt cgtcggacct
     5041 ggcccctttg ctggtgttat ggcgattggc gtccatcaga tcggaatgct tggcaaactt
     5101 tttacagaag aaatggaagc gatggatgag cgacttgttg aagaaatgca tgcggtagga
     5161 gcaaattttt ggcaaacgat tttctttgtt cgcatcccct atttgatgcc gatttattgt
     5221 tctttagcat taaatcattt tgaaatagcg gttcgtagtg cggctacctt aggcttagtt
     5281 ggcgctggcg gaatcggtgc tccattgata ttcgcgattc aaactcggtc ttggagcaaa
     5341 gtgagtatta ttttgattgg cgtagtcgta accgtgttta ttttagatca aataaccgga
     5401 attataagaa agagattgag ataagaatga atatattgca tatatcagat atacactttc
     5461 gcagagagta tgaggcttgt gaagaaggct ataagggaat gctggctacg atgcagaatc
     5521 cccttatccc tctggagcaa tgcattcatc atctcctgca gcaaacaccc ttggaccttg
     5581 tgattatcag cggagattta accgaagatg gcgaaataga cgattatcga tacttgaaga
     5641 cttggctgga aaatgcgcta ggagaaacga cgattcttgt tactctaggc aatcacgata
     5701 tcaaagaaca tttttgggta ggctggtgcg atgaagccgc ctcttctgcc ccgtacaacc
     5761 agatagtcaa atatcctgag tttacagtga tttcctttga taattcaagc tatggatacg
     5821 cagatggtat agtggacgat cagcaatttc aatggctgaa aaaagcctta gagcaagaaa
     5881 aagaccaacc gattttcttg gtcacccatc atcatctact gtctcaccaa agcagtatac
     5941 caaaatggcc tggtaccgaa cgtttcttga agctaatcgc accttacgat attcgctgta
     6001 tcctaaacgg tcatacccat cacacattta ctgatgaaat taatgggatc ccctatttta
     6061 ctgtatcgag tatgtccttt gttggtgaag atgaaggcga tggcttcgta cgctttgaag
     6121 aacgtcatgg gtataacctt tatcatctcg atcaaggaag gattgttcgc cagacaacag
     6181 aaaactttat tccaggcaac attttaaaaa cacttcaaat gaaggattaa ggcattataa
     6241 atcaaaaaaa gagaccgaac agctgaatac gcttcggttt ttctattttg agtcagctaa
     6301 aggagcacgc agaatgaaca ttacgttttt agagacaagt gatatgcatg gctatgtttc
     6361 accaacagac tattccggca gaaccgatct ggccttagga acggctaaag tatcgtcgaa
     6421 actgaaggaa ttacgggcaa aagcagatgg accggtaatt acgatcgaaa atggtgattt
     6481 catccaaggg tctcctttga gttattatct tgctaaagaa tatggttcag ccaaagagct
     6541 tacagacttg attaaccaaa tgagctatga tgttcaagtg ttgggaaatc atgagtttaa
     6601 ctatggtgtg gattatctcg aggaagcgat taagaattat caagcgccta tattaggagc
     6661 caatatggtg aacaaaaatg accaaccata ctttggaaac gcctataaga ttatcgaaaa
     6721 ggtgggtgta aaaattgcta tcttgggatt aacgacacaa tacattcctc actgggaaaa
     6781 gccagaaacc ttagcaggaa ttacgtttaa gagtatcgtc gaaacagcaa aagaatatgt
     6841 tcctctatta aggaaacaag cagacttggt aatcgttagt taccacggcg gatttgaaaa
     6901 agacttgtta accggtgagg acacagaagc gctaactgga gagaatgaag gctacgagct
     6961 attgcagcag gttgaaggca ttgacgccct tttcaccggt catcagcacc ggaaaattgc
     7021 tgaaacggtt catggagttc ccgttgttca accaggctat cgtggcgagt tcatcggtga
     7081 aattcgtctt accgtcgaga aaaaagaggg gcaagtggtt gttacagatc accaggttgc
     7141 tttacactca gttgaaaaat cagagcctga tccagaaatt cttgcagcga tcgtaccggt
     7201 agaagaaaaa ctagagaaat ggttggatca acccttaggt aaagtgaacg gaaatatgcg
     7261 tattaatgat ccgatgcagg ctcgtcttga ggaacatccc tatgtagagt ttattaatcg
     7321 tgtacaatta tttgccagcg gctcagagat ttctggaact gcgttattca ataatgaagg
     7381 aaaaggcttt ggtgagacga tcacgatgcg ggacgtcatc accaactata tttatccgaa
     7441 tactctggca gtgattcaaa taaatggtgc agatttgaga gcggcgttgg agcagaccgc
     7501 aaattattta gctgtagaaa ataaccaaat cgtattcaat ccagccttta ttcagcctaa
     7561 gccccagtac tacaattatg acatgtatga agggattcag tacactattg atatgaaaca
     7621 gcctgttgga caacggatta ccgaacttcg ttttaaaggt caccccgttc ttccgcaaca
     7681 aatgctggaa gtggtgacaa atcaatatcg ggcaatcggc ggaggaaatt atgcgatgtt
     7741 tggtgcggag aaaattgtcc gagaaatcca aatcgacatg accgaattaa ttgccgacta
     7801 tttgaatatt cacccacgca tcgaagcaaa agtgaatcat aattttcaag tgattttgta
     7861 gtatataaat aaaaaattca aaaaggtgtg agcagttttt tgcccacacc tttgttatgc
     7921 caaattgtta ggctgtctga tttccatcca gccattcttt agccagcgat ctacctcatc
     7981 aaaacaaaaa attttcctct gatggttcct taaataagac tattttgatc ctttttccat
     8041 gaataagatt ttgcatcaga caatacttca tttctgaaaa agttgcggca ccaatatagc
     8101 ctaatacccc tttccgatct tgattaaata caaaaaataa atccgctgct gcgatattta
     8161 aaaaaaagtc gtcaagattt ctggatactc tttgtctagg atcggacatg agcgaggata
     8221 atcaattaat tcaatgcctt ttttactgaa atctttttcc atatctcgat attcttcttg
     8281 gaaaacaata cttccagaaa taacagcttt cacaaaattg gcctccattt ttttattttt
     8341 cagacactcc accataccgg atacctctca aagaaaagac atagcaaaaa tcaatagcag
     8401 ctatctctcc acggtttttt actggtaatt ttatttttct tttggaaatt gtatcaggaa
     8461 ggtcgttcct tcattttttc ggctggtcac ttcaattgaa ccgtggtgca ggcgcactag
     8521 ttccttcacg attgataatc caagacctga ctcgcctatc tttgtatttt tacttgacgg
     8581 atcggcttta taatagcgat cccaaatgtt tttttgttcc tcttcaatca tgccgatgcc
     8641 attgtcctcg acagtaataa atacaggctc tttgtcaaat gatgttggta aagagaaatc
     8701 ttagaataat ttggacagaa cagaaagaag aaactctctt tctgttctgt ttatttggtt
     8761 aaaaatgttt tgccatgacg aatcatgatc cgtagtttga aattgtaaaa gctgcgaaag
     8821 ccgtacgcat tgcgttttaa tactttgatg tggttgttta aacattccaa tggtccattt
     8881 gaatagctgt agtgaagcgc attttggatc tgtggcagaa actttttaaa cgttcgtaaa
     8941 gtggttgtat aggtttcagg aagaccgctg acttctctgt ttaaacagtc ctcaaataat
     9001 gactgatctc ttttctttac cgcataaaga atgtcttggt ataactgata gccttggcgc
     9061 aggttgggac aagcagataa cagtcgatcg acaatctcac tttcagtcaa ataatcttta
     9121 aaactggcac gccaaaccct tttttcgtag tttaactttg cttcttcttt ttgtaggagt
     9181 ttccaatagc gtttcaattt tttccctaac cctcgttgag agtgggaagt tcctttaagc
     9241 cgtgccgtgg tttctttcac tcgatgattc aagaaggttc tgccaatatg ttggacgatg
     9301 tggaaacggt cgatgatcaa ctgagcattc gggaaaagct ttttcaccaa tgaaacataa
     9361 ggcgcataca tatcaataac aatccattga acggcttcac gagtcgctaa tgaaaaccga
     9421 gaaaaatagc attctaaata aggcaagcgt cgattttcga caatatccaa taatgcatgg
     9481 ctttgtccgt ccatcatgat aaaactcatt gcactggcaa cacttcgaac cgatttgaat
     9541 tcatcaaagc ataagacctt tggcaaagcc tgtttgaaag gattcaatgg gcggtagaat
     9601 cgcttcatga cacgatagac cgaagaagtg gaaatatttt tctgacgagc gatttccgtc
     9661 atggaagtat tctcttttaa gcgagcaatg acagaccaac gaactttttg actaatgaaa
     9721 cagtgttttt cagcaacaga ggtatccgct acgaatgttc tttggcaaga cttacattta
     9781 aatcgttgtt tcttcaactc cagataagtg cgatactcag acgcatcatt cagtaacaac
     9841 cgaacggttg tcgtccccca acgaatgatc ctagagggga cgacacaaag acaatgggaa
     9901 cactcttctg gcttgtacag aagagttcct ttgtaaatca tggatttgag tcaatatctt
     9961 ggacacattt tagtagagac ggttaagcca ttttttcttt tgctaattct tcaaattcct
    10021 gtggggttag atagtgaatc gagctatgga ttcgatttcg gttataaaat ccttcaatgt
    10081 agctaaatag cgctcgattt gcttcttcaa aatctgagta gctagtcgtg tagacttctt
    10141 cttttttcaa tgaggcatgg aaagattcga ttccagcatt atcataaggt gttccttttc
    10201 tactataaga atgccttatt ttgttggttt taagccattg ttccacttct cttgctgtgt
    10261 attggctgcc caagtcagtg tgtagaatca ttccttctgg aatgtgataa tgtatttttg
    10321 ctttattcaa tgtttgaatg acacaatcca cagtcattcg ttttgaaaat gtataactaa
    10381 taattttttt cgtgtgtaaa tccatgattg aagataagta acaccaacca tttttcttag
    10441 taggaatgta cgtaatgtca gctgcccatt tctcacagat cgtttcagta gaaaagtcct
    10501 gatttaagag attctctttt gaaatgatcg gtttattaga cctttgaggt ctatattttt
    10561 taactacaat cgaccttaaa tttaattgtt tcattagctt ttggacatgt ttaagagata
    10621 ctgaaatccc ttcttttaat aaaacttgat ggatctttgt ggcaccatag cgttgtttat
    10681 tcgtaaaaaa aattgttgaa atcttctttt tcaacttatt atttctgatt tctgtagctg
    10741 tcaacggttt attcttataa aaataataga cacttctagg gatttctaat aaccgacata
    10801 acaaagaaac agtgtaatcc ttacaccatt tttcaataaa ttgaacaagc gaatttaggt
    10861 tttccgagag aatatggctg cggctttttt taagatttca agttcctcat ttaaacgagc
    10921 attttctttt ctcagttcag ctacttcttt tccagtcagt cctgtggact gatcaggtaa
    10981 atataaattc ttccatttgt aaatcgttgc ttcagataag ccatattctt tcgctaaccc
    11041 tcgaacagaa cgtccagttt gattcagttc aacaatcatt tgtttaaaat tttcttcata
    11101 tcgggtcata aaaattcatc ctctcgtaga taagtttaat ttattcttgt ctctacgaaa
    11161 agctgtctaa gatttgatac taacaccaag ttatctttag aaccatttca gatacatatg
    11221 aaacaattac atatgatttt ttcataaaaa aacagtactg tctagtagta taaaatatac
    11281 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
    11341 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
    11401 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
    11461 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
    11521 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
    11581 aatctttttt cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
    11641 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
    11701 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
    11761 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
    11821 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
    11881 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
    11941 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
    12001 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
    12061 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
    12121 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
    12181 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
    12241 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
    12301 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
    12361 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
    12421 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
    12481 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
    12541 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
    12601 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
    12661 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
    12721 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
    12781 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
    12841 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
    12901 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
    12961 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
    13021 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
    13081 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
    13141 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
    13201 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
    13261 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
    13321 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
    13381 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
    13441 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
    13501 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
    13561 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
    13621 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
    13681 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
    13741 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
    13801 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
    13861 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
    13921 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
    13981 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
    14041 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
    14101 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
    14161 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
    14221 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
    14281 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
    14341 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
    14401 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
    14461 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
    14521 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
    14581 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
    14641 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
    14701 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
    14761 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
    14821 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
    14881 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
    14941 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
    15001 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
    15061 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
    15121 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
    15181 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
    15241 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
    15301 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
    15361 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
    15421 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
    15481 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
    15541 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
    15601 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
    15661 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
    15721 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
    15781 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
    15841 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
    15901 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
    15961 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
    16021 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
    16081 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
    16141 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
    16201 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
    16261 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
    16321 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
    16381 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
    16441 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
    16501 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
    16561 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
    16621 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
    16681 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
    16741 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
    16801 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
    16861 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
    16921 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
    16981 ggtatcgata gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact
    17041 gatttatttc taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata
    17101 ccaaacatat ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa
    17161 taacataaaa attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa
    17221 tttataggaa actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga
    17281 cggtatgcat aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca
    17341 catatatacg gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt
    17401 ctaatatagc tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat
    17461 cacatgatcg atttaagttg tactgttctt gtaaaatctg tgttaccaat ggtttcgatg
    17521 acttctctaa tccagatgta cctgtcattt ttgaaaaaat gatacctata atagccaaga
    17581 caacgataac tataatgata aacttcttgt tcatatttac tttattcaac tttttatctc
    17641 ctatctccaa aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta
    17701 caccaaagat tgctccagct acccctgcta tcatcaaaat cgtacccatt tgttcatctt
    17761 tatctcgata taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg
    17821 ctaacgctac taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc
    17881 catgtacgat ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgcgt
    17941 ctgctccagt tgtctcatct gtttcttgta gtgctccgca aaactggcaa tacttacttt
    18001 catctgttat ttctttgcca cattttctac aatacatgtc ttctcctcct aaaaacattc
    18061 aattgtttat tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg
    18121 gtacacttaa tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg
    18181 tttgccacga acttacacat tgctgtgtaa cattcatttt tttgctaact ccgtttgaga
    18241 aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga ttctcccctc
    18301 ctgttttatt atatacaaca atatgatgta tttcaactca taaaatataa aaaaacttca
    18361 aataccatta caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa
    18421 acagaaacaa tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa
    18481 agaattagct gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt
    18541 aacagagcct aacctcgata gcttaaccgc tctttctaga gccctaggaa cccctgtaag
    18601 ccattttcta gacaatgttg tcgtagatta tgaggaagag tttttggctc tctatcgttc
    18661 tcttagcact gaagatgcag ttcgaacaat tgactatatg aagctattaa aaaggcaaga
    18721 aaatgaacgc aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg
    18781 tgggtgtatt gagatgaaat aagattaaga gctatggata acctgtctag tttaatacaa
    18841 ttgggtcttt tctcttcctt actctccgtc catcatctct tgttacactt gtaaattgat
    18901 aaagaggaac taattcaact cctgataata tcaattcttt tattacttca tctatccctg
    18961 ctccttttgc taggtagact aaaaaactac tacttgcttt cacattgtat agtctcttaa
    19021 tatccttaga agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgttttctt
    19081 ttaaagcatt ttccacgctg ttattcccaa cattacgtag acgttcacaa acagtgttag
    19141 taagttcttt ctctgtcatt agatatgttc cttgccacga taagccctgt aaaacttcat
    19201 gtgcttcatc taaaatcaat tgtttttcct tgctaagttc tgatcgggaa ttttgaggaa
    19261 atactctttt agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt
    19321 gatatgtaaa atgaaatttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca
    19381 cagaagttgc attaaaaacg tctaatcgat agacattgct tctcttatga ttaaagtttc
    19441 ctaacgcttt ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt
    19501 catcatctaa tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt
    19561 tatctatgtc ataagtttta ccatagtaaa aactcatttt tttagctaat tgttcccccg
    19621 acaaaataca agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga
    19681 catcatagac acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt
    19741 tcattgattc aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg
    19801 taggatacaa gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct
    19861 ttaaaattag catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac
    19921 aataaaaaat ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt
    19981 tgtgaaacag acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta
    20041 acaatctttc ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa
    20101 accactgttt catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat
    20161 tgactagttc atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa
    20221 aatcaatcgg aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat
    20281 ctgacttata tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg
    20341 ggatgttctt tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt
    20401 cttttaatag aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt
    20461 gtatcgcaaa cgcagaatta tgaactaaat tgatagataa aaccttttct atgcttaagt
    20521 atctactatc atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat
    20581 cgacatccag aataattact ctggtttcca caaaactctc cttgtcattg cctttattct
    20641 ttgtcttgct tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg
    20701 taacttctct atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa
    20761 ctggcttctc taataatttt tctttatcta cataaattgg aatattaatc attgatgttt
    20821 ccatttgtgt ttcctcctta taaaattctg ttaaagacta aaacatacag ataataaaaa
    20881 caaactcttt attactatat gttttagtct tatttgttta ctcaaccttt ataccgttcc
    20941 atgaaactat caatatcaga ttttttaaat ctaatgaccg tatcaatttt tataacaggt
    21001 aacccgtatt gagaaacata ttttctaatc gttcctactg atacacctag atattcactt
    21061 gcctgctgat agtccatata ctctagagtg actccagaaa taatgtcttc tagcataggc
    21121 tttagcactg taattatctc tttcacttgt tgttcaactt gtatagaaac aatgctatca
    21181 atgatttgta gagacatatt tccccctcct ttcgtgattg cttgaacaaa ttttctaaga
    21241 attatcttcg tattttttgc tcaaaataaa agtattttgt tattaaccga ttaatcaaac
    21301 aaaaaatgat taccagttca tcgacataca cataatacct cgataaaaaa gaggtgtcaa
    21361 tatctttgta aaatttattt acaacacccc tttttcgagg tatactaatt ttaggaggtg
    21421 tggtcaatat gataaaacgt ccaccaataa attatttaga acgaaaaaag atactcggaa
    21481 caaaaataaa agctataaga aagagtaaaa aattaacaca accagcattt ggtcttatga
    21541 ttaacaacgg tcaattaatt gacaaaaaaa ctatctacga atgggagaag ggaacctatt
    21601 taccaatacc agagcgatta tcccgaattg ctgatttggg aaatatgagt atagaggagt
    21661 tagtttgtgg caatgtagaa gaatatattt tgggaattat tctttataga gatagcattg
    21721 tactagatgg tataacattt ccagataaaa acctgttcca gcatcttcgt caacagttcc
    21781 caccagtaca ctctaattta gatacatggc tggatagata ttccaaatta gaaccagaga
    21841 tgcaagaatt tattgctaac aagacttgta ataaagttaa aaatgaaaaa attagtcttt
    21901 tcaatatctt aaaaatagaa gaattattca tcaatgctat tgtagaagaa tttgacaata
    21961 acattctttt cctcactagc agtatagaag aactactaga aagaatggta gatgaatggc
    22021 tacctattca attgaaagac atgtcttatc ccgaagaagc tgtgagagaa attactgata
    22081 atataaacaa gctagaacaa acgatttcat caattgggaa aaaatacact aagaaaaaaa
    22141 tgaagggagg tgataccatc tagtggtaag tactccttgt aaactaagag gagatgattt
    22201 catggcaaaa atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg
    22261 acacattgcc accgatccac gaactggtga aaaaataaat actacgagaa gaggttttag
    22321 tagcaaacaa gaagctcaaa aagattatga cgagtacaga catcaaatat tatacggtgt
    22381 taaaaagaaa gcacctgata tgacatttga agacctgtat aatgaatgga taactcatca
    22441 gcgtaccagt gttaaagctt caacaatcgc aatctcagta agatacgcaa ataatcaaat
    22501 acttccagca tttggtaagc ttaaactatc gaatatctct gtcccatatt gtcaaaaagt
    22561 ggtagatgaa tggcacagta aatatgaaag ttacgattat atgagaaaac agactgcaca
    22621 aattttacgc tacggggttg caatgcaata tattgacaat aatcctatgg aaaaaacact
    22681 tcttccacga aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa
    22741 taatttactg gatgctttta aagattttgg taacatgaaa caatacgcct tcttcagatt
    22801 attggcatac acaggtatga gaaagagtga agtactcgct ttacagtgga aagacataga
    22861 cacgtttaat aaagaactac atgtcaataa aactcttgct gtagatgaat ttggtaaagt
    22921 tataattcaa tctccgaaaa caagagcttc tagacgagtt atctctttag atactgagac
    22981 tctttctatc ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa aacttggata
    23041 caatactagc tctaaagagc aacatgtttt tacaacagtt aaaaacactc tatacattcc
    23101 aaacactgtt aatgattggt taagatatat tttgaaaaaa tataacttgc ctcgtattac
    23161 acctcatggg tttagacata cacatgctag tttattatta gaagcaggag aatctgtcaa
    23221 ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg acattagaca tctatgcaca
    23281 tattacaaat aatgcaccta agaaaacagg tcaagacttt gctgatatga tggctcatca
    23341 gtaaaagagc tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa aaaagctaga
    23401 acccttatat at
//
LOCUS       DAZKMK010000040        22711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000040 DAZKMK010000000
VERSION     DAZKMK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.040"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG8V1_001480"
     CDS             complement(11..1942)
                     /locus_tag="ACG8V1_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0329576.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8V1_001481"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8V1_001481"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0329577.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG8V1_001482"
     CDS             complement(2871..3623)
                     /locus_tag="ACG8V1_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0329578.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG8V1_001483"
     CDS             complement(3782..3991)
                     /locus_tag="ACG8V1_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329579.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG8V1_001484"
     CDS             complement(4002..5330)
                     /locus_tag="ACG8V1_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329580.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG8V1_001485"
     CDS             5466..5621
                     /locus_tag="ACG8V1_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0329581.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8V1_001486"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8V1_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0329582.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8V1_001487"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8V1_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0329583.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8V1_001488"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8V1_001488"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0329584.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG8V1_001489"
     CDS             complement(9305..10237)
                     /locus_tag="ACG8V1_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329585.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG8V1_001490"
     CDS             complement(10394..11053)
                     /locus_tag="ACG8V1_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0329586.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8V1_001491"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8V1_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0329587.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG8V1_001492"
     CDS             complement(12094..12384)
                     /locus_tag="ACG8V1_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329588.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8V1_001493"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8V1_001493"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0329589.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG8V1_001494"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG8V1_001494"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0329590.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACG8V1_001495"
     CDS             complement(16049..17218)
                     /locus_tag="ACG8V1_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0329591.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG8V1_001496"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG8V1_001496"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0329592.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG8V1_001497"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG8V1_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0329593.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACG8V1_001498"
     CDS             22182..22703
                     /locus_tag="ACG8V1_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0329594.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6325 a         4931 c         4036 g         7419 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata a
//
LOCUS       DAZKMK010000041        22677 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000041 DAZKMK010000000
VERSION     DAZKMK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.041"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..878
                     /locus_tag="ACG8V1_001499"
     CDS             177..878
                     /locus_tag="ACG8V1_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0329595.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1487..2503)
                     /gene="tsaD"
                     /locus_tag="ACG8V1_001500"
     CDS             complement(1487..2503)
                     /gene="tsaD"
                     /locus_tag="ACG8V1_001500"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0329596.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2500..2964)
                     /gene="rimI"
                     /locus_tag="ACG8V1_001501"
     CDS             complement(2500..2964)
                     /gene="rimI"
                     /locus_tag="ACG8V1_001501"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0329597.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2971..3513)
                     /gene="rimI"
                     /locus_tag="ACG8V1_001502"
     CDS             complement(2971..3513)
                     /gene="rimI"
                     /locus_tag="ACG8V1_001502"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0329598.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3497..4219)
                     /gene="tsaB"
                     /locus_tag="ACG8V1_001503"
     CDS             complement(3497..4219)
                     /gene="tsaB"
                     /locus_tag="ACG8V1_001503"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0329599.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4410..5390)
                     /locus_tag="ACG8V1_001504"
     CDS             complement(4410..5390)
                     /locus_tag="ACG8V1_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0329600.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5414..6898)
                     /gene="galT"
                     /locus_tag="ACG8V1_001505"
     CDS             complement(5414..6898)
                     /gene="galT"
                     /locus_tag="ACG8V1_001505"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0329601.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6910..7899)
                     /gene="galE"
                     /locus_tag="ACG8V1_001506"
     CDS             complement(6910..7899)
                     /gene="galE"
                     /locus_tag="ACG8V1_001506"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0329602.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8147..8314
                     /locus_tag="ACG8V1_001507"
     CDS             8147..8314
                     /locus_tag="ACG8V1_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0329603.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8376..10187)
                     /locus_tag="ACG8V1_001508"
     CDS             complement(8376..10187)
                     /locus_tag="ACG8V1_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0329604.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10184..10549)
                     /locus_tag="ACG8V1_001509"
     CDS             complement(10184..10549)
                     /locus_tag="ACG8V1_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0329605.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10712..11107)
                     /locus_tag="ACG8V1_001510"
     CDS             complement(10712..11107)
                     /locus_tag="ACG8V1_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0329606.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11125..12087)
                     /locus_tag="ACG8V1_001511"
     CDS             complement(11125..12087)
                     /locus_tag="ACG8V1_001511"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0329607.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12087..12299)
                     /locus_tag="ACG8V1_001512"
     CDS             complement(12087..12299)
                     /locus_tag="ACG8V1_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0329608.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12320..13018)
                     /locus_tag="ACG8V1_001513"
     CDS             complement(12320..13018)
                     /locus_tag="ACG8V1_001513"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0329609.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13038..13580)
                     /locus_tag="ACG8V1_001514"
     CDS             complement(13038..13580)
                     /locus_tag="ACG8V1_001514"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0329610.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13712..14716)
                     /locus_tag="ACG8V1_001515"
     CDS             complement(13712..14716)
                     /locus_tag="ACG8V1_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0329611.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14713..15702)
                     /locus_tag="ACG8V1_001516"
     CDS             complement(14713..15702)
                     /locus_tag="ACG8V1_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0329612.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15699..16505)
                     /locus_tag="ACG8V1_001517"
     CDS             complement(15699..16505)
                     /locus_tag="ACG8V1_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329613.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16671..17627
                     /locus_tag="ACG8V1_001518"
     CDS             16671..17627
                     /locus_tag="ACG8V1_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0329614.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17704..18222)
                     /locus_tag="ACG8V1_001519"
     CDS             complement(17704..18222)
                     /locus_tag="ACG8V1_001519"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0329615.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18310..18459)
                     /gene="rpmG"
                     /locus_tag="ACG8V1_001520"
     CDS             complement(18310..18459)
                     /gene="rpmG"
                     /locus_tag="ACG8V1_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0329616.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18687..19133
                     /locus_tag="ACG8V1_001521"
     CDS             18687..19133
                     /locus_tag="ACG8V1_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0329617.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19328..21223)
                     /gene="asnB"
                     /locus_tag="ACG8V1_001522"
     CDS             complement(19328..21223)
                     /gene="asnB"
                     /locus_tag="ACG8V1_001522"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0329618.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(21548..22522)
                     /gene="bsh"
                     /locus_tag="ACG8V1_001523"
     CDS             complement(21548..22522)
                     /gene="bsh"
                     /locus_tag="ACG8V1_001523"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0329619.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         6856 a         4780 c         3871 g         7170 t
ORIGIN      
        1 cacaaatcat tcgcttcaaa acaaattatt caataccatt gtatattttt tacataataa
       61 caaattatat ttgtaaagta ttaaaacttg tttctaatgt tggtatgtta aaactttaca
      121 aattctttaa aaaaagtgac aataaatata gtgaacaata aggagaggac tttttaatgc
      181 gtgttaataa aatcgatttt cacttttcaa aactcagata tcaacctgat tttgaacatt
      241 tctcggatga agagttccaa cagatcaagg aacatactgt tcttcgttct tataaaaaag
      301 gacagatcct tttcgacgat ggtgatccca gacaacggtt gtatatcttg accaaaggac
      361 tcgttcgttt agaacgttat gatgaaagtg caacttatta ctactatgac tatgtgaata
      421 caaataacct ttttcctatg tcaggaatgt ttggagaaga gacctacact tatacagctc
      481 aagcgatgac tgatattgaa acattctacc ttcctgtttc ctactatgaa aaattagcac
      541 gaaaaaatcc cgaacagttg atctatatga tacaaaaaat ttctaaagta gttgaaatac
      601 atgaattgcg tatccaaatc ggattgacct ctagcgcctt tgaccgggta aaacaaaatt
      661 tattcatttt gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc accattcctt
      721 atccgatcac cttaaaagaa ttagcagtga atagcggcac gacacgtgaa actgctggac
      781 aagtaatcaa acaactacgt gattccgggc tgttagatta tgacaaaaag cgttttactt
      841 tctgtaagac agctactact ccagctatga gagaataacg cgaacaaaaa taagcagtga
      901 ccctgataaa tatcagggtc actgcttatt ttcctcccta aaacttctga aagatccggc
      961 agttttagat cactggacaa gactcaaaga ttttctatgt gtgtttcagt tatgtaatat
     1021 tcactgtttg caaaaaatac tgttagatta actagatgaa gcgtcttgtc ctcatccgat
     1081 ctagattatt cgatattcct cgacaataaa cgattactca gtgagggcaa tcagaggaac
     1141 ggataatgat aaataagctg taaaaaatag caaaataaca ataatgatta aaagtatgta
     1201 aaaaataggg ctttgacaaa aacgtgtcac agccctattt cgattaaacg gtgtgtacct
     1261 gtcaactctt cggtcacaag tcataatgtt cataaacatg aggagctgtt ttttgaaatt
     1321 gttccttgtg aatcagagtt aaacgacagg cttacgaact cttgattaaa cggcgttcaa
     1381 aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa catgagaagc cgtttttcct
     1441 gggctgttct tattacttga tgcagggcag ctttttcaca acctctttat tcagataaaa
     1501 ccaagcttgg atcagcattc aactgatagc tagcaaaatt tcttttttgc atttccacat
     1561 gtgctgctgc tccaatcatt gcagcattat ctccacaaag tcgaagaggg ggaatcacta
     1621 gttctacctc agggagttta gttgccaaag catcctgcaa tccttcacgc aacccttgat
     1681 tggccgctac acctccagct ataatcagtt gcttgactgg ataattttga caagctcgaa
     1741 gtgttttagt aatcaataca tcgatcacac ttgcttgaaa gctagctgct aaatcatttt
     1801 tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa attgataaat gcacttttta
     1861 atccgctaaa actgaaatca taattatctt catggatcat cgctcgcgga aaatggtaat
     1921 tatcttttcc ctgatgagct agctgatcga tttcttttcc gcttgggtaa gaaagaccta
     1981 atactcttcc aactttgtca taagcttctc ctgctgcgtc atctcttgtc tccccaatga
     2041 tttcatatga tccatcttct tgcatataga ctaattctgt atgtcctccg ctaacaagaa
     2101 gtgccatcaa tggaaactgg aatggtttaa ccagacgggc tgcatagata tgccctgcca
     2161 tatggtttac aggaataagc ggcaattggt gtgcccaagc aaaagcctta gctgcagaaa
     2221 taccaatcag caacgagccg acaagtcctg gtccataagt gacagcgact gccgataaat
     2281 cttctgccga cacaccagct tctaccagtg catcttccaa gcataaggtg atttgttcta
     2341 cgtgatggcg gctagctact tcaggtacta cgccaccaaa acgtttatga ctgtttattt
     2401 gagaagcaac gatgttagat aagatctctg ttccatttct aacgaccgct acactcgttt
     2461 catcacaact tgattcaatc gccagaatca attcttcatt catttcatcg tctccgtttt
     2521 taactttctg ctcataatga tcgcatcttc cgtcggattg cgataatatg atttccgttt
     2581 acctaatata cgaaactttt ctgattcata aagttttctt gctgcttcat tagatagacg
     2641 tacttctaaa aaaacatgca gcgaatcttt tatttgttcc gtaatcagta attgtaacaa
     2701 atgccgtgca tgcccttttc cttgttctga aactgcaaca gcaatatttg tgatctcgac
     2761 ttcatctatt actttcgagt atccaataaa gccacagatc tggttatctt cagctaaaag
     2821 cagataatca gagtgaggct gttggatgtc tgccaaaaac tgcattttcg tccaaggtga
     2881 accatgtgga taagcatttt cgctaatcat ccatacgcat tctgccaagt acgcttcatc
     2941 aaaatctttt tttctgcaaa tcaactaatc tcactcattt tctaaaagtt ttatcatatc
     3001 caacgcatct tcttttgttt cagtgtaata tctttttttg atcgttgttg cttgaaaccc
     3061 caatttccga taaagtcttt gtgcatcttg attactcata cgcacttcta atgacatcgt
     3121 ctcacagcga ttcattatag caaagttctc gatttcgtca ataagcagcg aaccgattcg
     3181 tttacgctga tatgccggtc gaacggcaat attcgtgata tggcaatcta aaccgaacgc
     3241 tcttgaaccg ataaaaccaa tcattcttcc attatcaaag atacctagat atagatgagg
     3301 aagcggtgac ttcaattctg atataaaggc actttttgtc catggcaatt cgccattata
     3361 gacttctcgt tcgacatcca gcagctcttt gatatcttct gtagtcaatt ctcttagtac
     3421 gtaacgacga tcgatttgtt cgactgtttt ttctgtataa atcattttat cttgaaaaaa
     3481 gcttctggca tacgctctaa attttttcaa cataactttc gtctccaggt gtgtgcgtct
     3541 ccaaccattt ttcttctgct tctactcgtt ttaaatagcg aggaagaaaa gcttgtttct
     3601 ctgcaactgg ttcaactaaa gcacctaatt gagccacagt tacaccactt ggaatatccc
     3661 atgctggaac tgtattaatc atcgcactag gcaatatttc ctttatttca gagaagaatt
     3721 tgtgacaatc actcccgacg aaataaatag cttcattcag cttttgtagt tgtgtgaaca
     3781 aatcagaagc agaaatatgt acatcttcaa gtacagtttc taatctattt tctttccagc
     3841 ggtatgcccc agcgtataca ttttttcttc gagcatcaaa catgggcaca ataatattat
     3901 ccacaccgat gcaattagcc gcgatggttt tcagactaga tactcctact agctctttat
     3961 ctaatgtata agcaatcgtt tttgctgtcg ttacacctat acgaagccct gtataagaac
     4021 cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg acttatttga agatctgcaa
     4081 acaattggtc gattgcaggc atcagtgtta cactgtggtt gcgtttgata tttgtttgta
     4141 tttgtccaag aatttgttcg tcttctacca caccgatcgc caaagtttgg tttgcagtat
     4201 ctattcctaa tgtaatcata tatttgatcc tttctgctat gacattctcg ccttccattg
     4261 tagcatactt ttttattctg aggatgaaaa taccaaaggc aaaactagta caataataca
     4321 tgattatctg ctggcgggca aattttgccc cttcctgaaa cacaaaaaag ctgcggtatc
     4381 actccatttt cccacagctt ttccatcttt tatttcgttg attctcgaat aatcaactct
     4441 gtttccacaa taatctttct tgaaaccggt gctttggaat tacttaattg aagaatcgtc
     4501 tccactgcta gctctcccat ccattcggta taaactttta cagtagaaag cgagggcgtt
     4561 acatatttcg ctacactgac atcattgaag cccatcacag aaatatcttc tggtaccact
     4621 aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta atgcatcatt agctgcaaaa
     4681 agcgcatcag gaacatgcgg attcgttttt aaatactgct tcactgcttg atatcctgac
     4741 tcgacagaaa aatctgcttc gataattgga ccttcgtcgt agcctttttc tgccaaaact
     4801 tgttgaaatg ccataattct gggatctttt agccgctgat ggtttttctt cgtatattct
     4861 gttccagcaa gcatagcgat atgttgcgct ttatgggaga caaagtgttc aatgactttt
     4921 tgaacacttg acttaaagtc gacaactaga gagtcgattc cttgagaagt gccgtcagaa
     4981 tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta ataactgagc ttcgtcgaat
     5041 tttcctagtg cgatcgttcc atctgccgga agatcactaa tggaatccca cgtttctttc
     5101 aataaagtaa cgtttgcttc ttctgccttt ttttcgattc ctaggcgaat agataaataa
     5161 taaagatctt caagctcttc cgtatcgtcg taccactgga tcaggcgaag cgtttgctta
     5221 gtatttgctg catttttacg atgtttcgta taatttaagc gttctgctgc ttcaaaaatt
     5281 ttttgctttg tggctaagcc aacggaaaga tcaggatcat agttgagaac tcttgaaaca
     5341 gtagcaggag aaactcctgc caactgcgca atgtctttaa ttgttgccat ttgtctctcc
     5401 tcctttttta cgtttataaa atcgcagtaa atcgttcaaa cgctgcctgt cctttttcat
     5461 ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa aacctgacct acagattttt
     5521 gtacgacatc ggtgaccgta tctttcgtca aatcaggatg ttcttttttt aattgttctg
     5581 cccacggacg atgatattca gccatctggt tttcttctcc taataagaaa tgctctactt
     5641 ctactagttc tggtttcaaa cgaggtggta aaacagcaag ccccattact tcgatcaacc
     5701 caatgttctc ctttttgata tgttggacat ctttatgtgg atgaaaaatc ccatcaggat
     5761 gttcttctga aacattgttg tctcgaagga caaggtccaa ctcaaagtaa ttgtcccttc
     5821 ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc tgctgaaaaa gctctgatag
     5881 atactgtttc atccgaatat gctttccatt tttccaatac tgagttagct gcttcaataa
     5941 gcaagttttt ctctgttgat tgcaaacgga tcacagacat cggccatttc acgactcctg
     6001 cgatgacttg cggataatct tctagtttga taagtttttc tatcggtgct tttgccatag
     6061 gaaattcatg acgtcctgcc tgataatgat catgagaaag aatcgatcct ccaacaattg
     6121 gtaagtcagc attcgaacca acaaagtaat gaggcaagac ttcagtaatc cgaagcagtc
     6181 tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg ttctgataaa atgatcgaat
     6241 gttcattgta ataagcataa ggggaatatt ggaatcccca gctttcaccg tctagattca
     6301 ttcgaataat ccggtgattg gtgcgggctg gatagttcaa gcgacctcta tatccttcgt
     6361 tttccatgca aagcatgcag gtaggatagt ctacttttac cgcctcgcgt tctgctgcga
     6421 tttgttttgg atctttttct ggcttagata aattgattgt gatttccagt tcaccatact
     6481 ccgactctgc cggaaatttg atgttttttg cgatggctct tgttttgatg tagtcatttt
     6541 tcttacttaa attaaagaaa tactcagttg cttcctctgg gcttttcgaa tagtgctgtg
     6601 caaagaatgc attgacgact gaaggtggcg gtgtgagaaa atccattaat tgcgcttcaa
     6661 aaatctcttg ttctgctggc aattctgaaa gaacaccatt ttctcttgcc ttagcgacaa
     6721 gctgatccaa taagtctaca gattcttcct taactggtct tacttcaatg ctttctagag
     6781 catcttctcc taccatccct aaaagacgat tctgtaaata taagcggtcc atttccatcc
     6841 atccgccagc ttcgatcgca agtgtggcaa aatcacaaat cgtttgactg attgacatat
     6901 ctcattcccc tattcttcat aaccatttgg atggctgaca tgccaatccc atgctgtttt
     6961 gataatcgct ttgatatctg tgtattcagg tttccagccc agaatctctc tcgctttttc
     7021 acttgaagcg actaatcggc taggatctcc agctcttctt ggtgctattt ctgctggaat
     7081 ttcttttcct gtcacttctc tagctgcttc tagcatttcc ttgactgagt atcctttgct
     7141 gctgcctaaa ttgaagaagt tgctttcatt tccttctttc aagtattcca aagctaaaat
     7201 atgtgcagca atcaaatctt ctacttgaac ataatctctg atacatgttc catctggtgt
     7261 atcataatcg tctccataaa cagccaaggc ttttctttga ccaagagcta cttgtaaaat
     7321 gattgggaca agatgcgttt ctggtgtatg atcttctccg atagatgcat ctgcttttgc
     7381 tccggctaca ttgaagtagc gcaaagcgac atagcgcatg ccatatgctt ggtcacacca
     7441 cttcatcatt ttttccatca tcaatttact ttcaccatac ggatttttag gattagctgg
     7501 tgtatcttct gtgattggag actctttcgg ctctccataa gttgctgcag tagaagagaa
     7561 tacgatttta ttcacgttga actcgtgcat gacttcaagt aagatttgca taccgtatac
     7621 gttgttgttg aaatacatca atggtttttc gacagattct cctactaaag agcttgcagc
     7681 aaaatggatt actccttcaa tcggttcctt ttcaaatacg gaacgtagaa attctttatc
     7741 ccggatgtct ccttcataaa aatgagcatc tggatgtact gcttgtttgt gacctgtgag
     7801 caaattatct accacagcta cttgataccc cttttgaact agctggtcta cagcgtgcga
     7861 accgatataa ccggctccgc ctagtactaa aattgtcata aaaaacactc ctttgattag
     7921 taaaattgtg tttgttgatt ctatataatt taaaaaacga agtttacttt tatggtgaaa
     7981 acaaacaaag taaaatttcg taaattcata tggtaaattt ataataaaac atttagtaaa
     8041 cattttcaag gattttttgc aaaggtttac aaacaaacgt aaaactagcc tttttttctc
     8101 ttttgttcca taatagatat aacaaataat aaatgaggtg gattcaatga aaaaatttgt
     8161 atctggaata ttagtaggaa gtctggcaac agccgctgcc gttgctggtt tggttgcttc
     8221 tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa gcaatgatcg aagaaaaccg
     8281 taaaaaagca atgcgtaaac gcgtttctcg ttaataatta tttaaaaata cgcatgaaga
     8341 ctttctaaaa aacagataag tcatcatgcg tatttttatt tgtcagtcaa tataagttgt
     8401 gctattttat ctgcctgtct cagattccat ggatctgtat aaggtctagc tactgctgca
     8461 gttagccaat ccacaccttc aacaggtatt ccatagcaat aaaagtcaga ctggatttcc
     8521 cctgaagcat caatcacgcg attcgtacct cgctcaacat ctatagcttc tgaatgataa
     8581 cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt caaatttttt
     8641 atgacaggat tacttgaacg agaaaagctt gtttgtggca atcgagcttc aatcaaatag
     8701 cgactgctga tttctttatc ttgtagagaa ataacatagc ggttgtgctt ttgctgaata
     8761 tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc taattctttc
     8821 gtccgaatga ttggcggacc gatcgttaaa aagctattta gatgagtaaa ccatccccat
     8881 agtatttctt tgtattcctt agcagaaaaa acgtgccatt caatagcctg ccggattgga
     8941 tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca attgcctttt
     9001 tccgcttctt ttatctgccc ttcaataaat attcggctag cttctgaaaa gggctcattc
     9061 tgataggcaa gaggagtttc taaatacgac caatcccatc tataagggat tagtgctgga
     9121 tgctttccga gacactcctc tgaagagagg gagagaaaat catgctcaaa atttttcttt
     9181 ggaatcggca agtgatattc ttcgatgatt ttcttgtaat aaaacaactc aacgtctttt
     9241 ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca ttgctccagg
     9301 ttttcatttg ttagtaaccg tggccatgcc attgctccgc cttgcttttg atttcttcct
     9361 cgagggtaat aagggagccc ccttcttgat ccactgtata caatcggttc cttgcctgat
     9421 ggatgataga ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga tactgtaaac
     9481 aatccgacat aatcaaaaaa acttaagcct agacctctta aaaagacatt ttctttaggc
     9541 ggtatcttcg aaagatctac atctgcagga ttagaaggaa gctgatacag taaaacattt
     9601 ttctttgcgt agtctaccca cactctttct tcttctgtct catcattttc ccagtgaccg
     9661 cttgctaaaa caagctgatc gatagaatag ctttgctgat ctgtctgtaa aagataacca
     9721 tcttttcttt tgcttacttt ttttactaaa gaataaacaa actgaataga agctggaaat
     9781 tcttcttgca actttgagaa aaaccagcgt tgatagagtc cgtataaaca tcgagtagat
     9841 tgctgatctt tttccagacg attgatttct tctataatta gttgcttatt tttggttacc
     9901 gtctgttcga tataatctgt agcaggtcct ttactccatt gaaacaagtt gggtccctgt
     9961 gttaccaatc ctccactgga caatgtttca tctgtaaaaa gcgtcacttg ttgagaaaca
    10021 gaattcatta ataactccgg tggctgattc aaccgccaaa ctcgacctcc aggaccattg
    10081 ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat tattatggag aatcctttct
    10141 gctacactta atcctctagg tccggcacca atgatcccta ttttcatttg catgcctcca
    10201 tttcgataaa ttttttcaat aaaacagcat ggttctgatg ttcatttttt gcaccataaa
    10261 caaaagtaat gactggatgt tctttcataa gttgactgag tttaaaaata gcttgcttct
    10321 tttcttcttc gaaaatcaat tcttgctggt atttctttgc aaatgaagca aatcgttctg
    10381 gtacatggtc aaaagttttg cgtgcttcat tactaggtgc aatttctttc aaccacagat
    10441 ctaactgcgc tttttctttt gaaattcctc gtggccagaa tctatccacg agtattctat
    10501 agccatcctc tttttcagca tcaaggtaaa cacgttttaa tttgatcata gttgtttcct
    10561 tctttctacc ttttcgctta gcttatatat gtagataata gcacaaaata aaaaaaatga
    10621 acggcacaac tatccattgc gccgttcatg tgatcttggt caaaatctaa tgattgattc
    10681 ggtttttaaa gctgtcatac aatcatctgt tttatacttt tttacttttg atttttccag
    10741 accatccgtc atatccaccc ttaagtaaat aaatctcttg ataacctgct ttacgtaatt
    10801 tatttgctgt acggatgctc aaacttttct tttgatcgta aatatagaca ggctggtctt
    10861 ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt gcacctaaaa
    10921 tatgcccagc atcaaacaca tctttttcgc gaacatcgat tacttgtgct tttctcattc
    10981 cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt actcgtagat
    11041 agacttccca aaggatgatt gcaagcacaa tcaatagcaa aatgatatta attacccata
    11101 aaaccatgta atgcctccgt ttttttaagc aaatttcaag gctaatgagg cagcaccaat
    11161 gacaccagct tcattaccaa gctctgctaa tttgattttt gttgattctc ttacttgcgg
    11221 gaaagtaaat tctttaaaat atttctctac acggctacgt aaaaattctc ctgctgccga
    11281 tactcctcca cctaacacga tacttgacgg atttaatgta ttccccaagt ttccgcaagc
    11341 taaacctaag tagaagcata cgcgatcaac aaccatcaat gcaaacggat cgtcttcttg
    11401 agccatttca aagatatctt tacttgttac ttcttctccg ttgtctagca ttgctttcag
    11461 ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct aggcgtacca cacctgtagc
    11521 gctagataca gtttctaaac atccgcgttt accgcaagta cattcgaacc cttcaggatc
    11581 cacagtgata tgtccgattt ctcctccagc tccgctcacg ccgtgaatca aatttccgcc
    11641 agcaacgata cctccgccaa cacctgttcc tagagtaacg aatacaacgt ctggatcgtt
    11701 ttctccagca cctttccatc gttcacctaa agcagcaaca ttcgcgtcat tatcgatagc
    11761 gaatttgatt cctgttcctg cctcgatcag ttttttcaca aattgaactt ctgtccagtt
    11821 taagttatac gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg gggtacccat
    11881 gccgattccg ataaagtctt cggctttcat attatataaa ttcaaacgat gattgattga
    11941 ttcaatgatt tcaggaacga tatgcttacc atcatccaaa atattggtat cgatgctcca
    12001 tttttgctga atctctcctt ctggtgttaa aattgcaaat tttgctgttg ttccacctaa
    12061 atcaatccca ataatcttct tgtccattac aaatttcctt tctttcggct ttcttcaatt
    12121 cggtgctctc tttttaatat gcgccacgct gtcatgtatg tatcatgatc aatcaaacgg
    12181 ctatcataca gattacgcaa ttcgatagtc attaattcaa tgtcatagat gcgtgcaccc
    12241 acatagataa agatgccaaa ttttttgaac agctgctgta catcatataa gctctccatc
    12301 tttaagcact tccttcatat tatacaagta aaccatattt ttttaaaccc agtaaaatac
    12361 atattacaaa aagaaataca aaaactagtg tagaaagaat cctttggtgt atcgaatagc
    12421 ttgtctgttg tttcggtaga cccaaaacat tccctaacac taatccgcca attagcccac
    12481 cgacatgtcc ccaaacatct acagaggtat ccagaagacc aaaaacaaat gtcaacacaa
    12541 taaataaaag aaaatgtctg actagctgct ggatcgctgg gttatgttta aaatgaaacc
    12601 ctaagataaa caacgcaccg aacaaaccga atatcgaagt acttgcacca ccagacagaa
    12661 cattcgcttc gttaaaagca aagcttgctg tatttcccat tatcccgctg aacatataaa
    12721 tcaagaaaaa acgccaatgg ccatagattg cctcgatttg ctgccccata aaatacagta
    12781 tcagtgagtt cattacgaaa tgcatcagtc caaaatggat gaagattggt gtcactaaac
    12841 gccaccattc attgaagtga acaagataag gaccatacat tccggcataa taagggatct
    12901 caagtcgcgg aaataattcc atcatgagga agaccgctgt ctgtatccct aaaaaagcat
    12961 aggtccataa cggtcgtctt aaccatacac gcaattttat ttgctgttga taattcatga
    13021 aaacctccca tttcatttta gtcaataaac aattgttgga tcttctgatc aaagctctcc
    13081 ggttgccatt gttcattcaa ttgttcacga aaaacaaggc tacatgtttt tttatgaaaa
    13141 ttatttaaat accggtcgta ataaccaccg ccaaaaccga ttcgataacc ggctctattg
    13201 tagataatac ctggtacaat gaggagatcg atcatctccg gcaaatagag cttctctgat
    13261 tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg actgttcatc catttcataa
    13321 aacgccatct gacgtttcgg caaagtttta ggtacaacta ttttcttttg ttctttttct
    13381 gctcgttgga taaccggctg tgtatccagt tcaaattcat tcgataaagt cgtccctatc
    13441 acttttgctg acttccagta ctcgctggcg aatagctgtt tatagattcg ctgttctttt
    13501 tcctttttct tgccactttt ggccagattt ttcagctttt cgattccttt ccggcgtaat
    13561 cttactttca attggtccaa aaactcaccc ttccttctct cataaaatgg tataggaaaa
    13621 aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa tagaataaaa agaagaccta
    13681 gaaatgattg gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt ttttgtctga
    13741 taaaaagtgc atacagaaaa tatggcgcac caaaaagggc aatcaaaatt cctgtaggta
    13801 cttccccact tagtagaatc actctagcaa ccatatctgc actaagcaga aaaatggaac
    13861 cgaacaaacc gctcagaaca aaggtccagt tattttcttt acgtacgact agcttcgcag
    13921 catgaggagc tagtagtcct acaaaagata aactacctgt caccgctaca ctaccgcttg
    13981 ccaaagcaac agctaacaac aaaaggattt tttgactttt agccacgtta aggcctaatc
    14041 cttgcgcgcg ttcttgacca aatgatagca atacaatcct ctgtgcataa agtcctccta
    14101 gcggaattaa gataaccgcc catggaagca atgcccaaac ataaggccag ctcgcacccc
    14161 agattgatcc cgctaaccat gcattcacaa atccataatt ttccttagat aattggatcg
    14221 tgcatagcaa tgttaatgca gaaaggccgg cattaacggc aataccggac aataagattc
    14281 gattcatacc caaaaagcca tttcgatcat aggccgcaag atagacacag caagcagcta
    14341 aaacaccacc tagaaaggct acaactagca atagaaacgg cagcgaatag tgcgaaaaga
    14401 aacctaaata aagcatcact aaaaatcctg ctccggcatt aatgcccaat atactagaat
    14461 ctgccaattc atttcttgtt actccttgca agatataacc agaaacgcca agacaagctc
    14521 ctgctaataa actgatcagt aatcgaggaa ctcgaaattc tctaacaatc agcgccaaag
    14581 aagatgattc tttccctatg aacgtggcaa aaagctgatc caggtaaata aaaggcgtac
    14641 caatcattaa actacctata gacaggatga caagtacaaa aagcaagaaa tagaacgtcc
    14701 aatatatttt tttcaccgtt gttccctcct tacttgtatc agtaaaaacg gaacgccaat
    14761 caatgcaatg actaatccaa aaggtgtttc aaaaggggga ttgatcatcc tcgctgtaaa
    14821 atccgccaac ataaccaatc caccgccaac taaaaaagag acaggtagaa cataacgata
    14881 gtccgttcca accattccgc gaacaactgg cggaaccaac agcccaataa atgaaacagg
    14941 tccgactaac gaaacggcaa tggctgctaa cactgcaact agacagaaaa caatcaaccg
    15001 cgttcgttta ggatgacttc caagagctaa tgcatgtgtt tcactcaaat gaatcaaaga
    15061 tattgctttt cctaatgaaa aagaaaccaa aacagcaagt atatataaag gaaacaatac
    15121 tgctaattgt ttccatgtga tattggcagc tccgcctaca aaccaaaaag caagatcctg
    15181 ctgcagatta aagaattggg taagtgcttg gctgatagca gtaaaaaaag aactaatagc
    15241 tacgccagct aaaatcagct gtaaaggatt catgcccatt ttcgaaaact gcaaaaaacc
    15301 aaatacgagc aaagttacag caaaagaacc tgccaatgac attagaaaca cagaaaaatc
    15361 agaagcttta ggccaaaaag caaaactaag ggccattgcc aacgaagcac ccgcgttgat
    15421 tcctagtaag cctgaatctg ctaaaggatt tcttgtcact ccttgcatca atgcaccgct
    15481 caatgcaaaa caactcccta caagaaatgc tcccgccaca cgaggcaaac gaatatttcg
    15541 tatgatttgg tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc aaaaatctgc
    15601 ttttcccaca gtcgctgcgc catttgaaag agcaacaaaa aaaataatca aaagaacaaa
    15661 acagaccaaa agcaggagtc tatttttctt catagtcctc ataattgatc ctcctgctct
    15721 tttggcaaca aatcataggt tagaaaaact ggtttatttg ttcttggaag cgtgacaatc
    15781 gttgcatcaa tctcaaatat ttttttcata cactcttttg tgatcacttc ttcaggtgta
    15841 ccttctgtga tcaacttacc gtttttcatt gctaataaat aatctgaaaa acgtgaagca
    15901 tgattaatgt catgaatcga catcaagatc gttgtttgtt tcatttgatt gatttcagtc
    15961 aataactgta ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg ttcatccaag
    16021 atcaagatat ctgtttcctg agcaagcgcc atagcgatcc agacacgctg ggtttgtcca
    16081 ccagacagcg ttgcgatatc ccgcatacga aatccagcta gacctgtagc atccaacgcc
    16141 cactcgattg cttcatgatc ttgcgtgtcc atgccactga acagtttttg ataagggtat
    16201 cgtccgtaag atactacttc ttctactgtc atacctgtag ggtgttcact agtttgtgct
    16261 aataatgcaa ttttttttgc aagttctttt gaactgtatg tagagatatc agagttattg
    16321 acaatgatct ttcctgattt cacaggtagt atacgggtaa aacttttcaa taaagtagat
    16381 tttccacttc catttggccc aatcaaacta gtgattttcc cttcaggaat cgtaacagac
    16441 atgttttcaa tgattttttt gttttcgtag ccggctgcaa tgtcagctgc tcttaatctc
    16501 gtcatttcac ccatcctttc gctgaaaatc attctcaatt atgacgctgt ttcaacattt
    16561 cgtcaagcgc tactttcata tctatagcca aatgaagcgg ttgacagcgt tcgaaatatc
    16621 aggaataata accaatgaga aagattctca attatgttag gaggaaagaa atgaaattaa
    16681 aaacaaaagt attcacatta tctagcatat tgctgctggc tggagtactg agcgcttgct
    16741 ccccctcttc agataaagaa acagctacaa ctgccaattc gacgacttca tccaatagta
    16801 ctcatacagt cacagataca cttggacata aagtagagat tccaaacgcg cccaaacgga
    16861 tcatcggcag ttacttagaa gactatctga ttgcactagg ggaaaaacct atcgcccagt
    16921 ggactgttgg cagtggttcc attcaagatt atctgcaaga tgatttaaag gatgtcccaa
    16981 cgatttctta tgaccttcct tatgagaaag tgttaagctt tgagcctgat ttgctgttga
    17041 tttcttcttc cgctactgtc gaaggcggga aatacgaaca atatagcaaa atcgcgccaa
    17101 cttatgttgt aaaaaacgga acagatgtca catgggaaga acaactaaaa gatgtcggca
    17161 aagctttggc aaaagaaaaa gaggcagaaa agatcatttc cgattatcaa aaatccatca
    17221 agaaaactcg agaagaacta gcagataaaa tcgagaacaa gacagctgca gtgttgtggg
    17281 tgaccaacaa ctcagccttt atggtcagca aaaatcggtc aagtggaaga atcgtatacg
    17341 acgatctgga atttggtgtt cctgatctag tggaagaggt ctccaaaaaa gccacttctg
    17401 actggtctgc tgtttcctct gaaaagctag ctgaattaga tgctgactac atcatccttg
    17461 tgaacagtga caaagatgct gccatgttca atgaaccaag ctggaagaat cttaaagcag
    17521 tgacagacaa tcatgtactg caattcggac ctgaatctag ttggctatat aacggaccta
    17581 ttgccagtca ggaaatggtc aatgatatca aagaaaaact aaattgattt taactaaaaa
    17641 aagcatcttc acgattagtc gtgatatgct ttttttctta ttaacaaact agacgcatag
    17701 attttattct tcttttgaag caaccctatt ttttactctt tcccattttt gaaaacgatg
    17761 ggcatataga ttttcttgat ccgttatccc ctcatactcc tctataaggg cgaagtctga
    17821 aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca atgttcgcca
    17881 gatccgacca gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg aaacatagat
    17941 atcttctgag atcgtttgac tatacgacaa aagttcatcg atcgaatgca ttacttccgc
    18001 attttctttc gtgcgaaaat ttggctgcgt tgtcaacacg atgatatggc gataaggaag
    18061 tgaaagatcc cccattcctt cataggtctt tcttcccatc actaaaattc gatccattgt
    18121 atgttcgcga aagaaacgca tatcatttgg caatcgccat ggtaacaaac catttttgcc
    18181 gatcagacca tttttatctt gtgcccacat ggaaataaac atattatccg ctccttatga
    18241 ttttagatgt tttgagataa aaaaagtctc aattctttga ccagcaagga attgagaact
    18301 ttaaaaacat tattttgttt cacggtgcaa agtaactttg cgttcgcgtg ggcaatattt
    18361 ttgtttttcc aaacgatcag gattgttacg tttgtttttg cttgttagat agttacgttc
    18421 tttacaagaa gtacactcta aagtgatgtt tacgcgcatg tttttccctc ccatttttac
    18481 aagaatcctc taagatgact ctcagacttg attatcttac catgattttt tctagattgc
    18541 tagtgttttt cacgattttg ttaagtaaaa ctacttgacg ataaatacaa cggttttact
    18601 tctcaaacaa aatctttttc tttttgtata aataccctta cacttgttta tatagattat
    18661 gttttttaat aaagggggat actaatatgc catggaacat gaaggacttt cctgcatcaa
    18721 tgaaaaactt agataaattg acaagaaaaa aagcaatcga catcgcaaat gcattattag
    18781 atgaagggta ccctgataac cgtgcgatcc caattgccat tgatcaagca aaaaaatggt
    18841 atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat ccaagcaaga
    18901 cggatgaaca cgatacgaat cctcgtgctg gaaaactgct tgattcagat gtgatcgtag
    18961 aatatgagga agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca agcaaccatt
    19021 ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac aaagaatcaa
    19081 ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga aatcgattat tgaacatatg
    19141 taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca atggtgttcc
    19201 tctgccaact ccgaaccata agtcataata tctataaaac gggaagagct gttttttgaa
    19261 attgtttctt atcactcagt gttaaacggt aggagaaaac ctctttttta ttgatatttg
    19321 attttcatca aacagtcaca tagtcgattg cttctgcttg cggaatcgtt tcatctacaa
    19381 agtagacttt ataccatgtc aagaaactga agattgtcca gatttttctt tgctcttctt
    19441 tacgtccttc gtgatggtca tctagcaact tcatgatttt ttcttgatca aaaaattctt
    19501 ttgcaaaatc tgcttcgaac aatgctttga cttgttcgta gccgtgttct tctttcaacc
    19561 aagctttgat tggcacaggg aatcctaatt tcacacgatt cgaccatttt tccggaagat
    19621 gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat aaaaacttac
    19681 ttggtatttg ttgagccaat ttcatgactt cgatatccaa taaaggtact cggacttcca
    19741 aagaacttgc catagaaagt ttatctgcct ttaacaagat atcttttggc atccattgat
    19801 gaagatcgac atattgcatc ttattgactt catctttgat tccttctgtt ttctcgtaat
    19861 gaacagtcat gatttcatcc acagatggtg ctgtttgata ttttggtgtt aacacttctg
    19921 atgcttctga ttcttcgaac actcgagcat gaccgataaa atattctttt gcaggagcaa
    19981 gtgattcata taaatggatc cgtccatgga aatttttcat ttttccgatt ttacgtccta
    20041 aattatagcg tgttcctttt ggtaattttt tcaatccttg agcaatcaca cggatgaatt
    20101 ttgaattcgt atggaatcca tacgtttggt atcccgcaaa cagttcatcc gccccttctc
    20161 cagattggac tacccgcaca ctttgtgaag caagatttgc taaaaagtac aaaggtacgc
    20221 aagaaggatt tgaatcaggt tcatctaagt gatactggat caaagggaat gctttgaacg
    20281 acatgtctcc gtcgatcact gctgctgtat tatctaagtc caatagttcg gttagttttc
    20341 tggcttcaat cgcttcatta taggttttat catcaaaccc gattgagaaa gaatgatctg
    20401 gtttcaatac agaggttaca taactggaat caaccccgct ggataagaaa gaaccaactt
    20461 ctacatcact gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg attttttcga
    20521 tccattcttg tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa tattgctgaa
    20581 tgtctatttt tccatcttta tacgtataat aatggccttc aggcaaacga tagacatctt
    20641 tgaagaatgt ttcgccgttt aatggagaat attggaaagt catataaggt ttcaacgctt
    20701 ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa acttttgatt tctgaaccaa
    20761 acataaatgt tccgttcatt tctgcataat aatatggttt gatcccaaag tggtctcttg
    20821 caccgaataa ctcattcttt tcattatccc aaatagcaaa agcaaacatt ccgcggatct
    20881 tttgcaataa ttctgttccc cattcttcgt aaccatgcaa aaggacttct gtatctgcat
    20941 gtgtttgaaa aacgtggcca gctgcaatca attcttctct caaaggctgg taattataga
    21001 tttcgccatt aaaaataatg atctttgtcc gatcttcatt gtaaataggc tgagtaccgc
    21061 cctctaagtc aataatactt aatcttctga atcctaaagc aacatgttta tcgatatatt
    21121 ctcctgaact attaggtcca cggtgaacga ttcgatccat cattgtatta ataattgttt
    21181 ttttgttgtc cttgtcgtta acaaaaccta caataccgca catttaactt tcacatccta
    21241 atataaaatt actgtttatt tcattcatat gcatcttatc atagaataaa tagcggacga
    21301 atattttctc tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt ctgtttttac
    21361 agaaaataaa gtcccaatat ctaatttacc atgttttctt ttttgaggct accttattct
    21421 cttcgtgtta aaaaaatatt agtggatgct atttccaaaa aaaccagcaa aaaaagacac
    21481 gacctaaaga tcgtgtctaa ttatgactgt atcagaaaat atgataccaa acggatctat
    21541 tcattaatta attgacatag tttatctgtt gctcttttat gactggataa ctgaccaatt
    21601 catgacgatc caagtcttct ttattcatat ttacagcagt aatttgactg tcttcatacg
    21661 tacgataata ataaatccct ttatcaacgt tacagcaaga agaataaatc gtatattcgt
    21721 attttctttc tccagtatcg catactcctt tttgttgctc gactgagcct agtatatgaa
    21781 aaaattgact gatactttca gattctgaat cacctgatgc agcattcatt ttagtaaatg
    21841 tggcttttac aaatcgagaa acagaagaga gatctccagg taagcccaat cctcccatcc
    21901 cacgactata aacattcaag ctgatctggt ttgaaaaatg attttctggt gttccatttg
    21961 atagcgagcg ataattgttc agattaaata attgataatc aaatggcgga ttatttgtca
    22021 acacaccgac aggattatca tataaatgaa gtccatcttt catactttca attactatcg
    22081 atttttcttt atcagctaat aaccagtgta aaggtgacaa gggtagtttt tcactaaaat
    22141 cgatattgac tagattgata gtagccaata attctttagc ttcgtctatg gttgagcatt
    22201 gccctagtat ccatggaata aactcaaatg gtgcaacgtt atcttttcct tcttgtaatt
    22261 ctttataatc agcattgcca ggaaaattca gtcctgccat actcaaccct ttttcatttg
    22321 tagcctcata atagagtgga tagttggata ccccagctgc tattcctatc attgcataat
    22381 gtttatctaa attttttacc tttctaaaat cgaaacgata atttcttgga gtaatagtga
    22441 ccacttcatt ataagacatc tcataatcaa aattccttcc aaaataatgg tctttcgttg
    22501 cataagtgat tgcggtacac atcgtttccc ctcctaaaac aaatcgtttt tacactttta
    22561 tcttacatct agtatttttt ttatcaaaaa aaggccaaag aagattgatt tactagcatt
    22621 tataaaaaat agtactcagg gggaatcaaa aaatattgta ggttaaaagt acaacaa
//
LOCUS       DAZKMK010000042        22422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000042 DAZKMK010000000
VERSION     DAZKMK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.042"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8V1_001524"
     CDS             6..305
                     /locus_tag="ACG8V1_001524"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0329620.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8V1_001525"
     CDS             378..1739
                     /locus_tag="ACG8V1_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0329621.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8V1_001526"
     CDS             1761..2021
                     /locus_tag="ACG8V1_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329622.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8V1_001527"
     CDS             2035..2787
                     /locus_tag="ACG8V1_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0329623.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8V1_001528"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8V1_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0329624.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8V1_001529"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8V1_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0329625.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8V1_001530"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8V1_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0329626.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8V1_001531"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8V1_001531"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0329627.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8V1_001532"
     CDS             7161..8558
                     /locus_tag="ACG8V1_001532"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0329628.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8V1_001533"
     CDS             8639..9079
                     /locus_tag="ACG8V1_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0329629.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8V1_001534"
     CDS             9227..9553
                     /locus_tag="ACG8V1_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0329630.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8V1_001535"
     CDS             9554..10303
                     /locus_tag="ACG8V1_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0329631.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8V1_001536"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8V1_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0329632.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8V1_001537"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8V1_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329633.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8V1_001538"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8V1_001538"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0329634.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8V1_001539"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8V1_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0329635.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG8V1_001540"
     CDS             14770..17436
                     /locus_tag="ACG8V1_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0329636.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG8V1_001541"
     CDS             17800..18552
                     /locus_tag="ACG8V1_001541"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0329637.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACG8V1_001542"
     CDS             complement(18574..19728)
                     /locus_tag="ACG8V1_001542"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0329638.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="ACG8V1_001543"
     CDS             19913..22354
                     /locus_tag="ACG8V1_001543"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0329639.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7198 a         3933 c         4673 g         6618 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt tt
//
LOCUS       DAZKMK010000043        22414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000043 DAZKMK010000000
VERSION     DAZKMK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.043"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..197)
                     /locus_tag="ACG8V1_001544"
     CDS             complement(<1..197)
                     /locus_tag="ACG8V1_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329640.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITML"
     gene            complement(288..740)
                     /locus_tag="ACG8V1_001545"
     CDS             complement(288..740)
                     /locus_tag="ACG8V1_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0329641.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(753..1163)
                     /locus_tag="ACG8V1_001546"
     CDS             complement(753..1163)
                     /locus_tag="ACG8V1_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0329642.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1438..1650
                     /locus_tag="ACG8V1_001547"
     CDS             1438..1650
                     /locus_tag="ACG8V1_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0329643.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1653..1865
                     /locus_tag="ACG8V1_001548"
     CDS             1653..1865
                     /locus_tag="ACG8V1_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329644.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2005..2160
                     /locus_tag="ACG8V1_001549"
     CDS             2005..2160
                     /locus_tag="ACG8V1_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329645.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2157..2381)
                     /locus_tag="ACG8V1_001550"
     CDS             complement(2157..2381)
                     /locus_tag="ACG8V1_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0329646.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2459..2647
                     /locus_tag="ACG8V1_001551"
     CDS             2459..2647
                     /locus_tag="ACG8V1_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329647.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2644..2817
                     /locus_tag="ACG8V1_001552"
     CDS             2644..2817
                     /locus_tag="ACG8V1_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329648.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2786..2914
                     /locus_tag="ACG8V1_001553"
     CDS             2786..2914
                     /locus_tag="ACG8V1_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329649.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2973..3308
                     /locus_tag="ACG8V1_001554"
     CDS             2973..3308
                     /locus_tag="ACG8V1_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329650.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3541..4482
                     /locus_tag="ACG8V1_001555"
     CDS             3541..4482
                     /locus_tag="ACG8V1_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0329651.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4484..5374
                     /locus_tag="ACG8V1_001556"
     CDS             4484..5374
                     /locus_tag="ACG8V1_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0329652.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5417..6217
                     /locus_tag="ACG8V1_001557"
     CDS             5417..6217
                     /locus_tag="ACG8V1_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0329653.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6232..7083
                     /locus_tag="ACG8V1_001558"
     CDS             6232..7083
                     /locus_tag="ACG8V1_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0329654.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7284..7745
                     /locus_tag="ACG8V1_001559"
     CDS             7284..7745
                     /locus_tag="ACG8V1_001559"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0329655.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7771..8223
                     /locus_tag="ACG8V1_001560"
     CDS             7771..8223
                     /locus_tag="ACG8V1_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329656.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8329..8523
                     /locus_tag="ACG8V1_001561"
     CDS             8329..8523
                     /locus_tag="ACG8V1_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329657.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8554..8847
                     /locus_tag="ACG8V1_001562"
     CDS             8554..8847
                     /locus_tag="ACG8V1_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329658.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8778..9317
                     /locus_tag="ACG8V1_001563"
     CDS             8778..9317
                     /locus_tag="ACG8V1_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0329659.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9392..9859
                     /locus_tag="ACG8V1_001564"
     CDS             9392..9859
                     /locus_tag="ACG8V1_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0329660.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10606..10836
                     /locus_tag="ACG8V1_001565"
     CDS             10606..10836
                     /locus_tag="ACG8V1_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329661.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10995..11828
                     /gene="terS"
                     /locus_tag="ACG8V1_001566"
     CDS             10995..11828
                     /gene="terS"
                     /locus_tag="ACG8V1_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0329662.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11821..13236
                     /locus_tag="ACG8V1_001567"
     CDS             11821..13236
                     /locus_tag="ACG8V1_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0329663.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13248..14777
                     /locus_tag="ACG8V1_001568"
     CDS             13248..14777
                     /locus_tag="ACG8V1_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0329664.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14695..15747
                     /locus_tag="ACG8V1_001569"
     CDS             14695..15747
                     /locus_tag="ACG8V1_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0329665.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15749..16066
                     /locus_tag="ACG8V1_001570"
     CDS             15749..16066
                     /locus_tag="ACG8V1_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329666.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16193..16816
                     /locus_tag="ACG8V1_001571"
     CDS             16193..16816
                     /locus_tag="ACG8V1_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0329667.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16831..17721
                     /locus_tag="ACG8V1_001572"
     CDS             16831..17721
                     /locus_tag="ACG8V1_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0329668.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17744..17959
                     /locus_tag="ACG8V1_001573"
     CDS             17744..17959
                     /locus_tag="ACG8V1_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329669.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17971..18312
                     /locus_tag="ACG8V1_001574"
     CDS             17971..18312
                     /locus_tag="ACG8V1_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329670.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18312..18683
                     /locus_tag="ACG8V1_001575"
     CDS             18312..18683
                     /locus_tag="ACG8V1_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329671.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18676..19071
                     /locus_tag="ACG8V1_001576"
     CDS             18676..19071
                     /locus_tag="ACG8V1_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0329672.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19073..19450
                     /locus_tag="ACG8V1_001577"
     CDS             19073..19450
                     /locus_tag="ACG8V1_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0329673.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19451..20059
                     /locus_tag="ACG8V1_001578"
     CDS             19451..20059
                     /locus_tag="ACG8V1_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0329674.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20059..20400
                     /locus_tag="ACG8V1_001579"
     CDS             20059..20400
                     /locus_tag="ACG8V1_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329675.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20642..>22414
                     /locus_tag="ACG8V1_001580"
     CDS             20642..>22414
                     /locus_tag="ACG8V1_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0329676.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAV"
BASE COUNT         8033 a         3299 c         4596 g         6486 t
ORIGIN      
        1 tttaacatag taatttgttt atttaaatct gatagtgttt cttccttact taatataaat
       61 ctttctctat catgtaattt atttagtttt tcatcatttt tttgttctaa ttcataagct
      121 tcttttttta ttgtgttagc tttcgacagt ttagtgattc ctataattaa taaaataagt
      181 ccaatcaata ataacattca aaactcctca tttctttgat ataattattg tagagtttac
      241 tcagaaatga ggaaagagtc catgtgaaag catgggcttt tttattttta atgttgtaat
      301 actaaatgtt caagattatg aggaagccct aaatattcaa taatttggaa ctgactcata
      361 tcagataaat cttgttcatc tattgttcta gttaacattt caattgcaaa catattagct
      421 tcagcttcta taccataaat catacaacta aggttgtttg ctctaaaagc tgctgtgcaa
      481 atacctttat ggagtctaac gtgggctatt tcatgccata atacaaatct tttcatagaa
      541 ggtgataggt tttgattaat taatatcaca taacatcttt tattgtgtat cgtttgtcct
      601 aatgttttat tccctaaatc agtttcaatt aaatctatat cagaatattt gattatttta
      661 tatggattaa tcgcatcaga tccaaactct acaaataatt tttctatttc ttctaatatc
      721 cagtatttgt ttaaatgcat accaaagccc acctattgac gatatttttt aggagtgaat
      781 tttttctttg ctttaacttt ggttaaggca agtgcaagtt gaatggcatc gtataatttt
      841 tccttatctt catcgtctaa aggttcacca taataatttg tttcagcatc tgaattcaag
      901 ccttcaagta atttttcagc ttgtaaagca atatcttgtt tatctttttc tgttaaatca
      961 taataatggc gtttatcagt acgtcctaaa agataatcag ttgatacatt aaagtaatct
     1021 gcaacttttt gtattttatc tccagatgga gtacttttat tccatctaga catgcttcca
     1081 tttccgaaat ttaactttct ttcaagttct gccaacgtca ttgaacgttc ttttaataag
     1141 atttttatac ggtctaatag cataggatat gcattccttt caaaataccg acaaaaaaag
     1201 tatttttctg caaattatct atttacaaat agagaatttg cagttatact agttttgtaa
     1261 gcaagatatt tacggcatac aaagactaaa agcaataaac taatagtctc cccagacatc
     1321 agtaagctcg ctttttgtgc gctgtatttt gtatgcttac ataatagcat atttgcagtt
     1381 tttgtcaaac ttattagaca aaaatattag aaaatattcg ataagggggt atgaagcatg
     1441 actttaaaac agcaagtatt aattgcttta gtaaagaaag gttggagtca aagagagtta
     1501 gctcgacgaa tgggtatatc aattacttat ttacgagata tcttcatcga aaaaagaaaa
     1561 cctaaggaac gtttaaaaca aatcgaagaa cttttggata tcaagttaga agtagattct
     1621 aatgatcagc catcagaaca ggaggcgtaa aaatggtaca acgtttgagc aaagcggagt
     1681 ttaaagagaa atataagtat tctgaaagta cttatcaaag aagaatgaag gaatttaaaa
     1741 aatcacgttt tagtgagggg tataacgcag ttacttccaa agaaataaca attgatgtcg
     1801 atttatatga acagtttaga acatggcgtt ctaaaaatcg atttagagtt agagctgttt
     1861 gttaatagct aaaaaatcta aattcaccac ttctgaaaag aggagttgct gctctccttt
     1921 caagtttgcc aaagtccacg ttttttcatt ttggcaactc ctcttcttgg aggtggtgac
     1981 ggcgagagac gaaggaggga tgacatgaag gaagaatcaa aaatagggat tttgaatgag
     2041 aacaattaca caagattaat tattgaaaca gaagaaggga aaaaagtagc agaagttaca
     2101 ttgaccgatg caactcctgc tgacggttat cgtattcgat taactcctaa atatgattaa
     2161 ccttttggtg ggtgtggatc gtttccgtga ctatcacggc tttgaatttt tccatctctg
     2221 ccatgaataa atagctcaga tccttgattt ttagaaatct ctctagcgat ttttactgct
     2281 tctgattttg tattagtatg tgcagtcgct ttagaatttc cagcaccttt gacattccaa
     2341 ccaccattgg aattaggtac aacatgttga ttttttccca tagttttcac ctccatttct
     2401 atagaaatat tttatcaaat agaaatggag aaattcaata aacgaaagga gaagtgatat
     2461 ggcatatacc ttgcaacaag aacatcaaat tctcggtttg attaaacaac gcaggaaaca
     2521 attacaagat gaccgtgcag cgcttagaaa agccgatgag ctatcagata ggcaagctga
     2581 actaattgct tctgaacttg aggatttgag aatgctagaa ataaaaaata gggagattag
     2641 attatgaaga agacagacac actttttata ggattcattt tggggttatt agtgattgta
     2701 gcgcaccaaa gtattatcgg gggaagcttg ttcgcagcat tgatggtttt aatcaatctg
     2761 cttgattcaa aagaaaggag caactatggc acgagaagaa gcgctaaaaa tcggtaaagt
     2821 gattgctgat aatcggtggg gaaatagccg ccctattatt ttaagcaagc aacatatcga
     2881 aaagcaaaaa gcatggcaac aaataaaaaa gtgactctgc cagccagcaa agagtcacat
     2941 acaaatttat aaaactagga gaattatatc acatgaataa gaaaattgaa aatcttatcg
     3001 aagaattgaa acgtgaatgc caaaagcaag gtgtttctat tatttgtaca gcccaaaaag
     3061 aaggggaact taaaagtctc gtttatggtg agacaacaga aattttactt tgtcttgcta
     3121 tgcaagaaga acatttagat gaaaatctcc cattgtcagc acatattatg cgtagaattg
     3181 ctgtggatgc ttacgaacaa gcgaaaaatg aagaagaaaa tcaaccttca aatcatactt
     3241 ttgttattaa taacaaggaa gatttagctg atgtgatgac tcgtattctc aagggggaat
     3301 ttcaatgatg aatgaaaaaa caattgatct actcattatt ttatttttca cgttagtgat
     3361 tgtagcgaaa agttatgtaa tctttggctt gttattcgca attaccatgc ttgtgctttg
     3421 cgtgttagca agcagaaaag aggttaaaca tgaacgagtt agcaaatctt gacaactatt
     3481 taactgatcc tgattatgct aaaccaccat atgaagcacc aattgatgag gaggatgaag
     3541 atgagtaaat ctaccttaga aatgagccat caagaatggc ttgaagaccg taagaaaggc
     3601 atcggaggtt cggatgttgg aacaatttta ggattgaata aatggaaatc tccttatcaa
     3661 ctatggctag aaaaaacagg acaagtagta cttgaagaat caggaagtga gcccgcttat
     3721 tggggtaatg ttttagaaga agtggttgct aaagagtttc aggaacgtac aggcaaaaaa
     3781 gtacgcagaa gaaaccaagt atttgaacat ccattacatc catttttaag agcaaatatt
     3841 gatcgggaag tagtgggaga aaatgccatt ctggaatgca aaacagccaa tcaatttctc
     3901 ggtaaagaat gggaaggcga agaggtaccg cttagctatc tctgtcaagt tcaacattac
     3961 atgaacgttc taaacaaaga ctattgttac atcgctgtct taatcggtgg tcaaaaattt
     4021 atctggaagc ggattgaacg agatcaagag ctgatcgata caatcactga acaattagta
     4081 gagttttggg aaacaaacgt tcttggaggt atcgagccaa ttattgatgg tagtcaagcg
     4141 acagctgatt tcttgaaaga gaagtatgca gatgttgaag acgttcaaac gacgttacca
     4201 attcatttcg atgaactagt tgaacagaaa aacgaactca aacggactaa gaaagaaatt
     4261 gaatcagcta tccgtcaagt agacaacgag attatcagcg agctaggtaa acgtgaagcc
     4321 agtatcggta tcactcaaaa aaacatcatc agttggaaat ttgtccgtac gagacgtatg
     4381 aactcgaaga aactagcaga gaaatatcca gatgtcgcaa atgatgaaga gatttataac
     4441 gttactgaat caagaaggct aaccgaaagg gagatcaaat aatatggcaa caaatgaatc
     4501 gttaaaaaat caattggcag caaagccaca gaaacaagtt gcaccaggtc aattaggact
     4561 taaagctttg atgaatacac caacaatgag aaagaagttc gaagaggtgc ttcatgacaa
     4621 tgctaatgct tttatgtcga atgttatgac tcttgtatct aatgacagtt atcttgcaga
     4681 tagtgaaccg atgtctatca tgagtggtgc gttaactgct gcaacattaa atcttgggct
     4741 agataagaat ttaggttatg catatttagt tccattcaat agtaaaaaca agcaaacagg
     4801 aaaatgggaa aagaaagctc aattcatgct tggctataaa ggatatatcc aattagccca
     4861 acgatcaggt aaatacaaag cattaaatgt gatcgaagtt tacgaaggag aactaaaaag
     4921 ctggaaccga ctgacagaag agtttgagtt tgatccaaat ggtagaacgt ctgatgaagt
     4981 cattggatat gttggctatt tcgagttact gaatggattc aaaaaaactg tctattggac
     5041 caaacaagaa atcgaagctc atcggattgc taacaataaa gatcgcgata agacaaagtt
     5101 aagtggtgtg tgggcatctg attacaatgc aatggcacga aaaactgttt tgagaaatct
     5161 tctttctaaa tggggaatct tgtccatcga aatgcaagaa gccaccacat cggatgagag
     5221 agtccaaagg gttcaagaag acggcagcat tattgctgaa acagaagttg aagaagatat
     5281 tcctgaaaga aaagaagcag aggttatatc tgaagaaaat gaagatgtac aaactggatt
     5341 atttgatgca tctaatccgc cgttaaacaa ataatgaggg agctttctcc cttgcttttc
     5401 tagaggagaa atacgaatga acacaggata tgtcaaatta catcgaaaag tgatgaattc
     5461 attcgtttgg accaatcctt atatgtataa attatggaac ttgtgtttga tgaaagcgag
     5521 tcatgagaat cgcaagatac tttttaatgg aaaagagata caagtgaaca gcggagaatt
     5581 cgtcacaggg cgcgatgcta tcacatctga gatgaacgaa ggcgtgaaac gtgaacatca
     5641 agtgaacagc ggttctgtat ggagatggct aaaacaattt gaaaaaaacg gaatgttgaa
     5701 catcaaatca actacgaaat acagcgttat atcaataaaa aactggtctt tgtaccaaag
     5761 cagtgaacaa caaatgaaca tcaaacgcac aacaagtgaa caacaagtgc acacaaacaa
     5821 gaatgaaaag aattataaga atgaaaagaa taataaaaca tcatcgtcgc aaccacgcaa
     5881 aaagcgtgtt tacgacaccg actcagttta ctacattctc gcggaggagt tattcaaaca
     5941 gatttgtcag aatcaggaaa tcaaaaagcc gaatctgcaa agttgggcag atgacattcg
     6001 gaaaatgatc gagatcgaca agcggaccga aaaccaggta cggggaatga ttgaatggag
     6061 tcagcacaat gtgttttggg catcgaatat tttatctgct aaaaaattgc gagaaaaata
     6121 cgacacaatg gcagcgcaag caaatcgtga ttataaaaca aaacaaacta aaacgcttga
     6181 atacgagaaa tttagcacag atgagttgcc tatttgagag gaggcgtagg catggaaact
     6241 gttggggaaa tcatggaaaa gctgatacag aaagtgcttg tccaacgtgg cgaatgtcct
     6301 gaatgcggac agcctttgta tggatggcgt acgaagaacc ctgatggctc agaacgttgt
     6361 aagccaacct gtatgcaatg tgggtataaa gcattgcgag ttcaggaaga cttacaaaca
     6421 gaacgaattt acaacgagag cctgaaagca agagcaatca attttttcaa aggtggttct
     6481 gttgtgccta atcaagcgtt gtttgattgc acattgcaga attatcaaat tgtcgatcaa
     6541 gaaacaagac aagcggttga agtaaccaaa cgctttgtta attcagtctt gttaggaaat
     6601 ccaagtcacc ttgttttaac tggaaaacaa ggaacaggta aaagtcacct agcgatggca
     6661 gcggcttggg aagtcttgaa gcgatcaaac tacgataaaa aaatcttatt tatcgggtta
     6721 caggaaatgc tggatcaaat caaattttcg tacaacaatc ctgaactcag aaaaacgatt
     6781 gagggatcgt tgattgcaga tatcaaaaca gcggatttag tcatcattga tgatatcggt
     6841 tcagaactag gaaaagatgc atcagatagt cgagcgtttg gcataaacac gctaaattcg
     6901 ttcttggatg cacgacagaa cctagcaacg attatcacaa cgaacttgct tggtgaagaa
     6961 ctgaaaaaag cttacggtac gagaacaata tcaagaatgt ttgtcaactc tgatggattt
     7021 acgatggtat tttctcaaac agcagacaag cgcataaaac cagtcaaagg agcgatcgca
     7081 tgaatttgac taaacaagaa aaaagaatct gtcgtcagat aatgttgcaa attataggtg
     7141 atcgtgacat ggacgaatta tctcaacaag agagtcgaat ggttcggaag ataagtaagc
     7201 taattattta tcgggataca gaaagatttc tcactgtttt aggtaacgtc tacgagaatc
     7261 cagagttatt ggaggtagag taattgccaa aaatattaga tgcatgttgc ggtagtagat
     7321 tgttctggtt tgataagaac aattctgacg taacatttat ggattgtcgt cagcagtatg
     7381 aagaattatc aacaggtcat gtgatcaatg ttgatccaga tgtggtcgca gactttcgcg
     7441 atatgccttt tgaagatgat gtgttcgata tggttgtatt tgacccgcca cacttaagac
     7501 atgtcggcga aaactcatgg cttgccaaaa aatatggaaa attagacgaa ctttggccag
     7561 aagacatcag gcaaggtttt gcagagtgta tgcgagtttt acgaccttca ggaacattaa
     7621 tttttaagtg gaacgaggaa cagataccac tttctgatgt gttagaggct attggagaac
     7681 agccattgtt tggtaataaa cgaagcaaaa cacattggct tgtgtttatg aaaaaattca
     7741 gctaacggag gaaacaaagg aggaaaagta atgaagattg tattaaataa atgttttggt
     7801 ggctttgggt tgagccatga agcaaaaatg gaaattttta aaagaaaaaa tattgaagta
     7861 ttcccataca tcaataactt tagctatgac tcagatgatg aatacacgag atacacaggc
     7921 caaaagctag gttcgatgga ttttatctac tatttcaaaa aagacccgaa aatcgacaaa
     7981 gttacaggaa tttatagcga aattgaccga ctgtacggaa ttgctgatga ttccagtttt
     8041 tcaagtgatt caaacagagg tgataaggac ctcgttgctg ttgtcgaaaa actaggaagt
     8101 gaggccagtg gaccatatgc aagtcttaaa gtcgttgata tacccgatgg ggctgagtgg
     8161 gaaataagtg attatgacgg cgttgaaact gcacattatg gttttcaaac aggtagtatt
     8221 tagtccacta atgacaacta aagaggaggc agaaggatga gtgaagatga atactttgaa
     8281 cagtagtcta ttaacgacag agatttttaa ctggaaaggg gtgtagtcat ggacgcatcg
     8341 gaagtagtaa ataaaattaa tagtctcaaa gaggttttcg gagacgtcga agtagtgata
     8401 aacgcacaag gctatgacaa cgaatttttt aagcagatta ccgacattag cattcagcaa
     8461 ggaatggaag acgaagacgg aaactttatc gatgaagtag ccattttagt gacttgcgag
     8521 taagtaattg acagctaaag aggaggaagc ggaatgaaac taaaagacgg attttactcc
     8581 agcggccacg gcatcggcgg tttaatgcta gatatgccga caaagaatcc taaaacacgt
     8641 aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg attcatctat
     8701 ccatttcgtg gatatgtaga acacgtctat aatcactcag caatcattcg cattgaaaac
     8761 acgatggaat gtgataagtg gttagcgaaa agcaaagaga atttagcagt ggtgagattg
     8821 gtggacatgg aagttataaa atcttaattt tgctttttta caactatcct acaacaatga
     8881 gcgttattcc acaactggaa ggagaagagg caattgtgaa cattttagag aatatagata
     8941 ttaagcaaac aaggaaaaat gctagacgat tactaaaaag atacagaaat ttagaacgtt
     9001 tggtcggacc agtgaaaata gacttttccg tgatgactgt taccaaaaat ttgaaattca
     9061 caattgacag tcaaaacgaa gaaatcattg aagcgataag tactcgagat tcggttatcg
     9121 aagcattaac acggctaagt agaatccatt tccaggtact ttattatagt tattgttttc
     9181 ctaataagat gtcaatgtat caaataggag aaaaattagg atattccgat agaactatcg
     9241 agagaatgaa ggcagtagct ttggttgaat ttgctgaggc gtataaatca ggagaactca
     9301 tttcacgtac aaaataaaaa aagtcggatt cctccgacta tgaataatat ttccgacata
     9361 agtattatat cataattggg ggaatcagag gatggtactt ttcgatgtaa agaaatatga
     9421 gacaccaagc gcgaaagatg ttgatatgga acgcacaaaa cataatgtcg ctgtgtttct
     9481 ttcagcatat ctatcagcta gatgtagagt agggcaacat cgagaaccaa aagtgacagc
     9541 atcttattcc ttggttccac cttctacagc tgatcatata tttgaagcag aaagaatgct
     9601 gatcgataaa gaagaagcac aggaagaatt tgagtatctg cataaattgt ttgttcgagg
     9661 ctattcagcg atacagcatc cgcataagcc tgatgtgact gaaaggcgca agaagatatt
     9721 ctatgatcgt tatatcaatg gtctgcccat ttatgtaact gctcaaagga ataatactag
     9781 cgaagaatcg gttaaagtag aatcaaacag aattatcatc caatttgctt catcgttaga
     9841 actggttgct ttcaagtagc cagtttttac actttttata cccttttatt accaatttgg
     9901 tttccatttt ataccttttt tataccaatc acttacctat tcaacgttgt attatgatag
     9961 tgtcgaaaaa ttaggaaaca ggatcgacaa aataaaatgt aagggaggaa atctccctca
    10021 tcgtttaatt aagcttcgat aggcagcaac ggaaatatta agaataagga tgtgaatttc
    10081 aactccttct aaattgttct tattatctat catccgttgc tgtctattgt ttttttaatt
    10141 attcacacga taactaaagg taagtgaaga gaaatgattc cattaataat ttcaattttt
    10201 gcgctttgtc ttaatgtata tatgattgga tttaaaaatg ggcaaaataa aaaatagtag
    10261 cagccaagaa taattttata gtgtcactgt ggcggaaagg gtagacgcta agcatgtgtg
    10321 ctaggtcaat gcttcggcaa ccatgcaagg ttcgattcct tgccagtgac tttggcaacc
    10381 gaggcatcgg cggtttaaaa atataggggt gcgcaatttc gtacgcgttt tgtgcatcgt
    10441 gtaggttgct attacataac tggttaggtt agattgagat ttgggattcg gtacaaatga
    10501 atcgtcaaat gtctcaagca caggatcgga aacgtccctg cctgtgcatt acatattaga
    10561 tcactctttg agtggtcttt ttctttacat aaaggaggct acataatgag aaactactgg
    10621 tatgtatcgc taactaatga atatcctcga accattgatg attgttcagt gcgtgttgtg
    10681 cgttctgtac aaatcaaagg gaagtactct attgtcgaaa tggggagaga agcagaacca
    10741 tgtgagattg atgcgtgcaa gctcgtttat tgcggtcatg gtttctatga tgaaccaaac
    10801 attcaaaata atattaacaa gaatttgagg gattagaatg caagaaatgg cttaaaaatc
    10861 tccaatcagg acaaatcaag ttagacaaag tttccgattt aaagatattg attgaagctg
    10921 atttaatgct aaaagatatt gaaaattaga aaacaaaact caacctaaac agaatgtgag
    10981 gtggtgtgta ttgaatggca agacaacgtg atccaagacg tgatgaagcc aaaagaattt
    11041 ggttagaatc caacggagaa aagcagttaa aggaaattgc atctgaatta aatgtttcag
    11101 attctcaggt tagaaaatgg aaatcgcaag acaaatggag cgctgaattg attagtaacg
    11161 ttaccaatgg caaaagtaac gttactaatc aaggtggcgc tcctattggt aatcaaaatg
    11221 ctaaaggtaa caaaggaaat agccgagctt ctccgccagt gggtaataaa aacgctttga
    11281 aaacaggtga gtatgaaaca atattttttg acacgttaac cgataatgag aaggacatct
    11341 attctagttt gaatgatgat ccttcttttg ctttgtccga agaaatacga cttcttaaaa
    11401 taagacaact tcgaatgatg aaaagaatcc aacaagctga agctggacta aatgatgaag
    11461 aagtcgaacg attgcagcag ctaagaaaga ttaaaactcc gattgaaaaa aatggtaaaa
    11521 agctagaaat caagcgtgag gttatgcaag atgtgcagat tagcagaaaa aaacatcgca
    11581 aaattgatga tattctttca attgaagatt cattgactcg gattagcaac cagttagcta
    11641 aagccatcaa gcaaatgaat gaactttata tgaatgaata cagaactgat ttaattaaag
    11701 ctcagactga taagatccaa gctgagacaa atgaaattgg cggaaataat tcaggtgaag
    11761 aaatagaaga atggaaacag gcagttttaa atgccgcaaa caaacgggcg gtgaaagaaa
    11821 atgaataatg aatttattcc ttttgctgat attggttctg ccattgatta ttactatgat
    11881 aaaccagtag ctttttgcca agatattttg catttgaatc ctgatgaatg gcaagaaaat
    11941 gttttaaatg atttagctga attttcaaag gtttctgttc gttctggtca aggagttgga
    12001 aaaacagcat tagaagcagg agcaatactt tggttcttaa cgtgtcgacc ctacgctaaa
    12061 gtaatagcaa cagctccgac aatgaagcaa ctttacgatg tactttgggc agaggtagct
    12121 aaatggttaa atgatagctt gatcaaaaac ttactgaagt ggacaaagac caaaatttat
    12181 atggttggtg attcagagcg ttggtttgct acggctagaa cagcgactaa accagaaagt
    12241 atgcaaggtt ttcacgagga ccatatgttg attgtggtag atgaagcttc tggtgtgtct
    12301 gatccaatta tggaagctat tcttggtacg ctatcaggtt ttgataataa gctgttgatg
    12361 tgtggaaacc ccaataatat tgaaggtgtc ttttatgatt cccataattc agaccgtgat
    12421 aaatacagag ttcataaagt atcaagctat gatagtaaac gtacaaacaa agacaatata
    12481 gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg ctcgtgtccg tatttttgga
    12541 gaatttccga aaggtgcgtt ggattcattt atcagtcttg aaacagttga attggctaca
    12601 gaaaaacaaa ttagtgattc tttagtcaat aaaacaaagg ttgctcatat tggtgtcgac
    12661 gtagctcgat atggtgatga ttctacgatt ctctttccta gaattgctac cagggcattg
    12721 gagtatgaga agtattcaaa acgtagcacc atggaaacaa caggatatgt catcaacatg
    12781 gccaagaatc taatgagtca atatccgagt attgataaag tgatgattaa agtcgatgac
    12841 actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac ttatagaaga caaacattat
    12901 ccttttgagg tgtttggagt gaataacggt tcaacatcag aagacgattt ttacgataat
    12961 ttaggtactc aactatgggg aaacatcaag gaaatgttag aagaaaatat gacagcaaat
    13021 cttaacggag aacagcctgt tattgaattg ccttctgata gttcgttaat caaagaatta
    13081 agtactcgca aattcaaaat gacaagtaga agtcgtatac gtttagaaag taaagatgat
    13141 atgaaaaagc gaaatattgg tagtcccgat attgctgacg cactggcttt agcgttttat
    13201 gagccaccaa gtcactatca atttattcaa ttttaggagg tgagcttttg attagtacag
    13261 tattatcttt agagagatat aagaagctac gtgttaaata tgcaacacaa attgaagatg
    13321 gaatgtttga tccgaatggt tttatagaag atatgaaacc attttttgct gatcgcgaaa
    13381 gaaaatatct agcttatact agcgaaaaga atgaaataga taatagacca aagcctaaca
    13441 ctgatattgt aaaagttaat aataaacttc atgctggtat gtattcaatt gtcgtagacc
    13501 aagcagtcaa tcattttact ggtataccta tcaaatggga ttacgatgta tcggaacaaa
    13561 aaagaacact cattcaaaga ttaaaagata aattcttaaa aaatgatata gaacttccga
    13621 cagttccaga agcttttaac aaattaacaa gcaatcttga ttccatgaga ttcgcaatgc
    13681 ttgattctga aactgcaact tttcaaggag cttgtggagt agctttccga ttgctagagc
    13741 ctgtggaaga agatgatggt tggaagttaa gagcaagtaa tattgaacct tggagagcag
    13801 aaagatatgg aaatgccgga atctatatca aagaaaagta tgactcttac cagaaaaaat
    13861 tttttcaaga aatgaaagtt atgacaagaa ataaaattct gacatatgct tgctatggtg
    13921 atttgaattt tgttacaagc ggaacgttca aaaaaattga tgagaccgat aaccctttgg
    13981 ggacgattac cttggcagaa tttaaaaata atacgaatcg ttattgtgat tttgaagtag
    14041 ctgaggaaat tggagatgca attgatcgtg ctttgtctga tcaacaaaac gaaatcgaac
    14101 aatttaagct tgcgtacatg cttgttactg gcacaacaat gagcaaaggc actgctaaag
    14161 aaatgatgaa tcagctaggg attatcaact taaaagcccc tactgcaaaa gctgaatatg
    14221 tcacaaaaaa tttagcaaaa gatttcaatg aatatcatat ggatctattg aaaaaacagt
    14281 tttacaccat ttgtaaagca atcgatttta acgatgaggt gtttaaatca aacagttctg
    14341 gagaagctcg caagtggcaa atcattagcc tagaagcgaa aacaaatact aaagagcaat
    14401 attttagaga aggattgaaa gaatgcgcag aaacaattgc tgcttttctt aagtttcatg
    14461 acaaagtaga aattgagcct gaaaaaatta tttttacttt ctctagatct ttgccaactg
    14521 atttaagtta tttagctgaa gctttaccta aacttgcacc atacgtatcc aaacgaacta
    14581 ttcagagcca aattccattt gtaacagatg ttgattatga aaatgaaatg atggaattgg
    14641 aaagtggaag tgcttatcca gatagtgaat acaattttgg cggaggtggc aatagtgacg
    14701 atagaaacga agtattggac caaacgtcga gaactggagg atcaagcaag gctcaaacaa
    14761 gaaaatcaga cacttaaaaa attaactagt gtatttcctg aagcactgaa agagatacaa
    14821 gcaaaactat tatcacaagc tgacttacac aatatcactt atccagaaat gatggagttt
    14881 tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg aaaaaaatta taagtcatta
    14941 aaaatgtatg atgcaaaata caaagagttt atcgatgaat tttttccacc atttgactat
    15001 gcaaaagtca atcgcttatt acaaatacga tcagatgtat ttaaaattct tgcagaatat
    15061 gcgatggatg cagatgtgaa tcaatatttt tctgatcgct tagaggaaat tcttcaaaga
    15121 acatattctt ctaatgctaa tgtttttgtt caacttttaa acgttgatat accaaattat
    15181 ttaccagaaa atgaaattaa acattatttg aactatccat ggtcaggaaa gacattttca
    15241 agaagacttt ggggaaatat ttcatcgctt gaacagaaac tctctaatgc tattgtaaaa
    15301 agtgttgcta gtggagaagg cgttatacac gcattaaaca ccatgagatt agactcagaa
    15361 atttgtgaca tgtttaagtt agaagaatca aagtataaca aagcgataga aaatctcgtt
    15421 cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa aatcatattt agaaacaggt
    15481 attgaggaat acaacgtatt gactgcaaaa gatgagagag tttgccggat ttgtggagga
    15541 aaggcaagta agaatcccta taaactgaaa gatgctgtca taggtgaaaa tcgagcacct
    15601 ttccatagtc gttgtagatg tacggatgtt cctaatttac caaaattagg aaaggatatt
    15661 gatgaagaat atgaccgttt atttggcgat ttattagatg agtttgcaca tgattctttt
    15721 ggaattaatt tgaaacggag gaagtagaat gaaagatttt tttgaagcag tactaacaat
    15781 taatgtaaat gctgatattg cagaagccta caaaacagct attgagtctg agaaccatcc
    15841 taatggcttg agagaccatt ggaatggcaa ttatgcctac gtggttattg gcgatcaaac
    15901 tgttaattat caagataata ctccagttga taagaatacc gttaatttaa cgattcaatt
    15961 attatctcat tcattaccaa atttaaaaga aacagttgat tggtatgaaa agatgggatg
    16021 tattgttgtt agaactgact acaaagaagg aaagtctagt aattaggctt ttttattttg
    16081 tccgaaatga cgttaaacta gcgcaatact gggcttggtt gaatggtggg gcgcaactat
    16141 taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg ggcgaaagga gaatgagcat
    16201 gaaacacaaa tcattaatgc caatgaattt gcaatacttt gccgaaggtg acgatcaaaa
    16261 gttttctttt gatgacttca aatcttttgt ggaatccaat gaagaagctc aaaaatttat
    16321 tcagtcacaa tctcaaagtg ttgccgacaa acaattggaa gcttggaaac aaaataactt
    16381 agataaaatt aaacaggata ccatcaagga atatgaggaa tctaagaaaa acaagtcacc
    16441 tgagcaaatt caactggaaa aactacaagc tgaatttgaa gcagaaaaag cgttgcgtgt
    16501 gacaagtgat aataaagcat ttgttgcaga acaaattgct ggattagaac tagatggaga
    16561 gttaaaagag tctatttctc aatttatgct aaataatctt gttagttcgg atacagattt
    16621 cactaagaat gctgttgaag gttttacaag tgttttgaat gcaattaatg agaaacatgc
    16681 agatgcatta aaagaactac aaatgaagtc tgcatttggt ggaactcaac aatcgaataa
    16741 ccaggttcag cagaacaatg aaacattaac aaatccagaa gaacaattag gacaaattct
    16801 tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa catctttaaa taatttagag
    16861 tatttggata tttcaccagc gattaatgct atgcaagtac caaatacacc ttttttaagc
    16921 tatttacttg gtgctggaaa aacagagcca gcaaactcga cagaaattaa atggcgtgaa
    16981 tatgatatca acaacgatga ttcttctgaa aaacttgagg gcggagaata tccagatgct
    17041 gaatcaggtc gaacttggtt taacaactat actgaaattt ttagaaaatc aacctctgta
    17101 tctggtacat tagatgctat taatgtgaat ggagtcggaa atgaattaac taatcaagta
    17161 gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa aattgattac tggtgtaaaa
    17221 gctgatgaaa atggttctaa aggtcgtcga atgaatggga ttttgaactt gatcaattca
    17281 gcaaataagg cagaaacagc cactgcgggt gcagtaacaa gaaaagatat cgatacttta
    17341 tttaaattga tgtatgaaaa aggttatatg ggagaaaaac tatgcttgat ttctccagat
    17401 atgcaggagt taatgactga tgagttagat ggaaaatcaa caaaaattgt tcagttcggg
    17461 gaaagagtaa cttttggatt gcaaattgga aatatcgtgt ctaattacgg tacaggtatt
    17521 gctctactag aaccatcatt gccaaaagga acaattgccg caatcgatac taattatgtg
    17581 aaactacgtc cattacgtga atggagagca gaagaacttg caaaaacaac ggattctaga
    17641 cgtatcggtc ttgtaggtga atactctctt gaatacaatg cttcaaactc tggggcaatt
    17701 ttaaatttaa agtctgaata aaagggagtt agtactccct tttttgatag gaggaattac
    17761 agtggtaaaa aaagatgaaa ctaaaaaaga tgaagtcgtg aaatatagag taggtaaaac
    17821 taaaaatttt gttgggtttg ttcatcctaa aactcgtaga tttatcacag cagattcaaa
    17881 taacgaattt atcatttcta tagatgataa agaagcaatt gcaattttgg aagatgcaat
    17941 tgatgttaat gaaatttagg aagtgatctg atggatgaat cgctaaaaac ggaaatcatt
    18001 gagtctacaa aagaagattt tccagatttg agtgaagaac gcataactaa tttattagaa
    18061 ataattttgc tagaaattga atcatacaac acttgtaaaa atgatatttc atgggaaaag
    18121 ttaaaaagcg tgattagtga agtgttgtat aaaataataa aaaatgaatc agaaaaaaca
    18181 gtatcttcag ttagacgtgg cgatacaacg attagttatg cttcaacaac aaatgatgtt
    18241 agtgagttac ttctaggcta tggtgatttg atacgaagag ttattggttg tggaggattg
    18301 gagttttttt aatgaatgaa gcagatattt tagaaactac ttatgaagat agttgtatta
    18361 ttgaaagact gacggacatt gaagattcta atacaaatat tactattcaa gattacaaaa
    18421 aagtatatga taatcctatt tcttgcgctc tttcacaagg tcaaatcgat ggactagcag
    18481 tcatagaaga tggagagatg gtaaatgttt caactgacac atataaatta tttgttcatc
    18541 ctaagattaa actaaaaaaa ggagatcgaa taacaataac tcaaaaggcc agtggcttaa
    18601 ttttttctct atttgctact aagccttttt actatcctag tcattgtgaa gtgaatttga
    18661 taggaagtga gaaaaatggg taatctcaaa tatgaatcaa atgcagaaaa gatcattgag
    18721 aattttaaaa atatgactgt aattgctcaa aaagaaggaa tatccttcgt taacgattca
    18781 atgaataaag tcgttagttt agctaaacca ttaactcctg taaaatcagg taatttaagg
    18841 cgaggatatc gtgtagtaaa agctagaaaa ctatcaagcg gtcgaattgt tggagaagtt
    18901 ataaacaatg aacattattt taaatatgta gaagaaggtc ataggactaa aaatggtggc
    18961 tttgtcaaag ggaaattcat gttaactcgt gcaacgaatc ttgcaaatat gtcttatatt
    19021 cctcgaagat ttaaacaaat ggcaataaaa atcgttaaga aaggaaagta acatgaaaga
    19081 tgaaatcatt gctgcaatca gtagcaaatt aaaagaaatc tatcctgatg ggacaatata
    19141 tcttgattca gttatgcagt caactaaaga tttttatttc gttttatcag taatggaatc
    19201 tggaactgaa aatgtaggaa ttgatattca aaatgtttct ttcttaattg atattgcatt
    19261 gattgataat aaccctaata gaaatttaat taatgaatta gtctcacgtt gtgggacttt
    19321 ttttaatacg attacaatcg atgagcaaat attatttcca aaagcctatt tacctgatga
    19381 agcagatggt gttcaacata ttcgttttac attagaattt ccacaatata ttgaatggag
    19441 tgaaagataa atgggagaaa aaagaagtaa aaccggaatt atttctgtcg aaaaacctac
    19501 ttggtttcct ttggaagatg agacagggaa ttttccggta tatggtactg cgttgccaat
    19561 gggaacagca gtaagtatca aaccaacagc taattatgaa acaacacaag attatgggga
    19621 ttcagtggtt caagatcaat ttacagcttt tggtggtgca gaagttgaac tggaagcaaa
    19681 tggatatact cataaagttt tatctacaat tactggaggg aaaattgtta aaggtggtgc
    19741 cttgcgttct ggagaagata ttgcgcaaga tggtgcgttt gcatatagac gtaaaaaatc
    19801 gaatgggaaa taccgttata cggtctttta taaaggacag tttgctctag attcagatga
    19861 aacatcaacg attgaaggaa gtaaagtaag ttttactcat ccaacttgga agggttcatt
    19921 tgtagatgta ccaggacttg gatatatgta ttcagttgat gaagatgatg aaggcgtgga
    19981 taaagcaatg atcggaaatt ggtttacaaa agtagcaatt ccaattgaag aagcagaact
    20041 ttcaggaggt acagaataat gtcaaaatat caaacaacga ttaaattaac gaaaaaagac
    20101 gaagaaggta aatatgagca agtacaattt aaatctgctg aatttttacc aggaacagtt
    20161 gtagaagatg ctgcaggagt tatggaagaa atgcaaacag cgactgataa gcaatcagtt
    20221 aaaaaagctt taagtcgtgc ttattcattt attgcagata ctttatttga gggacaattt
    20281 actggtgaag attattgcaa aggaattgat gctcgtgaga ttgcctcatt gacaggaaaa
    20341 ttattgaagt ctgttactgc aggttttgat gaaacttata cagagacgaa aaaaaagtaa
    20401 gtgaggcgct caagtcacct tcatttaagt attcgattac ttaccgagaa ttagatataa
    20461 agacgcaatt actcgaagca ggttggacgt taccagaaat tgaacgtact gacttggatg
    20521 agttaatgcg tctttttgct tttagagatg cggtaaaaga gcatgaagat gttgagtact
    20581 atgataattt cactcaattt taggaggtga tactttgaat aacgatgacc taattctgaa
    20641 gatgatacta gatgaatctg gttttactgc cggtatgaat aatgctgtca aaaagttgaa
    20701 ctcttttgat gaaacaatcg aaagaaaaag tagaaatagt ggcagttcat taggtagtat
    20761 ttggaaaata tttgctggaa gttttctagc tagcggagta actagaatcg taggagctgg
    20821 ctttgatcta atcaagggtt ccataagtgg agcggttgat cgagtagata cgatgaataa
    20881 cgctctacga aatttccaaa atatgggatt tagtaattca gaaattatga agaatatcgg
    20941 aaagaatggg cttttatctc aaggtattca aggacttcct actgctttga atgatgcgat
    21001 aagtcatgtt caacttcttg cttcttctac aggtgattta actcgttcaa ctcaaatatt
    21061 taaagcttta aatgatggaa ttcttggttt cggtggttca actgatcaag ttaatgaagc
    21121 tgttattcaa ttatctcaaa gtttctcaaa cggaaaagta gacgcacaaa cttggaactc
    21181 aatgatcaac gctcaacttg gacctactct ttctgctatt gctaaaaaga tgggaattac
    21241 aatgggagat ctgaaagaag gtttatctca aggtaagatt tctgttgaag aattccaaaa
    21301 tcaattaata gaaatggata ccaaaggtgg cggaggactt aaatcattaa gtcaaatcgc
    21361 taaagattct actaagggaa ttaaaacctc tatacaaaat gcaaaaacag ctgttacacg
    21421 tggtgttggt gaggtaatag aaggattaaa taaagcatta gtagattcag atttaggtgg
    21481 atttaaggga attattgata aagtgggcag ttcaatggaa tcgttcttaa aagtaattgc
    21541 tgcaaatatt cctacagcag tatcattttt aagtaattta tttaacgaaa ttcaaaaatt
    21601 tggttctgct ttgaaattca tgatgccctt tcttgttcct gcagctaccg cttttggagc
    21661 gttcatgttt caacttaaag gtataccagc aataataaaa agcttcaata attttaagaa
    21721 tgccataatt ggcgttggaa attcattaaa gattatgggg gcaatagctg ctgctaatcc
    21781 atttgtttta attattggtg cagtcgttgg tgcaatagct gttttcggtt actttatggc
    21841 aaccaatgaa gagcttagaa acaaggttat atctatttgg aatgatgtga aagattccgt
    21901 aattggtgta ttaaaaaata taaaggactg gggaattgat acttggaatt ctgctaaaga
    21961 aatggcatca aatgcagttg agggtgtcaa agatgcttgg tcaggaacaa aggaatggtt
    22021 ttctaacacc tggaaaggta ttaaggatgg ggccacaggt ttatttgata aaacagtaga
    22081 gacttcaaga aatgcagtcg atagtgtaaa aaatgcatgg tctaatatga agaaatggtt
    22141 ttctgatact tggcaaagta taaaagattc agccagagaa aaatggaatg gaatcaaagg
    22201 ttctattatg tcagttgctg gtccattgat tacaggtata aagaatgcat ttttacatgt
    22261 tactttttat ctagagacct tatggaataa tttagttgag attggtaaaa acgtctttga
    22321 gattttaaaa aatgttatac ttgcgccggt tttgtttatt acctccatga tttctggtgg
    22381 atgggaagaa acaaaaaata atatgattgc tgtt
//
LOCUS       DAZKMK010000044        22160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000044 DAZKMK010000000
VERSION     DAZKMK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.044"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACG8V1_001581"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACG8V1_001581"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0329677.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACG8V1_001582"
     CDS             2370..3848
                     /locus_tag="ACG8V1_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0329678.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACG8V1_001583"
     CDS             3814..5145
                     /locus_tag="ACG8V1_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0329679.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACG8V1_001584"
     CDS             5259..7811
                     /locus_tag="ACG8V1_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0329680.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACG8V1_001585"
     CDS             complement(7818..9257)
                     /locus_tag="ACG8V1_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329681.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACG8V1_001586"
     CDS             9232..9414
                     /locus_tag="ACG8V1_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329682.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACG8V1_001587"
     CDS             9603..9965
                     /locus_tag="ACG8V1_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0329683.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="ACG8V1_001588"
     CDS             complement(10152..11009)
                     /locus_tag="ACG8V1_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0329684.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="ACG8V1_001589"
     CDS             11129..12040
                     /locus_tag="ACG8V1_001589"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0329685.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13693
                     /gene="mdcA"
                     /locus_tag="ACG8V1_001590"
     CDS             12047..13693
                     /gene="mdcA"
                     /locus_tag="ACG8V1_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0329686.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13795..14094
                     /locus_tag="ACG8V1_001591"
     CDS             13795..14094
                     /locus_tag="ACG8V1_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0329687.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14084..15760
                     /gene="mdcD"
                     /locus_tag="ACG8V1_001592"
     CDS             14084..15760
                     /gene="mdcD"
                     /locus_tag="ACG8V1_001592"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0329688.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15764..16357
                     /locus_tag="ACG8V1_001593"
     CDS             15764..16357
                     /locus_tag="ACG8V1_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0329689.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16381..17457
                     /locus_tag="ACG8V1_001594"
     CDS             16381..17457
                     /locus_tag="ACG8V1_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0329690.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17482..18351
                     /gene="mdcB"
                     /locus_tag="ACG8V1_001595"
     CDS             17482..18351
                     /gene="mdcB"
                     /locus_tag="ACG8V1_001595"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0329691.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18462..19226
                     /locus_tag="ACG8V1_001596"
     CDS             18462..19226
                     /locus_tag="ACG8V1_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0329692.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19715..21808
                     /locus_tag="ACG8V1_001597"
     CDS             19715..21808
                     /locus_tag="ACG8V1_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0329693.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21833..22135
                     /locus_tag="ACG8V1_001598"
     CDS             21833..22135
                     /locus_tag="ACG8V1_001598"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0329694.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3786 c         4721 g         6476 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaccagt
    13381 gatgatttat agcgaagata ctacacatat cgtgacagaa gaaggaattg cttatttgta
    13441 caaagcaaaa acaaaagaag aacgacaagc agcaattgca gcaattggcg gtgttacacc
    13501 tcttggaatg actagcacga aagaatcact agatagcttg cgtgcagccg gcatcgtgaa
    13561 attaccagaa gatctaggca tcaaacgtag tgaagcgaaa cgttcattat tagctgcaca
    13621 aaccatcgat gatttgatcg aatggtcaga tggcctgtat gaaccaccaa tgaaattcag
    13681 aacttggtca taaaattggc gagaaaaacc ttttgtcgcc atttacccag atggaagaag
    13741 aaagacgaat aagcagaacg tgtatgctct gttttacaaa aaggggagag atccatggaa
    13801 caattagctt ataaatatcc aacgaaacag ccgatcaata agaaaatcca tgtaggagtg
    13861 gtcggttcag gagatttgga aatcttgatg tttccaacag aaaaaacaga atctactgta
    13921 aaaatttgta caggaagcga tggcttcggc gaagtatgga acaatgtatt aacgagattt
    13981 tttgatcgtt atccaatttc agcagatatc gtgatcaatg acttcggtgc tacaccagga
    14041 gttgtgtacc tacgattgac gcaagcaatg gaggaacttt cagatgaaaa atagttttgt
    14101 agaattaaac gccagagaac gagtgaacgc tctacttgac aatggacgtg aattgattga
    14161 tccattcgat caaatcatcg cgccaagttt accagcacaa ggaatcgtcc ctgaaagtga
    14221 tgacgggatc gttgtcagcc gtggcacgat caatggcaaa caagcagtag taatcgctat
    14281 ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc ggtgcaaaaa tcgttgctgc
    14341 acttgaacat gtgttagaag aaaataaaaa aggaaatgag atctttccag tcattgtact
    14401 agatacaggt ggtgttcgtt tgcaagaagc aaactacgga ctgttatcta tttcagaaat
    14461 cggaaatgta atcattgctc taaaacaata tgtaccagtc atcggtttga ttccaggacg
    14521 tgtaggatca tttggcggta tgtcgattac ttcagcatta atgagttatt taatcgcaac
    14581 aaaaaaagcg agagtcggtt tgaatggacc agaagttatt gaacaagaag caggcgttcg
    14641 agaatttgat tctagtgaca aagacttgat ttggaataca ttaggatctc gtcaacgggt
    14701 agaatcaaaa atcattgatg aattagtgac tgatagtgta gaagcaatca aagctgctgt
    14761 catcgcagca atggacgaac gaaaagacac gcatcgcagt gaaagctcag cattttattt
    14821 atcattactt gattctttag atctttcaaa accaatggcc attgacgatt acaatgaagc
    14881 aattgctaac catcaagcaa aatcaattga cttaccagaa gtagcagaag gaaatgaacc
    14941 agctgcagca agtgttggtt acgattggtt tgcggcacta actggcatga aaaatccaac
    15001 ttcttctatt agtactgtgt attatggtaa atcaagtaaa ttcatagaag aagccatcgt
    15061 gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt gtacgaaacg gtgaagttgg
    15121 tttagttgaa ggtttccgca tgggacaaat tttggatcac gtttacgaag aagataaaga
    15181 caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta ccaagccaag catacggcta
    15241 taacgaagaa ttgatcggta tccatgtggc tttggctaat agtgctgctg cttatgcaaa
    15301 actccgtcaa gctggacatc cagttattgg attgattgca ggaaatgcta tttcaggtgc
    15361 atttttagca catggattac aatcaagccg cttgattgct ttaaacaata aagaaattac
    15421 cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca aaacgttcgg ttgctgaaat
    15481 cgaagcagct gctgaaaaag ttccagctat cgcttacgat atcaataatt atacaaaact
    15541 aggagcgatc tatcatttcc tagaagatat caccgaccaa gctgcttcag aacaaaatgt
    15601 cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat gtccgcaata caaatgacac
    15661 gatgctgaca aatcgataca caaacgaaac agccaaaaca ttcggacgtg tcgaaacaaa
    15721 taaagtccgt gccaaaatgg acgaagaatg ggatgcataa aaaatgattc aagcacatga
    15781 tattgtcctt tttgataaac atgaaataga agggttcacc ttgcctgaat gggtcgttct
    15841 aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg gatcacgcag aaatactacc
    15901 gattggttta cgtggacctg cccgcaatca gagatggtca acagaaatct caaaacatgc
    15961 cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa gaatcgtata gacaagagaa
    16021 aatcatggat tatcctgttt atcagcaatt tcatgctgct agaaacatat tatcaggatg
    16081 taaatgggga atcggcggca gcttaggatt tgaattatcg actgggattc cggcagtaaa
    16141 agaaaccagt gattttgatt tgctgctgta cgcaaattca ccgattgaat tgccaattca
    16201 ggtaattcaa tcgcatcctg ctttttttga gcaattcgat acccaagtag tgactcggca
    16261 aggcggtttt tcactaaaag agtatttgag agagccagag aaaaaaattc tattgaaaac
    16321 agtcgaagga cctaaattaa ccaaagaact ttggtaataa agaaaaagag agagaaagag
    16381 atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact tccagaaagt atcagctgta
    16441 acggctgtta tgatgcaaac gatattgagc tttgctttgg caaacagcca cagcgcaaca
    16501 aatacagctt ttcttggtac cttttacgtc atcgttaaat acacagcacc aatgtttatt
    16561 tttgcaattg tttataatat ggtaaaaaca agtcagcatt tgtcttattg ggaatttttg
    16621 aaagaaaagt tttttgaact agtcgttccg tatcttctat ggacgacggc ttatctttgg
    16681 ctatttccag cagtccaaca acaaactccg tatacggata ctgcaagctt tttactgaaa
    16741 tatgtcacag gtgatggagc gcctcattta tggtataccg ttatgatgct gcagatccaa
    16801 ttgttcatgc cgttttttgt ttggctcggt tacaaagtat ttgctaataa aaaatatgta
    16861 tggccagtat tgattggttc aacgatttta tatgtcgcat ggtatctgtt ctatcaaaca
    16921 caagtattga ctgggcctta tcatgacagc tggtacttat tagatcggtt tgttgctagt
    16981 ttcatgattt atggggtagc cgcatttgtt tatcacgaaa aagtctatca atatttggac
    17041 agagtacgtt atcttttctt acctgttgcc ttagttatcg cgttcttttc tgtccgttca
    17101 ttgctcgcac atccaggaga cttgagcttt gcgaatgcac cttacttgaa tacgatccaa
    17161 agtttataca gtttagtcat catctttgct gtcttcatag gcgcttccaa aatgattgta
    17221 aacgattcgc caaaattgcc attgttcaaa tggttgtctg tttatgctta tcgtacgtat
    17281 ttagccaatg tatttgtttt ccaagtactg ttattattgt ttaaagatag ttggctgcag
    17341 ttaccttcag gagtcatgat tcttgtagct tatctaatga cggcaagttg tgcatttgct
    17401 ttaagctggt tacttcatat catttgggta gctatcaaaa aaggtttcag caagtaatag
    17461 taggaagaag gagtgagtgt catgataaat gacgaacgaa aaaaagcagt gactcagcaa
    17521 ctcgctgcgt tggtagagga agcattgatc gcagaagtaa cattaagtcc aaaacctgga
    17581 ttagtggatg cactggatgc gggtgcacat tccgatatgg actatgcttt atttcttaag
    17641 tctgctaaga cgctaactcc tttttttgaa gaaatggctc aaatagcatg gcatcatgct
    17701 attgatcagg agttaagaga aagaattgct gagattgggc gaaaagcgga aaaggcaatg
    17761 ctgattgcta caaatggcat caatacccat aaaggagcga tttgggcaat ggggctattg
    17821 atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt tttctttatc gaaaatactt
    17881 gatgatgtca gcaagcttgc tgcttttcct gatacaaaat atcgtcataa aaaaaccact
    17941 catggtcata gggtgaagca aaaatatggc gtaaatggtg catatgaaga agcgatatta
    18001 gggtatccgc atatcaggct tgcaattcaa gcagctaagg agctagataa tgattcatat
    18061 gtacagaaac aaatgcatat gttgttagtt ttgatgagtt cgctagatga cacatgcgta
    18121 ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa aacttgctca tgctgctagt
    18181 caaagtgctt taccaaatcg tccatttaca gcactgttgc acttttgtca ggaaaaccaa
    18241 atctctcctg gaggaagcgc agatctcttg tctgcaagct tgttcatgct gaagatagag
    18301 gaattatgga ctgccgacac atacatttgt ccgatgttac tcaatgacta aataagacaa
    18361 aattgctaaa aaaacagttt atgcatctgg ttattgactc ttccttttag caaggctatc
    18421 ataaaaatag ttttttgtaa taaaagataa cgaggaagac aatgaaaaaa atcaattatt
    18481 tagcaatcat tttgttagtg ggcctatgtt tgtttgctct gccgacccaa ggattcgctg
    18541 tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag ttcgggcaga ggttcatttg
    18601 gtggtcgttc atatgggggc agttattttg gcagtcgttc ttacggcaga gattcttatg
    18661 ggtattatgg tccaggtaca ggtccttcta gttatgagca aacgggattt cttgcattag
    18721 ctgttttgtc agtaggaggg gtttctgctt tttctatcgt caagaataaa agacaaaaga
    18781 aaaggctgaa agcggagata ttgtcccgca tgtcaggaac ggctaaagaa aagaaagcac
    18841 agtttctgga aatccaaaag atattcatga agatccaatc cgcttgggaa gaagaaactc
    18901 ctgaaaaagc cagaagctgt tatacagaca agctgtttgc cgaacatcag aaagttctgc
    18961 aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa aataactatt caggaagtag
    19021 gaaattatcg acagatccac gaaaatagtt ttgccatccg aattgatttc agatgctgtg
    19081 attattcagt cgatcgaaaa gatgggcgat tgatctcagg aagtaagaca caacgacagt
    19141 atttttctca gatatggtat tttgattttt ctgagaaaga aaaaagatgg caagccgatt
    19201 ttattcagcc gattatgctg gattgacagg aaatatttat gaacggagtt gtgacaaaag
    19261 ccttgtcgca gctccttttt ctgaataaac ggtgtgccag agatagccta tcggcaataa
    19321 ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat cgcttcttat cgctcaaggc
    19381 tgaacatctc tgtcacaacc tctccactaa atggtgttta aaagcttgca tctgcgataa
    19441 taagaacaac caaacgaaaa ataggaagca ctattttagt ttagttgttc ttattacttg
    19501 atgcaggaca gctttttcac aacctctttt ttgtatagat gggcaaaatt tttgtaataa
    19561 taggcgtatg tctttttcgt tgaggagatg aaaaagcata tggcttattt tttgtttgct
    19621 ttgcttactt tctttccact tcaagtggca gaaatatcta ctttttatta tgtaagcgga
    19681 accatatact tgaaatcaga aggagatcat ttctatgaaa acaagaacaa ttgaaatact
    19741 tcacagaatc atgacagccc aatatccagt aaaagcaaaa catctagcaa gtgagttcaa
    19801 tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg aatatctggt tgagcagtaa
    19861 aaagcttccg gagatccgta caattcgcaa caaaggtttt ttgttgaagc tggaaaagga
    19921 aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa gacggctttt tgaatcggcg
    19981 cgaacgtatt tttgatctag ttttagccgt tgcttacagc cgaatcccga tccatctaaa
    20041 caaaaaagaa gaagaatttc aaatctctaa aagtacatta gatgaagata tgagacggtt
    20101 acgagcaaca ctggtcagtt atgggataga gatcgtcagt tatggaaagc aaggactggt
    20161 ttataaaggt tcagaacgat ctatccgtac gatgatctat gattttatca atcagaatct
    20221 gggaatgacc gacttcttta tgaatcaaga tgcacaatta acaccggctc aaaaaatctt
    20281 tctccattat tttcctttgg tggaaatcaa aaaaatacag gagatctatt ccaataagtt
    20341 gttgaaacaa gaagatgata tttataaaaa acaaatcctg ctttttacat tgatctggat
    20401 ccagcgaatc aggaaacatg aatttatctc tgccattaat tggaaaaata cagaaataaa
    20461 agacagtagc ttttctgcat ttattgacca catgatcgag gaatttcaat tggacaatgt
    20521 cccgcaagta gaaagaaatt atcttcgttt cactattgag acgttcaatg ctagagatat
    20581 ctccaattca atcgagtggg tacaagctca attactaacg atccaattga tccaatttgt
    20641 agagaacgag acacacgtac ctttccatct aaaggaagaa gctttattcg aaaatctcta
    20701 taagcacatg gctgcactga ttgtaagaat caagaatcat atccaagtca tgaatccatt
    20761 gaaagaaaac atccgaacaa attatttccc aatctaccgc gcagtcactc attttgttcc
    20821 tacgatcgaa gaagtaattg gtggcaagat actggaggac gagatagctt ttcttgtcat
    20881 ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg aattatgttt ataaatcagt
    20941 cgttgtctgt aaccatggga tggctactgg aaatctgtta gcagagaatc tgaaagaaaa
    21001 gtttcctcag atcgagatta ctgcagtttt aagttcaaaa gaaatcgaat tagtagataa
    21061 attggatgtc aatctggttt tcagcacctt tcaattgaat tatcgaaata agccgctttt
    21121 agttgtcgat ccgattttaa ctgagacgaa tatcttggtt atagaagact ttttgaaaag
    21181 aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa tcgacggagt tattcactca
    21241 aataatagat gtcatgaaag aaagtggtgg ccgagtagat cgaatcatct atggcaagtt
    21301 agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa agggagatcc agcctatgtt
    21361 aaaagatatt ttgacggaca gcaatatcat gatcaaagaa aaagccacta cttgggagga
    21421 atcgattgaa ctagctgcta gaccattggt taaagaaaac attgtcgaag agcgctatgt
    21481 cgaagcgatg attcgtgcag tgaatgatta cggaccgtat atcgtgattg gcaaacacat
    21541 ggcactagcc catgcccgac cagaagatgg ggtcaataaa ttaggagtca gcgtagctac
    21601 gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat ccagtaaaga ttattttttg
    21661 tttggcagca gtagactcct actcgcatct gaacatcatg aaagaactaa ttgaattgat
    21721 caacaacgaa gagaagttgg atcgattgat tgaatgcaca aatatcgaag atttcaaaca
    21781 actgcttttt tcagtagttg agaaataata aatgatacgg gggagaacaa agatgaacaa
    21841 attgaacatt ttattcgtat gcggtgcagg tttaggaagt agttttgcag cacagatgtc
    21901 tgctgaggat gttttgaaca aattaggagt agaagccaaa ctggaccatt gtgatatttc
    21961 ttccgcagtt tccatgaaac cagatgtgat catcacagca gaaaattttc gttcgcagtt
    22021 cgaaaagttc gcagttgatc ctaaaacggc gatgatctat ttgaaaaata ttgtttcaaa
    22081 aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat aagggagtta tctaaataca
    22141 aaaagaaaag agggtaaaaa
//
LOCUS       DAZKMK010000045        22076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000045 DAZKMK010000000
VERSION     DAZKMK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.045"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACG8V1_001599"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACG8V1_001599"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0329695.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACG8V1_001600"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACG8V1_001600"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0329696.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACG8V1_001601"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACG8V1_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0329697.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACG8V1_001602"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACG8V1_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0329698.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACG8V1_001603"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACG8V1_001603"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0329699.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACG8V1_001604"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACG8V1_001604"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0329700.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="ACG8V1_001605"
     CDS             complement(8297..8767)
                     /locus_tag="ACG8V1_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0329701.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="ACG8V1_001606"
     CDS             complement(8813..9238)
                     /locus_tag="ACG8V1_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0329702.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="ACG8V1_001607"
     CDS             complement(9305..9574)
                     /locus_tag="ACG8V1_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0329703.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="ACG8V1_001608"
     CDS             complement(9585..11180)
                     /locus_tag="ACG8V1_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0329704.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACG8V1_001609"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACG8V1_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0329705.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACG8V1_001610"
     CDS             complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACG8V1_001610"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0329706.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="ACG8V1_001611"
     CDS             15646..16620
                     /locus_tag="ACG8V1_001611"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0329707.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="ACG8V1_001612"
     CDS             16790..18190
                     /locus_tag="ACG8V1_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0329708.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="ACG8V1_001613"
     CDS             complement(18268..18990)
                     /locus_tag="ACG8V1_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0329709.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="ACG8V1_001614"
     CDS             complement(19069..19575)
                     /locus_tag="ACG8V1_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0329710.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="ACG8V1_001615"
     CDS             19698..21497
                     /locus_tag="ACG8V1_001615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0329711.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21565..>22076)
                     /locus_tag="ACG8V1_001616"
     CDS             complement(21565..>22076)
                     /locus_tag="ACG8V1_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0329712.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6111 a         4828 c         3993 g         7144 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttcccctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 gaatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta cacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggttga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg
    21541 ctaactcttt ttgattaatc attcttacat gctgacagca aagtcagggg caaaccattg
    21601 aactgaacca acttttacgc tttcgcctgg ttgaggagca tggatatatt gtcctccgcc
    21661 taaagcaatc gctacgtggt aagttccgcc ttgtgacccc cagaataaca agtctccagc
    21721 ttttgcttga gaaactgaaa tttttgttcc agctgattct tgaggaactg tccaaccacc
    21781 gatgtcacga ccagttactt gcatgtaaac atagcgtgtg aaccctgagc agtcaaagcc
    21841 acttggatct ttaccgcccc aaacatatgg agtaccaata tatttgtaag cttctgctac
    21901 gattgctgca ccattcacac ttccagaagg agctggagca ggtgttacag gttttggttg
    21961 ttcaggtgtt ggtgttggcg ttggagccgg agtactactt cctgtgccgt ttcctgtatc
    22021 aacactttga tctgttgatg gtgttgtagg cgcaggtgtt gttgattctt ctgttg
//
LOCUS       DAZKMK010000046        21603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000046 DAZKMK010000000
VERSION     DAZKMK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.046"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1429)
                     /locus_tag="ACG8V1_001617"
     CDS             complement(38..1429)
                     /locus_tag="ACG8V1_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329713.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1686..5075
                     /locus_tag="ACG8V1_001618"
     CDS             1686..5075
                     /locus_tag="ACG8V1_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0329714.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5078..6499
                     /locus_tag="ACG8V1_001619"
     CDS             5078..6499
                     /locus_tag="ACG8V1_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0329715.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6496..8373
                     /locus_tag="ACG8V1_001620"
     CDS             6496..8373
                     /locus_tag="ACG8V1_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0329716.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8465..9307
                     /locus_tag="ACG8V1_001621"
     CDS             8465..9307
                     /locus_tag="ACG8V1_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0329717.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9459..10334)
                     /locus_tag="ACG8V1_001622"
     CDS             complement(9459..10334)
                     /locus_tag="ACG8V1_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0329718.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10541..11571
                     /locus_tag="ACG8V1_001623"
                     /pseudo
     CDS             10541..11571
                     /locus_tag="ACG8V1_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11660..11965
                     /locus_tag="ACG8V1_001624"
     CDS             11660..11965
                     /locus_tag="ACG8V1_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0329719.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12144..14159
                     /locus_tag="ACG8V1_001625"
     CDS             12144..14159
                     /locus_tag="ACG8V1_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0329720.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14161..14445
                     /locus_tag="ACG8V1_001626"
     CDS             14161..14445
                     /locus_tag="ACG8V1_001626"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0329721.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14471..15844
                     /locus_tag="ACG8V1_001627"
     CDS             14471..15844
                     /locus_tag="ACG8V1_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0329722.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15927..17942)
                     /locus_tag="ACG8V1_001628"
     CDS             complement(15927..17942)
                     /locus_tag="ACG8V1_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0329723.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17964..19178)
                     /locus_tag="ACG8V1_001629"
     CDS             complement(17964..19178)
                     /locus_tag="ACG8V1_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0329724.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19398..20342
                     /locus_tag="ACG8V1_001630"
     CDS             19398..20342
                     /locus_tag="ACG8V1_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0329725.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20638..21582
                     /locus_tag="ACG8V1_001631"
     CDS             20638..21582
                     /locus_tag="ACG8V1_001631"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0329726.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7333 a         3961 c         4085 g         6224 t
ORIGIN      
        1 tatcagggtg gtttatacat agctgttttt tgattattca taactcgaat aagcggatga
       61 gttcttcttc atttgcgaat gacacactat catcaatcaa tatatgagga acatgggcat
      121 tcaaaatggg ttgattcgta acgatcagat catactcgag aagattgtat ttcgacagct
      181 gatcttcagt aatagcagtt gtctcagagt gaattttatg cacagcaaga atatcactga
      241 tatattcttc aatatattga ctatgtcgaa gacttaagtc gctgataatc agtatttttt
      301 tatcagaagt ataatggtga atttcaggat taccgataag catccaaaag atatagctag
      361 ataaataata aatcagattc tgattcagta gagtggaata aaattgaagt tctgcacgaa
      421 atccagcata tagacgtatc tggcggtgtt gcatctgctg tgcaaagtaa gttgcacgga
      481 tcgtcaaatg agtcgtatcg tatgggaaaa tacgggagaa aaaataaatt tgcgttaata
      541 attgaacaac atattcttcg tttttttctt tgcaatcagc ggatgaaaac gaacagtagg
      601 acatattttt tcgtatctgc tcactgattt catttttttc tttctccgaa aattctgaaa
      661 aaatcgtatt gacaaaatag ttcactactt ttatatagct gctgtacttc aattttccta
      721 agagattttt caagattaag aagtcttttt tatcgatatt tatcaactgt acataagagt
      781 ctccagcttt ccattcaaat ggagttcctt gggattctct catgagatgc acaacaaaat
      841 acataataaa gaaagattgc tcatatgggc tttcttcctg aaacaaattc atctgacaat
      901 tattcttgac cataatttct ttgatcttct ttttttcgat tggaaatggc aacgtgcgtc
      961 ttccgtagta acttaaaaag aaaaaggtaa caaagatacg caactggagc tcacttttaa
     1021 ggctagttaa ctgaaaacct tggtcaacga tcacttttaa taaatagctt tctaaaattt
     1081 gattgcattg tttcaactta cgtgtaaaag aagaccggct gatggaaatt ttttcacaat
     1141 aatactcaat cgttttaccc ggattgacaa taatctcatt taataaacga ctgtttgtag
     1201 aactttgcga catttccatc agaacattta aataagctgg tacattcaaa tgttcaagac
     1261 gataccccaa tttttgtgaa gattgaatcg ttacatccgt tccccagcgg attttgatct
     1321 cttcgatata ccttacaaat gtagattctg aactaccaat tcgtttggca agttcttttt
     1381 tagtcatcca cttgtcagaa atggacagaa ttcgaatgat tttttccatc tttctatagt
     1441 ttaattccaa caacctgatc atatgttttt ctccctcaac ccttttcttt caatgaaacg
     1501 ctcattaatc gaaggaatca acttcataaa tgaaaccaat tggtatgaaa attggtgtga
     1561 ttccctttag cgcccttcgt ataataatta tcatattaaa ataaataaaa ttatgtaacg
     1621 cctgtttatt ttaataattt tcactattca cgtcaaaaca gaaaaaaaga aaaaggaggg
     1681 gaaaaatgac gaccacaggg aagaaactga aagttatttt catgctgata atattgagtt
     1741 tatcaaactt tgtgccatta tctgcaatag cagacactac agatgatcca acagttttag
     1801 aaacaatttc agctgaagtc atttcggatc agtctggaaa aaaagcactg aacatcaagc
     1861 taaatgcgaa taacaccagt gctgaaaaga tagaaaaaga aattggtcta gtcgaaaatt
     1921 acttaagtga tgtggaaaga aaagaaggag atggctatgc ttatcaggta aatagcggga
     1981 aaattacgtt ggaaatctca tcaaacacta aacaaactat cgatctgagt tttccaatcg
     2041 atccagcact ttaccacagc caggcaaaca agctgatcgt cgataataaa gaatatgaca
     2101 ttattgatga gacagaaaat aagaaagata cagatgtgtc agtaccaaag ccagacgaaa
     2161 tagaagaaga atcatcaaaa gaaaacgaaa attctgtcag cccatttaca ttgcctacat
     2221 tatccttgcc agctgtgagt gtgccatcta atcaaacgat tcctacagaa tatacaacag
     2281 atgatcaggg cacttatcct aaagccagtt ggcaacctac aggaaataca aatgttcttg
     2341 atcatcaagg caataaaaac ggaacaaatc aatgggatgg tataaattct tggaatggag
     2401 atcctaatga tcggacccat tcgtatatcg aatatggagg aaccggtaat caagcagact
     2461 atgcgatacg aaagtatgca aaggaaacaa gtacacccgg attgtttgat gtttatttga
     2521 atgctcgtgg aaatgtacaa aaagatatca cgcctcttga tctcgtattg gtcgtagact
     2581 ggtcaggaag tatgaacgac aataatcgga tcggtgaagt aaagattggt gtcgatcgtt
     2641 ttgtcgatac tttagcagat agcggtatca cagacaaaat caatatggga tatgtcggct
     2701 actcaagcga aggatatagc tacagtaacg gtgcagtaca gatgggttca tttgattcag
     2761 tgaaaaatca agtaaaatcc attacacctt cacggacaaa tggtggtact tttacacaaa
     2821 aagcactaag agatgcagga agcatgctat ccgttccaaa tggacataaa aaagtgatcg
     2881 ttttgctgac ggatggtgta ccaacatttt cctataaagt acagcgggta cacgcacaat
     2941 caagcagcaa ttattacgga actcagtttt ctaatacgca agatcggccg ggaaatactt
     3001 ctctaatctc aagaatctat gatgcacctg accaaaacaa tctatccaga agaatcgaca
     3061 gtacgtttat cgcaaccatc ggagaagcga tggcactcaa agaacgagga atcgaaatac
     3121 atggtcttgg catccaactt caaagcgatc cggcagctgg tctctcaaaa gcagaagtag
     3181 agtctcgtat gcgacaaatg gtttcatcag atgaaaaagg cgatctttac tatgaatcag
     3241 ctgatcatgc aacagatatc tctgaatacc tagccaaaaa agctgtacag atctcagcaa
     3301 ctgtaagcaa tggacaaata aatgatccaa tcgcagaacc attcatttat cagcctggta
     3361 cactttcagt caagagtgtg gggacaagtc ctacaacggt cactccatct atttccatag
     3421 aaggaaatac catcaagagc aatcagatct atttaggaaa agaccaagaa atccaaatcc
     3481 attaccaagt gagaatccaa acagaaaatg aggacttcca tccaaatttc tggtatcaaa
     3541 tgaacggcag gacaactttc cagccaaaca ttgataccaa tgaattagct gaattcggta
     3601 taccatctgc taaagctccc ggagtcagtc ttcacatcaa aaagttatgg gaagaatttg
     3661 acaacaatct agctgatcgt ccagatcaag ttacttttga gattcaacgg gaacatacga
     3721 caaatgctgc agcttggaaa aacggatata ttcgaatcat taaaccagct aaagatacaa
     3781 caaatacgtg ggaacgtgca gacattgaca aattatctgc aaatagcgga gaaagttatc
     3841 aagagatatt atcactacct caatacaata atcaaggtca agcattcagt taccaaacaa
     3901 tcaaagaatt acctgtacca ggatacgatt ctcaacaaat agatgcaatg acatggaaaa
     3961 atactaaaca attcacaccg ttaaacttga aaataacgaa aaattcctct acaggtgaaa
     4021 aggatcttat tggcgctgtt ttcaaattaa caggagattc tattgatact ttactaacag
     4081 atcatggcga cggaacctat tctcttccag aaaatgtcaa attgcaaaaa gaaatgacct
     4141 atacgctgac agaaacaaaa gctccagaag ggcatggatt aagcaaaaag actacttggg
     4201 aaatcaagat cgcttctgat ggtacggtaa ccattgatgg aaaaacagtc actacttccg
     4261 atgatacgat ccagttgact attgaaaatc cttttgttga agttcctgta gcagtacgta
     4321 agtatgcgat gcaagggacg gacaaagaga taaatcttaa aggagcagca ttttccctac
     4381 agaaaaaaga agcaaatggt acttatcagc caattgacag ccaaacaacg aatgaaaaag
     4441 gtcttgccag ttttgattca ctcacacctg gtaaatatcg agtcgttgaa acagctggtc
     4501 ctgccggata tgatacttcg ccgggaaatt atgaattcca aatcgataaa tatggaaaaa
     4561 tcatttacac gggaaaaaat accgagatga caaataatgt atggacgctc actcatcaaa
     4621 atcgactaaa agcgtttgat ctaacggtac acaaaaaaga agacaacgga cagacattaa
     4681 aaggagcaaa attcagactg cagggaccag aaatggactt agaatcgcca aaagatggac
     4741 aagaaacaga tacctttcta ttcgaaaatt taaaacctgg aacttatacg ctgaccgaaa
     4801 cttttacacc agaaggatac caaggtctaa aagagccagt tactatagtt atacacgaag
     4861 atgggtcaat tcaagtggat ggacaagatc atgaatctgt tctgtcacca ggagccaaaa
     4921 acaaccagat ttctttagac atcacgaatc aggcaaaagt accattacct gaaacgggag
     4981 gaattggccg tttaggaatc tatctagtag ggatgattgg ttgtgcgttt tctatttggt
     5041 atcttttttt gaaaaaagaa agagggggca gctaaatatg aaaaaacttg gttggcttag
     5101 tatgtgtctc ttcttgttac tatttaaacc agcttttact caggtagcaa cagaaacaga
     5161 aacagaaatg gttcagatta ctttacacaa attgcttttc ccaaacgggc aactgccgaa
     5221 aaatcatcca aatgacggac aagaaaaagc tttattacaa acgtatcgag gattaaatgg
     5281 tgtcacattc caagtttatg atgtcacaga ttctttttac catctacggg aaaagggcaa
     5341 aacggtagaa gaagcacaag cagagatcgc aaaaaacggt gcgtcttccg gtatgtttac
     5401 cgcagaagca acaactacaa ctcttaacaa cgaagatggt atcgcttctt tttctctggc
     5461 cgctaaagat caagaaaaaa gagataaagc gtatcttttc attgaatcca aagtaccaga
     5521 agtcgtcaaa gaaaaggcag agaatatggt agttgttctt cctgtacatg gacaaaacaa
     5581 tcaaaaactt tcaactatcc atttgtatcc taaaaatgaa gaaaacgact accctgatcc
     5641 accttttgag aaggtattag aagagcctag aaatgatttt acgattggtg aaaaaatcac
     5701 ttattccttg catacgacaa ttcctgtaaa tatccttgac tatcaaaagt tcgaattgtc
     5761 agatagtgcg gatgaagcat taacgttttt acctaatagt ttaacgattt catcgaatgg
     5821 agaaaagctg acagaaggct ttgtcataca caagaaacct cacggatttg atgttttatt
     5881 ttcgatccct tcgttggaaa aatatgctgg aaaaaaactg accatttctt atcagatgca
     5941 gctaagcagt acagcacagg cgaacaagga aatcaacaac aacggaacac tggattttgg
     6001 ttttggtgtc agtacaaaga aagtctctgt atatacaggg agtaagcaat ttgtcaaaat
     6061 cgagacaaat aaaccagata aacgattagc tggcgcagta ttccttatta aaaacaaagc
     6121 aggaaattac ctccagcaaa cagccaacgg atacaagtgg acaaagaacg aatcagatgc
     6181 gcttcacctg atttccgata aaaatggcgc tttttcaatt tccgggttga aaacaggaag
     6241 ttatcgatta aaagagatcg aagcaccttc tggttatatt ttaagtgaaa cagaaattcc
     6301 gtttaccatt tcaacttttc tttctgagga taaagaggcg gacagtatat tgaaagtagt
     6361 caataaaaaa gaaaatagcc gtccatttct tccaaaaaca aacgaaacga aaaatacact
     6421 tttaggcgtt gttggtatgg tattcgcaag ctttgcaatc tggttgttta tcaaaaaaag
     6481 aacaggagtg aaaaaatgaa aaatcataaa aaaataaacg ttatgttagg agtccttttc
     6541 cttattttac cattactcac aaacagcttc ggcgcaaaaa aagtgtttgc agaggagaca
     6601 gcagctcaag tcatccttca taaaaagaaa atgactgatt tacccgatcc tttaatccaa
     6661 aacagcggga aagaaatgag cgaattcgat caataccaag gattagccga tatttcattt
     6721 tcagtttata acgtcactca agaattttat gcgcaacgag ataaaggagc gtccgtggat
     6781 gcagcaaaac aagcagtcca gtctttgact cctggtacac cagttgcttc aggaacgaca
     6841 gatgctgatg gaaatgtcac tttatcttta cctaaaaaac aaaatgggaa agatgcagtc
     6901 tacacgatca aagaagaacc aaaagacgga gtgtcagctg ccgcaaacat ggttttagct
     6961 ttccctgtat atgagatgat caaacaagca gatggctctt ataaatacgg gacagaagaa
     7021 ctagatacta tccatctcta ccctaaaaat acagtcggta atgatggaac gttgaaagtt
     7081 acaaaaatcg gtactgccga aaacgaagca ctaaatggag cagaatttat tatttctaaa
     7141 gaagaaggaa caccaagcgt caaaaaatac atccaaagtg tcacagatgg attgtacact
     7201 tggacaactg atcaaaccaa agccaaacat ttcattactg gtcattctta tgacatcggc
     7261 aacaatgact ttgccgaggc atctattgaa aaaggccagt tgatcgttaa tcatttagaa
     7321 gttggaaaat ataatttaga agaagtaaaa gctcctgata atgcggaaat gattgaaaag
     7381 caaacaatca cgccttttga gatcctggca aatagccaaa caccagtaga aaagaccatc
     7441 aaaaatgata cgtctaaagt tgataaaaca acacctcaat tgaatggaaa agatgtcgca
     7501 atcggtgaaa aaattcaata tgagatttct gtcaatatcc cattaggtat cgctgataaa
     7561 gaaggaacgc aaaacaagta cacaacattc aaacttatcg atactcatga cgctgcttta
     7621 acatttgata atgattcttc aggaacgtat gcttatgcct tatatgatgg aaataaagaa
     7681 atcgacccag taaattattc tgtcactgag caaacagacg gattcacggt ttcagttgat
     7741 ccgaattata ttccttcatt aactcctggc ggtacattga aattcgttta ctatatgcat
     7801 ttgaacgaaa aagcagatcc aaccaaagga ttttctaacc aagcaaatgt cgataacggg
     7861 catacaaatg atcaaacacc accgtcagtc gatgtcgtta ctgggggcaa acgatttgtt
     7921 aaagtagatg gtgacgttac atcagaccaa acacttgctg gagcagaatt cgtcgttcgt
     7981 gatcaagata gtgacacagc gaaatattta tcgatcgacc catccacaaa agccgtcagc
     8041 tgggtatcgg cgaaagaatc agcaacggtt tttacaacca caagtaacgg tttaatcgat
     8101 gtgacaggtc taaaatatgg cacgtactat ctggaagaaa cgaaagcgcc agaaaaatat
     8161 gttccattaa caaaccgtgt agcatttact atcgatgaac aatcttatgt aacagcagga
     8221 cagttgattt ctcctgaaaa aataccaaat aaacacaaag gtacacttcc ttcaacaggc
     8281 ggtaagggaa tctatgtgta tatcggtgca ggagtagtcc ttctactgat tgctggactg
     8341 tactttgcta gacgcaagca cagtcagatt tagtcttttt gcaagaattg gaaaaaggag
     8401 cagcggatta cgcaaatgct cctttttctt ttattatcaa acagtcatca caaggagaac
     8461 acaaatgaaa ccaaagaaaa ggaaacttct agatctgctg atgcttttct tgcttctctc
     8521 aggaataggg atactagctt atccatttgt tagcgacgcg ctgaataatt atctggatca
     8581 gcagatcatc tctcattatc agcaacaagc ggtcaaagaa aatgaagaag tcatggctaa
     8641 aatccaagaa aacatgacaa agaaaaacca gcaattagca aaagaaggtg gaaatccagg
     8701 tgcagatccc tttactaaga aaaaagaacc tgcgaaaaag gatagaacct attttgagaa
     8761 acatacgatc ggtattttga cgatccctga gatcaatgtc catttgccaa tttttgatga
     8821 aacgaatact gtgctgctag aaaaaggtgc ctcattattg gaaggaacgt cttatcctac
     8881 ggggggagaa aatacacatg ccgttatttc cagccatcga ggactgcctc aagcaaagtt
     8941 gttcacagac ttaccgaaac tgactttagg tgattctttc tatatcgaaa tcaatggacg
     9001 gacactcgct tatcaagtcg atcagatcaa gacggtagaa ccgactgata caaaagatct
     9061 gagaatcgaa aaagggcagg atttcgtgac actcctaact tgtacacctt atatggtcaa
     9121 cagtcatcgc ttacttgtca gagggcaccg ggttccttat catgcaaaag aagttcaaaa
     9181 agaagtacag aaagtatccg agaacaaacg atttttcctg tatggattgt tagctgcatg
     9241 tatactgatt ttcctccttc ttatatatct ttttcgtcaa tcgttcaaaa taagaaacag
     9301 aagttagcta ttcctcattt ctataatata tctattcgaa tcgttccgta aacaaacagg
     9361 ctgaggtttg aaaaagaaaa cttcagcctg tttttatgat gatatcaaag agagcctatc
     9421 aaacactatc tttcttatta ctaaatgagg atcttttctt aagttttccg atattcttta
     9481 agaaggcctt tttgcatcaa ttcttgtttg tttttcaagt tgcggatatt ctcttcaaaa
     9541 ttttgagaat atatcactga gaataacgta tttaatacgt aatcgaaggc aatttgagag
     9601 gagaaagtcg ctactttagc aaaatcatat tcctcctgcg atataacaag acacatatca
     9661 gcctgtttgg ccatttctga atgctgattt ccggtaatca gcaaggtagg ggcctctaca
     9721 tgcttcaaat aggtcattat tcgttcataa tgatggatgc gaccgctgta gctaataaaa
     9781 atggcgcaat cctcgctagt caaattggct gcattccaag aagaatcact gtattcttca
     9841 gccatgatca aaaacttatt caacttgacc attttattct ggaatttcct tgccgtgatt
     9901 tgagaatcac cttttgcaaa aagaaagatt cgctgtgcct tgttcaatac tttaccgatt
     9961 tgatctaaat cttcatgttc aatctgcaat tgtgcttttt ttattgactg gatcgataaa
    10021 tccgccattt tctttgcaat ttgctgagtg ctatcttcac ttgaaaaagg aaaattagga
    10081 tcaatcgttt gattgaactg agcaatcaat tgatgaactt cccgccccag ctcgaaacgg
    10141 aattctttaa atccgctgaa tcccattttt ttgcataaac gaatgatagc tgaatgggaa
    10201 gtatacgtag cttttgctaa ttcttcaatc gtcatgtaga ctgcagcttc gatattccta
    10261 cggatgtaat cgacaatccg tttctctgtc gtggtaaaca actcttgttt tgttagtttt
    10321 tcttcaataa gcatgatctg acctccaaat tctatcttac tgataaacac ttggttccgc
    10381 tactatcttt ttgattttta tccttacttt gaacattaat ttcaccaaac cgaatgataa
    10441 caagtgtttt tctgaacttt tttaccagaa tagctattat aaatactttc tgcgcttttc
    10501 taggatatac tgaaaacatc aataaaagga ggcaccactc atgcttacaa tcgcttatct
    10561 tggcaatgga aagagtacaa atcgctatca tttgcctttt tcaagaaaac ttacgaacaa
    10621 aatcaatatc aaaaaaattt tttccccatc tgaaccagtg tggaaaacat tcgaagacat
    10681 cgaatatact gatcagctgt ctgatctttg ggatgatcct gagatccaat tagttgtcat
    10741 cactacacct agtcaatttc attatgaata tggaaaaatg gcactcgaac atggtaagaa
    10801 tgtactagta gagaaacctt ttgcagaaac gtcaaaggaa gcagaagagt tatttgcatt
    10861 agcaaaagaa aaaggtctat ttatccaatg ttaccaaaac agacggttcg attctgactt
    10921 cttgacagtc caaaaagtaa tcgaaagcgg aaaattaggg gatattttag aagtagaaat
    10981 gcattatgat tatttcagac cggaagtacc agaaaatacg catgaatttt ctactgcgaa
    11041 cagctattta tatggacatg cttgtcatac agtagatcaa gttctttctt atttcggcga
    11101 accagatgat atcagctatg atgtacgtca acttttaggg aaaggacgaa tgaatgatta
    11161 cttcgatctg gattttttct atggaaatat gaaaatttcg ataaaatcca gttatttccg
    11221 catcaaagaa cgtccaagtt tcgtcgttta cggaaaaaag ggatgttcgt gaaacaaaca
    11281 aaagatcgac aagaagaaca tctgaaaatg ttttacatgc ctgatcatga agactttggt
    11341 atcgatttgc ccgaacatta tggtgtattg acctatatag atgaacaagg gagttatcat
    11401 gaagaaaaag taatttccga ggttggtgat tacagccgtg tctacgaagg gatttacgag
    11461 acattagtta atggcgcaga aaaagtcatc aaagatgaag aaacaatctt acaaatgaaa
    11521 attttggaag aaggaatcaa aagcattcaa gaatcagaag gaaaaatatg acatatttgt
    11581 aagaatcggg tttgtttttt gacgcataaa ctgtcttatt gtacgatgaa aaagtataga
    11641 actactagga ggaaccaaca tgacaaatta tgacggtgaa aaacacaaac gattaagagg
    11701 ttcaatggcc cctaaaacag aagaagaagt ggctttagca ggaactaatt ctggtgaaac
    11761 agaagacaaa gaagaatcat tagctggcac aaactctggt gaaacagaaa ataaagaaac
    11821 agctttatct ggttcaaatt ctgtagaaga ggaaacaaag cctaatgagc ccaatcactt
    11881 tgaaaaagat atcgaaaaag cggttgaaga tgtcaaagaa aaatttgaag aactgacctc
    11941 aaaaaataaa catgatcatc attgatcgga cgaaatcaat tcataaaagc aggagacatt
    12001 tggtcacctg ctttttttct gtatccattc gatagaaatc gttcggtaca gtcgtaaaaa
    12061 aaatccaact gtaagttgta ccaaacaccc ctcgatttaa aagcgctacc atactagaat
    12121 ataagtagga ggtgaacagc gctgtgaact acaaaattag ttatctcctg aaaacaccaa
    12181 gagaaatcag tctagaaaaa gcagcagaga tcatcgaagt aaatacaagc gaggcgatca
    12241 aacaattaac tccttttgcc gaatatgtgg atgatacccg tatccgtata aagaaattcc
    12301 gagcaatcga ctgggcagaa aaatttttgg atattactcg atttgaagct gtgtataccg
    12361 aacaagagcg aagagatctt ctttatttga tggtttattc gactgcaacg tctgtttctg
    12421 tttttcattt tcaagatttt cttcaggttt ctaaagggac agtcctgtca gatatcaaaa
    12481 agataagaaa tgaattagaa gtggaagatt gtcagctgtc gtatactagg aaaactggtt
    12541 ttaggctgct agggtcagaa gcaatattaa gaagagaagc aaaaaatcgc actgctcgtt
    12601 tgatgcagtc acttgctgga agatttgggc tcacgtattt aaccgcaacc ctttccattg
    12661 attattacgc caaagttaga gatttgctag ataaaacgat ttcactatca gatcttcgat
    12721 tcgcaccaaa tcgaatggat gaattagctt attatttgag tttgacaacc attcgaattg
    12781 gaaaaacatc catggaactg gtaaaagatg ctgaactgtt ggcttctctt gcagtcactg
    12841 acattagtcg acaagtattg actggccttg gctattccga acatcaaaat tcggagatta
    12901 tatttttctc tctttgctta gcatcgatcg ttgaaggaga tgtccaggag cctgcacttg
    12961 attttttgct ggaatgttct tcagaagtca tccatcgcat ggaattcctg atggccgtag
    13021 aatttgcttc gtttcgtgaa ttattgatga atgtctttta tcacttggtc cctgcctatt
    13081 ttcgtatttc ctattctttt tatttaccta atgtgatgat cgatcaaatt aagcatcaat
    13141 atgcttccat ctacgaaatg acaagaaaag cattacttcc gttagaaaga cgaataggga
    13201 agagcatccc tgaagaagag attggttttt tcactatact atttggcgga gagatcagaa
    13261 aagcggacgc agaagaaaga aatagaaaaa taagagccgt gatcgtctgt ccaagtggca
    13321 ttagctcttc attgatctta aaaagtgaac ttcagcaatt attcccgatg atctttttta
    13381 cagagactaa ttcagctgac cgcttacacg aggttgacga aacttcttac gatattattt
    13441 tttctactgt acctctcaca cttactgata agaaaaagtc tctctatgtt ctgcatccaa
    13501 tcatgactga cttggaaaaa aatcagctga tcaatcaagt acaaagagat tggctgatac
    13561 caggattttc aatgccttct gcccaagaat tgttgtcggc gctactgcct tatatcgaat
    13621 taaaagaagg cgtagacgaa gaaaagctct atcaagtatt aaatcgtaaa atgaacaaac
    13681 ttttagaaaa gaaggaggat caacgtccta tgctatctga attattaaca gaagaaatga
    13741 tccaattatc ggatcaacca aaaaactggc aagaagcaat cacatttgcc gcacagccgc
    13801 tattatcaga aaataaaata gaagaaagtt atatcaaagc tatgatcgag cgcgtggagc
    13861 agtatggtgc gtttatccat attggcgatc acatcgcatt acctcatgct cgtccagaag
    13921 atggcgtaaa cgaagtaggg atgtctttgc tgaaacttca acaacctgtg gatctgcttg
    13981 ataatccagc ccatcccatt agtttgttca tctgtttagc tgctatcgat aatgaggcac
    14041 atcttcgggc tttggcaaat ttaacaaagc tactttctaa taaacaaaat cttcaagatt
    14101 tattacacgc atcaacaaaa gaagaaatat tagcgatcat aagaaaagga gacgaatgat
    14161 atgaaaattt ttgcagtatg tcaaagtgga ttaggaacaa gttttatggt agagatgaac
    14221 atcaaacaag cacttgcaga tcttggagca gatgtggacg agtttgaagt aagtcatacg
    14281 gatgttggaa gtgtatctag cgatatggct gattatttct tcattgagca ttctttgaaa
    14341 gatacgctaa cttctatacc tgatgaaaag ctcgtacctt tgcaatcgat cattgacagc
    14401 gaagaagtaa aagaaaaagt aacgaagatc ttgtcaaaag aataaaaatg attcaaggga
    14461 ggaactatca atggataaat tgttgaattt gtttgtgtca atcgtcagtc agccctccat
    14521 cctcgtttct ttgattgcac tgcttggtct cgttcttcag aagaagaaat tttcagaagt
    14581 gatccaagga actatcaaaa catttgtcgg attcctagta ctgatcggcg gtgctggttt
    14641 aatcagtaat tcactaaccc cttttgcttc gatgttccaa acagctctgc atactcaagg
    14701 agtagttcct agcaatgaag cagtagtggc gattgctttg caacaatacg gtacacctac
    14761 tgctttgatc atgctagtag ggatgatcgt gaatatttta cttgctagat tcacaagata
    14821 taaattcatt tttcttacag gtcaagcaat gctttatgtt tcttgtctaa cagcagtcat
    14881 tttagtaagc gcggggttta ctgcaaattt gtggatgatc ttattaggcg gggtcttcga
    14941 aggaacacta ttaactatta cgcctgctct ttgtcaaccg tttatgaaga acatcactgg
    15001 aaacaatcaa gttgcgttag ggcataccgg aaatatcggt tatgcttcta gtggatggat
    15061 cggaaagttt tttggaaaca aagaccgttc tacagaggac ataaagattc ctaaaagttt
    15121 cagctttttg cgagattcta ctgtatcgat catgctcttg atgtcgatcg tatatgtcgc
    15181 acttgctttg ttagctggtc ctggatttgt tgaaagagaa ctaagcaatg gagacaacgc
    15241 tgtaatcttt gcactgatcc aagctggaac attcacagct gggtttgtta tcgtccttca
    15301 aggggttcga atggtgttgg cagaaatcgt tccagcattc caagggattg ctaaaaaact
    15361 cgtacccgat tctaaaccag cattagatgt tcctatcgtt tttccatatg cacctaatgc
    15421 tgtattaatt ggcttttttg ttagcttctt tgtaggaaca ctatccatgt ttgctatgat
    15481 aggcttgcaa actacagtta tcattcctgg ggtagtagga catttcttct gcggagctgc
    15541 ttctggtgtc ttcggcaact cgactggcgg tagaagaggg gcggtattag gttctgcttt
    15601 caatagctta ttgattagtt ggctgccatt atttatcctt ccagtattag gagatcttaa
    15661 aatcgcttct tctacatttg ccgatactga ttatctagta ccaggacttg tacttgggaa
    15721 actaggagaa tacggtcaaa tagccttgac agttggaatc atcgctttct ttttacttgt
    15781 cgtattatct agctttcttt ttgccggaaa aagaacaaac attgtttcta aagaacagaa
    15841 ttaagcaaac ttcaaaataa aaattgcttg gacaatatca gaagaagatt taaagtcttc
    15901 tgatatgtcg caagcagttt tttgttttat gcatttattt ttgaatagct tgttaagaca
    15961 agtggctgtt ttgctagaag aggataactt ttaccattca ttttcactgt tccgcctgtt
    16021 ctatcagaag taatcacgat cttatctttt gttttttcaa gcgaaatctt tccatctttg
    16081 ataggtagcg tacaattcag tgtttcaaat gaatctagat taggttgtat cataaattct
    16141 tcataacctg gctttgttgg atacaaaccg ataaaatagc gtcctaaaag atagattggg
    16201 cttgctcccc atgcatggca taagcttttt ccataaggat ctccatacat actgtacttt
    16261 tcatttcctg tttgagtagg gtcgtattct tcccagaaag ttaccgcatc atgagataac
    16321 atccctcccc aataatcaag aatcgtttgg tagacgatat tcgtctgtcc gagtttgcat
    16381 aaagcatctt gttcatagaa tttgaaatat ggagtagtaa tctgtgtgat agtgtcattc
    16441 aataagacat tctttaagat cgattgctgt tggtctttgt tagcaaaatc gaataaaaca
    16501 gcgaaaatat tcgcatgacg cgtcacattt cttctcccag attcatagca atcgataaaa
    16561 gcgccaagtt cttcatccca aaagtagtca aataggtttt gtttcaatag ttgatatttt
    16621 tcttgataga gatgagtatg ataattttcg ttctctccat atgtttcagc taagatatct
    16681 ccgcaagtga tcatggaatg ataggctttc agtaataaga cttgttctgc agcaagcgtt
    16741 ccttctttat ccatttcaga ccaatcaata aatatccaat cttttgctct cccataaata
    16801 aaccctaatt cattcgtttg ctccatcaag tatctcatta atgattgcat cttagggtag
    16861 acgatgcgta aaaattcata gtctttcgtc atcatatagt ggttttcgat accaatcaac
    16921 cacaagatcg aatagtcaac gatagtattc atatgctgct tgatttcatt ttgtcctctt
    16981 aatgctaata tcgttcgttt attgatttcc tcgtcaaaaa aaagatattg attgatcatg
    17041 aatgattgat acgcatctcc agaccaaatc cagcgatctc gtttgatacc gtcgataaaa
    17101 aacaaatcac tacataactt gaaggcttct tctgatactg accagatctg attcagtaaa
    17161 gaagaatcgc ttgaaaaagc agcttgtttc gaaagagata aaaattcatg aaaaacttga
    17221 agttgaacca ttccagtaga aacctctggt ataaacaaat agcgaaaagc gcgtttacga
    17281 gctttcattt ttcttgtcat tcctatttgc ttatagtagc aataatcgat atccatagct
    17341 tctacatcgg actctccata acatagagtg atagattcga tatggtcatc cagagatatt
    17401 agttctacac agccattcac tgctcgccca aaatcgaata agattcctcc gccaatctcc
    17461 gtttcagtga ctggatggac gcattctttt tgataataga tttgatttgg atcttttgcg
    17521 atatcgacat aaagcggact ccagccagct ggatattctt caataaaatt acttgctgtc
    17581 cagccaatat ctgattttac ttcgtctccc tcgataaaca tcgctggtag ccctgatgta
    17641 tgtcctacaa agatacgaat ttttgcttta ccagctggac aagtcagcca ttttccacat
    17701 ggatgttttt gtccattaat ttctacatac ccaactcctt ggatcgtcac tttaaacatg
    17761 gaaggttgat caagaaagta atatctctta aacttgacgt tccgatggca atcatccatg
    17821 taccagtacg ctggccagtc aaaacctcgt tcttcccgtt ggaaattttg tagcagtcca
    17881 tggcggatct caaagtctcc aggataccaa agccatctat ctttatgttt ttcatttgtc
    17941 attttacacg acctccatca aatttatttt gttgtcaatt gttcttttat ttcttcatct
    18001 actcttatca ctgtctttct attcgtcctg tgtactacta gaaggctaat aagcgcgcta
    18061 atcaacagca agatactgcc attgacgaaa acagcaagtg ttttactccc aagagaagca
    18121 aaagagagaa agagcaaacc atatggtgca atgaaagtac ctaaattagc gccaaccaat
    18181 aagattgaag tattgaaact ctccgcgtac ttgggcgctt ggatacttac ttggttgaat
    18241 aagtaaggaa tgaataacga aaaagaaatt cctgttagga tcgcaccaaa cccaatcaca
    18301 tacaggttta tcacagtggc aatcaataca aagccgattc ccatcagtag taaagaaagt
    18361 gtaagcatat agcgatgtgc ttttgtctga agccagccaa aacaaactcc ggcagccata
    18421 cttgccaatt gcatgattgt taagatattc gctgcattag tttcgtttcc tagagaataa
    18481 gtcgtcagga ctgtggctaa tttaatcgat acagcattat ataccacgat gaccagcaag
    18541 agaaaaatac cataactaat cattttagga ttcgttttga ttacttgtct tgttggggct
    18601 gtgccatgcg attgaagttc agttgtttca ggtgtaggaa caaacaaaac aaacaagaca
    18661 agaataggta agctgatggc gtagactaaa aaggtatatt gccaaccgaa ctgtactagc
    18721 tgtcctgcaa caaatgtcat taaagctgca ccaaatcctt gaaatgcact ttgaaaacca
    18781 atcatagagg ctcgttcatc tccgtgataa aaggcagcaa tcatgctgac tgccaaagaa
    18841 ttgatcaatc caaagccaat acctagtcca gctcttgaag ctaagacaat actgtaactt
    18901 tgtgagaaga gggggaggat accacaaatc gatgcaatca ctaatccgat gatcacagtt
    18961 tgtttttgtc cgattttcct agcaataaaa ctactaatca aaacaaaaat agtgaccata
    19021 aaagagggag cagtcgttaa taattcaaca gcagataaag aacgcgtggc ataagtagct
    19081 gacatactgg gaattgcaga cgtgactgca ccagcagaag tcaaaataag agagactgac
    19141 agcagagaaa atttcatcat ctttttattc ataagcatgt tcctttcatt tttgtaagcg
    19201 cttatcaaaa atatagtatg aagtggttat tctctcaata gcggaatcac aggaagttca
    19261 tgtcccaacc atagaaagtc tttatgaaac caaaaatagc tttgcatata atagcagtac
    19321 agcgtgaact aataagcgaa agaaaccgct tacaaaaagg agggagtcaa ttggaactag
    19381 ctgaattaga catttttttg cgtaacgaag aatcaatcga aactctgcag cgtgaacaag
    19441 gaatgaatat caatgattta aacttagctt accatcttca gacaccgcca aaaatccacc
    19501 gaggggatct atttcaacag ggaaatattc atatcagtaa acaccgtcga tatgcggaag
    19561 ttcccgagca tacacatgat tttgttgaaa tcaattatat gtatagcggt acatgtttac
    19621 aaattattaa tggagaaagg atagagatgg aagaaagtga cgtggtgatc attgataaag
    19681 aagctactca aagaatcgaa tacacagggc aagaggatat tttaatcaac atcttattaa
    19741 aagaagaaac cttatctaca gagatattga atcatttggc aagcacaaat aatctacttt
    19801 cttctttttt gataaatgca tcaaaagaaa aaggggaaca tacacaattc attcattttc
    19861 gcggcggaaa aacaagtcgt ttgacgcatt tgattgaaaa tatgctgttg atttattttg
    19921 atacatacag tcataagatc cgttcgttaa atctctatac atcactatta ctgatcgaat
    19981 tgactcattt attagaacaa caaagctttt catcagcaga tggatgcgca gaaaatgaga
    20041 tgcttagagt attagattat atcgataagc attttacttc ttgtacgtta aaagaaacag
    20101 cagctcattt tggctacaat ccggtctatc ttagtaataa attgaaaatg tattttgatg
    20161 cttcctttca agaactggtt ctaaagaaaa aattcgactt tgcgttggaa ctgattcaag
    20221 aaacgagttt ttcgttggaa gaaatcgcta atcgtatcgg atataaacaa acgacttctt
    20281 tattcaaatt attcaagaag tattcaacat gtactcccaa tgaatggaga aataaacatt
    20341 aagaagaaaa tctttcacta aagcatatga aggaaaattg ttttaggtgc gattgctgtc
    20401 ttattatatc gttgcagcga atttttttgg atttattttc agttctaaac ttttggttgt
    20461 attatcaggg gatataggtg taaacatttg tcatttatct gttatgcgta ttcaggaatt
    20521 cgtttccatc aaaaattata ataacaacta ttataattaa catgttgata aaatgaataa
    20581 acaagactat acttttcatg aaagtaacaa atagttatga aaaggagctg tttattcatg
    20641 aaaaaaacaa gtcgtaaagt agtaatcgtc ggtacaggtt ttgttggaac aagtatcgct
    20701 tatgcaatga tcaaccaagg aatctctaat gaattagtat tgatcgatgt gaatcaagaa
    20761 aaagcagaag gggaagcatt ggacctttta gatggtatgg cttggggcga tgaaaatgtg
    20821 gctgtatggt caggtggcta tgaagaatgt aaagatgctg atatcgttgt tatcacagct
    20881 ggtatcaacc aaaaacctgg tcaatctcgt ttagatttag ttaaaacaaa tgcgtcaatc
    20941 atgcgccaaa tcgtaaaaga aatcatggga tcaggattcg acggtattat cgttgttgct
    21001 tcaaacccag tagatatttt gacatatatc gcttggaacg aatctggtct gccaacatca
    21061 cgtgttatcg gtactggaac aacattggat acaactcgtt tccgtaaaga aatcgcattg
    21121 aaattaaaag ttgacccacg cagcgtccac ggctatatct taggtgaaca tggtgattca
    21181 gaagttgctg catggtcaca tacaactgtc ggtggtaaac cagtgtttga aattgtagaa
    21241 aaagaccatc gtattgccca agatgaatta gatgtgattg ctgataaagt ccgtaatgct
    21301 gcttatgaga tcattgatcg taaaaaagca acttactatg gtattggtat gagtactgca
    21361 cgtatcgtaa aagctatctt gaataacgaa caagctgtat tgcctgtatc tgcttattta
    21421 actggtgaat acgacgaaaa agatatcttt acaggtgtgc catcaatcgt tgacgaaaat
    21481 ggtgttcgtg aagttgttga attatcaatc aacgaagaag aaaaagcaat gttcagcaaa
    21541 tcaacaagtg ctttaagaga agtattgaac actgtattat aagaaacaat gaatatgaac
    21601 taa
//
LOCUS       DAZKMK010000047        20730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000047 DAZKMK010000000
VERSION     DAZKMK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.047"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..992)
                     /locus_tag="ACG8V1_001632"
     CDS             complement(105..992)
                     /locus_tag="ACG8V1_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0329727.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            1177..2016
                     /locus_tag="ACG8V1_001633"
     CDS             1177..2016
                     /locus_tag="ACG8V1_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0329728.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2212..3186
                     /gene="sgrA"
                     /locus_tag="ACG8V1_001634"
     CDS             2212..3186
                     /gene="sgrA"
                     /locus_tag="ACG8V1_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0329729.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3484..3840
                     /locus_tag="ACG8V1_001635"
     CDS             3484..3840
                     /locus_tag="ACG8V1_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329730.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            3972..5000
                     /locus_tag="ACG8V1_001636"
     CDS             3972..5000
                     /locus_tag="ACG8V1_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329731.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(5026..5466)
                     /locus_tag="ACG8V1_001637"
     CDS             complement(5026..5466)
                     /locus_tag="ACG8V1_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0329732.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5626..6132
                     /locus_tag="ACG8V1_001638"
     CDS             5626..6132
                     /locus_tag="ACG8V1_001638"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0329733.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6300..8213
                     /locus_tag="ACG8V1_001639"
     CDS             6300..8213
                     /locus_tag="ACG8V1_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0329734.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            8218..9219
                     /locus_tag="ACG8V1_001640"
     CDS             8218..9219
                     /locus_tag="ACG8V1_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0329735.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9421..9627)
                     /locus_tag="ACG8V1_001641"
     CDS             complement(9421..9627)
                     /locus_tag="ACG8V1_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0329736.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10273..11121)
                     /locus_tag="ACG8V1_001642"
     CDS             complement(10273..11121)
                     /locus_tag="ACG8V1_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0329737.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11285..12187
                     /gene="gnd"
                     /locus_tag="ACG8V1_001643"
     CDS             11285..12187
                     /gene="gnd"
                     /locus_tag="ACG8V1_001643"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0329738.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            12189..13190
                     /locus_tag="ACG8V1_001644"
     CDS             12189..13190
                     /locus_tag="ACG8V1_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0329739.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13187..13471
                     /locus_tag="ACG8V1_001645"
     CDS             13187..13471
                     /locus_tag="ACG8V1_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0329740.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13504..14301
                     /locus_tag="ACG8V1_001646"
     CDS             13504..14301
                     /locus_tag="ACG8V1_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0329741.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14315..15148
                     /locus_tag="ACG8V1_001647"
     CDS             14315..15148
                     /locus_tag="ACG8V1_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0329742.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15166..16023
                     /locus_tag="ACG8V1_001648"
     CDS             15166..16023
                     /locus_tag="ACG8V1_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0329743.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            16045..16554
                     /locus_tag="ACG8V1_001649"
     CDS             16045..16554
                     /locus_tag="ACG8V1_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0329744.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            16591..16992
                     /locus_tag="ACG8V1_001650"
     CDS             16591..16992
                     /locus_tag="ACG8V1_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329745.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            17104..17457
                     /locus_tag="ACG8V1_001651"
     CDS             17104..17457
                     /locus_tag="ACG8V1_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329746.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17491..17934
                     /locus_tag="ACG8V1_001652"
     CDS             17491..17934
                     /locus_tag="ACG8V1_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0329747.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            17951..19036
                     /gene="uxuA"
                     /locus_tag="ACG8V1_001653"
     CDS             17951..19036
                     /gene="uxuA"
                     /locus_tag="ACG8V1_001653"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0329748.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            19029..19742
                     /locus_tag="ACG8V1_001654"
     CDS             19029..19742
                     /locus_tag="ACG8V1_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0329749.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(19949..20098)
                     /locus_tag="ACG8V1_001655"
     CDS             complement(19949..20098)
                     /locus_tag="ACG8V1_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329750.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            20494..20694
                     /locus_tag="ACG8V1_001656"
     CDS             20494..20694
                     /locus_tag="ACG8V1_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0329751.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7087 a         3203 c         4310 g         6130 t
ORIGIN      
        1 gccccgatga agttgtggca aaagtggttt gcctcaaaaa taagaaggaa tttccaaaaa
       61 tcacttctca aattttgcca catccccaat tgggtcaaat ttgtttattt gaatatattt
      121 tttacataag gatctttaat agttacattg tcttttttca tcactttacg gattgtttta
      181 tccatgaatt ccgtatcaag agtttttgct tcggtagcaa ttttttctag ctcagatttg
      241 tatttgtctt tattagaacc tttatcctgt ttttttacca tttttactag ataatagctt
      301 tgctgataag tcgttggatc tgttacagga atgatatcag aaacttcccc gttttttagt
      361 ttaaatgctg ctttttttac ttcagtagga atctctggat tggttgaatc aaaattcatt
      421 tttccatcag tttcctttaa tgttttattt tttccataag cttgaacaag cttggagaaa
      481 ttttctcctt catttgcttc tttttttgct ttatctgctt cttctttagt cgaaaataga
      541 atgatctgtg tactaacttc tggatagaat tctttccaag cagtgtctag atctttatct
      601 gttaatttta cattggcttt caaaccgtat tggaaagcca gcatctgttt aaagagtttc
      661 ttatatgtct cttctgtttg gccaactgct tttaattgtt ccttgtagtt tggaccgagt
      721 tttttgattt gttcattata ttcttttgtt acttcctttg aagaaatctc ttttccgtat
      781 acatcatcaa atgctttgaa gacaatcatt ttttttaata gtgtttgatt atttgattta
      841 acttttgggt tatcataaaa atcctgaact gtaagtgttc cacctttaaa cgttgcaatc
      901 gtttcattgg acttgctaca acctgagaat agtaaaattg cacatgctga aaggcacata
      961 attagtagtt gttttttcaa aaaatttccc atcctttcct tattaaaaac agtctaacag
     1021 aagaattagg gagacagtct ttcattttcg taaggttgct gtagatattc ttagttcgta
     1081 aaaatctatt ctctctatga tggagaacat ttacatagtt ttcacgagac aagaaagaaa
     1141 agggttgtta aacttaatat aaaggaggga tgaaagatga tcaggattat tcggttgatt
     1201 ggatctgttt taatagtctt agttggtttt gttttaggaa ttcttgtcaa taatgcactt
     1261 gcggatatgt ttattggaaa tagtgaatgg attggagcaa ttgccggtac ggtcggacat
     1321 ttagctgttt ttttactagc tttatttctt gctagtaagg tcgaaggaaa aagaatagaa
     1381 gattatggtg tttctgttcg aggaaaagac tgggggtatt tagcgggagg tctcgttgtt
     1441 ggaattggag tgtttctgct tattactagt ccattatatt tgacgggggc gtatcggtta
     1501 gattcaggaa acgcaaacat tgtgccgctg ataacgagtt ttattttatt tattgcagtg
     1561 ggggcatcag aagagctttt attccgtggc ttttttcagc atcagatgct tagatttggt
     1621 ccgttaatag caatgattgg atctgctgca ttatttgctc ttttgcacgg attgaatcct
     1681 aacatgacat ttctggcagt tttcaatatc tttttagcag gttgcttttt cagcgcactt
     1741 atctacagta caggaagttt atttacggct attggtgctc atattacttg gaattggaca
     1801 caaggagcta ttttgggtat accagtcagc ggaacgcaag aacaaggttt tttctccact
     1861 ctgatccaat cgcaaaatac aataattact gggggaaact ttggggctga agcatctata
     1921 tcaactacat tattcttgtt agtcttgatt gttggtttgt tactctatgc atataaaaca
     1981 aagcggatga agaaatacac tgctgtttat caataaaaga tgaagaaagc aaggctgata
     2041 tcggtcttgc ttttttaagt ctgtcattga ttaaacgaaa aagtaaacaa attcaagcga
     2101 atacacgaca tcttttgtta gattttcaca actgactgtc gtgttctcat cattttcatt
     2161 ttatataatt cctttatcaa atagaaggta attatatgga gagtgataga aatgaagaaa
     2221 acagcgaccg tattatttgt tattcttggg ctgagtataa ttgcagcaat gattggaatc
     2281 agtacttctg caaatgaacg ggcaaatgag aatctttcat ttactgtgaa aactgatcgg
     2341 attgtttatg acatgacaca acaagtcatt actattccag tcaaaccaaa caaaagcgtg
     2401 aatgctagtg atgtacatgc tgttctaaca tatggttggg atggtaatgg aagttcagaa
     2461 aaagttattg gggaagttta tttaaaagac gtacaatgga ccgctggtat tgagtataca
     2521 ataatgattt ctgcagaact atcgattgat gagatcaaga gtaaagataa agttgatttg
     2581 attgtttttt atgatggaca gatgacgatc acggagaatt tgaaaccaag cagttggaca
     2641 gttgttggac cgccaagcag tacggatagc acaagtagtg aaagcagtac agaaaacacg
     2701 agcggtgaaa gcagtacaga gagtacgagc agtgaaagta gcacagaaaa cacgagcagc
     2761 gaaagcagta cggagagtac gagtagtgaa agtagcacag gaagcacgag cagcgaaagc
     2821 agtacggaga gtacgagtag tgaaagtagc acaggaagca tgagtagtga gagtagcaca
     2881 ggaagcacga gcagcgaaag cagcacagga aaagtaaata atgaacttga tttggtacct
     2941 atcataccaa ctaaggaaca aaagagaaat gcagggagca acctaaaaag tgtgctaaaa
     3001 cagcctgatt ttgtttctcg tggaattgat catcaagaaa aagcgaaaga aacagataat
     3061 agaaaaagcg atttaccaga aactggtagt agaaccttga acaagctaat tacatggatg
     3121 ggtgtgttac tcattttgat tgtaggagca agctattttc gttctctctt ccatcgagtg
     3181 aaataaagaa taaaagcaac agatctaaaa ttgaataaaa cgatagattt ttgagtgaat
     3241 atttcattcg agagtgctgt attgcctggc gaagttgtag ctttgccaga tctatttttg
     3301 tttactaaca tatttgagaa acaggaaata acaggaacac aaatggaaaa taagagcaag
     3361 aataattgac aatgcacact gcgcatggta gtatctctga aaaatcagta acgaatctat
     3421 ttgtacgtta ttcatgtagc tgtcggaata agtctaaata atagataaaa aggagtatgt
     3481 actttgtttg aagaatttat tgatatcaat gaaaggcaag tatatcaatt tttgaactat
     3541 tgttatgaaa gagacgagaa attatatgtg gtgaaggata tcgctttaga tctaaactac
     3601 accctggcta agatgaattc ggtgattcaa caagcagaat cgttttgcga aaggtatcca
     3661 gaatataaat tatcttttct ttcagaaaac aagatgataa aagtcgagtt ttcttctcag
     3721 tttttactga gtaaagttta ttctatactt ttagagggaa cgattggtta tattttactg
     3781 gattcacttt acaaaggtac ttatcaatcc ttagaaaatc ttagtcaaaa aattatataa
     3841 gtttgcgtac gattcaaaga aaattaagag aacttaatac tattttgaaa aactatcatt
     3901 tatcttgcag cttgaaaaaa ggtaatccga tagaaggaga agaataccgt atccgatact
     3961 tttttcactt gatgtattgg cagtcattcg acgaaaatca ttctgatata ttatcaagta
     4021 taaaaaataa acgtcaacta ttagaaaaat ttgcgaaaca tgcgccatat acgcgtagta
     4081 tagatagaaa gaaatggatc agcctactaa gaatttcgtt gcaacgaatg aaacaaggcc
     4141 actcgattac gtatgtccca gaagagatga acaaatttgt gcatccattg ataacaaaag
     4201 aagagtttac gcattacata ctagagcctt tttttgaagc aaacttctgg tcgatgaatc
     4261 acatcactcc aacagagcaa gcttaccttt attttatgtt tggtgtaatg aacacctatt
     4321 tggaagaaga tttgttggat actccagata aagtacaaga aagttggggc tggattgaat
     4381 cgtctattgt agaaaatttt gaaaaaaact tcaagatttg ttttacaact tcagaaaaaa
     4441 attatctata tgtcaatttg gcaatgatcc atttgtattc aagggttttt gggacaaaaa
     4501 agaaggtgga tgcttttgga aaatcagcag aagacagaga ttttcagcat atatttccag
     4561 tgatgtatcc aatcatgcaa cagtttttta gtggggtagc tcgcagaatt ccagagctta
     4621 atacttatta tgaaaagaac agacggttga tttttcaata ctgtatgtta gtcagggata
     4681 tttttataaa acacgaacaa cctgtcatat tctatattca atcaaagtat ggtaaaacac
     4741 aggaattatg ggcaaagaaa aggatattat ctatgactga acgcccaata aaatatagtg
     4801 catccgctga tcagttacct gatattgtca ttagcgatta tcctatcgac aaaaaggcat
     4861 atgatcaaac gaatacacag atattttact ggaatggaca acctacaaaa aatgattggg
     4921 cgcgtctgaa agctgaaatc ataaaaatca ggaatgagaa aaatagatcc atattggaaa
     4981 aattggattt cgttaattaa gaaaagcaga ttgtttatgc caaaattaag acattgcaaa
     5041 aagattattt tctttaaaat cgacaagatg accagaaagc aagagttcgt tgagctcttg
     5101 ctttctttta gttttagaaa ggaacatcct gattttatct tcgctaaatc cagtcaaaag
     5161 ttttgcagat tggtcgtaga taatcggttt tttcaataaa gaaggggatg tacgaatctc
     5221 aatatatagt tcactcaaac tgagttcatc tattctgtga acaagatttt tataactaat
     5281 agaatgagta gagagcattt cggtcacgcc gttgtttgtc aattttagta agtggataaa
     5341 ttcatttttg gttagtggtt gacgaattaa gttctgttcg ataaacggta tatcatggac
     5401 gataagccaa cgttttgcgc gtcttgaaga cgagcagcca gaacctgtat acaatttaat
     5461 catcatacat tcctccaata gctaagtatt tatgaaataa taacatgaag aaatagtcga
     5521 aaagtgccat ttagaggtgt aaaataagaa aatgttgtca cgtataatgg aactagctag
     5581 ataagaaata ctattaaatc aagaaaaaag aaagaaggca caagtatgtc aaacacagcc
     5641 gacgcaatag tagtgatcga tttacaaaaa ggggtatgtt ttgattcttc gccgatacac
     5701 gaattagaaa cagtgacagc attagtgaat aaaagaatca aaacatacga aacgattggg
     5761 aaaccagtta ttttcgttca acacgaagat gctcaattag tgccacaaac aaaaccatgg
     5821 gagctattgt cggaattatc tattcctgag cacagttatt ttgtgagaaa aacccatgcg
     5881 aattcatttt ataagactaa tttgagtgtt ttattaactg aattggactg tcattcgatc
     5941 gaattatgtg gggcacaagc agagtattgt atagatacga ctgtgaagtt tgctcatgga
     6001 ttaggatacc agcttatgat gtgtagagga gcaacgacaa cttatgataa tccttttatg
     6061 aaagcaaagg aaacaatcgc tttttacgag aaaatttggg ataaacgatt tctgacattt
     6121 ttggagcaat aagttgtttc ttcgtttaaa aaaagcagaa tggatactgg tttatccatt
     6181 ctgctttatt acgtaaaatg agaaaataat cctgttctat ttttatggga taaaccattt
     6241 attttttgta taaaaaatgt ttgcgtttac atataattct cctagaaaca taaaaaggag
     6301 tgaatagctt gagaacaaaa caagtattaa agggagcgtt ggtcatctta tttgctgtaa
     6361 gtacaagcgt cccgcaatgg acagtatatg gagagagcat ttcttccgaa tcggaatctc
     6421 gggaggtaga aaccacagaa actactgatg ctggaaatga aacgaaatcg agtacttcac
     6481 aaacagaaac aaatactgaa acaacggttg agacatctgt tactagcaca gatcaagaaa
     6541 cgaccgctac cacagataac caacaaacag aagaagcaac agctacaaaa atgtcaaacg
     6601 acgaatcctc ttctgctaaa gtaagtgaca aagagaatgg gacagaaaat gatgatatcg
     6661 gtaaaggtga aaatttcaat ggtaaagaat atttgaatta cttcggtaaa cagatgacag
     6721 aaatacttaa tggaccaaca gaagacatca agtcaaaatt aatagagtta ttagtcactg
     6781 agcttgctgt taatgattat ggtgtagata aagagattat caaacatctg attgctactt
     6841 atctattgta cgatatcgct tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta
     6901 cagatgatgg gacaacgcct acagaaaaca caggcttcta tccaaataca aaacaacttc
     6961 atcaaactgt actaaacaaa caagatggca aaatgatgga tgtatatgca tattatgtcg
     7021 atcaaggttc agataaaaca gtcatgatcc atggcggatt tcgagggaat tggaataacg
     7081 ggatcgttac agaagaatac aatgattttt ataaggccgg gtataattta ttatttgttg
     7141 attccagagc gactggtaat agcggaggcg attatgtgac ctatggacaa tatgagtcag
     7201 atgacgtcct ttactggata aatcaagaag ttcgagagcg gccatctcaa aagattttac
     7261 tttatggtgg ttcgatggga gcagctacga tgatgtctgt attagcgaaa gatattcctg
     7321 tcaatgtcaa aggaattatt gaaaattgcg gatttgcatc aattgatgaa caactacgtt
     7381 ttacgtacag ccagacagta gcccccgcac taccggatgc cataaaaaat cagttagaca
     7441 tcattgggga tcaagagcat gaagatttat ttatgggcct tttgaaacaa tactattttg
     7501 atcaggagat gcatttggat acaaaagctg ctctacctac gattggaatg tcagattcat
     7561 tgcctaaatt gattatccat ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa
     7621 aattatatga gttatcagga gggtataaag atcttttatt agtagaagga gcagggcatg
     7681 gaaaggcaca ggaagttgac catgctgcct acacgaaaca tgtaacggac tttttgaaag
     7741 tagtgttcca tgatcagata aacgtaaagt atgtggatga aaacaataaa agtctactca
     7801 aagaccagga cgaaatccag ctttacggag cttatggaga aaattatatg acagagcaaa
     7861 agacattcga aggatacgaa ttagcaaatg ttgaaggacc gactgaaggg atttttaatg
     7921 agacgatacc aactattatt ttctattaca aaaaaatccc agtagctcca ccaaaaaagc
     7981 aagatccgac agagaatgca gacaataagg gaaagaatga acctcgagag gatcaatcaa
     8041 gtgatgatac gcaaccgatt cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa
     8101 aaagaatgtt gccgaaaact ggagaaaaga aacattcgct tctcatttat tcaggtggat
     8161 ttttatctgt tagcacgatt atcacatgga tttggcgaag aaagagaggg taaatgaatg
     8221 aaatggttca agcgaatagt gattgcagta ccagttatca tcgtagttat tttagtcact
     8281 gtatttttga ttaatcaaat cacaccaaag ccgattagct tgcttgttag aaaacaattt
     8341 gacacaagtg aaaaagaaca agtatatgcc cgaccagatg atttccagca aaaaatcgaa
     8401 caaatcgata taaaaaaaga aatatcctat ccttcaaagt acaaaaataa taatatggat
     8461 atctacacac cgaaagtgga aaagaaaaaa ttaccgattc ttttctggat gcatggaggc
     8521 gcatatgtag gaggggataa aaatgattgt cgtgattatt tggaatatct atgttcagac
     8581 acgcagcaaa tcatcgtaaa tatagattat gaacgatcac cggaagcaaa acatcctaca
     8641 ccagttatcc aattgaacga agccattcaa gcagctaaaa aagagataaa agatcaagca
     8701 gattggtcga atatttcgat tggtggaaat tcggcaggtg cacaaattag tggagaatat
     8761 ttgttagcac ttcaaaatga agaaattaaa aaagctgatc atcttgatcc gactttggag
     8821 aacaagcaga ttacgaagtt tatttctttg tctggtctgt tggatccttc tgcacttact
     8881 caagtaagtg ataaaatctc tagctttctt tatgaaaaat gtggatgggc ttactttgat
     8941 aataaggact ttgaacaatc agagaaggtc aaaagtttag ccttggtcag acatgcggat
     9001 cgttggacgc aaaacacatt cttaacggat ggaaatacaa atacgtttac gaaacaaatg
     9061 gaagaagtaa cttctgtatt tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg
     9121 gagaaaaatg ctgttttgaa ccatgaatat cagtttgatt tcagcaataa acaagcgcaa
     9181 gaaacatatc aaaagttagt aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg
     9241 ttgtaatctg tcacttagct ccaagtcaca aggagcaatt tcaataaaca agcttttcat
     9301 ggtttatgaa tattgtaact tgtgactgag gagttagaga tggcaagggg caccttttat
     9361 tcgaaatttg ggaatatcac acgattattg tcacgatctc ttttacgtaa attttataag
     9421 tcagatttgt tgttttgatc gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag
     9481 aagggacaaa aagccgagtg gtaagcaaaa gaatgctggt tccactaata ttccgttgtt
     9541 attgatataa ggctgggaac aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa
     9601 aagcaaagaa atatggatag ctttcataag cagcacctcc ttttttataa ttgtatgata
     9661 aatagattaa gaatctataa atgtgacaag tttgttagga tgtgaataaa atgatcatct
     9721 agcgaacttg aaacaaagat ggctaatgat aagcaattaa tgattcagca atcagatagg
     9781 tacaaagttt accatcatac gtctagacca ataatagaaa atatgggaat ataatagaaa
     9841 agatttagga tacccccaaa agaatttccc caaaaattcg atgtatccta aactttttta
     9901 ttttacataa caatcatatc aactgtgaaa taaaattaca aatgataacc taaaatgagg
     9961 ccaaaagcca gtgttatcaa ggaaatactt aattcaccaa aatttttttt aattttttag
    10021 aaaataagaa acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta
    10081 actttgttat cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat
    10141 gcgatgacat acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca
    10201 aaaaagccac taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt
    10261 tctgttatgg tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg
    10321 acgtgattct ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag
    10381 ttgagaaata cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat
    10441 aaaaacgaca tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt
    10501 ttttgctcca gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt
    10561 gtgagaaatc aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat
    10621 atggtaatcc gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc
    10681 agttgctaca atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat
    10741 aatagtagcc gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt
    10801 tgaaatacta gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat
    10861 attttcgtgg atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa
    10921 atctttaaat ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat
    10981 gcccagttca cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa
    11041 aatataatcg gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg
    11101 tagtcttatg gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc
    11161 cgttctcatt aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt
    11221 tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc
    11281 tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt
    11341 aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc
    11401 aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca
    11461 gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa
    11521 actttttcca aaactcggac caggagacat tattattgaa ggcggaaact cttattataa
    11581 agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg
    11641 gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa
    11701 agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct
    11761 gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata
    11821 tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt
    11881 tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat
    11941 ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt
    12001 ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt
    12061 tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc
    12121 agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg
    12181 tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct
    12241 ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc
    12301 aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa
    12361 gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt
    12421 cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa
    12481 tcgtatctat catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta
    12541 atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat
    12601 gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg
    12661 gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg
    12721 ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag
    12781 tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag
    12841 aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc
    12901 tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa
    12961 gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc
    13021 atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg
    13081 aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc
    13141 ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat
    13201 ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat
    13261 ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg
    13321 aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca
    13381 atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac
    13441 aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat
    13501 ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct
    13561 agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa
    13621 gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg
    13681 attgattccc ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat
    13741 gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca
    13801 caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa
    13861 gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct
    13921 gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct
    13981 atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa
    14041 gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag
    14101 ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt
    14161 ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg
    14221 catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa
    14281 ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag
    14341 tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac
    14401 gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga
    14461 agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc
    14521 tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc
    14581 attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct
    14641 cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga
    14701 caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac
    14761 ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc
    14821 attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt
    14881 agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa
    14941 agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga
    15001 tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc
    15061 gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga
    15121 cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg
    15181 gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg
    15241 agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta
    15301 ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca
    15361 gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt
    15421 actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag
    15481 atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca
    15541 ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta
    15601 ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg
    15661 tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct
    15721 acaggcctaa agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg
    15781 ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt
    15841 gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata
    15901 ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc
    15961 acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta
    16021 taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt
    16081 ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt
    16141 gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga
    16201 agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat
    16261 gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac
    16321 gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa
    16381 aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa
    16441 aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca
    16501 tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat
    16561 gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg
    16621 gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt
    16681 ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac
    16741 gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa
    16801 gttatctgta cttattacga tacacccgtt catgcattag gaagttattt catgtttgat
    16861 tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc
    16921 agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg
    16981 ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga
    17041 aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta
    17101 acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat
    17161 gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct
    17221 attctttttt atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat
    17281 ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat
    17341 tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta
    17401 cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta
    17461 aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt
    17521 aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt
    17581 gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa
    17641 aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca
    17701 agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc
    17761 acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg
    17821 cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta
    17881 actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa
    17941 ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt
    18001 aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat
    18061 gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca
    18121 aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac
    18181 agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg
    18241 tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt
    18301 gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca
    18361 gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc
    18421 gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat
    18481 gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg
    18541 cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg
    18601 ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc
    18661 tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt
    18721 agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga
    18781 gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga
    18841 tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga
    18901 tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc
    18961 aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg
    19021 agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa
    19081 ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc
    19141 gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg
    19201 gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata
    19261 aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt
    19321 tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa
    19381 aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag
    19441 gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa
    19501 tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag
    19561 caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc
    19621 ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact
    19681 atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct
    19741 aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag
    19801 atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag
    19861 aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga
    19921 gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt
    19981 gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata
    20041 gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag
    20101 ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc
    20161 atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag
    20221 ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact
    20281 ttttcttcga ttcgaagaca atagaagaca atgaaattta tatttcattg cattatcctt
    20341 gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag
    20401 cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa
    20461 cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc
    20521 aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat
    20581 ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac
    20641 gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa
    20701 ttgactttta accgtctttt cgaaagaaaa
//
LOCUS       DAZKMK010000048        20293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000048 DAZKMK010000000
VERSION     DAZKMK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.048"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..1666)
                     /locus_tag="ACG8V1_001657"
     CDS             complement(107..1666)
                     /locus_tag="ACG8V1_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0329752.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1821..4055
                     /gene="pcrA"
                     /locus_tag="ACG8V1_001658"
     CDS             1821..4055
                     /gene="pcrA"
                     /locus_tag="ACG8V1_001658"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0329753.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4072..6108
                     /gene="ligA"
                     /locus_tag="ACG8V1_001659"
     CDS             4072..6108
                     /gene="ligA"
                     /locus_tag="ACG8V1_001659"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0329754.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6214..6519
                     /gene="gatC"
                     /locus_tag="ACG8V1_001660"
     CDS             6214..6519
                     /gene="gatC"
                     /locus_tag="ACG8V1_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0329755.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6519..7991
                     /gene="gatA"
                     /locus_tag="ACG8V1_001661"
     CDS             6519..7991
                     /gene="gatA"
                     /locus_tag="ACG8V1_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0329756.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7988..9418
                     /gene="gatB"
                     /locus_tag="ACG8V1_001662"
     CDS             7988..9418
                     /gene="gatB"
                     /locus_tag="ACG8V1_001662"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0329757.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9428..10483
                     /locus_tag="ACG8V1_001663"
     CDS             9428..10483
                     /locus_tag="ACG8V1_001663"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0329758.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10594..11967
                     /gene="rlmD"
                     /locus_tag="ACG8V1_001664"
     CDS             10594..11967
                     /gene="rlmD"
                     /locus_tag="ACG8V1_001664"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0329759.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11970..12098
                     /locus_tag="ACG8V1_001665"
     CDS             11970..12098
                     /locus_tag="ACG8V1_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0329760.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12538..12726
                     /locus_tag="ACG8V1_001666"
     CDS             12538..12726
                     /locus_tag="ACG8V1_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0329761.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12804..13817
                     /locus_tag="ACG8V1_001667"
     CDS             12804..13817
                     /locus_tag="ACG8V1_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0329762.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13884..14918)
                     /locus_tag="ACG8V1_001668"
     CDS             complement(13884..14918)
                     /locus_tag="ACG8V1_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0329763.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15033..17258
                     /locus_tag="ACG8V1_001669"
     CDS             15033..17258
                     /locus_tag="ACG8V1_001669"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0329764.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17276..19159
                     /locus_tag="ACG8V1_001670"
     CDS             17276..19159
                     /locus_tag="ACG8V1_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0329765.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19140..20114
                     /locus_tag="ACG8V1_001671"
     CDS             19140..20114
                     /locus_tag="ACG8V1_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0329766.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6841 a         3720 c         4351 g         5381 t
ORIGIN      
        1 aaaaaagaag aggctgggtt ttcccagcct cttctttttt tatagaatca tatagaatca
       61 agtagtgtag atgaatagtt gttgtaaaat agtttttcca tgttcgttaa aatgattgct
      121 tcaaatttgt gatagaagat ttagcctcga tttcctgctt gattcttggc aagtttgctt
      181 catcagtcaa gataaccaaa atatctccta cattcatgat cgtatcaccg tgagtcaaga
      241 tttcctgatt tcctctgcgt atagagatta gcagcatatt tttaggccaa ttgaagtctc
      301 gtaccattaa tccgtctaaa gaactctctg ccgttactgg aaaatcaaaa gttgtccttg
      361 caccttttag tgaggcggtc tgagtatttc caagtagacg ttccaataat gattcgtaaa
      421 ttggattacc gccgagaagg tcattgacga tataagcaac aagagcagcc aatcctaaag
      481 gcatcagatg attaagagag ccaaccattt ctgtcactaa aataattgct gtcagcggtg
      541 cttttccaat cgctgtgaaa taaccagcca tggcaataac gacaaaatcc ttgatgtaga
      601 ttggatccat cccgaaatca accataatgt ttccataaaa gctaccgatt aaagccccca
      661 atgtcaatat cggaaggaaa atcccccctg gaagaccccc accgtaagaa atcatggaaa
      721 aaacgaatcg aagaacaaac aaaccgataa tcataatgat tggcaaggaa gcatgtccaa
      781 aagctaaaac gatttgattg cctccgccta gagtactagg ccataaataa ccaattggca
      841 aaatcagcaa gaaaggaacg aacccgtgaa aataagctgg taaaggcaat cggttgtaaa
      901 attttggcaa agccagcaag acattctgat aaataaaacc taagatgcct agaagtactc
      961 ctaagatcac taaggaacca taatatttca acggcaaaga aggaaggtca cctagatata
     1021 aaacaggtct caagccgaaa aagtaaagag agacgaagtt agcagtgatt gctgaagcaa
     1081 atgatgtcag ccaaacaagt ggagaaaagg agtgatggat ttcttctaaa acaaagagta
     1141 atcctgcgat aggagcattg aatgctgctg caagtccgct acttgcaccg ctagaaatta
     1201 aaatcttttc ttctgaagat ggactccgaa acaattgtcc agctccttgt ccaatggatg
     1261 cgccaagctg tatagaaggt ccttctctac ctagaaaaag gcccgcacca actgaaagta
     1321 caccgccaat gaactttttc cataaaacag agaaccagtt cattgataat tccccgcgaa
     1381 gctgtcccac tacttgtgga atcccgcttc ccttgatatc cggttcattt ttgaccagat
     1441 aacccacgaa aaatgctgca aggatgcagc aaatagctag gccaattaag taaatcggct
     1501 gttcccgact catttcatag atacttcgaa ctaaatcgct tagcttttcg atagataatc
     1561 gaaacaggct aacgacgatt cctgtcagca gtccaacaaa tattcctttt agaataaaat
     1621 atatctgagt cttatctaat ttcttgattt ttcctttttt ttccattcaa acgcctcgct
     1681 ttcttttaat ttagcgcgaa atcaacgaat tgacaaggaa aaatccataa aaagaaacaa
     1741 atgattttga actagctagt ttaatgaaag ccaagaaaat gctataatta acaagattga
     1801 taaaagaaag gatgttgtta atgtcatcca aagctgaatt attaaacggg atgaaccccc
     1861 gtcagaaaga agctgttctt cacactgacg gaccattact gctaatggca ggtgcaggaa
     1921 gtggaaagac gagagtgctg acacaccgca ttgcctattt gattgaggaa aaagaagtca
     1981 atccttggaa catcctagcg attactttta ccaataaggc tgcaaaagaa atgaaagaac
     2041 gtgtcaatgc gattctcgca tctggaggcg aagatgtctg ggtatcgact tttcactcga
     2101 tgtgtgtgcg tattttaaga agagatgttg attttatcgg gtataaccgg aactttacaa
     2161 tcatcgattc ttctgaacag ctgaccttga tgaaacggat actaaaagaa ctgaacattg
     2221 atccgaaaaa atatgatcct cgaagtatat taggaacaat ctctcaagca aaaaacagcc
     2281 tgcagacccc tcaggatttt gcaaaaatgc aaggcagtta ttacgaagag attgcggcaa
     2341 aatgttatgc tgcttaccaa aaagagcttc aatataatca atgtatggac tttgacgacc
     2401 tgatcatgaa tacgattcga ttatttgaag aacatcctga ttcactcacg tattatcaaa
     2461 acaaattcca ttatattcat gtagatgagt accaagatac aaatcacgca cagtatacgc
     2521 tagtcaattt attggcaggt agatttagga acctgtgcgt cgtgggagat gcagatcaaa
     2581 gtatttacgg ctggcgcggt gcagatatgc agaacatctt ggattttgaa aaagattatc
     2641 cagatgctgc agtaattttg ctagaacaaa actatcgctc cacgaaaaac atccttagcg
     2701 cagcaaacca agtcattgaa aacaatagca atcgaaaacc aaaaaacttg tggacagaaa
     2761 acaaagaagg aaacaagatc acttactacc gtgcagataa tgaacgagac gaaacccgtt
     2821 ttatcgtaga ccggatgcaa gaagagattc gaagcaatca tcggaattac ggcgactttg
     2881 ccatcttgta ccggacgaac gcacaatctc gtgtaatgga agaaactctg ttgaaagcaa
     2941 atattcctta taaaatggtt ggagggcaca agttctacga tcgtaaagaa atcaaagata
     3001 ttttagcgta tttgaatgtg ctggtaaatc ctcaagattc aatcagtttt gagcgtatcg
     3061 ttaattcgcc caaacgaggc atcggaccag gatctatcga aaaactacgc agttttgctt
     3121 ctcttcatga atggccactg cttgaagcag cgcaaaatgt ggatctggca aatatcggtg
     3181 gaaaagcagg acagcaatta ggtgcatttg gagagatgat ccaagaagtc actcagatga
     3241 tcccatactt gacagtgaca gaattgacga aagaggtttt ggatcgtagc ggatatctag
     3301 aagacttaaa aatccaaaat acattagaag cacaagcacg catcgaaaac ttggaggaat
     3361 ttttaacggt cacacaagaa ttcgacaaac aattcgaaca gcaaaatgaa gaagacgctg
     3421 atgcaccaga agaaaaacta acggtcttct taaatgatct ggcacttgtt tccgacattg
     3481 acaatttaga agaagatgct tcccaagtga cattgatgac tttgcatgca gccaaaggat
     3541 tagaatttcc agttgtattc ctgattggtc tagaagaggg ggtttttcct ttatctcgag
     3601 cattgatgga agaaagcgaa ctggaggaag aacgccgtct cgcatatgtg gggatcacgc
     3661 gagcagaaga agcgttgtat ttaacaaatg ctttctccag aacattatat ggacggacgc
     3721 aatataaccg accttctcgt tttgtagaag aaatcgatca ggagttactg gaaatcgaag
     3781 gcatgcgtcc tacgcctaaa aaaacgcctg tattcgctaa aaaaacagca tatagctata
     3841 aacagccaga gacagcggtt gttccctcta aaagtgctac tggaggggaa aataacagct
     3901 ggaaacctgg agataaagtc aaacacaaaa aatggggtct aggaacagtc gtccgagtaa
     3961 gcgggacgtc gaaggatcta gaactagatg tggctttccc aagtcaagga gtaaaacgtc
     4021 ttttggctgc ctttgcgcca attgaaaaag cttagtagtg gagggtaaat gatggaacat
     4081 gaaatgacga ttgacgaagc tgctaaacga gcagaagaac ttcgtacaag actgaaccaa
     4141 tggtctcgcg aatactacgt agaagataaa ccaacagtgg aagactatgt ttatgataaa
     4201 gaatacgcag aattagtagc aatcgaggaa caatatcctg atttgatcac gtctgattct
     4261 cccactcaac gagtaggcgg aaaagtatta gaaggttttg aaaaagtcac tcatgatatt
     4321 ccgctttaca gtttgaacga cgttttcagc aaagaagagc tgatagcttt tgatcaacgt
     4381 gtacaaaaag cagtgggacg tgtagttgac tattgctgtg aactaaaaat agatggactt
     4441 tccgtttctt tgcgttatga agatggaaat ttcgtgagag gtgcgactcg cggggatgga
     4501 actgtaggag aaaacatcac tgaaaaccta aaaactgtcc ggtcggtccc aatcaaatta
     4561 aaagaaccaa tgaacatcga agtaagaggc gaatgtttca tgccaaaacg gtcatttgtc
     4621 caattgaacc aagacagaga agcagaggga aaagatatct ttgcgaatcc tcggaacgcc
     4681 gcagctggtt ctttgcgtca gttagattct aaaatcacag cgaaaagaaa tctggacact
     4741 tttttataca cagtagctga tttcggtcct atgcaagcaa aaacccagta tgatgcgcta
     4801 gaagagctag aaaaaattgg attccacacg aatcgggaaa aacgtctatg tcattcgatt
     4861 gatgaagtgt ggtcatacat tgaagaatat catgacaaac gtgtggattt gccctatgaa
     4921 atcgatggaa tcgtcatcaa agtgaatgaa ttctcgctac aagatcaact tggttttact
     4981 atcaaagcac cgagatgggc agcagcttac aagtttccgc cagaagaagt tgaaacgcta
     5041 atcgaaaaca tcgaatggac agtcggtcga acaggggtag taacgccaac agctatcatg
     5101 acaccagtcc gagtagcagg aacgacagta agccgtgcca gcctgcataa cggtgattat
     5161 attaagttga aggatatccg cttgaaagat acagttctga tttataaagc gggagacatc
     5221 atcccagaag tttcgcaagt cgtgttagat aaacgaccta aagacagcga agaataccaa
     5281 ttgcctacac attgtccagt ttgtggaagt gaacttgtcc atttggatga agaagtagcc
     5341 ttacggtgca tcaatcctaa atgccctgct caaatgaaag aaggtttgaa ccactttgtt
     5401 tcaagaaatg cgatgaatat cgatgggttg gggccgcgcg tactagaaca gatgtatgat
     5461 aaaaaactag tagcagatgt tgctgatctt tacaaattga cagaagaaga gttattgacg
     5521 ttggacaaaa taaaagaaaa atcagcaaat aatattttga cagcaatcga caacagcaaa
     5581 gataattcgg tcgagcggtt gattttcggg ttaggaatcc gacatgtcgg agcgaaagca
     5641 gcaaaaattt tagcagaaca tttcggtgat ctagaaacat tgagtaaaag tgactatgag
     5701 tcgattattg cgcttgatac gatcggtgat atcattgccg acagtgtagt cacttatttc
     5761 agtaacgaag aagtccatga attgatgaat gaattaaaac aagcaggcgt caatttcgaa
     5821 tacaaagggc ttcgaaatgc ccaattgcaa gaagtcgagt ctccttttaa agaaaaaaca
     5881 gtagtcctta ctggtaaact aacacgattt actcgtgaag aagcaaaaga gacgatcgaa
     5941 aatcttggcg ggaaagtgac tggtagcgta tcgaaaaaaa cggatatcgt ggtagcagga
     6001 gaagatgctg gcagcaagct gaccaaagca caagaattag gaatcgaagt ttggacagaa
     6061 gatcaaatgg cagatgcatt ggctaaaagt cgctcagtag aagaatgaaa aatgagtttt
     6121 ttcagacaaa tttcaataaa caatttcaga cacggcactc ttgtgttaaa atagagagca
     6181 aagtttttaa ctcgtttaga aagaaggggt agtatggcga ttagtgaaga acaagtgaaa
     6241 cacgttgcca aattagcaaa acttgcgttc gcagatgaag aacttgcaag ttttaccgac
     6301 caattaggta aaatcatcga tatggttgaa atgctggaag aagtagatac ggaaggtgtt
     6361 ccatttacgt cgaatgtggt tgaaacagta aacgtgatgc gacaagatcg ggcgcaagct
     6421 ggatggaacc gcgatgaatt attgaaaaat gtgccggaaa cagaagacgg ctttatcaaa
     6481 gtacccgcaa tcatcgataa tggggaggct ggcgcataat gacagaatta cacaatcgtt
     6541 ctctagaaga actgcatcat atgcttgtat cgaaagaaat cactgtacaa gatttgacaa
     6601 aaacaacatt tgaacgaatc aaagaaacag agcctgaggt cgaggcattc attacattga
     6661 acgaagaaaa agcattggaa caggcaaaag ctttagatga aaaagggatt gatgaaagca
     6721 acgtccttgc tggtattcca attggtatca aggataatat cgtaacaaaa gatatcttga
     6781 caaccgctgc atctaagatc ctttacaatt ttgaaccaat ttatgatgca acggtcatgg
     6841 ataaaatcta tcaagcagat atgatccctg ttggtaagct aaacatggat gaatttgcta
     6901 tgggtggaag tacagaaact tcttatttca agaaaacaaa aaatgcttgg gacccaacaa
     6961 aagtacctgg tggctcttct ggtggttctg cagcagcagt tgcagctgga caaattccag
     7021 tttctttagg tagtgatact gggggaagta tccgccaacc agcagctttt aacggcatcg
     7081 tagggatgaa acctacttat ggccgtgtct cacgttttgg tttgattgcc tttgcttcat
     7141 ctcttgacca aatcggacca atgacccgta cggtgaaaga taatgcatta gcattgacag
     7201 ctatcagcgg ttatgacgaa aaagacggta cttcttcagg cgtttcagtt cctgattttg
     7261 cagagggact gacaggcgac atcaaaggaa tgaagattgc tttacctaaa gaatacttag
     7321 gcgaaggtgt ggcccctggc gtaaaagaag ctgtattgaa agcagcagaa acattccgtt
     7381 cattaggtgc tgttgtggaa gaagtcagct tgcctcattc aaaatacggt gtggctgttt
     7441 attatatcat tgcctcatca gaagcaagtt ccaatttgca acgattcgat ggtatccgct
     7501 acggttatcg ctctgaaaat gtgaaatcat tagatgatgt atatgtcaac tcacgttcag
     7561 aaggattcgg tgaagaagta aaacgccgta tcatgttagg tacattctcc ttaagcgcgg
     7621 gttattatga cgcttacttc aaaaaagcag gacaagtccg tacattgatc aaacgagatt
     7681 ttgaaaaagt ctttgcagat tatgacttga tcatcgggcc atcttcacca acagttgcat
     7741 tcggaatcgg tgagaacatt aacgatccaa tcacaatgta catgagtgac atcttaacga
     7801 tccctgtcaa cttggctgga cttccaggta tgtctgtacc ggcaggttta tcagaaggat
     7861 taccagttgg attgcagatc atcggtaatt attttgatga aaaaacgatg tatcaagcgg
     7921 catatgcgtt tgaacaagca acagaattcc ataaacaaca accagctatt ttaggaggga
     7981 aagacgaatg aattttgaaa cagtcattgg actagaagtc cacgtcgagt taaaaaccaa
     8041 ctcaaaaatt ttctctccag cgccagctca ttttggtgca gaaccaaaca gcaatacaaa
     8101 cgtgatcgac tggggttatc caggcgtgct gccagtaatg aataaagctg cgctggaatt
     8161 cgggatgaaa gcagctttgg cattaaactg tgaaatctct aaagatactc attttgaccg
     8221 gaaaaattac ttttacccag ataatccaaa agcatatcaa atttcccaat ttgatcaacc
     8281 aatcggtcat gacggatgga tcgaaatcga agtagaaggt aaaaagaaaa aaatccggat
     8341 cgaacgtgtc catttagaag aagatgctgg aaaaaacatg catggtattg gcggttattc
     8401 ttacgttgat ttgaaccgac aaggcacgcc tttgatcgaa atcgtttctg aagctgatat
     8461 gcgttcacca gaagaagcat acgcttatct agaagcattg cgatcaatta tccaatttac
     8521 agaagtcagc gatgtgaaaa tggaagaagg ctctatgcgc tgtgatgcca atatttctct
     8581 tcgcccttat ggacaagaag agttcggtac gaaagcagaa ttgaaaaact tgaactcttt
     8641 atctttcgtg aaaaaaggtt tagcttttga agaaaaacgt caagcaaaag ttcttctttc
     8701 aggaggagaa atccaacaag aaacacgccg ttttgacgaa acaacgaata aaacagtttt
     8761 gatgcgtgtc aaagaagggt ctagtgatta tcgttacttc ccagagcctg atatcccgaa
     8821 attcgtgatc gatgacgaat ggattcaaaa agtaaaagct agtttgcctg agatgccagc
     8881 ttctcgtcgc gaacgctata tccgtgagtt tggacttcca gagtatgacg caatggtatt
     8941 gacacttaca aaagaaatgt ctgatttttt tgaagctgtt ttggaaaatg gtgcagatgc
     9001 caagcaagca tctaactggt tgatgggtga agtttctgct tacttgaaca gtgagaaact
     9061 ggaattagct gagacaaaat tgacacctga gaacttagca ggaatgattc gtttgatcgc
     9121 cgatggaacg atcagctcaa aaatcgccaa aaaagtcttc cgtgaattga tcacaaatgg
     9181 cggagaagcc caagaagtag ttgaaaaaaa tggttgggtg caattatctg atccgaacaa
     9241 actactgcca atcattaatg aaattttgga taacaaccaa caatctgtcg atgatttcaa
     9301 aaatggaaaa gatcgtgcgg ttggcttcct agtcggtcaa atcatgaaag caactaaagg
     9361 acaagcaaat cctggtgtcg tgaataagct acttaaagat gaattagcga aacgatagag
     9421 gctgaaaatg aaaaaagcac gcgtaattta taaccccacc tcgggaaaag aattattgaa
     9481 aaaaaatttg gcggatatcc tacaggcatt agaagaagca ggatttgaag ccagcgccta
     9541 tgcaactaca ccagaaccag attcagccaa gaatgaagca tgtagagtcg cagaactagg
     9601 ttttgatcta gtagtggcag ctggtggcga tggcacaatc aacgaagtgg ttaatggaat
     9661 cgctccgctt gaacaccgtc ctaaaatggc catcatccca gctggtacaa cgaacgacta
     9721 tgctcgagca ttaaaaattc ctagagataa tattaaagca gcagcagaag tgataaaaaa
     9781 acaccaaacc gtaaaaatgg acatcggcct ttctgataac agttacttca tcaatatcgc
     9841 tgcaggtggc tatttgacag aattgactta tgaagttcca tcagaactaa agagtatttt
     9901 tggttatctt gcttatcttg cgaaaggagc agagatgctc cctcgagtaa agccaatcaa
     9961 aatgcgcttg aagtatgatg aaggggaata tatcggaaat gcttcgatgt ttttcctagg
    10021 gctgacgaac tctgttggag gattcgaaca gatcgcgcca gatgccaaat tggatgatgg
    10081 gaagttttct ttaatcattg tgaagacagc aaatatcttt gagatcttgc atattgcagc
    10141 actcatgctg aatggcggtc gacatgtcga tgatccaaga ataatctata ccaaaacaag
    10201 cagtttgttc gttgaaacag aagatgatat caatcgacca gtaatgatca acttagatgg
    10261 tgaatacggc ggggaagcac cgatgcattt ccaaaatctt catcaacaca ttgagttctt
    10321 tgccaatacc gatcagattc ctgatgaagc aatcacaggt acagatgaag aagaattaga
    10381 ggttgtcagc aaagaattcg taaaagaagt cgaacgttta acggatgaag atatcgacgg
    10441 tgacggaaaa atagcagacg aaacacctaa tcaaaagaat taatcaaaag ccggtgctga
    10501 acgtagtaag gtcagtgccg gttttagttg tgtaattagc tgatttatag tagagttcag
    10561 taggattgaa aaataggctt aaaggagcaa aatatgacag aacagcaaat agtaaagaaa
    10621 aacgaacgat atacagtcga aatcgtcgac ttgagttatg aaggaatggg ggtagcaaaa
    10681 atcgatggtt atccattatt tatcgaaaat gcactccctg gagaagaagt agaaatacta
    10741 gtagtaaaag caggcaataa gtttggatat ggaaaagtcg aaacatttat tacacaatca
    10801 cctgatcgtc agcctgtaga aaacaatgca ttacttcgca taggtatcgc acctttagcc
    10861 catttgaagt atgaacaaca gcttgtcttc aaacaaaaac aagtagagca tgtaatggcg
    10921 aagattgcta agatgccaga agttcctgta cttccaacgg tcggaatggc agaaccattc
    10981 ggctaccgta ataaagcaca gatccctgtt cgccgtatcg atggacagtt agccaccggt
    11041 ttttacaaaa agaatagcca tgatctagtc cctatcgaag atttttatat ccaagatcct
    11101 gtcatcgatc aagcaatcaa agtagtcaga gatatcttgc aacggttcca agtacgagga
    11161 tataatgaag aaaaaaatga aggacagctt cgtcacatca ttatccgtcg tggtcattat
    11221 tcccatgaaa tgatggtcgt tttagttact cgtaaagaaa aattctttaa atccaaggaa
    11281 attgctgaag ctattcatga agaattgcca gaagtggttt cggtcatcca aaatatcaat
    11341 gaagaaaaaa caaatgtgat tttaggagac aaagaaaaag ttttatatgg cagagactat
    11401 atcgaagatc agctattagg aaaaacctat cgaatctcag caaagtcatt ttatcaagta
    11461 aatactcaac aagcagagta tctgtaccaa acagccatcg agtttgccga gttagataaa
    11521 aaagacattg tgatcgacgc ttattcagga ataggtacga ttggcttgtc tttagcagac
    11581 agagtggata aggtgtatgg gatggaagtt attccagaag caattgaaga tgcacagttc
    11641 aatgctttaa ataataaaat cgaaaatgtt cattatgaag tcggaaaagc agaaaaagtt
    11701 atgaaaaaat ggcaagaaaa agggatcaaa ccatctgtga tcgtagttga ccctccacga
    11761 aaaggattag atcatcattt tattgaatca gcaatcgaaa tgtcaccaga acgaatcgtc
    11821 tatatctcat gtaatccggc aacttttgca agagatgcta agttatttgc agaagcaggt
    11881 tatgccgtga agaaagttca gcctgtagat cttttcccgc aaacccatca tatcgaatta
    11941 gtcgcttcat tcgttaaagg agaataaaaa tggaccaaaa agaaaaaatg aaagaattga
    12001 tcaaaaagaa acaaggtgga ggccgatcca aacaaatgga aacacctaag aatgatcgta
    12061 aaaacatgag aaaaggccct aaaatcttta ataaatagac gtgctaaaga aagactaaga
    12121 agtgttaact tttgttattg tttcttagtc tttttagtta gtctacatta ttttggcgat
    12181 ttgccaataa ggactgttga gttgtgcaaa atcaaatctg ggtcagaatg aacgtcattc
    12241 tgtctcagat ttttcgttat ttcaatttga ttaactgatt gatcaaaaag atttttattt
    12301 tcatattctc aaatgattca acactaaata tacatgtttg acgcggaaat tattattcga
    12361 ttttggcggc atcagggggg atgacgacgt agcagaccca ttattttact caagaaaatg
    12421 tccgtgccta ttatttagaa tacgatagtg aacgtgctgg gagttttgtt cttttagaga
    12481 aagtctctga agataaatta gttgtcttag attttgactg taacgaaaaa tggcgaaatg
    12541 gaagagcacc agcaagtgat tgaccggatc tacgaagcga cacgatatct gcctttggat
    12601 cgtttgtgct taagtcctca atgtggattt gcttcaacag aagaagaaaa tgttttgttt
    12661 gaagaacaat ggaaaaaatt tcttttgtca aaagcattgc agaagaagtt tggggaaaat
    12721 cattaaatca tctaggagag cttgatagga aataacacta tggtataatc agaatgctta
    12781 atactaacta ctgagggagc cttatgaaaa agaaaattct taaatttatc ggatggttct
    12841 tcggaatcat tcttgggatc gcgtttgcaa tctttcttgc cttccagctt tcccccagac
    12901 ctggagcatt cttgatcaac catatgtttt caaatactgt tcaaatcact gataaaaaaa
    12961 cgttcgatca agcagaaaaa aacgtggcga aaaaaacaga tttgacgtat gagtccaagt
    13021 ataaagagaa tacttatgac atctactatc caaaaaacag caaggggcct gttcctgttc
    13081 tattttgggt acatggtgga ggattcgtcg gcggagataa atcgggtgtc aaagaatttg
    13141 cgacaaaatt agtttcagac gccaacatcg cagtggttgc gatgaattat gaattagcgc
    13201 ctgattcaga ataccctaat caagttcttc aagtgaatga actgatcaaa gatttattag
    13261 ctgaagaaaa agacgacaaa ttgctagaca tggaacaatt attttttggt ggagatagtg
    13321 caggttcaca aatcgcattg caatatgcag ctatacaaac gaatgatgcg tatgctaaac
    13381 aaatgaaaat caaacagcta cttccaaaag atagtctgaa aggaaccatt tcttattgcg
    13441 gacctgttga tttgaagcta acagcacagc agcattcgga tgatcgattt atgaagtttt
    13501 tcgtcaaaac cgttgcgtgg tcgttgattg gaacaaaaga ttggaaaacg agtgagcaat
    13561 tgaaagaagc tagtgtggct gaccatgttt ctaaggaatt tccgccaacg tatataactg
    13621 acggaaatgc atactcattc caggagcaag gtcaagcatt agaaaatcgg ttgacagaat
    13681 taaaggtacc cgtgcaatcc ttgttttata cagatacaaa aaaagagatt acccatgaat
    13741 accaatttga ttacacgcta aaagaatcgc aaaattgtta ccagcagaca ttggattttg
    13801 tgaataagta taagtaagag gttgtgaaaa agccgtttcg ttttgagtat gaagaaacga
    13861 agcggctttt cacaacctct ctttcaatac ctttttgttc ttgggataaa actttcccta
    13921 acaatcaatc gtgcaggata agtcaaaatg attggttcgt cccgtatacc gtcaattcgc
    13981 tccaatgcca ttcgaacagc tgcttgccca attgatttcg cattgatttg aacactggtc
    14041 aagccagggg agaggtttgc gattgtctcg atattatcaa agcttgctac aacaatctct
    14101 tctccaattt ggatattttt ttgtttcaat gcccgataga caccaattgc cattggatcg
    14161 ctcccgacaa gaatggcagt aggaagaggg gattcatttt ctaatagatt agtcgcaagt
    14221 acctcacctt gtttttctgt ccagccacct agatgataag acatatattt ttctaagtga
    14281 tgatcgatca taaaatgctg ataggcacgc atcctctttt catttataga aatctgcttc
    14341 ccaccgaatt catccatatc gactttatat cccccgatat aggctattct tttatgacca
    14401 ttttctaaga acaattccag tacggtttta gtcatccgtt caagatctgc atacaccata
    14461 tcgacctctt gctggatgtc agggttatcc acgacgacta tttgcatgtt ttgttttaac
    14521 aaggcggtaa tcgattcttt cgtaacagtc ccaacaacga tcaccgcacc taactgatct
    14581 agatcttccc attgtttagg attatcagat agattataga ttcgattcat ccctaaatgc
    14641 agacgtcgag cttcttcttc catccctact cgcaaatgtt tgtaatagat atcctgttct
    14701 tcgctcgctt tgccgatcgt agagatcaca ccaatcgaag gcattctctt tttgatatat
    14761 ttacgagtac tgatgtcata atccaacgca gcagctgcct ctactatccg ttgccgagta
    14821 gtctgagcca catggatcgt ctgatcgtta ttcaacacac gggaaactgt agcaggagag
    14881 acatttgcct ttcgtgcgac atctctaatt ccaaccattt ttacacactc ctcaaaaaaa
    14941 taaaaaattt ttattgacaa aattgtttag taaacagtat acaggaatta taaaaaaaag
    15001 taaacggttt ccgttttgtt gggagtgaaa tgatggatat tacgcataga aaaacagata
    15061 aaaatttcat cttatttgat gaaaagagcc gagtcttcca tttacgaaat acatttcttt
    15121 cttatctgat aaagatagaa gaaagcaatg tattggcgca tatttatttt ggcaaaccag
    15181 tcaagcagta caaagacaat aaaaattatc cccgaagaga tcgtggattt tcaggaaatg
    15241 ttcccttaaa tcctgaccgg tctctgtcga aagatacact accgcaagaa tactcttctc
    15301 atggcagcat ggactttcga acgcccgcat cgatcattca gcgcaagaat ggatcagact
    15361 tactggatct gagatatgac agccattata ttacagatgg gaagccagag atcgaaggct
    15421 tgcctcaaac ttatgtgatg gacaagtcag aggcacaaac gttggtgatt tcacttaagg
    15481 atagagaaac agcgatttat ttcgatttgt tctataccat atttactgat agagcagtga
    15541 tcacacgatc tgtaaagata agaaatgaaa ccggagaaac aatcaaacta gaaaaagcgg
    15601 ctagtttcca gctagacttt gcccatacta ggcgatttga tgaagtcatt gctttgccag
    15661 gagcacatgt caatgaaaga cagatttcac ggcaatctgt tttatctgga acaaaagtat
    15721 ttgaaagccg tcgaggaacg agcagtcacc atatgaataa ctttattgct ttagttcatc
    15781 atcatacgac agaaaatact ggagaagcga tcggacttca atttgtttat tccggcaacc
    15841 attctttcga acttgaaaaa gatcagatca atcaattgag agtcgtcggt gggatcaaca
    15901 gccatcgttt ttcgtgggaa ttgaatgcag gccagtcttt tcaaacacct gaaatgatcc
    15961 tttcttactc aagtcaggga ttgaacaaaa tgagtcagat ccaccatgag ttgctacgtg
    16021 aaagaatcgc aagaggtcgt catcaatttg ctgaacggcc gattctggtc aataactggg
    16081 aagccactta ctttgacttc aatagtgaaa aaatcaaggc gatcattgat gaagcaaagg
    16141 agctaggcat cgaaatgttc gtattagatg atggctggtt tggaaaaaga gatgcggaca
    16201 attcttcatt aggagactgg ttcgagtatg aaggcaagtt gacaaatgga ttacgtgaaa
    16261 tagcagatta tgctcatagc aaaggattga aatttggttt atggtttgaa cctgaaatga
    16321 tctcggttga ttctgaactg tatcgaacac atcctgattt tcttatgcag gagccaggta
    16381 gaatgccttc tgcttccaga tcacaacatg tactggattt tacaaggtta gatgttcggc
    16441 agacgattga gaaacaaatg agaaagattt tagatacgat tcctcttgac tacatcaaat
    16501 gggatatgaa ccgttcgctt tcagatgtct attcgattac ccttgaccca cagagacaag
    16561 gagaagtagc gcatcgttat atgctcgggc tttatgaact acttgaacat ttgacaactg
    16621 attatccgga aatcttatgg gaaggctgtt ctggtggagg tggacggttt gatgccgggt
    16681 ttatttatta tatgccgcag tcatggacaa gtgataacac ggatgcagtg gagcgtatga
    16741 aaatccaata tggaacaagc cttgcttatc cgatttcgtc catcacagct catgtatcag
    16801 ctgtaccaaa tcatcagacc gacagatcta cttcgttgaa aacaagagga gaaacagcta
    16861 tgagcgctgt ttttggttat gaactggatt tgacaaagct gtctcctgaa gaagaaaagc
    16921 aagtaaaaga ccaaattata tcctatcaaa cgattcgtcc ggtgatccag tatggccatt
    16981 attatcgttt ggcgagtcct tttgaagaaa atattgccgc atggatgttt gtgtctccta
    17041 aacaagatga agctattgtg tttttaggga ggatactagc ttcagctcaa ccagcgttcc
    17101 atgaagtata tctgatgggg ttagatgatg aggcacttta tcaggaacag acctcgaagc
    17161 ggatattttc gggggccgaa ttgatgacag ttggacttta cttccccgat tttcaaggtg
    17221 atttccaaac agaactgctt catttcaaaa agttatgaga gagaaggaaa aaagtatgaa
    17281 agcaaatata aaatggttag atgatccaca agtttttaga gtgaaccaat caaatgcaca
    17341 tagcgatcat gtttgttatg caacaaggga agaagtagaa accacatcct cccttaaaca
    17401 gtctttaaat ggatcatggc gtttttgtta ttcgccaaat ccttcggtac gaccaaaaac
    17461 attttataaa aaagattttg atgcctgttc ttttgatgaa atcactgttc ctagtcatat
    17521 tgaattagca aattatgaca agatccatta catcaatacg atgtatccgt gggagggaca
    17581 tgtatttcgc cgtccccctt tcacgacaag ccctgagaaa gaggcggaag ggatcttttc
    17641 agctgctaaa gacaatccgg ttggttcgta tttgacgact tttcgattag aagaaggtct
    17701 aaagggaaaa caaatctcga ttcattttgc tggtgtagaa caagcaatgt ttatttggtt
    17761 gaatggtcat tttatcgggt atgccgaaga ttcgttcact ccgtctgaat ttgatttaac
    17821 accttatttg gaagaagaaa atattctagc tgtggaggtt tacaaaagaa gtacagctgc
    17881 attcttggaa gatcaggact ttttccgatt ttttgggatc ttcagagatg tcttcttagt
    17941 tgctcgagga caaaaccatt tagaagatct ttggatcaaa cctcgcttag atgacaaatg
    18001 tcaaaatggc aaacttgaag tagatttgtc ttttgagcaa gtagccaaaa acagtcagct
    18061 gaatgtagcg atactagatc ctgatgggaa ggaaatctat caacagcaat atccgttaaa
    18121 agagcaaatg acgattcgca tcgatggatt tgaaaatgtg gcactgtggg ggcattttca
    18181 gcccaattta tataagcttg aattggagac gatagacgaa gaaaaaagag taacggagta
    18241 tattgactat tccttcggtt ttcgtaaaat cgaaatcaaa gataaaatca tctacttcaa
    18301 tggaaaacgg atgattatca acggggtaaa tcgacatgag tggcatccga aaaaaggacg
    18361 cgccatcact atagaagata tggaaaaaga tttggctatc ataaaaaatg caaagatcaa
    18421 cggcgtacgg acatctcatt accccaacca gatcacttgg tattatttat gcgaccaagc
    18481 aggaatccat gtgatggcgg aaacgaacct cgaatcccat ggctcatggc aaaaaatggg
    18541 cgcatttgag ccttcttaca acgtaccagg tgcagtacct cagtggcagg cggctgttct
    18601 tgatcgagca aaaacgaatt tcgaaacatt aaaaaaccat ccaagtattc ttttttggtc
    18661 tcttggaaat gaatcttatg cggaagaaaa cattcgacaa atgaacgatt acttcaaaaa
    18721 agtagacccc gatcgacttg tccattatga agggaatttt ccaaatccag cattcgaaga
    18781 ttcgatttca gatgtaaaaa gctgcatgta tgcgccacca gaagagatca ttgactacct
    18841 tgaaaataaa gcggaaaaac cgttcatctt atgtgaatat atgcatgaca tggggaattc
    18901 gttaggtgga atggattctt acatgacgtt gcttgatcgt tatgaaatgt accaaggagg
    18961 ctatatatgg gattttaaag atcaggcgat ccaagtgact gatccaatca caggcagaga
    19021 agtgatgcgc tatggcggtg actttgacga taaaccaagt gactatgaat tctcagggaa
    19081 tgggatcgtt tttgcagatg gacaagaaaa acccgccatg caggaggtaa gatattatta
    19141 tgaaaaatac agtaaataaa agtcatatgg atacggaaaa agttgcaatc gtcttcggcg
    19201 actgtacatt aggtgtcaaa tcggggaata cgcattatat tttttcttat acaagaggcg
    19261 gactggaatc gctcaataaa aacggaaaag aatggctata ccgggaaaca cttccagttt
    19321 tttggcgagc aacgaccgat aatgacagag gtagcggatt tggatttaaa tccggtatgt
    19381 ggttaggagc cggattgtat ccaaaagtca aagaaataga agtactggtg gacaataaaa
    19441 agatcgattt gccgattgct ccagtaaata atcaatattc aagtaatgaa tacgctgaaa
    19501 cggttgaaat catttttaca ctggaaatca acacagtacc aaaaacccaa gtgacaattt
    19561 cctaccaaat cgctggagag ggtaaaatgc ttgtcaatat gcattattat ggacaaaaag
    19621 gattaccgga attacccgca ttgggtttaa ggttcattat gccaacgaaa gcaaaatttt
    19681 ttgagtatga aggcctatca ggggaaacat atccggatcg gatggctggt ggtgtcgaag
    19741 gtatttataa agtggcagga ctccccgttt ccccatacct tgttccccaa gaatgcggca
    19801 tgcatatgga aacgaaatgg gtagaactaa caagaaatca aacgttggat aatacggata
    19861 aagatcaaga agatttttcg ttgcgatttg aacagcagga tacgccattt gctttttccg
    19921 ctttaccatt caaagcagaa gaactggaac aggcaacaca tatagaagaa ctaccgccag
    19981 aaagacgaac ggttcttacg atttttgcaa aagtaagagg agtcgggggt atcaatagct
    20041 ggggagcgga tgttgagtca ccttaccgaa tcagcagcga agaggaccat gaaatcggct
    20101 ttattattca gtagcaatct ttagattatt atttgaaaaa cggagtttac aagacaaagc
    20161 tgctttattc tatatgtcat gaacaagaga gtaaaaaagt tctcagatac ttttgttcat
    20221 gactattttc ttcaataaaa ccaattgtta tttttctgaa aaacaaagtt aactatcaga
    20281 aaattaaaaa aag
//
LOCUS       DAZKMK010000049        20197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000049 DAZKMK010000000
VERSION     DAZKMK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.049"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG8V1_001672"
     CDS             291..1430
                     /locus_tag="ACG8V1_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0329767.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG8V1_001673"
     CDS             complement(1497..2072)
                     /locus_tag="ACG8V1_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0329768.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG8V1_001674"
     CDS             complement(2077..3051)
                     /locus_tag="ACG8V1_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0329769.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG8V1_001675"
     CDS             3334..4302
                     /locus_tag="ACG8V1_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0329770.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG8V1_001676"
     CDS             4415..4693
                     /locus_tag="ACG8V1_001676"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0329771.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG8V1_001677"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG8V1_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0329772.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG8V1_001678"
     CDS             5150..5905
                     /locus_tag="ACG8V1_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0329773.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8V1_001679"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8V1_001679"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0329774.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8V1_001680"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8V1_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0329775.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8V1_001681"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8V1_001681"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0329776.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8V1_001682"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8V1_001682"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0329777.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8V1_001683"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8V1_001683"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0329778.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG8V1_001684"
     CDS             10642..11184
                     /locus_tag="ACG8V1_001684"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0329779.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8V1_001685"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8V1_001685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0329780.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG8V1_001686"
     CDS             12777..13679
                     /locus_tag="ACG8V1_001686"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0329781.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8V1_001687"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8V1_001687"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0329782.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG8V1_001688"
     CDS             15122..15544
                     /locus_tag="ACG8V1_001688"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0329783.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG8V1_001689"
     CDS             16013..16243
                     /locus_tag="ACG8V1_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0329784.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG8V1_001690"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG8V1_001690"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0329785.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG8V1_001691"
     CDS             17654..17830
                     /locus_tag="ACG8V1_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0329786.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8V1_001692"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8V1_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0329787.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG8V1_001693"
     CDS             18325..18708
                     /locus_tag="ACG8V1_001693"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0329788.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG8V1_001694"
     CDS             18877..19761
                     /locus_tag="ACG8V1_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0329789.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG8V1_001695"
     CDS             19875..20111
                     /locus_tag="ACG8V1_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0329790.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         6722 a         3525 c         4381 g         5569 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aaccttt
//
LOCUS       DAZKMK010000050        19776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000050 DAZKMK010000000
VERSION     DAZKMK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.050"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            263..580
                     /locus_tag="ACG8V1_001696"
     CDS             263..580
                     /locus_tag="ACG8V1_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0329791.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(784..1149)
                     /locus_tag="ACG8V1_001697"
     CDS             complement(784..1149)
                     /locus_tag="ACG8V1_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329792.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1357..1839
                     /locus_tag="ACG8V1_001698"
     CDS             1357..1839
                     /locus_tag="ACG8V1_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329793.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2036..2926
                     /locus_tag="ACG8V1_001699"
     CDS             2036..2926
                     /locus_tag="ACG8V1_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0329794.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3251..5722
                     /locus_tag="ACG8V1_001700"
     CDS             3251..5722
                     /locus_tag="ACG8V1_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0329795.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5825..7561)
                     /locus_tag="ACG8V1_001701"
     CDS             complement(5825..7561)
                     /locus_tag="ACG8V1_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0329796.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7558..8262)
                     /locus_tag="ACG8V1_001702"
     CDS             complement(7558..8262)
                     /locus_tag="ACG8V1_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0329797.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8393..8896
                     /locus_tag="ACG8V1_001703"
     CDS             8393..8896
                     /locus_tag="ACG8V1_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0329798.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8856..9194
                     /locus_tag="ACG8V1_001704"
     CDS             8856..9194
                     /locus_tag="ACG8V1_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0329799.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9215..10633
                     /gene="ffh"
                     /locus_tag="ACG8V1_001705"
     CDS             9215..10633
                     /gene="ffh"
                     /locus_tag="ACG8V1_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0329800.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10745..11323
                     /locus_tag="ACG8V1_001706"
     CDS             10745..11323
                     /locus_tag="ACG8V1_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0329801.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11327..11848
                     /locus_tag="ACG8V1_001707"
     CDS             11327..11848
                     /locus_tag="ACG8V1_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0329802.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11927..12481)
                     /locus_tag="ACG8V1_001708"
     CDS             complement(11927..12481)
                     /locus_tag="ACG8V1_001708"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0329803.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12734..13009
                     /gene="rpsP"
                     /locus_tag="ACG8V1_001709"
     CDS             12734..13009
                     /gene="rpsP"
                     /locus_tag="ACG8V1_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0329804.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13021..13272
                     /locus_tag="ACG8V1_001710"
     CDS             13021..13272
                     /locus_tag="ACG8V1_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0329805.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13397..14188
                     /locus_tag="ACG8V1_001711"
     CDS             13397..14188
                     /locus_tag="ACG8V1_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329806.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14358..14879
                     /gene="rimM"
                     /locus_tag="ACG8V1_001712"
     CDS             14358..14879
                     /gene="rimM"
                     /locus_tag="ACG8V1_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0329807.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14869..15630
                     /gene="trmD"
                     /locus_tag="ACG8V1_001713"
     CDS             14869..15630
                     /gene="trmD"
                     /locus_tag="ACG8V1_001713"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0329808.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15766..16113
                     /gene="rplS"
                     /locus_tag="ACG8V1_001714"
     CDS             15766..16113
                     /gene="rplS"
                     /locus_tag="ACG8V1_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0329809.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(16245..17381)
                     /locus_tag="ACG8V1_001715"
     CDS             complement(16245..17381)
                     /locus_tag="ACG8V1_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0329810.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(17497..18063)
                     /locus_tag="ACG8V1_001716"
     CDS             complement(17497..18063)
                     /locus_tag="ACG8V1_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0329811.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(18199..18852)
                     /locus_tag="ACG8V1_001717"
     CDS             complement(18199..18852)
                     /locus_tag="ACG8V1_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329812.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18902..19330)
                     /locus_tag="ACG8V1_001718"
     CDS             complement(18902..19330)
                     /locus_tag="ACG8V1_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0329813.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(19335..19709)
                     /locus_tag="ACG8V1_001719"
     CDS             complement(19335..19709)
                     /locus_tag="ACG8V1_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0329814.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
BASE COUNT         6391 a         3530 c         3863 g         5992 t
ORIGIN      
        1 aatcttaaaa ttatcttctt actatgaata agataataaa atttgtattt gtccttttta
       61 ggtaacttat tttaatctat atacctttct ggatcaacga aagtatactt tatatagtca
      121 taacgccgat ggtaactatg cactgtaacg ataatgctgc taaaatatct ttttacgaaa
      181 aacatcacaa ggaaaaactt aactatggta ttatctaaac ataatttttt cggtatttaa
      241 tatttaatta ggagggaata ttttgctttt ttatagtaat tttatcttaa tagtagcgat
      301 attattactt ctgaatatat ggatttttga taggagtaga aatgcaagta taggttttag
      361 aactaaacga agtctgtctt ctaaaaaaaa ttgggtatac tcacaaacta ttttttatgg
      421 aggaattgtt ttaattagtt tactttcttc aacactttat tctttgaata ttatagatgt
      481 ttccacctca aattcaatat ctattattgg aatcattatt gctgcaatta ttacacaact
      541 gtttttagtg tttggagaga agaaaagaag taaaaaatag aaaaactagt gatagttttg
      601 gtgatagtga tattaaaaat cagaaaagat agaaaaagca ggagatagat aaaatgctga
      661 tatttcagca ttttttaaga gtataaaaga gaaaaaaagt aataaatgta tacatgatat
      721 aagaaaaaca tatatgtata aaaagaagaa agtagcttct ctttgtacat atatggtttg
      781 tttttatgga tgccgagctt ttttataaga taaggctacc gcccataacg acccaaggat
      841 ttgtggaact aagtaaatac ctgcaagaag caagatcagc tgggtcggtt ttaaaggatg
      901 atagaagtaa cgccattcag taaaattggc gcaaaataaa taggaagtga cagtagatat
      961 aatattggca atgaataaag atataatacg cctgttcctt tgaaaataat atccagcaaa
     1021 aagagataaa aatatcaatg tgatgaaccc gcttacggaa tgataacgaa aatcaagcag
     1081 taaggcgaaa tgcacataag gaacaaaaga taaaagcaac ggaaggtttt tccagcaagt
     1141 ttttatcatc tttcatcacc acctaacaag tgttattatt ttgttagttt tattgtaaat
     1201 tgaatcctgc aataatcaat tactttttca ttatttctag attaaatgaa taaactctca
     1261 tgccattatc tctaaaaaaa aagtttttcc ccctaatttt tttagataga aaattgtata
     1321 gtatgatgag agaaaaagta gtttggggga acgttaatga gaagaaaagc agtaatcgat
     1381 cggctttatt tatttttttg gttttttcct atcgttttgc taccttttac atgggtaaat
     1441 gctgttgtac aaggtgcgga gatattaggc ctgatccaag gaagcggatt tgtggtttct
     1501 aggaattttc cagctttgac tgttttctat ttagtgataa gtgtaggtct cttattacca
     1561 aaatctctga tcaatcacta caattggcag ctgctttact tttcttgctt attagtattt
     1621 acatctatgt tttctttgct gcctcgcttg attatcggcc ctaatggttt gagcgagagt
     1681 ggattgaagg attcagctta ttcctccatg tatttcttga cgatcaaacc atggttctat
     1741 ttagtaattg cctcatttat tcttgcaaca tttttttact tctggacaga aatcaggata
     1801 aaaagtgaac gatctgtatc tataaaaaat aactattgat ggtggatcat cagtaaaaaa
     1861 gcatatgagc tgcgtaaaat gcatgtttct catatgcttt ttttattatt atgtaaagaa
     1921 tgcgaaaaac gtttacatga tttttacata acttaactat atttttacag caaccatacg
     1981 agggatttac agtcgactga taaactttag ttgtaaataa gaaaggagca ataaaatgaa
     2041 aaaagtattg tttactctag gtatgatggg aattctttta gccggatgcg gctcaggatc
     2101 aggaaataca gatggctctg ccacacaaaa taattcaagt gactcaaatg aacaagtaaa
     2161 aatcgttgcg gtaggttcga cagcattaca gccgttagtc gatgcagcac aagaaagctt
     2221 tgtacaagaa aatccaaatt atcaaatctc tgtacaagga ggaggaagcg gaactggttt
     2281 gagccaagtc gaagctggag cagttactat cggaaactct gatgtatttg cagaagaaag
     2341 agacggagtt gatgcttcaa aattagtgga tcataaagta gcagtggtcg ggatggcgcc
     2401 aatcgtcaat aaagatacag atgtcaaaga tatcaccaaa caggaattga ttgatatctt
     2461 tacaggaaaa atcactaact ggaaggaagt tggcgggaaa gatcaaaaaa tcaacgtcgt
     2521 gaaccgtgca aacggcagcg gtacccgcgc gacattcgaa aaatggggac tggatggtgc
     2581 tacccctgtg cagtcccaag aacaagattc atcaggaaca gttcggcagc tggtaagtca
     2641 aacaccagga gcaatcagct atttagcttt ctcatatctc gatgattcca cacaagcact
     2701 aagtatcgac ggtgtagaac caaaagaaga gaatgtagca gataacagct ggggaatctg
     2761 gtcatatgaa catatgtaca cgaacggaaa accttcccct gaagtccaaa aattcttaga
     2821 ctacatgatg acagaagaaa tccaagaagg acctgtcaaa gaattgggct atttaccaat
     2881 cacaatgatg gaagtagaac gtgatcacga aggaaatatc aaataactag cagataaaca
     2941 tgccacggta gtcgttacta ttaaggaaaa atcaactatg tttcccacat atacattttt
     3001 cctttaattg tgaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
     3061 cagctgatta aacaccgttt attcgaaaaa aagagctacg acatagaact gtcgtagctc
     3121 tttttttgct atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc
     3181 catatctgtt ctatactgga taggagaagt ttggaaatat ccagaaaacg aaatcaatcg
     3241 aggtagccac atgaagaata aatggataaa tcgaagtaaa aaaaagaaag taacacaaag
     3301 atcatcagat aaagtcatcc gtctttttcc atcgtataaa gaaggattaa cggcagatca
     3361 agtcaaggaa cgaatagaaa aaggtgcagc aaataattca gtcgatccca cattcaaaac
     3421 aaatcagcaa atcgtattgg aaaacatatt tacgtatttc aatttgatct ttcttatctt
     3481 agccattttg ctttgtttag tagaatccta taagaacttg acttttttac cagttatcat
     3541 agccaataca ggaatcgcca tttttcagga aattcgatcc aaaaagatct tggacgaact
     3601 aaatgtttta aatacagcaa aagtcaatgt catacgggac gggatagaac aaacaataga
     3661 tatagaagac cttgtaatag atgacattgt tattttagag actggacatc aaattccagc
     3721 agatgcagag gtcgtggaag gaaaactgca agttaatgag gctttattga caggcgaagc
     3781 agatgaaata accaaagaaa taggcgatcc attaatgtcc ggcagtttca ttgtttccgg
     3841 aaaagcttac gcccgattag acaaagttgg agcagattcg tatatttccc agctgacagt
     3901 caaagccaag acaatgggag aaggagaaca gtcggagatg attgcttctc ttaatcaatt
     3961 gatcaaatgg attgggatca tcattattcc tatcggtatc ctgctttttt ctcaaagtta
     4021 cttttttaat ggaaacacga tcaaagaaag tgtcgttgca atggaagcag cattgatcgg
     4081 tatgatacct gaaggactgt acttgctgac gacgattgca ttagcattaa gtgcgactaa
     4141 actagcaaag caaagagttc ttttacacaa tatgaaaagc attgagacat tggcaagggt
     4201 aaatgtcctt tgtgtagata aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa
     4261 agtgatcgtc tctaaaaaac aagaaataat cagtcagtca gatcaattgg aagcatgcat
     4321 tgctgactat gcaaaagcga tggcaggaga caatgcaaca atggaagctg ttaaagcata
     4381 ttttacaaaa accactgatc aatcctatca ccatgttatt cctttttctt ctgtccaaaa
     4441 attcagcagt gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg aaatggtttt
     4501 gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg
     4561 cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa ccagtattga cagaagcagt
     4621 cacaccatta ggctatattc tgattgctaa tcctatcaga aaagaagcaa gagccacatt
     4681 tgattacttc aaaaaacagc aagtagccat caaagtgatt tctggagata atccgctcac
     4741 cgtatccaat gtagctgtcc aagcagggat ttcacatgct gatcagtacg ttgatgcatc
     4801 taaattatca gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt
     4861 caagccagaa caaaagaaaa tcttcgttca attgataaag cgtaacaata cagtagctat
     4921 gacaggtgat ggcgtgaatg atattctagc catgaaagaa gcagactgca gtatagcgat
     4981 ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg attctgactt
     5041 ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc
     5101 agccagcttg tttttagtga agaatatttt ctcctttttg ctctcactgt tttcagtgat
     5161 ctttgctctg acctatcctt tagaaccatc acaaatcaca ttgattagtt tgtttactat
     5221 cggacttcca tctttcttgc ttgctctaga agaaaacaaa aagcgaatca gagggaaatt
     5281 catcatgaat gtgatggaaa aagctgtgcc aggtggacta acagatatga tcgtagtcgg
     5341 tgcgttggtg atctgcgggg tgactttgaa tctaaataaa acagatgttt ctacagcatc
     5401 taccatgctt ttgattgcag tagggttcct cgttctctat aaaatctgtt caccgctgaa
     5461 taaattcaga agtcagatca ttcttttctg tgctagcggt aacttttttt cggtcatctt
     5521 tttgcataag ctgttttcaa tcaccggtat ctcagctgta tcacttttgc tgttgagcat
     5581 tctatttttc tcggcagatt cgatttttag gcatttgacc acattggtag aatatttgtt
     5641 tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc
     5701 gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca
     5761 ctcgtgtatc tttcgaagca ggatgagtca tccaacttta aaacgcacga gcagttgctc
     5821 ttttttacat tttcggaatg gtaacggtga aagtcgaacc tgttccaaga tgactgtcta
     5881 cagtaatcgt accattaagg agttgcacgt agtcttttac aatcgctaac cctaatccgg
     5941 tcccgcctga atgtcggctt cgcgccttgt ctacgcggta aaaacgctca aagatccgtt
     6001 cctgatcctc aatgtcgata cctataccaa agtcttggac agaaaacgat agatcatctg
     6061 ttgcctgata gcggataata atctcgctgt cgctttttga atattggaca gcattttcaa
     6121 tcagattttt gataattgga taaaggatat ctgttttagt agtaaatggt tcgttctttc
     6181 caatcaatcg aatcgtcaac cgtttctttt cgatgattgg ctgataattt tgaacgattt
     6241 gctggaaata aggttctgta taaattgtct ggatctcgta agaattctcg ccatcttttg
     6301 acagctggat gatttcccga atcagtttat ctaaccggat cgcatctttt tgcatgatct
     6361 caagaaaaga agtcagagtt tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa
     6421 aaccaatcag tgaagtcaca ggagttttta attcgtgaga gacgttgcca acgaaatcct
     6481 tttgtagctt ttctaaacgt cgcactttcg ttaagtcata cgccacccct agaatctgtc
     6541 cggtaccttc tgaattagta aagtagcgca aactcaagtc taatgtctgc cgaccttctg
     6601 taagggtgat ctcctgatgg atcagcggat gctcaggagt cacttgatga atcagctgaa
     6661 tgagttttgg ctcttgaatg acttctgtgt accgctgttc aggttgatag cttaccacgt
     6721 ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg agaatcagca tcatcaatca
     6781 ggaatacgcc aatcatcagt tcgttcagta acgtatataa ttgctcttct gtggtagtac
     6841 acgcccgata catcttactc atttgttcgc tcaacgcatt aatcgtctga tacaattctt
     6901 cccattgatc gggtgtttgc atgattactt ccgcctgatt aggattttgc accatttttt
     6961 ttagtacggg cagcacggtt ttcaatggct ggtttttttg atagatcaga taatagatca
     7021 ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa gacccaacgg cgaaaactct
     7081 cagttctcgg taaaaaaccg cttgtcggtt ctgcgatacg taaaatggct tctaactggc
     7141 cacttttttt gactggtaat gcgacatata aaagatcctc attcaaagta gtacttttgc
     7201 gcaaagcgct acctaggttc ccaccattca atacggcttt gatttcggga cgggagtttc
     7261 gctgttcatt caatgtttca ttgtttgtgt caaataagat ctttccagta gcatccatta
     7321 aagtaatccg ttcgttcgac tgatggacga attcttccaa agcagttttg ttttgcgctg
     7381 cttggaggtt ttcgacatct aattgccgga tcagcagttg tgcttttttt gtcaggtagc
     7441 tttcttgctg ttccacgatc tgctgttgaa aataatggga aatcaattgc cagccaccaa
     7501 aaaacaaact aactaaaaga ataagaaaga agagacgttc ataaattttt ttccatttca
     7561 tcgttttggt tcctgaaact tatagccaaa tcctctaata gtcaccaagt actttggatg
     7621 ttttggatct cgctcgattt tttctcgcaa atgactaacg tgtacatcta cgattcggct
     7681 ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg
     7741 gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag
     7801 atggtcgtct acggtcactt gataattggt caaatcagca gtgatttgtc caatcgatag
     7861 atattcaggc tcagcttcat cttttgattg cttacgaggt tcaatccgtc tgaatattgc
     7921 tttcatacgg gctaatactt cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc
     7981 tatttctagg ccaatgatcc gatccacttg gtcatcttta gccgtcagca tcaaaatcgg
     8041 tgtttcattt ttttcttgac gcagccgctg agtgatctcc atgccatcca tcgttggaag
     8101 catcacatct aaaatgatga aatcaaatga ttgttctaat gctaattcta atccttcgcc
     8161 tccatctgtg gcactggtta ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt
     8221 tacaatagaa ggttcatcat caacaactag tatttttttc attcaaatct cctttaaata
     8281 ggtttcattc tgtttatatt ttagcataga atcagtacaa agggctattt tacaaaagaa
     8341 ataaatggta taataggaag gcactttgct gaatagaaag gaagagcagt caatgattgg
     8401 aatcagtgca tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa aagaagtacc
     8461 agaattgatg gaactagttg aaactggaga agctgtcatg gtctgtccag aagtgatcgg
     8521 cggattacca attcctagag atcccgctga aatcattggt ggaaatggat tcgatgtgtg
     8581 gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa
     8641 tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac attgatctag tcatcatgaa
     8701 ggaaaaaagc ccttcatgcg gaacccattc gatttatgac ggaagttttt ccggaataaa
     8761 aaaaaatggt gccggagtgg ctactgctta ttttattcag caaaaaatga ctgtgctctc
     8821 tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag accaatcgaa
     8881 tgaacgcgct ctttgaattt tattcgacct tattgacaga aaaacaaatg aactacatgg
     8941 aattatatta tgcagatgat ttttcattag gtgagatcgc ggaagaatac gaagtaagcc
     9001 gacaagcagt atatgacaat atcaagcgga caagcaagat cttggaggat tatgaaaaaa
     9061 aactccatct tttttctaat tatattgttc gagaacaggt attggaaaat atgactgcat
     9121 atattacaga taagtacccg gaagataaaa aactacttga atatgtacag caaattcaag
     9181 caatcgaaga ataaataaaa ggaatgaaga aaatatggca tttgaaagct taactgaacg
     9241 tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa
     9301 agaaatgatg cgggaaatcc gccttgcctt actagaagcc gatgttaact tgcaagtagt
     9361 caaagatttt actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt
     9421 atcacctgca caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca cacttggttc
     9481 tgaaacagca gagttaaata aatcaccaaa aataccaact gttatcatga tggctggtct
     9541 tcaaggggca ggtaaaacga cctttactgg taaattagcc aattatttaa agaaaaatga
     9601 aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga ccagctgcga tcgaccaatt
     9661 gaaagtatta ggtcaacagc tagacgttcc tgtatttgat atgggaactg aagtcagccc
     9721 ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg actatgtatt
     9781 gatcgatacg gcaggacgtc tgcatatcga tgaaacattg atggatgagt tgaaacaaat
     9841 caaagaattg acgcaaccaa atgagatttt gcttgttgtc gatgccatga ctggtcaaga
     9901 tgccgtcaac gtagctgata gcttcaacca gcaattaggt attacagggg tcgtcatcac
     9961 taaacttgat ggggatactc gaggcggggc agcactatcg atccgctcag tgacaggcgc
    10021 accaatcaaa ttcatcggtt ctggtgaaaa attgactgac ttagaagtat tccacccaga
    10081 tcgtatggct agccgtatcc ttggtatggg ggatatgctg acattgattg aaaaagcgca
    10141 gcaagactac gacgagaaaa aagcggaaga acttgcacag aaaatgcggg aaaattcatt
    10201 cgatttcaat gactttattg aacagcttga tcaagtgatg ggtatgggcc cactggaaga
    10261 tctaatcaaa atgatcccag gaatgaatca agtccctgga atcgaaaacg tcaaggtaga
    10321 tccaaaagat gttgaacgca aaaaagcgat ggtttattct atgacgccag cagaaagagc
    10381 aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt ctggaaacag
    10441 tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca
    10501 aatgtccaaa ggggatatga acatcccagg tatggaccaa atgtttggtt caggcgtcaa
    10561 aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa
    10621 aaaacggaaa taagatgaaa aaaacagctt cggtacagac tgaaaatgtc tactgccgga
    10681 gcttttctat cagcaatttt tattctttac taatacaatt actctaaacg aagaggtggc
    10741 acagatgaga tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc atgatactgt
    10801 ctggggtgtt cctgaatatg atgaccaaaa gctgttccga aaattgatgc ttgatattaa
    10861 tcaagccgga ttgagctggc agacgatttt aaataaaagt gaagcattcg atgaagcata
    10921 tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat
    10981 ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa
    11041 agcagtcttg aaaattcaag aagaaatcgg cagtttttca gagtatcttt ggtcgttttc
    11101 taatggaaaa atcgttgatt cttcttatca ttcacaagaa gaagtcccaa caacaagtcc
    11161 tctttcagat gaattagcaa aagatctaaa gaaaagagga tttaaattta tcggaagcac
    11221 aacaatctat gcttttttag aagctgtcgg gatcatcaat gatcaccttg acagttgttt
    11281 ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat
    11341 attttatggt gctataagtt tagatggcta tttggcaacg aaaacggatg atcttcaatg
    11401 gctttttgac actccaacag gtaagcaaac tacttatgaa gctttttatg aaacaatcga
    11461 tacgaccatc atgggcagaa aaacttatca ggaagcaaaa aaatatttgg agaccgaagc
    11521 aatttatcca gagaaaaaga atttcgtttt ttccacccac gcacaattac aattgccaga
    11581 tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa tgacagaagg
    11641 aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg aacatcaaat
    11701 gattgatgaa tggtatatcc agattacccc tgtattatta ggggacggga ttcctttatt
    11761 ccaaaaagga acatatgaag cgcgttttga aatgctagac acgacacgat tcggtgaatt
    11821 tatcgaattg cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc
    11881 agaaatgtcc tgtacatagt tgcctttttt attaccgtta tgctttttac tctgttaatt
    11941 tgttagaaaa atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa
    12001 cgatattacc attttcatca attagattcg caacgtttcc gatatatgct tccggttgct
    12061 gcaaggttgg cacattcaaa aagacaagtg attggcgtag atggtggttt gcgccaaagc
    12121 cgccgatccc acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt
    12181 gtccataagg tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt
    12241 tgtattcagg agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag
    12301 tccattcagc aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat
    12361 cgatttttac aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta
    12421 cttttttgtt gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca
    12481 tacgattttt cctccttcaa actatctata ataatcttac aaaagataag taactggagc
    12541 aaataaaaac acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa
    12601 aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa
    12661 aaaagcttta cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag
    12721 gtggaaataa ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc
    12781 gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac
    12841 tgtaggtact tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt
    12901 tcttgactgg ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa
    12961 agaaggcgtt atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat
    13021 atgaaagatt taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat
    13081 caagtcaagt tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct
    13141 cctgaagata ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca
    13201 atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat
    13261 aaggaaaagt aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt
    13321 tattaattga agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg
    13381 agggagtgaa cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag
    13441 cgcttgtagt tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga
    13501 aactgttacc agtccagaaa aaagtagtac caaaacggct tccaccactg aacaaacaat
    13561 ggctacctca aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa
    13621 tgttcccatg cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca
    13681 tacaaagcaa ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc
    13741 tgacgaattg aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc
    13801 tagtcaagaa acaatccaag ttttgcagcc atttgagatc gacacaaatg gtcagcaagt
    13861 ggacttaggt tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga
    13921 atggcaagaa ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc
    13981 cttactatta gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga
    14041 acaatttggt aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag
    14101 ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa
    14161 gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa
    14221 ctccaagtca caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta
    14281 ataccttaaa agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa
    14341 agaaatgggg atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag
    14401 gtatcaaagg cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa
    14461 aaggaacggt gctaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa
    14521 gtcatcgcaa acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg
    14581 atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag
    14641 ctgaaaatga attctattac catgagatca tcggtctgca tgtattagaa gatggaactg
    14701 agttaggtaa agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac
    14761 gtccgaaaaa agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt
    14821 tagaagctgg agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat
    14881 gtattgactt tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag
    14941 gctgtagata aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac
    15001 aaacatcaga cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc
    15061 cagcccatct atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga
    15121 gtaattcttc ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca
    15181 caagaagaac acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc
    15241 acattagtga cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt
    15301 gcgatggtca tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact
    15361 tcagctcaaa cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg
    15421 gcagaatata aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt
    15481 gaagaatggc aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg
    15541 gaaacacttg aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa
    15601 caagaaaaaa cagcagatga agccaagtga ctttttctaa aatcttcttt acagtaggtt
    15661 gataatatgt tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct
    15721 gtgagaaaag ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc
    15781 gaagaattaa caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact
    15841 gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt
    15901 gttgttatca aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct
    15961 aacggagtgg gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa
    16021 gtagttcgct acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga
    16081 aaagctgctc gtatcaaaga aatccgtcgt taatcatcgt tatcagacag agaaagaacc
    16141 cttggtattc aagggttctt tttttattat ctgttttttt atattaacga tttctatttg
    16201 gtacattttt gattaataaa aaggggcaaa aaaggggcaa caagttacag tttttctata
    16261 tcttctttca atttttcttt tactcctttt gtcacatgca aataaatttt ttcagtgata
    16321 tcactatttt cgtgaccaac gcgatcctga atggcataca gaggagttcc tagttccgct
    16381 aattttgaaa tatgggtatg tctaaaaata tgtgaactaa gttttttatc aatcttcatg
    16441 tcagctttat gacttctcaa ataggtgtta attgctgtta gttggaaagg agttcctttc
    16501 gttgtttgaa atagaaattg accatttgga tttaattcta gaagctcatt gtaaatagct
    16561 atcgcttttt ttggtaaatc aatttctcgc attccggcag ctgtttttgt agaatcagat
    16621 tttttcatat cagctattga acgttctcta tacatcatcg tcccatttat aactactgat
    16681 gcattattat tggtaatgtg tacatcatct ttacttaatg cgattgcttc accagacctc
    16741 atcccagtca aatatagcca ctggaaaagg agagaatatc ttttattgtg tgaagttgtg
    16801 aaatctacta gtctattata ttcatcatct tctaaaaatt tatctttaat ttttatcgtt
    16861 tttgactctc ttttataatc aataactact tcatcgatag gatttttttc tacataacct
    16921 tttttcatag catatgaaaa aagaagattc aatttggatt taattacact gacgtactta
    16981 tttgacaagt catctttata tatcatgtct tcaaaaatat tattcaaatc tattgtatta
    17041 ataccagaaa cgatgtaagt ctcaggtatc ttcttcttta tagtgtttaa tatattattc
    17101 gtaggataat atgtagattc cttaacttgt cttttgtata taactaacca ttcttcaaca
    17161 agctcatgaa atgttagatc aggcttatga agtgtttttt cgtttaattt tatatcaatt
    17221 tttttattta actctagcaa tgccactttc tgagtttctc gagatttatt tttataagta
    17281 atgctcactt tttttctttt tctagttttt ggatcaatat atcgctcatt atatttatat
    17341 actttttttc cgtttttatc agttttagtt tcgatccaca ttgttttcat ctcctatttg
    17401 ttgctataat aggaatagat aagtaagccg attatagcag gtttattttt catcacgtcc
    17461 acaaactttg gcgagggagg ggcgtgtttt tgttagttat tgtggtaggt tatctaaagc
    17521 gtattgtgct tgctcatttg taaaaccttc aaatataagt tgatcgtaca attgcgaatc
    17581 tgacattgat gcccaatcat cgtaactttc cgctttagcc aaagcttgtt cgttgtaatc
    17641 gacatcaaca ttagccagcg cataatcgat tgcatcactt ggatattctt caaactctag
    17701 ttgttcacgc aaaccttgtt cagacatacc agcccaacct agatagcttt cagctttgct
    17761 caatgcattt ctgtattcac gaggaacact ttcttcttga gtaacttctt ctatagtagt
    17821 ttcagtagtt tcagtttctt cttcaacgac tgtagattct gctaccgatt ctgcagaaga
    17881 cacttctgtc acgctttctt tagttgtttc agcgacggta tttttagcat catcactgcc
    17941 tccttgcgaa cctatagcaa taaacaccac aacaaccgct aacaaccaaa accatacccg
    18001 tttataaaac ggtttcttaa ctttatacat tttcccatct tgacccataa ctttttttgc
    18061 catttaaata tccctcgctt cttgttataa tatatttgtg atctcagaaa tgaggtatga
    18121 gtccgtgttg cagcacgggc ttttttcttt ataacttttt tagagattat aggcaaaata
    18181 gtagggcata aaaatgtatt attgaattcc gtatttagaa aatcctaatt gaacttcacc
    18241 ggaagtcttt tgctgtgttg tacgcaatgc ttcttcttca gacattccat tctgtacttt
    18301 ccatgcaaca ggcgacatcc cgtatttgtt aacaaaatca gtaagtgata aagtgtcagc
    18361 gtcttgctga gcgcttgttt gttggtcttc tggattctgt tgagatgctg cttgttgttc
    18421 tttctgatct tgactgataa tattgccagc atcatctgta gtcaatccat tttcataaag
    18481 ggccacgccg aaagcttccc actctttgtt ggaccaattt gcacgatcag ctggagttga
    18541 ctgtaaagtg cgttgtttca tctgttcata tgtttcttct tgaggtgcgg tttggattgt
    18601 actctgactg gagcttataa ctgttgggct aggttccgcc gtagcttggt tggagcttgt
    18661 aactgttgaa ctagaatctg ttttagatgt agatttgcta gtagatgaac tggtttcagt
    18721 tgtttctttt gttttactta tttttgtttc ttggttagaa gtggtatctg ttgattcagc
    18781 ttttttatta tttgaacaag ctgaaagtag cagagcagta cttaacaaca acataacgct
    18841 aacttttttc attttataat tcctctttct cgttgtaata tgtgtgctaa cacgggcttt
    18901 tttatataag aaaacgataa gcgctttctg gaagcccgta aagattcttt aattcctcga
    18961 tttttttagg atattgatca ttatcttctt tataaagaga aacaatgaga ttagcagcaa
    19021 agcaattagc ttcgctttca gatttgcttc tagatgttct tgttgataca taatagctgg
    19081 ataagccacg atgaaaaata gcgtgaccta attcgtgagc gcaaatgtag aatctttcct
    19141 cagagtctcg cagttcatca tttaaaaaga ttatcgcacg atctctaatt tcttgaaact
    19201 gccccttggg attctcgata aaaggaacgt attgaatttt aatgcccatc ttttcacaaa
    19261 tataaaaagg attagcggac tggtatttcc gcttcaactc ctcgactaaa ttaatcgtat
    19321 ccatctccat aagctcacat ctttttgcct ttttctttgt cttctttcac aatatcccag
    19381 aaagtcgcta tcaggatatc ttttacgcgc tgtatttgtt cgggtgtcaa tgtttcccca
    19441 ccataagaca tattaacatt tgagtctagt agtttatcaa gttcaaccac ttcctctttt
    19501 gtagcccact tgggaacatt attatttccc aataaataat cagttgtgac accaaaataa
    19561 tcagcaactt tctttaagtt ctcagatttt ggcgaggctt tatcccatct tcttatttgt
    19621 ccattagaga tgcccacctg tctttctact tctgctatag tcacatgctt ttcgtctgct
    19681 aattctttaa tcttagtaac taaactcatt attatcaacc tttcaaagct gaaaagaaaa
    19741 taaatagctt aaaagttata tttttggttg acaatt
//
LOCUS       DAZKMK010000051        19514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000051 DAZKMK010000000
VERSION     DAZKMK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.051"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8V1_001720"
     CDS             133..3660
                     /locus_tag="ACG8V1_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0329815.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8V1_001721"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8V1_001721"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0329816.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8V1_001722"
     CDS             7448..7789
                     /locus_tag="ACG8V1_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0329817.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8V1_001723"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8V1_001723"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0329818.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8V1_001724"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8V1_001724"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0329819.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8V1_001725"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8V1_001725"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0329820.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8V1_001726"
     CDS             13000..13737
                     /locus_tag="ACG8V1_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329821.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8V1_001727"
     CDS             13751..14566
                     /locus_tag="ACG8V1_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0329822.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8V1_001728"
     CDS             14579..15226
                     /locus_tag="ACG8V1_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0329823.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8V1_001729"
     CDS             15242..15898
                     /locus_tag="ACG8V1_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0329824.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8V1_001730"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8V1_001730"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0329825.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8V1_001731"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8V1_001731"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0329826.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8V1_001732"
     CDS             18240..18758
                     /locus_tag="ACG8V1_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0329827.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8V1_001733"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8V1_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0329828.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6563 a         3348 c         4187 g         5416 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtga
//
LOCUS       DAZKMK010000052        19366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000052 DAZKMK010000000
VERSION     DAZKMK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.052"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(398..1351)
                     /locus_tag="ACG8V1_001734"
     CDS             complement(398..1351)
                     /locus_tag="ACG8V1_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0329829.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1374..2129)
                     /locus_tag="ACG8V1_001735"
     CDS             complement(1374..2129)
                     /locus_tag="ACG8V1_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329830.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2126..3085)
                     /locus_tag="ACG8V1_001736"
     CDS             complement(2126..3085)
                     /locus_tag="ACG8V1_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0329831.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3078..4028)
                     /locus_tag="ACG8V1_001737"
     CDS             complement(3078..4028)
                     /locus_tag="ACG8V1_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0329832.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4178..4630
                     /locus_tag="ACG8V1_001738"
     CDS             4178..4630
                     /locus_tag="ACG8V1_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0329833.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4719..6986)
                     /gene="gshAB"
                     /locus_tag="ACG8V1_001739"
     CDS             complement(4719..6986)
                     /gene="gshAB"
                     /locus_tag="ACG8V1_001739"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0329834.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7173..8171)
                     /gene="ftsY"
                     /locus_tag="ACG8V1_001740"
     CDS             complement(7173..8171)
                     /gene="ftsY"
                     /locus_tag="ACG8V1_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0329835.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8182..8991)
                     /locus_tag="ACG8V1_001741"
     CDS             complement(8182..8991)
                     /locus_tag="ACG8V1_001741"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0329836.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9000..12581)
                     /gene="smc"
                     /locus_tag="ACG8V1_001742"
     CDS             complement(9000..12581)
                     /gene="smc"
                     /locus_tag="ACG8V1_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0329837.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12601..13287)
                     /gene="rnc"
                     /locus_tag="ACG8V1_001743"
     CDS             complement(12601..13287)
                     /gene="rnc"
                     /locus_tag="ACG8V1_001743"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0329838.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13635..15416)
                     /locus_tag="ACG8V1_001744"
     CDS             complement(13635..15416)
                     /locus_tag="ACG8V1_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0329839.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15442..16356)
                     /locus_tag="ACG8V1_001745"
     CDS             complement(15442..16356)
                     /locus_tag="ACG8V1_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0329840.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16383..17345)
                     /gene="opp4B"
                     /locus_tag="ACG8V1_001746"
     CDS             complement(16383..17345)
                     /gene="opp4B"
                     /locus_tag="ACG8V1_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0329841.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17345..18289)
                     /locus_tag="ACG8V1_001747"
     CDS             complement(17345..18289)
                     /locus_tag="ACG8V1_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329842.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18290..19294)
                     /locus_tag="ACG8V1_001748"
     CDS             complement(18290..19294)
                     /locus_tag="ACG8V1_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329843.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5331 a         4196 c         3431 g         6408 t
ORIGIN      
        1 ctcatatttt ctagtctagt ggacttattg ccgagaagca gcagatggaa catcgtttaa
       61 agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg
      121 tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt
      181 tttgccactc cccattctag aatcaacccg tatgatgtag ataatgaatt cacgttgaaa
      241 aaagatttct caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg
      301 tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg
      361 ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat
      421 cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt
      481 caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag
      541 aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga
      601 tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt
      661 cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac
      721 cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag
      781 tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt
      841 tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt
      901 cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt
      961 tttgaaaatc tctttgtcgg ccagaaatga tgattagatc tggctgaagc tgattaattt
     1021 tttctaagtc tggttctttt attccacctg ctgattctac ttttttatag gatgaaagat
     1081 actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca
     1141 tcgtatctaa agaaccattg tcgaatacga cgactttttc tggattattt ggcacctcga
     1201 tttggtctcc attactatct gttatcgtca atgtagtttc agtttgtgtg ctgctttttt
     1261 ttgtgtctgc ctgactggtg ttgttgctat ttccacatgc tgctaaacca aatgatgcta
     1321 gaatcaagat acctactact aattttttca tctcttctct cctaatgtta gttttagaaa
     1381 taaagacaga atctgcggcc ttcaatctca caaatccgta tgtccatttc gtataattca
     1441 tccaatacat ctttttttat catttcttgg gtcgttcctt gcgtaaataa ctgtccatct
     1501 ttcattgcaa caatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata
     1561 acaattgttt tcccgatttc ttgtactaaa ttttttaaaa tctgcatgat ttgaaccgca
     1621 aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct
     1681 agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga
     1741 tccgctattt cttctaaccc aatgtgttcc agtgattcct gaacgatttc ctcgtctttt
     1801 gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg
     1861 atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg
     1921 gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc
     1981 aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca
     2041 gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac
     2101 agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg
     2161 tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga
     2221 aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc
     2281 cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa
     2341 taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga
     2401 cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt
     2461 catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc
     2521 cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa
     2581 ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac
     2641 tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg
     2701 ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa
     2761 cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga
     2821 agaaaaggac cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca
     2881 aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga
     2941 caaggataaa tgcaaaaatt ttcttactcc tcaatgcaca tgcaaaatgc cagtttccat
     3001 atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa
     3061 aaatagatgg ctttttttta agcatatcgt cgtcccctta tcagaagata aaggaagata
     3121 gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg
     3181 cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt
     3241 gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata
     3301 aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg
     3361 cctaaaaact gcatcttctg gtaatctatt cccagattta ctgcaaacga ttcgcccaga
     3421 ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca
     3481 tacaataact cataatttcc tcgtatcaca gtcgcaaaat tcccttgaag ccatgaagtc
     3541 atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc
     3601 cctaacatga ctccaatcaa cggaagcatc aattgatttt tctggaggaa caatcgcgta
     3661 agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca
     3721 atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc
     3781 atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac
     3841 ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc
     3901 actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc
     3961 gatactccga tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg
     4021 tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc
     4081 gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat
     4141 cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag
     4201 ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata
     4261 tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc
     4321 aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt
     4381 gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa
     4441 tagccgtttc actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct
     4501 cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga
     4561 agaaaactct tcgccagctg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg
     4621 tttattttaa aacgaaaaaa cacataaatt tttacatgca aaaaaagctt ttgatcagat
     4681 tgacatcaaa taggacaatc tgtcaaaagc tttttctttt aaaatacttc aggatataac
     4741 aatttcaata cattcatcgt caatcttctg ccttttcctg caaaaggata aacatgcata
     4801 tgcatggctg gattgaaatt tgcttcgatg atcccatatg cttttttatc agttgtagga
     4861 tcgatttctt tatctggtat aatcaaatcg ataccactaa tcttagctcc tagtgcttct
     4921 accgcagaaa cggcgatttt tttgtaagct tcagaaaatt catcagtcat atcgatcgaa
     4981 tcaccacctg tactgatatt tgagttctcc cttagataaa caatttgatt ggcttgggga
     5041 actgattcga tcgtcaaccc ttgttctttc aacatcaatt gttctaattc acctaattgg
     5101 atcaattcta aaggtgcacg atgattcgtt ccacgcaaag ggtcactatt tttttcttca
     5161 acaagttctt tcactgttcg gatactgtca ccaataacat tagcagggac tcgcaacatg
     5221 atggcttgga cttctccgtc gatcacaaag aaacgatatt ccgttcccgg cataaattct
     5281 tctactaaaa ccgaactgtc ttcacgaaaa gcaatggcta aacctgcttg ataatcttcc
     5341 agactagctc cttccttgaa aatcgtgata cctaacccgt aattggttga tttaggtttg
     5401 atcacaaatg cctgctcggc aaatctcgga taagctttta ccgcttcttc catggatgaa
     5461 aattctgcgc ctcctggtac ccggaaccca gcttctttta agactttttt cgttactgtt
     5521 ttgttttcca tgataagtgg gacgatatag ctgtccttac ttgtcatgtt tgcatttttc
     5581 acatattcta catggtcttg gaattgcaga cgcaaaaatt gatccgactc atctaatact
     5641 ttgacttgga cacctttttg gatggcatcg aacataaata tctgagtaga aagttccatt
     5701 tcgcgaaaac cggccaactg atagggacgt tcatggcttt ctttataata ttgatgtccc
     5761 aatgccacag caacttcttg ctgactcgat agctgtgctt cttgatagag tttcgctgct
     5821 aacgtttgtt caggatgatc catttgagtc aatgcttcct ctactaaata aagagaatcc
     5881 aaccaatcca attcaaccag catttgcttc attccttcaa gaatttctct accttcttga
     5941 tgaaattctg tttgatctga tggttgttct agtgctactt tgttatttcg agtcgtacct
     6001 tctgctaccc attggtcaca gtcttctttt tcttctgtcc ataacatgta catcaggaat
     6061 aattgtaaga accgaacttg ctctggattg atacccaatc ttgcataagg attcaaatcg
     6121 atattccgca attcaatata acgaacacct gttgttgcta aatctgcgac tttctttccg
     6181 cctcggaaac gaagcggtgt ataaaattct ttctcctccg aaagtttccc ttcttcgatc
     6241 attcgctcga tatctgctaa atactgtttc atagaagcat aggaaacttt cacatttgga
     6301 tgattcgtat agcccagcgc actgttgcga atactgcgaa caggttcttg cggatgtgat
     6361 tcatctaaaa agtagttctt ctcactcatt ggtgaagcac cgaataaata agtgatcatc
     6421 cagcgatagc gcataaagtt tctcgctgtt ttcaaataaa gttctgtttt gaattcggaa
     6481 aaatcttgaa attcttcctg atggctgaac aatgttctta gcaactcatc gccaaattca
     6541 aaattgaaat gaataccgct taccatctgt tttcgcttcc cgtattcttt tgctaaataa
     6601 cgtcgataca agacatcttc gaaatttttt aattttgcaa taatgatctc ttcgtctttt
     6661 tccggtaagg caggtggcat acttaatggc cagatcatct cttctttcgg tatagaacga
     6721 gcagtcacat cataaacagc agcgagatac tgaaataatt cgggaataga atcggtaaca
     6781 ggggtgatca tctctgtttg tgtttcgcta aaatctgttt ggatataggg gtgataggat
     6841 cgatcgccaa agattgctgg atgatcggtt tttgctagat ttcctgcaag atcaacccgc
     6901 tgtccttccc gttctatacc aaatcgagct tggtcgataa aagggcgcgc attgacatgc
     6961 aataataatt gcttaaaatt catcattaag tgacctccaa ttcatatgaa tctaattata
     7021 atcaaaaagt ctaagaaaaa gaacgaaatt gtttgagaac cgcttacaaa taagtataaa
     7081 aatgaaggca tcaaaaacac agctatgtta aaaacaatca ttcaaacaag gagctgcaac
     7141 ttttgtcaca gctcctgctt tttagacatg ggttattctt cttttagtaa tcctttgaac
     7201 aatccaacga caaaatcatt tggatcaaac ggctccaagt catcgattcc ttcaccaaga
     7261 ccgaccaatt tcactggtaa atgcaattca ttacggatag caagtacgat accgcccttt
     7321 gctgtaccat ctaattttgt caaaacaaga ccggtcacat ctgttgtttc tttgaattgt
     7381 tttgcttgaa ccatcgcgtt ttgaccagtg gttgcatcta atacaagaag tgtttcttgg
     7441 ggtgcttctg gtaattcccg ctggattact cgttttattt tttctaattc gttcatcaag
     7501 ttcactttat tttgcagtcg gcctgcagta tcaactagta aaacatctgc ctgctctgtc
     7561 tttgctcttt ccaaagcatc gaatacgaca gctgctggat cgcctcctgc attgccacga
     7621 acaacttcaa caccagcgcg ttcgccccag acaactaact ggtcgattgc tccagcgcgg
     7681 aaggtatctg ctgctgccat caatactttt ttaccttcgt ttttgaactg atgggcaagt
     7741 ttaccaatac ttgttgtttt ccctacacca ttaacaccga caaacaaaat aactgttagt
     7801 ccgtcttctt ggatatttag ttcatttact tcattgattc cttccgcttc atagagatcc
     7861 acaagtttct cgatgatcgc attttggact tctgccgatt ttttcgcatt tctgagtttc
     7921 acttcttgac gtaatgcttc ggtgattcgt atcgctgttt caaaacctac atctgcaccg
     7981 ataagtgttt cttctaattc ttcaaaaaag tcttcatcga ctgttcgaaa attggcaaac
     8041 aactcattca tccgttcacc aaatgtttta cgcgtctttt ctaaaccctt gtcgtacttc
     8101 tcctgtactt caacttcttc tgttttctct tcgccagtaa aagcacgctt gattttatca
     8161 aaaaatccca tttgactccc cctactctaa tacaaatttt tggatcactt tggctacgcc
     8221 attttcttca ttgctggctg tcacgaaatc tgctgcttct ttgacgaaag gcacagcatt
     8281 ctccatcgct acgcctagtc cagcatattg gatcatcggt aaatcatttt cctcatcacc
     8341 gatcgccatg atttcttctt gtttcaaacc aagatctttt gccaataaag aaataccata
     8401 agcttttgta acaccttttg gcataaattc caaaagcata cttcttgttt tgatgatctc
     8461 atacatttca tggaattctg ctggtatttc tttgatttta ggatctaatt cttcttggtc
     8521 ataagcgatt actgccttat taagcacaaa ttcatctgta atatcgctta tatccgctgg
     8581 ttggaattgc agcagcttat tcaacacatt gtacaaggat tgcttccttg gggcactagg
     8641 cagctgtaaa accacattgt cagacaaaac atctagtggc agatccaatt gctgagccag
     8701 tttgtataag cgttggatat ctgatacttc catcactttc ttttccatga tttcaccagt
     8761 atcatttttt tgtaccaaac cgccgttgaa agtgatgctg tagtcacctt catctaccag
     8821 tcctaattct tgcagataat gcgccatcgc agctaagggg cgtcctgtac atagaacgat
     8881 cttgacccct tgctgttttg cttgtgctaa tgcttgttta ttctcatctg aaatcagctt
     8941 ttgttcattt aataatgtac cgtccaaatc gattgctact aatttgatca ttttcgttcc
     9001 tacactttcg tttcgttgat tttcgcctct tcattgatat cttccatccg tacagaaacg
     9061 atttttgata caccggattc ctgcatcgtc actccgtaca gtacatctgc tgcgaccatc
     9121 gttcctttgc ggtgtgtgac tacgataaat tgtgtgtcat cttggaaatc gctcaagtaa
     9181 cgaccaaaac gtttcacgtt ggcttcatct aacgctgctt ctacctcatc taagatacaa
     9241 aacggaacag gacagacacg gataatggaa aagagcaacg cgatggctgt aagtgctcgt
     9301 tctcctccag aaagcaaact tagactttgc agttttttgc caggaggttg tacttcaatc
     9361 tcaatacctg ttttcagcaa atcagttggt tccgtcagta ctaattcagc tttaccgccg
     9421 ccaaacatat ttggaaagac aattttgaat tcttgacgga tagcttcaaa tacttctttg
     9481 aatcttgtcc gcacttcatc atccatctca tccatcgttt caaacaattg gtttttcgct
     9541 gctaacagat catctcgctg tgttgctaag aatgtatgtc tttcactgac ttgttcgtat
     9601 tgttcgattg aatttaagtt tactggtccc agtttttcaa tctgttcttt taaggatgaa
     9661 acctttgttc tactgtcttc gatatcggtt gtttcttgat agtcttggga agctttctca
     9721 aatgtcagct gatattctga ctgcagatag gtcaaatgat tatctaaaat catttcagct
     9781 cggtcttttt ggacttctaa tttggattgt tccgacagtc gtgctttttg ctcacgatta
     9841 cgctctgcca agactgcttc aagcttatcg atctcctttt gccttctatc tcgctgctct
     9901 ttggcttttg ccaactcggc ttttaatgtt tctcgctgag cagataattc gttaatttgt
     9961 ttttccaaac tttcttctgt gacttcatga tcagaaaagt ctgctgtcaa tgtcgccaac
    10021 tgcttttcta tcgcttcttg acgttcggtt gcctctgctt tttggactcg ggcaccgcgg
    10081 agctggatct gcagatgatt aaactgctct ttcaagacag caagatctgc ttgttcttgt
    10141 gctttttgac tttgtacttg tgctcttctg gcttccatct ggtcgctttc ttgacttaac
    10201 gatttgattt cttcgtcgat tttttgtcgt tgcgattcaa gatctgtttg tttgtctttc
    10261 aattcgttcg tttgcttttc gtaatcttcg ataaactgct gcagttcacg tgtctcaaaa
    10321 gttgagattt gcttttcttt ttcaaaacgt tcaagttcat ttatgatgtt ttgtagctga
    10381 tttgtcgctt cttgttcttc aaaacgtaat tgttcgcctc gtgtacgcaa aacttcttgt
    10441 tcttcggcta aacgagctgt ttcttgttgc agctcctgaa cttttttttc ttgtgcttgg
    10501 agttgtttat ctgcttcttc aaattcacta gtcaactgtt tcaattcttg attttgtaca
    10561 aacaaactgc cttggtttcc tcgtttattc gctccacctg tcatggatcc tccggcattc
    10621 atcacatcac cttctaagga aaccactcga tattgatagc ggatcgtttg cgcgattgca
    10681 ttggcactag ttaagtcttt ggctaaaagg atcgttccta gcaggttttg aaccacagtt
    10741 tggatttgat cgggataact gacttgttca ctggcaatcc ctataaaccc ttctacagct
    10801 gctgcttgtg tcaatatatg agccggtaat tgtcttggtt tgattgtcgt cagtggaagg
    10861 aatgttgctc ttcctcctct ttgctgtttc aaatacgtga tggcttgccg agcatctttt
    10921 tcattttcaa cgatgacatg ctgggctgcc ccacctaaag ctgcttcgat agctaacgtg
    10981 aaatctgcag gaacatcgat cagttcagcc actgctccga caatacccga aagctgctgt
    11041 ttatgctgca agaccaatcg aaccccttgg taaaaaccaa aatagttttc ttggatttct
    11101 tgaaggcttt tcttacgagc acgtagctgc tggacttgat tcattaattg atacatttta
    11161 ggctgttctt ttgctaatgc ttcttggaac tttttcccgt tttgttggat catttctaac
    11221 ttctcttttg tttcagttaa agatgatttt agctgcgcct gcttttgtgt caattcttct
    11281 ttttgtaaga tcaatcggtc tactgatgct tctacttccg attgcttggc taacgtctgc
    11341 ttgctttttg ctgtttcttg tatatattga cgttctagat acttcagttc gtttcctaca
    11401 gctgcttgtt cttgcatcaa atcaacatat tgatctctta attctgccaa taattctttc
    11461 gttgattttt ggtatttttc taattcttgc tgtgttttta tgagatttgc ttcagctttt
    11521 tggatctctg tttctttttc tgctgctgct ttcatcaagc tttcttgaag cttttcaaaa
    11581 tgtttaacct ttttttgtgc ttctgctaat gaagtttgat attcttgcga gcttttttgt
    11641 gtatgctttg tccgctcctg caaaacatct ttttgtcctt ctgtctgctt cagtttttcc
    11701 gaaagatcta ataatacttg ctgatttttt tcgatcagac gatcggcttg cgcattttct
    11761 ttacgctgct tcgccaaaat ggattcttgt tcttggatcg actcagatag tttgcctaat
    11821 tctagattga atttcgcaag ctgtgcttgt ttgttgtccc aatctttttt tgctgtcttg
    11881 atttcagcaa ccatcaaaga gacatcggtt tgagtcagtg tttcttttag ccgtaaaaac
    11941 tcctttgctg cctcactctg ggcggccaaa ggagttaact gttcttccaa ttcgtggatg
    12001 atgtcttgga cgcggctgag gttgtcttct gtttcaaaaa gcttctgttc tgctttcttt
    12061 ttccgctgct tgtatttcaa gacaccagct gcttcttcaa agatcccacg acggtcttcc
    12121 ggtttactac tgaaaatcgc ttctactttt ccttgagaaa tgattgagaa cgattctttt
    12181 cctaatccag aatctaagaa caattcttgg atatctttta atctgcagct ttgtttattg
    12241 atgaaaaact cgctttcccc agtccgtcga taacggcggg ttacactgat ctcattgtac
    12301 tccaatggca gataatgatc actattgtct aaaatcactg tgacttctgc aatattcagc
    12361 tgcttacggg tatcggaacc ggcaaagatg atatccggca tttttccgcc tcgcagactt
    12421 ttagcagatt gttctcctaa tacccagcgg atagcctctg taatattgct ttttccgctg
    12481 ccatttggtc caaccactgc tgtgacgcta ttttcaaaat caatgactgt cttgtctgca
    12541 aatgatttaa aaccggctat ttcaattcgt ttcaaataca aagctttccc tcacttactt
    12601 ttacagtgct gccaatgcat tttccgccgc agcttgttct gctaattttt tcgatttccc
    12661 ttgtccagtg ccaatcaatt gatcatctac ataaacttcg atccaaaata ctcgttcatg
    12721 agctggacct tcttcattga tcaatcggta ttcgattgat acatcacctg atttttgcaa
    12781 gacttcttgt agttttgttt tatgatccat ctcatgtgaa aaagcacctg ctttgacttt
    12841 aggaaaaatc actttttcga tgaatgcatg ggtcgtgtcg aatccctggt caagatataa
    12901 cgcgcctaaa aaagcttcga ataggtcgca aagcaatgct ggtcgcgtac gtcctcctga
    12961 attctcttct cccttgccca acaaaacata ttgatcaaag tgacattctt tggcaaattt
    13021 cgacaagctg tcttcgcgaa cgatcgcagc tcgtgttttt gtcaattttc cttctggcaa
    13081 atgaggatat aaacggaata aatactgaga aaccatcagt tctaaaacgg catctcctaa
    13141 aaattcaagt cgctcattat ccgataattg cagattgcga tgctcattca cataggatga
    13201 atgggtaaaa gcttgttcaa gcaaatttag atcgtgaaaa acgatgttat agttttcttt
    13261 tagttctttt gtcagctggt tgtccatttt ccacattcct tttatggata ttttttcgat
    13321 acttccttat tatactggtt ttttgtcaaa tttgaaatgg ttcggtcaaa aggtaagtaa
    13381 agctttaata atcctagtga ttgacgagaa gttgtgaaaa attcgtttag tttcgagtat
    13441 gaagaaaaaa ttttgtgaaa atggctttcc atatttttgc ataaatttga cttaataccg
    13501 agaaactgga ctttgaacac cgtttattgg agaagtcatg aaatcagcgc cttgacctga
    13561 atattagaga aataaacaga aaaatatcaa aaaaccgaaa aaggacatcg ccctttttcg
    13621 gtcattttta gtgattatga acgtgaatct gctgtaacag aaacatttgc ccatgggttt
    13681 ggtacatcat aagaccaatc ccagtctttg acacggtcgt tgactggcaa tacttcatta
    13741 cggaacaacg ttggaatcgc aaatgcttct tcaaatgcat attgttgcca tttttgatat
    13801 gcttcttttt ggtactcttc atcaaatgct ttgcttgaac tgatatcgtt gatcaatttt
    13861 gtattttctt ctgattcata acgagtgtag ttgaacgcag catttggtcc ccatagtcca
    13921 cttggatttg gatcagttcc tgtattccaa gcagcttggt aaacatcaat ttcaggatca
    13981 tcgttttcta atttatcata gaaagaattg aattcgatca gacgacctgt tgtgtattgg
    14041 acatccaaac caatttcttt ccattgttgg atatagtaat ctgccaatgg ttgcgctgtt
    14101 tccccaccgg acatcgaagc aaagttgatt gttagtttat ttccatcttt gtcttctctt
    14161 aaaccatcgt catccacatc tttgtagcca gcatcatcta atagtttttt cgctttgtct
    14221 agatcttgtg tgaatccttt catctcatca tcatggtatg actggaatac tggaggaatc
    14281 aatgaagtcg cattgcttct caaacctgca tagaaacgtt cacctactgc attattatct
    14341 aatgcatagc ccattgcttg acgtaatgat ttatcagcca tttttgaatc tggattgtat
    14401 tccacactct ttgtatctgc attccattta cctaacttga atcctagata agtataagaa
    14461 gtttgttcac ggcctaagtt tgtatagcca gcaatttctt tataggtatc gtaagtatct
    14521 gttggcattt ggaaaatcat atcatataat ttagagttca atgcttcagt tgcacttgcc
    14581 atcggtacag atttcatcgt gatctttttc aatttaggcg taccattcca gtagtgctcg
    14641 tttggcaagt attctactga ttctcctgca acgatattac tcatgtaata agggccaaaa
    14701 gtcactggat ttttacgtac ttgatcgctt gattccatat ctttgattgc tacaccttct
    14761 aacgtatgtt ttggtgctgc atatgaccag ataccaccac ccgattgaag catgcttggg
    14821 tttacttctt tatattcgat ttcaactgtt ttgtcgtcta tttttttgat tccggagatt
    14881 gtgtcggcac ttccatcatg atattcttcc atacctacga tgttacggaa agtgtcgtca
    14941 taacggatac cagtgtaatc tttattccca atgatttcgt aagggaaaat cacatcgtcc
    15001 gccgttacat cttcaccatc tgaccatttg acgttgtctt ttactttgat tgtcgctttt
    15061 ttcgtatctt catctagatc aagcgttgca gctccgtcgt ctgtgattct gaaatcgtca
    15121 tcgtttgcaa atagcggttc atgagaagga gccataaaag ctgcatcata tgaatcttgg
    15181 taaaactccc actggaacaa tcctttaaac tgactgtccg ttgcgaccgc agcttctaat
    15241 tcgccgtctt cgattgcatc atcattattt tttgttgcaa ttggaaaagt taattcctct
    15301 ttgttattgc cgttgcctcc agagttgttt ccagaggtat ctgtacctcc gcccccgcat
    15361 gctgcaagaa cgacagcaga catcagcgct gcaaatccaa aaatcgtttt cttcattcct
    15421 atttccccct tcgcttctat actagcctaa ccgttggcgc gcatctgcag aacgcttgaa
    15481 tgcttgtcca acataattga tactcaacat caaaactaaa ataaggattg atgcgggtaa
    15541 ccagatccat tgccgattca ctaagacttc tccgttactt gcataaccga tcaatgtacc
    15601 taaactaggt acatttgccg gcaagccgaa acctaagaaa gtcaaagttg tctcgatacc
    15661 gatgtttgcc gcaaagttca tcgtcaaatt agtaatgatt aatgaactta gattcggcat
    15721 gatttcgcgg aacatgattt taaaatcatt ggttcccatt gtttttgagg cactgacata
    15781 atctcttctt gcttcagata atgtcttgct tcggaataat cgggctttag ctacccaata
    15841 aaatgcactc atgatgaata taaaggacca gacattgtat ctggaaataa tcgatacgaa
    15901 gacgataatg atcagcatga tcggtagaat catgataaaa tcaacgatcc gcatcaagat
    15961 gttgtcaatg atccctccgt agtagccaga gatgatccct aatccaaccc cgatgatcga
    16021 agtaaggatc gtgatcgcaa atccgatcgt aacagagttt ctagcaccta tgatcaattg
    16081 tcctaataca tcacgtccgc cttcatctgc tcccagaatg aacttttgtc cttgtgtcga
    16141 aactgttcct ggtgcagcgt atttgtcgaa gatactaaca tacatcactt tgtcttgatc
    16201 ggtcagcatc gcaccgataa agatcactaa taataaaacc actagcaaac ctaatgaaaa
    16261 aatagcaatt ttatcttttt taaattctcg agcaatcatt cgtatcccca taggaggaat
    16321 actttcttga actgctgtat tgtttttttc actcatcgat ccattctcct ttctgattgg
    16381 ttttattgaa tccgaatacg cgggtcaacg atactcatga tgatatctga aagtaatgtc
    16441 cccactagcg tcgcgattcc taaaatcaat actaaggatg tgatcacggt atagtcccgt
    16501 tggccgatgg aatcaataaa tagttttccg actcccggat aaccaaaaat cttttcgata
    16561 actacagaac cagcaatcaa cgaagtgatt tcatacccca tttgagaagc catcggcaac
    16621 gctgcatttc ggaaaatatg tcgactgaat actttattcg tcggtacccc tttggaacgg
    16681 gctgttctga caaaatcgag cgatttcgaa tcgattactt cacttcgcag atattggatt
    16741 gtgacggcag tcgccaaaaa tgcttgggta attgatggta acagaatata tttcaaacgg
    16801 tctatccatt gagtgataaa tccaccttgc aaacctagtg tttgtgatcc acttgttggg
    16861 aaccagccta aacgataacc aaaaacaaat agcatgatca agccgaagat aaataaagga
    16921 acagcaaaac taaagaagtt gtagattacg acaatcttat ccaagattga attttgatac
    16981 cggccagcaa gcatccccag aggcacagca atcaaataag taaggacaac tgtcaagatc
    17041 gatagatata aagtgttgat gatccgttcg ccgatcaagt cagcaacaga gcgtttgtat
    17101 aagaaacttt gaccaaaatc gccttgcaaa gcatttccga tccaacggaa gtactggaca
    17161 taccatgggt cattcaagcc agctgcttgt cgcatctgtt cgatcactgc cggatcctga
    17221 tttggattga tcaaaccggt aaatggatcc cccggcatcg cttgggctaa taagaagatc
    17281 agaacactta aaataataac ttgcgggatc atcaataaaa cacgtcgcag aatcgttttc
    17341 cacattagct tgcacctcca tctttcaacg ccactttgtg tgtagttgtg atcgtacgca
    17401 aatcgtagac tcgaccagtc gcatcatagt attctttttg gttttggata tactcttgtt
    17461 ctactcttcg tcgattttct ttgtggcttt ccctattttc tacatcgatt tgcggaatag
    17521 ctgaaagcag acgtttcgta tagatatgtc gcgggtcatt ataaatgtct tctcttgtac
    17581 cgatttctac aaatcgacct ttgtacataa ttgcgatatt gtcgcacatg tgttttacta
    17641 cacctaaatc atgtgaaatg aataaatagc tgaggccgta ttcttgctgg attcgtttca
    17701 tgaaattcaa tacttgcgct tgaacagata gatccagagc agatacaggt tcatcggcta
    17761 cgatcagttt tggattggta gcaacagcac gggctactcc tagacgttga cgttgtccgc
    17821 ctgaaaattc atgaggatat ttatatagcg catcttccgg catcccaacg atatctagca
    17881 gtccttttac ctttttcttt tcttcttgat cgcttaaccg ttcaaaattt cggatgggtt
    17941 cagcaatgat atccaacact cgctttttag gattcaaact ggacattgaa tcttggaaga
    18001 tcatctgcac atccttgtta taacccattt ttttgcggtt gcttcgcttc gttacatctt
    18061 ttccttcata catgatttgt ccggctgtta ccttttctaa tcccacaacg gcttttcccg
    18121 tagtagattt tccagagcca gactctccca ctaaaccata tgtctttcct ttttcgataa
    18181 taaaatccac accatctaca gcatatacat gatccgttac tcgattgaag aatccactgc
    18241 gaatagggta atgaactttc aggtctttga ttgtgattaa ttctgtcatc tacacctctc
    18301 ctttttcgtc tctaaagtgg aaatgcttat agcaggagca acgtaccaag tggttcggtg
    18361 cgacttcatg aaggataggc tcttcttcat gatcgcttgc tggtatccat ggaatacgtg
    18421 gtgcaaaccg acaaccttta cgaggcatgt ttttcagaga aggaacgaca ccttcgatca
    18481 catggagttc tgcatcatga tttccttctt gtggtataga gttcagtaaa gagcgtgtat
    18541 aaggatgctt aggatgatca aacagttctt ttacacctgc tacttcaacg aactgtccgc
    18601 catacatcac tgccactcga tcagccattt ctgctacgac tcctaagtca tgagtgatca
    18661 agatgatccc tgtttttgtt tcttcctgaa ggtctttcag taaatcaaga atctgtgctt
    18721 ggatggttac atctagggct gtcgtcggtt catctgcaat gacgattgga ggtttgcagg
    18781 cgattgcgat tgcgatgatg acacgttgtc tcataccgcc tgatagttca tggggatatt
    18841 ggcgtccaac tctcgcagga ttaggaatcc ctacttgttc tagcaattct agtgcacgag
    18901 cttgacgctg ttctgcattc atctttgtgt gataggaaag tccttctttg atctggtctt
    18961 cgattctcat gagaggattt aatgcagaca acggatcttg gaaaatcatt ccgatatcat
    19021 ttccgcggat tttattatag agcgtttcgt tcaggtcgat taaattcaga tcgttatata
    19081 aaatctcacc tgtaattttt gtgttattag gatcaagtaa tcccataatc gtagtagcca
    19141 aggtactttt cccgcaccca gattccccaa cgattgctaa aatctcgttt ttgtctaaag
    19201 taaatgaaac gtcatctact gcatcgtaat agtcatcttt gagtcggaat cctgtatgta
    19261 ggttctgaac ttctaataat tggttatcgg acaatcgaat acccctctct gcttcctaaa
    19321 tggaagtttt tcttaataaa aaaggacatt acacagaata aatcgt
//
LOCUS       DAZKMK010000053        19027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000053 DAZKMK010000000
VERSION     DAZKMK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.053"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..2195
                     /locus_tag="ACG8V1_001749"
     CDS             42..2195
                     /locus_tag="ACG8V1_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0329844.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2164..3579
                     /locus_tag="ACG8V1_001750"
     CDS             2164..3579
                     /locus_tag="ACG8V1_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0329845.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3612..4646
                     /gene="sppA"
                     /locus_tag="ACG8V1_001751"
     CDS             3612..4646
                     /gene="sppA"
                     /locus_tag="ACG8V1_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0329846.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4648..5169
                     /locus_tag="ACG8V1_001752"
     CDS             4648..5169
                     /locus_tag="ACG8V1_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0329847.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5166..5552
                     /locus_tag="ACG8V1_001753"
     CDS             5166..5552
                     /locus_tag="ACG8V1_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0329848.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(6048..6120)
                     /locus_tag="ACG8V1_001754"
     tRNA            complement(6048..6120)
                     /locus_tag="ACG8V1_001754"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6085..6087),aa:Lys,seq:ctt)
     gene            6501..7070
                     /locus_tag="ACG8V1_001755"
     CDS             6501..7070
                     /locus_tag="ACG8V1_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0329849.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7057..7917
                     /locus_tag="ACG8V1_001756"
     CDS             7057..7917
                     /locus_tag="ACG8V1_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0329850.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8107..8811
                     /gene="yycF"
                     /locus_tag="ACG8V1_001757"
     CDS             8107..8811
                     /gene="yycF"
                     /locus_tag="ACG8V1_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0329851.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            8816..10651
                     /gene="walK"
                     /locus_tag="ACG8V1_001758"
     CDS             8816..10651
                     /gene="walK"
                     /locus_tag="ACG8V1_001758"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0329852.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            10648..11964
                     /gene="yycH"
                     /locus_tag="ACG8V1_001759"
     CDS             10648..11964
                     /gene="yycH"
                     /locus_tag="ACG8V1_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0329853.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            11965..12834
                     /locus_tag="ACG8V1_001760"
     CDS             11965..12834
                     /locus_tag="ACG8V1_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0329854.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            12889..13698
                     /locus_tag="ACG8V1_001761"
     CDS             12889..13698
                     /locus_tag="ACG8V1_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0329855.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13801..14430)
                     /locus_tag="ACG8V1_001762"
     CDS             complement(13801..14430)
                     /locus_tag="ACG8V1_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0329856.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            14600..15892
                     /locus_tag="ACG8V1_001763"
     CDS             14600..15892
                     /locus_tag="ACG8V1_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0329857.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(15968..16630)
                     /locus_tag="ACG8V1_001764"
     CDS             complement(15968..16630)
                     /locus_tag="ACG8V1_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0329858.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            16855..17139
                     /gene="groES"
                     /locus_tag="ACG8V1_001765"
     CDS             16855..17139
                     /gene="groES"
                     /locus_tag="ACG8V1_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0329859.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            17190..18815
                     /gene="groL"
                     /locus_tag="ACG8V1_001766"
     CDS             17190..18815
                     /gene="groL"
                     /locus_tag="ACG8V1_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0329860.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6473 a         3066 c         3939 g         5549 t
ORIGIN      
        1 tttttctata aaaatttttt agaaggagtt gtgttagata agtgataaaa tcatttcctt
       61 ttgtgcagca acaagatgaa aaagattgtg gtgttgcttg cctttcgatg attttaaaat
      121 attttaaaac tgatatcccg attcataaat tacgggacct atctgggact gatttagagg
      181 gtacttcagc atttggattg aaaaagacat ttgaaaaatt gtcttttgat tgtatggcta
      241 ttcaagcaga tagtgagatc tggaaagaaa gagatcttat cttaccatta attgcacata
      301 tattggttga taacagctat atgcactacg ttgtaattta taaaacgaag ggaaacgtac
      361 tatggattgc agatcccgct aatggaaaaa atagaaaaac gattgaagcg tttcaaaaag
      421 aatggacagg tattctttta ttacctatgc ctagagaaac ttatattcct attaaagaaa
      481 aagtagctgg attaagttct tttttgccta taatttggaa acaaaaagga ttagtattcc
      541 acatcgtttt agcatcgatt ttcattacgt tttttggcat aggaagctca tattattttc
      601 aaggtattct tgattttttc atccctaatc aagcacgttc aactttaaat ataatttcca
      661 ttggcttgat cgctgtctat ttatttcggg tattgtttga atatagtcgt agttatcttc
      721 ttattatttt aggacagcga atgagtatgg caattatgct tcagtatttc aaacatgtgt
      781 taactttacc aatgaatttt tttgctacga gaaaatcagg ggaaattatt tctcgttttc
      841 ttgatgccag taagattatt gatgctttag caagtgctac gttatcaata tttttagata
      901 ttggtatggt gttgcttgtt ggggttacgt tagctattca gaatagtacg ttgtttttaa
      961 tcactctatg ctctttgcct ttttatgtag ttgcaatatt cgctttcgtg aaaagctatg
     1021 aaaaatcgaa cagggaagaa atggcagccg gtgcagctgt aaattctagt atcatagaaa
     1081 gtcttaaagg aatagaaaca attaaagcat atagcggtga agagaaagta tataaccgta
     1141 tagaccatga atttgttaaa ttaatgaaaa aatcatttcg tacggttacg cttgataata
     1201 ttcaacaagg aattaaacat ttgattcaat taattagcag tgcattaatt ttatggctag
     1261 gttcttattt tgttatggat aatagaatta gtctaggtca acttatcaca tacaatgctt
     1321 tactagtatt tttgacagat cctttacaaa acattattaa tttacaagtg aaaatgcaaa
     1381 cggctcaagt tgcaaataat cgtttaaatg aaattttcgc gattgattca gaaaaatcga
     1441 accagtcttt tgaatatatg ctttctgaac aattatttca acaagatatc actgtaaaaa
     1501 acgtttcatt ctcgtatagc atgaaggccc caacgttgaa cgaaatttca tgtacaattc
     1561 cttcgcacag taaaacggct atagttggag ttagtggttc tggaaaatca actcttgcaa
     1621 agttatttgt tcgtttttat gaagcatcag aaggatcgat ccattatgga aacattaatc
     1681 acttagacat tccttttcag atattacgcg atcgagtgac atatttgcct caagagtcat
     1741 ttttctttag tggaacaatc attgagaatc ttatattcgg tttagatagc ccaccaagtt
     1801 tcgaacaaat aatagaagtt tgtgagatag tccaactaaa tgaatttatt aatcaacagc
     1861 cattacgtta cgagacattg ttggaagaag gtggaaataa tctttcagga gggcaacgac
     1921 aacgattagc cttagcacgg gctttattaa aaaatccgga catattaatt ttagatgaag
     1981 caactagtgg attagatacc ttgttagaac atgcaattat gaattatctc ctcaatttaa
     2041 aagaaaaaac gattattttt attgcgcatc atttaccaat agcaaaagca tgtgatcaaa
     2101 ttttagttct ccatgaaggg catttggtag aaaaaggaac gcacgatgaa cttcgttttg
     2161 ccgatggtat ctatcaaaaa ttatgggaaa tctaggagag ggaaacaatt tatgcagaat
     2221 aataattggt tcgaacattc tagtatctat actaaacaac atcatagctt ctaccgttgg
     2281 gtgttatacc ctgtaattct ttttttatgt attattggat tgtttttaac ctttgctaaa
     2341 aaagaggttg ttattcggac aacggctcaa ttgaaccccg aaaaaataga gaagttacaa
     2401 gtgccattag aagctaaaat aatggaaaac ttcctaaaag aaaatcaatt tgttcataag
     2461 gacgatgtgt tggttagatt ggattgttct ttaatagaga atgaaaaggc tcagatagaa
     2521 caagaaaacc aacgtattac tcaacaaata aagatggctc agctatttat tgaaagtata
     2581 agtaaaggaa aaaatttgtt ttcaacggat gacagttttg gctacagtaa tcaattaaag
     2641 agcatgttgt cagaaaaaga atcactccgc tacgctttga agcaaagtga attaaatgat
     2701 caaaagcaat tagaagtata cgaaaagaca aaaagacaac tagaaaaaca aattgagagt
     2761 tcagatagta aattacaaga atggcaacaa gtacaggtag cttggagtaa taatcaatca
     2821 ttaaaagatt tttcaaaaga aatgatggca aactatgaga attggcaaga acaactaaat
     2881 aatgtttctg atgatcaaaa aaatcaagtg aaactgacaa tttcagcaag cataaatgaa
     2941 caaattgagc aactaaaaaa agaagtagaa cagtatcagt cagaaaaagc taaattagtt
     3001 aaaccaacta cttctgagaa tgacagaatt agtcaaacgg aaaaaggaaa gcaagagcta
     3061 gaacaagcaa taacaactgt aaaacaaaca gttacagagc taaatgaaaa acaagagaag
     3121 aaccagtcag ttatcaaaag tttggacgat caactttcaa aaggaatact aaaagctcct
     3181 gtatctggaa cggttcattt aaatgaagaa acaaagggac aagcagaact agccaaagga
     3241 acagtgcttg cagaaattta tccgatccac gaaaaatcaa aaatgaaatt tacagcactt
     3301 ttacctgcga atgaaagtac ctatattaaa gaagggatga aggttcattt taaattggat
     3361 caaaaaggtg gggctccgat tacgatcgac ggaactttag atgaaatttc agaaaattct
     3421 actgccactg agaaaggtgt tttttatgca gtgaaaggaa cgttaaaaac aactcaatcg
     3481 tttccgtttc gttatggatt aactggagag ttgtctttaa ttgtaggaga aaaaacatat
     3541 tggcaacaaa ttgtcgatgt gtttctaaat aaaaaataat tatttttact aagaatatag
     3601 gaggagttta tatgaataaa agaagatgga ttgctgtggg agtagcgtgc ggattattgt
     3661 tactatccat tatcgtcgcg ctaattccag gaaaagataa agaggaagca tcaaatacaa
     3721 ccctaacagg aataaataag attttttacg gatctaacga aatcacagaa gaaacgttag
     3781 aagaaggcgc atcaaataaa aagattgtca aactatccgt caatggagtt attgcagata
     3841 caggtgaaag caatcttttt agtagggaac aatataacca tcagaatttt ttaacacagc
     3901 taaagaaaat tcaagaagac aaagcggtta agggagtgtt attagaagta aattcacctg
     3961 ggggaggaat atatgaaagt gctgagattg ctaaagaaat ggcaaaaatt aaaaagctag
     4021 atattcctat ctatactgca tttaaaaata cagcggcaag tggagggtac tacatttctg
     4081 ctggttcaga taaaatattt gctacggaag aaacaacaac tggatcaatt ggtgtcatta
     4141 tttctggttt aaattattct ggcttattag aaaaacttgg cgtaactgat gcgacgtata
     4201 aaagtggtgc attaaaagat atgatgtcac ctcaacacaa accttcagag gaagagaata
     4261 aagttataca ggaatttgtg atgagtgctt atgatcgttt tgtcaatgtt gtagcaaaag
     4321 gaagaaatat ggatactaat gcagtgaaag aacttgcgga tggaagaatt tatgatggta
     4381 accaagcagt agaaaatggg ttggtggatc aaattggtta ctctgaagat gcattagatt
     4441 ccctaaaaaa agagaagaaa ttaactgatg cgacggttat agaatataaa aatgacacaa
     4501 ctggctttgc ttcttcttgg ttaggtaata agattgctga atggcaaggg ctaaaggcaa
     4561 ctacatcaga tcgtgtaatt tcagtttttg aaaaacttgg attttctgat actccaaaac
     4621 caatgtatta ctatggaggg ctataaaatg aataagaaga gtggcattga aactttgcct
     4681 aattatgtat ttgcgggatt ttggttacga ttttttgctt ttcttattga tttaatttgt
     4741 attagcatca tcacccatgc tactataggt ctcttttaca aaatcatggg actggaatct
     4801 gacaaatcca ttattacttt ttatggtata gcgtcattag ctatttattt aacctatttt
     4861 gtacttttga ctaagctgaa ttcaggacaa acaattggga aaatgatttt tgggataaga
     4921 gtagtgagct ttgaggaaaa agagctaagt tggcaaacta tctttattcg tgagtgtata
     4981 tgtaggttta tattaaaaaa ttttgtattt caaattgggt atttggtggc tgctttcacg
     5041 cccaagaaac aacatattgg ggattatttt tctaacacgt cagtggttgt tattcgttca
     5101 ttagaagcta ttaactatgg tcaaaaaatt tctaattcta aaacacttaa tctggagaga
     5161 attaaatgaa taagagaaaa acagagttta ttttgatttt aattagtgga ataacaggga
     5221 ttttatgtag tcttttagca ttgatgggtg gtatgtttct atcttctatt gattcaacta
     5281 ctttaaatac tacagaacta accagtaagg atatagatat gttagtcaat ctaagcggga
     5341 atgttgtttt atttggagga attaccctag ttttttcgat aatgattttc ataataggtt
     5401 ttttagtaag aaaaaatcaa aaaacagtgg tctatggtat tagtttactc attttatcta
     5461 ttattccatt ttttctttta acattattgt gggtaattcc tggagtttta ggtattattg
     5521 cgggattgat gcttctaata agaaaatctt aattaggcaa tgtgtagaca acgtgaaacg
     5581 attaatcaat taaaagctaa atacgttttg agaaatgata acttgtagtc gatggaagac
     5641 atgtagataa aaaaactacg tgtcttttat ttattctaat tttttcgaaa aaaagacact
     5701 ctctgattgt tgtattaatg gaaaagggga aagcagcaag taaatttagt ttttctatta
     5761 aatattcttc actttttaac cttttatagt acttagattt aatcctatat gtttagagag
     5821 ttcattggca gcatgatttt gcaaagcttc tcgaattatt gttatattag aaactttact
     5881 gttcataatt atgcttcttt tttaactcaa agtccaaagg gctacagtgg actttgagtt
     5941 aaaaataaaa agccctagtt attttgaaaa ataaaaaaag aacagcgtaa atgttttcga
     6001 aatgctttcg caccaacatt tacctgttcc gtgggtttgg cacccaatgg gccgtgaggg
     6061 gctcgaaccc gcgacccgct gattaagagt cagctgctct accaactgag ctaacggccc
     6121 gtacaaaaag tacttaataa tagtagcact aagaatttta aaatgcaaga ctacatttaa
     6181 agaaaatcag aaaaatcttt acttccttat aggaaatcat tcatagatta ttcacatttg
     6241 ctcgctatta tataagcata ccaacaaaca aagttcttgc aagagaacaa accctaacac
     6301 ttttttcatt ttttactcct ttcccgccgt ctccccggcg ggtctcctgt tcttaacgat
     6361 aaaagacaag aaggcttagc cttcttgttt tttgttttca tggaatcccg ctaatcaaat
     6421 cacctagtta aagcaactag attgaatgtt tatcataagt ctgatatcat gtttatgaaa
     6481 tgaaaaaagg agcggttatg atggaaaaaa tgcgtcagca attaatggag tgggggaagg
     6541 aactagaaac attccgtctg cctcactggg aagaactccc tgacttagaa ctttatatgg
     6601 accaagtcag aacgttagtt gatcgttatc tttcacctgt gatacaagga gaaaagcatc
     6661 cattgcttac ctcttctatg gtaaacaatt acgttaaact aggtttgata ccagcaccag
     6721 ttaaaaaaag atataacaaa gaacacgtag catttttact tgcaatcact acgttgaaac
     6781 aagtattgac tattcctgaa atcaaagaag ggatattgtt ccaaggaaaa acagtaggaa
     6841 ttcgtgaagc ctataatcta ttttgcgatg aacaagaagc agctgtgtgg atggtcagcc
     6901 aattagcaca aggtaaatct catccgcaaa aattcgacca ggcaacccct gtagaatata
     6961 ttgctgtaag agcggcaact ttatcgtttg caatgaaact tcttgcggaa aaaacgattg
     7021 ttctagaaac cgaatattta aaagaggaga aaacaaatga aaaacaataa aatcgcaatt
     7081 ttagtagatt caggtacgga tgttccagaa gaattgataa aaaaatacga gatgtatatg
     7141 atcccattga aaattattta taaagatcag gtatatacag ataaaattga cattacccct
     7201 gaagagatct atcaacgttt gccagaggaa atccctggta catctttgcc ggatggtgag
     7261 tcgatcacag aaatctttga gcaaatcaaa aaagacggtt atgaaaaggt acttgcggta
     7321 acaatttcca gtggattgag cgggacatat aatgtggttc gtttaattgc acaacagcaa
     7381 gaagaattgg aagcatatgt actagatacg aaaaatattg gtattggtgc aggtttttct
     7441 gcgatccaag cagcaaaatg gctggaagaa ggagtagaat gggatcaact gatcagtaat
     7501 ctgaatgagt tagtcgaaag aacaaaagtt ttctttaatg ttgcgacatt ggaatatctt
     7561 caaaaaggtg gacggatcgg tttagttgct tctattgtag gaaccgcttt aaagcttaac
     7621 ccaatcatct catgtaatga agaagggatt tattacacag taggaaaagc taggggacgt
     7681 aaaaaaagtt tggataaaac ggttgcttac gtcaaagaaa aaatcggtaa tgcaaaggtc
     7741 ttcaacttag ctgttgccca tggggatgcg aaagaagaag caattgaaat ggaagcgaga
     7801 cttaaaagag agttcccaca agcaaaccaa atctactttg gccagatttc tccggcatta
     7861 gtagtccaca ctggtccggg attattgggt gtcggtgtac aagttttaga aggataaatc
     7921 aaaaaaagga aacactgagg tgaaaatcag tgttttcctt ttttattgaa gttagaacaa
     7981 aaaataataa agtattttgc actttcattt taattttctc ttttgtttta taggcaaaga
     8041 tgaaacaaaa gcagaaatac ggtataatga gaaggtatga caataatata taagaggtgg
     8101 ctctttatga aaaaagtttt agttgtcgat gatgagaagc cgatttcgga tatcgtcaaa
     8161 ttcaatttag ccaaagaagg ctatgacgtt tatactgcat atgatgggga agaagccttg
     8221 gaaaaagttg ctgaggtaga acctgatttg attttacttg atttgatgct acctaaaatg
     8281 gacggcttag aagttgctcg tgaggtacgc aaaacttatg atatgccgat cattatggta
     8341 acagccaaag attctgaaat tgataaagtc ctaggcttag agttaggcgc cgatgattac
     8401 gtgaccaagc cattttccaa ccgagaatta gttgcacgtg tcaaagcaaa tcttcgtcgc
     8461 ggtgccacgg ctgcgaagga accagaagag gcagcacctg cggaattgac gattggtgat
     8521 ttgacgatcc atccagaagc ttacatggtg acgaaacgag gagaaacaat cgagttaaca
     8581 catcgtgaat tcgaactact cttttacttg gcgaagcacc ttggacaagt gatgacaaga
     8641 gaacatttat tacaaacggt ttggggttat gattattttg gagatgtacg aacagtggat
     8701 gtaactgtac gtcgtttgcg tgaaaagatc gaagataatc caagccatcc aaattattta
     8761 gtgacacgtc gtggtgtcgg ttactatttg cgtaacccag aacaggagta attggatgaa
     8821 aagaaaagtc cgcttttttc gatcagtcaa ttttaagatc gctattacct ttattctgat
     8881 tttattgatc tcgatcgaaa ttattggtgc ctactttata cgtggtttag aacgttccac
     8941 gatcaataca tttattaaag atatgaatca aacggtggaa tcattagcaa caacaatcag
     9001 cccggaattg aatcgaaaag ataatgcaga tgatgaagag gtaaatgcga atatcaaaag
     9061 atttattgaa aacagtgcga ctagcgatat catcgaaata cgggtcgttg acgaaaaagg
     9121 gatcatccga ggaacaaccg atgtcaatga acaaagcgct gtgggaaaga aaaatgatta
     9181 tgttgatatc aatgatttca cgactaaacg ttatgttgct ttagataacg acgataaacg
     9241 ggtgaatatc aatgttcaac cgatcctgtc cccgaccggc gatgccgtta tcggggctct
     9301 ttacgtcaaa agtaatatcg aacaaaaata ttcggaaatc aataacacag cagtcatttt
     9361 ctttactgcc tcactaattg cggcatttat ttcaatgatc gtatctgttt tagttgctcg
     9421 ttcgattaca cagccgatcg gtgaaatgag agaacaagcc attcgaatcg ctagaggaga
     9481 ctacagtcgg aaagtcaaag tgcatggaca agatgaactt ggacagctgg ctgatacctt
     9541 caatcagtta gctgaacgta ttgaagaagc tcaagataca atggaagctg aacgcaatcg
     9601 tttggatagc gtgctatccc atatgacaga cggcgtcatt gcgacagatc gcaggggtaa
     9661 agtcattacg atcaatgata tggctgtttc cttgctagat atcaaaaacg aggaagcaat
     9721 cggacaatcg attttgacac ttttagatat cgaagaagaa tatacgttgc ggaaattatt
     9781 agaagatcca aacgaaatgg tacttgaccg ttcaaccagt acattggaaa gtgatcagat
     9841 gatcttgcga gtagactttt ctatgatccg tcgcgaatca ggttttatca gcggattagt
     9901 agctgtcttg catgatgtga cagaacaaga aaaaacagaa cgggaacgtc gtgaattcgt
     9961 ctctaatgtt tcccatgaac ttcgtacacc gttgacaagt atgcgtagtt atatcgaagc
    10021 attgagcgaa ggtgcgtgga aagacgagga agtagcacca aattttttga aggtaacgct
    10081 agatgaaaca gaccgaatga ttcgaatgat caatgacctc ttaaatctat ctcgcatgga
    10141 tacaggtaat acccagcttc aattagaata tgtcaatttc aacgagttag tgaattttgt
    10201 tcttgaccgg tttgatatga tggtgggaaa tcaggaaaaa aattataaga tccgtcgtga
    10261 atttacgcaa agagatcttt gggtcgaagt agatacagat aaaatcatcc aagtagtcga
    10321 taatatcatg aataacgcga tcaagtattc accagatggg ggaacgatta cttgtcgctt
    10381 attagaaacg cataataacg tgattttaag tatcaccgat caaggattgg ggattcctaa
    10441 aaaagatctg aaccgagtgt tcgagcggtt ctatcgagta gataaagcac gtgcaagagc
    10501 acaaggtggg acaggacttg gattagctat ttctagagaa gtgattaagg cgcatcgagg
    10561 cgctatttgg gcagagagca aggaaggaaa agggtcgact ttttatatct cgctgccata
    10621 tgaaccttat gaggaggaat ggtgggaatg agaatctctg aaaaagtcgt tcgtatagga
    10681 ctcattttga tggttgcgtt aagcatatac ttctcttatg caatctggct gagcccagca
    10741 ggaaaaagtt caatcaacgt agatgagaca aacactcaaa tgatcgagtc tcaaagcaat
    10801 cgaaaaactt ccgaagtctt tcttccgctt catgttacat ggcaccattc gggaatgatc
    10861 caagaaacca acagtgaaaa tctcgtgtac cgattgcaat tgatcatcga taaagcaaga
    10921 tttggaaagc tttctgaagt agtttcagga gacaaatcac agtttgaaca ggtgaaaaat
    10981 ctgaactctg gttttgagtt ggcctataat gcgccttttt tgctaagaga atacaaggaa
    11041 gcatttgatt tagatttaga tgtgtcgaac ttggcaaaag aaagcgaaaa tatgtatttt
    11101 actagaatac aattcgatca aggaaataaa aaaatccgat ttttgaatta ttcgaaccat
    11161 ctcgtatatg aagcaaatat ttcgttgaat tggacagata tcaataaaga attaacagca
    11221 aaagacacaa aatggacaga aatgacaaca gaaccatcga cgatcgacac gcaatatgct
    11281 acgaaacatt cgatcaaact gaagaagtat agttatatcc tgtcacagca accctataca
    11341 ctttttagaa atgctttttt ccagaaacca gatagcgtga aaaataatga tgaaagcaat
    11401 gaattgattt tttacggtgg gaatgaaaca atgtctatcc attcggaaac gcaagtagtt
    11461 gattttcgtg gagagctgcc agagaaaaaa gaaaatgcta gtgtattttc tgaaagtttc
    11521 ccctatatta gcaaattagg cagttcttta gggaacctca gatattttga ccgcagtgac
    11581 aatacgatcg attatcggat attcgtggaa ggcttcccgg tttttgggac agacagcaga
    11641 ggacagattg gtgtggaagt tggcggtgaa acgaatggtt ctgtgcaagt gaatatccaa
    11701 accagtttga atacgattca agtgccgata ccttcagacg aagaagtaga acttgcaagt
    11761 acgaatgaaa tcatccagca gttaaaagct aagggagcag atagcaacaa gattggttca
    11821 gtgatcgttg gctatacatg gcaaaatgtc aaagaaacga atcgtgtcgt tgatctctta
    11881 cctgaatggt atgtgaaata taaagataac tggtattcag ctaacgacct tctggaaaaa
    11941 ttacctggat tggaggcgaa ttaattggat ttcaaaaaaa tcgaatggat atttttcgtc
    12001 gcttttttag ggttgaatgt gtttttattc agcagttatc atgacgcggt ataccaagaa
    12061 aacaatgtca ttcgctctaa tcagactgaa tcaatccaac aacgtctggc tagcgacgat
    12121 atcacttaca cggggaagat ttcgtctgag aacaagcaag gctattatct tagcgcggag
    12181 cagaccaatc ttagcacttc attagcgaat tatcgcaagg ctggtaaaag cggcttgctg
    12241 gcaagtggtg tgtttttaga cggtgatgtt ttgacgaaat ctttatccag ttcagatgct
    12301 gaaaagaacg tgatcgatcc agataaaatt agtagttcct tggaagattt tttatctaac
    12361 aaagacaata ttttattcgg taatgattat gtgtacacga aaaacttttc tcatttagaa
    12421 gaagaaccac tagaaatcca agcaagccaa gagtatgaag gcatcccatt caatgatgat
    12481 acagcaaaaa tcgttctttc tttagaaaaa aacgataatg attatagcat cacgaaatac
    12541 atgcagacgc atttatctga tatcgagcag ctgcgcgaaa agacggaact gtatacggaa
    12601 gaggaagcaa tcaatacatt gtacatcaat aacaaaatac caagaggatc aaaaatttta
    12661 tggcgtcagc tagcttattc ctgtattctg aaagtccggg aaaaaaatgt gtatgttcct
    12721 gtttggcatg tagcgatcga aacaagcgat aaagtggtcc aaattgagag cgtcaacgct
    12781 ttcagcaata cgatcgtgac aaacaatact gtaccaaaag tggaagatca ataaaaaagc
    12841 gagtataatg aaaaaagttt acaaagtaag aagggaagta ggagtcaaat ggctgaagaa
    12901 aaaccagctt ttaaaatcag tatcctagca agtggaagtt cgggtaattc actttatatc
    12961 gaaagtgaaa aaaaacggct tttggtcgat gcaggactta gcggtaaaaa aatcacttcc
    13021 ttgatggctc aaattgatcg gacaccggac caattagatg gaatacttgt gacgcatgaa
    13081 catcgagatc atatacatgg cgtgggtgtc ttggctcgta agtatcattt ggatgttttt
    13141 gccaatcagc agacttggga agcgatggaa ccactgattg gaaatgtacc agtggaacaa
    13201 aaacatctat ttgagatggg gaaagtcaga acattcggcg atatcgatat tgagagtttc
    13261 ggcgtctccc atgacgcagc ggctccgcaa ttttatcgtt tccataaaga aaatcggtca
    13321 ttcgtcatgt tgacggacac cggatattgc agtgattata tgcgaggtgt cattgaaaat
    13381 gccgatggtt atttgatgga aagtaatcat gatcttgaga tgttacgcat gggaccttat
    13441 ccgtggaatt taaaacagcg tattttagga gatagaggac atctttccaa cgaagacggt
    13501 gcacttgtca tgactgatgt gatcggtaat cggaccaaac gaatctatct tggccatctt
    13561 agcaaggaaa acaatatgaa agaacttgct catttaacaa tggaaaatgt tttgaaagaa
    13621 aaagattttg gcgttggaca tgattttgaa atctatgata cagatccaga tgaagcaact
    13681 gagctatttt cgatttaaac agttcaatca ataaaaatat aaaaagcaga agataaaatc
    13741 aatcagggtt ggttttatct tctattttta tttttttagc atattaaaaa aataaccgta
    13801 ttatcgactg actacactga tgtgctgctt ttcatgtttc tcatccagta tctcatctac
    13861 taatgcaatc gcgtaatctg ccatgctgat cgtactgtcg ctagcagcgt ttctttgtaa
    13921 ataatcatca cttaacaaat aagtacctgt acgtttgcca tgtggatcaa ataaagaagc
    13981 tggactgata taagtccaat gtagattttt agattgtttc aattttaaca atccttcgta
    14041 catattgaaa gctgttggat agaagggtgc atttttaggt acttggtcaa tcatacgagt
    14101 cgtcttttct gtatctaaga ataacgaaga ggcaccccca acgacaatta agcgtgtatc
    14161 ggttccttca agcatctcaa tcaaatggga caaagaagtt tgatgcattt cttcttttcc
    14221 tttgggagcg ttgaaagcat caacgacaac atcaaaagat gcaagatcag tagttgtcaa
    14281 ctcaaataaa tccttttgta taaacggaac atctgatggc aatttttctg gatgacgcac
    14341 gattgccgtc acgtctaaat ctctggacaa tgcttcgtct aaaataaatg aacctgcatg
    14401 gccagtagct ccaatgagtg caattttcat aaaaaacctc cttttttatt cctgtccatt
    14461 gtaagcagtt gctgaatatt cgtcaaagaa tccgattttt gaaaagctca gtgcgcattc
    14521 ctttcatggt tcgtgatcat tgggtatagt taaattaaga aaactgattg ataaaaatag
    14581 aagttgaagg agataagaaa tgaaagagtg gttagatcgt gcctttttac caatgataac
    14641 atctgttgtg aatcttagac ccttaactgc tttgcgtaat ggaatgactt acacgatccc
    14701 aataattatt gttggctcgc tgtttttctt atttgctcat tttcctgtgg attacatcgc
    14761 tcagtggata gacaaaacag gattttctat gtattttaca caagtatacc aggctacatt
    14821 tgatgtgata gccttttgga tcgtttttgc cattgcatac gcttatgcaa aagaagaaga
    14881 catcgatcgc ttaccagtcg gcataacagc cttgtctgct tatctactgc ttattcagcc
    14941 actaaatact cagatagata ctgtctggac aggtgctgaa gggatactag gagcagttac
    15001 aactgggctc atcatcggat ggggctatgc acgtttagta agacttgcga aagggtggaa
    15061 gcaaagagag aagatacctg aaaatgtttg gcagtctttt acttcattga ttccaatcat
    15121 tgttatagtc acttttacgc ttatcggttc agcggttttt caacatttgt tcagaatgac
    15181 tgttgttcaa ggagtatgga catatgtcca gcttcccatt caatcgttca tagacacgtt
    15241 gtttggtgtg cttttattag gtttttttgt tccgttttta tggttatttg gtcttcatgg
    15301 atctgcgatg gtcaatgggt tggttagtcc cgtcttgcaa gcgaattcat tagcaaatgc
    15361 agaaatctta gcctcaggac aagagttgac ggtggcaaat ggcggacata tcgttactca
    15421 gcaatttctc gatcagttca tgacggttac gggtgctgga ttgacgttag gggctgtatt
    15481 ttttatgacg gtttttgcga aatcgaagaa atacagagaa ttaggaaatc ttgctctttt
    15541 accagccttt ttcaacatca atgaatcaat tattttttcc acaccgatcg tgatgaaccc
    15601 gatgatggct gttcctttta ttttcgcacc ggtcgtttca ggattgatta cctattccgc
    15661 tttatatttt ggattcgttc ctttatttac agcagtccaa gtcccttgga caacaccgcc
    15721 gatcctctcg ggtttcttag taggcggagt gcctgcagcg attttacaag gtatcgtgtt
    15781 gtcaattagt tttttcattt atttcccatt tttgaaaaaa atagattctg caaactgcaa
    15841 aaaggaaaga cagacaaaaa atgatggagc agcagaaaag atgaacgact aaaaaacaaa
    15901 aatactcctg tcgctttaga ttgggaaaat caccaatcta ggatgacagg agtatttata
    15961 gtcatgttta tactagccct ttttggacag ctaattgaac aagaacaact aatgtgttca
    16021 ttcctacgtg tgtgatcatc gatgtccaga tccgaccagt tgctttgtac tctaggctga
    16081 agaagaaacc taggaaaaag tagattagca gatgaccatc attatgagca aacgcaaaca
    16141 gcaaagagct gatcactgct ccgacccaaa aatttgaata tcgactgatg atccccagta
    16201 tactgcggcg gaatacgaac tcttccatga tgggtccagc aacagttgtc gccaaaataa
    16261 aagcgggtgc ttccatgatc atctggatga tattttgtgt attctcggaa ggggttgctt
    16321 tgccaaacaa tacagattct aacatgacag cgatgctttg cagaatcaat gcgacaaaga
    16381 ttccaaccaa tccataaata acaatctttg gagcagaagc tttttttacc gctgattcga
    16441 tcgctaaggg ttcacgttgc ttaaaataaa aaatagctag gacaacggcg cctagtatat
    16501 acatgactgc agttgctgtg atcaattcag acgtagattt tacaagtcct atcgaagcta
    16561 ggatcaaggg tgagataaaa attagaccat agcaaaaaat gctgaggaag ctatattttt
    16621 ttaaagacat gtaattcctc cttttaaggg ataacaagca tgcaaggcaa gcttatatcg
    16681 attgatttca attatatcat aatttcaata agcagctgtc agaataaaat ttcttttctt
    16741 tgatttaaaa gcttgcaaaa aagagggaga atgagtatta ttaaaaatgt aggttagcac
    16801 tcgaaggtta agagtgctaa cagattaaaa aaagagttat cggagggatt tatcgtgtta
    16861 aaaccattag gtgatcgcgt cattatcgaa gtcgcaaaag aagaagaaaa aactgtcgga
    16921 ggaatcgttt tagcttctgc tgctaaagag aaaccccaaa ctgggacagt tgttgcagtc
    16981 ggtgaaggcc gtttgttaga aaacggtgaa aaagttcctg ccgctgtaaa agcaggcgac
    17041 caagttatgt tcgaaaaata cgccggtaca gaagtaaaat acgaaggaaa agaatatctg
    17101 atcgtcgcag gaaaagatat catggcgatt gttgagtaaa tttggaagat aaaaaaataa
    17161 tcaaaaataa tcaatgaggt gaatcaatca tggcaaaaga attgaaattt gcagaagacg
    17221 cacgcgcagc tatgttacgc ggggtagata aattagcaga tacagtgaaa gtaacattag
    17281 gacctaaagg ccgaaatgtc gtactagaaa aatcttacgg ctctccattg atcacaaatg
    17341 atggtgtgac gatcgcaaaa gaaatcgaat tagaagatca ttttgaaaac atgggtgcaa
    17401 aacttgtatc agaagtagca tcaaaaacaa atgatattgc aggagacggt acaacgacag
    17461 caactgttct gacacaagca atcgttcgcg aaggtttaaa aaacgtaact gcaggtgcta
    17521 atcctctagg cattcgtcgc gggatcgaat tggcaacaaa agcagcagtc gaagaattgc
    17581 ataatatctc aactgttgtt gattcaaaag aagccattgc acaagtagca gctgtttctt
    17641 cgggttctga taaagttgga catttgattg ctgatgcaat ggaaaaagtt ggaaacgacg
    17701 gtgtcatcac aatcgaagaa tcaaaaggga tcgaaacaga actagatgtt gtagaaggta
    17761 tgcagtttga tcgtggttac ttatcacaat atatggtaac agataacgac aaaatggaag
    17821 ctgttctaga aaacccatat atcttgatca cagacaaaaa aatctctaac atccaagata
    17881 ttctgccatt gttagaacaa atcttgcaac aatcacgtcc attgttgatt attgctgacg
    17941 atgtagatgg tgaagcattg ccaactcttg tattgaacaa aatccgtgga acattcaacg
    18001 tagtagctgt aaaagctcct ggtttcggcg atcgtcgtaa agcaatgctt gaagatatcg
    18061 ctattttgac aggtggtaca gtaattactg acgatctagg tttagaattg aaagatgcaa
    18121 caatcgaaaa cttaggaaat gcttctaaag ttgttgtaga taaagacaat acaacgatcg
    18181 tagaaggttc tggagaaaaa gaagcaatcg aagctcgtgt gcaattgatc aaaaaccaaa
    18241 ttgctgaaac aacttctgat tttgaccgcg aaaaattaca agaacgctta gctaaattag
    18301 caggtggtgt agctgttgtt aaagttggtg cagctactga aacagaattg aaagaattaa
    18361 aattacgcat tgaagatgca ttgaatgcta cacgtgccgc agtagaagaa ggaatggtct
    18421 caggtggtgg tacagctcta gtgaacgtta tcagcaaagt ttctgctgta gaagcagaag
    18481 gagacgtggc aacaggtatc aagatcgttg ttcgtgcttt agaagaacca attcgtcaaa
    18541 tcgctgaaaa cgctggttat gaaggatcag tgattgttga taaattgaag aacgtggaac
    18601 taggcactgg tttcaatgct gctactggag aatgggtaaa catggttgaa gcaggtatcg
    18661 tagacccaac taaagtaaca cgttctgcct tgcaaaatgc cgcttccgtt tctgccttgt
    18721 tattaacgac agaagcagtc gttgcagata aaccagaacc agcagcgcca gcagctcctg
    18781 caatggatcc atcaatgatg ggcggtatga tgtaagaaaa agcaacttgc ttatctacaa
    18841 ctgatcatta gtgaaagaca gataaaaaga ggctggaaca gaagcgttta actccaagaa
    18901 ataagaagaa attcacggaa attgcttttc aaatttttgt gaatttcagc ttatttccga
    18961 aggagttgct tctgcttccg ctgtttatac gtgtttagag cgtgagacaa aagtgttctt
    19021 tactttt
//
LOCUS       DAZKMK010000054        18618 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000054 DAZKMK010000000
VERSION     DAZKMK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.054"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..915)
                     /locus_tag="ACG8V1_001767"
     CDS             complement(4..915)
                     /locus_tag="ACG8V1_001767"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0329861.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1292..1930)
                     /locus_tag="ACG8V1_001768"
     CDS             complement(1292..1930)
                     /locus_tag="ACG8V1_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0329862.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1934..3916)
                     /locus_tag="ACG8V1_001769"
                     /pseudo
     CDS             complement(1934..3916)
                     /locus_tag="ACG8V1_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4096..4761
                     /locus_tag="ACG8V1_001770"
     CDS             4096..4761
                     /locus_tag="ACG8V1_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329863.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5158..7191)
                     /gene="brxL"
                     /locus_tag="ACG8V1_001771"
     CDS             complement(5158..7191)
                     /gene="brxL"
                     /locus_tag="ACG8V1_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0329864.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7309..9831)
                     /gene="pglZ"
                     /locus_tag="ACG8V1_001772"
     CDS             complement(7309..9831)
                     /gene="pglZ"
                     /locus_tag="ACG8V1_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0329865.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9947..13579)
                     /gene="pglX"
                     /locus_tag="ACG8V1_001773"
     CDS             complement(9947..13579)
                     /gene="pglX"
                     /locus_tag="ACG8V1_001773"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0329866.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13643..17314)
                     /gene="brxC"
                     /locus_tag="ACG8V1_001774"
     CDS             complement(13643..17314)
                     /gene="brxC"
                     /locus_tag="ACG8V1_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0329867.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17339..17914)
                     /locus_tag="ACG8V1_001775"
     CDS             complement(17339..17914)
                     /locus_tag="ACG8V1_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0329868.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17914..18513)
                     /locus_tag="ACG8V1_001776"
     CDS             complement(17914..18513)
                     /locus_tag="ACG8V1_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0329869.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5736 a         3560 c         2609 g         6713 t
ORIGIN      
        1 cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa cattttcaaa
       61 gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta ctttattctg
      121 gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt
      181 atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat attccgtagt
      241 ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt tcaacttaaa
      301 ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc
      361 gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag agaaatcgtc
      421 tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta cttggataat
      481 ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt catcggtcaa
      541 tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac tgtctttgaa
      601 tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac ccaattgttt
      661 ctttgtttga tcatattggg aatcgatttc tttggttgat acgtcatctc catatttatc
      721 ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt gcattttttt
      781 caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa cggctgtttt
      841 ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg caagtgctaa
      901 aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt gctaaaaaca
      961 aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga tacgtcctga
     1021 atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt caagtagcac
     1081 atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct tgcgttccgt
     1141 atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt cttccgctgt
     1201 tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt tgttgttttt
     1261 attagaaaaa gtttgttttt tcatttgttt tctaaagcat gatcttcttg tttcctattt
     1321 tggtcagata tggatcatgc gtgaccacga taactaacgt accttgggaa gcctgttgcg
     1381 ataagatttc aacgacttgc tccttatttc gtttatctaa tgatccagtt ggttcatcgg
     1441 ctaaaatgat tttgctattc ttcaataaga ctctcgcaca ggcgattctt tgcttctcac
     1501 caccagataa tgtgtagata ggcgtactta gcttcttgtt tatatttact tttttcaatg
     1561 cctcatactt tgctgttcgc ttatctgaaa catttaaatt cgaaaacttc aatccaatat
     1621 ctaaattaaa atcgattgtt tcatattcaa tcaacccaaa atcttgaaaa agaaaagaga
     1681 gggcatttct ttttatcaag cgcttctctc ttccagttaa atgttttgtt tcttgattaa
     1741 ataaataaaa ttgccctgaa ctgaattgat cattcaatcc taatatattc aataaagtac
     1801 ttttccctgc tccgctctct ccaactacag tcacaaaatc cccagcatcc gcagtgaaac
     1861 gatccaaatg aagaatcgtt ttgtttcgaa tgattttttt cgcacctttt atcactatca
     1921 acggctccaa ttgttaacct ccattcatca gcaataactg acttttcttt tctgtagaca
     1981 aaataaaaac aactgaaaga ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat
     2041 atttaggctc tctttctata agaaaaagga agattacact tgtcgttccc catttgatga
     2101 ggttcatcat gaagaaatct ttatacgttt caaagagcga ataaccactt gaacgaaaga
     2161 tagcgatttt ccgacaattg tagacaaaat atatgcataa catgacatag ctcaacaaga
     2221 agaaagaaaa gaaagtgagt aaaaatatct gtatcaagta atgaaatgat ctgcttaaca
     2281 ctttgacagc tatctcttca gtctgagtat acggaaatga taactgcaag ttatcacgta
     2341 actgactttc ttcaatcagc tcaaataacc tttctttcga caaagagcct atcttgatct
     2401 ttaacggtgg atagatttct ccgctgagaa tatttctttc ccaatacgta ctgtttttca
     2461 atgtcagtat ttcaacaacc ggataatgtt cgatccaggg gatagaagga ataaatgtat
     2521 aaatgggttg attgtttttt ataaatagaa atttcggttc tttttcactg atttcttgat
     2581 aatattctgt tatttgcgcc aggtcatttt ttaaatctgt atgtctatat cgttcaggga
     2641 tcaatataac agggtgcgtt tctttttcgg ttatttggat caaccttcca gagacatcaa
     2701 tcaaaggaaa tttcttaaga taattcagat ttatttttat ccctctgcca aactgttcat
     2761 tttcttcatt caagtaatct gatacattga ctaatatact tccgttttta ttcaaagttt
     2821 catataattc ctgatcggct gctgatgcaa aacgattgga ataaataaaa tccacgtggt
     2881 ttttcccgat ggttactgga taaaatacat gatagtcctc accagcgatt tcttttggtg
     2941 tttcatggga aacagctaac tgtgaagaag aaaaaacgat agttgctaat tcgacattgg
     3001 caacggtcaa aagaagagaa aacaacaatc caaatgtaaa aagaatgtat tggatatagt
     3061 tgaacgcttc tcttaacgaa gcgatttttt caactaataa aagagaaatc aaagagatac
     3121 cctatattcc gctgaacaag aaaacgtaca tctggaacaa ataaagaaat tgctccttat
     3181 aatacataaa aataaaacca ccaatgattc ctgcaattat gaatgtaata atgattttct
     3241 ttttccccaa taaacaccac aatacttgat gtaatgaaaa gccattattc aacaataaac
     3301 tgatttcttt attcatcaat aagaaaaagg atgataacaa aaggaataaa agaatcaatc
     3361 caatagttaa atacactcga taattatata cgtctgtact tgaaataacc gaatacgaat
     3421 agctattttc aaagtcttca aaaatattag tggcataatc agtgtgaaat gcttgattga
     3481 atcgttccac aaatagtaaa atgaaccgtt cataggcttc tttttctctc gtcatcaaga
     3541 aaaattcacc ttcaaaatct tttattttga tcgattcttc aatggacgtt acgcgaaagg
     3601 aggtattaga atagggtacc ttctcgtgag aagatttgac atccccttga taatacaatg
     3661 tcatttcaag ctttggctgt atcttgaagt tccaagatgt atctacttca ttggattcat
     3721 ttactgtttt tttcatataa gaaagctcag tttctgatga gacattttca aataccatga
     3781 ttagctgctc atattcttct tgcccaataa aatgttgtgg caatcgaatc ggagtataag
     3841 tatctgtttg atggaaaaat tcatttgtcc gatagagtac ctgtttattt ataataacca
     3901 aggcaaaacc actcatgata ccggtcacta tgattgtgag taaaactgtg aacttttttt
     3961 tgattatcac cactctccct tacttaaatg attctttgat aaaatcatac cgtttttcat
     4021 caaaaaaacg tatctatcaa atgtataggt catgtagaat aaatctatat actgtagaat
     4081 cgaaaggaga agaagatgaa catcggagaa aaaatacgaa cttaccgcag aaattgtaag
     4141 cttacgcaaa aagaattagg cgaaaaaatc ggtgtttccg ataaaacaat ctctagttgg
     4201 gaaaattctc gtacaatgcc agaccttgaa atgctgtcac ttttacatca gactttaggt
     4261 ctgccgattg attttccttc tattgaatca gctaccgaat caatagaacc aacattgccg
     4321 gatgaacctc ttgttatcaa gaaatcttat tcatcaaaac aacccttctc actcaaaaag
     4381 acactatctt tctttctgct cgtcttttgt ttggtctatc tttttcttat caattatcaa
     4441 tataaaaatg aaacggttga tcgattcaat ccttttttga aaccacaaat cggctatacc
     4501 acactccctg taggatacgt ggaaaattcc atcgaacatt gggtatgtaa tgatatcttt
     4561 gttcatggta catggctgtc ttttcacgct ggagaggtag aaaaaggtaa gaatcatgct
     4621 gtaatcaaac acaaaggaaa acacgtcatg aacgttcgcc tgctcacttg ggaagccatt
     4681 ccctcagatt accaagaaat cctcatccaa gactatgacg atgccttaaa aagaagccct
     4741 caggagccag ataggcatta agtaggagat gaacgtagag atagaaaaaa tcaatgcaca
     4801 tgatcttatc aaaaaaaaac aggggtacaa attaattgaa aaagctttga gatgaaagtt
     4861 cctaaataaa aaagttcaga atgataggta aaaaaatgac taactattct agtattaggc
     4921 tatttgtatt attcttctgt tatttttata tcaaaaaatc cagaaataac taaatatagg
     4981 tatttctggc ttatagtata atatttacgc cacagccccc aatgattctt tttggaatca
     5041 tgatcaagga cggacataca caaaaatatt ttgcccattc gatccgaata gattaacgac
     5101 taatctagta agtaatatag attggtacgt agtcaaacca gcaaatggaa aagttgtcta
     5161 ttccacccca agggctttaa agacagcatc ttccggcgtt tgataaaaaa tcaaattaaa
     5221 ggtacccatt aattctgggg gtacagttcc aatatcagca gcgcttatca taggtacaag
     5281 tacttttttc gctccggcat cttttgctac ttgtaaacag ttagctaaat tatctacctt
     5341 aatcatagaa ccaccaattg taatatcccc taaaataact aaacttggta atacaggttt
     5401 atttaaggaa actgagcata aagctattat agttgataac gataatgttt ttgtcatacc
     5461 aatgccattc atatcttgaa catgaacgac aaaatctttt gtcgtcaaac taatagactg
     5521 actaacactt ccataattag ctcttaaata tcggtatgcc gaatccatcg cttctcttgc
     5581 tccagcatct gaattgatcc cagtacgttc aaactttcca tttcccgaag tcatttgcgt
     5641 ttccaattta taggtaccta attgcccagt tttgccataa gataccgtgt acaaatgccc
     5701 tggcttccct aacccttctg gaatcaaagt tcctccacct tgttcaggaa cagaaacata
     5761 ctcttctcgc tcttcttcta aatcaatgta tgaaaaattg acatcataaa attccattcc
     5821 accaattttc tttaattgtt cttttacacg acgacgcatt tcaagagcaa atcttagtat
     5881 ttcttcgaca tcactttttt caaatctacc atcaggatac aacagtttca tcatcccaga
     5941 tacaatccga cgaactgcaa tcgtatcccg ctgatttaat tgagaaccaa gtttgaaata
     6001 tttatcaaaa ctatcgccaa aactaacttt tcgaagctca cgcatgactt cagaaaaata
     6061 atctgtaatg aagccatagt catttgtaaa aaaatctggt cgatatttag gaatttccca
     6121 acctggtaaa taacaatgca tccgatccaa aaaggctgaa tccgtcccca taacctccgg
     6181 aaatggctca aataaattcg aagttttcaa tattacatca acgctttgat taatatttcc
     6241 tacaaaagcc attgatgctg aagcattctt ttcttctttg cctctagcaa aagatcctga
     6301 agccatgtaa tctttcatga tttgaacgcc atcattatcc ttaaatttaa tcccagcaac
     6361 ttcgtcaaaa gcaacacagt cccacatacc tacaagtccc attgtacgac tagccatatt
     6421 atagaaaagg tttgctactg ttgtttgtcc acctgaaaca aggattgagt ttggtgaaat
     6481 ttctttatat acatgcgatt tacctgtacc acgtggtcct agttcacaga gattaaaatt
     6541 attttcaatt aatggaataa gtcttgctaa atgaagccat tttacccttt tattaaattg
     6601 tgtaggttcc ataccaatgg accgtagaag taagtcaatc cattcatttg tagtaaactc
     6661 cttgcgagcc atcttgtaag cctcaatgtc taaaccaggc atttggattg gatcaacctt
     6721 ttttgcaata aaagggctat tccaacgatc attctcatca taccaatatt ccatcgaaac
     6781 aatacaccag atccccccac ctaataatcg atcaaatttt ttcggatact cttcactaat
     6841 ttgaacattt tttaatccca ggttagagaa ttctgcttca taacaatctt tcttttcatt
     6901 taaatgtaca gttactttat caataactgt ataaccaccc aattcacgaa tttttgcttt
     6961 tactttttct gcttcatcag gacgcacata gtttcgagca agaatatttt taacattttc
     7021 cacacctttt tcaaccaatg tttcatccaa tgacgaagca tacataccaa gtaaatattc
     7081 caatacataa acaggtacat tcgcaccttc tttaattttc tttgttaaat cttttctgac
     7141 aatctttccg ggaaagaatt cagataactt taaaatttcc tgttgttcca ctagaattcc
     7201 tccatgtgtt cagtcattat aatagcaatt aaagtatatc atacttaacc agtaggcttg
     7261 ataatacaaa agaaaaagta tccacaaaaa attgtggata cctcgttttt atatatcaaa
     7321 atcaaatgat tctgcaaaag gattatctat aatgaatttt attcgctcgg tcaaatcatt
     7381 tttttgattt tttatcacta aataataagt atcttcaact gtatatcgac gatttacaaa
     7441 ttcaaaaaca aatttagtaa accgctgatt tgcatcttct gtttttgaat ccgcaacata
     7501 gatttcttca tttgagatac gttcaccata ttcatcttca aaataaagcc agaaagatgc
     7561 ggggtcataa acatcactaa ttgcattttt ttgatagaat tctaacgtgg ttgataatcc
     7621 tgtaattctg cggttgctag tcattaattc aattgctact ggttcctttg cattgctacc
     7681 tttggctacc gtaatttcta gaacagggac tagcatttct tgaatagatg accctccatg
     7741 aacgtagctt tgtcccccgc cagctttctt aaaaatactt gaagtaatcg ggacacaaat
     7801 gtttctagtg tcatcatttc ctaaagaatc tcccaaagag taatttttcg taccaattat
     7861 ttcgtattct tcttcactaa gaataaaacg acgttctaca cgatcttttt catttctaga
     7921 tggattttca atcttatcta cttcttcaac gtggcttctt ttataaataa atccatgatc
     7981 agccgtaact ataaaacgat agatactgcc atcattatgc aagcgattaa taagtcggga
     8041 aatttcgctt atcgctaaat cacaggcctc aaaaactcga ttttcattcg agtgatcacc
     8101 tgttctatcc acttgatcgt gataaatata aatgactgat ttgccattaa atacttctcg
     8161 tacctcagcc ttattcatct taaccacatc atcataattt aaagctacag catttgcatc
     8221 tcgttgctgg agaatcgcat ttcgattgga taaaccattc gtttttttcc catcaatcag
     8281 tacttctgtt ccttcattgt aagttagttg attgtttggt aataaggcgg ctttaccgaa
     8341 ttcagtaact gaaggtaata cagaaagaaa gctagccatg tttgttgaat acttcttttc
     8401 tctaactaac ttacgttcta aattcatccc taactcatat cgaaaagcat ctgaaataat
     8461 aactacagtt ttaacagagc gtttcattac agcttgatta taaaattttc gaatggatgg
     8521 acgattctta aattcaaaag tttgattcca aatttgtcct gattgatcaa gaaacttcga
     8581 ataatttgct tcaactaatc gatacaattg gccatatttt tgcttttctt ctatatgatc
     8641 actattccaa ataaatttcc gataatgcgt atcaacccaa tacatttcct tctcatattg
     8701 agttagcaaa ttatccatgt tcatcggcaa gcacaatctg tttttatcca caagataata
     8761 agcatgtttc aataattgat actcataaat ataatccgat gcgaaatgca ttttttcgcg
     8821 ctgctttaaa ctatcaaaaa ttgtttgtcc tgcaattttc gttgtagtgt cagaagcctt
     8881 taatcgttct gcataaaaca ttaatatttt tgcatgaatc tcagaaaata cgtctgcttc
     8941 aattagatca tcaatttcta tactgtctaa taatccatca ccatcaatag cctgatatac
     9001 ttttttagat agctgttcaa agctttccat gtaacgtgta tcattcataa actgatccat
     9061 gaaagtaata atgtttgctt tcaaatgtag tatttcaaat cctttgaact tcttcggcat
     9121 ctcttgatca atttgataat aaaaatagtt caaataaaga cacatcgtca gcttttccaa
     9181 tgttggctgg ctatccgtaa agccaaattt catttgaata aattcccaaa atttagctgt
     9241 taactgatac ttatcaagtt cccttaaagg attctcccta ctttctgtaa agctttttag
     9301 aactacacgc agtaaatcat ttaatcgaat acttttcgat ttcaacaaaa cagccattat
     9361 cattaattca atcgtttctg atttattaat tgtcatatgc gtactaaatt caccaaaact
     9421 tctcatgcga tcctttgcat taaagaaatt acgatactgt ttaactgtct gatgaaatct
     9481 aagtggaata gccaattcat tcatgataat cgctaagcgg tctgcactaa attcctctga
     9541 ataattcaat acactcaaca aatgattatc ttcatcttta tcatcccaac gaggaaatgg
     9601 cgcatagatt aaatagttag tttcgggatc ttcatattcc aataaacgct tagttttaaa
     9661 ttgtgcgtcc ttttctaatc gatagatttt tgcattttga atgaccaatg agtcaatttc
     9721 ttcaagaaaa tcctgatttg aatcaaacca aaaaatgatt ttcctacatc cataattagg
     9781 aaaacgttga tttaattttt cttcaatttg ttgcgtattt atatctgtca tcaatctcac
     9841 ctaatcgttc tctttctata ccccttatat tgacataatt tggataattt cgcacgcaat
     9901 ttaagaataa aaaagaaagt tgttagaaaa caactttctt agataactat ttttttggtt
     9961 ctttgacccc ttttgcaaaa atttggaatt tctcgccggt tttaggatca cgctgcaact
    10021 tatcataatt gacaattaca ccatcatcta agtctaattc aattcgtttt aatgctaagt
    10081 gacccaactt ctcgtcatac tcacgaactt cttttaattg cttctgaatc tttacaattt
    10141 cttttgtcat tgcatttctt tcttttgggt tatcacttgt tgaaatttgt tgctgacgca
    10201 attcgattcg attgtcatac gctttttgta gttcgtgtag gtattcagta cgtacttttc
    10261 ctaattgatc tggtgtatag cgatgcaagt acattaacgc tttaaagcca ttttgttttc
    10321 ctgagtccaa ttgccaataa attggtcgat tggagtatgt ttttaaatgg tctttgtaaa
    10381 aatcttgtac gaagtaacga cgaataattt ctcgacttgt ccctttgcca cctaaagtgt
    10441 cagcgataaa ctggagattt tcttcaaggg tttctttacc aaaagtaatc tcaacgaaat
    10501 caatgaaacg attgacaatg tcaatttggc tatccttgaa atactcatca tcggtgatga
    10561 gtaaaacatt atcagtatca ggtttaaatt tttgatagcg ggaaggatcc cattcgccgc
    10621 cagcgaagat gagaccatct tcgtctaggg agtaacgacc gaagatacaa cctattgaat
    10681 aagaaataaa atccttaatt tccctcgcct tatcaggtaa ccttgcagag atatattcat
    10741 cttgaacctc ttttgttaat tctttttcaa gagaataatg attaatgaat atgtcgttca
    10801 gttcctcttc attcgatttt agagtattaa attgattaac caactgccta cttagacagt
    10861 taaatgcctc tgagagatct tttttaaaca tataacttaa caaaatgttt cttctaaact
    10921 cgtatgacgt ttcaagagca ttatagtctt ttctagctat atcgatattt tcatccactt
    10981 tttctcttat tttttttaat tttctctcag atgtaacttt aagaaaaggt agttttccca
    11041 ttggtccttc atggtaatcc aaggtgggag aaataaaagt taaaaagtga ttaactaatt
    11101 tagagtttac atagccaaat atttcttcaa agtcagttat atcctttgag aacagaacag
    11161 atcctttagt ttcaaaaatt gcctgattct gcaatctaaa agatacttgt ccagatgaaa
    11221 gtgtcgacca agtaattccc tttttaaaat agtaattaag attttgtggt ctagatttta
    11281 acttcccatt ttcattaaca taatttttta tttcatctcc atcattctcc cagttaacca
    11341 cgtattcatt gtttccatac cattttctaa aacgaccacc tttagttgta ggaaaccatt
    11401 tttttctaga ctttttagcg tcctctaatg ttttagcatc aaactttatt ttgttaaatt
    11461 ctacttcaaa ccaataccgc aaaaatttat tgttatttcc ggttgccaac ccttgttttg
    11521 catctgcaac atcgttagtt aattttcctt gagcaaaatc agtgattacg ttatcgcttg
    11581 cccagtaagc aatcggcatt cctgggattt ttttgaagtt gtcttgatta gtactataaa
    11641 aataatcgca ttcaccatgc tgatgaattg cttctaacgt tttaattttt tgaacttcca
    11701 ttccaccttt aaaatctgat aattttatat agcttccatc ttgttcattt gaatttttta
    11761 tcgtaaacgt acaaattgga acggttgctt cttcaaaagc tgaatactcc atttgaatca
    11821 aactagaaat gtttctttca ttaattaaaa atgagcgtaa cttttcatag ctagaaatga
    11881 acatccaaac aaatggcgtc ataaaggaag catatccatt atcaatcagc atttggctat
    11941 tcaatttaat aaacacagaa aatagatctt ttttgacatc agaatagtta tcatttacat
    12001 atttttttaa tttcttatcc attttattaa gataaggcgg attagtgact accgctacat
    12061 atttatcaac tagcatttca gccacgtcaa gaatgtgcaa gtatttttct tgtatttcct
    12121 tcatcccata aagaatatcc atattcgtaa attcgttgaa ttttgatata tactctctta
    12181 cttttcctaa gtattcttta gttattgcct gattattttt atcgactaac ttaatgattg
    12241 atccaagttc tttcccattt tcaaattgat caaaaattaa ctctttgtat tcattccagc
    12301 gttcactata cttatcattg ttcaattcac taatacgctc ttgatactcg tctgtcactt
    12361 taggagaatc aataaactct cttaaattcg gcgatagact ttttttcaaa aaaagacgat
    12421 cgtattgacg ccctttcatc ataattgcaa aataagacaa ttgatacgct cttttatcga
    12481 tttcaagacc atataaattg tattcaacga ttgcacgtgc agcatcacgc tcagaatatc
    12541 ctaacgtaag ataaatttcc atcaatacat cgaaggcgta aactaaaata tgcccagacc
    12601 ccattgcgtt atcgataatt tttagttctt ctggttttaa attacttaca gatgttgctt
    12661 gttcaaaagc atatttatct ggaattaagt atttcaactt atttcttaat tcactattgg
    12721 gataatgatc taaccaatac tttcctaaac tattgtcaac catataacga acgacccaat
    12781 ctgtggtaaa tagttgagtt gctgccggaa tatcttgttt tttgacaatc cctttataaa
    12841 tattcaccac ttgattatgt ggctcactat tatagtactg atacaaccac ccaataatct
    12901 ctacctgtcc accagcttga acatagaaat catcctctgg aatacttaaa agatcagcga
    12961 ttacaccatc ttttttaata taagaagctg taaacaaaag ttctgtataa tcttctactt
    13021 cttcaaatag ctcaggtaag taatcattca attgattaca gatttttata aaaatcaatt
    13081 gatacagctt gtccattgct tctgctgaat tatctgtttt ccaagttctg acttgttgct
    13141 gttcattact agataattct tcaaagatat cggcatcata aatatctgta ataatatctg
    13201 gctcaatttt cccacttatt tcactagata atacacgata cgtgtcaggt aaataatcat
    13261 ttacctccat aaaccgaatg gcaatcaatc ggttaaacca cgtataagca acctcttcga
    13321 ttaactcttg aaaagcttct gaataagaag tgacagaagc acgttgtttt agttctgcaa
    13381 tcaatttgtt tctttgcgct atttcttttc cttgaattct tattcctgat ttttcgattt
    13441 cttgtaaatt gctatcaatt ttcttcacat cttcaatttt tgattcatca attcctaaac
    13501 gttttgcttt agtcattact tcatcaatta attttctacg ggcattgacc gcgaaattct
    13561 ttatcgcttt cttatccatt tttctttccc ttctatatgt aacctttgaa aagacaggca
    13621 cctttttatg cctgtctttt cactagaatt gaatcgtgat ttctgtattt tcttgaagtt
    13681 gttccttcag tttttgatta atttcagctg ttaactcatc aatatctgct gctgacttaa
    13741 tagttttaac atgaacgtta agtgttctca ttggtactgt tttctgttga atagtaggtt
    13801 ttactatcgc tatttcttca gcttttgctg cagagtctat ttcatctttt tcagtttcac
    13861 gaatcaagtt tgctgcttca atttgtttac gtgcttgttc ttcttgacgt ctgcggtttt
    13921 cttcattatc gaaacgttga ttaaagatca aatactgttc tgctacgttt aatgataaga
    13981 aaacaagatc atctatagta ttcgcttgtt caatttttgc tttcagttct tgaaattctt
    14041 ggtcaactat tggtacaaat tgatccttaa aatcttttcc ttctaataat ttaaatgttg
    14101 cttgtttttc ttccgttaat ttatttaata tttcctgact ttccaactct aacagttgat
    14161 tatattgttc aataaattct tgaatcaact catttagttt catcatatct gaataaggat
    14221 cagccttttt caaaatggtt ttcatctcat gaatcaattg atcaatttct tggctagaag
    14281 tttgacgccg acttttttcg attaatctaa ttttatcttg tgcattctcc cagatttttt
    14341 tctgagtact attttgcttg tggaaatttt taattttatc atatgtttca gcccagtcga
    14401 ataattcatc gcgatatttt ttgattgtat aaaaaaagtc ttcaacatct gacgtttgaa
    14461 caattacttc aagatatttt tttatttgct catattcata agcactaggg taggtataag
    14521 tgttttgtaa atcaaaataa gagcgtaaat tgtctaattg accttttaaa tatttcttga
    14581 aattttccat tgtatgttca ttgttatttt catcaacaat attacgactt tgaaacagtt
    14641 ctctaccgat ttcgtcaacc aatttttttg cttttggcga aatcaccgcg cttggttcaa
    14701 tttttatctt ttcactttct cgtcgttttt gaataatcat tacaatacta tttggatcat
    14761 ccatccgtga aatttgtaca ttgttataaa gtaaatcaat tttaccagac atgaaaagtt
    14821 tagcgatgca ccattccaca tcaagttctg tgaatccata cggttgtttt gtttcaaaac
    14881 gatcacgaat tgttttcatc gtaatcgtta ggtgatttct cttttgatca ttgataaaat
    14941 tgaaaatttc ctgaactgct tgctcatttg atggatcttc aataatttca tttttgcttt
    15001 ctttcactaa atataagata tcttcttttt ctttaggcgc atcaatttgg tttaatttat
    15061 ggtatacttc ttcagccatt ttctgaagtg ctgattggaa ttttgcttta aagtcggagc
    15121 ctgtcagttg aatttctgca ccgttataga aaaattttgc agatttcaac agttcattta
    15181 attgatcgtt gatacttgca cttagttcct gtaattcacc agatttttcc gaacgaatcg
    15241 cgtcaaagtt ctttactaca ctcgaataag tcgttgttaa ataaagattc attctagcat
    15301 acattgctat atcatcttta aattctttat ctgatggcaa ttctacgaat aataagttac
    15361 gggaactctt aagcgcaagt tctgcctctt gacctgctaa atcagagtat ggtgaaataa
    15421 aattaactgt catttcagca tttccagctt gcttgtatgg acgttgatct aacacttgtg
    15481 cataagtaaa cgtataccgg tttttaaatt tatttccacc tgacatgcct aatttttcat
    15541 aggcaaaact atttcggtct agaacgccgt caaaaattcg tttagatagt tctacgatca
    15601 aatcactatc tgtaatattt tgatttttaa tctcacgagt aatttcctgt tcttcatcag
    15661 tttggaaaat ataactatct aagttcttcc gcacaaagtt ttccttaatt aaaaggtcca
    15721 atgcttcttc tactcgtttt ttagtttcca ggcggtcttc gttgatattt cttatcatca
    15781 agctcgtaat attttcaaca tttggtttga tggcttgatc aacatattta atcatgaata
    15841 aaattcgtaa gacatttacg ttaaagttat ctatatcagt tggtctttga atatgagaat
    15901 tatttgtagc ttgtgtaatc actcggctaa ttgctgtatc aatccaacgt tctaacgagt
    15961 aataaaactg atctaaagaa accaaactgg ttgtatcatt ttccttattt cgttcagcgg
    16021 cttctttaaa cattgctagt aatgaacgtt caccagacga taagtttgat ccttgatagc
    16081 tgtgattacg aatctcggtt agaatttgcc ccaataaatt gaattgatag ggaataaatg
    16141 gataaacttc tgcgtaattt tcagcatctt ggaagagctt taattcagga ctatcaacaa
    16201 agagtaattt atttttcaat tctgtttgaa cagtttcaaa ttgtaaggct aaagaatcat
    16261 tggcaaactc ttttttcttc aaaatacgtt ctttaattac ttcatcggca tttgctgaag
    16321 ataaactcac agttgtggca aaacgatcct tgatcttaga aaaatcatta tcagaattgt
    16381 tatcaaagtt tgttacgtga tcaatcgctt cctgacttgt cactaatacc caagccttac
    16441 cttcacaaat tcgacccaaa tcttccacaa cggtttgtaa ctgcaaagtc aactcacgat
    16501 taccgccgat atattgtccg acctcatcga ctagaaaggc aatatgatgg tctgcttctt
    16561 tggaatcaag atagctttta actagatttg caaagtcatc aatgcttatt aaataatttc
    16621 ctgatgcttt ttctaaaaaa ttatcaatag cggcttcact cataaaatcg attttaagtg
    16681 ccgcttcttt gatatcatct tggatccaat caaactcatg gcgctcttct tgccattttg
    16741 ccccattaat ctcttcaaaa gcagctttga atgcatcaaa cttttcttct ttaattaacc
    16801 gacgttcaaa atctgctaga tgtggaatcg tgcttaatcc ttgcatttcg ttaaatacct
    16861 ttaaaaaaac attcacgatt ccgtttttgt cttttttacc acctgcatca ctttttgaat
    16921 caatattaaa cagaataaca tctgtattta tttgtgagca ccgttccatc gttgccaaag
    16981 tcagtggatc tttgattttc ccatcttctt tgaagtaatc aatggctttc ttgtcattta
    17041 tttctcgatt tgcgagcaag taagaaataa tttttaagaa atgagattta cctgaaccaa
    17101 agaacccctg aatccaaaca cccattttat ctgttggtgt attgatactc cgttggtagg
    17161 catcaaaaat tgttttaaag tgtttttgta gttctttcgt aaccacgtat tcttctaatt
    17221 cttgttgaac attttcatcc tttgcttgac cgaccttaat aactccttga atatttcggt
    17281 caattggttt tgcaaaaata tctttaatta gcatttaatc tcctaccttc catcccaact
    17341 aaattctaaa tgctcgataa taattatcat ctttattggt attaagaatt gtcaaactta
    17401 attcatcata ttcccctgga tacatcatca caactggtac gcgatcaaat acctgatgca
    17461 ttttatttaa aatagagtgc gctctaatta atggatataa ttcgccaatt cctgtcagga
    17521 acacaactga tttttctggt gtatgggcat ccacatattc aaaaaataca ttttcttcct
    17581 caggtaaatc cattttcaat aaacgaccaa tgctttccac taatttttgt aaaccacgtc
    17641 gttcttccat cttaatcgct ttttcagtga aaccgcgttt ttctagatac tctaacataa
    17701 tcacatagag attataaact ttcacttcat aattatcacc aacagatgtt ccagatatat
    17761 cattaatata atctcttaca attaactcgt ctttcggatc gtagtcaaag atataatagc
    17821 ctacttcatt acctaaacca cgattcaata aaaaatcttc tttttgaatt acttcattca
    17881 acttttccaa atgctcttga atcgataatg acattataaa attcctccca gtgctagtac
    17941 aatatcttta cgattcttgt caactaacca ttgaatcaat cgtttatcta ataatatcgg
    18001 agtcattcgt ccatattctt tttcccctct gactaatcct gtttcaatta gattcgtttg
    18061 ataagcggca ataagccgtt taatcgtata atctttccat tttgaaatat cttctgaact
    18121 tctccgcttc tctgtaaaaa aagaacggta atcaactggt gttaattgca aatttccttt
    18181 acgtagattt tcagcatata cctcagtcat aaattcatta aaaagatcat caatcataag
    18241 gatggaaatc aaattcacta acttttgatt atctatattt aactgattga aaagttgcaa
    18301 tccagcttca tccagttgac taactctcct tttcacgttc tgatagattt ctttttgacg
    18361 gttactgctc actgcaaaaa aaatattttc atgttcattt ttctctttaa tttctatatc
    18421 tgtatatcct tctagtcgta attgaatata taacttgaac tctgaaaacc aaaagcgttt
    18481 agaaactaga ccagcagaat attttttatt caatagattg cctcctaatc atctataatt
    18541 caataagtaa acaagtgttt tttgctcatc gttagcattc attttttagt atctactata
    18601 caatacatat ttattata
//
LOCUS       DAZKMK010000055        18596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000055 DAZKMK010000000
VERSION     DAZKMK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.055"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(290..1582)
                     /locus_tag="ACG8V1_001777"
     CDS             complement(290..1582)
                     /locus_tag="ACG8V1_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0329870.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1579..2202)
                     /locus_tag="ACG8V1_001778"
     CDS             complement(1579..2202)
                     /locus_tag="ACG8V1_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329871.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2205..3041)
                     /locus_tag="ACG8V1_001779"
     CDS             complement(2205..3041)
                     /locus_tag="ACG8V1_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329872.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(3058..3945)
                     /locus_tag="ACG8V1_001780"
     CDS             complement(3058..3945)
                     /locus_tag="ACG8V1_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329873.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3961..5244)
                     /locus_tag="ACG8V1_001781"
     CDS             complement(3961..5244)
                     /locus_tag="ACG8V1_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0329874.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5927..6610)
                     /locus_tag="ACG8V1_001782"
     CDS             complement(5927..6610)
                     /locus_tag="ACG8V1_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0329875.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6628..7245)
                     /locus_tag="ACG8V1_001783"
     CDS             complement(6628..7245)
                     /locus_tag="ACG8V1_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0329876.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7255..8079)
                     /locus_tag="ACG8V1_001784"
     CDS             complement(7255..8079)
                     /locus_tag="ACG8V1_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0329877.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(8072..8632)
                     /locus_tag="ACG8V1_001785"
     CDS             complement(8072..8632)
                     /locus_tag="ACG8V1_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329878.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8806..9762
                     /locus_tag="ACG8V1_001786"
     CDS             8806..9762
                     /locus_tag="ACG8V1_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0329879.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(10067..11023)
                     /locus_tag="ACG8V1_001787"
     CDS             complement(10067..11023)
                     /locus_tag="ACG8V1_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0329880.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11127..11729
                     /locus_tag="ACG8V1_001788"
     CDS             11127..11729
                     /locus_tag="ACG8V1_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0329881.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11817..13316)
                     /locus_tag="ACG8V1_001789"
     CDS             complement(11817..13316)
                     /locus_tag="ACG8V1_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0329882.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13332..13856)
                     /locus_tag="ACG8V1_001790"
     CDS             complement(13332..13856)
                     /locus_tag="ACG8V1_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0329883.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14154..14903
                     /locus_tag="ACG8V1_001791"
     CDS             14154..14903
                     /locus_tag="ACG8V1_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0329884.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14894..15679
                     /locus_tag="ACG8V1_001792"
     CDS             14894..15679
                     /locus_tag="ACG8V1_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0329885.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15648..16364
                     /locus_tag="ACG8V1_001793"
     CDS             15648..16364
                     /locus_tag="ACG8V1_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0329886.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16466..17485)
                     /locus_tag="ACG8V1_001794"
     CDS             complement(16466..17485)
                     /locus_tag="ACG8V1_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0329887.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17504..18505)
                     /locus_tag="ACG8V1_001795"
     CDS             complement(17504..18505)
                     /locus_tag="ACG8V1_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0329888.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         5565 a         3920 c         3261 g         5850 t
ORIGIN      
        1 gttgtttcaa atattgaaaa cagtctaaca ctactttctg aaactgacaa cgtggtttac
       61 ttattttttt gtaaaatcta atgattttta atgcattatt cctttttgtt cgggtttaaa
      121 tagttatttt tattttctcc tatctaaaaa agcagcccac ataaaaagaa ggcatgacaa
      181 aaagcatgtc atagcccctt tttccgaata aatagtgttc aaaagacagc ctatcggcaa
      241 taaggcaaga ttttataaac tacgggaagt aatttccgta aatctattct tattctttta
      301 agctgacaag ctcgtcaatc actactgctt ttcctgaagc aatagaatga tttgctgcga
      361 ttcctgtcaa aatcgacaaa ataccatcta cgtgagttgc tgcccgattc cacggatctt
      421 cttctttttc ttcaccaaat agatcatcta ataaacgcgg atctcctccg ccatgtccgc
      481 ccgcttcttg ttcgacgatt acctcatatg gctcaccgaa catcggatag acatggatgg
      541 actgaagaac ggctgctcct tcatcctttt tatctccacc tgcattgatg taagactttt
      601 cgatcacttt catttcgatc cgtcctttgc tgccattgaa agaaacattg aatccttccc
      661 acggcatata cgcgtttagc gaatacgtca aaatcgcacc agtttcatat ttgaccgtta
      721 cgcctagtgt atcttcaata gagatatcat cggaaaatac actgcggtca cgaatatatc
      781 cactgtcttt ttctgcttct aaatacattt ctcgcaattc ctcattggct gacaggtcta
      841 tagcaaatgg atcagcttta gctagttcgc ttccatagca tcgactgtaa aatatttcta
      901 ctccgcgttt ctccgcatta tgcatcccat aaaagtttaa tccaccgaat gcaaatacct
      961 tttggggacg gctgttcaac caaaaattca ctagatcaaa atgatgggta gatttatgaa
     1021 caagaagccc gccgctattg tgcttatttc gatgccatct tctgaaataa tctgccccgt
     1081 gttgcgtatt taacagccat tcgaagtgga tagaaaacac atcaccaatc acatcacttt
     1141 ctatcagttc ctttatcttc gtgtgatacg gtgcataacg ataattaaaa gtgactcgca
     1201 ggcttcttcc cgtctgtttt tggaccgtta ttatttcttg agctttttcc tcatcgatag
     1261 tcagaggttt ttcgcaaata acatcatatc cttttttcat tgcacgaatg atataatcat
     1321 gatgcgttcg atctactgac gtaataatga cttcagccgg ccgtacgtca tccagtagtt
     1381 gctcaaattg tgatggcata catgtcttta cttttttctg tccatacttg ttcacaagta
     1441 tccgatttgc ataatccatc cttgtttgag atacatcaca aaaaccgact agttcacaag
     1501 tttgatgata cgtagaggca attgcctcat aaaaaaaacg cgctctccca ccaagtccga
     1561 cttgtacata ttttttcttc atttaaagtc attctccttt tcttttaagc aaaatactgt
     1621 gacaaatggc agccctggat acagtaatgc agtgagtaaa aaatcatgct gtatacacca
     1681 gaacagtcca tacaaaaggc ataaatacca cagaaacccc ttgacgtttt ttgacaacag
     1741 ccagatattc cttttgatca aataaacaag actatctgct ttatcttctt tgcagatatc
     1801 aattagcgta ctaaaaaaac tatgggatac aaaaagcaac agacacaata gaaaaggaat
     1861 cagataccaa gctccaatct ttcccactaa taaataccaa tcaactgcaa aaataatgac
     1921 aaacagctga aacaacgtaa tgatgaaaca catccgtttg atttttcctt tcctccaaca
     1981 tacctttttt tctgccaagt aatggacaga tagataataa gaaggataca tcatcaaaaa
     2041 ataaatcaga tagacaacac gagcagacag cactaatcct aaagtgaaca aacatcctag
     2101 aactaaagcg aaaagagcat tactgataag actggttaaa aaaatccgac agacatagta
     2161 gttgagcttg taaagaaaca tctcttctat tggctttttc atattcagcc cttcagaccg
     2221 cttgtactga tcccctggac aagatattta ttgaagaata agaaaatcag aaaaaccggt
     2281 agcagcgaca acgtagacat cgcaaacata ctcgcgtaag tcgtactaga tccagcgtct
     2341 gcaaataatt tgatggcaat cgataccgtg taagatctcg gcttgtttaa ataaatcaat
     2401 ggtcccatga agtcgtccca tttccaataa aactggatga tgatcgtcgt gatgatcgaa
     2461 ggtttcagca aaggaaaaat aattttggta aagacactat acttgctgca tccgtcaata
     2521 atcgctgatt catctaactc tttggggatg ctgaccatga attgcatgat ctggtaaatg
     2581 aaaaatgctt gtccaaaaaa atgtggtaaa atcagcggca catacgtccc aaccaaattc
     2641 agccgattgt aaataataaa ctgcggaatc aaaatcactt gtgcaggaat catcatcgtt
     2701 actaacatga cgacgaacca gaatcttttt cctttgaaat cgattctcga taatgcgtaa
     2761 gcaatacaag tcgagctcag aactgtccct atcgtcgaaa ctgcagagat gacaaacgaa
     2821 ttcctaaaga aagtaaaaaa tgaaattcca gcaaaccccg cccatccttt tgcgtaattt
     2881 tcccattgaa aagtctctgg aatcaaattc agcgaaccac tcataatctc ttgatccgtt
     2941 ttaaaagaac ctgcgatcat ccataagacc ggataaatca tgactagacc taatagcaaa
     3001 ataaaaccgt attgaaacca tttcgttact gtattattca tttcttcctc ctctcattca
     3061 cacaacgacc catttttttg atgatttgaa aatcagaaag gtgacagctc ccataatcaa
     3121 cagcatgatc cacgaaatag cactagcata ccccatagaa ttaaaagaaa aagcttggtt
     3181 atacacataa agagcataaa agttcgtggc atcattcgga ccaccttggg taatgataaa
     3241 tgcctgcgta aaagtcataa acgccgcgat tgtctgcata accaagttat acaaaatgat
     3301 cggcgaaaga agaggtaacg tgatcttgta aaacgtctgc catttgttcg ctccatcgat
     3361 ctctgccgct tcataatacg agcctgggat ctctttgata cctgcagcaa agatgatcat
     3421 tgccgaaccg aattgccaag cagacatcaa aacaagaggg atcatgacca gtttttgatc
     3481 gctaaaccag gaaagcgcag gtgcgcctaa tcgttccagg aaaatattca ctaacccgtt
     3541 tttagaaaac aactcccgcc aaactagagc gatagaaata ctgccaccga tcaaactagg
     3601 cacataatac aaagaacgaa agaaagtcgt tcctttgata ttcctcgtta aaagataagc
     3661 aatcaataga ccaaagatca gtttcacagg tacagatgca aatacataga gaaaagtgac
     3721 tcggatggag ttcaaaaaac gggcatcact cagcatcttt atataattat ccaagccgat
     3781 ccatgagact ggatttccca aacgataatc ggtgaacgaa taataaagtg acaagatcat
     3841 cggtatcact gttatcaata gaaatcctag gatgaatgga gaagcaaata cgtaaccgac
     3901 cacattgtct tgatccaaga accgttttag tttttctttt ttcatgcctt cctcctgctg
     3961 ctatcctaga atgtttgacg caaactcata aagactctct gctgcttctt ctgctgttac
     4021 ttcttcataa atgacttttt caactaaaag attgtattgg tcttgaattt ccggattcaa
     4081 agcactatca aagacattca cttcgccgtt gtccatatcg gaaatcattt ctaaataatc
     4141 aaatgtcgct tgcaaagttg catcatctgc acgagcgagc tgttctcgaa tattcgacat
     4201 gattggtaca ccacgttctc cttttaaaat ttcattggct tcttcactgt tttgaaagaa
     4261 atcaataaac ttagcagctt cttctttatg ggctgagctt tttgccatac tcagcatttg
     4321 cgaagaacgt aaagggatac cagattctcc atctgctttt ctgcgaggtg gattaatcaa
     4381 ctgaatctcc atatcttcag gagcccctga caatatttct gtcaactggt tacttgatag
     4441 aaacaccatt gccgcttctc tatccctaac cgcctgaaaa gacagatccg tcgtacttgc
     4501 agctccacca cctggttctg gatgggcccc tttgtctacc agatttttac gcagctggaa
     4561 aaaatctgtt agatagtctg gattttcaaa tcccaagctg ttagatagat cttcccgatt
     4621 ataaaaattc aatcctttct caacttgtgg tatttgaata atacagccag ctataaaatc
     4681 ttcgaatctt gaaatagcat agattcctaa ttcttcagtg atttgatagc tgatttgttc
     4741 aaattcatcc catgtccaat ttgaagctgg ttcagggata tttaattgtt caaatatatt
     4801 tttattgtaa gcaatcgcaa acgaattcac cccgttagac agtccaacta attgcccgtc
     4861 gtaagtcgtc gttgctaaat aggattcatc tgcattagaa atatcaatta tcccttgctc
     4921 cacatattca tctaatggtt caatctgatt gatgtattga ggaaattgat tgttcagttg
     4981 gaaaatgtcc cagatatcat ctgatgctgc tagtgtacct aattttgtgt aatatccttc
     5041 ataatcataa aattcataat cgatcgtcac atggggattt ttttcttcat aaagctcgat
     5101 tacttttact gttgcatcgt gtcggacttg cgaaccccac caagccatgt tcagcgtgat
     5161 ttttttgtca tcttcttgcc cacaagccgt caagaagaat aacataacac ttaaagctaa
     5221 gatgattctc attcttttct tcattctctg cacctctact tccattgtta tttttcaata
     5281 tactgtattt ttatacagaa catatttatt aaacgatctt tttcaagggg aataaaaacc
     5341 aagacttgcc ttatcgttct gcctaggatg aacaactcaa tactttcaag aaaaattaga
     5401 tagtgctgat tcttctttca tcgctaaaaa cacttcttgc ttcaatgatg ggaggataat
     5461 tacgcaaaga aaaaaggata tcagacaact gcccaatatc cttgacatag ctaaataagc
     5521 atgcttcttc gtgtgcgctc ttccctaaaa aacagaaaaa taactagtaa ttctgtatga
     5581 aattaggtac aatatacacg tcgatgcaca agcattgact atggcaactt gattggtcag
     5641 ttcccgctga cttttcaagc cccgacaatt tagctgctac taaattgtcg ggtgcctttt
     5701 ttagttttta ttcgtttttc attctataga aagcgcttca ataaatcaag acattttttt
     5761 gttagaatgc taataataac aaaaggtttt aactcttctt atgtacatta caggtaaaaa
     5821 aacaaaaaaa tttctctttt ttttgaattc tgaacaaata gtactggagt acaaaagacg
     5881 aggaagagcc actatctgct cttcctcgtc ttttgattct cacctgttat tcatgattct
     5941 ttttctcagt ttcccgatat ttttccattt tacgactaaa aagctctccg ctacttggtg
     6001 gcgtatatgt gatggcattt gctcctgctt gaatggtctc agcaatactt tcttccgttg
     6061 gaccacctgt cgcaatgata ggaagttcag gatatttttt acgaaaatgg cgtactgctt
     6121 ctgcagtttc tttacctgca ctgatattga taatcgaaat accagctgct aacttttcat
     6181 ctatttctgt atatttcgac gttaccgtac tgataactgg aatatcaact actcgacaaa
     6241 cttcttttac tgtctctaaa ggcgtaggac cgttcaaaac gacccctaac gaaccttgcg
     6301 cttctgcgaa catgctcatg taggaagaac gagcaccttg tgtcaaacca ccacctacac
     6361 cggagaaaac tggaatgtca gctgcctcaa taatgctttt agtaatcgct ggatgaggtg
     6421 taaaaggata cacagcaatt actgcattcg catttgtatt acgtatgatc gcaatatccg
     6481 tagtaaagat gatcgaacgg atcaattttc caaatatctt gatcccgctt gcttctaaaa
     6541 tgatttccgg tactcttacg atatcttttc tcaattccgt catgatttct ggcgtaaatt
     6601 gttctgccat cagccacttc tcctttgcta tctccggtca tactgattca attgatacgt
     6661 ctcgatgacc tggatgattt tttgcgcata accggggtct gtcgcataac ctgcatcttg
     6721 caatgcttgt gcggcttcct gatagttggt ggccgtaatc actttttcat acagcgcagg
     6781 attccaatct actccttgga cgaacagttg tgtatggtca tccatggact gttcccatga
     6841 atcatacact ttgaagtctc cttggatagt tatccattct tcgttaacaa attcctttgt
     6901 ttccaggctt actttcttct gatccccata agccttgatc ccgaaaagat tattgtattt
     6961 gctagccaag gtactttttc cccaatttga ttctaatata gcttgtccta aaatgatact
     7021 aggaagcact ccataaccat cttgtagttc ttgggcatgc ggaacaagcc gttcaataaa
     7081 ttgctgatgg gattgcgctt cattttcttg ctgattatca gtaaaaggag aagacagatt
     7141 cttcaaggaa aaaacaaagg ctaagagtat cactgctaca ccggcaacca ctgctggcca
     7201 acggatattc ttgcgctttt gctttttcca cttttttttc gccatgatca attcctactt
     7261 ttcatttaat tcattcagat attcttctag cgtcaattgg tgtttggtga tatactcagc
     7321 gttttcttta ccgacataac gtaagtgcca aggttcatat gtgatcttag ttatatcttc
     7381 gtgtcctttt ggataacgaa cgataaatcc atactgcgca gcattatctg cgatccattt
     7441 agctccaggc tgatctccgt aacttgcatc caatacagta gaaggataac tgttgtacca
     7501 gttttgatcg acgacatcga ctgccagacc tgtgtggtgt tcgctgtatc ctggctccgt
     7561 aatcgtttct ttagtaatct ttgtcgcctc ctcttctgat acaccttgac gactcatcac
     7621 atcttggaca ttttgttcga aaacttgttg ttgggatgat acagaccggt aagctgagac
     7681 catcactaat ggaaatccag ctgcttcagc agccttggca aatgcttcat attctggagc
     7741 aatgcgtttg tcgatcaagt atccgttact taatgatgcc aattgagtag actcatcaat
     7801 ttctttttct aatttgtgat ctggaccaac caaaactagc ttccaatcgt ctgttttaac
     7861 atctggcaat tgtttggacg tacgatcttt tgtttctttg tcctttacct tgctgctggt
     7921 tgtttgactg ttcttttctt tactagcagt tgttttggtg cttgttcgtt cgactgcttg
     7981 attgccggct tgaacatgcg aatcatcatc gctaaaaata acatagccca ttgctgcgat
     8041 cataatcaca actgctccaa atcctaaaaa tttattcact tgaaaaatcc tttcgttgtt
     8101 cttcacgttc cccattcgcc tcatgaacgt aagtttcttc cagattttca ttcacccaat
     8161 cgagaagttc gattttttct ccttcgatct gtttttgaac atctgctggc agacgaaaat
     8221 tcaaaatatc atcatagccc cattcatcat attgaatact tactttcaag tcaaggtatc
     8281 taccagcctg ctgattattt ttcaaaggga tcagctcgac ttttgctgct ttgctgctaa
     8341 gccatttttg cgcgatgcgt gttttgattt gcttgtactc tgcaacggca acgtttcgtt
     8401 tttcgggaaa aataatcgtt tgacgcaaaa tcttttgcgt tagcatccat tcataatcag
     8461 tgaaaagatt ttttactttc tgcatcgctt cagatggcag atttttctcg ccatctttcc
     8521 atttttgcca ttgttctgga ctgatcagca agtattcatc taaaaaattt ttttcagttt
     8581 caaactgttc taataataca ctcataataa gatcaatata tacttgatgc acgtttatcc
     8641 cctcctttta actattctac ttaagttttg atcaaaaagc acctcttaaa tcgctgcttt
     8701 catttttttt aatgtttcaa gtcctctcct ctttattctt cgtgattttt gttataataa
     8761 tagacagaca agaaactcaa tccaactatg aaggagcgtt ttcagatgaa aaaatatcaa
     8821 tggggaatcg ttggtcttgg taccatcgca catgagtttg ccgaaagctt caatcaagag
     8881 acgagcgaac taacagcagt tgcttccaga acttcagaaa aagcggaaaa cttcgctcac
     8941 cgttacaata ttccaaaagc ttacggaagc tatcaagaaa tgttagacga tgctgaaatc
     9001 gacatcgtgt acattgctgt accaaataga cagcatatcg accatatctt ggcagcattg
     9061 gaagcaggca aacacgttct atgtgaaaaa gccatcacga tgaataaaaa agaactggct
     9121 gacgctatgc gcttagcaga agaaaagaat ttgatcttag cagaagcgat gacaatcttc
     9181 aacatgccac tttatcagca gttacgctca ataatggata caggaaaact gggggcttta
     9241 aaaatgatcc aagcaccttt cggcagttac aaagaaccag accctaaaaa ccggttcttc
     9301 aatccagaat tagctggagg agctctgttg gatatcggta cgtatgctgt atctttcgct
     9361 cgtttctttt tgagctctca acctgaagta gttgcctcta caatggttcc ttttgaaaca
     9421 ggcgtggacg aacaatccgt cactatcttg cgtaacaaag aaaatgaact tgctgctgtc
     9481 agcttgactt ttcaggcaaa aatgccaaaa gtcggtatcg ttgcctttga gaacggttat
     9541 gtgacgattg ctgattaccc gcgtgctgat cgtgcggaga tcttatttac agatggtaca
     9601 aaggaattca tcgaaagcgg gaatacatct gaagcaatga attacgaaat aaagaatatg
     9661 gtacacatga ttgaaggtga cttgcccaat cgctctctct tcttgacaaa agatgtcatc
     9721 gaaatcttgg atcaaatgca agttctatgg aaaaatatgt aattttaaaa ggttgtgaaa
     9781 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagctcctc ctatttttcg
     9841 tttggctgga cttattaccg cagaggaaag cttttgaaca ccgtttaaga aagaggttgt
     9901 gagcctaccg ttcagctccg agtcacaagg agcaatttca aaaaacagct tctcatgttt
     9961 gttgaatatt gtgacttgtg accgaggagt tgacaggaga acaccgttta aagaagaggt
    10021 tgtgaaatca gctttttgac ctgaatatta gctgaattca caacctctat ttttggattt
    10081 tttgttggga gaattctgcc ttgatttcaa taaaactttt aaaagactgc aaaaactgga
    10141 acaattctgc tcgatcctcg aattcagggc gactagtcgg cagaggcatt tgccgataca
    10201 atacataaac ttcttcaata cgtgttaaaa tttgtctgcc atcatttgac tctgaaaatg
    10261 tttctgcagt ataaatcaat aaaccataga tatgttttcc ataataagct ggttgctgga
    10321 ttctctctag attttccagc atgtctttaa tcacattggc ctgtgctctt ctcatagaga
    10381 agtaagtttc gtagtatagc ggctgtctga gccagtagtt ttcttgatgt tctctggcat
    10441 cttcttggct ttcgcgaatg aatgtcagta aatgttcgca tttgatctgc acatcttctt
    10501 tattttctga taaaagaaat tctgccattt gtctcaaaat atttcgaaat tctttttcga
    10561 tctgtatttg gttttccttc agctgcttcg tacgatctgg catgtaaaca tttgcaagca
    10621 aagcaaatac taatccaatc gccattaatc ccgcttcatt gccgatcagc tgccaagaca
    10681 tgttttcctc cactaaataa tgagtgacca aaaccgaatt tactactatc ccttcggtta
    10741 atttgaagcg agaggcaagc gggatgaaca ggaataaaaa aagactgaag ccaaaaatag
    10801 tatacccaac agcaaaaaaa ctgaacagtg ctgttgccgt agctaatata aaggctgcga
    10861 ttcgattcat gcccgtacgc aatgtagagc gcttggtatt tcctacactt aataatgcaa
    10921 tgatccctgc agctggccaa tacaacagcg ataagctacc cgctgtgacc atcgaaaaga
    10981 agacactgat cactgttttg actgttcgca ttcctatacg catttacttc cccctttttt
    11041 attttaatta tacttgaata atgaaaaact agctatcaga caaaagttta ggtatgatag
    11101 taaaaagacc aaaaaggaga atctctatgc aatttggccg ttttcgacta ggtatgcgta
    11161 cgatcaaaag tgccttagct gtttttttat gtatcttgtt ttttcatttg actcaacgag
    11221 gaatcccgat gattgctgca ttatctgctg ttttttcttt gcgtcaggac ttgactacaa
    11281 ccgtttcttt tggaaagtct aggatcatcg gcaatacatt aggtggtaca ttagctatca
    11341 tttatttttt tgtcaaaagc tattttcaca atgatttttt ggtagaattg atcttgcttc
    11401 ctattctagt tattattgtg atcgtcgtgt cagatggtat taacaataat tccggtatca
    11461 tttccgcaat agctacgttg ttgctgattg cgttgagcat ccctcaaggc gaatccgctt
    11521 tgtatgctat ccaacgtgta ctagatactt ttatagggac attcattgca atcggtatca
    11581 atttctttct tcgtcctcct gaaacagaaa agaaggaaga actatcagaa gatttggtcg
    11641 aactccaaaa gaaagaacga ttgctaagaa caaatttaca agagatagaa gctaagatcc
    11701 aaaaacaaaa gaaaaatagc gaggactaat tttcttttgc aataaaataa aaaaatggag
    11761 agaagaactt taaaaagtct tcctccattt ttgattgaac ggtgaagtgt actactttat
    11821 tcttccttca tcgagttttg ttctgcctct tttggcgctt catacatatt tacttggcta
    11881 gtagtcccac gtgtcgacaa attctctacg atttccttca agtcaagtcc tgttgtcgct
    11941 tttagtgttt cttgagtaga agaaagcaaa tttgtggcat aatttgtcac tcggttcgct
    12001 ccactggttt ctcctgaact gcttcctgta tcgacaacag agattttttc gatatttcct
    12061 agcggctgtg ctgcttcgcg catcaattgc ggtagcatat cgataaccat actcaagaca
    12121 gctgcttcgc catattcctt gaaggcattg gcgatctttt gttttgcttc ggcttctgct
    12181 tttccttttg ctagtgctgc ttcggcttct gcttgtccag ctaaacgtgt cttattggct
    12241 tccgcttctg ccagtgcttc cacacggaat cgttctgctt cggcttcagt cacttctttg
    12301 actttttgag cttgtgcttc ttgttcttta gcgtaccgat cggcatctgc ttttttcttc
    12361 acttccgaat catattgctt ttcacgacgt gtgatttctt tttcttccag ttcgatttgt
    12421 ttttgacgtt ctacgatttt aatctgcatt tcctgttcga ttacttgttg ttgtgctcgg
    12481 gcactttcta aattgtaggc ttggtctgct tttgctttag caacatcttg ttcttgtttg
    12541 taggttgcta atttcagttc tttttctttt aatgattctg cgatctctgt ttgtcgctgt
    12601 aattcagctg cttgtgattc tttttctgct tgagcttttt tgatacgcgt ttcttttaga
    12661 gcttccgctt ctgctatatc tgcatctctt ttgacttggg cgattcttgg tttccctaaa
    12721 gagtcaagat aaccgttttt gtctctgacc tctttgatag tgaaagaaac gatgatcaac
    12781 cccatttttg caaggtctac agaagcgact tcttgtacgc tttgactgaa cttatcgcga
    12841 ttttgataga tttcttctac agtcatagaa ccaagaatcg agcgcaagtg tccttctaat
    12901 acttctcttg cttcattttc cagttcttct cttgtcttgc ctaaaaactg ttctgctgct
    12961 gtagcaatct cttctacaga tgaaccgatt ttgatgatcg aagtaccatc acacatcacc
    13021 ggtacgcctt gttccgtata cacttcaggg gtggagacgt ccaatttact cgataataga
    13081 ctgatccgat tcgaacgttg aaaaactggc aagacgaatg cgccacctcc acgcacaatt
    13141 ttgattttgt tattgctttc atcaatgtgc acatttttgc ttcctaaata acttccactg
    13201 atgatcaacg cttcatccgg ttttgctgtc tggtacttcg ctacaaagac gatcagcaac
    13261 atcagtaaaa tcaaagctgc gattgcgatc gcgataaaaa caactggtat ttccatttaa
    13321 cacacgctcc tttaagcttt tatcatgctt tcataaggca tgacataaca gattctttcc
    13381 ttgatctcaa tgatcaatac tttcgttccc cgctcagctg tctgttcttg ggggctgtaa
    13441 aaacaagctg gacgactaat ggaaccggta acacttttca actgaatttc tcccatacct
    13501 cgtacaggga tcggtgtgat cacaacagct acttgtcctt ccagagtttt ttcagaagac
    13561 gacatcgttg attcggcatg tttcatcggc atcaatatat agaaattaag caaaaatacc
    13621 aacactatcg atatgcctcc actagcaata aataaaaata aagatgattg atctgttaaa
    13681 cgctcaccta gaaaaccaaa taagcttgtg aaagttaacc aagggataat aagcatcgga
    13741 tcaagcgggc catcaaaatc gaatacatct ccaaaaataa tcaataggac tgccaatcct
    13801 gcacaaatca gcaatgtata ccaataaatc gtctcaagtg cgattccccc tatcatatac
    13861 acgcctcctt ccatttctac gtctatcata ccaatgtttg aaaattctgt atagcttctt
    13921 ttaccgactc agtggtgttt tttataggaa ggttgtgttt atcgttgaca atttgttaaa
    13981 tttttgtttt catgttaaaa ttttcacaat aaattctttc gaggtagcct atgtttcctt
    14041 tttttaagaa aaaaccatta aagctccaga acacgatcat ccttgtcgtt tgtttctcca
    14101 cattcgtttc tttgattgtt tctgccatat tgatcaggaa ttttattctt tcgatgaacc
    14161 gatctagtgt gtcagataaa atctctagca ttgctaaaat cgttgccaat gataaactca
    14221 tccaaaaaaa gctgcagact ggtgacccga cacatgcgat tcaaggaata acaaatgaaa
    14281 tcatgattgc atcgaattta gattttatcg ttgtcatgga ccctgaaggc attcgatatt
    14341 ctcacgcaga cccaagttac atcggtcttc ctttctcaag tcatgaagat gtgaaaaaag
    14401 tgattggagg acaagaattt ttctctgaaa aagaaggaat gttagggttc ggcttccgct
    14461 atttcgtccc tatttattat gatcaagaga ttattggtgc agtttgtgtt ggcttaacaa
    14521 acaacacgat tgacaatcaa gtcaaagatg gacaaaaaat gattgttttt tcattgatta
    14581 ttggacttag cgtaggcatc ataggcgccg tttttcttgc ccaaaaaatg aaaaaagtac
    14641 tgcttggact tgaaccacaa gaaatagcag aacagttaag tgaaaaaacg attatcgaaa
    14701 atgaagtaga tgaaggaatc ttagccatct cttctgataa aactatcctc ttgatcaata
    14761 cacctgcaaa aaacattttc taccaaacag agcagctatt ggatttcgaa gtaggaaaaa
    14821 aatcagtgat aatctatatg acgttttgtt taaagagacg attgagcatc aaacgaccgt
    14881 tcggactcaa tccatgtatt taaatgggat agaaactatt tctagcgtta cacctctttt
    14941 tagcaatgac cgttttttag gagcagtcgc aactttacga aatcgatcag aagtaatgaa
    15001 attaatggag gagcttgggg ggacgaaaca atatataaac tccatgagag ctcaaaccca
    15061 tgaattcatg aataaacttc atgtcatttc tggtttgatt gatttgaaga agtatgaaga
    15121 agtcacccat tttatccagc agctaaatca aaattataaa aaagagattg gccaagtctc
    15181 aagtctgatc aaagtacctg caattgccgg ttttttatta ggcaaaataa acgaagccaa
    15241 agaacaatct gttgaaatgc tgttagaaag cgattctttt atccctaaaa tggagatgga
    15301 tgactccatc cattcacttt tgcaagtgtt agggaactta cttgataatg caaaagaagc
    15361 cgtgctacaa aaacatgaaa gaaacggaca ggtccggatt catttaaact atgacacaga
    15421 agctgaagta ttcatcgtga ccgtctgcga tgacggtgtc gggatatcag aagaaatctc
    15481 agaaaaaata tttacgctag gtttttcgac gaagggaatc aatcgtgggt ttggattgaa
    15541 tctcatccat tcaatcgtca aacgccacgg tggattttta gaattttcca aacaaacaaa
    15601 tggcggtaca atcgtctatc tagaattacc aaaaaaggaa gatcaaaatg atgacaaatg
    15661 tcctgataat cgaagatgat ccaatggttg cagaattgaa tcgtactttt gtggaaagca
    15721 tccctaatat gaacgtaatc gaaagtttca gaaatgtaga agaaggaaaa aaatatttca
    15781 gacaaaaaaa cgcagatctg ctgctgcttg atgtatacat gcctggcgaa agtggtatcc
    15841 attttttatc ttggcttaga cagcaaggaa atgatatccc agtgattttg atcactgctg
    15901 cagatgatat cgaaaccata aagaatgtga tccgctacgg tgccttagat tatttgatca
    15961 aaccgttcac tttgcaacgt tttcagctgg cgatcgaaaa gtatcttgct ttctctcaga
    16021 caatccaaac gactgaaaaa gccgatcaga aattgatcga tcaagtattc aaccacacca
    16081 ctcccattca cgaaatacca tcattcatcg atttgcctaa aggtctgtca aaactgactt
    16141 taaaaaaaat aagtgcattt attctaaaag aagatactat tttttcaaca aaagaactgg
    16201 ctcaaaaaag ctcactgtca cgtatatccg tcaagaaata tttgaatttt ttagtagaac
    16261 aagatatatt gaacgaaaga atcaaatatt tggaaatcgg ccggccgcta acctgttatg
    16321 aattgacaaa gaatgggcgg aaaaagctca cagattactg ttgagtcatc gaattatccc
    16381 atacaaaata gtccaagtat acagaagtaa caactgttta cttggactat ttttaaacat
    16441 tttattgacg aatatccatg ttttcttatc ttaattgacg ggctttagga agagatacct
    16501 catttttcgt ttttccagtt tctattattt cacgaaagtt ctcgaaactt gtcgaaatca
    16561 tatttgataa tgcctcatct gtatttgaac ctacatgcgg ggttacaaga actctagggt
    16621 acatttctac taattgctgg atagcagggt ctggaatcgt ctcccatggc ttgaaggatc
    16681 ggaaaaaaac ctctgtttca tttgcaaaaa catcggttgc aaaaccagct aaatgattgc
    16741 ttttcaaagc tcttaaaata gcttcattat cttgaagctc tccacgggct gtattaatca
    16801 agatgctacc ttttttcatt tgagctaaaa atccttcatt gatcatttga tcgttttcac
    16861 ctgggatatg aggtacatga atactgacaa tatcgctttt ggctaataac tcttcaagcg
    16921 agtggaacgt caatactttt ttagcagctt ctgtctgatg aatatcgtag ccgatcactt
    16981 tggctcctag tcccttaaac aaaacagctt ccgtcaaccc tatcctgcct gttcctatga
    17041 tccctactgt gcaatttctg atttcacgac taaacatttg ctcatccact cgaaaatctt
    17101 tgaaagatgt tcggatcgtc gtatacgcag tatttctcaa aagcatcatg gcaaaagtca
    17161 aagataactc tgctatcgca tttggcgaat acgatggtac acgggctact gtcataccaa
    17221 aatcagctgc cgcttgtaga tcgatatgat tgactcccac agtccgtgta aaaacataac
    17281 gaatcccata ttcctgcatt tttgcaagat tggtctcatc tgctatacaa tttcccctca
    17341 acaatacagc atcatggtcc tttgctgaca cgatattgtc atgtgtcaac agttcttcat
    17401 aaagactgag atcataatgg taaatattca atttttcaaa aaaatctatc tcgttcggac
    17461 gaacaccgta acatgcgatt ctcatacttt cttcctccat ttcttatgaa aataaaccta
    17521 tgccatgaat catttgtaat acaacgatcc atacaggtgt cagtaccaca gcaaaaatag
    17581 ttgacagtaa ggatgcattg gaggctaaaa gtgcctccct atcaaaactg atagcatagg
    17641 ctgctgccac tgttgcagct ggtgtagcca tcatgataac gattgttgcc agtgcttcaa
    17701 aattgactgg tagaatattc gttgccgtca aaactgctag aatcacgata ttcaatgcag
    17761 gtacaaatac cactttgact agactgtaat accatgaagt tttttcagat gcagcttttt
    17821 tgaagcttac ttcacctaat gttgcgccga ttgctagcca cgccaatggt gaacttaaac
    17881 cagctaaata atccatcgac tgaaatagcc acggtgcagt ctggtcaatt cgcaaaaacg
    17941 ctacttggct tatctctcct tctgcacctg taactgacac ttgtggcaga tacccttgga
    18001 acagccaaat gaaaaggccg gcaaaagtag cgataatgat tggattgacg aacatcgctt
    18061 tgatattttt agcttccatc ttcaagccac tcatttttat gtatccataa gaatacaaaa
    18121 agattcggta gccaatattg aaaattgagg aatacatcac gccgacagga ccataaatcg
    18181 cactgacgat aggcgttccg aaaaaggtcg tagaaccaaa gatcgtcagg acacgcaacg
    18241 ttgtttgctt atccccttta tagcttaaga aaaaaggttt tgaaactaga ataagaataa
    18301 tataaataac taatccccaa atcaagacat tgattccttg catcagtaat tctgtattga
    18361 tatcttgcat gaaagctcga aatgccagtg ctggcaaagc cactgttaag acgactttcg
    18421 atagaatttt tccaacagat tcagaaaaaa tccccttttt tctacaaaca aaccctaata
    18481 atataataaa aactgttgaa gtgattgcac tgacaatcga catatctgtc aaagtcgatc
    18541 gaaaaatgtc aaataagttc atctttctcc tcctaattat gttcatttat tcacaa
//
LOCUS       DAZKMK010000056        18565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000056 DAZKMK010000000
VERSION     DAZKMK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.056"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..394)
                     /locus_tag="ACG8V1_001796"
     CDS             complement(<1..394)
                     /locus_tag="ACG8V1_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329889.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLND"
     gene            661..2361
                     /locus_tag="ACG8V1_001797"
     CDS             661..2361
                     /locus_tag="ACG8V1_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0329890.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2331..2549
                     /locus_tag="ACG8V1_001798"
     CDS             2331..2549
                     /locus_tag="ACG8V1_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0329891.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2569..3459
                     /locus_tag="ACG8V1_001799"
     CDS             2569..3459
                     /locus_tag="ACG8V1_001799"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0329892.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3477..3761
                     /locus_tag="ACG8V1_001800"
     CDS             3477..3761
                     /locus_tag="ACG8V1_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329893.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4248..4862
                     /gene="gmk"
                     /locus_tag="ACG8V1_001801"
     CDS             4248..4862
                     /gene="gmk"
                     /locus_tag="ACG8V1_001801"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0329894.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4867..5172
                     /gene="rpoZ"
                     /locus_tag="ACG8V1_001802"
     CDS             4867..5172
                     /gene="rpoZ"
                     /locus_tag="ACG8V1_001802"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0329895.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5425..7833
                     /gene="priA"
                     /locus_tag="ACG8V1_001803"
     CDS             5425..7833
                     /gene="priA"
                     /locus_tag="ACG8V1_001803"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0329896.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7848..8339
                     /gene="def"
                     /locus_tag="ACG8V1_001804"
     CDS             7848..8339
                     /gene="def"
                     /locus_tag="ACG8V1_001804"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0329897.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8332..9270
                     /gene="fmt"
                     /locus_tag="ACG8V1_001805"
     CDS             8332..9270
                     /gene="fmt"
                     /locus_tag="ACG8V1_001805"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0329898.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9270..10628
                     /gene="rsmB"
                     /locus_tag="ACG8V1_001806"
     CDS             9270..10628
                     /gene="rsmB"
                     /locus_tag="ACG8V1_001806"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0329899.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10641..11381
                     /locus_tag="ACG8V1_001807"
     CDS             10641..11381
                     /locus_tag="ACG8V1_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0329900.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11378..13447
                     /gene="pknB"
                     /locus_tag="ACG8V1_001808"
     CDS             11378..13447
                     /gene="pknB"
                     /locus_tag="ACG8V1_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0329901.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13612..14511
                     /gene="rsgA"
                     /locus_tag="ACG8V1_001809"
     CDS             13612..14511
                     /gene="rsgA"
                     /locus_tag="ACG8V1_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0329902.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14524..15174
                     /gene="rpe"
                     /locus_tag="ACG8V1_001810"
     CDS             14524..15174
                     /gene="rpe"
                     /locus_tag="ACG8V1_001810"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0329903.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15175..15813
                     /locus_tag="ACG8V1_001811"
     CDS             15175..15813
                     /locus_tag="ACG8V1_001811"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0329904.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15968..16822
                     /gene="mreC"
                     /locus_tag="ACG8V1_001812"
     CDS             15968..16822
                     /gene="mreC"
                     /locus_tag="ACG8V1_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0329905.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16826..17335
                     /gene="mreD"
                     /locus_tag="ACG8V1_001813"
     CDS             16826..17335
                     /gene="mreD"
                     /locus_tag="ACG8V1_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0329906.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17590..>18565
                     /locus_tag="ACG8V1_001814"
     CDS             17590..>18565
                     /locus_tag="ACG8V1_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0329907.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         6296 a         3363 c         3983 g         4923 t
ORIGIN      
        1 gatcattaag atcgaagtat acaaaattat tctgtagttt ataaaaaaac ggcgaatgaa
       61 gatgtgtttt catatgacca aaatataata attctgaaat ttctaaaggt gtcaattctt
      121 tcagcatcgt taaatcagta aagtcgatcc acttcaattc atcgaatcca cgcctgttac
      181 gagaagagga aaagaaacgc tgaatatttt ctgtaccacg gattactttc agccctgtat
      241 gggattcata ttctccagca gaagcagatg gatcaagcaa gagcaagttt tgaggaaaat
      301 gcacgatact gtttgcaaag tcagtagcag tcagcccttt tgttaaaaca gcattacttg
      361 tggtatcaat atgtacataa actgactcag tcatcatgtt cctcctctta tttcctctta
      421 atctctttta ttttacaaca tttgcattaa aaaagcttgt ttcttttatt ttaaaattcc
      481 attacaaaat cacgttttca ttgaaaacac tctcattttc aattaatttt tgtgattttt
      541 gtattttttt atttaaagga ggttttatga attgttttct tatggaatct acaaagtcga
      601 atacgagact catgaccttt ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg
      661 atggagataa aaaaagttta cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa
      721 gcaaaacaac cccttccatt ttttcaaagt catcaagaaa gtagagacgc tttttggcgt
      781 tctgcaaaaa agcaatgtgc ttttaaagca gtatcgaaaa aatataccgt ctattttact
      841 attgctaaaa gtagaggaga tgttattttt aataatctgt tgattgaagg tgaaactttt
      901 gaatggaaaa aattttttcg gtatatggaa gcttgcgctc gtttttttat taaagaaaac
      961 tgttgttttt tatttcaatc tcctctatca gaagaatggt tgcgtatttt tcataaaaac
     1021 ggctatcaag gaaccacaca attaaataaa aagctggtct atcatacagc acttgtttta
     1081 ggtggaggag gagcgcacgg tgcttatcag attggtgtgt ggcaggcgct gaaggaacat
     1141 gatatcaact ttgagattat cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt
     1201 ttacaaggag atatgaaaaa agctgtgaat ttatggaaaa agctgtcgac aagacaagtg
     1261 cttgcgttac cagaaatggc tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga
     1321 cgccaaatga cgaaaacagc actaattgaa ggcggggttt cttctgctcc attggaaaag
     1381 atggtcaaag acaatttgga tttgtcctta ctcaaacata atcccaaaat acgtttatat
     1441 acggtgtcta ctaaactgcc agaactagcg gaagtagtga ctgatatcca acagacaaaa
     1501 acagaagaaa taccagattg gatactcgcc tctgcttcat tttatcccgc tatggcttat
     1561 cgaaagatcg gaaaaaataa atatgcagac ggtgggtatc gcaacaaaat acctattgat
     1621 attgcgatta acaaaggagc aacagaggca tttgtcgtag atgtgcaagg gccaggccca
     1681 gcgaaaaaaa ttcgtatgcc agacattttt atccattgga aatgtcaaac attgtggaca
     1741 ctaggcagct ttctgctttt tgatagccaa aggaaccaat taaatttaca attaggctat
     1801 ctggaaatga aaaaacgatt aggctattac tatgggaact ggtatacatt tcattctgtt
     1861 aaacctgcaa gaacatactg gcgaggattt ttgtcttact taacaaaaga aattcagtta
     1921 gaattatctt ttttcaaaag cataaaattt tggcaaaaac tgcgtaattt atataagaac
     1981 agggtcgtac cagagacatg cggattggcg atgctagaac ttttagcaaa aaaacatttt
     2041 ttactgccca atgaaatcta tcaagtagac atgatgattc aaatgatctg ccaacaagat
     2101 ataaaaagta tgccggatga tttattggca caaattggtc aattaagtac tgaagaatgg
     2161 cgaaggtatc gaaaatacca gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt
     2221 gattatctgc tacaaggaaa acgcaaggat cgtttgcagc atgaattgct taagcaacca
     2281 gttgacacat tactgatcct atatttatat tatctcaagg aggaacaacc atggcacaag
     2341 aattttcata tgaaatcgta gaagaaatcg ctatattgtc agaaaacaac aaggggtggc
     2401 gtaaagaact gaacctagtc agctggaatg gtcgcccgcc aaaattcgat cttcgagatt
     2461 gggcgccaga ccatgaaaaa atggggaaag gcctgacatt gacgaacgaa gagtttgaac
     2521 aactgcaaaa agcaatagaa aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg
     2581 actggttttg gcaaagccat ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa
     2641 agtgtcaacc agcgttttct agatgttcaa atacgaagtc ctaaaatcct gaactttgtt
     2701 gaaaacgatt ttcgtcagct tattaaacag cagttaagcc gcggtcgaat cgaagtattt
     2761 gtgaatctag cttatattgg caaaaacgaa aaacagatcg tcatcaactg ggaattgatt
     2821 gattcactta tcactcaatt gacagacgga attcaacaac gatacggtag tcaaaatcaa
     2881 gtgtcaatta gtcggatggc ggaacgcttc gtgctggacg aaaacttcgt aacggtagaa
     2941 gaaaaaccgg aagagagcat ggaagaattg gcacagttag ctctagatac attgaaaagc
     3001 gcacttgatt ctattgaggc gagtcgagaa aaagaaggtc aagctttagc tttagtgttg
     3061 aaaaagaata ctgaagaatt caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc
     3121 tatgaaaagg aatttcaaat aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa
     3181 gtcgatgagc aacggttgtt gacggagcta gccatcttgt tggaacgcgg agatattcac
     3241 gaggaactgg atcgtctggc gatccatgta caaaaactag atgaacttat cgaagcacaa
     3301 tgtcctgtag gtcgggaatt agatttcctg atccaagaaa tgaatcggga aatcaacacg
     3361 atcggttcca aatcaaatgc aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc
     3421 ttagaaaaaa tcagagaaca agtacaaaat attgaatgac gaaagaggta gagataatga
     3481 aatattatga cgtaacgttc cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc
     3541 cttctgaaaa aaacggcttt gacgtatgga aagatgcctg tgcagactat aatgagaacg
     3601 aactgtttat tttgatcaac gacggtgctt acgtgacgat gaatcggaaa tttattgttc
     3661 gaattgacac agaagaggta gaagatccga cagaaaaagc acgttctcga aaagatgaga
     3721 tcatgggtgt agtgaatact ttgtccaata tgggctttta gcaaggatgc tgaacaaaga
     3781 aatacaataa ataaacagaa ttggatttct tcgcttagct tctttgctct aggaatacat
     3841 ttaaaggttg tgacaaaatt cgtgtcacaa tcttttttta ttttgaataa gtgatgttca
     3901 aaaaccattt tctcggtatt aagccaaatt tatgcaaaaa tgtgagaaac tattttcaca
     3961 aaatttttct tcatactcga aactaatcgg cttcttcacg acctcttata cacggcgttc
     4021 aaaagcttgc atctgcgata ataaccccaa ccaaacgaaa aacatgagaa gctgtttatg
     4081 tttggctgtt cttattactt gatgcaggac agctttttta cgacctcttg gtacagggta
     4141 gcttttttac caatttctag ctataattaa agataacctt gaaagttgca gaaaaaaagt
     4201 gcaaaataaa cgataagtac aaaaagacta gttgaaagga cgtcgtcatg tcagagcgtg
     4261 gattattaat cgtgttgtct ggtccatccg gtgtaggaaa aggcacagta cgaaaagcaa
     4321 tttttgagag tgaggataat gattttcagt attcgatctc aatgactacc cgtaaaatgc
     4381 gtgaagggga ggtcgaaggc gttgattatt tcttccgaag caaagaagag tttgaatcaa
     4441 tgatcgaagc tggagagatg ctggaatacg cagaatatgt cgggaattat tatggaactc
     4501 ctttgtcata tgtcaacaag acattggatg aaggaaaaga tgttttcttg gagattgaag
     4561 tccaaggagc aaaacaagtc aaagaaaaag tgcccgatgg tgtattcatc tttttgactc
     4621 cgccagattt agcggaatta aaatcacgga tcgtaggccg aggaacagat gcagatgaag
     4681 taatcgaaaa acgaatgaaa gttgcaaaag aagaaatcga aatgatggct ctttacgact
     4741 atgctgttgt aaatgatgaa gtgccgctag cagttgaacg aatcaaaaac attattgcca
     4801 gtgaacattt tcgagtagat cgcgtaatcg gtaaatatat taaaatgtta aaggagatgt
     4861 agtcttatga tgttaaaacc ttcaattgac tcactattag atcgtgtaaa ttcaaaatat
     4921 tctctagtga ttttagcaag taaacgcgcg catgaattgg atgcaagtgc acagcctact
     4981 ctagattctt ttgattcagt aaaaagcgtc ggtcaagctt tagaagaaat cgatgcagga
     5041 aatgtagtca atgatccaca tcctgaatta aaacgtgaac gtttgaagat ggaagaagag
     5101 gaacgtcaag cacaaaaaga gcgtgaacaa cacgaattgg aatcacgtat ccgtgaagag
     5161 caaaaaatgt aataaagaaa aatagagcga acatctaaaa agaaaagcaa acccagctgt
     5221 ttccttttca aacattcgcc acttattgtc tggtagaaaa ggctgtgtct gaactttttg
     5281 acacagttct tttttttctt atctgctgtt aattttttgt taggatttgg cagaagataa
     5341 gacaaatcca tcgatctcta gtaaaatagg caaggaactt tttgtcgctt gataagaaaa
     5401 gacaaaaaaa ggaggaaacg aaccatgaca ttaacagctg aagtcattgt ggacgtacct
     5461 actatgcaga ccgatcagcc attcacttat ttggttccct ctgaaatgga aacagctttg
     5521 caagtcggga tgcgagtaga agtccctttt ggtaatggca atcgtcatgt ccaaggattt
     5581 gttatggcta tccaaaaatc agaagaatca gctaatccat ctttgaaggc agttattcgt
     5641 ttgctagacc ttgctccggt ggtcaatgaa gaattacttt cattagctga ttatatgaaa
     5701 aaaattactt acgcattcaa aatcacttgt ctacaaacga tgcttccgag tgtgatgaaa
     5761 gcagaatatg ataaattgat ctatcctttg gcagacacac cagaagttca ggtattgttt
     5821 gatcagcgag aagtgatttc ctggaaggaa gcagaagaag aaggcagcct ttctcaattg
     5881 attcgctggc gccaagagca attagtagat atcaaatatg aagtccatac acgaaataaa
     5941 gtaaaaacga ttcgtttggt ccgttctttg ttaactgaaa agcaaatcga agaagaatgg
     6001 gcaaaattac ggcaaaatgc caagaaacaa aaagaacttc ttttgtgttt atctgaactg
     6061 agccaagaag aaccaattgc ttatttcaaa aataaaggaa tcagtactgc tgtcttgaat
     6121 caaggaaaag aaaagggctg gctggagttt atagaatcag aacgttatcg cgacccttat
     6181 aaagaccgag tatttgacca aacgacggca ttggaactga atgcagaaca aaaaaatgct
     6241 gtcgaacaaa tcatcacagc aggtcagcag caaaaagatc aggtcttttt gctggaggga
     6301 atcactggca gcgggaaaac agaagtatat cttcaggcaa tcgctgatgt tttgtcagaa
     6361 aataaaacgg ctattatgtt agtgcctgaa atcgcattga ctccgcaaat ggtcgagcga
     6421 ttcaaaggac gcttcggaga atcagttgcg gtcttacata gcggactttc ccaaggtgaa
     6481 aaatatgatg aatggcgcaa gattgagcgg gaagaagcgc aagtagtagt aggcgcacga
     6541 tctgctattt ttgccccgct gaaaaatatc gggctgatca ttatcgatga agaacatgaa
     6601 tcaagttaca aacaagatga gacaccgcgt tatcatgcga gagatctagc aatttggcgc
     6661 agtaaatatc atcattgccc aatagttcta ggaagcgcga cgccctcttt agaatcaaga
     6721 gcacgagctc aaaaaggcgt ttatcagtta ttgcagttaa accaccgagc caaagcagct
     6781 gcacagcttc cagctattga gatcgtagat atgcgtgaag agtttcagaa tcatcggacc
     6841 tctacttttt ctgctaattt gcaggaaaaa atacaaaatc gattagacaa aaaagaacaa
     6901 actgtcctac tgctcaatcg tcgaggatat tcgtcttttg tgatgtgtag ggactgtgga
     6961 tttgttcttc catgtcctaa ttgcgatatc tctcttacac tgcatatgga tacacgttca
     7021 atgcgctgcc attattgcgg acatgaagaa cctattccca atcgctgtcc taattgtggc
     7081 ggcaataaaa tccgctacta tggaacaggt actcaaaaag tagaagagga actccgagaa
     7141 ctttatccgc aagctaggat tttgcgtatg gatgtagata ccactcgaag aaaaggggca
     7201 cacgaacaga ttttacaaaa attcggtgca aaagaagcag atatattatt agggacacaa
     7261 atgattgcta aaggtttgga ctttccagaa gtgacattag ttggggtgtt gaatgctgat
     7321 acctccctga atttaccgga ttttcgttct agtgagcata cattccaatt gcttacccaa
     7381 gtttcaggac gagcagggcg agcggaaaaa gcaggcgaag tcgtgatcca aacattcaat
     7441 cctcaacatt acgccataga attggctaaa aaacaaaatt atgagcagtt ttatcagcag
     7501 gaaatgcatg tacggcatcg tggaggctat cctccttatt actttactgt gaaaataacc
     7561 gccagccatc ctgaagaaca agtagcggca aagaaaatct ttcagattgc caatcagcta
     7621 aaagaagtgt taagccctca aagcttgcta ttagggccaa cgcccagcat gattttgaga
     7681 gtgaaaaacc gatattatta tcaactaatc atcaaatata aacatgaacc gaatcttcct
     7741 catgttttgg atgaaatttt gaatggaagc cagaaagaac agcgacaagg tctttttgtg
     7801 gcaattgaca acgaaccatt aaactttatt taagaaaggg ccaaattatg cgctatccta
     7861 tattgatcca ccctaatgat aaattaaaaa gaactgctca gccaatcgat gtaatcacgg
     7921 acgagacaat cgctttatta gacaatttat atgagacaat gattgcgaat gacggaatag
     7981 ggatcgctgc tcctcaagta ggacaaaaca agagaattgc tgtgatcgaa gtagatgaag
     8041 gcgagaaatt tgaactgatt aacccagaaa tcatcgaagc aaaaggtgaa agccttgacg
     8101 tagaaggttg tctcagtatt ccgcatgtct atggaacggt caagcgagca gatgaagtga
     8161 ccgtgcgtta ttatgatcga gacggagaag agatagaagt gacggctttt ggctatttag
     8221 cccgtgcctt ccagcatgaa atcgatcact tagacggtat cttgttcatt gagaaaatga
     8281 tacagcagat cccggaagaa gaacttgaag aatatatgga ggaacatctt gatgactaag
     8341 ctagtattta tgggcacacc tgctttttct gtgccgattc ttgaagggct gatagaatca
     8401 ggttacgaga tccaggcagt ggtaacacag cccgaccgtc cagttgggcg gaaacgagta
     8461 attacgccga caccggtaaa aaaagcggct ctgaagcacg gtatccgtgt gctgcagcca
     8521 gagaaaatct ccggttcgcc agaaatggaa gaaatcattg aattggtgcc tgatgtgatc
     8581 gtcactgcgg catttggcca attcttgcct gaaaagttat tgcaagtacc aaaattaggg
     8641 gcaatcaatg tccatgcttc tttacttcct aaatatcggg gaggtgcgcc tgttcattat
     8701 gccatcatga atggggagaa agaaactggt gtaacgatca tggaaatgat caaaaaaatg
     8761 gatgcaggcg gaatctatgc gcaagaaagc ataccaatca ctaaacaaga tgatgtaggg
     8821 acgatgtttg aaaagcttag ccttttagga agaaagctat tgctagaaac attgcccaat
     8881 attttggatg gacaaaaacc agtacctcaa gacgagtcag aagcaacttt ttcgccaaac
     8941 atcacacggg aacaagaagc aatcgattgg cataaaactg cagaagaaat tgacaaccaa
     9001 gtacgcggaa tgcgtccttg gccaattgca ttcacgacct atgagggcat acgctggaaa
     9061 ttattatcgg ttgaagtatt ggcagaaact acagacaaag cacctggtac gatcatccgg
     9121 aaagacaaaa aaaatctatg gattgcttgc ggtaaacaga ccgtcttagc catcaatgaa
     9181 ttacagccag ctggtaaagg aaaacaggcc gtacatgatt tccttaacgg aagcggtcag
     9241 caagttcaga ttggtcagca ggtgaaataa tgaaaaaaat ccctaaaaaa atgaaaaaat
     9301 cagtacgata tatggcatta ctgacattag agcgagtgaa caagggtggc gcctactcga
     9361 atcttttgtt gaatgagatg atcaaacaaa gcgagctaaa tcaaaaagat gtgggtttat
     9421 ttacagaatt gatctatggt acgattagcc atcagcgact attggaattt tacgttgcac
     9481 cattgatcgc taaagcaaaa aaagtggatg actgggtcaa gatgcttctt tatctttctg
     9541 tataccaatt agaattttta gataaagtcc ctgcacatgc tattttaaac gaggcggtcg
     9601 aaatcgcaaa agtaaaagga aatcctggga cagggaaatt tgtaaatggt gtcttgagga
     9661 attatcagcg tcaaggagca ccagatctgc aaggcattgc tgatcctatc gaaagacttt
     9721 ctgtcaaaat cagcctgcct ctatggatga cgaaacgact ggtggatcaa atcggatacg
     9781 aagaaactga aaagctcggt ttatcgctat atcagcctag ccatgctagt gcccgagtag
     9841 atacacgccg actttccaga gatcaggcca tcactgtttt gcaagaagaa gagatcacag
     9901 ctgaaaaaag ccaagtcagt ccatacggta tagtagcgaa aaaagggcat ttagcttcta
     9961 gctcattgtt ccacgatggc gtcatgacga tccaagatga aagttcgatg cttgtagcgc
    10021 cgtctatgca aatcgagcct gaacatcatg tcttagatgc ctgtgcagca cccggaggta
    10081 agacgacgca tattgcttct tttttagatc cgtcatgcgg tggagaagtg gtcgctttag
    10141 atgtacacga acacaaagtc aaactcattg aagataatgc agcacgtcta ggtgtctctt
    10201 ctgctgtcta cgctcaaaag atggatgcta gagaagtcag taaacagttc gaagcagagc
    10261 agtttgatcg gattttagta gacgcgcctt gttcaggtct tgggctgctt cggcgaaaac
    10321 cagatattcg ttataaaaaa cagccggatg aattagcgaa tttaccaaaa atccaattgg
    10381 aaattttaga aagctgcgca ccaactttaa agtcttccgg tattctgact tatagcactt
    10441 gtacgatctt aaaagaagaa aaccaagacg ttgtttctgc tttcttggaa agacaccctg
    10501 attttgagag gattgatgtt ttgaccaatc ctatgctaca atcatcaatc gaagagaaaa
    10561 tgttgacttt gtatccgcat cagttttaca cagatggatt tttcatttgt tgtttgcgca
    10621 aaaagtaatg aggtgaaaaa atgcagattg aatatcaatc agatgtagga agacgacgta
    10681 acacaaatca agactatgcc agtgtattta ccagccgttc aggtctcaag cttgctgtat
    10741 tagcagacgg gatgggtggt catcgtgccg gagacattgc cagtcagatg gcggtaacga
    10801 atctaggcga agcttgggaa aaacaagaag ctgttgacga tgaaaaaatc gcccaatggt
    10861 tcattcaatc gattcaagaa gaaaatatgc tgatttatca gcgtggacaa gaacagccgg
    10921 aatataacgg tatgggaaca acaatcgtag cggccgctct gtcagatgac tggttcacga
    10981 ttgccaatgt tggggatagc agagcttatt tgattcgtga agataaaatc atgcaattga
    11041 cggaggatca ttctttagtc aatgaactag tcaaatcagg agaaatcagc gaagaaatgg
    11101 cagtgaacca tccaaggaaa aatattttaa cacgttctgt cggcatgcct ggaacagtcg
    11161 aagtagatgt ttctactcat ctttggcaac taaaagatcg actgctgtta tgttcagatg
    11221 gcttgacgaa catgctgtct gaagaagaaa taggaacgat catcaatcaa gaaggcccat
    11281 tgtctcataa agtaactgag ctgattgatc gtgccaatga agcaggcgga gcggataata
    11341 tcaccgttct tcttatcgaa tataaggagg acagtgtatg atcgagttgg gcaaaaaatt
    11401 aaatggccgt tatcaaatca ctggaaatat cggtagcggt ggtatggcaa atgtattttt
    11461 ggcgcacgat ttaatcttgg atcgtgatgt agcagttaaa gtcttacgat ttgactttca
    11521 aaatgaccag actgcgattc gacgatttca gagagaagct ttagcagcaa cagaactcgt
    11581 tcatccaaac attgttagtg tttacgatgt tggtgaagaa gacaacatgc agtatctggt
    11641 gatggaatac gtcaaaggaa tggacttgaa acgatatatc caaacacatt atcctgttcc
    11701 ttatgaaaca gcagtaaata ttatgcagca gatcctttct gctatttctt tagctcataa
    11761 ccatcaaatc atccaccgag atttgaaacc ccaaaacgta ctgatcgata atgaaggtgt
    11821 tgtaaaaatc acggatttcg ggattgctat cgctttatct gaaacatcga tcacgcagac
    11881 gaatacgatg ctaggatctg ttcattatct gtcccctgag caggccagag gaagtatggc
    11941 aacgaagcag tcagatattt atgctttagg gatcatcctt tatgagatgt tgacgggtag
    12001 cgtaccgttt gatggtgaat ctgctgtaac aattgcttta aaacattttc aagatgatct
    12061 tccatctata aaagcacttg atccaaatgt gccacaagca ctggaaaacg tgattttacg
    12121 ggcaacggct aaagaacctg cagaccgtta taagtcagcc gaggaaatgt ctgatgatct
    12181 ttctacagta ttatcaccag ctcgtgcaaa cgaagaaaaa tggcagcctc atgtgatgga
    12241 taatgaaaca aaagttatca caccccttac ggaagatgca ccaatgcctg attcattcaa
    12301 atctatgcct ctgccaaaag acaagccaga aaacgaaacc ttaccagagg aggaagaagt
    12361 tctgcctgat cctaaaaaga aaggacagaa aaaatggtgg ctgatcctat tagctattct
    12421 tgctgtttta ggaatcggaa cagctgtatt ctttgcttct ggcgggcgag gagaagttgt
    12481 tgttcctgat gtttctgatc tttctgaagc ttctgctaga gaaacactcg caaaagcgaa
    12541 attagaagta gcaacaaaaa cagaagaaat tgcagatgac aaaatcgaag aaggtaatgt
    12601 agtgaagaca gacccggcag cgggaacgac agtcaagcaa aaacgcgaag tcactctgta
    12661 tattagtagc ggcacgaaaa aaatcaaatt agatgattac agtggtaaaa gttatgaaga
    12721 tgccgaagca agtttgaaag aattagggtt ttctgctgac ttgattcaaa aagaagagga
    12781 attcagttca gaggtagaaa gcggaatgat catttcgcaa tctccatcag aagatacaga
    12841 agtcgatcct aaaacagaca cgattacttt cacagtcagc aaaggaccag aaacagtgac
    12901 tttgagtagt catgtaggag aaaactacga aactgttcgt aattggcttg ttacacaagg
    12961 attcaatatc aataatatta gtgctactta tgactatagc gacactgtac catcaggttc
    13021 gatcatcagt caggatccag cctcgggtga agttgtagca gaagaaactt acatcaattt
    13081 tgtcgtaagc caaggaacag agcctaaatt gaatgatatc tccggctata caaaaagcga
    13141 agcacaaagt tatttagcta gtgtcggtgc agaatatatc gggcatgaga cttccgaata
    13201 ttcaaacacg gtggataaag ataaagtgat tcgtacagta ccaggggcag gaacgacaat
    13261 taccaaagga atggttgtga acgtaattta ctctaaagga ccagatccag cgtcttctag
    13321 ttctgaaaaa gactcttcag acagttctag cagctcaagt agctccagta gcacaagcaa
    13381 ctcttcaaaa gaagaatctt ccagctccac aagttcatca gctagcacct cagaaagttc
    13441 aaaataagtg ctgaataaaa tatatcagaa gggaatgcga cattgatttg ttgcattctc
    13501 ttttttgtgt ttctatctct tttgatcaat tttcaagcca tatactttga gaagtttgca
    13561 tggaataaca cctactttcc tcttcgtatg gtaaaataaa caaaaacgga ggtggcactt
    13621 atcaaaggac aaatcagaaa agcaatcagc ggtttttact atgtatacgc aaacggagag
    13681 acctatcaaa caagaggaag aggaaatttc cggaatcgga aaatcacgcc gcttgtcgga
    13741 gatcaggtga tctttgagag tgaaaatcaa acagacggct atcttctgga aatcttgccg
    13801 agaaaaaatc aattagttcg cccaccagtc acaaatgttg atcttggaat cattgtcaca
    13861 agtatcgtag agcctgcatt ttcttataat ctacttgacc gctttctagt taccttggaa
    13921 tacgagtcga tcgaaccgat tatttttttg acaaagaccg acttgtcaga cgacccagaa
    13981 aaaatcgaag agatcaagca aacctatact aaaataggct atccggtgat tgtacccaat
    14041 catacgggtg atacgacaga actgatccgt tacttcccag aacggctatc ggtctttatg
    14101 ggtcagtctg gagcaggaaa atcgacgcta ctgaatcaaa tatcaccgga actgaattta
    14161 gcaacaggag agatttctga tgcacttgga agaggacggc atacaacacg tcatgtggaa
    14221 ctgattccta tctatggcgg gttagtagca gatacaccag gatttagctc gattgacttt
    14281 ttgacaattg aagcaggaga actgccgaga caatttcctg aatttgtaga agcttctgtc
    14341 cattgcaaat ttagagagtg catgcatgct aaagaacctg gctgcgaagt aaaacataga
    14401 gtagaaacag gtggtatcgc acagacacgt tacgacaatt atttgcaatt cttacaagaa
    14461 atcgaaaaaa gaaaaccaat gtatgcaaaa aaagaaggta aacgaaaata ggaggaaaca
    14521 agaatgaaga tagcaccatc cattttgagt gcagattttg ccaatttaca aagagacatt
    14581 gagttagtgg aaaaaggagg cgcagattac atccacgtag atgtaatgga tggccgattt
    14641 gtcccaaata tcacattagg gccaaatatt gttcaggcaa tccgtcctgt tactaaatta
    14701 ccgttagacg tccatctgat gatcgttgat ccagaaaact acattccagc atttgcaaaa
    14761 gctggagcag atatcattac tgtccacgtc gaagcaactc ctcatattca tcgggcactt
    14821 caaatgacaa aaagcttagg agttaaagct ggagtagtta tcaatccagg aacaccagtc
    14881 tccatgatca aacatgtttt gcctttggct gatcaagtac tagtcatgac tgtcaatcct
    14941 gggttcggcg gacaaagctt tattgaagaa acagtagaaa aaattacgga actcgctgat
    15001 ctacgtagag agaacaattg gacttatgca atcgaagtag atggcggaat cgttccagaa
    15061 acagcaaaaa tctgtaaaga tgcaggggcg gatgtcttcg ttgcaggatc atatatctat
    15121 ggagccgaaa atccagtcgg acaaatcgaa cgattaaaag aggcattaga ctaaatgaaa
    15181 attttattag ctgctggagg taatccgcaa aactggccgc agtctctgct gtccaaatac
    15241 gatgcctatg tcgggataga ccgtggtgcg ctgtacctgc ttcaagcagg ctacccagta
    15301 gatctggcag tgggtgattt tgattcattg tcgaacgaag agcagaagga agtatttgca
    15361 aaagccaaag agtatacccg ttcccctgca gaaaaagacg atacagatac acagttaggt
    15421 atcttaaaaa catttgaacg ttacccgcaa gcggaagtca ctttgattgg agctacgggc
    15481 ggaagattgg atcatctttt agccaatctc tggttaggta tggaacctcg cttcaaaccc
    15541 tttgcccatc agcttattat cagagattgt caaaattatt tgtcttatta tttgcctgga
    15601 gagcatatag taagacagat accagatatg aagtatttag cttattgctg cttgactccc
    15661 gtctcgaatc ttacattaaa aggcagcaag tatgtattgg aaaataaaga agtagaatat
    15721 ccgacatctt atgcaagtaa cgaatttctt tcagataccg cagaattttc tttttcagat
    15781 ggtatgattg cagttattca aagcaaagat tgatccataa acaaataaaa aaatttgtct
    15841 ttcaggctaa aaatgacaga atctattatt tgctaaaaaa aagcttaaga ggagcaactt
    15901 accttttgtt tcacggcatt tagtgataaa attgaacaca agagtctaat taattcgagg
    15961 tgtctttgtg aagaagttca atcctaataa aaatattatt attaccttgg ttattgttat
    16021 catcgtggtg acgatactaa gtgtgaccgc tgcgcagcga gcgaatgaag gaaagcccaa
    16081 tttcttccaa tccatcgtta atgacagtgt tgcaatggtt gataaaacaa tctctgcccc
    16141 agtacggtgg gtacgaaatg gagtagattc tgtccataat cttttttcaa cttatgccga
    16201 aaatgagcgg ctaaaagaaa aaatcgatag ctatgatgac gtttcacagc aaaacaagaa
    16261 ttacgagaga gaaatcgaag cgttgaaaaa agaacttgag ttgaatcaga cgctgactag
    16321 ttacgaaaaa gtaacggcaa atgtcattac tcgttcgcct gacagttggc aggacatgct
    16381 gatcgtagac aagggttcta aagacggtat cgaagcaaat atggcagtca tgtcgcaaaa
    16441 aggattgatt ggccgagtga tcgaggtcaa tacggcttcg tctaaaatcg aattactgtc
    16501 atcctctaat gaaagctcca atcattttcc agtacgggta tcttcggcta atggcgaagc
    16561 gtttggtttg cttaaaaact atgatgaaaa gctccatgcc ttagtggtga cccaattaac
    16621 tggtgatacg gatatcaaag aaggggatgt tgtccagaca tccggtcttg gagggaattc
    16681 tccagctaac ttgccgatcg gtacggttat taaaacgaaa ccagatagtt atgggctgga
    16741 tcgggaagtt tatgtgaaac cttatgcaga aatgtatgac gtgtcagttg tgacgattgt
    16801 ccaacggcta gcggaggatt agtccatgtt gaagaaagaa acaatgaagt actatctgcc
    16861 aatcgttttg ttctttttga tgttgataga tggtcattta acaagaatgc taggggagtg
    16921 gtcgaaaggc acctatatgt caaatgccca ctttctgata ttggcattat tatgttgcag
    16981 tatggcgttt gaaaaacgtt atttactgat taccacgatt gttctcgggg ctatctatga
    17041 cgcttactat attggcgtta tcggtatcta tgcagtagct ctccctttaa ttgtatggtt
    17101 gatgtatgta atgaaagacg ttatccatgt caacatcttt actgaatttt tcagtatgat
    17161 catctttgtc acgggttatg aattgtttac gatggtggtc cagttgattt ttaaattagc
    17221 agtagtaaat aacacgtatt ttattacaag gtttttagga cctacactgc tgttgaacat
    17281 gattatattt gtattattca tttttccctt taagaaatta ttcagtgatg aataatttct
    17341 ttttttattt ttaggtcgaa agcgaaaaag aatcgttgtt atcttaagaa atgctagact
    17401 taacgcggct ttttcatgat cattttctga aacgaacagt tttgtcattt ttaaaagttg
    17461 ttttgaaaca tttttgtaac ataactatta tatcgttgac acactgctat ggtacaattt
    17521 ttatatcgtt aagaaagact taaatatatt tagatattta ttaaattaag ccagtcggag
    17581 gaatgaagag tgaaaaagag tttaatatca gcagtaatgg tatgttcaat gaccttgact
    17641 gccgtagcat caccgatcgc tgccgcggcg gacgattttg attctcagat acaacagcaa
    17701 gatcaaaaga ttgcagactt gaaaaatcaa caagcagatg cacaatcaca aatcgatgcc
    17761 ttagaaagcc aagtttctga aatcaacaca caagcacaag acctattagc aaaacaagat
    17821 acattgcgtc aagaatctgc acaattagta aaagacattg cagatttaca agaacgtatt
    17881 gaaaaacgtg aagatacaat ccaaaaacaa gcacgcgaag cacaagtaag caacacaagc
    17941 tcaaactaca tcgatgctgt attgaatgct gattcattag cagatgctat tggacgcgtt
    18001 caagcaatga caacgatggt aaaagcaaac aacgacttga tggaacaaca aaaacaagat
    18061 aaaaaagctg tagaagacaa aaaagctgaa aacgatgcta aactaaaaga attagctgaa
    18121 aaccaagctg ctttggaatc tcaaaaaggc gatcttttat ctaaacaagc tgatttgaac
    18181 gtactgaaaa cttcattagc tgctgaacaa gcaactgctg aagacaagaa agctgacttg
    18241 aaccgtcaaa aagcagaagc tgaagcagag caagctcgta ttcgtgaaca acaacgtcta
    18301 gcagaacaag ctcgtcaaca agctgcacaa gaaaaagctg aaaaagaagc acgcgaacaa
    18361 gcagaggctg aagcacaagc tactcaagct tcatctactg cacaaagctc agcaacagaa
    18421 gaatcatcag ctacacaatc ttcaatgact gaagaatcat catcagctac acaatcttca
    18481 gcaacagaag aatcaacaac acctgaatct tcaacagaag aatcaacagc gcctgaatct
    18541 tcagcaacag aagaatcaac aacag
//
LOCUS       DAZKMK010000057        16558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000057 DAZKMK010000000
VERSION     DAZKMK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.057"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG8V1_001815"
     CDS             14..1141
                     /locus_tag="ACG8V1_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0329908.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG8V1_001816"
     CDS             complement(1359..2501)
                     /locus_tag="ACG8V1_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0329909.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACG8V1_001817"
     CDS             complement(2506..2691)
                     /locus_tag="ACG8V1_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0329910.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="ACG8V1_001818"
     CDS             complement(3045..3290)
                     /locus_tag="ACG8V1_001818"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329911.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="ACG8V1_001819"
     CDS             complement(3284..3685)
                     /locus_tag="ACG8V1_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0329912.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACG8V1_001820"
     CDS             complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACG8V1_001820"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0329913.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5703..7469)
                     /locus_tag="ACG8V1_001821"
     CDS             complement(5703..7469)
                     /locus_tag="ACG8V1_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0329914.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7482..8276)
                     /locus_tag="ACG8V1_001822"
     CDS             complement(7482..8276)
                     /locus_tag="ACG8V1_001822"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0329915.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8286..9662)
                     /locus_tag="ACG8V1_001823"
     CDS             complement(8286..9662)
                     /locus_tag="ACG8V1_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0329916.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9782..10429)
                     /locus_tag="ACG8V1_001824"
     CDS             complement(9782..10429)
                     /locus_tag="ACG8V1_001824"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0329917.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="ACG8V1_001825"
     CDS             complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="ACG8V1_001825"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0329918.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="ACG8V1_001826"
     CDS             complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="ACG8V1_001826"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0329919.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13096..14727)
                     /locus_tag="ACG8V1_001827"
     CDS             complement(13096..14727)
                     /locus_tag="ACG8V1_001827"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0329920.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            15031..15702
                     /locus_tag="ACG8V1_001828"
     CDS             15031..15702
                     /locus_tag="ACG8V1_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0329921.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(15862..16053)
                     /locus_tag="ACG8V1_001829"
                     /pseudo
     CDS             complement(15862..16053)
                     /locus_tag="ACG8V1_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         5370 a         3200 c         2222 g         5766 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
     3901 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
     3961 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
     4021 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
     4081 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     4141 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     4201 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     4261 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     4321 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     4381 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     4441 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     4501 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     4561 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     4621 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     4681 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     4741 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     4801 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     4861 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     4921 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     4981 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     5041 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     5101 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     5161 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     5221 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     5281 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     5341 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     5401 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     5461 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     5521 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     5581 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     5641 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     5701 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     5761 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     5821 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     5881 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     5941 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     6001 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     6061 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     6121 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     6181 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     6241 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     6301 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     6361 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     6421 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     6481 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     6541 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     6601 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     6661 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     6721 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     6781 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     6841 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     6901 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     6961 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     7021 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     7081 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     7141 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     7201 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     7261 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     7321 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     7381 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt
     7441 ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt
     7501 ttccaaaaag tatctatata tttttgttca atatctacat aatctcccga taaacattta
     7561 aataaaacat cagttcttaa gttttcccat gattcttttt ctttattgac caaaatagga
     7621 taaaagaaaa ctttattttt atttgcagct tctagatcac caggagaatc accaatcatt
     7681 aatatcttat ccaaatcaca accattttca attacacttc gaataatatc ttcttttttt
     7741 cctttatctt gacaacacag ttcatcaact aatgataata attcatgtct ttcccattct
     7801 tcttgaacag cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga
     7861 gcttttatag tatctctagc acttaaaaag ggtttatcta atccttttgc ttgactaatc
     7921 tgttgattga cttcttccga ccattttaaa gcttttaata aatcctctga ctgagaatat
     7981 tctatttccc tcttcaaaga tggattagac aattcttttg ttagagatac ccagtgcttt
     8041 aatcgattaa tcgatgttac atcatttttt tctaatgttt taactaaacc taaaaatctg
     8101 tttattccac gagtctctga atataaatta atattattcc aatcctgtag gaatttttct
     8161 ctatgatgaa catcaaaaat ctctgcagca attggtccga aaaagagatt gtgtttataa
     8221 gtcatcgtat ccataacaca gccatctgaa tcaatacaaa atatccaatt attcatattt
     8281 ctctcctatt tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt atctttagat
     8341 aatggataaa ggaaaaataa aattaatcct acaatcagaa aacaaattga tggaattaat
     8401 gttgaagcgt tatataactt ttctaaaaca tctcccgttt gtacatttgc taaagagtca
     8461 tagcctataa tagaaagagt ccaacctccg acgcctccag ctagagcttg acctattttt
     8521 ctagcaaaag aatataccgc atatacggtc ccatcttctc tttgatcaga ctttacttct
     8581 tgatcatcaa taacatcagt aatatttgcc caaataacag tattaaacac acccaaaccc
     8641 atatagccaa ccgaactaag aattaagaat acatacatat tactagcttt tatcaaaaat
     8701 aagatcaaat atactattgc tgaaaagaac attcctactg atgccagttc tttttttcca
     8761 tattttttac ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa aataggattg
     8821 ataaaattca ttaaaactat tccctgtgca tcattatagt aatcaggaaa tacataatta
     8881 ttcatactca taattaataa ttgagcaagg agtaagaaaa tagccgcacc aattatagaa
     8941 agtaatgccc tatttttaac taaccctttt aaagatttta acaatgaaac tttgtattga
     9001 gattggtcaa ttcttactcg ttcagtagtt agtttataac atattaaata acaaataagt
     9061 gcacatatcg aaaatattcc cgcaacaatt gtaaaagttg atccaccacg aactatttga
     9121 gcgcctgaat catctcttgt ataaataaat aatggtgtta atacaccaat gaccaaactt
     9181 gctaaagtag ctccaacatt tcggaatgtc gatagcattg ttctttcttc tgtaatagaa
     9241 gtaatagctg atgccattga tccataagga atatttatag atgtgtaaaa aatactcccc
     9301 cataatatat acgtaacata catgtatact accttaacgc tcatagaagc tgaaatcata
     9361 gaagtttgat acattaaaaa acttgctaaa gcgactggac cactcattct taaaatccac
     9421 ggtttaaact taccctgctt agttgctttc gatctatcaa caatacgccc catagttaca
     9481 tcagtaactg catcgataaa tcttgctact aaaaataatg ttccaacggc agctccgctt
     9541 atccccaata cttttgtata aaagatcatt aaaaaagaac tagcaaaaat gaaagtaaaa
     9601 tcgtttccaa aatctccaaa catatatcct attttgtctc ttataccaaa ttttctttcc
     9661 atacaattct ctcccttctg ttgttaagcg ttttcattct gatgaaagta cgtactatac
     9721 tctgaagcgt aaagagtcag tatttatttt ttacatttca gagtattatt tgttttttct
     9781 cctaataatt ctttatctca taaaacttat ctatatattt actagctact cgacttactt
     9841 cttcaaaatc acctttttca acagaagcca ataaattccc acctacacca actgcaacaa
     9901 ctcctacttc aaaccaatct tctaagttct ctatacttac tccgccagta ggcattatat
     9961 ttacgtgtgg taatggagct tgataggctt ttacaatgga tggaccaaaa gcagaacctg
    10021 ggaatagttt aatgatatct acgccagcag taagtgcctc ctttatctca gtaattgtca
    10081 tacaacctgg taaataagga atttgatata aattacaaat ctcagctgtt tgtcgatcaa
    10141 aagatggact aacgatatat tctgccccag ccataattgc gatacgagca gtaaacgcat
    10201 ctaaaaccgt accggctccg attattacat ctttttgatc agcatatttt ttagcaattt
    10261 ctttgattat ttcttcagca ttaggaactg taaaagttag ttcaatccct ttaattccac
    10321 ccgcaataat tgaatttatt gctttaatgg cctcatcctt atttttccca cgtacaaccg
    10381 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    10441 tcctttatgg atacatactt tgtctagctg tactaactta caaagaataa atttaagctt
    10501 ttatttcaag tattattttc tacttccgat ttcaaaaaaa ttaaaataat tatacgcatt
    10561 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcggcc
    10621 tgctttaacc cactcgctta gtaattgaca taatactcga cgaaaatact catggcgagg
    10681 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    10741 ttgtgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    10801 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    10861 tggaagccaa tcagttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    10921 ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    10981 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    11041 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    11101 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    11161 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    11221 accgtgatct gctaatcgac agccaatttg atgaaaatga tccactcgtt ggctcaatgc
    11281 ttgcttcaac ccattaaagt ctttgattgg tatagccgct gtttgactaa gttttaaaat
    11341 atagttcgaa aagcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    11401 taccttgaaa gaattatcct gtttagaaag ctcagtatga taccacaagt catctgtagg
    11461 atcatccgtt gtacagatgg ctttcacatc catcatctcc aacaattctt tcgggcgata
    11521 ggaatccgtt tgaatttttt cgttagcacg tcgccaaatt tctggtgcat tcttctcaga
    11581 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    11641 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcatcacc
    11701 tggaccactg atcaactctt cttctatacc attagctcgc atcaatcgcc atttataatg
    11761 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    11821 tagcaaatat gctgggtcta aatgacaatg atagtcgata attggttggt gttttgcaaa
    11881 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    11941 atttaaacac tcctttcctt ccgttagttt ttagaagaga aaatttttcc tctaaataaa
    12001 ctcccttata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    12061 aaactgcacc tagagctcgg tcatataatc cataaccagg tctaccggtt tctccccaaa
    12121 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    12181 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttcg taaaaatcga
    12241 gtccattgtc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    12301 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    12361 tacataatgt aataccatta gaaggagaat ccactacatt acacaaccaa tctaaatcgt
    12421 cacgattttt aacaatacga ggtaatccga aaatggaata tggtggatca tctgggtgaa
    12481 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    12541 agttctctcg taatttctct tcatcaactt ctttataggc ttctaataat tgcttaattt
    12601 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    12661 ctctaataat ttcctccgga tcagtaggga tattttttga aatataggct aacgtagaag
    12721 acccatcatc taaacgataa tccaaatccg attttaccca atcaaatact ggcataaaat
    12781 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    12841 caatgtattg atctcgtgtt ggtaaaccaa ttttgatatc atcatgaaca ttaacactct
    12901 caataacttt caaggtaagt ccattggctt cgacttcatc ttttaaagat ttaattcgtt
    12961 ctctcggcca aacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    13021 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    13081 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    13141 aactgcttga ggacctacta gcatttcgca aaagattttt tcagttttct ttcctacccc
    13201 aacttgatat aaatctaaac caaagatttc tttattttct aaaatttcct gtaccgcaca
    13261 atgaatcttt gaaggatctt gttcacctaa tttcaatact gataatttat cctgcaactc
    13321 taccaataac ggatcaggac ttggcacaaa attttcccct tgatcatcga ctgccagtaa
    13381 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    13441 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    13501 tgttgcaatt cgttgtggtg tatctggaat atagggatta ggcaaacgtt tattaattac
    13561 ttcatcaata aattgttttg gatcaataat gcctggattt tcaactactg gtaaaccttc
    13621 taaataacct attcctttga ttaaaccaac caaatcatca tcctgcatct ctgctgaaat
    13681 tgattgatat cctagcagac aacctgttac tgccaaagca gtatgaagtg gattcaagct
    13741 tgtcgtaacc ttcatgatgt ctgctttatc aaccgtctct ctatctgtca taatcacacc
    13801 agccttttcc aatttaggcc ggccatttgg aaaactatct tcaataacta aataatgaac
    13861 tacctcagta ttagtgaaag cagcaatatt ggtatgtttt tttgtgtgaa tgatatctaa
    13921 atcctcgagc cccatcatca atagatcttc ctttacattc tctgaaggat ttggcgtaat
    13981 tcgatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    14041 tttttcagaa acatatcctt ctttttgcca agcttctgca atcgtaaaaa tactatcttg
    14101 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gcaactctcc
    14161 agcattaaat ctgctatata acaaggatgt tacaatactc atcgtatgtt gaggattgct
    14221 aggtccagat ttgatatcag ctttgactac cgaaaagaat tgccctttag aatcttttaa
    14281 actgtaacct ttttcagtaa tagtaaacgt tgtaaactgt aatttaggat cttcaaaata
    14341 tttaaccact ctttgatagt ccgctatatt cttcggattg caatataaac tatcagccgt
    14401 tactgccaaa agtctttttt tgagttcacc atctccactc ataacaattt ccaaaatgtc
    14461 attgttatag tcatgataag ctttttccac tacttcttca tcaaatgttt cacacacaac
    14521 tactcctgtt tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    14581 tcgataaaga tttcctccac caaaatgaag ccatactgga tgttgctggc cttcttgctt
    14641 aacttctttg acatcaaaac acggcagtag aatctgcatt tcattgaatt cttcgataga
    14701 attttgccaa tttttaatcg aaaccataat taacctcctc aaactgatat gttagttttg
    14761 atacatttta tattttaaaa cccattgtaa ccgttgtcaa tataaaaagg gaataaactt
    14821 gcttttttct accatatcag aatgattgta ttttttattc agaaaaataa aatactcctt
    14881 ctttttggac tgattttatc gaaacttatt ttttcttttt ttcatgtatt cttttatttt
    14941 tataactagt gtattacttc aaaatttgat attatatggc atgaacagaa ttaaaatatc
    15001 agttaaaata aaagttaagg agcattgtgt atgacaatta aaactagtct ccaaaatcaa
    15061 gcttaccaat ctattcgccg acaaattatc tatgctgact tagaacctgg aaaaaaagta
    15121 tcagaaaaaa aattagaaga aacactttct attggtcgta caccaatacg tgaagcactg
    15181 attcaactcc gacaacaagg tcttgttgat acaattcctc aaagcggaaa ttatatctca
    15241 ttaattgatt tagattcggc caaaaatgct cgatttgtca gagaacattt ggaacgacaa
    15301 attatgttag agtgttgtgc caaaatgaat aaccaaaaaa aacggatttt agaaactata
    15361 atttcagaac aagaaaaagc tgttagtcaa aaaaatgctc gtagtttttt tcataatgat
    15421 aatttattcc ataaagcttg ttttgaaatt gcagagcgag aagaagtatg gcaatggtta
    15481 gaaaataaca atacacactt agaacgattt cgatggctac gagtaaaaac ggaaggatta
    15541 aagtgggaaa aaattatgga acaacattat caactatttg aggcattaac aaaacaagat
    15601 ccagaagaag ctaactttct aacatcgttg catcttcatt taatgcttaa tgagcaaaat
    15661 attgttttag ctactcatcc aaaatatttc aaaaatatat aacttttaac tctttttaaa
    15721 ttaaatggtt gttctgatca gaaaaatatc gcttgttttc ttattatgta atatatttca
    15781 taaactattc tgcataacaa ctattgtttg tactttttac attggataac taattatgac
    15841 tgctgataac ttttgatatt aaactagtat ctccaataca aataaaaaat agatatcttg
    15901 ttcatcaaaa ttagttcgtg gatacttacc ttcatttaca tcattcagcg gtttcccatt
    15961 ttcttttatt ctattatttc attttctaat agacttggct tcatttcgca atactttttt
    16021 tcaataggca tcaaattggt gttgtttaga tatttagttg tagttttgtg acatgataag
    16081 tatgctcctt tttctctttt ccactaatac aacgatcaaa gggtgctttt ttcaaaaaac
    16141 tagaaaaatc aaaataagat tcgtattttg attctatata tcctgtttca actacatctt
    16201 atatttcaca atagaataaa tagccttttg catagctgct taacttataa gaaattgcaa
    16261 tttacttctt caaagtgaaa acgaatatga gtaaaataaa attattcttc ttacagataa
    16321 ttaaaaatag cgcgtaacaa aaatcggcat tcaatcacat aattaggatt gatgccgatt
    16381 acttatattc tacgtatagt tttagtattc ttatttttta tcaccagtaa ccaattgaga
    16441 acagccctaa agcagaatag tcttttatct caaaacgctt aatttcaggc ttaggaatat
    16501 gaataatctt ttgagtatat tctttctctt ttaatcttac tgaagaaata ccactgcc
//
LOCUS       DAZKMK010000058        16335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000058 DAZKMK010000000
VERSION     DAZKMK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.058"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..614)
                     /locus_tag="ACG8V1_001830"
     CDS             complement(117..614)
                     /locus_tag="ACG8V1_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0329922.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(744..1454)
                     /gene="budA"
                     /locus_tag="ACG8V1_001831"
     CDS             complement(744..1454)
                     /gene="budA"
                     /locus_tag="ACG8V1_001831"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0329923.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1467..3131)
                     /gene="alsS"
                     /locus_tag="ACG8V1_001832"
     CDS             complement(1467..3131)
                     /gene="alsS"
                     /locus_tag="ACG8V1_001832"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0329924.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3336..3998)
                     /locus_tag="ACG8V1_001833"
     CDS             complement(3336..3998)
                     /locus_tag="ACG8V1_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0329925.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4008..4823)
                     /locus_tag="ACG8V1_001834"
     CDS             complement(4008..4823)
                     /locus_tag="ACG8V1_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0329926.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5085..5528)
                     /locus_tag="ACG8V1_001835"
     CDS             complement(5085..5528)
                     /locus_tag="ACG8V1_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0329927.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5662..6000)
                     /locus_tag="ACG8V1_001836"
     CDS             complement(5662..6000)
                     /locus_tag="ACG8V1_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0329928.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5988..6365)
                     /locus_tag="ACG8V1_001837"
     CDS             complement(5988..6365)
                     /locus_tag="ACG8V1_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0329929.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6589..7782
                     /locus_tag="ACG8V1_001838"
     CDS             6589..7782
                     /locus_tag="ACG8V1_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0329930.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7948..8370)
                     /locus_tag="ACG8V1_001839"
     CDS             complement(7948..8370)
                     /locus_tag="ACG8V1_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329931.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8765..8959
                     /locus_tag="ACG8V1_001840"
     CDS             8765..8959
                     /locus_tag="ACG8V1_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329932.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8956..9450
                     /locus_tag="ACG8V1_001841"
     CDS             8956..9450
                     /locus_tag="ACG8V1_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329933.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9612..10784)
                     /locus_tag="ACG8V1_001842"
     CDS             complement(9612..10784)
                     /locus_tag="ACG8V1_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0329934.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10990..11805)
                     /locus_tag="ACG8V1_001843"
     CDS             complement(10990..11805)
                     /locus_tag="ACG8V1_001843"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0329935.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11955..12401)
                     /locus_tag="ACG8V1_001844"
     CDS             complement(11955..12401)
                     /locus_tag="ACG8V1_001844"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0329936.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12398..12913)
                     /locus_tag="ACG8V1_001845"
     CDS             complement(12398..12913)
                     /locus_tag="ACG8V1_001845"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0329937.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(12986..13900)
                     /locus_tag="ACG8V1_001846"
                     /pseudo
     CDS             complement(12986..13900)
                     /locus_tag="ACG8V1_001846"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(13897..16224)
                     /locus_tag="ACG8V1_001847"
     CDS             complement(13897..16224)
                     /locus_tag="ACG8V1_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0329938.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4672 a         3261 c         2885 g         5517 t
ORIGIN      
        1 tcaataacac ttgtgatttt ataaaaaaat aaaaaaaccg aaaaacagaa atgcccactg
       61 tctttccggc tctttttatt cttgatttct cttctttttt gtttcaatag acgattttag
      121 ttattaataa atgaaaaagc atttatatta aaatttgcta ataaaagatc tacatatttt
      181 tctagaaatg cttgatcgat ttcatcatac cctttcgtta ctccgcaatc gatatctaaa
      241 acaccgatta aattaccttc ttttaccatt ggcactacga tttctgattg tgcggctgaa
      301 tcgcaggaaa tgtaatttgc atgggttttg acatcatcaa cgatcaatgt ttcttgtttt
      361 tcagctgatt ctccacaaac tccttttccc atcttgatac gtgtgcaaga aactcgccct
      421 tggaacggtc ctaaaatcaa ttcattacca tcgaataaat aatatcctgc aaagacagtc
      481 tcaggcaaag tctcattcaa caatgcagat gaatttgcta aatttgctaa ccagttatct
      541 tctgcttcta ataatccttg ttgctgtaat aataaaattt cgtatgcatc ttttttctgt
      601 ttttctgtcc acatgataaa aaccgctcct ttttgatcta tggagaagat tatagctcag
      661 tttttttgta aacgctaggt ttcctcctca aaaaaacgag atacgtcagg ttaatcacct
      721 tgaacgcatc tcgctgtctt tttctattct gcttgttgga tttctgctgc taaatcagca
      781 taatcaatcg tttcgtgaac aaaagtcgga tcatcaactg gaaaatgctg tacaagggac
      841 gagatgcggc taatctcaac ttttccttca gccacttcaa aatccattac atgtccgcca
      901 aatgtatgtg tatcatcgat aaaatgcaag tgaaatcccg ctgcagcaac tccttggaat
      961 aattcaggcg taaagaaccc gactaatgtt ccttggacat tgctttgttg gaattctggt
     1021 tgagactctg aaatttttgc tagtcttctg taaggttttt cttgcttcgg catgatacgg
     1081 atgtgcatat ttttgaataa tccagttatc ttcactgcca aaaataggtt ttccccggct
     1141 tctcttacta atatgtcttt tttcaattgt tctccatctg cagtttttga gacagaaaaa
     1201 ctagtatctg gcgtaaaatc tgtaattgct gcataggggg taagttcact tccatctaat
     1261 tgggcaactg agccatctga tcttccttga taagctacgc catctaaaaa gatgacttca
     1321 ccatcaagcc catgcaatgt tcccaatccc agagttcctt tttctaatat atgagcaatc
     1381 gtttctgttc catccatcaa gccagccatc aaagctccta atgtcccgtg ctggtataag
     1441 attttttcag tcatgtcgac acttccttaa tagaattgat ctggtaagat tgtttctccc
     1501 aatttctcat tatcacggta atcaatcgga atatcaataa ttacgggtcc ttctgtcgca
     1561 aatccttctt ctaaagcttt ttctaattcc tctacacttg tagctcggat tcctttagcg
     1621 ccaaaagctt ctgcatattt cacaaaatca actggaccaa agtctacacc tgacgcccgc
     1681 tgatatttca ttttttcttg gaactctacc atattgtagt gtccatcatt ccaaatcaaa
     1741 tgaacgatat tcaatttttt acgtaccgcc gtttctaaat cctgggcaga gaataaaaat
     1801 cccccatcgc cggaaactga tacgatttgt gtatttggac gcaccaatgc agcagaaatc
     1861 gcccatggca atgcaacacc aagcgtttgc ataccattac tgaataacaa atgacgaggt
     1921 tcataacttc taaagtggcg tgccatccaa atataatgac tacctacatc tacggtaaca
     1981 gtcatatcat ctgttacttt tgactgaagc gtgttaataa cttctaaagg atgcaagata
     2041 cctgcttctc cttttgattg aacaatgtcc cgctccgtta attctgcttg aagagatgct
     2101 aaatattctt ttgcatcttc tgacacttgt tgaggctcta atgaacctgt caataaatca
     2161 agagtggctg agatatcacc gatcaattct ctttcaggct gcataaaagg atcgatttca
     2221 gctggcgctt catcgatcac aatgatccgc gcatcttttt ccgcattcca gtttcttgct
     2281 tcatattcga ttggatcata gccaatagca ataactagat cacttcgttt caaaagcata
     2341 tctcctggtt ggtttctaaa taaacccaca cgaccaaaga aatgcgcttc taattcacgt
     2401 gagatcactc cagctgcttg gaatgtctct acaacaggca atgctgtctt tccaactaat
     2461 tgcctgatgg ctaacgtttc tttttcactt gatcctcgca tacctactaa tagtaccggt
     2521 agttttgctt ctctaatgcg ttcagctagg taacggatat catctgcaga agcagaacct
     2581 aatttaggat cgctcaatgg cttgatgaca ttcccttgga caggtgcatc cactacatct
     2641 tgcgggatac tgataaagct cgcgcctttt tttgaactct tagccatacg ataagcattg
     2701 gcaatcactt ctgaaattgt ttctggatct tggatttctg cactgtattt tgtgattggc
     2761 tggaacagtg ctgcattatc catgctttga tgggtcaact tcaatagatc gcttcgtttt
     2821 acttgtccgg caattgctaa aactgggtca ccttctgctg tcgctgttac caaacctgta
     2881 gctaagttac tagcaccagg accacttgtt gcgataacta ctcctggctc tcctgtaata
     2941 cgtccgattg cttgtgccat aaatgcagca ttttgttcgt gacgagtcac gattaattca
     3001 ggaccttgat cctctaattc attaaataca ccatcgattt ttgcacctgg tataccgaat
     3061 acataatcaa catgatgatt aatcaaactt tctactacag atttcgaacc ttgtttcatt
     3121 ttttcgtcca ttgaacatcg tcctttttat gctttatttt taattattag ttagaaataa
     3181 tcacaaataa ttgatacagt ttctaaacct gtttcattca aaagtatagc acgttttatt
     3241 tcatttgttt agccatatgt ttcaaaaaat gaaaaagaat gacagttttt acataaaaac
     3301 tgtcattctt aattgaaagt ttcaatttat aaagtctaca gatccatagt tctcatcaat
     3361 ttacgattgt tgaagtaaat caaaccaaat acgatagaag gacctagtag aatacaccat
     3421 tggggcttta cgaaaaagaa gagaaacaaa cttgtcaata gtaaaaaatc tagtaaactc
     3481 acataccatt ttttcaacgt atagtagaac gctatccgta gaacttgatt tggtttcgac
     3541 gctggatttc tcacctgaaa ataccagtag ttgatgctaa aggaaactga taaacaagca
     3601 agtaaaacaa acaacggaat caaccatttt ccataagaaa aacgtataaa aaagaaacaa
     3661 tcaaaaatca ttatcacact aactatccaa gcaaatagcc aataaaaaaa tccctttagc
     3721 caaaattggc gaaacgtctt tatgaacaaa cgtactggtg aaaaactttt ttcttttgct
     3781 aattcttctg tcatacctat catcacgatg caacctggac caataaaaag taaagaaaaa
     3841 ataaaggaaa agacattcct tccatctatc gacaaaagga acgcagctgc tatatacaag
     3901 atattgacac accaaaaaca aaggctactt gccaatgatt gatacaacca ataaaagaat
     3961 tccatataaa catttttgtc aaaacgctgc tctttcataa tcacctccta ttccttcatt
     4021 cctgatgaag cgatcccttc tacaaaatat ttttgcatcg ctaaaaatat aaaagcaaca
     4081 ggaagaacag aaacaactga ggcagccatg attaatgcgt actctgcatc aaatagccca
     4141 ataaacattt tcaaccctaa ctggattgtt ttattttccg tagaagacaa ataaatgaat
     4201 ggccccatat aatcgttcca tgtattaacg aatgtgatga tcgtcaaact ggcaatcgcc
     4261 ggttttgtaa gaggtaaaat gatcttgcga tagattcccc actctgataa gccatcgatc
     4321 cgcgcactct cacataaaga atctgggata ctactgtagt attgcttcaa caagaacaca
     4381 ccaaaagcat tgaacgcttg taatagcacc aacgaccaca acgtatatgt taaacccact
     4441 tttctcatca taatgaactg cgggatcata taagactgcc aagggacagc aatcgtgccg
     4501 atatatgcta aaaataaaat atcgcgccct ttaaaattta gtttagaaaa cccgtaagca
     4561 gcaaaactag aagtaaacaa ctgaatcaac gtgatcacaa cgcttaaaaa agctgtattc
     4621 ttaaaaaatg tcactaaagg aatacgagac caaataacca gataattctc taaatgccaa
     4681 gttttaggga tccattgaat cggaatcgtg aacacttcat tatttgcttt gaatgaagca
     4741 gaaaccatcc ataaaaaagg aatgatcgtc aataaagcta atacaatcag cagaatcata
     4801 cccaaccatt gaataaaggg catgtttttt gaacgaattt ttgctttttt tctttcattt
     4861 cgaacacctc caaagttata atcttcattt ctttgtaatg attacaatcc agaacaacgg
     4921 ttattatagc tgattcattt ccgttcttct tatgtttgta agcgcttatt taacaagtta
     4981 tgtaaatctc tttatttttt ttgatttaaa aatgaaaaaa tacaaaagca agactgtcat
     5041 acgtctcgct tttgtattga aatttcttcg attagagata cttattactg ttcaaaagct
     5101 tctttgaaac ggtgaatcaa ttcattgact acaggtgata aaatccgatt ctttttccag
     5161 actagcatgc agttcgtttc aatcattggg tcaagcggaa cgaatgtcac tcgctttgaa
     5221 agatgactta cagcaccttc tatgactaaa gcagcaccta cctgattctc aattagcgca
     5281 aatacattga aaatcaaatt gtacgttcct gtgatcagca actgctcctt ttttatgcct
     5341 aaccagtcag caaacattcg ctgtatttct ggacgctttg agatcaaaag aggtacattc
     5401 gcaaagtctt cttttgttat cacgtctttt tttgctaaaa cagaattttc taaaatgagc
     5461 aatccccatt tttcctttct tggtaacaaa atcgtttcgt atttctctgt atctactgga
     5521 tctagcaaaa tcgctaaatc taacaggctt ttttctagtc actccgtgat atcatcactt
     5581 gttccgctgt ggatatgaaa ttgaacgctg ggataatcac ggatcaactt ttctagtaat
     5641 agtgccaatg tattcgaatt atcagcttcc acacagccga tcgtaaatcg gccgctgaat
     5701 aattgttttt ttctgttctc gaattcttga gtggtttgat tcgttaaatc taaaatttct
     5761 tctgctcggc ttttttagaa aataacctgc ttctgtcaag atcattttgc ggttttcgcg
     5821 tacaaacagt tctgttccta gttcctcttc taactccttc agttgtcggc ttaaggttgg
     5881 ttgtgtaaga tgtagaactt ctgcggcttg agagattgtt ccttcttctg caattgtcca
     5941 aaaatatttt agtaagcgta actccatagc gatgctcctt gattttttta cttgatacat
     6001 tccattttat ttctagataa cacacgatca tatttcttga tttttccttg taaacgttcc
     6061 aaacactctt gcatttcagc aatttctttc gtcaaaagtt cactttcttc aatcaaaagc
     6121 ttacgccgct caaatacagt tgcttctcct tcgctagata ggcgaatata ttcaatcaaa
     6181 gactcgatcg ataatccggc atttcgcata cagatcgtaa agtcgaccca ttttaggtct
     6241 tcttctgtat aatcacggat tccgcctttt gtccgattaa ctggtggaat caacccgatt
     6301 cgttcataat agcgcaatgt atcggccgaa atgccctttt cttcactgac ttgtttgata
     6361 ttcatctgat cactctttct cttaatttct tgccacttta cttatgtatt ttatttattc
     6421 tacaacttta agtgtactcc aagtcaatac tcatttctat taaaactaag ctggattatc
     6481 tatatcggtt gtttgtgtat tatttcaaat aaaaaagtct tgttttttta ttcttttttg
     6541 gatatacttt taacaattta gggaaatttt aggaaaaggg gtataaaaat gacaacgtat
     6601 cagcgaaagg tattattatc gacatcggcg ggaattgctt tagagaatat ggatattatg
     6661 tttttagctt tttcattatc ttcaatgatt acttcttttc atattagtgg tacgcaagcg
     6721 ggtttcattt caacgatcac taatttgggg atgctcattg gaggtatttt ttttggcatc
     6781 atagctgata aatatggacg tgtgaaagta ttttctcaaa cggtccttct gttttcgatt
     6841 gcttctttat tgatgtattt tgcttcaaac atctacttgg tttatctctt tcgctttatc
     6901 gcaggtgtag gagcgggagg agaatacggg gcgtgtatgt ccttgatttc cgaaagtttt
     6961 tcaaaaaaac aaattggacg agcttcttcg gttgctggta ttggtgcgca ggtcggtgcg
     7021 gctttggctg cgattttagc tgctgtgatc attccaacgt ttggttggaa gatgttgtat
     7081 gtcgtaggtg tactgccggt gttgatggtc ttgttcattc gtcgcggctt gactgaacca
     7141 aaggaattca aatctgtaca atctgccgaa aagaaaacca aacttagcag actgtttgaa
     7201 tcaaaacatc ttgcttggca aacatgtggc cttagtatca tggtaactgt ccaaattgcc
     7261 ggctattttg ggttaatgaa ttggctgcct tcaattatgc aaaatcgatt agggctttcg
     7321 gtttctgggt cttcattgtg gatggtcagt actattttag gcatgtcttt ggggatgatg
     7381 acttttggca taatcatgga taaattgggg cctaaactag ccttttcttt gtttttaatt
     7441 tgttcatcct tatctgtcta tctacttgtg ttagcccata gccagtggag tttggtagca
     7501 gccgctgtga tagtcggcta ctttatcaat ggcatgtatg gtggatatgg cgcagtgatt
     7561 agtagtttgt atccaacaga aatccgcgcg acggcgaata atttcattat gaaccttgga
     7621 cgagctgttg gtggtttttc ttctatcgtc atcggtttct tgatggatca ttatagttta
     7681 actgccgtaa tcttgttttt gagcggtatt tacctagtca gtctgctggt cttgacaact
     7741 cttactggtg ttacagcatt aagggaaagc ttgcagcatt gatagttgag tctgtaaatg
     7801 acaagaaaga ggtgtggcaa aagcttgcat ctgcggtaat aagaccagcc aaatcaaaaa
     7861 catgagacac tgtttccgtt tggctggtcg tattacttga tgcagggcag ctttttcaac
     7921 tgctaatttc cttctatctg gcggcgacta ttctttgacg tccgtcattt caaagtactt
     7981 tactaataac ttagcataac gaaaaactgt ggatacactc gtatattgag cgtctgcata
     8041 atcttccaaa tttttctcag gatgttgata catgaattca atcaaatgat aattatatga
     8101 ttttctgtag aaatgaagat aaataatgta gattgggaag ttctcctgct tttccataga
     8161 aaagcgccga ttattctcat tcttcaaaat gaccttatca ggatattttt ggaacaattc
     8221 ttctaaatct gttagataat tcaagataga cttacttgat accttcaatt cgttttgtag
     8281 ttcttctatt gaacaccaac gttctgtttc aagcagtaaa tgaatcattt taattttctt
     8341 ttgagaaact tttgttaaaa aagtctgcat attttctcct cctgtatcta ctttactata
     8401 ctacatacaa gaaaaaaaac ggatacaaat atcatattgt cacaccgtca atagtgcata
     8461 aacctgttat tatttctctt tttttcctaa gtattctatt gatatgaatc atttaatcgt
     8521 ttattatata aatgtcaata atctaataat aaaataacgt acatatttta ttattgcata
     8581 cttaataaca gaactatagc aaaaaatccc gtgtatcaac acgggattag attaagtttc
     8641 ctcaatcact attcataaca caattattat aacaagtaag taaacaattg aaaaggacat
     8701 ttattgaaaa cggttgtttt ttacttaagg aggaaattta gcgaaaaaat tagttgcatt
     8761 gtttttggtc gcaggaagtt tgttgagtgg gtgtggtgct actacgtcta ctactcaaag
     8821 cacaagttca gatcaggctt ccagcagcag taccacgatc tccgaaagta cggcagattc
     8881 atcgaaagaa caagaacaaa ttgaaaagaa aaaagaagag aaacaaaaag cggaaaaagc
     8941 tgcactagaa aaaaagtgac agaagcggaa tctgctttgt cacaagctga agcaaatcct
     9001 acaaaagaaa actatgcaac tgcttcagca gctatccaag cgctacctga aaacaaacaa
     9061 gagttgacaa gccgtcttgc tgcaattgat tccacaatca aagcaaaaga agcagaagaa
     9121 gaacggaaaa agcaagaaga aattgcacga caacaagcag aacaagcgca acaagaagca
     9181 atcgcacagc aacaagcaga acaggcgagg caagaagcaa tcgcacagca acaagcagaa
     9241 caggcgcgac agcaatcagc agctgaacag gcctctcagc aggcacaagc cgcagctcct
     9301 gagcaaaata atgaacaaac tgtctatgtc acaccgaccg gttcaaaata ccatacacat
     9361 aaatgtggaa atggtacgta tacccctgcc actttaagcg aagctcaagc tcgtggttta
     9421 accccttgct ccaaatgtta tggaaactaa ttcaaaagtt atactataat aataaaattc
     9481 ttctgaaaca taaaaaaata gaagatggcg ttccagatag taaaccaaaa ataacagttt
     9541 acacgtccat ttcgtcagct tctctctttt ttcatttagt ctgttccctc tgcttttttg
     9601 gacatcttca ttcattcttt ctcaatcaaa tagtacacct ctttttcttt tcgcagttta
     9661 tatcttcctt ttttcaattt cattgaaaac tgtttcgaat gtttatgttt tacatggatt
     9721 ttaagcagga gctcctcatg ttgcaagcga tagttcctgc cttttaaatc agaaaatctc
     9781 actagtccgt attcatcaga agctttcaca aatggaactt tttctctagt aatatagtgt
     9841 ttgccgttga gcgtttttac agaaaagacc attccttcga ttggtcgtcc gtttgtgttc
     9901 tttacataaa aagataaagg atagtttcgc ttagcgatgg ccaaagaatc taaataagcg
     9961 atgagtagcc aaactaagcc gattgatcca agtagtatac cgataaacca aataaaaaat
    10021 ttccattttt tccattgatc aattccttgg atcgcttttt ttgcctcttt tgcttgataa
    10081 ggaatacgat gccccgtcac aagcagtcga tgtgtattga ccatataagg agtacaggta
    10141 agcaaagtca cgagatctct gcctttttgg atacccaaag aatctacttc atcaggtaag
    10201 accacttgga ttttttctac ttgatacgcc aatgtctttc cattgatctg gatgaaaaac
    10261 tggtctcctt ttttcaactt aggcaaatct gtaaataatt ttgcttctgg gagtccacga
    10321 tgcccagaaa gaacggcatg ggtgtttttc ccgccagtcg gataagaact ttcttccaat
    10381 aatgacgttc ctttttgcaa aaatatttcc gttgtctgat cgaatatagg taagctgaca
    10441 ttgatttttg ggatttcgat gactccaagc atatgttcca taaaaaaggc attactaggt
    10501 aaatctttta atgcttttgc atcaactgct tggttgaaac tgcctatccc tggtgatacg
    10561 ttttgctctg ccaactgttg gtttttcttt gtcatttttt cctgctgttt ttttatttcc
    10621 tctgaattct tattcgatgc ttgcttttga taatggatga tcatttgctg ggcaagaaaa
    10681 tcattcagtg aatcttctac aaaaggataa gaaaagatcc cgatcccaac gactaaaatg
    10741 attgccatga agatgtccag tattcttatt ctgcttttat tcatcatgtc cacctatttt
    10801 ctaatatgta tatgaagagg ttgtgaaatc agcgccttga cctgagtatt agcgaagcaa
    10861 acggcaaaat agttcctcct attttgccgt ttgttgagcg aataccaaga gaccgaccac
    10921 ttgtcacaac ctttctcccc atttgaatca ctatcgttta tttatcgtat gatttttgtt
    10981 tccaatagtt taagcttctg tttcttgatg gcgttttttg aaatagatca ctgctcctcc
    11041 aactaaagca gtacctacca agatgaatac gatgatcccc ataccaccag ttgaaggcaa
    11101 gattccttta ggttggttat cgatcgttgt ttcatcagaa gttgttggat catcatctac
    11161 tgtaaattca gtgtacgcag cttcattctt aggtaatgcg tagccttctg gtgcttttgt
    11221 ttcttgaagg cagtatgccc cttgtttcag cccttcgatt gaaaatttcc cttctgaatc
    11281 agaaatcaat ttcacttctt cccagtcaga acctgtgact tcatctgacc aagcatatgg
    11341 gtattctcct gaggcaaggt ttgctatttt tttcattaat ttaacagttg gatcttttgc
    11401 tcttagaata gaaaattctg catcggctaa tttttcttgc gtgttcacat cttgtttgat
    11461 gaagtggaaa tcaccgtagt tttggacatt ttctacagta atcgtgttat ccgtagttgt
    11521 attttcccga ttgactacac cgtttggatt cccaagattg gcttcatctg cgccatggat
    11581 gataccggtt gtttgttgca attttccgtt ttttactata aaagtagttt ctttttgtgg
    11641 attggttaat tgataaccat ctggtgcttt tgtttcaact aaagtataat tgccgtctat
    11701 taatccattg acactgatgg atccttcagt atcagttgtg aatgtcaacg ctgactcagc
    11761 aggatcatag agagcgacac cagtgttttc atcaaaaccg gtcacgaatt gattctctga
    11821 atttttgatc tggaattgcg cccctgctaa cttatctacc cctgaaaagt tcccagcttt
    11881 ctggattttg atattgcttt ctttgatgac atttttagca tataggtgaa tcgtgtctaa
    11941 aggtttgtca gggtctaggg taagcttaac acgatatttg gcgacgtttc gacaccgggc
    12001 tttggtgttt ctacaaagac gtatacagca tctttcccat cagatttacc tggaagagtc
    12061 aggttagcta atccttcacc agaagtcgtt acacttgcga agggtgtagc gtctgctgga
    12121 actgctgtgt ctttacttgg atagtacgta gcagtgatgt catatacgtt gaattcaacg
    12181 ccgttcaacg gttcgctatt cggaaatgtc aattccgttc cgttgtttat ttgattttct
    12241 ggtgctgaag tgaatttcaa tttatgcaaa ttaaccgtaa cattttctgg tgtaggtgat
    12301 acggctgcag aaacctctgc agttcctagg ccgagaacga aaggaagcag catgatagca
    12361 gttcctaaaa acgtccatat ttttgttttg atgtttttca ttttatttcc ctcctattgt
    12421 ttcctttttg ttgtttttta taaaaccaag ctgtccctgt tgaaaggact agcaataacc
    12481 ctaaaattga gaaacctcca agcatctctt cgcttgtttt tggaaagctt cctccatttt
    12541 tttcatttga tggaccattt gtctgcatct gaactggcgt ttttccatta ttcgtcggat
    12601 ttgcttttgg ttcgttgatg atttccaatg gtataggatg aagcggatca taactgcctt
    12661 tttcaacttc aaagagaatg gttgattgac tcaatatata tcctttaggc ggtttaatct
    12721 ccactagttc gtaacttcca aaagacaatc catggacaga aaatgttcca tctttactgg
    12781 aagtaaaaat cgttgctctt gaatcatctt tggctaccca atgatttatc ccgttttctt
    12841 gcgctaagta ttggtcattt tttttgacta gaaattgagc atctgctagt ttttgtttcg
    12901 tttgccgatc cactttgaca aattgtttgc ctcctgtttg gatggcaaga tgtgtttcta
    12961 ctgtttgatt gtcagtcgtc aaacgtacat cgttatcaaa tgtcgttttt tcactgtttt
    13021 ctttcaatgt catttggtaa cggatcgtta atttttttcc agcaaacgaa ctaagatctt
    13081 ttccagaatc tgataagaca aaaccatgtt ttattttttg tgtgtgaaat gtatgaatct
    13141 tgtctccacc aatcaaaaaa tcgatcgttt ctggttctag ccataactga ggatcagctg
    13201 tatctttgat cgtataggtt cctatctcgt tgatgttctc aggaattgtt acggtgatga
    13261 gataagggac tttatcccca tctgcaaaat ctgtctgctt attctctact gtttttgtta
    13321 atgtgagtgc agcaggtatt ttttgttcgc tctttggata caagtggatg gtcgtcaatt
    13381 tatgattaga tgaatctgtc acaggaagca caacgacaaa tggtgctgat ttttctttta
    13441 cttaatctga agtctttatt tccgtaaatc ggtaaacagc gtcacgacct tgctcatctt
    13501 tgtccgatgc actaaaagaa gcaatgccct cttcctcgtc tactgttttt gtcacatttt
    13561 ctgccagtat tttttcacta tcactttttt gtgctagttt tctttgtgcg tcttccacac
    13621 tacttccttc tgaccgcagc ttgtaaaatt catccgagac atcatatata gcgaaagtaa
    13681 cgccgttcaa cccttttact ccttgaaagt tatccgggat atttattccg tcgttttgac
    13741 tgatacttgg catcttatcg ctttcgaaca ttcgattatg caaagtaaat gtaataggtg
    13801 attctctagc tgacacagtc ggagaaccta taaaaaagac cagtaggata aaaaagagaa
    13861 cagcaaatat atccacacgt acattttttg tttttttcat cgctgttcac ctgctttttt
    13921 cttacgatgg ataaaataaa agaagccaga aaaaataaag atcagtccta aaaagacaaa
    13981 gggcagtaga cctatcccac ctgtcgaagg aagcaagaga gtcggattga tcgtcaactt
    14041 agcggtatct gttgcgataa tcgtataatt attgtttaca cttccttcgc ctaaactaat
    14101 cgttactatt tgtgatccaa tatctgtccg gctgttaact gttagggtat aatccaatga
    14161 ttcgcctaag acaacaccgg taacgtggag atttccaacc gaaatcgagt gactttgggc
    14221 atcataatta aaggcagcgt catcgacagt aatcactgcg ttagctttat agatggtata
    14281 agttcctgat gtgttggcta tatcagtaaa tgacaacttg ggatttgctg ctttgacttt
    14341 gtctattccg ctattcgtta gtttaattgt atagctgcca gcattaacca tattttttgc
    14401 tgattgtgca tctgctgtgt aatccatact gctaagtgcc acttgtactg gagtaagcga
    14461 actagtgaca ccctctgcta gcttgacatg gattgacggg acacctgctt ttccatcata
    14521 tgtcttactg ccactgagct ctaccgcacc gctgatttgc ttaatcgtat agacatcaga
    14581 tgtctgcacg atagtaaata aatagtttgt tctggcagct agtttagcta gtccgctttt
    14641 tgtcaattgc caagacgtat aggttccaat ttcagtaggc gcagttactt caactccctt
    14701 ttcattcatc gctttgaaat cagttggatt ccatgtgatt tgatcaatac tgagatcatt
    14761 gtttaaaagg gatgttgatt ctgttttatc attaaaggca tatttaacga tcggtacaac
    14821 agaaagacca tcatagtact tactgccatt tgcattgatt gatactgttg catttgtctt
    14881 ataatatagg atgacttcag aaatctgttt tccatttgcg tcctttacga ttacatttcc
    14941 atttgtatct aaactagcta aaacactcat tgctgattgg ttctttgttt ctatcgctga
    15001 aacagctgta aaggtatatc cgttgatagt aggcgtatta gtcgtcaggt cttgtgcagc
    15061 acttccgtaa gtaagattca ttattttaga aggagcaatc atctgtccaa aatagtcttg
    15121 atacttaata gtgattggtg tttgtttgcc tgtataggtc accgtatcga cgttactttc
    15181 tagggctgtg aaagaaccgt ttgtttgtgc aaaagaatag cccgttactg ctgttggagt
    15241 gataaccact ttgtcaccca catggattcc agataatgta gtcccgtacg ttccgatttt
    15301 tgctgctcca ccattgactg tataagaaaa agttcctgtc cgattcgcat tgacctgaac
    15361 cgttgctgct tgataggttg gaaaatttga aacatttccc ttaccatcaa cgatctgccc
    15421 atttttgatc gagtaccccg tgaatggaat atcatttgct aactgtatac cgattgaaaa
    15481 agtactcata cctcctccgg caggaagccc tccccatgtt gagggtgtaa aagaataact
    15541 tttatccgca ttgggtgtaa ctgaatcgat cgtatttcca gcggtagcca cccccatata
    15601 gatacttttt gggaatgtga tagtagtacc atttactgtg gagccaataa acagattatg
    15661 gatatctgga cggttatcaa taaaagcgat tgctttggtt acgccttgta acgcagtttt
    15721 atccgcactc atcgatccgt taaccgcaac aatgactcca tctttggcat atatcactgt
    15781 atatccatct ggtatggtat gtccgttgaa attcgcagtg gccgcaacag tactaaatgt
    15841 ctggcttcta cctgtttctg aataagcttt ctcccctgct gcttttgctg ctgtgatttc
    15901 tttttctgtc ggatcaatca gttgagtcga ggatgtggtt gaggttgtct cgttgttgct
    15961 agactgattg gtgctttcta tggactgtat ttcagaatag ggtggactat ttgtctcctc
    16021 tgtcgtttgg ttagcaaaat ttgacgtttc tgtataaacg tttacataaa tacttgataa
    16081 gctccctaag aaagtaaaaa tcaagatgat cgaaatcata atgggatgta agtattttct
    16141 tttagataca gctttgagtc tagaaacaaa atgtttttga catatacgtt ggattttatt
    16201 ttgagttttc ttaatcattt tcatccctcc tctcaataaa aaatcatcaa ataatccttt
    16261 acacgttatt ctaaatttaa tttttattat tttattgatg tatagaaagt ttggggcttt
    16321 ttttctcttt ttatt
//
LOCUS       DAZKMK010000059        16284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000059 DAZKMK010000000
VERSION     DAZKMK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.059"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..1547
                     /locus_tag="ACG8V1_001848"
     CDS             120..1547
                     /locus_tag="ACG8V1_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0329939.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1639..2004)
                     /locus_tag="ACG8V1_001849"
     CDS             complement(1639..2004)
                     /locus_tag="ACG8V1_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0329940.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2001..2366)
                     /locus_tag="ACG8V1_001850"
     CDS             complement(2001..2366)
                     /locus_tag="ACG8V1_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0329941.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2558..2806)
                     /locus_tag="ACG8V1_001851"
     CDS             complement(2558..2806)
                     /locus_tag="ACG8V1_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329942.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2805..2933
                     /locus_tag="ACG8V1_001852"
     CDS             2805..2933
                     /locus_tag="ACG8V1_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0329943.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2990..4684
                     /locus_tag="ACG8V1_001853"
     CDS             2990..4684
                     /locus_tag="ACG8V1_001853"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0329944.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4784..4957
                     /locus_tag="ACG8V1_001854"
     CDS             4784..4957
                     /locus_tag="ACG8V1_001854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329945.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5335..6468
                     /locus_tag="ACG8V1_001855"
     CDS             5335..6468
                     /locus_tag="ACG8V1_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0329946.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6499..6819
                     /locus_tag="ACG8V1_001856"
     CDS             6499..6819
                     /locus_tag="ACG8V1_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0329947.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6848..7312)
                     /locus_tag="ACG8V1_001857"
     CDS             complement(6848..7312)
                     /locus_tag="ACG8V1_001857"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0329948.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7444..8697)
                     /locus_tag="ACG8V1_001858"
     CDS             complement(7444..8697)
                     /locus_tag="ACG8V1_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0329949.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8726..9043)
                     /locus_tag="ACG8V1_001859"
     CDS             complement(8726..9043)
                     /locus_tag="ACG8V1_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0329950.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9069..9389)
                     /locus_tag="ACG8V1_001860"
     CDS             complement(9069..9389)
                     /locus_tag="ACG8V1_001860"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0329951.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9853..11268)
                     /locus_tag="ACG8V1_001861"
     CDS             complement(9853..11268)
                     /locus_tag="ACG8V1_001861"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0329952.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11285..12601)
                     /locus_tag="ACG8V1_001862"
     CDS             complement(11285..12601)
                     /locus_tag="ACG8V1_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0329953.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12751..13449)
                     /locus_tag="ACG8V1_001863"
     CDS             complement(12751..13449)
                     /locus_tag="ACG8V1_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0329954.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13738..14727)
                     /locus_tag="ACG8V1_001864"
     CDS             complement(13738..14727)
                     /locus_tag="ACG8V1_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0329955.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14807..15640)
                     /gene="msrA"
                     /locus_tag="ACG8V1_001865"
     CDS             complement(14807..15640)
                     /gene="msrA"
                     /locus_tag="ACG8V1_001865"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0329956.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15815..>16284)
                     /locus_tag="ACG8V1_001866"
     CDS             complement(15815..>16284)
                     /locus_tag="ACG8V1_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329957.1"
                     /translation="NFNQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENK
                     EHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVK
                     EQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5069 a         3281 c         2765 g         5169 t
ORIGIN      
        1 aaaaaccttt tttaacggca tttatatgct ttttcagaaa ttaactgatg atttcaaaaa
       61 gaaaacatgt cataatattt cccatattgt gcaaaaaaat acaaggcgga ggctctacta
      121 tgattgaaga atatattgag aaaaatattt taagacagct gtttttatgt gggcaattct
      181 atgtaaataa agaagtcaat ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca
      241 ctttactcaa tgatatcaat aacataaaaa aagaatttga agaacagatc gcttatacac
      301 atcgggaaaa ggaccgttac actttatatt tttcagaaca tatacctcga tgcaagatca
      361 tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg tctgctttat ttggaagaag
      421 atgaacctga ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc aaagcttatt
      481 cattaaaaaa acaagtatta gcttatttta atgactgcgg gatcgaaatc gaccgctact
      541 cccctcgatt cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag
      601 gcggttttaa ttcttgggag ctacctgaat cgttttttga acgtgccgat cgtttcattg
      661 aatccgtaac ggaaaatagt ggcagatttt atgataaaga aaataaagaa atcttatcga
      721 ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat
      781 ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt cgaatcagca tgggaaaaca
      841 ctgatttcca aacctattat aaaaaagaag aattcgcctt tattttgaca ctattcaatc
      901 tttgtaatta tggtttccat tcttatcagt tgattgcgga agactttcaa cagctacacc
      961 aagtgtttat tgacaatact cccgaaataa aagaactagt tgctaccttt gaatctcact
     1021 tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt
     1081 cggcttggga taactatcaa ttattcatgc cagaaaaatt ttatctttta aatgaggaac
     1141 agacaaattt atataaagaa gtccagacaa ttttttccag ctggtccagc cagttacctt
     1201 atgatctacg gttgaatcct aattgcatgc gggcttttgt gatcgaattg agcggaattc
     1261 tccgtctgac aaaagaacat ctgactatct atatcgtcac aaattcggat gttcattatc
     1321 tgatttatcg ggaagccttg gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa
     1381 tttattcaag tatatctgat ataaagaagt atgcgcagca gtcctcaaac cgggtattat
     1441 gtgaacgtac attatataca ccagatgcgg tccaatatga aaatattatt cctatttcga
     1501 tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa
     1561 aaatcatgtc acagcctctt atttgttgaa gatgcccatt tctgtaacaa tctcttgata
     1621 cacgtcaggc tcacaacctc acttattttg gacatctacg ggtccgatct ctttatttaa
     1681 aaaataagaa atgatggttc ggataactac caacgccgct agttggaaaa tatcctgcaa
     1741 ggtaggtttg ataatcgatt cgatgatccc agctgaaaca agaatttcca aacttagaag
     1801 tatatagcct cccaacatct ttttaatagc attgttctcg ctgactcggt cgctttgcac
     1861 ttttgtatgg acaaataaat atttcaaaaa atccttgatt gatagacaaa caccccaaac
     1921 caataaacag atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat atggaagaag
     1981 aaactgcatc aactctgtcg tcatcctacc tctcctcttc tttgtttttc tttgtcttct
     2041 atttcctgat tcaagaaata agaaatgact gttcgaatga taataataag tgcaagcttc
     2101 aaaatatctg tccaagtcgg cttgataact gactcgatga tatcagcaac gactagtact
     2161 tccaagctta acaaaatata acttgctagc agttttcttg tatctgcatt acgagtatta
     2221 cgttctgcat agtttttctt gacatgagac caatcaaaaa gtttgaatag aacaagaacg
     2281 atcccgacaa ttaagatacc gatagaacaa atatttaaaa tagcaatgat ccattctaga
     2341 aattcaatta aatttgagcg aaacatatac gctacctact ttcttttaat ttgttcctat
     2401 tcttttattg taaaagaaag ttaccttgtt cgtctattta aaagttgttc taatgataac
     2461 ggttcgctta aatttttctt tttcactctc ccatttttct ccctccatcc ataaatcgtg
     2521 gaagtatcca aaacaacgta gcaagttaaa ataaggctta gtccgtcatc agcttttcaa
     2581 agaagcactg tctcggtgct ttttttgtac ccaaatttct aaaaatagaa ccatacacct
     2641 cctcaaatct tttcttctta cctaccctat ttttatttac aggaaaatca gacacttctt
     2701 cttttttgtc aaaatttcct ttttcttctc gtcctatcat ttggcattct aataaaaacg
     2761 aactatcctt ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa
     2821 tcaaagtcgg catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaga
     2881 caggactatt cgaagatgac agtcatttat atgatgaatt cgaatcaatc tagcatccta
     2941 tttagttgaa tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag
     3001 taaagcaata atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca
     3061 agaaggcaaa tggaaagggg atcagatcac cttttattca ttggatgatc acggttcaaa
     3121 tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc atccattaga
     3181 aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga
     3241 tttgatggat ctgttaagtc gaataccttc tgttactgag ccagggatga ctgctgaaga
     3301 agatacgaga gattttgatg caaaacaccg aacctttgac aaagcacgtt tgatggaagg
     3361 tggcaaagga atcattgatg gcggaaaact gggattcaat aataaagacc gtattcttct
     3421 gactaaactg attgctatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga
     3481 agaatatttc aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac
     3541 aacatttgct ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat
     3601 gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac gttacaatca
     3661 atacgaaagt attatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat
     3721 tttgaaccgc cgtgtcactg actgggaatt caaagaaaca ccgatgcaag atgaaatcac
     3781 tgtgactggt ttgaaaatta ccaatacact gactcaagaa gaagaatacg ttgtggtcga
     3841 tgaagataca gcagttattt tcactaatgg gtccattaca gattcagcta ctttaggaga
     3901 ttacgaaaca ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa
     3961 agccagtgaa cgtttctaca acttgggaaa tcctgacaaa ttctttgctg atcgagatgc
     4021 aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat ttgttgttga atgaaatcac
     4081 ccgtatcacg actcaagaac caggaaatgc cttgaattca tttatttcaa ccacaccgat
     4141 cacgccgtta ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc aaccgcattt
     4201 tacgtctcag aaaccaaatg aaacagttat ttggggttat ttcctttatc caagacgacg
     4261 tggcgaattt gtggataaac agtatattaa aatgaatgga aaagaaatgc tagaagaact
     4321 gattggacaa ctttcaaaag tagatcctgg accagtgaat attcgtgaaa aagaaacaga
     4381 aattttcgac agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcgttgtt
     4441 caataatcgt gccaaaagcg atcgaccaaa agtcattcct aaacattcga ccaatctggc
     4501 ctttacagga gaatttgtcg agcagcctta tcagatgatc tttactgaac aaagtgctgt
     4561 acgttctgga gaaatcgctg ctttccattt tgcgggtatt cctatgtccc gtcttgtcaa
     4621 aacaccgcgt tacgacaaag atattcctac tctagctcgt gccgcaaaaa aaatgtttga
     4681 ataatcaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaggctaaa
     4741 acagcttctt atgtttttga tttggttgga cttattaccg cagatgcaag cttttgaaca
     4801 ccgtttacca agaggttatg aaaaagctgc cctgcatcaa gtaataagag cagccaagct
     4861 aaaacagctt cttatgtttt tgacttggtt agacttatta ccgcagatgc aagcttttga
     4921 acaccgttta ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt
     4981 gtgaaaatgg ttcctcacgt ttttgcataa atttgactta ataccgagaa actgatccct
     5041 tctgtcataa cctcttttta tttatgtctg tttctttttg aaaacaacta ttttttcaac
     5101 gtttactctc ttgccaatcc aacatcatct aacggataca actatttcaa tgattaccat
     5161 tacactaaaa aatcggaaac acacctctcc gaacacaaat acataagact atagaatgtt
     5221 tcatttaaga aaaaaagcgt ttcattatca aaaaggtaag atctccttta aaatgaaatt
     5281 atctgtagta ctaataatgc agacaatttt atgaatctga aaggagcatc tccaatgaaa
     5341 gcagtcactt ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga tccaaaaata
     5401 ttagaaccaa cagatgcaat catccgtatc acagcaacag ccatttgcgg ctccgatctg
     5461 catctttacc atcatggtga agcagttttg gaaaaagggt atgttgtagg acacgaacca
     5521 atgggaatcg ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg
     5581 gttatccctt ttaatattag ttgtggtcat tgtcatttct gcctgaatca catggaaagc
     5641 caatgtgatg aatcaaatcc agatcagtta tatggcgggc ttttcggatt tggcaagttg
     5701 aacggtaatc attggggtgg tcaagcggaa tatctaagag ttccttatgc agactctact
     5761 tcatttgttg taccagataa tgatcttcct gatgaaaaag ttttatttct atcagatatt
     5821 ttgcccacag cttattggag cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg
     5881 attctaggtt ctggtccagt cggtctattc gctcaaaaat tcgctgttat ggctggagca
     5941 gagcgcgtaa tcgcagtaga tccagtacag caccgtctag ataaagcggc taattacaat
     6001 ggggttgaaa cttatttctt agaagacacg gcacaagctg gaacagaatt gtatgaactg
     6061 gctaaaggtg gcgcagatgt cattatcgat tgtgtaggaa tggacggatt agagcctgtc
     6121 aaagaaaaag cgaaaaattt agtaagctta caaagcggca caatctctcc attacagatg
     6181 gcatcgcaag ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta tatgactcca
     6241 gcttcttctt acccgctgca agaatttttt atgcgggata tcgctgtcaa gcatggacag
     6301 gcaccaatta tccatctaat gccaaaaatt tacgcaatga ttgcagaagg actattcgat
     6361 cctacacaaa tcattactca ttcgctgcca ctatcagaag cagcacaagc atatgagatc
     6421 tttgacaaaa aagaagataa aaatataaaa gttgttttga aaccttgaat tcagtaaaaa
     6481 atacaggtga ttttctttat gcccaaaatc aaacgaaata cccatgagat cgaaatagga
     6541 aaagaagaag atatcgaaat acgaggacgt cgcttccgct tctatttcca aaatcgttac
     6601 acattgatct cactggctgt cgatttatta accgggattt tctatatttt tggaagcctt
     6661 gcttccttaa ctaaaatccc cgatcgatat ggcacctatt tttatttggc tggtgctatt
     6721 ttcttgacga ttcgtcctat tctcagaatc gtaaggaaca tttttattta cgacgaaaaa
     6781 aaagcaatga agagaacaag caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc
     6841 tcttttttca ccctatcgta tcactttgat acagacgagc ggtcgtccca ctttcttcct
     6901 tgaccattgt caaaaatgtc cactcgtatc gttcataaaa cgtggtatga tctgtaatta
     6961 agtaaagttt ttcgtaccca agttgatgca tttcttttct gatttccgct aatatccgct
     7021 tggctatccc ctgcttacgc ttttccggtt caacaaacag ggcacaaaga tttggtgtca
     7081 gatcagggcg gttatggaag tcgttttcaa tgacgcccgc tcctccgacg atggtttctt
     7141 tttcatctaa gaccacatac cattgaggaa tactttcctt attagcgatt ccagtacgaa
     7201 tactttcctc atacacaaca gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt
     7261 gttttactaa ttctgggtgt tctcttaatt tgatccagtt atatgtaacc aatgtttaaa
     7321 cctccgacta aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa attagcaatg
     7381 acaacaatag tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc gttttttgat
     7441 taatcaagca tctaaagctt cttttcttct ttcttggatc gtagctacct tgacgaacgg
     7501 tagatagatc aaaatagaaa gaaccaaatt gacagcggct agtacaccac ctgcaatact
     7561 ttttgttgcc aatacaccgc caataattgg tggagttacc catggaggca taacggtagc
     7621 tgctggcact aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga ccattggtgt
     7681 caaaataaat ggaatgaaca tgatcgggtt caacacgata ggtaacccaa acatcgttgg
     7741 ttcattaata ttgaagacac ctggcgcaat cgacaagttt gttacaacca tatatggttt
     7801 gtttttacgt cctactaaat aaatagctaa cagtaaacct aaagttgcac ctgttcctcc
     7861 taagttgaca aagctgtcaa agaaaggttt gttcacgata aacggtgcgc tatgtccagc
     7921 tgcgattgca ttaacgtttg cttcaatggc tggcgcattg attgtttgca tcaaaggatc
     7981 gaccatattc gctccgtgta aaccaaagaa ccataagaat ggtgtgataa atgcaagcag
     8041 taaggcagat ggataagagt ttgcaagtcc cataaatggt tcttggacca ctgtatagaa
     8101 agaacctacg atatcatcaa caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc
     8161 agagatagtg atcattgctg gtagtaatga agcaaatgct tttgcaactg ccggtggcac
     8221 accatcaggc attttgatga tcaatttttc atttccgctt aaacgtacaa agatttctgt
     8281 tgaaataatc ccaacaataa tagcaataaa caaaccgttt gagctcatac ctaatgctcc
     8341 gcctaaagcg aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt cttttcggta
     8401 gccgttagct aaatgataag caagcaagaa agcaatcaag acagaaagaa tggcaaatgt
     8461 accgttccaa gcagcagccc cgaatccttt ccatgattca ccaccaaaaa ttgagttcat
     8521 caattcttgg aacccgggaa tcggtaagtt attgatcatt actgctaatg ctcctaagat
     8581 cattaacggc atactcacca taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc
     8641 aatttttgat gctacaggta taaaatgttt ctccataaat gcaacaaatt tatccataaa
     8701 tcttccctct tttctttact gtagattacg cgttcaatcg acgatacaga tcaatgatct
     8761 cttttgctaa atctgtaaaa gctatagatg tcatcagatg atcttgactg tggaccgtca
     8821 aaagcgtgac ttcaacgtga tttccttgtg cttcttgtgt cagcatctct gtttgagagt
     8881 gatgtgcttg gactaaagat tcctctgcat ctttgatttt ttcatctgct aaagtgaaat
     8941 cacctttttt cgcagcagca atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca
     9001 tgatcagacc catgatggct tctagatttt tttgatcttc catttattgt atcctccgat
     9061 aatttttatt atttgtttat tagtttttct gcttggtcga gtactttttc accattcatc
     9121 atgccatagt cagccatatt gattacatct aaaggaatgc ctttaggtgc tagcttttca
     9181 gcaaattgat tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta
     9241 gattctagtt gattatccgc atctgaagca gatacagcaa agatgtctgc ttccattcct
     9301 tttgcttctg ctgccttttg catttttgtg actaggagac ttgtactcat tccagctgaa
     9361 catacaagca tgattgtttt cttttccatt ttaaacacgt tcctttctat acttacactt
     9421 gtatataagc aagtagcgtg ccaactttaa aagcgcttta aaaagttgat atcatcacat
     9481 ttaaggtcta taccaagttg catacacact aaaaaagaaa gcgcacacta aacacacaaa
     9541 ttaattttta ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg
     9601 tcacaacttt tttcaaataa acggtattca aaagcttgca tctgcggtaa taagtccaac
     9661 caagttaaaa acataagatg ttgtttttac tttgctattc ttattacttg atgcaggaca
     9721 gctttttcac aacctctcaa taaacggtgt tcaatcagct gaccctgggt attagctcaa
     9781 aacgctggat tcacaacctc ttttatttca aataaccagt aaatacaatg aacatctaca
     9841 aaaaacttct atttatttat agatttcgtc aggcaaatcc aattctctct tatcaatcaa
     9901 agtctgatac cacgaagcag attttttcaa tgttctgctt cgttcatcgt tcaattcgat
     9961 tcgaactaat ccatatcgat tcttaaatgc attcattggt gacacacagt cagtgaatgc
    10021 ccataacata tagccttcgc aatttgcgcc ttcttctgtt gctttgatca agtaatgcaa
    10081 atgctgtccg ataaattcga tacggtaatc atcttgcacg attcctttct catccatata
    10141 ctgttcttca tattctcttc ccataccgtt ttctgttatc agccaaggga tattatgata
    10201 attttctttt agatacatgg ctatatcata catgattttc ggatagattt cccatcccct
    10261 ggaattattc attttcttcc caggtaaatc aaagacttca taatatcgtt caggatggaa
    10321 aggtgtctct tcattccatt gataacgagg ggcgcgaacg cgtttaggat aatattgatt
    10381 gattccaagg aaatctactg tatgatgctg aatcgtttct aaatcctctg ttgcaggatc
    10441 gaaataaatc gattgttttt gacacaactc caggaactct tctgtgtagt atccttttac
    10501 catcggatca aaaaatactc gattataaaa caaatcatac atataagcag ctttttggtc
    10561 ttctggagaa ttggatcgag catagaccat ttcaggattt aaaatacagc caatccttcc
    10621 accataattt ccgctgcggt aaatctctac tgctttagcc gttgctaaaa ctttatgata
    10681 attccacatc atccactttt ttgtgttttg ttcatgcggc cagcgaacag catccaaata
    10741 gcaacgcgtt tggggaacga ttggttcgtt gaatgtaaac cagtttttta ccctatcacc
    10801 gtaacgatca aaggcaattt gagcatactt agcataaaaa tcaactactt ttcttgaaga
    10861 ccagccatca tatttttcca aaagaaaagc tgggacttca taatgttcca aacaaatcat
    10921 cggttctacg tctgcttcga tcaaagagtc gatcatttca cttatatgct gagcatattc
    10981 ttcatctact atacagttct cataatcagt aaaaaaacga gcccaattga tagaagtccg
    11041 ataatgtgtc agatggatgg atttcatcag atccacatcc tgctggtatt gttccataaa
    11101 attagttgct acggctgggc catacccatt atgccaaaca aaatgttcat ttttatacca
    11161 gctatctata aacgaatcct gtccttcttt ttttcctttc caaccttccg tttgccaagc
    11221 agaagatgca gcaccaagaa taaaattttt tggaatagta attttcatga tttcctcctg
    11281 ttttttagat cccatcattt tccacacgat ttgctacttt tacaaatggc agatagatca
    11341 aaatagaaac aataatacaa acaagttgcg taataactgc accgatattt cctgctgttg
    11401 ccagataagc attgacgatc ggcggcattg gccaagggac cattacaact gctttcgccg
    11461 caaatcctaa agcggttgca aaataaccaa tcgttccagt gataagaggc gttaagataa
    11521 acggaatagc taaaattgga ttcagcatga tcggcatacc aaaaataact ggttcattga
    11581 tgttgaagat cccaggagca agggataact tggtgatttg tttcatatct tcccgttttc
    11641 caactaacaa tactgcaatc agtaaaccga tcgtgactcc agacccgccc atactcatat
    11701 acatatccca aaatggcata gtaataatgt tcggtacttc ttttccagct tcaaaagctt
    11761 ccgtatttac agcaattgcc gctaaaagca acggttcccg tatcggcttc accatttgat
    11821 ttccatggat accaatcacc caaaagactt gcgcaataaa catcaatagc aaaatacccg
    11881 gtaacccttg tacgacacct tctaatgggc gttgtacgac attgtaaatg atatcataaa
    11941 ggtacattcc tgtaagttct ttgatgataa atcccgcagc cgcgataccc gatacagtca
    12001 gaatcgtcgg gaataaagca gaaaaactag cagaaacatt cgttggcacg gtatcaggca
    12061 ttctcagctg tagtttcttc tgtttcctca accagcaata aagttcaata gaaaggatgg
    12121 agaccaacat tcccaaaaat aaccctttcg tatctgtata ttgtttagcc aatacgtttt
    12181 ctactataac agtctgattt tcattcacta attcataaac agttggatta acagataaat
    12241 aagacatcac tgcgatgata ccagggaata caccatccac tttattcagc ttggctaatt
    12301 cgatccctat cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca tagctgatgg
    12361 cagatgcgat aggctttaat tcttctaaaa aacgcaagaa acttatctgt gctaagccat
    12421 tttccgagtc aaagaccatt gctgaaaaca atgtgccaaa agcacccgta ataattaccg
    12481 gaatcaatgc agcaaatgcg ttttttatca ccatgatata ccttaaatta ttgattctcg
    12541 ttgccacgtt ccctaaaaca ttggctaatt tatccataaa actcattcca gatcccctca
    12601 ttcctgcttc ttctccttga cgtcatctct ttttgtctat tagtcagcgc ttacatttcg
    12661 atgaacagaa actatttact cctctttttt accaaatata aaacaaaaat tcatctgatg
    12721 tatttatcat aaaatacatc agatgaactg tcaattagtt tctttttctc ctattatttt
    12781 ctgtaattct ctccgattta ttgccatatg gacagtcatt gcatcaaacg cagcaagtgc
    12841 attccgcctt tcgatggcct gaacgatctc tgtatgaata tcgatcgtct cgattttcag
    12901 atgctggata tcataatttt tattaaacaa agcaattgat tcattaatga ttgggataat
    12961 atgcagcaag gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat cgatgtctaa
    13021 ttgaacatgt tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg ttaatttttt
    13081 catttggacg atttgattct tcgttgcata ttccgctgcc aaacctgcca tcttaggctc
    13141 caataaatat ctcagttcaa ataagtctcg tatcatccga tcctgatctt caatcaaaga
    13201 aaaaccgagt gggtcattgg caatccctcg cttatcactg acaaatgtcc ccgctccttg
    13261 ccgaacttca aggatattcc tggaagctag agcccgcaca gcttctcgca ccgtgcttcg
    13321 ccccacctct aaataatcgg acaattcata ttcattcggc agtttatctc ctacttgcaa
    13381 ctgcatttcc tttaaatatc taaggatctt gtcagtagtt acttctacta gggttttatt
    13441 ttcttccaaa ccacatacct ccttttcatc tgatgtattt tttcgtaatt attataacac
    13501 gtttgaatca tctgatgatt ttttgtatac aaaattgatt aatccgttca tagacataca
    13561 aacgaagaag atatttacca taaaaaagac taggataaaa ttcactccta gccttcgatc
    13621 atttttattt tgaacgacaa aaaatatgat gttcaataac agacgatctt cattcgattc
    13681 ttgatgtcat cttttgtttt ttttaatata tggataaaaa gtttgattac atcgtttcta
    13741 ttctagaatt tggtcgattc tcaattcttc ttctggtgag aaatctaaat tatccatcat
    13801 tcgaacactt tcttgtaact gacttaaccg acttgctccg accagtacac ttgtaacaga
    13861 tttttgtcgc aaattccatg caagggccat ttgcgctaat gattgtcctc gtgatacggc
    13921 gattgtttgc agtgctttga ttttttccag agttgatcca acttgttctt ctgaaaggaa
    13981 tggtatttcc ttacgatgtg ctctagaatc ttcaggaata ccatgtagat aacgatttgt
    14041 cagcaaccct tgagcaagcg gactgaacgt gattgcccct agcccttctt cttctaatac
    14101 tggagataga tcgtcttcta gtagtgcacg agaaaacata ttgtagcgca tctgatgaat
    14161 aatgaacggt gcttcctttc tttttaaaat ttcagtcatt ttttttgtat cttctccatt
    14221 atagttggat ataccaatat ataatgcttt tccttgtctt acaagttgca tcaaagcttc
    14281 tgctgtttct tccaaaggtg tatcagggtc tgggcgatga tgatagaaga tgtcaacata
    14341 atctagcccc attctttgga gactttggtc acaacttgaa atgatggatt tacgagagcc
    14401 ccattcccca tatggccctt cccacatatg ataacctgct ttgctagaaa taatcagttc
    14461 atcacggtag gcttgaaaat cttctctaaa aatacgccca aagtttttct ctgcagctcc
    14521 agcagggcct ccataattat tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc
    14581 tcttaaaatc tctctttgat tatggatagg atcataatca ccaaaatttt gccataatcc
    14641 gagagataaa atcggtaatt tcaaacctga atgaccggat ttacgatatt ccattttttc
    14701 ataacgtgca ctatctgcaa tatacaattt ttctcctcct catcaaatct ttttaaaacg
    14761 ctttcttttt tctattttta gtttactaca ttttaaagaa aaggagttaa tcaaaatgat
    14821 tttttcgtat atttttttgc catttgtttt ttactcgttg atagatcaga aattgattac
    14881 gtgcttgctg gattttttta taacgtttcg gttgtttttt gtaaaattgc tgatgatagt
    14941 tttctgctgg ccaaaacgta ccggctggtt cgatttttgt gacgattggt tggtggaaag
    15001 ttcctgaact gtctagtttc tgttttgatt gttccgctaa ttctttttgt cgttcgactt
    15061 gataaaaaat cactggacga tattgcttcc ctctatcttg aaattgtcca aatgtatctg
    15121 tgggatcagt aatttgccaa tagattgtca gcaactcgct ataacttatt tctcttgtgt
    15181 cataaatgat ctccacagct tccacatgac ctgtgtaacc accacttact tgatcataat
    15241 ttggtttttc aacatgtccg cccgtgtatc ctgacaaaac agataggatc ccttttttcg
    15301 attcaaacgg ctcgaccata caccagaaac atccccctgc aaatactgca cgttcttgat
    15361 agtcagcatc ttgcatggca tgcttggaaa agtcatattt ttgctcctta tcaaatcctt
    15421 ccgtaatcaa taaataaaag tccattacat caggcgtcag attcattcgg atagctaatg
    15481 gacgtagtgt tgcttctagt tttgataact gttctttaac ggatatattt tctcttatgt
    15541 cattctttgt ctgaatcaat acttttcgtt cccaatctcg tgtattcgga ttcaaaatca
    15601 gattataaag tgtttgaatg aatatttgtt cctcatccat agattctcct cctttccgaa
    15661 gaagacaagc tagtgacttt agctgtaatt tatcataaag tgaggaaata agcgattact
    15721 tcatccccct acagattttt cgtataaaaa agagagaatc cttatcgaca gattctcctt
    15781 tttaatcaat ttattttata aatgaaaagt taatttacct tttatggaaa actattttaa
    15841 aaaataaaca taaatatgca gttaatgtaa caagataaga aataatagtc gttactgtat
    15901 aacttgtgct tactttaata tcagcaactc ctttgactcc gcttatttgt accaatccat
    15961 tagcattcac ctgctccttt acaggttgat gatttatttc tacattcgtt cctaaataag
    16021 catatacagg aagtgtgatt acttggctct tctcattagt tactctatat atgatagaac
    16081 taccagttgt tttataagga aggtttaccc attgactgcc aatctttgct tcgtgtttta
    16141 ttatatgctc cttattctcc aatgaagcga taggagaata atcatataaa aaataatcca
    16201 ttcctttagt ggtatacgcc tctactgttt ctttgtttac aacaatatgt cccggctgag
    16261 aaattatttt ttgattgaaa tttt
//
LOCUS       DAZKMK010000060        16169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000060 DAZKMK010000000
VERSION     DAZKMK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.060"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..426
                     /gene="scm"
                     /locus_tag="ACG8V1_001867"
     CDS             <1..426
                     /gene="scm"
                     /locus_tag="ACG8V1_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0329958.1"
                     /translation="SDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDT
                     SSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKS
                     KRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(581..1567)
                     /locus_tag="ACG8V1_001868"
     CDS             complement(581..1567)
                     /locus_tag="ACG8V1_001868"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0329959.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1590..2156)
                     /locus_tag="ACG8V1_001869"
     CDS             complement(1590..2156)
                     /locus_tag="ACG8V1_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0329960.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2170..2586)
                     /locus_tag="ACG8V1_001870"
     CDS             complement(2170..2586)
                     /locus_tag="ACG8V1_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0329961.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3043..4977)
                     /locus_tag="ACG8V1_001871"
     CDS             complement(3043..4977)
                     /locus_tag="ACG8V1_001871"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0329962.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5279..6214
                     /gene="pplA"
                     /locus_tag="ACG8V1_001872"
     CDS             5279..6214
                     /gene="pplA"
                     /locus_tag="ACG8V1_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0329963.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6304..7407
                     /locus_tag="ACG8V1_001873"
     CDS             6304..7407
                     /locus_tag="ACG8V1_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0329964.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7420..8373
                     /gene="menA"
                     /locus_tag="ACG8V1_001874"
     CDS             7420..8373
                     /gene="menA"
                     /locus_tag="ACG8V1_001874"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0329965.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9187..10533)
                     /gene="gor"
                     /locus_tag="ACG8V1_001875"
     CDS             complement(9187..10533)
                     /gene="gor"
                     /locus_tag="ACG8V1_001875"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0329966.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10667..11920)
                     /locus_tag="ACG8V1_001876"
     CDS             complement(10667..11920)
                     /locus_tag="ACG8V1_001876"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0329967.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11926..12849)
                     /locus_tag="ACG8V1_001877"
     CDS             complement(11926..12849)
                     /locus_tag="ACG8V1_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0329968.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13070..15562)
                     /locus_tag="ACG8V1_001878"
     CDS             complement(13070..15562)
                     /locus_tag="ACG8V1_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0329969.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15569..16036)
                     /locus_tag="ACG8V1_001879"
     CDS             complement(15569..16036)
                     /locus_tag="ACG8V1_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0329970.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4747 a         3378 c         2877 g         5167 t
ORIGIN      
        1 agtgatagca cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt
       61 gatagctcag attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac
      121 agtacagata catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt
      181 acagacacgt cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca
      241 agttcggata acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac
      301 aaatcaaaaa gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca
      361 ggcgttatat tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa
      421 aggtaaaaaa atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt
      481 tttgcttttt atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt
      541 ccttctgaga aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa
      601 tcagttgggt aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct
      661 ttatttcctt caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc
      721 ctcctgtatg ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt
      781 tcttctctaa ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta
      841 aagaatagac cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt
      901 ttgtataatc taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat
      961 ctcctgcttt ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac
     1021 ttaattggaa cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa
     1081 gttcataatt atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac
     1141 tgcgagaatt caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac
     1201 agcagacaaa taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga
     1261 tattaggctg accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg
     1321 ccgtatggag catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg
     1381 gtccaaattg tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga
     1441 tcgtttcttt gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc
     1501 gttttagtgt ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta
     1561 gcttcataaa aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt
     1621 tgtttggttc cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa
     1681 agataattcg ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact
     1741 ggcaagtaca acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg
     1801 acattatgca tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct
     1861 aattgcttga ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg
     1921 aatgtggaaa tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag
     1981 aagctgtctc gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct
     2041 agttttgcac caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg
     2101 atcactcctt gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg
     2161 tgtgaaggtc taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat
     2221 cactaattta tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg
     2281 tacgcagatc tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc
     2341 gatcaagtta taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc
     2401 catcaaaggg aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc
     2461 tgttgcggtg ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa
     2521 aagcaaaata ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag
     2581 gctcattttt gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag
     2641 gttgtgacac ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca
     2701 tgttttctaa ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca
     2761 agaggttgtg acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt
     2821 ttcaagtttt tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt
     2881 ataacaagag gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc
     2941 gggcatctta ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc
     3001 tttctattga aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg
     3061 ggaacttggt ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca
     3121 gcgatccaac ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa
     3181 ccaaatgttt acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt
     3241 tgtcgcactg catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc
     3301 aaagaatacc atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg
     3361 catgatcttt gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta
     3421 tgcataactc aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc
     3481 tccgctggca gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac
     3541 attgtattcc catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa
     3601 ccaagtactt ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa
     3661 ccaaactaaa ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga
     3721 agaatgtcct cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata
     3781 ttggaacata taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt
     3841 cattacgata gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca
     3901 tttggcacca atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc
     3961 gcctttgatc gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg
     4021 aacgatctgc ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat
     4081 atagatgttt ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg
     4141 tccgcgtgct gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt
     4201 ccaaatcagt gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc
     4261 cacttctacg attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc
     4321 agcttttgca gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac
     4381 catcagtgtg atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc
     4441 gatcagttcc ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag
     4501 catggcttta cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat
     4561 atgttcccga atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac
     4621 accaggtgta ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg
     4681 ataggaacct aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc
     4741 tgtgacaagt tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc
     4801 ttctggttct acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga
     4861 atgacgatcg atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt
     4921 tgtagctgaa acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga
     4981 tcatccgctc cttatagact atctatataa tgaatatatg ataattcttt tagtttgata
     5041 cttattacac aatctccata tattatatag atgttctgtt cttttgtcta actccttttg
     5101 gatttttttc aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt
     5161 tgaaatatta catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa
     5221 tgtataatga atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat
     5281 gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg
     5341 cgcttgcggc gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca
     5401 atcttcgact gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt
     5461 gcaagacggc acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt
     5521 tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa
     5581 cggcgaatca aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac
     5641 aaacccagaa aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag
     5701 cggtgtggaa gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca
     5761 acaattgatc caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc
     5821 tgatttgaaa gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt
     5881 gcaattcgaa atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga
     5941 caaagacggt aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag
     6001 cggtacagaa ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg
     6061 cgaagaagat ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca
     6121 ttcattcatc atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac
     6181 aatcgaagta gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg
     6241 gagggtggga cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa
     6301 aagatgcgga aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta
     6361 actggctgca gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata
     6421 ttgaaagatc catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg
     6481 tatgatgaag gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt
     6541 gataaaatca cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg
     6601 atcaaaccag taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat
     6661 agccaagatt ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga
     6721 atcggttttg atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta
     6781 gtggattatc ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa
     6841 ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa
     6901 gtcttgaaaa aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc
     6961 ttaggccaca gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac
     7021 atggctcgcg gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct
     7081 ggaatttacg agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca
     7141 aaaacgggtt atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca
     7201 attgatggtg acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa
     7261 tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa
     7321 gtatatgtga ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg
     7381 atgggtgacc gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta
     7441 gaagtagttg aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta
     7501 ttctctatcg ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga
     7561 atgctagttt ttgatttagc gactaccgct atcaataatt acatggatta tgaaaaggct
     7621 cattctgaag tatataagta tgaagaaaat gtcattggcc gcagaaatct atctcctgta
     7681 ttgattcgaa acatgatttt agctatgatt gcatttgttt ttgttatcgg cgttattcta
     7741 acgatcaata cgggttggct tttacttttg atgggattta tctgttgttt tattggagta
     7801 ttctatacat tcggaccgat tcctttatct cgaatgcctt taggggaaat tttcagtggg
     7861 gtgactatgg gccttggaat ttttgccatg gtcgtttatg tcaatacatt cggttcagat
     7921 gtctatgatc tgacagtcaa cttttcatct ggacaatttg ctttggtagg aaatctttgg
     7981 gctatagcag caatcgtatt agcttcctta cccttggtgt ttacgattgc caatatcatg
     8041 ttggctaata atttgcggga tcttgaccgt gatattgaaa atcatcgcta tacactagtt
     8101 ttttatatcg gacgtcctgc gggaacagtc ttattccagc tattgatgta tgcttgctat
     8161 ttcgtgattt taatggggtt gttgagcggt atctatgaat ggccgatctt ggtcaccttc
     8221 ttgaccttgc caattgtgaa gaagcatttg gatcaattca agcaaacgtt accccagcct
     8281 atcagtttca gctattccat caaaaatatg gtcttattca atgccagtta tgtattgggg
     8341 ctattactaa caatcattat gcaagccatt taacagcatg aagaggttgt gaaaaaactg
     8401 ccctgcatca agtaataaga acagccaaat aaaaatagct ccttatattt ttcgtttggt
     8461 tgggcttatt accgcagatg caagcttttg aacaccgttt atcaggaggt tgtgaaaaag
     8521 tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca tttttgcata
     8581 aatttggctt aataccgaga aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc
     8641 agtgttttgt cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt
     8701 tttttgagct aataccgaag gacagctgat tgaacactat ttgttcggaa aaagtgacta
     8761 actatgacac aatttttttc acagtccctt ttttcacttg atttgattcg taccagcgaa
     8821 cttgacgaga actttcttgg tttggtaact atatggtatt cttttaacat cctcgtttgc
     8881 ttcttctaat ttatttatac aaagtcacac ttttttaaac atctgttctt cgatacattt
     8941 ctatttcttt taaacatcga taccaatatt ttgaacttaa gagtgcatac caaatggatt
     9001 catttactct tcgtcagtta cgaagataat caaaatctcc aacttcccta cttatttcgt
     9061 tttgagaatt atcgaagcaa tcttacataa ataactaaaa aacaaaaaac cagaaaatca
     9121 gtatactgat tttctggttt tacatggttt gttcaataaa aagctgttgg aactgttaat
     9181 attaaattat cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg ctactgtatt
     9241 gtcaaagtct tctttagttg cacccatctt gatagccacg gcaaaacctt gcagcatctc
     9301 atcgacacca acaccaatag catgtaagcc gacgattctt tcttcttttc cgacacagat
     9361 caatttcatt tcgcattttt ggcgataatc atttaatgca aagtacattg gggtaaagcg
     9421 tgaacgataa actttcaatt cattttcgcc atatttttcc aatgcttctt cttctgtcaa
     9481 accaattgtt gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag
     9541 ataagaatct ttctttccat taaataaacg ttctgataaa cgtctaccag ctgcgattgc
     9601 aacaggagtc agatctaatt taccgatcac atcaccaaca gcaaaaatat gttcatccgt
     9661 tgtattttgg tatttatcta ctttgataaa cccttttttg tccaattcca catttgtatt
     9721 ttccagacct aatttttcgg tattcggtac acgtccaccg gcaaaaagta cacaatcacc
     9781 agttaaggtt tcaccatttt caaatagcac cgtatattcg tcattgtttt tagtaatttc
     9841 tttagcaata tgatgtgcgt aagtttttat tccagcttct tggtacattt ggatcaaatt
     9901 gtccgaaagc attttatcaa acgttctaag tggtcgttct tttcgatatg cccacatgac
     9961 ttcactacct aaaccacgaa atacacctga aagttcagcc gcaatgtatc cagcacctaa
    10021 aacgattgct ctcttaggta actctgttaa ttcaaaaaat ccatttgaat caattgcgta
    10081 ttctccacca ggaatggtca ttttacttgc acggcctcca gtagcgatca agattttagg
    10141 agcacgatag gtttcaccgt ttacttcgat cgtttgacta tcgataaaag tagcgtaacc
    10201 ttttatagcc tcgacatgat tgctatctaa tcctctttga taagcaccgt gtagaaaatc
    10261 aatatatttt tctcgtcttt ctactagacc ggcgaaatca acatttttta aatctgcttg
    10321 gataccgtaa cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg cttgccacat
    10381 aactttttta ggtacacaac cgacgttgac acatgttccg cccaattcat tcgcttcaat
    10441 cagcaaaact tttgcgccat gcatccctgc tctgtttgct gaagcaatac cgccagagcc
    10501 gccaccaatt acgatgtaat catatatttg cattttgttc ctccttattg aagagatcaa
    10561 tcaaaacctt tatttaacga tcctttttct acagaaaaca agtgtaacac ttttcttgaa
    10621 ttttgtatgc gaagatgctt aaaacaggga tatatcagct ttttccttat ttacgttttc
    10681 ggatcatatc tccaggtttc accggctgtg cgactggcat agttaaaatc atcattggat
    10741 ttggtgctcg gtcaatggat tcgccttctt cattatgcat aacttcaacg gtttgactga
    10801 aatgactgaa tcctggacca taaaattcga tttcatctcc tacagaaaac aagtttctct
    10861 gacgtatcgt agcaatctgt gttgcctcat cataagataa tacttcaccg acaaatttat
    10921 atactgggat tttacgacgt tcaccgaata attgttcgtt ttcagttggt gtattgtagt
    10981 aaaaacctgt agctaattct cgttgtgcta ctttccataa ctcgtcgatc cattcttgct
    11041 tgcagacata gttttcagga tcttccatat aactgtctac agccgcttta tagacatttg
    11101 acaccgtaga tacataatgg atcgatttca ttcttccttc gatcttgaag ctgtctacgc
    11161 cgttttcaat caattctgga atatgttcga tcattgacat atccactgca ctcatcgaga
    11221 attcttcttc cacttcgcct gtatcagtta aactgtttcg ttcttgacca aaaggcatat
    11281 cgaacaatcc atacttccag cgacaagatt gagaacatcc gccacggttt gcatcccgca
    11341 tcgacatatg gttggacaat gtgcatcgac cagaataaga gatacacatg gcaccatgga
    11401 taaatgcttc gatttcggta tccgtatttt tacgaatctc tgccacttcc tccattgaaa
    11461 cttcacgagc taaaacgaca cgttccaacc cttcttcttt ccagaattcc aatgtctcaa
    11521 aattagttgc tgaagcttga gtggataaat ggatcggcag acctggggct tctgtcgcac
    11581 aaatttcgat caaagctgga tcagaaacaa tcactgcaga aataccgaca tcacgcaaac
    11641 tgcggaaaaa ttcccctgct ccatcttcat ttccttcatg ggtgaccata ttagcagcaa
    11701 cgtatacctt cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt tcttcataag
    11761 taaaattacc tgcacggctt cttaaaccat atgcattccc gccaatatat actgcatctg
    11821 ctccataatg gatcgcggtt tttaactttt ccagtgttcc tgctggcgct aggatttctg
    11881 gtcgtttcaa ttttcttttt actgtcattt ctttcatcat actccttatt tgatagcaga
    11941 aggatcaata taaaagaatc ctgtatctaa ttcacggttt tcaggatgca atttactgat
    12001 ttcgctagtc attttttccg ctttttcttg atcccagcta ccttctaaaa tttgtttttt
    12061 tgcttcggcg aatagtcgag tgatgtttac gaatttttcg cctggagtaa ataatccgtc
    12121 caatttccat gtacagtaat gatgctgata aagttcattc aattcattcg agagattcag
    12181 atcattgctg gcaaaaatat gtgtgccatg actatcttca taaatagaat aatgtgtctc
    12241 ttctttttta ggttccgaga taaaaagacc tctttcacga tctttttgtt cgtcttgttt
    12301 cgtatagtta tagtaattct gtagcagtgg acgtttggat tgatggatgc atgtagcacc
    12361 atacacaagg gtttctacag gaatatcgag tttttcttcc atcgccttca tctcttcaaa
    12421 aggaacttca cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga aattgatttg
    12481 acgtgcgctc gttactaatg tttcagcatc ataaatatac ggtatacgca attcaggatt
    12541 ttttttcatc gtataaataa tacctgtatc accaactgtg atcacatcta cgtttatttc
    12601 ttttaaaaaa gccaaatatt caggtacatt tttcattttt tcaggatgaa tgatcccatt
    12661 tacagcaata ttcgcttttt taccatactt gtgtgctaat tctaccaatt gtcgctgttc
    12721 ttctcgtgta aatgaagcag gcagacgcaa tccaaattgc tcttcaccga aatatagtgt
    12781 atctgcacct gcagcaagca actgttctgc ttgttcaata gtttctactg tggttatgag
    12841 ttcgatcata attttctcct cctacaactt cataatagta gcatagctct aaaaactttc
    12901 caatacttct tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa
    12961 agttcgatat attctgtcca aatataaaaa gagattgtga caaaaatctt gtcacaacct
    13021 cttaatcaag tcttactatt ataaaaattc agctacacag gccaagtaac tatagggaac
    13081 ctactgtttc ctttttaggt ccgcgaacat tcaatgtgat cttacctttt gaagcaccta
    13141 ctgttacgtg gtcacctaaa cgaatttctc cgccaagcaa agcctcgctc aatcgatctt
    13201 caatttcttt ttgaagtgcg cgacggattg gacgtgcacc atattcagga tcaaatccag
    13261 ctttcccgat cacatcgatt gcagatggtg tgattttcac ttgaatatct tgttctttca
    13321 atcgtttgat aatcgctttg ctcatgattt ggacgattgt atggatttca tctttactca
    13381 atgaatggaa gacgacagtc tcatcgattc gattcaagaa ttcaggtcgg aaagctttct
    13441 ttaactcttc taagatacgt ttttgcatgg ctttgtgatc tttagtaaca tcttggacat
    13501 tgaatccgac atttttttct tctcgaattt gtgtcgctcc gatatttgaa gtcataatca
    13561 tgattgtatt tctaaagtcc acttttctac ctttagaatc tgttaaatga ccatcatcca
    13621 atacttgcag aagtaaattg aaaacgtctg gatgtgcttt ctccacttca tctaagagaa
    13681 tcactgaata tggtttttga cgaatttttt ctgtcaactg tccaccttca tcatacccta
    13741 cgtatcctgg aggagacccg attaatcggc ttgtactgta tttttccata aattctgaca
    13801 tatcaacacg gatcagggca tcttcacttc caaacatgac ttctgaaagt gctttggcca
    13861 attctgtttt tccgacacca gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag
    13921 gatctttcaa tccgcttcgt gcacggcgga tcgcacgtga aactgctttg acagcctcat
    13981 cttgaccaac gacacgttca tgcagaagtc cttccagttc taacaagcgt tcgctttctt
    14041 ttttctccaa ctgctgtaga gggacaccag tccactccga tacaactgta gcaacatctt
    14101 cttctgttac acgatcagaa taaccttgct cctgttttgc ttcaattgca gaaacttttt
    14161 gcagcttttt acgtagtgct ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt
    14221 gtttttggat cgctgcttcc ttttcttgta ctagttcatc gatttctagt ttgatcacag
    14281 ttgattttgt taagtgatcg gtttgatcca aacggacttt cgctgctgat tcatcgatca
    14341 aatcaattgc tttatcgggt agttgacgag aagtgatata acgaacagaa agattgactg
    14401 ctgctcgtac agcttcatct gtgatctcca ctccatggtg ctcttcatat cttgtacgta
    14461 atccttgaag aatcacttct gcttcttcag gagttggctc atctacttga atgcgagcaa
    14521 aacgtcgctc taatgcagaa tctttttcga tatatttttg atattcatct agggtcgttg
    14581 ctccgatcgt ttgtaattct cctcgagcta acgccggttt caagatattc gaagcatcaa
    14641 ttgctccttc tgctccacct gcaccaatta aggtatgcaa ttcatcaatg aataagatta
    14701 cttgaccatc attatagatt tcgtcgatga cctttttcat tcgatcttca aattcaccgc
    14761 gatatttcgt accagcaacc aaagatccca tatcaagcat catcaggcgc ttttcttgca
    14821 tgtcttctgg cacttcacct aaaatgattt tttgagctaa cccttcagca atggctgttt
    14881 tgcctacacc aggttcaccc accagtacag ggttattctt tgtccgtcgg ctaagaatct
    14941 ggataagccg tttcacttct ctcgaacgac caacaactgg gtccagcttg ttttcttttg
    15001 ctaatttcgt caaatcacgt gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc
    15061 gtttatcttg tacatttaca tttctacgac gattcattcc ttttccagca gaatcaccag
    15121 ctagccccat tttttgtttc agaacttgac gggcttttga aaggctgata cctaagttga
    15181 tcatgattcg agtagccatg atactttcat cttttaataa gctcatcaaa atatgttctg
    15241 ttccaacttt ttgagaacca gttttcttca cttcgctgct agctaaagca aaaacttgtt
    15301 tcattcttgg tgaatatgga agaaagactc ctttagggta agaacggata ccaccataac
    15361 caatcagatg ttcaatttct tcataaattt ccgtttcatt caaatccatt tcacgtaata
    15421 cttttccagc aattccattt tgctcgacca ctaaggctag taaaacatgt tctgaactaa
    15481 ctgattgatg tttcaaccgt tttgcttctt cttgtgcaat ttctaatact gcttgggctc
    15541 tttcagtaaa aagttcttcc atctaatctc acttcccatc ttcataactt aaacgttcca
    15601 gaaatgctcg gagaatccga gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt
    15661 tttttgcaat tgcactcaac atcaagtttc cttctttttt tgtaattatt tggtcttcat
    15721 aaagtttttg aataatagca aaagcatttt tctcactgat tgtatcatca aataactgat
    15781 ttatctgctc aagtaattgt tttttatctg aaatccttac ttttgcaatt cgtatataac
    15841 caccaccacc acgtttgctt tctactagat aaccacgttg gatggtaaaa cgggtattaa
    15901 tcacatagtt gatttgtgaa ggaacacagt taaagaggtc tgccatttca atccttcgaa
    15961 tctcgatcat ttcactattt tccagaattt tctttatata tgattcaatt aaatccgagg
    16021 tattctgatg tgccatctta ttcattccct ttgactaata ttgaccttaa ttataacgaa
    16081 tttgataata gaaagaaaga agaacgcttt ttgttaaata aatcctttag aatcaacaaa
    16141 aaaaaatacg gaatatacta ttccgtatc
//
LOCUS       DAZKMK010000061        15742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000061 DAZKMK010000000
VERSION     DAZKMK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.061"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG8V1_001880"
     CDS             complement(24..722)
                     /locus_tag="ACG8V1_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0329971.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG8V1_001881"
     CDS             complement(715..2067)
                     /locus_tag="ACG8V1_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0329972.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG8V1_001882"
     CDS             complement(2290..3690)
                     /locus_tag="ACG8V1_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0329973.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG8V1_001883"
     CDS             3851..4588
                     /locus_tag="ACG8V1_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0329974.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG8V1_001884"
     CDS             4713..5192
                     /locus_tag="ACG8V1_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0329975.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG8V1_001885"
     CDS             5189..5956
                     /locus_tag="ACG8V1_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0329976.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8V1_001886"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8V1_001886"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0329977.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG8V1_001887"
     CDS             complement(6859..7461)
                     /locus_tag="ACG8V1_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0329978.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG8V1_001888"
     CDS             7567..7797
                     /locus_tag="ACG8V1_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329979.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG8V1_001889"
     CDS             7788..8036
                     /locus_tag="ACG8V1_001889"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0329980.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG8V1_001890"
     CDS             8142..8726
                     /locus_tag="ACG8V1_001890"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0329981.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8V1_001891"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8V1_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0329982.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8V1_001892"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8V1_001892"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0329983.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG8V1_001893"
     CDS             10627..10806
                     /locus_tag="ACG8V1_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329984.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG8V1_001894"
     CDS             10828..11853
                     /locus_tag="ACG8V1_001894"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0329985.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8V1_001895"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8V1_001895"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0329986.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG8V1_001896"
     CDS             13425..14372
                     /locus_tag="ACG8V1_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0329987.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG8V1_001897"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG8V1_001897"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0329988.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG8V1_001898"
     CDS             complement(15274..15516)
                     /locus_tag="ACG8V1_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329989.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG8V1_001899"
     tRNA            15637..15709
                     /locus_tag="ACG8V1_001899"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tt
//
LOCUS       DAZKMK010000062        15507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000062 DAZKMK010000000
VERSION     DAZKMK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.062"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1016..1357)
                     /locus_tag="ACG8V1_001900"
     CDS             complement(1016..1357)
                     /locus_tag="ACG8V1_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0329990.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="ACG8V1_001901"
     CDS             complement(1357..1587)
                     /locus_tag="ACG8V1_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0329991.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1874..2212
                     /locus_tag="ACG8V1_001902"
     CDS             1874..2212
                     /locus_tag="ACG8V1_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329992.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2417..2979)
                     /locus_tag="ACG8V1_001903"
                     /pseudo
     CDS             complement(2417..2979)
                     /locus_tag="ACG8V1_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3711..4292)
                     /locus_tag="ACG8V1_001904"
     CDS             complement(3711..4292)
                     /locus_tag="ACG8V1_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0329993.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4475..5101)
                     /locus_tag="ACG8V1_001905"
     CDS             complement(4475..5101)
                     /locus_tag="ACG8V1_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0329994.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5123..6454)
                     /locus_tag="ACG8V1_001906"
     CDS             complement(5123..6454)
                     /locus_tag="ACG8V1_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0329995.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6609..7010)
                     /locus_tag="ACG8V1_001907"
     CDS             complement(6609..7010)
                     /locus_tag="ACG8V1_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0329996.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7096..7302)
                     /locus_tag="ACG8V1_001908"
     CDS             complement(7096..7302)
                     /locus_tag="ACG8V1_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0329997.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7397..8899)
                     /locus_tag="ACG8V1_001909"
     CDS             complement(7397..8899)
                     /locus_tag="ACG8V1_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0329998.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8V1_001910"
     CDS             complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8V1_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0329999.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9470..10087)
                     /locus_tag="ACG8V1_001911"
     CDS             complement(9470..10087)
                     /locus_tag="ACG8V1_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0330000.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10308..11681
                     /locus_tag="ACG8V1_001912"
     CDS             10308..11681
                     /locus_tag="ACG8V1_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0330001.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11756..12868
                     /locus_tag="ACG8V1_001913"
     CDS             11756..12868
                     /locus_tag="ACG8V1_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0330002.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12940..14577)
                     /locus_tag="ACG8V1_001914"
     CDS             complement(12940..14577)
                     /locus_tag="ACG8V1_001914"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0330003.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14745..15353)
                     /locus_tag="ACG8V1_001915"
     CDS             complement(14745..15353)
                     /locus_tag="ACG8V1_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0330004.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4306 a         3173 c         2697 g         5331 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     1801 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     1861 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     1921 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     1981 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     2041 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     2101 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     2161 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     2221 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     2281 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     2341 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     2401 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     2461 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     2521 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     2581 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     2641 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     2701 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     2761 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     2821 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     2881 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     2941 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     3001 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     3061 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     3121 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     3181 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     3241 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     3301 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
     3361 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
     3421 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
     3481 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
     3541 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
     3601 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
     3661 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
     3721 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
     3781 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
     3841 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
     3901 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
     3961 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
     4021 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
     4081 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
     4141 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
     4201 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
     4261 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
     4321 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
     4381 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
     4441 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
     4501 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
     4561 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
     4621 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
     4681 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
     4741 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
     4801 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
     4861 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
     4921 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
     4981 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
     5041 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
     5101 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
     5161 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
     5221 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
     5281 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
     5341 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
     5401 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
     5461 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
     5521 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
     5581 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
     5641 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
     5701 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
     5761 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
     5821 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
     5881 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
     5941 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
     6001 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
     6061 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
     6121 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
     6181 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
     6241 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
     6301 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
     6361 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
     6421 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
     6481 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
     6541 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
     6601 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
     6661 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
     6721 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
     6781 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
     6841 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
     6901 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
     6961 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
     7021 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
     7081 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
     7141 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
     7201 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
     7261 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
     7321 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
     7381 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
     7441 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
     7501 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
     7561 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
     7621 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
     7681 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
     7741 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
     7801 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
     7861 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
     7921 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
     7981 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
     8041 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
     8101 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
     8161 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
     8221 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
     8281 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
     8341 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
     8401 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
     8461 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
     8521 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
     8581 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
     8641 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
     8701 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
     8761 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
     8821 agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca
     8881 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
     8941 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
     9001 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
     9061 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
     9121 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
     9181 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
     9241 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
     9301 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
     9361 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
     9421 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
     9481 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
     9541 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
     9601 ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac
     9661 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
     9721 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
     9781 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
     9841 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
     9901 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
     9961 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa
    10021 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
    10081 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
    10141 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
    10201 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
    10261 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
    10321 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
    10381 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
    10441 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
    10501 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
    10561 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
    10621 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
    10681 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
    10741 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
    10801 atcatatctt cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg
    10861 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
    10921 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
    10981 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
    11041 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
    11101 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
    11161 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
    11221 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
    11281 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
    11341 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
    11401 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
    11461 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
    11521 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
    11581 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
    11641 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
    11701 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
    11761 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
    11821 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
    11881 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
    11941 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
    12001 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
    12061 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
    12121 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
    12181 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
    12241 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
    12301 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
    12361 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
    12421 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
    12481 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
    12541 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
    12601 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
    12661 ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt
    12721 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
    12781 attagccaac ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc
    12841 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
    12901 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
    12961 attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat
    13021 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
    13081 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
    13141 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
    13201 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
    13261 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
    13321 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
    13381 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
    13441 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
    13501 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
    13561 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    13621 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    13681 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    13741 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    13801 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    13861 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    13921 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    13981 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    14041 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    14101 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    14161 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    14221 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    14281 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    14341 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    14401 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc
    14461 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    14521 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    14581 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    14641 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    14701 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    14761 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    14821 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    14881 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    14941 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    15001 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    15061 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    15121 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    15181 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    15241 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    15301 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    15361 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
    15421 tcataggtaa aagaaacatg acatttaatg acaaaataac gaattattta atatttgtaa
    15481 catttaaaca aatatttttt tgataca
//
LOCUS       DAZKMK010000063        15314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000063 DAZKMK010000000
VERSION     DAZKMK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.063"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8V1_001916"
     CDS             278..787
                     /locus_tag="ACG8V1_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0330005.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8V1_001917"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8V1_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0330006.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG8V1_001918"
     CDS             2322..3302
                     /locus_tag="ACG8V1_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0330007.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG8V1_001919"
     CDS             3424..4842
                     /locus_tag="ACG8V1_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0330008.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG8V1_001920"
     CDS             4913..6382
                     /locus_tag="ACG8V1_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0330009.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG8V1_001921"
     CDS             6392..6859
                     /locus_tag="ACG8V1_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0330010.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8V1_001922"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8V1_001922"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0330011.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="ACG8V1_001923"
     CDS             7849..10383
                     /locus_tag="ACG8V1_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0330012.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="ACG8V1_001924"
     CDS             10539..11312
                     /locus_tag="ACG8V1_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0330013.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="ACG8V1_001925"
     CDS             11305..12096
                     /locus_tag="ACG8V1_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0330014.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="ACG8V1_001926"
     CDS             12106..12597
                     /locus_tag="ACG8V1_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0330015.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="ACG8V1_001927"
     CDS             12615..13025
                     /locus_tag="ACG8V1_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0330016.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="ACG8V1_001928"
     CDS             13029..14429
                     /locus_tag="ACG8V1_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFQ0330017.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="ACG8V1_001929"
     CDS             14452..14826
                     /locus_tag="ACG8V1_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0330018.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4783 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttct
//
LOCUS       DAZKMK010000064        15260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000064 DAZKMK010000000
VERSION     DAZKMK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.065"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG8V1_001930"
     CDS             2..1021
                     /locus_tag="ACG8V1_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0330019.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG8V1_001931"
     CDS             1018..1785
                     /locus_tag="ACG8V1_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0330020.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8V1_001932"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8V1_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0330021.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG8V1_001933"
     CDS             3750..4070
                     /locus_tag="ACG8V1_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0330022.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG8V1_001934"
     CDS             4070..4420
                     /locus_tag="ACG8V1_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0330023.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8V1_001935"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8V1_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0330024.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8V1_001936"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8V1_001936"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0330025.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG8V1_001937"
     CDS             6414..7439
                     /locus_tag="ACG8V1_001937"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0330026.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG8V1_001938"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG8V1_001938"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0330027.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG8V1_001939"
     CDS             8517..8957
                     /locus_tag="ACG8V1_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0330028.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG8V1_001940"
     CDS             8960..9562
                     /locus_tag="ACG8V1_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0330029.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG8V1_001941"
     CDS             complement(9625..10728)
                     /locus_tag="ACG8V1_001941"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0330030.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8V1_001942"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8V1_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0330031.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG8V1_001943"
     CDS             12294..12689
                     /locus_tag="ACG8V1_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330032.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG8V1_001944"
     CDS             12689..12940
                     /locus_tag="ACG8V1_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330033.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG8V1_001945"
     CDS             12983..13378
                     /locus_tag="ACG8V1_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330034.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG8V1_001946"
     CDS             13375..14631
                     /locus_tag="ACG8V1_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0330035.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG8V1_001947"
     CDS             14643..14966
                     /locus_tag="ACG8V1_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330036.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG8V1_001948"
     CDS             14959..15225
                     /locus_tag="ACG8V1_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330037.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3227 g         4211 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaatggttt
//
LOCUS       DAZKMK010000065        15186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000065 DAZKMK010000000
VERSION     DAZKMK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.066"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..318)
                     /locus_tag="ACG8V1_001949"
     CDS             complement(88..318)
                     /locus_tag="ACG8V1_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330038.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(754..1320)
                     /locus_tag="ACG8V1_001950"
     CDS             complement(754..1320)
                     /locus_tag="ACG8V1_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0330039.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1320..2276)
                     /locus_tag="ACG8V1_001951"
     CDS             complement(1320..2276)
                     /locus_tag="ACG8V1_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0330040.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2276..2932)
                     /locus_tag="ACG8V1_001952"
     CDS             complement(2276..2932)
                     /locus_tag="ACG8V1_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0330041.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3302..5320
                     /locus_tag="ACG8V1_001953"
     CDS             3302..5320
                     /locus_tag="ACG8V1_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0330042.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5390..5596
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5747..8161
                     /gene="leuS"
                     /locus_tag="ACG8V1_001954"
     CDS             5747..8161
                     /gene="leuS"
                     /locus_tag="ACG8V1_001954"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0330043.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8509..10062
                     /locus_tag="ACG8V1_001955"
     CDS             8509..10062
                     /locus_tag="ACG8V1_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0330044.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10443..12227)
                     /locus_tag="ACG8V1_001956"
     CDS             complement(10443..12227)
                     /locus_tag="ACG8V1_001956"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330045.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12242..13963)
                     /locus_tag="ACG8V1_001957"
     CDS             complement(12242..13963)
                     /locus_tag="ACG8V1_001957"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330046.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            14063..14824
                     /locus_tag="ACG8V1_001958"
     CDS             14063..14824
                     /locus_tag="ACG8V1_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0330047.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4942 a         2789 c         2983 g         4472 t
ORIGIN      
        1 aatcccacac cttataaaaa tctttgacaa atctattcga aggatcaaat ttaaaccata
       61 attgcgtctc attaaaaagc ataaccatta ctccgatcta tttttaaact tagtctctac
      121 ctctaatata tatcgagttt ttattttgcc ttcagagaat actgtttctt tttttgcagt
      181 tacaggttgt ttattttcgg aaaaagctaa tatagctaaa attgaaacat ccatctggaa
      241 tttatctttt ttactgcttt gatcataaaa gtctgcatat tcatcactga tattttttct
      301 aataaattct tccatcataa aaatcacctc aaaacgatta tacgaactta cgttcttgtt
      361 gtaaagcgat atttgagggg caaaaaaggg gcataatttg actgatatta agttagctat
      421 tgatatatga atgacataca aaaatataaa atcttttaaa aatgcttata tatcaatgtt
      481 tttgccattc ttttatatat ctgttatatg tcaaaaaaat gtgttcaatt tcaatttgta
      541 tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaatcaa
      601 aaacagcttc tcatgttttt gatttggttg gacttattac cgcagatata agcttttgag
      661 caccgtgaat aaagaggtta tgaaatcaac gctctaacct gagtattaga gaaaaaaacg
      721 caaaacatat catttatgtt ttgcgttttt tatttatctt tttttctcaa tacaatataa
      781 aataggcggc tggttctttt ggttgataaa ccgataattc agcacattat atgtttcctg
      841 tggcagtttg gaacaaaaag aatcaaccat atccagttct ttttctccac cttcatgacc
      901 atagtagacg acaaggatca atcgtccgcg aggcacaagt cgtttcaata tttcttccat
      961 agctgtgcgt gtagtctctg gcatagtgat gattgcttta tcactttttg ggagataccc
     1021 tagattgaaa atccctgctt tgagattttg ttgttcagct atcacttctc ctaacgtttc
     1081 gtggccttgt agaaacaatg agacacgttc ttctaactga cgctgttcta gcctttcacg
     1141 agtagcatcg attgcttgtt gctggatatc aaaactatag acatgaccag tttttccgat
     1201 gtgttctgct agaaaaaccg tatcgtggcc attgcccatc gttgcatcta cgacgtgatc
     1261 ccctggttgc aagatttctt gcagtaatgt atggctgaaa tgcaaagcag tttgcaacat
     1321 cagacgctca cctttttccg tacatcatat tttccttgga agctatctct gcgaagaagt
     1381 tcttcatcaa ttgcattcaa gacttcccat tttttcaagc tccacattgg tccaatcaag
     1441 gaatcccacg gcgcatcccc tgtcaaacga tggatgatga tctcttttgg tatcatttcc
     1501 agctggtcgc aaatgacatt gacatagtca ggacggctca ttaattgaag gcgaccttca
     1561 tgatagtccc gcaacattct cgttttgcgc atcagatgca tcaaatggag cttgattcct
     1621 tggatatctg aatctaaaat ggtgcgtctg acattttctt tcatcatttc taaactttcg
     1681 cctggtaatc cgttgatcaa atgcgtacaa acacggatat tatgtttgcg taatttagcc
     1741 acgccatcta aataagtttg ataatcatgt gctcggttga tcagttttga agtttcttca
     1801 aaagttgttt gcaatccaag ttctacccat aaatacatcc gctcgttcag ttcagcaagg
     1861 taatcaacta cttcatctgg cagacaatcc ggtcgtgtac cgactgataa tcctacgaca
     1921 cctggtaaat tgactacttg ctcaaatcgt tctttgatta tttcaactgg cgcatgggtg
     1981 tttgtaaaat tttggaaata aacgatgtat tgttggacat gaggccattt ttgatgcatc
     2041 atgtcgattt ctttctggaa ttggattgga agagggtctt caggtgcaac gatcatgtct
     2101 cccgaaccag atacactgca aaaagtacaa ccgccatggg caacagtccc atctcgattc
     2161 ggacagtcaa atcctccatc gatcggcacc ttgaaaattt tcccgccaaa ctcatttcgt
     2221 aaagcgtaat tccaagaatg atatctttta tttccttctg aataaggaaa agtcattaaa
     2281 agtccttctt tctgtaagag ttatctattt gttttctttt ttctctatac actggataag
     2341 tcaggcacag ccaagaaaga ctgagtaagt aaccgccgat gatatcagta ggaaaatgaa
     2401 cgcccagata aatgcggctt atcccgacac ttactataat aaacatcatc agcaattgaa
     2461 gcaatgtgcg taaggttctt ttttgaacaa aagctggaag cagaaatagt aaagaaccgt
     2521 aaagaatcat actgcctgct gcatgtccac tagggaaact gtaacttgtt tcaactacta
     2581 agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt gatcaccggg ttgatgattc
     2641 cagcaattac aataaatcca gaaacgaacc aaaatgtttc tgcccttttt ttcttatacc
     2701 acatccataa agccgcacaa gcaaataaaa tgatgacggt aagaggatta ccgaatttag
     2761 tcataaagag ttggtagctg ttcagatcag gataagaagc tctgacaatg cctgtaaacc
     2821 actgatcgaa accagaaagc cacgacagat ggaactttac gatccagccc aaaatgaaaa
     2881 acaggacaag aaagaagcta ccagccacct gaaatgatgt tttatatttc atacaattcc
     2941 tcctttgaag caatgactta ttatagcatt tttggtccag cgaaaaaaac ggtaaagaga
     3001 taaaaccaat gtatgattcc tctttgaata accaatattc ttataaaaaa aataactgta
     3061 atcggaaaga cattttttgg taatgcaaat gaaatgttag ggaaataaaa tcagtgtttt
     3121 tcgaaatcct tttaaacaag aacgtagacg taggaagaac ttgaaaatga acagaagttg
     3181 ttcattacgg taaattacaa tatgattaca aaaaacaaca aagctcttaa ataactttta
     3241 ataaatatta agagaagtta taattattaa taatagataa attgaagttg gaggatagaa
     3301 gatggaaaac attgcaagaa aagaacggag acgtttagaa cagaccaaac gttttcgaaa
     3361 agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta ggctgttctg tcgctgcccc
     3421 attagtacag ccggttcctg ttcaagcgga tgaaacacct gttcagttcg gcgcaagaat
     3481 caatacagcg gcatttatca cagaaattgc tacttatgct caaccaatcg ctcaagccaa
     3541 cgatctatat gcatcagtga tgatcgcaca agcagttgta gaaagcggtt ggggcagtag
     3601 tgcgttatct caagcacctt attataatct ttttgggata aaaggcagct atcaaggaca
     3661 aaccgtctac atggatacgc ttgaattttt aaataatcaa tgggtaaata aaaaggagcc
     3721 tttccgacaa tatccttctt ttgctgaatc tttcaatgac aatgcctatg tacttagaaa
     3781 tacttcattt ggcaacggtt attactatgc cggtacttgg aagagcaaca cgaaatccta
     3841 tacagatgcg acagcttgtt tgacagggcg ttatgcgact gatcctggtt acgcaggcaa
     3901 attaaataat atcatcgcaa cttacggatt gacccaatat gatacaccag ctagcggaaa
     3961 cgcaggcggt ggagcgacta caggtggcaa taatactgga agtgcaggta gtactggcgg
     4021 ttcaagcaat tctggtaata caaactcagg ttcagcaact tcgaccactt atactgtcca
     4081 atctggtgat tctgtttggg gaatctcaaa taaattcggt atcacaatgg atcaattgat
     4141 ccaatggaac aacatccaaa acaatttcat ttatccagga caaaaactga caatcaaagg
     4201 tagccaggaa aatggctctt cgacgaataa ttccggtaac aataccaatt cttcaggaaa
     4261 tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa tacactgttc aatctggtga
     4321 ttctgtttgg aagatctcaa atgatcacgg aattacgatg gctcaattga ttgaatggaa
     4381 caatatcaag aacaatttcg tttatccagg acaacagcta atcgtatcaa atggcggttc
     4441 ttctaatgct ggagcaaata ctggctcaaa tactgggaat acaagtggct ctagttcgtc
     4501 cagctcaagt tcttctaata cagcttcagg aacgaaatat acagttaaag ccggagactc
     4561 tgtttggagc gtctcaaata aatacggaat tacgatggct cagctaatcc aatggaacaa
     4621 tatccaaaat aactttatct acccaggaca acaactgact gtttcaaaca gtggatcaac
     4681 aaacagcacg tcaggtaata caggttcgtc aaccaataca aataacagca gttcagttaa
     4741 tactggcagc agttcgtctc aaggcactta taccgtgaaa gcaggtgaat ctgtttggag
     4801 cgtggcaaat aaaaacggca tcacgatgga tcagttaatc gaatggaata atatcaaaaa
     4861 caacttcatt tatccaggac agcaattgat cgttaaaggt ggaacttcgg caaatacaaa
     4921 tacaggttca actacttctg cagcgaaacc aaatacaccg aatacgtctg ctacgacaag
     4981 tacatcatcc actggtaata cgatgtatac cgtgaaagcg ggcgagtcag tttggagcgt
     5041 agcgaacaaa catcatatca cgatggatca gttaatcgaa tggaataata tcaaaaataa
     5101 tttcatctat ccaggacagc aagtgattgt gaagaaaggt tcatctcaat caacaaacca
     5161 acaatcagtt actggttcga aaacctatac tgtgaaatct ggagaatctg tttggagtgt
     5221 tgctgacagt catggaatta caatggctca actaatcgaa tggaacaata ttaagaacaa
     5281 ctttatttat ccaggtcaaa cgttgattgt agctaaataa aatagctgtt cgaagaaata
     5341 acgattgaat tccacaaaaa tatttgttac tataaggcta cttaaatatg acaacacttg
     5401 aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca tggctggtga gaatgtgcac
     5461 ggatgctttt gaactcgcct ttgagttgcc cttgctgaat acccgagtaa gcaagcggcg
     5521 gtgacggccg ttacaacgtt cgaggccttt tcactaaggt aaaattaggt ggtaccgcgt
     5581 agattacgtc ctataaaaag agcaatcttt ttataggatt tttttatttc ttttgtgaag
     5641 tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat tctgccggtt ttttcgttat
     5701 atttataata gaaacaagtg ttttcaatag aacaggagga cagcaaatga gttacaatca
     5761 taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa acgaatactt tcaacaccca
     5821 tgatgatcct gaaaagccaa aattttacgc actggatatg tttccctatc catccggtca
     5881 agggctacat gtagggcatc ctgaaggtta tacggcaacg gatatccttt cacgtgtgaa
     5941 aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg gatgcattcg gtctgcctgc
     6001 tgaacaatat gcgctagata caggaaacga tccagctgag tttacccaaa aaaatatcga
     6061 aacattccga cgccaaatca attctttagg atttagttat gactggaatc gcgaagtgaa
     6121 tacaactgat ccggagtatt ataaatggac acaatggatc tttacgaagc tttatgaaaa
     6181 aggtctagct tatgaagcag aagtagcggt aaactgggta cctgaattag gaacagttat
     6241 ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt ggctatgatg tagtcagaaa
     6301 accaatgcgc caatggatgc tgaaaatcac tgcttatgct gatcgcttat tggatgatct
     6361 agaattagtt gattggccag aaagcattaa agaaatgcaa agaaattgga tcggtcgttc
     6421 agtaggagca aatattgaat ttaaagtggc tggtacggat aaatcctata ccgtatttac
     6481 gacacgacca gatactctat tcggtgcaac ttactcggtt cttgcacctg aactagatct
     6541 tgttagagaa attacgaccc ctgaacaaaa agctgctgta gaagcttata tcgaagagac
     6601 agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa gaaaaaacag gagtatttac
     6661 tggtgcttat gcaatcaatc cagttaatgg caaggaaatc ccaatctgga tcgccgatta
     6721 tgtattatct tcatacggca caggagccat catggctgtt ccagcacatg atgaacgtga
     6781 ctttgaattc gctcaaactt tcggactaga aattcttcca gttatcgaag gtggagatgt
     6841 tgaaaaagct gcttacacag aagatggtac gcacatcaac tctgaatttc tagacggcat
     6901 gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta gaagaaaatg gcgtagggaa
     6961 aaaagaagtc agctatcgct tacgtgactg gctattctcc cgtcaacgtt actggggtga
     7021 accaatccca atcatccatt gggaggacgg aacagtcaca gctgtcccag aagaagagct
     7081 accattacgg ttgcctaaga caaaaaacat caagccaagc ggtactggag aatctccatt
     7141 agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta acaggtaaaa aaggccgtcg
     7201 cgaaacgaat acaatgccac aatgggcagg aagctcatgg tattacctgc gttatatcga
     7261 ccctcataac aaaaaagaac tagcagatta tgaaaagttg aagcgttggc taccagttga
     7321 catctacatt ggtggagcag aacatgcggt acttcattta ctatatgcac gattctggca
     7381 taaattctta tacgatatcg gtgttgttcc tacaaaagaa ccattccaaa aactatacaa
     7441 tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca aaatctcgtg gtaatgtagt
     7501 caatcctgat gatgtggtcg aaacttacgg tgcagataca ctgcgcatgt acgaaatgtt
     7561 catggggcca ttagatgctt cgattgcttg gagcgaaaat ggcttagaag gaagccgtaa
     7621 attcttggat cgtgtatggc gtttgatcgt tgatgagaac aataaaatgc gtgaccgtat
     7681 cactacattg aatgatggca agcttgataa agtgtatcat caaacagtga aaaaagtgac
     7741 agaagactat gaaaatcttc atttcaatac agcaatctcc caattgatgg tctttatcaa
     7801 tgaagcctat aaagtggacg ctctgcctta tgaatatatc gaaggatttg ttcaactact
     7861 cgcaccgatt gctcctcaca tgggtgaaga actatgggca atccttggaa atgatggtgg
     7921 tatttcttac gcaccatggc caacatacga tgaagcagca cttgttgaag acgaagtaga
     7981 agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc aatgttcccc gagacttagg
     8041 aaaagatgag ctagaaaaag cagcattagc caatgaaata gtgcaagaat atattgaagg
     8101 caaaacagtc agaaaagtta ttgcggtacc aaacaagtta gtcaatatcg ttgctaatta
     8161 attggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctgg gccagaatga
     8221 acttcattct gtctcagatt ttttgttatt tcaaattgat taattgattg atcaaaaagg
     8281 ttttgatttt catgttctca aacacgttca atgtcttttt cttctgaatg gtcttgtaag
     8341 agatctctgt aatcatcaaa tttttgtcta ttaatcttag tattttttga tgtataaact
     8401 acctgaaatt gagagtacat accaaattca taaatttttt attagtggga ctagtgattg
     8461 tatggatttt gacgcataat gaatgaagta tgagaaaagg agagacaaat ggatcaagaa
     8521 ctatttatta tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc gaaaaaaaga
     8581 gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt gctttttttc
     8641 ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc tgaagggatg
     8701 caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac aattgttgtg
     8761 cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg tcatttcgat
     8821 caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc tcaagatgat
     8881 gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca aaaaagtgat
     8941 tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga aagcaaatca
     9001 aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag atgggaaaaa
     9061 agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt cactggtttt
     9121 ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc aaaaatgtct
     9181 tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac gcatatacaa
     9241 aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac aacacaaaag
     9301 ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac agatagtggt
     9361 gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga atacaccctg
     9421 acaacggggc tgattcctga aggttattgg atggacaaca catatgatat ttccgccgat
     9481 ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc aagagataat
     9541 ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg ttatcgaaaa
     9601 gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg gatgtctgca
     9661 gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa aagtggaagt
     9721 acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt aggttttgtt
     9781 tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa agaatcagtt
     9841 ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc accaaatgaa
     9901 gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa aaaaggacca
     9961 atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg ggaaacatgg
    10021 caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga gattgtgagc
    10081 ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc atatttgttg
    10141 aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt tgaagtatag
    10201 cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg aacaccgttt
    10261 attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga aactgaagga
    10321 gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc actagtacat
    10381 ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt agataatgtt
    10441 tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg tgattggagt
    10501 agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag gatcatgtct
    10561 gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc ttttgtcaca
    10621 gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga agttgcttca
    10681 tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa taattgttgc
    10741 tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc gtctggcagt
    10801 gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg ttctcttgtg
    10861 gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag ccaggtattt
    10921 tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt aatatcaaga
    10981 ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag attgactaaa
    11041 gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg ctttactgaa
    11101 aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac atttttgaaa
    11161 gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg aatttcatct
    11221 gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg gatcgagttg
    11281 atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg aagataagct
    11341 tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa taccgcactt
    11401 atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaca gttcataaat
    11461 tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc agcttctgca
    11521 tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc ttccattcga
    11581 ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt tttgttcgca
    11641 atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa agtaagcttg
    11701 ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga agtaaaaaat
    11761 tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt tgtccgacta
    11821 atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt gaattttttt
    11881 gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat tgtccgttcc
    11941 tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa gacagggaac
    12001 aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt tcttatttct
    12061 gataagagat catcgatccc gattcccatg atcaagggca tggctacgac taataggttc
    12121 ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt gatcattttc
    12181 caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt atcccttctt
    12241 tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac agtagttctt
    12301 catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta tctgcttgtt
    12361 ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg tgtttatgaa
    12421 gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta tcatcgaata
    12481 catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt ttctggcccc
    12541 ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga agggtgtcca
    12601 ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt gaggcatatt
    12661 catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc ttctgaggga
    12721 cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga ataccaccaa
    12781 gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc aaactacttt
    12841 ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa
    12901 tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt tcaaaagcag
    12961 ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct tcctcaattt
    13021 cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat ttagggataa
    13081 tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag tattcgacta
    13141 cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg ccaaaccaca
    13201 aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc caagcaaata
    13261 ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc gttcgctttt
    13321 cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag aattctctca
    13381 atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt gcccctcgtt
    13441 tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta acccatgtta
    13501 gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc ggtgctggta
    13561 agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca ttcgtcattc
    13621 ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc tgttggaatt
    13681 tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat gctgaaaagt
    13741 aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt tcaactccaa
    13801 ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc tttgctacta
    13861 attttggtac acctaatgta ccggcgattt ctaaacagat catcacaaag gtaaggagag
    13921 cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc ctcctttttt
    13981 cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa aaaagaataa
    14041 actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag tatcggaact
    14101 atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa tccgttcaag
    14161 tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta tgctttatga
    14221 tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt tatatggaaa
    14281 aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc ataaagaact
    14341 ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt tgaaattgat
    14401 gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact gtatgatctc
    14461 aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt caagctttgg
    14521 gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta tcgaccctag
    14581 tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg
    14641 tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt tagaaatcag
    14701 agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt atctattgac
    14761 gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag aaatgaaaaa
    14821 ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta taaagaaaaa
    14881 ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata gatagaattt
    14941 tagtttttta gaggaaatat gtctttaaaa atggaatggt tgcaattctt agcgatatat
    15001 acgatgtaat tgtcctctta aaaaagtatg gaaaaataat tgtattgggt gttatgtata
    15061 attcatggtg aactgagact aatgagaaga aaaattcata ttgttatgag tgagattgta
    15121 tcagatgaga atgataaatt aaataggtgc ataaaagcat tacacttaaa atacattgtt
    15181 gcttac
//
LOCUS       DAZKMK010000066        15167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000066 DAZKMK010000000
VERSION     DAZKMK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.067"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACG8V1_001959"
     CDS             <1..730
                     /locus_tag="ACG8V1_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0330048.1"
                     /translation="LFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRL
                     VKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFI
                     LVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTIL
                     TILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQS
                     VVGFVLLMITNTIARRFDSDSALF"
     gene            742..1662
                     /locus_tag="ACG8V1_001960"
     CDS             742..1662
                     /locus_tag="ACG8V1_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0330049.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1703..3160
                     /locus_tag="ACG8V1_001961"
     CDS             1703..3160
                     /locus_tag="ACG8V1_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0330050.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3505..4119
                     /locus_tag="ACG8V1_001962"
     CDS             3505..4119
                     /locus_tag="ACG8V1_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0330051.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4112..5836
                     /locus_tag="ACG8V1_001963"
     CDS             4112..5836
                     /locus_tag="ACG8V1_001963"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0330052.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5833..7284
                     /locus_tag="ACG8V1_001964"
     CDS             5833..7284
                     /locus_tag="ACG8V1_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0330053.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7757..9910)
                     /locus_tag="ACG8V1_001965"
     CDS             complement(7757..9910)
                     /locus_tag="ACG8V1_001965"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0330054.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9912..10958)
                     /locus_tag="ACG8V1_001966"
     CDS             complement(9912..10958)
                     /locus_tag="ACG8V1_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0330055.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11108..12397
                     /locus_tag="ACG8V1_001967"
     CDS             11108..12397
                     /locus_tag="ACG8V1_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0330056.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12410..15112
                     /locus_tag="ACG8V1_001968"
     CDS             12410..15112
                     /locus_tag="ACG8V1_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0330057.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         5109 a         2648 c         3052 g         4358 t
ORIGIN      
        1 tttattttct tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc
       61 atttatcctt ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag
      121 aaataaacga ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg
      181 ggtcattatc agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca
      241 gtggatcact ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc
      301 gtttatcttg gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt
      361 tgcatctgtt atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag
      421 caaatggcag cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt
      481 aacgatttta gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc
      541 taggaattcc ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg
      601 cctgacatcc acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt
      661 cggctttgtc ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc
      721 attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct
      781 ttcaataaga cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt
      841 attttgccat ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact
      901 cacgggtaca gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac
      961 cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt
     1021 acactgatca atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt
     1081 ccattccgtc gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg
     1141 gtggccaatt atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg
     1201 atcttgccaa tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt
     1261 tcggtctctg attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata
     1321 tttgttcaga tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc
     1381 gcattgggtt actggaatga ctggttcaat gctttactat acatccaaga tgataagcta
     1441 gttccactac agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat
     1501 tcaggagcag gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga
     1561 atggcgatcg tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag
     1621 cattttgtca aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat
     1681 cataaaatta ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc
     1741 agcgttgttg ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga
     1801 agctagcaat tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga
     1861 tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat
     1921 caattacatt ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga
     1981 aaattatgat atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc
     2041 tgatttgact gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc
     2101 atatattaaa ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt
     2161 ctttgctcaa caagtactta cttttaacaa agctttacta gataaatacg atctttcaat
     2221 tgatggaatc caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga
     2281 accaaacacg gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta
     2341 tcctttaggg aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat
     2401 caaccaatac gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa
     2461 acaaggactc attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa
     2521 tacttggcta atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac
     2581 aaatgcagca aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc
     2641 tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt
     2701 agaacttcta gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat
     2761 cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg
     2821 ttatcagcca aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac
     2881 acaagagtct attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga
     2941 aacttcaccg attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa
     3001 tatttctaat gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga
     3061 tgaaacatta ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa
     3121 agaaatgcaa aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg
     3181 gttgtgacaa aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt
     3241 ttctcggtat taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc
     3301 ttactactcg aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct
     3361 aaccctcggt attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc
     3421 tctaataatc aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata
     3481 aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg
     3541 atctgggtca ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc
     3601 atctttggat taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc
     3661 gagtataaag aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga
     3721 ggcaatctgc tatttagttt atttttatct ggtactgctt tattgagtta caatttatat
     3781 ttatctgttc aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc
     3841 ttgctctgcc tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc
     3901 tctgttccta atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt
     3961 ctaaaaataa ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg
     4021 atcctgtttg gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg
     4081 cgtgaagaat tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa
     4141 aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat
     4201 gactgctacc gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa
     4261 catgacaagt gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag
     4321 taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta
     4381 cttaacaaaa cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga
     4441 tgaattagtt tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag
     4501 tgcgatatat atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa
     4561 aggtggggaa ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc
     4621 tatcacacaa ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact
     4681 agatatgatt ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg
     4741 aacgacaaat cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag
     4801 acaagaaatg atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct
     4861 ggaaaaaaac aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt
     4921 actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg
     4981 tatcattatt ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc
     5041 acagcaagta tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac
     5101 gcggatcgac ataacaaaca cggaatatga actgaaggaa ctttcaatcg gtatcaacga
     5161 aatgttggat agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca
     5221 agacgcacat atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt
     5281 agaatatatc cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta
     5341 tgcgttttcc gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga
     5401 agaactggac ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt
     5461 agcctatcat tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat
     5521 ccagccatta gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc
     5581 gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg
     5641 aaaagggatg actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt
     5701 ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg
     5761 cagcagttat tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac
     5821 attcaaggag ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg
     5881 aatgggttaa aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca
     5941 gagaatgcca aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg
     6001 gatgtgacaa tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt
     6061 cataattttg aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt
     6121 atgcaattag gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa
     6181 agcctcaaga aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa
     6241 atctatcaag aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa
     6301 gaagaaataa taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca
     6361 agaactcatg atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat
     6421 caacgaaatt acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat
     6481 ggcttctgtt gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg
     6541 gaagagacag attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta
     6601 cagttgcatc aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa
     6661 acaaaagatc aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaatta
     6721 gaagtcgctc aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg
     6781 ccggaagata tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt
     6841 gatctggaag atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg
     6901 caagaacttc agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac
     6961 agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca
     7021 ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa
     7081 aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa
     7141 caactgcttt tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac
     7201 aacaccacgt atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac
     7261 cgagaaaaca gtggagaatc ataaaagaaa aagccaatgt aatgaaataa cttataaaaa
     7321 attgtctaga aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat
     7381 gacctctcga taaacggagt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
     7441 aaatgtggaa agccattttc acaaaatttt tcttaatact cgaaactaaa cagctttttc
     7501 acgacctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg
     7561 caaaatatga ggaactattt tgccatttgt ctctctaata ctcaggtcag aacgctgatt
     7621 tcacaacctc tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa gaccaaccaa
     7681 acgaaaaata tgaggatcta tttttgcttg gctgttctta ttacttgatg caggacagct
     7741 ttttcacaac ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct tttgcttgat
     7801 atttcttagg gtgagggatg atccctaatt ggtaagagat cgtgccgcct ttcagcaaat
     7861 cttgatgggt aaaatataat ttcgtatatt ctttgtccat ccgtttgatt ttatggataa
     7921 attgttgctg cggccgatta ggcgttgctt caatcgtcaa ttcctttcca tttgacaaaa
     7981 gcaaaacagc cttgtcaatt aatggcatac cgataacata ttcaccggtt ccaggcgtga
     8041 caggataaaa tcccagacta ttgaagatat accaagcggc catacttcca ttgtcctcat
     8101 ctccagggta acccattgga gaatcgtcga aggtctgagt catcaactgt ttaagcagtg
     8161 gctgtgccat ttcaggttgt cctatataac tgaacaagta aggatagtga aaacttggct
     8221 ggtttgaaat agctaattgt ccaaattcta tcgcggccat ctcactcatt tcgtggatct
     8281 caaaaccata tcctcctggg tcaaacgttg ggggctgatt gcacagctca atgagttttt
     8341 ctttatatct ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg tgataaacag
     8401 caaagctgct ttgccaagca cttccttctg cataatcttg tccccaacga ttagctgaaa
     8461 atggttctct gaacttgcca tctttatctt ttgaacgcat aaatcctgac tcatagtcaa
     8521 agatattttg atagttcaat gattgctgct gatatttctt cgtcatttcc tcttcttcta
     8581 gtaccttggc aacttggcta atacaaaaat cgctataagc atagtctaat gtgtgattga
     8641 ctgattcgtg gtagtgatta ggaacatagc catatgtcaa gtaatcttct gttccttggc
     8701 gtccataatt tggatgtttg ctttgaaccg tagcaccttt ttccattgcc gttagaaatt
     8761 caggcatcaa gtctcgtcgg atatctttta cagcagcatc tgcaatgacc gcatcgatca
     8821 atgtcccagg cataagtcca cgttcatcag gagacagcca ttttggcaaa tagccacttt
     8881 ctctatagct gttcaaaaaa ccttccagca tttcttcgta tttttctaca gcaatcaaag
     8941 aataaagagg ataaaccgtt ttatacgtat cccaaaaccc attatttgta tagagaacac
     9001 caggttttat agatcgacta gtcgtatcat agtggatttt ttgcccacta ggatcaattt
     9061 cataaaatgt ttgtggaaat aaaaacaccc gatataaatt atgataaaac gtccgcaatt
     9121 gttgttgatc atggtgtgtg agctgtatac gatcaaaata ggactgccat tgttcttgac
     9181 tgtgagcaag gtaagtctct ttgctttggt tttgttctag ttccaggtta taagctgctt
     9241 gttccgagct gataaaagat gtcccaaatc ggatagtttg tttttttgtc tgttcaaaat
     9301 acaacgggat cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt gttaaaggat
     9361 attcaaatct caatataaaa taaaaagaaa aatcaggatc ttctgaacca gcaaaatgtg
     9421 tgatttttcc ttccactgtg taaggatcta taacaaagag ctgatggcgt cccggaaagg
     9481 ataaaagtaa accgctgtca tttttgctgt aatcaataga taagattccg ccatacatgc
     9541 taggtatcaa tgtagaacag atgccataac gtaattgaca gatcgacaga tgagtcgggc
     9601 aaaatatact ttcttctggg cggtaagaac tttgagcatg gaaaagggtg ttctcctgta
     9661 gttttccatt gatcggcgta aacagcatat gactgaaatc acccatccaa ggacttggtt
     9721 gatgggtaag acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac cagctgccgt
     9781 tttgatccgt agtttgcggt gcaaaataat tcatcccaaa aggaacgcca gtataaggct
     9841 ggcagttccc attagagaaa tttggctgat tcgctgttgc atgtctagta tctatttgac
     9901 cgatattcat tttatttctc ctttgttgat tgtcttttga tgaatgaaac tggaaattta
     9961 ctatcggatg gtcgatcttt tgtctctaac caagtgacca aagcatctcc cgcaagctta
    10021 cctaactgtt caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag agctgctgct
    10081 tggatatcat caaatccgat aattgaaaaa tcttcaggga tatgataacc atgctgtctc
    10141 aagagattca tggtctggat agcgacaaca tcgttttcac aaacaaaagc agttacttca
    10201 tacttagtga tataagaaag caattcatct aatgtcgcgc ttttgtcatc cagtgaagtg
    10261 tggaaagtca aaccagcttt gtccaatgct tcaaggtaac caaggtaacg ctgtcgaacg
    10321 gattgtggat gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc ctgttcgatc
    10381 aaataagagg cagcaagcgc accgccttgg aaattatcgg aaagaacggt aggaaaaggt
    10441 aactcataga tttttttatc taatatgacg acagggaatt ctttcaatga aagctcaaaa
    10501 agtaagtcaa gatgttgttc tgtatcaaga gcataataaa tcaatccgtc aaattcattg
    10561 attagctctt ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat gacatcaatc
    10621 tgtttatctt gcatcacagg gttcaatccc ttgctaaaat caccaacaga caaatcagcg
    10681 acatatggaa gaagaaacag aatccgtcgt gtttttgtag gagaagcaag aatctgttcc
    10741 ggagaagcgg atttaacgaa actgcctttc ccgcgaacgc gataaataag tcctaattgc
    10801 tctaattcag tcaatgcacg tttggaagtg attctgctta ctttataggt acgtgaaagt
    10861 tctttttcag ttggtagttg gctgtctaca ggtaattcac cagttaaaat agccgtttgc
    10921 aaatcattaa atattgtctg gtacaatggt tgattcatat aaacacctct taatgatata
    10981 tcatattcaa ataataccat aatggatgaa aaagtaaata aaaagatatt ttttgaaagc
    11041 gtagacataa ttggctgtat gaagtataat ctttaaagat atatcaaata gaaagaaggt
    11101 tttattagtg gcttataaag atataccaaa atcggtgcag aaatttatgg aagatatcac
    11161 acaaaaatgt ggggatatcc atgcggactg ggcaaagaat tttacagcag catttgccaa
    11221 tactctgttg acaacagtca aaaagcaaaa tgacggaacc acctttttat tgacgggaga
    11281 cattccagcg atgtggttaa gagactctac tgcacaagtt cgtccttatt tagtgattgc
    11341 aaaagaagat gaggaattag ctgacatgat cagcgggtta gtcaaaaagc aattttttta
    11401 tatcaatatc gatccatatg cgaatgcctt caacgaagaa gctaacggtg ctggccacca
    11461 aacagatcat acaaaaatga acgattggat ctgggaaaga aaatatgaaa ttgattcttt
    11521 atgctaccca atacagttgg cctatcttct ttacaaaaat acaggaaaaa ctgatcaatt
    11581 caatcaatct tttattgaag gtatcaaaaa aatcttggac gtgttcaaaa cagagcaaga
    11641 tcatgaacac tctccttata catttgtacg agacacgaca agaaaagaag atacgctgat
    11701 caatgacggg cgaggtgcaa aaacagcttt tacaggaatg acatggtctg gtttccgtcc
    11761 tagcgatgat acttgccgtt atggatatct ggtaccatcc aatatgtttg cggtggtcgt
    11821 gttaggttat gtggaagaaa tttttcaaac gatactcgat gataaagaaa ttgtacagca
    11881 aacacagaaa ttgaagaaag agatccaaga ggggattcag aatcacggac gaacggttaa
    11941 tcaaaaaggg caaacgattt atgcatatga agtagatgga atgggaaacg ccacagtcat
    12001 ggatgacagt aatgtgccca atctgatcgc cgcaccttac ttgggatatt gttctgaaca
    12061 agaggaagat tatctgcaaa caagaagaac attgttgagt aaagaaaatc catatttcta
    12121 tgaaggaaag tacgcaaaag gaattggatc ctcccatacg ccagaaaaat acgtttggcc
    12181 gattgcatta gctatggaag gaatgacaac gtcagacaaa tctgaaaaag agcggatatt
    12241 gaatttactt gtagcaactg acgcaggaac acaccttatg cacgaaggat tcgatgtgaa
    12301 caatcctgaa aattatacga gagaatggtt ttcttgggca aatatgatgt tttgcgaatt
    12361 agtcatggat tactttgata ttagagtaga aaagtaggag gtatcgtaaa tgaaaaagaa
    12421 agtatatatc atttctcata gccactggga tcgtgaatgg tacatggctt acgaacagca
    12481 tcacatgcga ctgattgaat tgatagacga tctactagag ctatttgaag ttgatccgtc
    12541 cttcaacagt tttcatttgg atgggcaaac gattatttta gatgactatc ttcaagtaag
    12601 gccagaaaaa agacaggcaa tccaacaagc aatcaatgaa ggaaaattga gaattggacc
    12661 attttatatc ttgcaagatg attttcttat cagctcggaa tcgaacgtga gaaatatgct
    12721 gattggcatg gaagaaagcc gtaaatgggg aacccctgtc atgttaggct attttcccga
    12781 tacatttgga aatatgggac agacgcctca attaatgaaa caagctggta tttcagcagc
    12841 agcgtttggc cgtggtgtaa aaccaattgg ctttgataat caagttttgg aagcagaaaa
    12901 ctattcttcc caatactcag agatgtggtg gaaaggacct gatcaaacag caatctttgg
    12961 tctgctattt gccaattggt atagcaacgg gaatgagatt ccagtagaaa aagaagcagc
    13021 tcttgccttt tggaagcaaa aattagcgga tgcagaacaa tatgcctcta cgaaccactt
    13081 attgatgatg aatggtgttg atcatcaacc ggtacaaaaa gatatatcaa aagcaatcca
    13141 tctcgcaaat gaactgtttc cagattacga gtttattcat tctaatttta ctgactatct
    13201 ggaagcagtt caaaaagatg tgccagaaga cttaggctca gtagaaggag aattgacaag
    13261 tcaagaaacg gatggctggt atactttagc caatacagca tctgctcggg tttacttaaa
    13321 acaatggaat accaaggtcc agcgacagct agaaaatatt acagaaccac ttgcgacgat
    13381 ggcttatgaa gttagtggaa actatcccca tgatcaatta gattatgctt ggaagacatt
    13441 aatgcaaaat catcctcacg acagtatttg cggttgttca gtcgattctg tccatcggga
    13501 aatgatacct cgttttgaaa aagcagatga agttggaaag tatcttgcac aagattcctt
    13561 agaacagctg accgcagcaa tcgatacgac cggattccca aaagacagct ttccatttgt
    13621 cattgtaaat actgcaggaa tggataaaac tggtgaagca gaaataacca tcgaattaga
    13681 aagaaaaaga ttcgctgaag gaatacctga acaactatat caagagttag aaaatttgcc
    13741 taaacgtaag tatcatgtag aaacaaaaag tggggccact ataccagcta ttttatctga
    13801 agaaactgtc caatttggtt atgatttgcc gaaagaccgt ttccgtgtgc catatatggc
    13861 aagaatgatc aaagtgactt taccattaga aaacatgcca gcattttctt gggaaacatt
    13921 cgcgcttgta gaaggggaag cgaaagcaga agaaaaggaa acaatgattc atcagagtgg
    13981 acgaatcatc gaaaacggcc ctctacattt gacgattgaa aaaaatggta ccattacaat
    14041 ggaagatcgc aaaaacaaac gaaagttgaa taatctgcat atttttgaag atatcggcga
    14101 tataggaaat gagtatatct ttaaacaacc tttctgtgat cagccaatcc tatccagtaa
    14161 caaagaaaac tctgaagtaa aagtattagt agatacaccg gaaatagcaa aaataagtat
    14221 tctacaggaa atggaaatcc ctgtttctgc agacgagcgt ttagaaaaag aacagcagat
    14281 ggttgtggaa tttcgttatc gaaaagcaga acgatccaaa gaaaaacgaa tccttcaaat
    14341 aaaaacaatc atgacaatca gaaaagatag taaaaaaatc gactttgaaa cgactctcga
    14401 caatcagatg aaagatcatc gcttacgtgt cttgtttcct acaaagttac atgtggaaca
    14461 tcacgaagca gatagtattt ttgaagtggt gaagcgccca aaccatgtgt ctaaaagttg
    14521 ggaaaacccc acgaatccgc aacatcagca agcatttgtc aatattcatg atgaagagta
    14581 cggtgtaaca gtcggaaact ttggtttgaa tgaatatgaa gtaacagaag acggacaaat
    14641 cgcagtgacg ctacttcgca gtgtgggaga actaggcgat tggggatatt ttccaacacc
    14701 agaagcacag tgtctaggtg agcatcggtt caattacagc attgaactac atggaccaga
    14761 agaaaaattc tctacttatt tgcatgcata cgcagcacag attccttttt ccactcaaca
    14821 aatcaaacat catgaaggaa cattgatttc aaaacaacaa tatttaacga taaaaagtga
    14881 aacgtttgca attacagctt tgaaacgcag caagtttagt gataaagtag tagtaagagg
    14941 tttcaatatg tctagtcatt tagaaaaatt ggaaataact aaagacaatg gaaaaactgt
    15001 catattgaat ctgcttgaag aaccaacgaa gcaagcggtt gtcccaataa tccaacctta
    15061 tgagattcgg acaatcggat tcgaggagga aaacgaatgt atggcggaat agaagcaggc
    15121 ggaacaaaat ttgtgtgtgc ggtttcagat ggtggagaaa ttttaga
//
LOCUS       DAZKMK010000067        14673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000067 DAZKMK010000000
VERSION     DAZKMK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.068"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(369..1487)
                     /locus_tag="ACG8V1_001969"
     CDS             complement(369..1487)
                     /locus_tag="ACG8V1_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330058.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1720..1842
                     /locus_tag="ACG8V1_001970"
     CDS             1720..1842
                     /locus_tag="ACG8V1_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330059.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1958..3265
                     /locus_tag="ACG8V1_001971"
     CDS             1958..3265
                     /locus_tag="ACG8V1_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0330060.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3384..4583
                     /locus_tag="ACG8V1_001972"
     CDS             3384..4583
                     /locus_tag="ACG8V1_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0330061.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4610..5878)
                     /locus_tag="ACG8V1_001973"
     CDS             complement(4610..5878)
                     /locus_tag="ACG8V1_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0330062.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5933..5999)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6041..6687)
                     /locus_tag="ACG8V1_001974"
                     /pseudo
     CDS             complement(6041..6687)
                     /locus_tag="ACG8V1_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7095..7598)
                     /locus_tag="ACG8V1_001975"
     CDS             complement(7095..7598)
                     /locus_tag="ACG8V1_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0330063.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7721..8485)
                     /locus_tag="ACG8V1_001976"
     CDS             complement(7721..8485)
                     /locus_tag="ACG8V1_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0330064.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8593..8868
                     /locus_tag="ACG8V1_001977"
     CDS             8593..8868
                     /locus_tag="ACG8V1_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0330065.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9306..10256
                     /gene="yidC"
                     /locus_tag="ACG8V1_001978"
     CDS             9306..10256
                     /gene="yidC"
                     /locus_tag="ACG8V1_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0330066.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10435..10614)
                     /locus_tag="ACG8V1_001979"
                     /pseudo
     CDS             complement(10435..10614)
                     /locus_tag="ACG8V1_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11440..12399
                     /locus_tag="ACG8V1_001980"
     CDS             11440..12399
                     /locus_tag="ACG8V1_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0330067.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSIDFRE
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(13265..13690)
                     /locus_tag="ACG8V1_001981"
     CDS             complement(13265..13690)
                     /locus_tag="ACG8V1_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0330068.1"
                     /translation="MHRKVRITRKSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISI
                     EGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDT
                     LFMDTDSHTIFELNTHRILNKNNKIVIGKHCWIGAKSVI"
     gene            complement(13792..14409)
                     /locus_tag="ACG8V1_001982"
     CDS             complement(13792..14409)
                     /locus_tag="ACG8V1_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0330069.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT         4528 a         3014 c         2431 g         4700 t
ORIGIN      
        1 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
       61 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
      121 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
      181 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
      241 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggaaaa
      301 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
      361 aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga
      421 tccaaaataa acactttccc gccattaatg atcacatcat ctgctaatgc attcagcact
      481 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
      541 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
      601 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
      661 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
      721 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
      781 ttttctggta atgcaaataa ataaatcggc cattgttcag gattttcgaa agaatccttc
      841 aagtacgtat caacagcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
      901 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
      961 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
     1021 tctggtagag atacttcttt tacttgtttg ttgctgacct catacaattt catggagtcg
     1081 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
     1141 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
     1201 taggtctggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
     1261 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat
     1321 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
     1381 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
     1441 ccttctgaag tcaattgtgt caataattct ttatcctttt ttgccataaa cgtacccctc
     1501 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
     1561 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
     1621 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
     1681 gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
     1741 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
     1801 acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaacttccat aacgcgcgtt
     1861 cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa
     1921 tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct
     1981 agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt
     2041 gcaagctgaa gatacgttca aagtaaatgc aaaagcagct tttgctgtag atgcgcaaag
     2101 cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa
     2161 aatcatcggt ctttacatcg tccttgatca agtaaaagaa ggaaaactgt catggggtga
     2221 taaagtttcg atttcagatt atgcagaaaa tttgagtatc accccagatt tatcgaatgt
     2281 tcctttgcat aaagaaaaca cctacactgt caaagaatta ttcgactctg caatcatcca
     2341 gtcagccaat gcttcaatgg tggccttggc agaaaaaatc tctggcagcg aagctaaatt
     2401 cactgaacga atgaaagaac aattgaaaga ttggggaatc aaagatgcaa caatcgttaa
     2461 tgcatcagga ttgaacaact cttatcttgg cgaaaaccga ccagaaggca ctggagaaaa
     2521 cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt gcccgccatt tgatcttgga
     2581 tttcccagaa atactggatg tatcaagtac tacgacccaa atgtttggcg aaaatacgca
     2641 atcaccagtg gaaatggtca actggaactg gatgctccca ggattcgtta attacaagga
     2701 aggcgtggac ggattaaaaa ctggtacgac tgagcttgca ggtgcttgtt tcgttggtac
     2761 gatcactaaa aacgatcaac ggatcatcac tgtcgtattg aatgcggacg gccatgcgga
     2821 aaacccgagt gcccgcttca atgaaacaga ccgtttgatg gactactgct atgacaattg
     2881 gtcagaaaaa gagctcggaa aagctaacgc aagtattcct agtctaaaaa ccattgatgt
     2941 aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa agccctgtaa aagtatgggt
     3001 acgaaacgat atggatactg gcaaattaac gatcacgcca acacttgaca agaagcaagt
     3061 cactgacaat gagatccaag cacctatgga taaaggtaca aaaatcggta ccgctacgat
     3121 caccttggca gacgataaat taggctattt agaagacaac aatgcaccat caacagagat
     3181 cattacggat aaaacagtgg aaaaagcgaa tatattcgtg attggctggc gacgcgtcgc
     3241 agaatttttc ggcaacttgt tttaattgaa caataagaaa aacatctttc catcggaaag
     3301 atgttttttt gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg
     3361 tataagaaaa aggagttgaa tttatgaatc taaccagaga tctgatcttt ttagttctct
     3421 atcttattgg ggtattctct atcgtttatt ggcgaaaaca gaagaaggat ccttccttgt
     3481 tcttttacgg cagtacgtgg acatttacgg taatcttaac ttattttgtc gtgttggaaa
     3541 ttttttattc gaccttttat tttttgattc cattgttgtt ctttagtatc tttgcttttt
     3601 cttatttcac agaaaaacga aggctactga atggtctttt attcaatgtt ttactcatca
     3661 gtttcggcat ctacttgttt gtcttgctgt atgaaacgca aaatattttt cttggcggac
     3721 tgatcgcctt gatcacgatt ccattgctac ttgtcctttt atttggtatt tatggattga
     3781 ttgtttttct tttttggaac ggggtcacag ttttacgtag agaatcgcat tcattagcta
     3841 atcttttaac gttgatacta gccatttttc ttacactgtt tcttgttttt gactttttct
     3901 tgttaaaata tttgccgcaa tggataaatg ctttgttttt ctgcgttccc ctgatcttga
     3961 tctatctatt tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc
     4021 ggcctcgata caatcaagac ttcatcgttg tgttaggcgc tggattgatc aatggagaaa
     4081 cagtttctcc tcttctagcc aagcgaatca ataaggcaat tgctttttat cgggctcaaa
     4141 gccgtgctac attaaatccg cccatcttat tgatgtctgg cgggcaagga gcagacgaaa
     4201 aagtaccaga ggccattgcg atgaaacaat atgccatgga acaaggaatc cctgaaagag
     4261 acattcttgt cgaaaccaat tcgacaacca ctttggaaaa catgctctat tcaaaggaaa
     4321 tcatggatca gcaaatgaaa gggccatacc gcgcgatctt tagttccaac aattaccaca
     4381 tttttagagc cggtctttat gcgcggcaag ccaaattgaa agcaaatggt attggtgcaa
     4441 aaacagcttt ctactacttg cctaatgcgt ttttaagaga atacctcgcc atcttgatga
     4501 tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggctcc gcattcttat
     4561 cgctgattac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt
     4621 ttttttcttt cattctctta actattggca aaaagagtat cccttttttg accgctactt
     4681 gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag cagatcataa
     4741 agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag caggcaatcg
     4801 gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga aaaggaacag
     4861 aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca aacaatggtg
     4921 tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat aacttcccaa
     4981 taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga agactcaatc
     5041 cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct ccacctatcg
     5101 tatttcgaca aacaggatga ggaatccatt gtgcatatcg cgcttttaaa aagacgatcg
     5161 aacggctgaa tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga
     5221 acagcaacat actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat
     5281 agctttctgt catgaaattt gcaaacagtg cagcaaagaa gctagggaag atcgcttctg
     5341 ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc
     5401 caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc cgttctgatt
     5461 tatccaatcg gaacaaacgt gccgtattat ctgctaacgc tccccccatt tggactgctg
     5521 ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca aacagtgtaa
     5581 gtggaataag ccgcaacggg attttttctt ctccaccatt tccttgatcg atcactaagt
     5641 tgtttccacg actagaaagt ccgccgtgat acgcatagag ataagcaaaa agcgcaccac
     5701 taactggtag tagacaaagg agccaaggat gtgatagtcg gatatctgtc acaactgtca
     5761 ggctatttaa gaaaaaagca gacaagctcc ccatgaatat accgagaatc gctgtgatca
     5821 ccagccactt cagcatatac actaaaatgc gtactggact cggtaattgt ttttccataa
     5881 aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc agactttagt
     5941 aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc tattcacttc
     6001 cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct ttctttacac
     6061 gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat tggtcactat
     6121 catgacaaat ataattcccc tttttcgtat acgtgccatc agaacctgct ttcgttgtag
     6181 aaagaaaaag cgaaacattc gatgttttct gacagattgc acagattccc ttatttatcg
     6241 ttggtgataa agtaccgcta atccctttta atttttcatc ttcgtaatag accatgaatt
     6301 ttttctgcgt acctggatca ttccagccga tgtacgtatg ttcttgcaag tcgatttcat
     6361 tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc
     6421 taggctgcat aaacggctga acgatttgct tcagctcctc caatgctctt tctgcttgcg
     6481 cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgattctg gcagttcgtg
     6541 atctaatatg tctcgaatct tttcagctgc tagtgcctga actgtttgta ccgtttttgg
     6601 atcattgaca gatgagtacg catttaataa ctgttctact tctcttatta taaaaaaata
     6661 ttggtatggt tttattgttt gttccatgat tttaaagtcc tttcttctaa ccaaagtgca
     6721 tactttgtag aaaaaagtga tcttctgcaa agtatgtgtt acctgaagat cccggactct
     6781 tttaaataag atacgttttg catcttatca tcaccccctt taatgcttat tattctatgt
     6841 acatgattca ttcgataaat aaaagtaaac cgtatcttga acagatagtc aactgcaaac
     6901 agaaaaaagg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaatg aatggtgttc
     6961 aaaagctggc atctgcggta ataagccgaa acttcacaaa aatttgagaa gcaattttcg
     7021 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaat atggtctatt
     7081 attggtttta tctattaaat cgaggattct ttcccaaagt aacgttgcca tctcttacgc
     7141 atacttgctg acataggctg agcggcttct ttgactgcgc agtatttgtc tacgaatgtt
     7201 acgatatatc cttctttgta cttaggcggt gcaatcgttg cgccccacat gtgcttcaaa
     7261 atgatatctc gctcaagatc cgacaattcg gtgatctttt ctgcattctt tacagcaatt
     7321 cttggatgga tatacgcatg tgtgccttca tcaaatttcg tcgtacgcca atcatagtag
     7381 aacagatcat gcaaaagtcc agcacgcgct gttgctttag cattgccacc ccattttttt
     7441 gcgagcagat aactatagta agaaacactg atggaatgct ccaaacgagt ggaatgaaca
     7501 tgctgtgtgt aattcgctaa ttttttcact gcttctgttt ctaaaagatc ttctacataa
     7561 gacatatatt cttcgtcttc acgccatttt tctgtcataa ttatcgatct gccacctttt
     7621 tgaatttcat tttcttctga aattgatcta agtataccac cagtgttcgg attattctag
     7681 tcgccttgga taaaatagcc aaagcttaat agttccttaa tcataaagaa aaatgataaa
     7741 gaacaatccc tgccgcaacc ccaacattca acgattccgc atttcctttc atcgggatat
     7801 atacgttctg atcagtcaaa gacaagattt cctgacttac cccctgacct tcgttcccca
     7861 tgattattgc gaagttatct gatttttcta ctttgtctaa tgcgtgtgct tcttcattta
     7921 attctgtgcc atacactgta aaccctttgt ctttcattaa agaaatcact tctgtcaaat
     7981 ctttttgcaa cacaggcaaa tgataattgc ttccctgcat gctgcgcagc acctttgtac
     8041 tataaatatc tgccgttcct ttgcctaata aaacaccagc catatcaaat gcatctgccg
     8101 tacggatcat cgtccctaca tttccaggat cttgtacatt atctaacaac agccatcctc
     8161 ctgaaaaatc tggcgtgcct tcagccgtta acgccatcac tgctaagatt ccttgtggtg
     8221 taggtagatc cgatacagca tccatgacct catccgaaac tagaaaagta gaaatctctt
     8281 tgtttgcggc cagccagtct ccccattctt tcatgcctcg ctgtgtcaca aaaagttctt
     8341 ttatttctgc atcagacgac gcagcttctt caatcagatg gaacccttcc aaaagatatt
     8401 cttccttttc ttcccgatat ttctttttat gtagtttttt taattctttg attcttggat
     8461 tttttgctga ttggatctct ttcacattcc tcacctcccc atgattatat catggaatag
     8521 gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt aatttataaa ataggaaaga
     8581 aaggtgacga acatgcgcaa agttaaaatg aacgtccaag gacgcgtcca aggcgtagga
     8641 tttcgctaca tgaccaagat ggtcgctgat caactaggcg tgactggaac ggtaaaaaac
     8701 gaagatgacg gctcagtcac gatcgaagca atcggcaatg acgacatcat ccagaaattt
     8761 attgaagaag tcaagaaatc tccgagccca agtggacgag tgcagtatgt ggatgtccaa
     8821 gaagacccga tgattgagga acgaaagaaa tttgatgtgg tgggataagg ttgtgaaaaa
     8881 gctggtctgc atcaagcatt aaggacaggt tgtgaaaaat agctctccat atttttcaac
     8941 aggctggact taatgccgta gatgcaagct tttgaacacc gtcgataagg ttattcttga
     9001 ggcgctttgc ctcaaaaatt aaggacagca gtgaaaaagc tgatctatca cttgcagtga
     9061 gttcatttga ctccgctttt tcgggcagaa aaaaagtttt tagtttatta aagtaagaaa
     9121 gaaacacttg gaatcattct actttttcct agtatttctt tcttgatttt ttgtcttttt
     9181 ttcaaaattt ttcgctacgt aaaagaacga atatttatat gaaatttcta tgaagttgat
     9241 tgaattccat gcgctttttt agtatagtta agtttgtgta aaaattatag aagaggaagt
     9301 attctatgaa taaatggaaa aactggttat tgggatccgg acttgtcagt atcctgctct
     9361 ttttatctgg ctgtgtgcgg atggattcaa acggaaaccc tgacacttcg ggaatcgttt
     9421 atcgagtact cgtccatcct atgggccaag cgattacata tctagttgaa aatttcaatt
     9481 ggtcttatgg ctgggcagtt attgcaatga ctgtgatcgt ccgtatcatc atcttgccac
     9541 taggtattag tcaatcgaaa aaaacaatga tccaatctga aaaaatgcaa gcgttaaaac
     9601 cacaagttga agcagcccaa caaaaattga aagctgcaac aacacgtgaa gaacaaatgg
     9661 cagcacaagc agaaatgcag caagtatacc gcgaaaacgg attgagtatg accggcggaa
     9721 tcggctgtct gcctctattg atccaaatgc cgatcttctc tgccttgtac tttacggcac
     9781 gttatacaga aggtatccgg gaatcatctt tctacggcat cgatttaggc tcaccaagta
     9841 tggtacttgt tgccattgca ggggtcgctt atctgcttca aggatacatc tcaacgatcg
     9901 gtattcctga agaacagaaa aagacgatgc gtacgatgct gatcgtctct cctgctatga
     9961 tcgtcttcat gtcgatcagt gcgccagccg gtgttacact ttactgggta gtcggtggtg
    10021 tgttcagctg tctgcaaaca ttcatcacaa acgttatgat gaaaccacgt ttgaaagcac
    10081 aagtggcaga agagttgaaa caaaacccac caaaacaagt cgtcactcca agaattgatg
    10141 tgacagaatc agctaaacca gctgacagca aacaagcaac caaacaaatc aagcaaccca
    10201 aaaacagtac aaacaataaa ggccgcaacg caggaaagca gcagaagcgg aagtagatag
    10261 taaataagag tgtaataaaa attcattaac agttttagca attaaaaata tcgaccaaga
    10321 tttttgaaaa agaaatctgg tcgatatttt tatttaagtg aatgtttaat ttgaatcatc
    10381 gattgatcgc tatctaattg cagcagcatc tttttatgaa gaaacaactt tttcttatct
    10441 atcaacgtag acaggtgttg ctgtttcttc taatagtgta cttaattcct taatattttg
    10501 ttttccttct tctaacatcc aactactcgc tttttcttcg ccttcggtaa caaaaggcgc
    10561 cactcccttc gcccatgttg ctcgcccaca taagacacca ttctccataa aggacaacgc
    10621 ggagatacca cccatctaaa taacatccat aaacctttct agtagaagaa tagtgatagt
    10681 atccatccct ttttctactg ggaatacaat tacacgattc aaagcttgat acacaacatt
    10741 tcccttctta taaaccgcta ttgtaaccga attgttttga atcacctttt tgggatagaa
    10801 actggctcct ttccgcaact tcttaaatgt tggtttcttt tatactttct ttgtagcaac
    10861 tacaaatttc ttcaaatctc taaatgtttg cataacaacc gcctttctga cttttactca
    10921 ggatggtatt taaaaatcag caaaataagg cttctctcca tcttttttat ttcctagctg
    10981 ctttaaagac actcttgctg ttttttaatc tctatcaagg gggctctgaa tctattttga
    11041 tgaacttatc ttattaattt ctcaaatgag ctgtcagaca caactgtatt ttttcaatct
    11101 cataccggac aactatgcca gcttaaaaaa atttcctctg gcaaaacttc tttattgaaa
    11161 aatgacaatg aaaaaagacc attctttttg ttgaatgctc attgtgtaaa catatttcct
    11221 gcgactgctc ttcgaaccat tctcagtgca atccctaatt tatctattaa atcttctttt
    11281 agttcctcat ctttcatatt caaaatttcc tttgtatgtt acacaagttt gttctctaaa
    11341 taacgcgaat tgtaaaatta agttagaaaa ataaaaaggc atttatggta cactcaaatt
    11401 gtatttccga cgaaagaaaa caaaggagtg taaccataaa tgacctacaa acatcttacc
    11461 atagacgaac tgacaatgat agaatcatat tatcttcaac ataataagcc ggttgaaatc
    11521 gctaaccgaa tgggacgtgc tatacaaact atttataatg tagtcaataa gttcaagcaa
    11581 ggcaagactg ctcttgatta ttggcaccag tataaagaaa ataagaaaaa atgtggtaga
    11641 aaagtcattc aattacctgc tcatgaagta gattacatta aagagaaagt cactcttggt
    11701 tggacgcctg acgtcattat cgggcgaaaa gaaaggcctg tttcatgcgg tatgagaaca
    11761 ctttatcgtt tattttctaa aggaatattt gatattgaca cactaccgat gaaaggtaaa
    11821 agaaaaccca atggccatca ggaaaaacgg ggaaaacaac agtatcagcg ctcaatccat
    11881 gatcgacctg ataattatcc tgatttcaat tctgagtttg gtcaccttga aggtgatacg
    11941 atcgttggca ttcatcataa aagtgccgtc attactttag ttgaaagatt atctaaagtc
    12001 attatcacga ttaaacccaa cggccgtaag gcattagata ttgaaactgc ccttaatcaa
    12061 tggttttctc gcttccctaa aaacttcttt aaatctatta cgtttgactg tggaaaagaa
    12121 ttttctaact ggaaagccat tagtaaccaa catgatattg atatatattt tgcggaccct
    12181 ggaacacctt ctcaacgccc attaaacgaa aattctaacg ggattctgcg tcgtaatgga
    12241 ctgccgaaat caatagattt tagagaagtg aatcagacat ttatttccag tgtcagcaat
    12301 caacgcaatc atattccaag aaaatcattg aattacagaa caccaatcga aatatttttg
    12361 agctatgtac aagaagcatt ttattctagc ttaatttgac aaatcatata atttaaaatt
    12421 aacattgatt ctcctatgaa aatatctttt acctttctcc tgtgaaaagg aaatttcctt
    12481 ttttatagtg ttattgatat aatcagaata ttcatcaaac tctgtatctg gagttaaaaa
    12541 tctacagtaa catctataag ctttgctagt aatcttcttg ggttctctct ccattgtcaa
    12601 gcaacatcaa aacacctact tactattcga ttcttttctg ccaaaaacga gtcagcatca
    12661 ctcacaactt atgtgttgct gactccgact agtcctttgt tcaatatcaa cttattaaac
    12721 agatataata tttttcttta tttcgtatac tccaagactt ccatctttct ttcatcttat
    12781 ctctgaaaaa aacacagcaa agtgccctta ataaatatac attagaattt gcaaattttt
    12841 tcatcttaag atatatccta ttcatattct ttacaatcct aatgggtgat tacaaatcta
    12901 tttagttcaa gttctacatt ataaacacct ttcttgatag tatcatctgc tgcacaagcg
    12961 atgtcgatcc acttattagg ggatttacgt gctcaaaagt ggctattcct tttgggaagt
    13021 ccatccataa taatggtcca tggatttttt tagggtcttg tcgttcaaat ttctcaaaaa
    13081 ctttattata cttccatagt tttataatta tttggtttca ctttggctcg ttcaaacacc
    13141 aacttgtatt tcttttcaac gttcgaactg gagccccccc tataatctca ttattatttt
    13201 catgactttt tactaccgtg gaattacaag caacaacaat attatctgct aaatgaacac
    13261 ctttctatat cacgcttttt gctccaatcc aacaatgttt acctataact attttattat
    13321 ttttattcaa aatcctatga gtattcaatt caaaaatcgt atgtgaatcg gtatccataa
    13381 ataatgtatc ccacgatact gtacagccat ctttgaattg aatttcctta aagcaaatta
    13441 tactcatttt tcctgtcgaa ttaaactttg acccaatatc taaaactgca tttttcctta
    13501 tgtcaatatg agccccttgt gcgataaacg ctggcccttg tattcttagt attccttcaa
    13561 tacttataat cgtttcttca tttcccgcag tatttaatac tggaaagccc aaataaacta
    13621 tttttccaga ttgtaatact atctcaattt tccctctcga tttgcgtgta atccgtactt
    13681 tacgatgcac caaaataggt aatctccacg catctgaaat atgaagaaat ttataattga
    13741 agtatatcgt tttaggtaaa tttaatatat tctttattat caatccgagt ctcatctttc
    13801 accatctctt tttgtaatca aaattagttt ataaaggatt acacccaata taccgcccaa
    13861 cgtattatat acaaaatccg aaatttgaaa cgttcctagc ctcaaaaaaa gttgtaaaaa
    13921 ttcaattatt gctgaaaaat aaaatgcaat ttttcctcca tatactattg aggaccatat
    13981 tttgggtttc tttatatttt catatttaat agacattacc atataaacat atggaataaa
    14041 aagaataata ttttctaaac acgctgtaga taactctttt gttccagttg ttgtattata
    14101 cttataaatc caccagtctc ccataacatc agataaagga tttaaccaaa ttgttcgatt
    14161 gattaatgtt gtaaataaaa tcatcgcagt atagaatatc aaagcacaaa cttttctata
    14221 ttttctatcc ttctttaaat gaagataaag ttccattagt atacgtttat agccctcacc
    14281 tgcagcaatg ccatctgttg catacataaa atagaccagt cctaaaattg tcaataaaac
    14341 agcaaaccca aagggttcat ataatttata taatatatct gtaataattt ctgcaatcac
    14401 tattatcata attatttctc caatatcaag tatacactta tcgcttccaa ttacacatga
    14461 tcatattaga gtgtattatc acattctcta atattcaatt tatttttcct aaaatattcg
    14521 aaataatcgt cttgataacg agtaagtatt tttatacgat tgataacata gtaacaatat
    14581 ttttaattat agtaaaaaat catctgcctt tttcatccac ctaatcttat ttttcatttt
    14641 attcaacatc cttaaataaa cttccgttcc taa
//
LOCUS       DAZKMK010000068        14625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000068 DAZKMK010000000
VERSION     DAZKMK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.069"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8V1_001983"
     CDS             4..816
                     /locus_tag="ACG8V1_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0330070.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8V1_001984"
     CDS             813..1613
                     /locus_tag="ACG8V1_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0330071.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8V1_001985"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8V1_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0330072.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8V1_001986"
     CDS             3110..4819
                     /locus_tag="ACG8V1_001986"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0330073.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG8V1_001987"
     CDS             5031..9389
                     /locus_tag="ACG8V1_001987"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0330074.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8V1_001988"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8V1_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0330075.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8V1_001989"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8V1_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0330076.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG8V1_001990"
     CDS             11227..11520
                     /locus_tag="ACG8V1_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0330077.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG8V1_001991"
     CDS             11517..11828
                     /locus_tag="ACG8V1_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0330078.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG8V1_001992"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG8V1_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0330079.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8V1_001993"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8V1_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0330080.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DAZKMK010000069        14621 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000069 DAZKMK010000000
VERSION     DAZKMK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.070"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..436
                     /locus_tag="ACG8V1_001994"
     CDS             <1..436
                     /locus_tag="ACG8V1_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0330081.1"
                     /translation="IKIDLPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALE
                     FLIKDYAISKNSEDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRH
                     TNKDVHDMDAFIKSAAQFIAADYDADLADQFINGNNFMPTTN"
     gene            complement(582..1058)
                     /locus_tag="ACG8V1_001995"
     CDS             complement(582..1058)
                     /locus_tag="ACG8V1_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0330082.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(1018..1374)
                     /locus_tag="ACG8V1_001996"
     CDS             complement(1018..1374)
                     /locus_tag="ACG8V1_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330083.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(1352..1537)
                     /locus_tag="ACG8V1_001997"
     CDS             complement(1352..1537)
                     /locus_tag="ACG8V1_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330084.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1538..1810)
                     /locus_tag="ACG8V1_001998"
     CDS             complement(1538..1810)
                     /locus_tag="ACG8V1_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0330085.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1807..2007)
                     /locus_tag="ACG8V1_001999"
     CDS             complement(1807..2007)
                     /locus_tag="ACG8V1_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330086.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            2135..2347
                     /locus_tag="ACG8V1_002000"
     CDS             2135..2347
                     /locus_tag="ACG8V1_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330087.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(2380..2574)
                     /locus_tag="ACG8V1_002001"
     CDS             complement(2380..2574)
                     /locus_tag="ACG8V1_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330088.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(2587..2769)
                     /locus_tag="ACG8V1_002002"
     CDS             complement(2587..2769)
                     /locus_tag="ACG8V1_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330089.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(2766..2906)
                     /locus_tag="ACG8V1_002003"
     CDS             complement(2766..2906)
                     /locus_tag="ACG8V1_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330090.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(3396..3761)
                     /locus_tag="ACG8V1_002004"
     CDS             complement(3396..3761)
                     /locus_tag="ACG8V1_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330091.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(3801..4103)
                     /locus_tag="ACG8V1_002005"
     CDS             complement(3801..4103)
                     /locus_tag="ACG8V1_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0330092.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(4103..4402)
                     /locus_tag="ACG8V1_002006"
     CDS             complement(4103..4402)
                     /locus_tag="ACG8V1_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330093.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(4395..4559)
                     /locus_tag="ACG8V1_002007"
     CDS             complement(4395..4559)
                     /locus_tag="ACG8V1_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330094.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(4565..4834)
                     /locus_tag="ACG8V1_002008"
     CDS             complement(4565..4834)
                     /locus_tag="ACG8V1_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330095.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(4846..5595)
                     /locus_tag="ACG8V1_002009"
     CDS             complement(4846..5595)
                     /locus_tag="ACG8V1_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330096.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(5592..6284)
                     /locus_tag="ACG8V1_002010"
     CDS             complement(5592..6284)
                     /locus_tag="ACG8V1_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0330097.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(6290..6961)
                     /locus_tag="ACG8V1_002011"
     CDS             complement(6290..6961)
                     /locus_tag="ACG8V1_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0330098.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(6954..7295)
                     /locus_tag="ACG8V1_002012"
     CDS             complement(6954..7295)
                     /locus_tag="ACG8V1_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330099.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(7473..8171)
                     /locus_tag="ACG8V1_002013"
     CDS             complement(7473..8171)
                     /locus_tag="ACG8V1_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0330100.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            8258..8728
                     /locus_tag="ACG8V1_002014"
     CDS             8258..8728
                     /locus_tag="ACG8V1_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330101.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(8771..8950)
                     /locus_tag="ACG8V1_002015"
     CDS             complement(8771..8950)
                     /locus_tag="ACG8V1_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330102.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(8937..9275)
                     /locus_tag="ACG8V1_002016"
     CDS             complement(8937..9275)
                     /locus_tag="ACG8V1_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0330103.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(9291..9494)
                     /locus_tag="ACG8V1_002017"
     CDS             complement(9291..9494)
                     /locus_tag="ACG8V1_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330104.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(9509..10285)
                     /locus_tag="ACG8V1_002018"
     CDS             complement(9509..10285)
                     /locus_tag="ACG8V1_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0330105.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            10584..10904
                     /locus_tag="ACG8V1_002019"
     CDS             10584..10904
                     /locus_tag="ACG8V1_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0330106.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            10922..11353
                     /locus_tag="ACG8V1_002020"
     CDS             10922..11353
                     /locus_tag="ACG8V1_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0330107.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            11483..11944
                     /locus_tag="ACG8V1_002021"
     CDS             11483..11944
                     /locus_tag="ACG8V1_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0330108.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            11978..12613
                     /locus_tag="ACG8V1_002022"
     CDS             11978..12613
                     /locus_tag="ACG8V1_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0330109.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            12726..13361
                     /locus_tag="ACG8V1_002023"
     CDS             12726..13361
                     /locus_tag="ACG8V1_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330110.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13354..13488)
                     /locus_tag="ACG8V1_002024"
     CDS             complement(13354..13488)
                     /locus_tag="ACG8V1_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330111.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            13660..>14621
                     /locus_tag="ACG8V1_002025"
     CDS             13660..>14621
                     /locus_tag="ACG8V1_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0330112.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT         4550 a         2705 c         2273 g         5093 t
ORIGIN      
        1 gattaaaata gatttaccag aaaatattga aaaagtttct cctacatttg ttgatattta
       61 tactcaagcc acacaagcta aatctgagca attggaacaa attgctggag ttggttatag
      121 aaaagctcta gaatttctaa ttaaagatta tgcaatatct aaaaattcag aggatgaaga
      181 taaaataaaa aaaatgttat taggtaaaac aattgaaaat tatttgaatg attttcctaa
      241 actccaaaga ttagccaaag ctgctacttg gattggtaat gatgaaacgc actatgttcg
      301 tagacatact aataaagatg ttcatgatat ggatgcattt attaaatcag cagcgcaatt
      361 tatcgcggct gattatgatg ctgatttagc tgatcaattt ataaatggaa ataactttat
      421 gccaactact aattaaaaga ataaaagagc cacagatacc aattaataaa acggttggga
      481 tatcttggct ctttcatatt tttttgacac taatagaata tcatgctgct aacaatgttt
      541 acatagctac aatgaccgaa catttagtga acagtgtaat tttagaataa agagagttgt
      601 tctttgtact tctcgctatt gtaaaaatca atttcgtttt cttttttcaa tcggtcagct
      661 tgtctgtgtt catactcatc taaaaagttc aatgtctttc taatttctgc atgtcgctgt
      721 ctgacatacg atgagctata cccaacagat tcagcaatat cttctaagct catttgatca
      781 acatatttca tcttaacaat ttgattatcg ataccagaaa agctatctat cagcaataac
      841 atttcttctt tttgctcgat caacaattcc aattcatttt ctatcttttg gatattctct
      901 tctaaagaag aagttctcga attcttttcg atgcggacgt ttgctaaatc accacagacc
      961 catcgattca attcaagctt acttttatta agattccact ttaagtaaag aatctgctca
     1021 tctagttctt ggtaatcttt taaccaatga aatctcacaa acgccacccc ttatggtaga
     1081 atagtattgc gctgctatct cgaaaggggt agctttttta ttttttctta gccgttccat
     1141 tctggattgc cttttgttcc aaacgtcgtt ttttcttctt aatctttgat tttgtttttc
     1201 ccatacaatt ctcctttccg aaaacacatt ttgttcgatt tttaatacta aataaaagtt
     1261 ggccagaatt tttcgttcgt ctttactcat ctttttttga ataagattac tagcataaaa
     1321 atagttagta ccacgaaaat gaacaggtaa atcaattacg ttcaccttct tcatgccagt
     1381 tatcagccac tagaatcttt gcgttagtgc cgccaccgtt ttcttttttg tatttttcaa
     1441 atgctttata agcagctgtt tttgatttat agatttttcc ggaaccgact gggaacatcc
     1501 atgtgccctc tgtgatagct aagatgtata ctttcattta attcacccct ttaatcccca
     1561 acactacata tccggacttc tgcatgtaat cagtaatata agtgatctct gctttgtatg
     1621 agtcaccagt gtaactaaat gttggtctcg ttagtccagg taattcatgt ttaatgtatt
     1681 ttttatattc acgtagtatt aactgatcac caactttgaa gtcacgatca ttcttgcgga
     1741 tctcgaattg tttgcgtccg ctcgtcactg cttcaaaata ttctggtagt atttttagtt
     1801 cgtggatcat tctgtttcct ccttcaaaat tcgctcatca tcaaaaagtg aatctccaca
     1861 aaatccttct cccaaatatt tatcgttcat gtataagtca accaaatatt tgtgatctgc
     1921 ttctcgatat tcattaatat taacgatctt tattttgtaa acattacctt cgtcgctcac
     1981 tatcttgaat tgttctctga ctttcaccac cgttcctcct ctttttgaaa aaattttagt
     2041 gtttttgtaa gaccccctaa atcagctttt ttatttgata taatacttgt atcgattaat
     2101 caaatcgaaa ttaaatttaa aaaggtgatt tatcatgaat atcaacaatt tagtaaaact
     2161 cgttgaacaa cgttttaaag aatcaggatt agaacaatta aaaaaagata attctccaga
     2221 attattttct aattttaccg aagacgacat tcctactctt aaacaattaa aatattattc
     2281 tgattattgg acgcaaactc gtgagaatga attaaattta gaaaaactca ttattaaaaa
     2341 gaaatagcaa ctccagccca cacggctggt tttttagttt caacttccct ccttacgggt
     2401 caaaatcatc ggttccggca taatctgatc atcctttagc aacaaatcag gatatttttt
     2461 ccttagcttc ttattgttcg tgtaagtttt caatagccat tggttggctt cgcttttgct
     2521 ttctccagaa aaataaattt tccctgtttg gatgcctgca agttgaatca tcattttcac
     2581 ctcaaatcaa attttttctg ctagatggag caaaagcaat ccgacatcaa gaatcttttg
     2641 acccattatt tcagctactt tgctcggctt aactccttga gaaaagagca agcgtgcttg
     2701 ctctatctct tcgtgagtcc aaataaattt ggcttcctca aggataatta ctggatcagt
     2761 tgctctcatt gttcctctgg aaaaatgatg tcattcttat gcttggacca ggtatctgca
     2821 tatggaacaa agtattgtct agctaattcg atttgatcaa ttaaagcagt ttctgataat
     2881 tcatggtctg cagcaatttc attcatacta tcgccttcat ctaaacagat aagaccgttg
     2941 acgttatcga tttttacatt tgtgactgct tgactttctc cacgctcata atattctaca
     3001 gttgatactg atacatcagc ttcggagttt aattgcagaa tctgttactg ccgggataat
     3061 ttaatattct tttcataatt cacctttttt cctactgaca tcgaatattt ttcatgttat
     3121 gattagccgt gaaaggagag ctatattatg tgtttacaca tgatgtcctt agctcattcg
     3181 gttctttgga agcttttttg gaaataactc tcaggttctt gacgcaaatc tcatttattc
     3241 tttgaaacaa aaaacttcca ttttccgaga gctttggatc acttcaaaag agatttgcat
     3301 ttatatttct gtgaccttga gtcgcgtaaa gaaccttaca gcaatggcgg caattccact
     3361 ttcaggaaac cgcctttttt cattgatatt ggtggttagc ggactaccca ttttctttcg
     3421 gcagtgccaa accacaaatg atctgtgttg atgtttaggt agttgtatac cagataagct
     3481 aaatccaaag tgcctgcatc ttcaagacga caatattcca aaaattctcc catagctgga
     3541 cataaaaatc ctggagctat catatcccaa cagttaatag tgcaaagaaa ttcgtttagc
     3601 ttctgaacgt tttctttggt ttcacgaatt acaacatact ccaattcttc tccgctggca
     3661 gagcattcca aaacttcaaa ttgattaaga aatggtagaa catatgcttc aaacatcaac
     3721 attccatctt tctgtttttg cacttgtttt gcatcaatca tgtctatcct cctattcagt
     3781 tatttcttcc attaatggaa ttatttcagg tcactggact taacgaaaac accattgacc
     3841 atctttccag tccgaccttt gatttcgtca taagcaaaat ccaagcactc gtataaatcc
     3901 atatcatttt gcattgccaa gatgatcaag gttactacta catcgccaat tccgtctctt
     3961 aaaccatgtt catcttttct agctagagaa gcggcaactt ccccgatttc ttcgaccgtt
     4021 tttaacattt gcttgctgaa atccgcttga tccaatccct tatcttttgc ccactgctct
     4081 actttcgcga ttagttcgtc cattatttct cctccacata cctaaactgt cttccttttg
     4141 aatcaatcca taagctccta gctctatccc aaataatatt tttgctcaga ccagtaattt
     4201 cagataactg tttagcggta cccgttacta gaattcgatc accatgccag attgcaatct
     4261 ttctcggcgt tctccgtttg ggcttttcag tccacattga tttaccgagc ttttggactt
     4321 ctgcaactat ttttttatct tcctgccaat cttctgaata agtcaattcg ataattcgct
     4381 gcattgccgc tttcttatcc atcccgacat tctcctttca gtaatttgag tacttgatca
     4441 agtgcgctct cacgtccgcc atgaaatgtg ttgagccact tgtcttcgta cgacacactt
     4501 tgtcttaaag cttcttgatg cattaattcg atctgtgctg taaatgtctt taggtccatc
     4561 ttgtttacac ctgctcaagt tcattaagat gtttttgcaa gcctttaaca caatcaatga
     4621 atagtaattt ggtatatgct aaatttctta attgcgttcc atcgatatag agtgcgaaat
     4681 agtatctgag tttactccaa ctcgaacgat cattcttaat tccttcaatt ccagcttctt
     4741 cgagttgatc atatacgtct ctcaggacgt ctaattcctc gcccttttta taattcgcta
     4801 tttcattaat tagttctaga taatcgatct tcatttttcc acctcttaga atggtgcttt
     4861 tgattgtcta ttagctcgtt ctagcgcttt tttcttgaga taggcttctt ggtcgattgc
     4921 ccattcagga agcttctctc gtcttcctgt gcgcttgtat ccactgcttg cgttcttagg
     4981 ttcacttttt tctttccttg cccaacttcg aatagttgcc aaatagtttt tataagtctt
     5041 accagatgat tcacaatact ctgacagtcg ttcgattcgc tcttggtaat cattagggaa
     5101 ttctgttttg agtttctcca tctgctcatc tgacaaaaga acatttttat actctccgta
     5161 tttatgacgg acgggcttag ccttcgattt tttcgaaggc ggtaactctc ttatatattc
     5221 ttttgtatta ttaaatgtat tattaataga tgtattatta tctttgactt tttcgtcaat
     5281 aggggtattg cgtttttcgt caatagggta ttgattaatt cgtaggtacc tattgattat
     5341 ttgattggta ccctctttgt aaatgatttc ccgattcaag tatccaaact taatcaaatc
     5401 acttacccat cgcgatatgg tctctttatt cacaccatat aaatctgcaa agtactcatt
     5461 gcctgcccaa caaaagcctc tttcattaca caaggccgtt atctctccgt ataacaactt
     5521 agtatttggt ttaagtcttt tgtcgtacct tacgttggct ggtataatcg cataataact
     5581 tcgatgttct gtcatttttc ccctccaata tttaactttt tgattgtttt ctggtttaat
     5641 ttgatccctt tgatttgata tttatttttg aaattaatca cacctatttt gtgcttctct
     5701 gtgtgatgga ttctgcagag tgctgcaaat gtgtactctg catgatcaac ttctttgcgc
     5761 tttcgtcttc ctagcgcttt gtcaaagtga tcgatgtcag ctcctgtttt gccacagatg
     5821 cagcagactc tttttgtgat gcatttgtag aagtaatatt cttgattcgc aggtaaaatc
     5881 tcatagcctt ctttgaaagg aatatgatgt tcaaagatga aatctaagat gatatttgct
     5941 aagacattgg catcactcac agttgtattc gattcgtctt tcaggcttat tttgcgccct
     6001 gtgacacctt caaaacgaaa gtagaagaat tccttccaga agtccgttgg catgcctgta
     6061 tcgataaaaa tatcgcctat cagcgcatag atgaagtttc gttgctgtac ggtgaaacgt
     6121 ctaggatcaa taaaacgaat ttcaatgact cgatcaccat cgtagccgtc atacatcgtc
     6181 tttagtcgat caatgttcac ttcctcattg atggttgcgc ttatgtcttt tcctttgaac
     6241 tttttcagaa ccgctgaata tgaatcgatt aatggtttaa acactcatat cacttcttat
     6301 ctaattcttt tctcttagct gctattgctc gctccatcaa ggcacattgc tcatagctta
     6361 actgttcaat agtttcaacg ttatcagcta agagccctaa tttatctgtc tgctcattaa
     6421 catattcgat taaggttttg gtcatatctt tacccatctg ctcattgaaa gcttctagaa
     6481 tcgtctctag catgtttaat ttctttgtat cgattctagg tggtgttgga atatcttccc
     6541 cttgaaatac atataatccc agtccgtgta gagccaatgc tttcacaaag catcgcttca
     6601 atgagttatt gatttgcatt gcatttggtt taacaactgg ttggtttcga taatctaaaa
     6661 caggaaataa ttcggtttcc gtgtgtcctt taaccgttac tgagacagat acataagtcc
     6721 cagtttcatc cataagaaaa ggtttatatt cctcaacaag aaagtcttga tgagttccag
     6781 aaacaaccct gtagtgtttg tactcattaa tagttaccgt tgcctgtgga tcattctttt
     6841 tcataatctc ccacgcgtga gcccaagata aataatcaaa atttcctttt ttcttgagaa
     6901 ttttatttaa cttacgacta aaaagttttt caaagttcgt tgtccctttg atttcactca
     6961 tcaaattctg cctccatttc agcaatgtat ttcttacctg gtccgtaata agagatatca
     7021 atcaagttat ctctgtcgta ctcttctagc gcatcaatca agccatcttc gatgacgtaa
     7081 atatattcag gtttattcga atgcttcgat agatggataa gataaacatg atcccaaata
     7141 gttacatggt tgcctaaatc atcttgatcc caagctagtt cttcattcgt caaaagattt
     7201 cgtctgattt ttcgaccact tgtttcctca attttcggct tgccccaatc aggatcaatc
     7261 aaatattgat ctagagtgga aagttctttt tccatatgct aaaatctcct tatgatgtgt
     7321 tttctttgtg actctttgct tgccggcgga gtcacttttt tatttgttgc catgcttttt
     7381 gcttgtcaat atgttgttgg cttaggatga ttggtttata gtatttccac cagcaattag
     7441 caattgccgt ccctattctt agcgcttcag ctctattcat tgtcatcacc aaaaagtctc
     7501 tgttgtctgt tcagttgatc aatttccata cggatcgcag tttctggcaa ccacatttca
     7561 ataaatgaaa cagcatcatc gaatctctta cgaggtaact cgccatatct tgggattgaa
     7621 aaggtacgtt taaattcaga ccaaaatttt gagaatactt ttttgctgat ttcttcataa
     7681 gctcggcttt cttttccccc tagaactccc ataactttca tatttccttt ttgcttaatt
     7741 tcaaactctt gttgtccgct aattcgcata gtatctttaa gcatggaaac atctttttta
     7801 acatctttca tttcttctaa ttgatagatc atcatatctt caattgtttg aggaacagta
     7861 ttcttgcgaa taacatcttc catttcgttg aatgcttcaa tgtatttttg tttgaagtag
     7921 atagctttct ttcctgtaaa ccccatagcc agcaagaaaa atccatctct actaatgaag
     7981 aaaactcgtc gatttctgcc atatgaatct ggttcattac cttctacaaa catctgtcca
     8041 aaattggaca catctttttt tagtgcatca atatctctta aaacatgttg atgttttttc
     8101 tcgaagcttt ctgccacttg taagctcgta gtcacagctt ctttattttt caaaattact
     8161 aattcttgca ttatttcttc tctccttttt ggtataattt agttaaaaac tggtggtgtt
     8221 tattttgaat ttttttcact taacaatttt tcaatggatg tcaataataa gttttatatt
     8281 gagtacgttt ttaacaggga taaaaataaa aggaatgcgc cctcaactag aagttgaact
     8341 taatgcttct tacttcgctg cagatatgat ttatacaaaa gtaatcattt ctaactattc
     8401 aacagaacca gctatgttag ttaatttaga actttcatcc gctaacttaa accgcagatg
     8461 gtcagctact ccatttaaga aattaattgc taaaggagga tcagtcgatg ataatagaat
     8521 atactctgaa gctgtaccgc taaatattcc tcctaaaagt gctatatctt gttaccttgc
     8581 atttgaagta gggaaaatta attttaataa actccttaat caccaaacaa aaatgatatt
     8641 tactctaaat agaacccaaa tccacaaaat tgttgatatc aaaaatacaa acttcccaat
     8701 agagaaatta gtgaaagaat taaactgagt aatagtaata tacattctag tttctctttt
     8761 ctattcattt ttagtcagcc ccctcggttg gctttttcgc tctgtactca gcttcagctt
     8821 catcaagccc cataaaaatc caaaccatgt aaacaatcgt tcctatcaac gcttgcctgc
     8881 ttccccaaag tcctaaagcg tagatgatta gcggtgcgct gaacactagc gctctattaa
     8941 attttcccat ccgcttgcct ccttaagatt tccgaaaaat ttgtttctaa aaattcaagt
     9001 gttttacttc ttaaaaatag atatgtgtct cttccttcaa ctggataata gacaaatcca
     9061 tttttgtttt tctcgatatc gataatattt ctatatcttg ggttctttaa aactctagaa
     9121 gtaaaccaat catattttct gttaatccgt tctagcactt ctggcaacgt catccatcta
     9181 ccagtatcat cagctttttt taactcctca taatccactt gggagataat tacatagcct
     9241 tctggaattg ggatttttgc ttctagatat tgcattagct gttcctccct ttacaattcg
     9301 tacatagtga taatcgaatc tataattctg tttgcttctg cagaagtctt tttaccgttt
     9361 aaaattaaag ataagtagct tttgctaatt ccaaatcttt cagcaagcat ggtgtaagtt
     9421 aagaactttg aactttcgac atattctttg attttttctc tatctcgttg agtgatttct
     9481 gcaatgtcag tcatactaaa actcctttct aaccaatttc ctctaaatcc atttgagggt
     9541 aatatccttc tttttttagt aattcgtaga taaatagacg ccctttctgt gtccatttgg
     9601 tattcattac aattttagtg ccaccatcgg ctttcgggat ctcagttgta tgagattttg
     9661 tgtatccttg gttcatgtgt tttttgcaca ataaccattg gttaccgact tttttctgaa
     9721 tacctagttt atgaagtaat ttattcatct gttgtggaga catcccataa tctgctgcaa
     9781 tctgactaat tgttactgaa tctgtagaag ataatatgct atctaaatag gagattttag
     9841 gttcgtactc tgcaatcttt tgttctgcga ttaatcttcc agttctttct tcttttagtt
     9901 gagttgctaa ttgaatgatt gtatctggat taagcaaagc ttcttctact ttttctggag
     9961 ttagataacc tccatgtttt ctaattgctg gcaacacttc acttgttacc caacgtttaa
    10021 attttttggc agagggaagt tttgatttta agattaaact gtacaaacca gattcgttga
    10081 tgattgtcat ctctcttgat tgacctgagg tcgtgattcg cgaccccatc ttatcttcca
    10141 aatctacatg ccgcgacaaa gcatctttag tgtttgaata acccaaaaca ctcgcaacgt
    10201 ctttgcctac aaaatatggt tcatcattta ctaaaattgt ccgaacttcg ttttgttcga
    10261 aattaaaaat ttgcggtgtg ttcatatttc tcattccttt cttttatatt cgtaaacaaa
    10321 tttaacaact tttttctaaa ttcaattgac agtatctgag taatactcta tactaagagc
    10381 ataattaaat aagacataaa aacattgatt ttaaagcttt cttggcggtc tgcatttaca
    10441 tatcaatagt gttttttgtt gtctttttag ttgttaaatc tgtttacaag atatagtata
    10501 gctcattact cagaattagt caaattattt ctgcgtattt ttctaaactt tttttgtaaa
    10561 catttagaaa ggttgtttta tcgatgaata cctatgaaat aataaaagac ttggcgaaga
    10621 ggaaaaagat atctatacgg cagttagaaa ttaattttgg atattctaac ggttatctag
    10681 caaagtggaa aaataatact cctaattcag atgaattaag tcggattgct gactatttcg
    10741 atgtctctgt agactacctt ctaggtagag aagaaagaga aacccctaaa catgtggatt
    10801 tatcagaaga cgatactgtt ttttcttttg atggaaaaga aatatctaag gagacaatgc
    10861 gtaaagcgat tgcaattgct aaagctttag aggaaaatga atagttggag tgatggtttg
    10921 tatgtatttg aaattgaaag aaatgttgag cgaatacaat ttgaaagtag tttatatgga
    10981 gatgaaagag cctggtttct actatccaaa acccagaatc atttttttga atgaaaatct
    11041 atacggtgaa actgcagaag cctttcattt atctcatgaa cttgcacatt tcagcgcctc
    11101 tcattttgaa ttttcagttt tgtacgatac ttctacaact tttcactcaa aatttgaaac
    11161 agaagcagat aaaatagcta ttctaatttt attaaatatc tatattgaga acgaactgac
    11221 tgatgaatct cagtttaatc tcgaaaaatt tatggagtat tattctattc agaataagct
    11281 cagatacact tgttatgctg tctgccaatg ctattttaag aaaaaatatt cttacgcaag
    11341 gcaatatgta tgaatacatc aagaattatt aaaaaaaccg atttcagcaa aacattaaaa
    11401 aagcccgtgc tgcaacacgg gctctttcct catatatgag cttctacaaa aaaaatcata
    11461 tcacagaaat gaggaagaaa aaatggaaaa agagatttta ataaaatctc caggaaaaac
    11521 cttgattaaa gtaactgatg attctatttc aattattaga aaaggattta taaatttagt
    11581 taatcaaggt attaaaggag aaaaaactat cccctttaaa aatatatcag ccgtacaatt
    11641 aaaaaaacca ggtatgagta atggttatat acaatttacc ttgctaggcg gaaacgaaag
    11701 tcgcggaggt atattagcgg ccacgaaaga tgaaaacacc gttatgttta caaaaaaata
    11761 ttggaatgaa atggaaaatt taaaaaaata tatagagaag caacaatcta tattagataa
    11821 taattccaaa gaaacacaaa tttcaagtgc agatgaaatt aaaaagtata aagagttgtt
    11881 agatgaaggg attattaatc aagaagaatt cgattttaag aaaaaagaat tgcttggttt
    11941 ataatattca atatcaaaag agaaagagga tctattaatg gacggtatat ttggttttac
    12001 tggtattgtt ttatttatta taggtttaat catgttaatt gtaagattta tcaaaaaaac
    12061 aaataagaaa acacctatga ttctccttat tatcgggatt atttttacta ccgtaggttt
    12121 ctcgctttcc tcatcaaacg aggcaaatga caataacaaa caggaaagct catcatctga
    12181 tactcaaact actacatcta acagcagcaa taaaagccta actcaatttg aagaattttt
    12241 agagacaaat aatcatgact ggggtgcttt cttagatagt tattattcta ttactccagc
    12301 tactgagcaa aatacagctt ttactaaata tattagtggt aaaacatata cttttgaagg
    12361 aactgttatc gaatcaatga ctactagaat agccattata gcagacaaag agtacgatga
    12421 taaaagttgg aatgatattt ctgcaacacc taaagtttca tatgtaattt ttgcgaagga
    12481 cttaaacaat gctgatacct ttacaaaggg cgataaagta acttttacag gagaaattag
    12541 ttcaagagga tcgaacatag aaaaatccta tgctcaatgg gatatgatta atagtaaagt
    12601 aagtaaaaaa taaatgaacc acgccccact aagagttaga ttgtgcacaa taataaaacc
    12661 tatacaaaag agtgccatca ggagtcacaa tgcttgtaaa ggaaatgaca aaataggagg
    12721 taactatgga taatagcaat gaaaaactaa ccaaaaaaga atatcttgaa ttaatagttg
    12781 aaggcggtat tggagcaatt ccttatattg gtggcgttgt gcagtcactt tattttgggt
    12841 ctaaaaacga gaagcgtttc aaacgcatcg agaaattcta caaaaattta aacgaatcat
    12901 tagaaaacat tcgcgatcaa attccagaag atgttttcaa cttagagaac aaggaccaac
    12961 tcattggaat attcgaaggg ataaatgacg aggtagaaaa atcaaaggca caaaataaaa
    13021 tagatatgta taaaaatctt tataagaatt gtttacttcg aattaacaac gcttcatggg
    13081 ataacgagga gtattatctt cttgtactga atcaattaac atcgattgag attcaattgt
    13141 tagcatgctt aatgagtcgt ggaaacgaaa atttcactgg aaatatcagc atgtctggct
    13201 attcacaaga gctaatagat ggtagcttaa atagattatc tgattttggt ctgttagaaa
    13261 aaacgattag ctccatagta ttaggtgatg tcggcaaaca aaatatgggc tacaaaatat
    13321 ctcatttagg aattcaattt attaactcaa ctcttactta agttttcttt ctaatatgac
    13381 atctagacct atcagcaatg caacaataaa aaaccagaca taccaataat caacaaataa
    13441 tctaaactct ggaagttcag ttattctttt aaaaatcact ttaatcataa taaacacctc
    13501 tgattggatg atctaataca catccaatta taccattaaa acgacaaata gcacatcctt
    13561 gcgccggcaa gcaaaaagga tgtgcaaaaa ataataaaac ccaaacaatg ggcttcttta
    13621 tagttcctat tgtatcagag aaagagagct gattcaatta tggcaagatt agtcaaacgt
    13681 ggaaatagtt ggcaatacga aatttcatac aaaaaagatg acggaaaata cacgaagata
    13741 agaaaatcag gatttaagac aaaaggagaa gcaaaagatg ccgccaacga attagaatat
    13801 aacttgaaca aaggccttaa aggggatcgc aaaaatctat tattatcaga ttactttgag
    13861 gattggatgc aactttataa agaaggaaca gtatctccta tcacttatag aaaatacgaa
    13921 gatacgttaa tgaacataaa gaaatatatg ccagcggtat tgatttctga tttagataga
    13981 gtgggatatc aacgcttttt aaataagtat gctaaagacc atgtaaaatc taccgttatt
    14041 aagtttaata accatattag agcgtcgttg aaagatgccg tagaagaagg attaattccg
    14101 tttgatccaa ctagaaaagc agtaatcaaa ggaaaagatt cattgaagcc aaaagaagat
    14161 aaatatttag attatgatca atttaaatct ttaatgaaac tcgtagaaga aaacctttct
    14221 gcgcagtact cttctcctat gctcgtgtta gttgctggtg ccactggaat gcgatttgct
    14281 gaacttctag gattaacatg ggaagatatc gatttcgaag atcaaatcat cactattaat
    14341 aaaacatgga attataaatt aaatgaatgg ggaaaaacaa aaaacgaaac ttcaaatagg
    14401 aaaatttcca ttgataaaca tacgattgat ctcttaaaaa agtttaaaat caatcaaaaa
    14461 gaattattcg agaattttga aatcaaaaac cctcataatt ttgttttttt caacttaaaa
    14521 aatggattag tttcatcaaa cgccgtcagc aaatatttgc gcaaaaaatt aaaagaatta
    14581 gggattgaaa agcaatttac tttgcatgga ctaaggcata c
//
LOCUS       DAZKMK010000070        14219 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000070 DAZKMK010000000
VERSION     DAZKMK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.071"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8V1_002026"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8V1_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0330113.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8V1_002027"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8V1_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0330114.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8V1_002028"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8V1_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0330115.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8V1_002029"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8V1_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330116.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8V1_002030"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8V1_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0330117.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8V1_002031"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8V1_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0330118.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8V1_002032"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8V1_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330119.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8V1_002033"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8V1_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0330120.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG8V1_002034"
     CDS             complement(7136..7417)
                     /locus_tag="ACG8V1_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0330121.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG8V1_002035"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8V1_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0330122.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8V1_002036"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8V1_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0330123.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8V1_002037"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8V1_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0330124.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8V1_002038"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8V1_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0330125.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8V1_002039"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8V1_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0330126.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8V1_002040"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8V1_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0330127.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8V1_002041"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8V1_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0330128.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8V1_002042"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8V1_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330129.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14219
                     /locus_tag="ACG8V1_002043"
     CDS             13982..>14219
                     /locus_tag="ACG8V1_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330130.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEG"
BASE COUNT         3936 a         3057 c         2240 g         4986 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggat
//
LOCUS       DAZKMK010000071        13978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000071 DAZKMK010000000
VERSION     DAZKMK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.072"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..461
                     /locus_tag="ACG8V1_002044"
     CDS             141..461
                     /locus_tag="ACG8V1_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330131.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="ACG8V1_002045"
     CDS             913..1113
                     /locus_tag="ACG8V1_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0330132.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1260..4049
                     /locus_tag="ACG8V1_002046"
     CDS             1260..4049
                     /locus_tag="ACG8V1_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0330133.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4096..4623
                     /locus_tag="ACG8V1_002047"
     CDS             4096..4623
                     /locus_tag="ACG8V1_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0330134.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4644..5834
                     /locus_tag="ACG8V1_002048"
     CDS             4644..5834
                     /locus_tag="ACG8V1_002048"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0330135.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8V1_002049"
     CDS             5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8V1_002049"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0330136.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7967..8641
                     /locus_tag="ACG8V1_002050"
     CDS             7967..8641
                     /locus_tag="ACG8V1_002050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0330137.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8638..8979
                     /locus_tag="ACG8V1_002051"
     CDS             8638..8979
                     /locus_tag="ACG8V1_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330138.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9009..9368
                     /locus_tag="ACG8V1_002052"
     CDS             9009..9368
                     /locus_tag="ACG8V1_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0330139.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9628..9750
                     /locus_tag="ACG8V1_002053"
     CDS             9628..9750
                     /locus_tag="ACG8V1_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330140.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9892..11976
                     /locus_tag="ACG8V1_002054"
     CDS             9892..11976
                     /locus_tag="ACG8V1_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0330141.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12287..13753
                     /locus_tag="ACG8V1_002055"
     CDS             12287..13753
                     /locus_tag="ACG8V1_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0330142.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4636 a         2430 c         2974 g         3938 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1201 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1261 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1321 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1381 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1441 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1501 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1561 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     1621 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     1681 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     1741 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     1801 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1861 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1921 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     1981 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2041 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2101 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2161 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2221 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2281 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2341 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2401 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2461 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2521 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     2581 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     2641 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     2701 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     2761 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     2821 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2881 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     2941 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3001 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3061 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3121 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3181 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3241 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3301 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3361 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3421 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3481 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3541 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     3601 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     3661 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     3721 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     3781 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3841 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     3901 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     3961 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4021 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4081 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4141 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4201 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4261 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4321 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4381 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4441 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4501 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4561 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     4621 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     4681 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     4741 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     4801 tcgaagcaat ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc
     4861 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4921 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4981 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5041 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5101 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5161 catcgaatcc gactggaatg atttatagca agaatgaact tcaggcaatc ggtgaatggg
     5221 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5281 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca
     5341 tcaatggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggga
     5401 attctgagat catcaatgga atgattgctg ttgcttccca gtcaaccagt aatccgacag
     5461 cagtcagtca atatgcagct gttgaagcat tgaaaggtga gcaagataca gtagaagaaa
     5521 tgcggaacgc ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccag
     5581 gtgtttctct tgaaaagccg caaggagctt tctatctttt tcctaatgta aaagaaacgc
     5641 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     5701 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     5761 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     5821 aaagtcaaaa ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5881 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5941 tcgcaaataa gcgatcaagc ggaaaaatca catttttaca attaagagac ggaacagcct
     6001 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6061 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6121 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6181 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6241 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6301 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6361 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6421 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6481 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6541 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     6601 tagcattctt agtacaaaat gttttagatc attgcgaata cgcattagat gtactaggac
     6661 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttacgatg
     6721 atgcaatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     6781 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6841 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa
     6901 tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6961 caacggacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7021 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7081 gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7141 tcccacgttt gttgaaccga atttatccat aagaggttgt gcgcctgccg ttcagctccg
     7201 agtcacaagg agtaatttca aaaaacagtt tttcatgttt gatgactatt gtgacttgtg
     7261 gccggagagc aacgtttatt taaaaaaaag aactgcgaac tctcgcagtt ctttttttta
     7321 aatacgggat aatggaagtt tccagcagac aagagaagaa agcaaaagaa attgctattt
     7381 gattcacaag gttctataac gaaatcgcaa tcaatcaaac atttacaaag aaggtaattc
     7441 aaatggaaat ctgatttgat gatgcttctg ctgttgaaat aaagcagcat ctagcacctg
     7501 aaaacaaact aatagtaaca ttcgaagatg gagtaggtcc gtattttcag catgcaataa
     7561 tccatatgta agttcaattc acccttgaaa tcatcggacc agataaagtt atgaccaatc
     7621 tatcccaagc aatctagaag agatattagt aaagggacat ttactagaag tcttagatga
     7681 gcctataaat atatgtttcg atggcgtcat cagtggtctg attgacggaa gcttgggtgt
     7741 gatcgatctc tctggaccaa acaacgaagg aatcaagggg aatccggcac gttaagaaaa
     7801 aaatagcata aaaaactaga cacttgatga gcgttcattc aattgtttga aggaactagg
     7861 ctggtgtcat tttttataca tatattttct ctttcgtctt gatagggctt atgggataat
     7921 ataaggaaga aaatcatcgt tttattcaaa aaggagtttt tgacaaatga aaaaacgtat
     7981 attatttatc ggtgatagca ttacagacgc aggaagatac cgagcagaag caactgatct
     8041 aggtaaaggt tatccacttt tgattgccgg aaaactaatc tcccaatatc caacgatgga
     8101 atttgaatgc atgaaccgtg ggataggagg agacaaaatt gcagatctgc aagtacgctg
     8161 gcaagaagat tgtttagctt tacaacctga ctatgtatcg attttgatcg gaatcaatga
     8221 tacctggcat aatactagcg atcaggctat attcggaact gagaaatctg ctcagcaatt
     8281 tgaatccgtt tatcgaaaat tactaaatga acttcaaaca gcggaaatct gcaacatcat
     8341 cttgatggag ccgtttgtac ttcctataac cgacgaccgt catggctggc gtaaagactt
     8401 agaccctaaa atccaaattg ttcgtaaact tgctaaggaa tatggcgcat tgctggttcc
     8461 tttagatggt atattcaatg ctttagggat tgcttatggt ttttcgaaat atacaagaga
     8521 agatggggtc cacccaacga ttggcggaca tgagatcatt gcacaaagct ggatagatgc
     8581 aattgtcgca gtcgattttt ttagtggaga taaaaatgag cgaaagaaaa gtgaaaaatg
     8641 atggaaataa ggtgggcagt aaaagaagat ttacctcaaa tcaatcagct gattactgat
     8701 catccagaat cattcgtaaa agcaaaaacg gcagctgacc taaatgataa gaataaacta
     8761 ttgctcgttg ctgtaacaaa agaaaaacgg attatcggat ttgcgcaagc ggacgtgcaa
     8821 cagtcagaag aacgattgga tcagcgacca tcaggaaaac taatccaaca cacattagta
     8881 acaaaagaaa atacggaaga aatccaaaaa aaatttgcag aatacttgat gctggaagct
     8941 aaaaaaagaa gaataaaatc aatttttgcc cagttttaat caaaaacagt aatagaagga
     9001 gaaatacaat gagttgttta ggaaatgtca tctggtttat attcggcgga ctaatcggtg
     9061 gtcttagctg gttagcagcg gggatacttt ggtgtatcac gatcataggt atcccaatcg
     9121 gtatgcaatg ttttaagatc gcctcccttt cttttgcgcc ttttggcaaa gaagtaattt
     9181 atcaccagtc tggcatgtca ttgatcgtta atatcatctg gctgatcgtt tcaggcctac
     9241 caattgctct cgcacatgtc gtcagcggca tccttctatg tatcacgatc atagggatac
     9301 cttttgccaa gcaatctttc aaacttgctc gattggcgtt gatgccattc ggtgcagaaa
     9361 ttgtataaaa gagaataata aaggttagat aaagcataga gccaggtggg atacatgtat
     9421 ttcacctgac tctatgcgtt ttcctgtata agttcttcta tgtgactttt acgtctttta
     9481 tttatatggg gttactgttt atggaaagtt gaaataattt tcattaaaga actctttcta
     9541 tatttttgaa agcgtagtgg agcttcgtat atctgggaag gacattttgc agggattgaa
     9601 aaaagatatt gtcctatcga aaaattaatg aatttagaca aattgccagc aacaggcttc
     9661 aaacttagtt gctatcctgt caagatcaag aaggcaagtg cgggttggat aagagcagtg
     9721 gcaatgatcg aagagaaaaa gaaagaataa gccaaaacat ctgtatggaa gaaattttcc
     9781 gatgcagatg tttttcattt tttctttcgg ataccttgac atatgaataa ccattcattt
     9841 ataattagtt atgaagagat attcatatgt tgaggcgagg aggtcattaa aatgaaaagt
     9901 tacagattag aaggtttaga ttgtgcgaac tgtgcaatga aaatcgaaaa aggagttcag
     9961 aaaatcaatg gagtcaagga agcaacggta aacttcactt ctggaaaact aacgattgac
    10021 gcagaggaag atcatttggc tacgattgaa caagaaacaa aaaaagtagt gaaggaatta
    10081 gaaccagatg tcaaagtaac agaaatagat aaggaaaaag tatctgaaca tggaaatgaa
    10141 aaaaagagaa atacgctgtt ccggatttta ttttcccttg caggtatcgc tttattgcta
    10201 ttatttgatt ttaatgaacc aattcgcttg atagggtatt tattgattta tctgctgata
    10261 ggatatgatg tagtgaagaa ggcagtgatg aatatcgtca aaggaaaaat ctttgatgag
    10321 aattttctta tgtctgttgc tacgattggc gctatgttga tcggtgaata tccagaagcc
    10381 gtagcagtga tgctatttta tcaaatcggc gagtatttcc aaggattggc agtcagtcat
    10441 tcaagaaaat cgattcgcga attgatggcg atccgtccag aaacagctca cgtgcagacg
    10501 gcggaaggat tgatgaccgt gaaccctgaa gacgtgctga tcggtcagtt tgtacttgtg
    10561 aagcctggtg aacgtgtacc gttagatgga acgatcatcg aaggagaatc attagtcgat
    10621 acgtcagcac ttacaggaga atcagtccct aaatctgtat atgttggtga aacagtatta
    10681 agcggattta tcaataaaaa taaaccattt ttagttcaag tagaaaaatc ttatgaaaat
    10741 tctactatca gcaaattatt agaattagta gaaaatgcca gcagtaaaaa agcaccagct
    10801 gaaaatttca ttactaaatt cgcgcgctat tacacaccag tcgttgtagg attggccgtt
    10861 ttattagctg ttttacctcc acttgttgtt tctagagcgg cgttttctga atggatctat
    10921 cgagcattga catttttagt gatctcatgt ccatgtgcat tagtgatttc cgtgccactt
    10981 agtttctttg gcggtatcgg aggagcaagt aaaatcggtg tattagtaaa aggttcaaat
    11041 tacttggagt tgcttgcaca aactgagaca gtggtattcg ataaaactgg cacattaacg
    11101 aaaggtgact tttcgatcca gacgatcgat ccaacaggaa ttgatccaaa gaaatttatg
    11161 caatatgttg caagtgcaga acagttttct acacatccga ttgctcaatc tgttttagaa
    11221 ggctacgcag gtccgctttt accaacagct aatatccagg aatttgcagg tgaagggatc
    11281 ttagctgaag ttgatggcaa acaagtatta gtaggtaatc acaaattgat ggaaagattt
    11341 gaaatctctt ttccttcaag tcaagaaatc ggtacattgc tttatctagc gatcgatcaa
    11401 agttacagtg gttatcttgt tattgcagat accttgaaag aagatgccgt agacgcattg
    11461 gttcaattaa aacaggctgg tgtgaaaaac acagtcatgt tgacgggcga ctcgaaaaaa
    11521 attgctgatc atatcgggaa gcaagtaggc gtggacaaga tatatagcga attgcttcca
    11581 gaagataaag tgcagcggtt agaggaaatc ttacaaagta acaataaaaa gaccgcattt
    11641 gtcggagatg gaatcaacga tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg
    11701 ggtggattgg gaagcgatgc tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa
    11761 ccatcgaaga ttgcagaagc aatccattta gcaaagaaaa cattgaaaat cgtcaaacag
    11821 aacattgtct ttgctattgg gataaaaatt ttggtacttt ccttaggtgc ttttggtttt
    11881 gcatcaatgg gagatgcagt atttgcagat gttggggtga cagtattagc tgttttgaat
    11941 gccatgcgaa gcttgcatgt aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt
    12001 cctctttttt ttatatcctt ttcttaatga aaaatgtaaa gaaaatagat aggattataa
    12061 aataactaat gtttttaatt ttgcagaaaa ttcaaaatag cttgaaattc aataaggtca
    12121 atgaaataaa agaaaataac ttattttttt gatggaaaaa atatgaaaaa acgatgtgta
    12181 ttaaataaaa gaatacacac ataaaataac actgtcttgt aagtttgtta aaaattgaat
    12241 atgattacat ctggataaca aatttttatc ataaaggaga gaacgaatga acaacaaaca
    12301 attacgctgg tttactgttg cgttaattgc atttaacatg gtttggggca tggggaatgt
    12361 agttaataac tatgctcagc aaggagtatc agttgtcacc tcttggctat tgattttggc
    12421 catttatttt attccatacg cattgatcgt tggacaatta ggatcagcct tcaaggatag
    12481 taaaggaggc gtcagttcat gggtcgaaaa tacgacatct aataaacgac tggcatacta
    12541 tgccgcttgg acttactggg tcgtccatat tccttatttg gcacaaaagc ctcaagcaat
    12601 tctgattgct ggtggttggg cagtccaagg aaatggaaat atcgtaaata cgatgtcagt
    12661 acaaatggta gcagttattt cacttattat atttcttgcc tttttatatt tatcaacaaa
    12721 aggactttct actttgaaag tgattggcgg actggccgga acagcgatgt tcgtcatgtc
    12781 gatcttattt attcttttag ctgtaacggc accgttcatc aatccaacga tggaatttgc
    12841 cacaccacat atgaatcaag tgaaaacata tattccaaca tttgatttca gttatttcac
    12901 aaccgtttcg atgctagtat ttgcagttgg aggggcggaa aaaatatccc cttatgtcaa
    12961 ctctacaaga aatcctgcaa aggaatttcc taaagggatg atcttcttag cagcaatggt
    13021 agggatttgt gctgtattag gctcagtcgc aatgggtatg ctgtttgcta gcgataatat
    13081 ccctaaagac ttgatggcaa atggggcata cagtgctttt caaattttag gtaattacta
    13141 tggtatcggt aactcattga tgatcattta cgcattgaca aacatgattg gacaaatctc
    13201 cgcattagcc ttttcaattg atgcaccact aaaaatcttg ttagcagatg cggatccaga
    13261 atttgtacca aactggctac gtaaacgtaa tcgtaaaggt acattggtga atggttatct
    13321 tctgacaggt atactagtag gaatcattat tttgttgccg atctttggta tcaaagatat
    13381 gaatgaatta gtaaaatggt tgacgaactt gaactcagtt gtcatgccaa tgcgttacct
    13441 atgggtgttc ttggcatata tgatgttgaa taaagcttat aagaaattca attctgaata
    13501 taaatttgtt aaaaatccaa aaatcggttt tatctttggt gcttggtgct ttgccttcac
    13561 tgcgtttgca tgtatcctag gaatggtgcc aaaagtagat tatactgctg atcctaaagc
    13621 atggtggttc caattattca cgaatatcat cacaccaatt gtcttgatct tattaggtat
    13681 gatcttgcca gcaattgctc gtcgagacaa aaacaaagaa attactgaac cagaaacagt
    13741 ggttcagcct taaaatagaa atacttgtta caagaggttg tgacagaagc tacttctgga
    13801 acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
    13861 caaaaatagc tcctcatatt ttgccgtttt ttgagctaat gctgaaggtc agctgattga
    13921 acaccattta ttcgaaacaa gggactttga cacgactttt gtcacagtcc cttgtttt
//
LOCUS       DAZKMK010000072        13949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000072 DAZKMK010000000
VERSION     DAZKMK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.073"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..125
                     /locus_tag="ACG8V1_002056"
     CDS             <1..125
                     /locus_tag="ACG8V1_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0330143.1"
                     /translation="NQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(303..2300)
                     /gene="tkt"
                     /locus_tag="ACG8V1_002057"
     CDS             complement(303..2300)
                     /gene="tkt"
                     /locus_tag="ACG8V1_002057"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0330144.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2402..2653)
                     /locus_tag="ACG8V1_002058"
     CDS             complement(2402..2653)
                     /locus_tag="ACG8V1_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0330145.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2800..3423
                     /gene="lexA"
                     /locus_tag="ACG8V1_002059"
     CDS             2800..3423
                     /gene="lexA"
                     /locus_tag="ACG8V1_002059"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0330146.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3603..3821
                     /locus_tag="ACG8V1_002060"
     CDS             3603..3821
                     /locus_tag="ACG8V1_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330147.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4110..4622)
                     /locus_tag="ACG8V1_002061"
     CDS             complement(4110..4622)
                     /locus_tag="ACG8V1_002061"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0330148.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4636..6933)
                     /gene="recJ"
                     /locus_tag="ACG8V1_002062"
     CDS             complement(4636..6933)
                     /gene="recJ"
                     /locus_tag="ACG8V1_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0330149.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7075..7518)
                     /locus_tag="ACG8V1_002063"
     CDS             complement(7075..7518)
                     /locus_tag="ACG8V1_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0330150.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7519..8307)
                     /locus_tag="ACG8V1_002064"
     CDS             complement(7519..8307)
                     /locus_tag="ACG8V1_002064"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0330151.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8304..9245)
                     /gene="rnz"
                     /locus_tag="ACG8V1_002065"
     CDS             complement(8304..9245)
                     /gene="rnz"
                     /locus_tag="ACG8V1_002065"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0330152.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9422..10009)
                     /locus_tag="ACG8V1_002066"
     CDS             complement(9422..10009)
                     /locus_tag="ACG8V1_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0330153.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10230..11543)
                     /gene="obgE"
                     /locus_tag="ACG8V1_002067"
     CDS             complement(10230..11543)
                     /gene="obgE"
                     /locus_tag="ACG8V1_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0330154.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11831..12841
                     /gene="gap"
                     /locus_tag="ACG8V1_002068"
     CDS             11831..12841
                     /gene="gap"
                     /locus_tag="ACG8V1_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0330155.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12926..13861)
                     /locus_tag="ACG8V1_002069"
     CDS             complement(12926..13861)
                     /locus_tag="ACG8V1_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0330156.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3859 a         3123 c         2453 g         4514 t
ORIGIN      
        1 tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct agaaaatcac
       61 taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag ttttgtctcg
      121 cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat tctttgtttt
      181 gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc ttctaacaca
      241 taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac aattgcttgg
      301 atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc aaattcttct
      361 aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct
      421 gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac acgtttcttc
      481 actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa gaggtcaaag
      541 cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt ttgagctttc
      601 acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt ttctccttgt
      661 gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc ttttgtagaa
      721 ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc tgttgtctcc
      781 atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat cacttgaaca
      841 ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct
      901 ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg gattgctgct
      961 aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc gtagatacga
     1021 gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc gcggacaccg
     1081 aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc agccaccatt
     1141 gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat agctttcgat
     1201 aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc aacttcataa
     1261 gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc ttcaaattgt
     1321 tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca tgcatcttca
     1381 gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc ttctggaaca
     1441 gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc atcttcacca
     1501 agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc gatgactgtt
     1561 tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat cgctttagag
     1621 attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata tgcttcatag
     1681 cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga aatatcgttt
     1741 gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga acttgcttct
     1801 tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata gtgatccatc
     1861 acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat tgccataccg
     1921 acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc atctgtatga
     1981 tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt caaatcatcg
     2041 attgtcactt gatagccagc taaatgaagc aagctataca acattgctga cccatgtcct
     2101 gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg atttactttc
     2161 aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa tccaggatgt
     2221 cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt aacgccaagt
     2281 tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt ttacctttat
     2341 ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc agtatttttt
     2401 actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct tttaattttt
     2461 ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata tgatgacgca
     2521 taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct tgttcttcgg
     2581 cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc tctatttttt
     2641 caggtgacag catgctcagc cctcctttta ctctccttca tattatataa aaactgaaaa
     2701 aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc gctttttata
     2761 tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca aactgaatca
     2821 agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg atacccgccg
     2881 actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt tcacggtcat
     2941 cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc tcgtgcaatc
     3001 gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat tccagtactt
     3061 ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga cttctttcca
     3121 atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat tcgtggagat
     3181 agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa gcaagaaact
     3241 gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac ttgcaaacga
     3301 ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct cgaaccaatc
     3361 atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag tcatatctat
     3421 taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg gtctcaaact
     3481 cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt tacaaaccga
     3541 acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag aggtgagaat
     3601 tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta ggtattatta
     3661 ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt actttgtggt
     3721 tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt catgaagatc
     3781 atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg actgctaatt
     3841 attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt cacatccttt
     3901 ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat tgcggaaaaa
     3961 gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa atagcgaccc
     4021 tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct tctttaagaa
     4081 taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat gatgtcatat
     4141 cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc gcagcctacg
     4201 actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc tccagtagct
     4261 agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt atgcaatgtc
     4321 aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg aggtaacttc
     4381 ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg acatcctacg
     4441 atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt tgcataattt
     4501 acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg ggaaatatct
     4561 cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc ttttaaatcc
     4621 actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat cgctcaaaag
     4681 caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc tctcagtcaa
     4741 aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg taacaaattt
     4801 caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac gcaaatattg
     4861 agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga tcaatttaaa
     4921 caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat caggtgaaat
     4981 ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca gatccgctaa
     5041 atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa ttattgcctt
     5101 tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag gcacgaattt
     5161 caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc tctgttcacg
     5221 gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat cagataccat
     5281 cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta ccacatctac
     5341 ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg catcaagctg
     5401 actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga ttcttttgat
     5461 attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg
     5521 cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca gcaatacttc
     5581 atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta tatattggtt
     5641 catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga ctgctgcatg
     5701 atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa aaagattcaa
     5761 ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag taaggacaag
     5821 cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa cgccttcatg
     5881 ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg ctaatgcttc
     5941 ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca atttttttgc
     6001 ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag gacgtggatc
     6061 gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc
     6121 tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta tccgttctga
     6181 atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag tcaatgaaac
     6241 taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg gttcttccaa
     6301 taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa atggatagtg
     6361 ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct caggtagttc
     6421 gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta ccgcatcatt
     6481 tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga tcttttcttg
     6541 gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga atacttctac
     6601 ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga tcccatctgc
     6661 gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt ggattcggct
     6721 gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt cctttggatg
     6781 taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta acttaccgac
     6841 aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag gctctgtctc
     6901 gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt agttattata
     6961 gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca gatccgctag
     7021 acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc cctattagtc
     7081 aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg ctttcaattg
     7141 actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca actccgcacg
     7201 ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct tttgaagttc
     7261 tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt gctgccatag
     7321 tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac cgatgatcac
     7381 taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat ttttattgtt
     7441 caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca cattgctttg
     7501 ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat aatcaccaat
     7561 cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt gattgatttc
     7621 ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag gttctaaaac
     7681 gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga agaaatttgt
     7741 ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca attctaatcg
     7801 caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg ttgattttgc
     7861 agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc ttctttctgc
     7921 catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga cttcaaacat
     7981 atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt
     8041 gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata attcgtccac
     8101 gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc tcaagcgtct
     8161 gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta taacgattgc
     8221 tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac tacctgttac
     8281 taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg
     8341 ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc
     8401 tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag ccgttttgcc
     8461 tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc
     8521 attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct attttccgca
     8581 agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt
     8641 ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag ttggatcgtt
     8701 tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc ttttagacga
     8761 tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa actgtcgatc
     8821 ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat cggggctgtt
     8881 tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat acctaaagag
     8941 acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt atccccacct
     9001 tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt
     9061 gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc
     9121 gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag tttcaatgct
     9181 atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa aaattgtaat
     9241 tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa aagaagggat
     9301 aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt
     9361 ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct gctgtttttt
     9421 attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc
     9481 aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca tgcacatctt
     9541 tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc attaccaatt
     9601 gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta gctccttcac
     9661 cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct ttggcatctt
     9721 gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc ggaccaccta
     9781 atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta ccggtaaagt
     9841 cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg acaactgcca
     9901 tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa aatgcctgat
     9961 atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt aaaaacgccc
    10021 ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa agaaaagcac
    10081 tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc tttttccgaa
    10141 taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc tgaatctata
    10201 ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc aaactcaccg
    10261 atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc cacacccata
    10321 ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt cgtcatttgg
    10381 aataattttt cgatcttctc accagataag atccacgttg catctgaatc acgatcgatt
    10441 tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc cagttcttct
    10501 tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt caataacgcg
    10561 tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc agcaaattca
    10621 tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg tgcatccggc
    10681 atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa gttataagta
    10741 gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc catcccactc
    10801 atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc
    10861 agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc ggcaaaactt
    10921 ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa atgataagcg
    10981 ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc gacagatgga
    11041 aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt tcgctcttgt
    11101 cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc aaaacggata
    11161 ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt ttcaattaaa
    11221 tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg tactttaaca
    11281 taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc acctggctgc
    11341 gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc
    11401 aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc aggaacgtat
    11461 ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac atcgatcgtt
    11521 acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat ttatttgact
    11581 gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat ctgattttgt
    11641 ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg gtaaggtttc
    11701 caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc gtagcagaat
    11761 aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta ataaaggagg
    11821 aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg gccgtctggc
    11881 tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca acgacttaac
    11941 aagtccaact atgttagcac aattattgca atttgactct actcatggta cttatcctgg
    12001 cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc gtgtttatgc
    12061 agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata tcgtactaga
    12121 atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa
    12181 acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata acgtcaatga
    12241 tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa caaactgttt
    12301 agcacctatg gcttatttct taaataatga atttgggatc gaagttggta caatgacaac
    12361 agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag gcggtaattt
    12421 gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg ctgcgaaagc
    12481 aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac aacgtgttcc
    12541 agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag taactgctga
    12601 tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg ggtatgatga
    12661 ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct ttgacccaac
    12721 gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg catggtatga
    12781 caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg ctaacttgta
    12841 atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag gcttctttct
    12901 tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat tgttggacta
    12961 cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct ccttttacaa
    13021 catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt tcaaatggta
    13081 caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc ttcaattgtt
    13141 ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact agactcgtta
    13201 cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa gcaagctcat
    13261 catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct ggtagttttt
    13321 ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga acgatttctt
    13381 gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct ttacgctctg
    13441 gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca tcaggaacaa
    13501 ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc
    13561 ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca ggaagataaa
    13621 tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg ttttcttttg
    13681 aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca ggtgtatctt
    13741 cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa ttccctgcaa
    13801 acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc caaagtttca
    13861 taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata atcaaagaat
    13921 ctctactatg caatgttcat ttttataac
//
LOCUS       DAZKMK010000073        13849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000073 DAZKMK010000000
VERSION     DAZKMK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.074"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..165)
                     /locus_tag="ACG8V1_002070"
     CDS             complement(<1..165)
                     /locus_tag="ACG8V1_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0330157.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADG"
     gene            complement(644..1363)
                     /locus_tag="ACG8V1_002071"
     CDS             complement(644..1363)
                     /locus_tag="ACG8V1_002071"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0330158.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1412..3058)
                     /locus_tag="ACG8V1_002072"
     CDS             complement(1412..3058)
                     /locus_tag="ACG8V1_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0330159.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3237..3827
                     /locus_tag="ACG8V1_002073"
     CDS             3237..3827
                     /locus_tag="ACG8V1_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0330160.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3919..5424
                     /locus_tag="ACG8V1_002074"
     CDS             3919..5424
                     /locus_tag="ACG8V1_002074"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0330161.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5508..6860)
                     /gene="celB"
                     /locus_tag="ACG8V1_002075"
     CDS             complement(5508..6860)
                     /gene="celB"
                     /locus_tag="ACG8V1_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0330162.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6860..7378)
                     /locus_tag="ACG8V1_002076"
     CDS             complement(6860..7378)
                     /locus_tag="ACG8V1_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330163.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7394..7720)
                     /locus_tag="ACG8V1_002077"
     CDS             complement(7394..7720)
                     /locus_tag="ACG8V1_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0330164.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7733..9712)
                     /locus_tag="ACG8V1_002078"
     CDS             complement(7733..9712)
                     /locus_tag="ACG8V1_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0330165.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9733..10047)
                     /locus_tag="ACG8V1_002079"
     CDS             complement(9733..10047)
                     /locus_tag="ACG8V1_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0330166.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10215..10508
                     /locus_tag="ACG8V1_002080"
     CDS             10215..10508
                     /locus_tag="ACG8V1_002080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0330167.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10586..11698)
                     /locus_tag="ACG8V1_002081"
     CDS             complement(10586..11698)
                     /locus_tag="ACG8V1_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0330168.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11851..12699
                     /locus_tag="ACG8V1_002082"
                     /pseudo
     CDS             11851..12699
                     /locus_tag="ACG8V1_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13052..>13849)
                     /locus_tag="ACG8V1_002083"
     CDS             complement(13052..>13849)
                     /locus_tag="ACG8V1_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0330169.1"
                     /translation="LDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVL
                     QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFE
                     IERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGA
                     DGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSD
                     IRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4324 a         2824 c         2371 g         4330 t
ORIGIN      
        1 cccatctgct gaaatatgaa aatttttgaa tgctacgaca ttcgccatta caggtatgta
       61 aaagaaaaaa atcatccaaa gcatacccgg tacagccatc aggacaagtg ctttataacg
      121 ccatagattc ataaaaaaat gattgaaccc ctttcgtttt ttcatctaaa aacccctttc
      181 gattcattcg caacgtaatt atagcgcttt caataatgag aataaataaa caaagtatag
      241 ggaaaatgaa acaaagtata ggagaggttg tgaaatcagc gttttggcct gagtattaga
      301 gaaaaaaacg ggaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
      361 agctgattga acaccgttta tcagaggttg tgaattcagc gttttggcct gagtattagg
      421 gagatcgtgt ccttactatc taactctgag tcacaaggaa caatggggac tgtaacaaaa
      481 gtcttgtcac agtcccttat tccgaacaaa cggtgttcca gaagtagctt ctacggaaat
      541 aagccgaaat ttcacaaaaa agccatccga aagtcagacg gacaagtctg cttttcagat
      601 ggcgctacaa ctactctgct tattctttta gctatccatt caattatttt tctctcactt
      661 gttctagctc ttctttggtc atgggtcgat attgcccttt attcaaatct ttaggcagcc
      721 aaaattttcc catacggatt ctttttaaat ataagacttc ttttcctact gctttgacca
      781 ttcgcttaac ttggtgaaac tttccttcat gcaaaatcag acggataatt gaagtttctg
      841 tctcttgatc tacttgatca atttggagtt ttgccggcaa tgtctgctca tcgccatcaa
      901 taatcagtcc ttctgcaaat aaacggatat cttcttctgt cattataccg ctaacctcag
      961 ctagatattc tttctccaca tgttttttag gtgatagtaa ctgatgggca aacaccccgt
     1021 cattcgttaa aatcaagagg ccctctgtgt ctttatctaa tcggccgact ggaaaaagat
     1081 cttcccgata atcctggtct tccaataaat cgattacggt ttgatcatga ttatcctgag
     1141 tagcagaaat gactcctgca ggtttatgca aaatgtagta aaaatctttt tgatacgtaa
     1201 tgatttctcc taaataagta accgcatctt cgaattcatt tacttgcgct ttatcgcttt
     1261 tcaccacttg tccattgact tcgatcgttc cttttttgat taatcctttg acttctttgc
     1321 ggctgccaag tcccatatct gctaaaaact tatctaaacg catgcgtttc ccccatttca
     1381 aaagagcatc aatctagctc ttctgattca cttattttat ttttaattta tgtcgtaaac
     1441 gcgctattcc tggtcctagt agtctttctg ccaagcggat tttcaaagct aaataaccat
     1501 acactaaagc ccctatacct gctactacaa gaatcaacaa tagagactgg aatttgctat
     1561 cttcgtttaa aaaagcacca aaaatcatct ttgtgatcca cgaagccaaa agcataatca
     1621 aggtaagaat aaagatcagc aatgtcctgc gccagacaaa acttggttta aagcgggcta
     1681 cttcatggat tctctttaag atcaaggtac aagacacagc aaatccgatc attgtcgcca
     1741 ataaagggcc gtatacttca aataaacgaa tggctgggta ttgaagaagg agtttcacta
     1801 gaaacccaat aaccaaataa ttaattgccg ccttgttttc aaacattcct tgcagcatgt
     1861 tggaaactag catatatagt cctaggaaga gtcctacaaa acttgcttgg atcagtacat
     1921 tacttcctag agaatccggt gtataaaata atgtatttag cggataggca agtaacataa
     1981 caccaaatgt cgccggaaac ataaaaaatg aaaacaactg taaatttccg cttgtcaatt
     2041 ttgccagacc tactcgatct ttcaacgtaa cagcttctgt aatcaacggt aacccagtag
     2101 acgcgatcga tgttgccaat gcaataacta ccatcgtcaa cttatctgga ttggcactaa
     2161 aaatcgaaaa aagatccagt aactgtgcat tggaatactc ggtggatgca gacatgatct
     2221 tcataaaagt gaactgatcg actaatttga agatcgttac ccctgaaccc acaataataa
     2281 acggaatcgc ttctttaacg gtatctaaca acaattcctt cgttgcaata tttattttgt
     2341 tttcgctgta gtgaataaac gccgatgtat acgcttgatg tttcttcaaa aaatacaaaa
     2401 ggacaagaat acttgctagc ataccgataa atgccgcgaa agttgattgc gtgaccgccg
     2461 tcacatagtc accatccatg actttcatga tgatgaatgt agccaatagc atataaaaaa
     2521 tacgtgccac ctgttctaca atctgcgaca gcgcataagg catcatttct tgattgcctt
     2581 gcaaataccc tcgtatcaca ctcatacaag gaaaaaccaa tacagcgata ctcaaagaac
     2641 gcatcgttgg aatcagttct tctccgccac cagaagcatg agctagccaa ggagaggcga
     2701 tgtacatgaa caaagcaaac aacaggccta acccgcccat catctgtaat gcacggataa
     2761 ataatcgtcg tgaagtgcca tattcattca atgaattata gcgtgcagtt tgttttgcaa
     2821 tcgcagcagg tattcctgct gtagagatca gtagaaacaa tgcatatata ttgtatccca
     2881 tattgaataa tccattggca gccttagcat tttcgcccat ccaagcatac cagggaatga
     2941 tatagacagc gcctagcaat cttgaaatga tattactggc ggtcatccaa gcagagcctc
     3001 gtgccatttt ttcttgcggt gttaattgat ctaatgctgg ttttctttga ttatccatcg
     3061 gtcagattcc aactttctac aaaacattac tatcatttta attggtaaaa cagaaactgg
     3121 caatcgattt tatataactt ttataagaac aattgttttt ttgaattcgg tttcgttttt
     3181 ttggtaaagt aaagaaatat acagcctttt atggctaaca tttataggag atatcaatga
     3241 cacaagaaat aaaaaaacta cccgttatat ttttaggtct tttctttatc acagttggat
     3301 tgaattggtt cttgctccct cacgatatag cttccgccgg agtgggcgcc atcggacatt
     3361 tagttgaaac ggccttttct atccaacgct cagttactgt ttggagcatc aatcttatga
     3421 tgctgcttct agccgctgtc ctattaggaa aatcagtatt ttttaagact gtagcgggaa
     3481 gtctgctttt tcctgttgtt ttagagattg ttccaaaagt ggaaatgatc tcctcacatt
     3541 tcttttcact gatttcagga agtcttttgt tcagtcttgg ggtttacaca ttgtacacag
     3601 tcggggcctc aaatggcggt ataacgatac cgccgattat tttccacaaa ttttttcggc
     3661 ttccaatgcc gcaaggactt ttacttacca atagtcttat cgtctttttg aattattatg
     3721 tttttggatt gttggagaca ctattcgttc tcctatctat tacagtcagt tcattgttta
     3781 tgaaaatact catccgcttg tcccctgtgt caagaaaatt tgaataaagc tggattttgt
     3841 catagcggaa acaaacgata tattaaaaag agtgtgctat aataaaccaa gaaattgaat
     3901 aaagcgaggg atcaatcaat gagcttctct ttagaagaca tccgtcattt actattaaaa
     3961 gaaaatctat taaaagaatt tgtttcaaca agaggctggc atcttgatgt accagaaaaa
     4021 gctgaatttc ttcagttaag ttatgattca agaaaagctg atgcttctac tttgtttttc
     4081 tgtaaaggaa tgaatttcaa agaagaatat ttggattcag caatcgaaca gggtattcaa
     4141 tattatgtat cagaacaacc atacgaaaat tcagcagtag gaatcatcgt aacggatatc
     4201 cgtaaagcaa tggcgcttct tgctatggct ttttacgact atccgcaaaa taaattgaaa
     4261 gtgatcggat tcactggaac aaaaggtaaa acaactgctg cgtactttac aaaagcaatc
     4321 ttggatcaca caacaaataa aaaaacagca ttgctttcta ctatgaatac aacgttagat
     4381 ggcaaaacat atttcaaatc ccatttgaca acgcctgagt cattagattt atatcgtatg
     4441 atgtcagaag cagttgaaaa tggtatgact catctagtga tggaagtttc ttcacaagct
     4501 tacaaaacac aacgcgtata cggcttgact ttagatgtcg gaatcttctt gaatatctcg
     4561 cctgaccata tcagtccgat cgaacatccg acatttgatg actatttcta ttgcaagcga
     4621 caattgatct tgaattcaaa aacagttgtt ttaaatcatg aaagtgacta ttttgattta
     4681 cttagggaaa cagccgatct attccagatt ccaacgatca cttatggtcg ttcggaagaa
     4741 agcaattatc aagtgatccg ttcagataaa ggtactcacg gttttacttt atcttctcat
     4801 gaagatcgct tagctatctg cgatactgct tatgatattt tgctggctgg aggattcaac
     4861 caagaaaatg cagccagtgc gatcattgcc gcagcactaa caggtgcctc cagtcaagat
     4921 gcacaagacg gattaaaaga agctcgtgta ccaggccgaa tggatcaatt gatccaatcc
     4981 aatggagcgc atgtctatgt ggactatgca cataattatc taagcttgaa aacattgctg
     5041 aaatttgcca aaaatgaaca tccagatggc cgtgtaattg ttgtattagg cagcccagga
     5101 aacaaagcaa tctcccgtcg acatgatttt ggaaaagtgc tatcagaaac agctgacatc
     5161 gcctttttga cggcagatga cccagcattt gaagatccga aaaaaatcgc ggaagaaatc
     5221 aatgaagcaa ttactaatcc agatttgatc gttcattatg aaatggatcg tcctgaagcg
     5281 attcgacgcg ctttagcaga aagcgctccg caagattcag tcgttattgc gggaaaaggc
     5341 gtggatccct atcaaaaaat caatggtgta gatgaacctt atgaagggga ttatgcaatc
     5401 gtgaagcggt tgattgaaga ataatggtat ttatatacta gtggtttccc atttctaaga
     5461 aaaaaaggga ctgtgatttt ttgtcacagt tccttttttc ttagaaatca ttttaacttt
     5521 tcactagcta attctgctgc cttttctctt tcttcattta ataatttatt atcatagaat
     5581 ttgataaatg ggaaccagat aatgaatgcg atgattgcac aaatgactgc taacacagca
     5641 gctttccaat caccaccact accaacaaat gctccgatcc cgactggtga aggccacggc
     5701 atctgtgcaa gcatcggttt gacgatttcc agccgaatgg caaaataagc gagtgtagca
     5761 gaagccatcg gagctaagaa aaacggaacc gctaaatagg gattgtagac gattggcatc
     5821 ccgaaaatga ttggttcatt gatattgaat aatcctggta caagtgaggc ttttcctaac
     5881 actttcagct gatcagattt cgcgaggaat gcaataaaga tgatcaatcc taaagtcgca
     5941 cctgatccac caacggttac ataagaattg ttgaattctc ctgccaacgg ataatttgcg
     6001 ccttgtgcgt tagctgccat attagctaat acgatcgggg tcaagaaaga agtgatgata
     6061 ttcgctccat ggatccctac gatccacaat gcatggatca agaaataaat caccatgaca
     6121 ccaagccatg tattcgtcaa ctgcgtaaca aacccaaatg ggatagcaat cattttgaag
     6181 atatccgttc ctagtgcaac taataatcca ttgatgatca tcaccacaaa cgcaatgaca
     6241 aatgttggga tcagagcagt gaaagaacga gaaactcctg gcggaacaac atctggcatt
     6301 tttatgaccc aatttctctt gacacacata caatacaatt cgaccgcaat aatcgacata
     6361 ataatagctg taaagattcc tgaagttcct aaacgttcga caaaagagcc catcctaata
     6421 ccatttacta cagtttctgt ctcattgata gaagcaatca agctgatttt accatcagtc
     6481 atgatcaatt cagggataca cataaagaat gcaaaaacag atagtaatgc ccccgtgagc
     6541 ggattaacat ttaatttttg ttcatctcgt tcaattgacg ttaattcata agcaaacaca
     6601 atggcaaagt aaatagataa aatccccatc gtcattgtat tgacgatcat gtacaagtca
     6661 ctaactttga aaaaagttgc attaaaaaat ccttctaaaa atgggaacgg taatggcaaa
     6721 atatttaaaa ccaaaaacat cgagcctaca atcgtgaaag gaatagaagc catccccgct
     6781 gccatgacag cccgaacgat tttaagttgc gcaattttcc ccataggtcc catcaggtat
     6841 ttctgcaaaa aatcaaacat tagttattct cctttcctaa atataacgct ttttcatatt
     6901 gtttaatacg gatatactgt tggtcttggt tgagtgaaat tttctttaaa cgatactgta
     6961 taaatgcact ggcaaaaata ccgcttacaa caagaatgaa aacaaataac tgtcctccat
     7021 tgttatttgc cataaaagga tacaagaatg taaatcctga ggtaaacaac actggaatca
     7081 ctaataaatt aaagacactc tggaacaaaa aataccactt tgtccatgga acgttcgttt
     7141 ggtgttttcg ataaagcttt acttgttctc ctactgcagg caaaatagct aaaatcagaa
     7201 tcatcggtaa actacttcct aaacttttag ctgatagaag cagaatcatc cattgcatgt
     7261 tcatgaaaaa gaaaaaggca gtcaggtaac gtacgattag ataacgattg aaatacatgt
     7321 ttttcaaact aagttgcttt ttttctaatt cgattttcct ttttactgta tcctgcacaa
     7381 tgatccttcc tttctattta tttttttgat acaaataatt catttcctgt gcaacttcca
     7441 ataatgtcat cgtcgtcatc agatgatctt gtgcatggac catgataatt tccatttcta
     7501 tttttgtccc gctggcatat tctttcagaa gttgagtttg tgactgatgc gcatgaagaa
     7561 tctcttcatt tgcttgtcgc aacttctctt ctacttcttc aaacatctct ttacgcatcg
     7621 agcgaaatgc ttcatgtatc agtgtccgtg catttccgct atggaaaatg atatcaaaag
     7681 aagtcacttg tagtgtttca ctgtttattt tttcagccat ctgttttcct ccttattcga
     7741 tcagtttgat aaatttactg ctgaaattct caaaaatagg ttcagctaag atttcctgct
     7801 gtacttttag ctggtcaact aacctaacga ttgctttagt cacgaccgta attccttcat
     7861 tttcgacata agaaggcgaa actaaaaaaa caaaatgaat ttcttgatat tgatcccact
     7921 gcatcccaga aggaatcaat gcaacggcta cctttgtttc gattccaact ggaacagcgg
     7981 gatgtgggac tgccactgta tcactgaata tgatttgtcc catattttca cgttgctgga
     8041 tctgctttcg catttctctt atatatcctt cttcttcatt tatgctcagc ttttgcagca
     8101 aatcatgaag aatttcttcc tttttcggtt cgtacgtata agtcttgaaa aatttttcag
     8161 acaactgttc ggtgtaaatc tgcttttttt gctgtagaga aaatgctggt atcggtctat
     8221 ctgaggaatg atagggtata gattctccaa ctacctttcg tattttacga acatcatctt
     8281 cattcaaaaa aatactgaca tgtaaaactg gaattttgaa aaacattgtc gaaagatcaa
     8341 tagaagaaat aatcaggtcg attcctttta aagttgtttc attgatctca taatagcctt
     8401 ttacacttac cactgtaata tttttaccga attcactgac tacgcgattc tttaatagtt
     8461 gggcactgcc gtaacccgtt gcacagataa tcaatgcatg tagtttatgc gcagctcttt
     8521 ctttttccaa tgccgccatg agatgcaaag ccaaataggc ccattcatct tcattgatgg
     8581 tatagttttt caaagccggc atgttgctga aatgcctttt tactgcgtca aagactttgg
     8641 gattttcttt ctttatctcc tcagtcaatg ggttctcaat gcgaatacct cggctcaatc
     8701 gaatcatcat cggtgacaaa tgatcgagta gtccattgaa aagctggaga tcctctacta
     8761 atgaaaaatg aaattcctga gacaatcttt tcagaacttt cgctaattcc tttgatagtt
     8821 cctgatcatt ttgattttct ttgtgattcg acttagccat gagatgtaat gtcaaataag
     8881 caatttcttc ttcgggaaac gatacacctg cgattacctc aatacgttga acgatccgct
     8941 gcgccacttg atactctact cgcttagaaa cttcttgttc gatgcctaat tccttgattt
     9001 caaacccttc tcggagccgt ttgatcccca aagccaagtg taaagtcaga ttctgtatga
     9061 taaaatccga aacttttaac ttagcttctc ttgtttcatc gagaatgata atgactagct
     9121 cttcaaaact gatgtctggg aaaaaacggc tttgccctaa atattctttc aatgaatttc
     9181 cataatggtt tccaaaaaaa gtatccatga taaaatgccg tcgatttctt tcttcccctt
     9241 cgatccatgt cccttgacca tgcttagaaa cgatttttag tgaatatgga atcaatcgct
     9301 ccttgatttc ttgcatgtcc tttgtcaaac tggaacgact gatataaagc tcttctgcca
     9361 aatcatccat aaaaagaatg gcatcttcca aaagcaattg gttcaatata tacttttgtc
     9421 gatcttcgga ctcataaaat ccttgcggtt ctctagcata tccaggtact atttcttgcc
     9481 gactcaaaaa caagtcaaat gtcagtcttt gtaaaatatg cagttggtag ccataacctg
     9541 gtttagcaat gatcttacct ccgttttttt ctactaactc tttgatccgc tgcaagtaat
     9601 tacggattgt ccgatcagat aataacaatt cagaagctaa acgttgactg gtgacatact
     9661 ccttcttgtg atcaatcaaa tatctcaaca gcaatttttc cttctgtttc attatcatcc
     9721 actcctattc catcatatgt tctcttcgat cacctctgct aacttctgga tgcctgtagg
     9781 gattgggaca tacgcttgga aagggatact aacaacaggc tttccaacag attctcctaa
     9841 ttttttgaat ttatccaaaa acatcgtcgt ttgcgggctg atcagaaata aatcaaaatc
     9901 actattctta atcatattat cgccttctgt agctgaaact gcatctacga gtacctcttc
     9961 tcttttttgc ttaaagaaat ccgttgtctt tgaggccatc atcgaggatg acattcctgc
    10021 cgcacaaata atcaatgctt ttttcataaa tcaatcactc cttttatttt tataaaccaa
    10081 gcatatcttg taagcgtttt ctattcaaaa tatatatttc cgtagcattg cggaaaaaat
    10141 gtgtatcata tcttgaataa taatttctgg caccgtgata tactagtgat agaatttttt
    10201 caggagggga tttagtgagt atcacagtga atattttgta tacaagtaca aatggacaag
    10261 ccagaaaatt tgcagaagaa atggtcgaaa gaaacattgt caaagcgatt cgtgcagaag
    10321 aaggaaacga gaaatatgac tattactttc cactagaaga tccagaatct cttttactga
    10381 tcgaccgttg gaaagatgaa gaagcaatcg aaaagcatca caaatccgaa atgatggctc
    10441 aaataaacga actccgtaaa aagtataaat taaaaatgaa agtcgaacgt ttcactgatt
    10501 taccgtaaaa agatacacat ggaatgatag caatttatca tccatgtgta tcttcacttt
    10561 ttccttattt tttattcgat ctcatctaat gattcaaacc gtgtttttct cttatttttc
    10621 ggataagtat ttcagtcacc ccaatataca tgattcctaa acatacccca aggatattct
    10681 caaaaatgcg gatcgttact gctcctggaa ccccgaataa actggcagtg attgccaaag
    10741 caccaaaaga attaaaaact gttttatatc tataggtaga acaaacacct aaagctagac
    10801 cacctaatat accgacccag ttaaaaggaa taaattggct gataccaata aataaaacac
    10861 atccaatcaa tgagccaatg attcggtcta ctgctcgttc tttgaaacca atcgacatca
    10921 atccatagct agatacaata gaagaaaatg caaaccctgc ccacatcaat cgctgaaaag
    10981 ggacatactc tcctgcaacg atcaaaagac taatgccaaa tgcatagatg agcatccaaa
    11041 ttttttcctg cgaatacatg cctttcaaaa aaatctcttt aaacaagctt ttgtctttgt
    11101 tcttttctcg atgttttctg taaaggagaa ctgaaaacca acaaaaaaac aaaaataata
    11161 agctacttgt ttgtgtaaag aaattcttgt tcaacgaatc tttaggtaac gaataaacaa
    11221 taaagagata tgaaaagccg tacaaagaag cattccccat tttgggatca ctagcggttg
    11281 ccattaatat gctgcttagt aaaataaaat gaatcaacat gcccataact gaccatttga
    11341 tcagctggac atacggtgct actaatagtg agaacatgac gattgctaaa cctatgatcg
    11401 aatgggaaat cttataacca aagtcaacaa aacgaatact catcaacata cagaataaaa
    11461 ccacagcgta tggtttatgc ttcgattcaa ataatgttgt aattataatg ataaaacaaa
    11521 tggcaaacaa tacgatgaat aatgatcgta aaaacaatga acaaatatac atgcactttt
    11581 cttttttaga agctgcttct cgtatttttt gttttaaaat aaacggatct agttgtagag
    11641 actgataaaa atccacctgt tgtcgtcctt tctttttact tttatttttt acagtcatct
    11701 atgacaggtt gtaaatacta tggaacatca tatggcgttg tcattttctt gtcaactatt
    11761 tttcctattc atgtttttat aaaaaaacat atcgttttct tgtatgatta aatcaccaca
    11821 aaataaacaa ctgagagaac gatatgcttt atgaatggtt ctatcaaaaa aattctgaga
    11881 tcaatagaca aaaatttgat gattacagag gtctcttacg aaaccttaca gaagaaaaaa
    11941 acattgatcg ttgctgcagt tctcttccct gctccatgtg tctgtagaaa ttgtggttct
    12001 gctgtggtag atggaaatag gaaattaatg gtggtgaaaa acaagaagaa ggaaaccatt
    12061 gtccactttg aataatataa tcatatgcca atggttatac gcctaaaaaa gcagcgctat
    12121 acctgtaaaa actgtcgaag ccattggaac gctcaaagtt attttatccg tccaaggcac
    12181 tcgatttcaa atcatgtcag acataaaatc acttctttac tgactgaaaa agtgtcgtta
    12241 ttttttattt cgaaaagttg ttaggtatct ttgacaaccg ttattcgcac attgaaagag
    12301 ttgagaagct atttaccaaa gtcttccaaa agaattctgt cttgagtgtt gagggttgtg
    12361 aatttcgttc tcacgtttcc gttgaggata aactgagttt tatttccgcg gatggcgaga
    12421 cagaaaaatt aatcgagaaa atttacctac acaaaaaatt cctcgattaa caaactattt
    12481 ccttagctgc actaatccta aagaaataga attcttagtt acagacatga acgccaccta
    12541 ctttcagctc accaaacatg ttctaccaaa tgcgaaattg gtgattgatc gattacttgg
    12601 tttttccacg cctttcaagg aggcgtaacc tttattcacg agttggttga agctttccga
    12661 gacaaaagac ccgacttgtt tttctcctta ttgacaaaga gccattaaaa ctagagaaaa
    12721 aattacaact gatcgctgat tatttgtaga aagaaaatca aagtattacc caataaactt
    12781 gagtcattag gcaaaaaaca ctgcgaaact caagaatgat tttcgcagtg tttttgtata
    12841 ttttttatta actagtatta ctacaaaggt ttatttttca cttctcaacg cagcacaaaa
    12901 ttgtctttac ttatttactg gttgggttca accttctcta ctccgatact aatatttgtt
    12961 tgtatacccc ttctaaaatc ggtagtttcc attcaattga tgggtactag ttgattttaa
    13021 aaactatgct tattcaattt tttattttag ctcatatatt tcgtttcttt ctaaatttta
    13081 tactgatgaa aacaagtagc acacccaata ttgacaatat aaactggcta atgatggttt
    13141 ctccagtttt tggaagatgc ttatttccac tgcttgttgg attgtttcct cttatatctg
    13201 agctatcagc aacttttcta ttctcttttg ttaaaaagat cgccacttct tgttcttttt
    13261 cgatcttgaa agttactggt gtcgcatcca attcataccc cgttggcgct tgagtttcta
    13321 ccagctgata tgctcctggt gctaagccat ctatcgccag ttttccatct gctcctgtgg
    13381 ttagacctga ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca gctccttgca
    13441 ggacttctcc gctttggtca tcgatctttg tcaacacgac accgcctggt gtaagtcgat
    13501 tttctttcgt caattctacc accgctgtct gacttctttc gatctcaaac tcgattggtg
    13561 tcgcatccaa ttcatacccc gttggcgctt gggtttctac cagctgatac actcctggtg
    13621 ctaagccgtc aatcgccagt tttccatctg ctcctgtggt tagacctgac tgcagaacgg
    13681 ttccattggc atcttgtaac tcaaaaacag ctccttgcag gacttccccg ctttggtcat
    13741 cgatctttgt caacacgaca ccgcctggtg taagtcggtt tgctttcgtc aattctacca
    13801 ccgctgtctg acttctttcg atctcaaact cgatcggtgt cgcatctaa
//
LOCUS       DAZKMK010000074        13365 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000074 DAZKMK010000000
VERSION     DAZKMK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.075"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(545..1159)
                     /locus_tag="ACG8V1_002084"
     CDS             complement(545..1159)
                     /locus_tag="ACG8V1_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0330170.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1765..2142)
                     /locus_tag="ACG8V1_002085"
     CDS             complement(1765..2142)
                     /locus_tag="ACG8V1_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330171.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2442..3323)
                     /gene="galU"
                     /locus_tag="ACG8V1_002086"
     CDS             complement(2442..3323)
                     /gene="galU"
                     /locus_tag="ACG8V1_002086"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0330172.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDVVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3366..4385)
                     /locus_tag="ACG8V1_002087"
     CDS             complement(3366..4385)
                     /locus_tag="ACG8V1_002087"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0330173.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(5018..6022)
                     /locus_tag="ACG8V1_002088"
     CDS             complement(5018..6022)
                     /locus_tag="ACG8V1_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0330174.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(6035..7084)
                     /locus_tag="ACG8V1_002089"
     CDS             complement(6035..7084)
                     /locus_tag="ACG8V1_002089"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0330175.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(7101..7922)
                     /locus_tag="ACG8V1_002090"
     CDS             complement(7101..7922)
                     /locus_tag="ACG8V1_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0330176.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7909..8688)
                     /locus_tag="ACG8V1_002091"
     CDS             complement(7909..8688)
                     /locus_tag="ACG8V1_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0330177.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8705..9175)
                     /locus_tag="ACG8V1_002092"
     CDS             complement(8705..9175)
                     /locus_tag="ACG8V1_002092"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0330178.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9187..9606)
                     /locus_tag="ACG8V1_002093"
     CDS             complement(9187..9606)
                     /locus_tag="ACG8V1_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0330179.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9687..12464)
                     /locus_tag="ACG8V1_002094"
     CDS             complement(9687..12464)
                     /locus_tag="ACG8V1_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0330180.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12655..12879
                     /locus_tag="ACG8V1_002095"
     CDS             12655..12879
                     /locus_tag="ACG8V1_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330181.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3940 a         2776 c         2157 g         4492 t
ORIGIN      
        1 agaaaatctc gtcctgtttt tttcatttta caatcattga aaaattgaat ttcattgcaa
       61 aaaaaataat aaacacttat aaatatccat tatctatcct tcaatttttg tgtattttat
      121 ttctattaga aaaaggtatc ctgttggaca attttgttat atcaggtaaa cttcattgca
      181 ataatgataa caaatgtact ataattactt tgtaaagagt tttggggaag actctttcat
      241 caacgtgtat taagagacaa gaaagaatcc atatggattc tttcttgtct ctttttccac
      301 tactttttat ttttctgaca ctacatattc ttataactca tttctcataa ctgtgaaaca
      361 acttataaaa ataacaaact ttaaacaaaa caagcttaaa atcaaagatg atccatctta
      421 aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg ttttcttttt
      481 ttcaaacgct ccatttttat gtggaacatt atttttttaa ggaactttgt caacatgtgg
      541 ataactattt tccacttgtg tcctggacta gctgttttgc tgtaaaatac aaaacttgct
      601 gcagcaaaaa gatcgttgca agagaggctg ttccgcagat cgctggatct agatttggca
      661 aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa
      721 ataatcgcaa accactgcta tgttgcgatt tgatacttcg tgacaaatca aatccagtaa
      781 caatgattaa tggatgacca ttttctttga tccactgagc tagttcgatg attgccgggt
      841 ttcttccttc attcgaaaga agaagaaaaa tgtcttctga tttgacagaa agctgatcaa
      901 taattgcctg tcctgcacct tctagcgttt catagacacc tccggctgga ttttcaatca
      961 atatacttgg tacaagatcc ggggtttgat cccaaaattc aaatgctaca ccacttagtg
     1021 tgcggctagc atatatctgc aatctaccac cttgattaat ttgtttcgcg atccaagtgc
     1081 cagcttgaag catcagcggc tcttctatcc ttaatttctc ttccaattgg tgataatagt
     1141 caaaataaca taaactcatt tatctctccc gcctagctat acttgacatg aatagaaaca
     1201 gctgcctaat ctaaggaagt acataaaaaa acagtagtta gacagtaaca catgtggtct
     1261 ttgctgtttc aaacacaaaa aaggcattga acgaatacgc ccaatacctt gacatagcaa
     1321 ataaaacaac gtgcagacca tggaaaatat ctacataggg gtagaatttt caccctagat
     1381 taggtacaat aaattaggtc gatgcactgc attgactatg gcaaaatgat cggtcagggt
     1441 gtatgtgtgt gactggtcat tccctgattc ttagcgccaa ctaagggtca gggagccttt
     1501 ttttattcag ctgtttctat ctgaatttat tgtaagtaat ctcttatcca agtgcaagca
     1561 agaaaataca aaaaaacatt ggtatgattt atatatatat aaataacata aaaaaaggaa
     1621 tggcttatcc cgatacaaca agcattcctt taaaaaagat tcttttatat aagtgaaaaa
     1681 agcttttttc caccaattaa ataaatctct catctcaata gtttatccat tattttatct
     1741 ttttttgcgt atacctacta aatattaccg tcttcttctc gttcttctac tttgttcgag
     1801 agaatctgta ctagaaaaag tagttgtatg tctacctttt ctgcctatcc gagactttct
     1861 tttcatatat gggatataaa tgattagata cagtacagca aaaaataaag caaatacgca
     1921 agcttcccaa ctacctaacg ccaattcaac catcaaggta gcaaccccaa cggatgctgc
     1981 ataccagata aaaatcagaa acgtttcttt ttttctgacc atgcgaaaca tgataaatag
     2041 actaataagc aatagtggca acacaaatat ccaattgctt ccttccatca gccacttgag
     2101 tatatcaagc agcgactgtg caacagcgac taattttccc atagtgaaca cacacctttt
     2161 tttataatta aaaaattcgc ctttttattc tactatgaat cttctggctt ggctttcatt
     2221 tctcttatgt aatcattttc ttttatacaa cttaaaaaaa aattaaacca ttagcataat
     2281 attttctgaa tctattcacg aaagtctttt tctatctttc gttttccacg actcaactga
     2341 tctagagtca aaaaactaaa acgacagttg cgcaaatgac gcacaagctg ccgctttaat
     2401 aacaaatagc ctttcattct cagcaggcca cttatttcca gttaactttc tgttcttagc
     2461 tttttactca actggataat atatgcactt aatgaagtcc caatttccga atgttgaagt
     2521 ccatattcaa tatttgtctt catatatcct aacttatcgc ctacgtcgta tctttttcct
     2581 ttaaactctc tcgcatagac cgcctctgtc tggttaagag tctgaagtgc atccgttagc
     2641 tgtatctcgt ttcctgctcc tggttcttga gtttctaata tctcaaatat tttaggagtc
     2701 agtagataac gtccgataat ggctaaatta cttggtgctt ttcccatttc aggtttttct
     2761 acaaaccctt ttactctgca acgacccttt tcgctatctg ttttaggatc aataattccg
     2821 tatttagatg tttcattttc tggtacacgc atgacggcaa cagcggaagc ttgtgtttct
     2881 tcataatcca tcatcaattg ttgagtcaat gggatatcat ctttcatcaa atcatccccc
     2941 agcatgacga caaacggttc atttcctata aatgcttttg cttgaagcac gacatccccc
     3001 agtcctcttg gatgagattg acggatgaag tgaagattga cttgagtcgt ttcttcaact
     3061 aaagcgagta attctgtttt ttccttttcc cgcaaattgt tttccaattc gatatttgca
     3121 tcaaagtgat cttcaatggg tcttttttct tttcccgtta cgatcaagat gtcttcgatt
     3181 cccgaatcca atgcttcttc cacaataaac tggatcgttg gtttatccac gattggaagc
     3241 atttcttttg ccatggcttt tgttgccggc aaaaaacgtg ttcctagccc agcggcagga
     3301 atgactgctt ttttgacctt cacttgctgt tcctcctaaa cgacgattaa ctattgatca
     3361 tctatttaac gaagagaaaa ttcattctcc atttttccgt cacgtaacat gatttctttt
     3421 gctgcttttt tgatatcttc tccattatac aaaacattat agattgtttg agtaattggc
     3481 atctcaacac ctagctgttg agctagctct actgctgctt ttgtcgttgc cacaccctca
     3541 acgaccattc ccatgttttc tagaacttct tccagcttat gtccctgtcc taacaagtta
     3601 ccggcacgcc aattgcgaga atgaacactt gtacaagtaa cgatcaagtc ccccacacca
     3661 ctcaaaccga taaatgtcag aggattggca cccatcgcga caccaagacg gctgatttct
     3721 gccaatcctc gagtcatgat tgctgctttt gcatcatctc cgaatcctaa tccatggata
     3781 gcgccggcac caattgcaat gatatttttc agtgctgcac cagtttctac gccaatcaca
     3841 tcattatttg tataaatcct gaaataatca ttcatgaaca gtttttgtac ataggctgct
     3901 gctttttgat caatacttgc agctgtaatt gttgtgatat catgtactgc cacttcctca
     3961 gcatggctag gaccagataa tacaacaatt gcctgtcttt tttcttctgg aatttcttca
     4021 gccaaaactt ctgagattcg tttatgcgta tcttgttcta gtcctttact tgcatgtata
     4081 atgattggct tagtattcat tttaccaata agttcctgtg cgactgaacg aatcgctttt
     4141 gttggaacaa caaataaaat agcatctgcg ttatccactg cttctgccaa ttctttatat
     4201 ccgataatcg attctgggat cgtcaaatca ggtaaaaaat gctgatttgt atgatactta
     4261 ttgatttcat caatctgttc aggtacattt ccccaaatac ggacatcatg cccgttttct
     4321 gccaatgttt gtgctaaagc agtgccccaa gatccaggac caagaactgc gattttttgt
     4381 ttcatggata ttcctccaaa taaataattg ataatttcca ttatagtata catttcttaa
     4441 agttgggact aatttccatt gtttttaagt gtttgtttac ttcttttatc gtttttactt
     4501 gaaataatca atgatatgtt acaatagtct acattttatt tttacactcg ttatgctttg
     4561 cttgttccta agaggaagca gttttgacaa tgttactacc agcattacta ttaggaggtt
     4621 aggaaacttc tcctagtgag tattgattta aaatacataa gagacagatt gaacatttct
     4681 aaatctgtca ggtcctgcaa cggacagatt gtctctgttg acggttgaca taggaacttt
     4741 tgttttagtc aacattctgg atacaaaacg ctatgtaaac tggcgcaact cgttgtactt
     4801 ggacttttac tacggatcca tgcaacaact aaatagtact attgcataac aaaaaatggt
     4861 tggcacccac atgtcccatt aaacagatta taagttgaac tgtagataat gcaaaaaatg
     4921 tgttttataa cgtaattgat gccaatattt taaaaaaata aagggagcag ttatcgctgc
     4981 tctctttatt ttttatattc tttttatcca gtctatccta ataatcaaac tgacgatagt
     5041 agcggcgtat ctctaatgga tggcagttca actgctccac atgagcatcg atgcgttgtg
     5101 tcactacatg catcaataag aaagacaaaa cacgtccgcg atatttttca ctgatacctt
     5161 ctactggata gtcttttgtg tctatcaatg tataattgct gcaaattttt ggcaaaagtt
     5221 tagccacacg ttcagataac actctctgtt catcttcgcc taaaaataat gtgactgggg
     5281 ttgtttcttc tacgatctct aacgtcccgt gaagaaactc ggcagcatgg atagatttcg
     5341 atggaagcca gctttgttct tcccagtaac acatggcata tgaataaaca gctccccatt
     5401 gattccctgc tccaataaaa taatgcatac tatcacgtcg atgtttttct gcaaatgata
     5461 agccaaatgc atctgctttt ttctctgctt ccactaaccc tgttggtaaa taacgttcga
     5521 gctgttcata gtattctgaa taagcttcga attctccatt caaatacatc aatcgatctg
     5581 ctgccataaa aaatttgatt tgttctgttc caggagctgg ataagtgacc acaaaatcgc
     5641 atagctgaga caacttactg ttcgctttat cgataaatcc aatcagtgtc gctcctactt
     5701 tcttgatttg ttctactgct ttcactactt cttgagttgt accggtaaca gaggacaaaa
     5761 taacgataga atcttttgtt aatcttttgt ttccagtagt gtaatattct gcagcatgct
     5821 gtacaaagac aggcaagttg cttttcccat tcatataagt gacagcttgc atcgaggaag
     5881 catacgttcc accgattcct agataataga cagcgtcaaa tttttccgca taaagtttat
     5941 cgatgatttc ttctacttgc ggacgtaaag ctagtgcgcc ttttattgct ttgatttgtt
     6001 cttcttcatt gaatttcagc atgatttcca cctcttataa tttacttttc aagactgtat
     6061 caaagtcttc aaaaatcttt ttgttcaagt caataccttt agctactgcg gttttataag
     6121 cgaatagttg aaacggaacg acaagcgcta atacagacag atattcatca cattctattg
     6181 ctaatccaag tgttttctcg tttcttgctt tttttgtggt aatggtgaga gtctctccaa
     6241 cgtaccgact catatatctt tgcaactttt gcgaacgttc cttatttaca ctttcccctt
     6301 caataaagaa tagcaaatgc gaagcatctg cctccaggta tgggccgtgc atataagctt
     6361 ctaactcaaa tccttgtgac ggaacgcgga ttgtttcagt caatttcgtc tctgcttctt
     6421 tagctgttcc ccagtttgga ccatatccaa tggtgacgaa acgctgtgct aaacgaaata
     6481 aactttgata tccatcaaaa aattcttccg ttttttgaat aatcgctggt aaatggttga
     6541 ttattttgcg caactgttgc atgtagtctt gttctatttt tttgctgatt ttatttttac
     6601 ttattccaat tcctatccct aagagtagta actgtaaaac agtcgcagaa tagccttttg
     6661 taacgaagcc tactgattct accccggcac ctaaatcaat aatttgtgca gcttctaaag
     6721 caagtggact ttgcacgtca tttgtgatag caacaacagg taacccttgt ttttgtatca
     6781 tttttactgc ttcaacagta gaagcacttt tcccgctttg tgacacagca atcacagtat
     6841 cgattgaagg agaaagtttt ccataattga aaaaataaaa aggttcttga atatcgacag
     6901 taagatcacc ataagattcc aatgctggct tcgctgctaa acaagcgtta taagatgaac
     6961 ccgttgctaa gatcagtaaa ttcactgttt ttttcatatg ttctcttgtc gaaaaatcat
     7021 tctttttcaa gatcgtactt agtacttcct tttcttcata gatataatct tgcatcgtaa
     7081 acattttctc tgctcctttt ttaaaaaatc cctatccacg aaccaaaaat tccaaagaaa
     7141 gctagtccga tcaagatcgt taatggtttc acttctttct tcaatagata aaacactatg
     7201 ccaaatgctc caagacataa gatatttgga acgatatcat caaaaatact ttgaacagtc
     7261 acagcatttt ctcctgaacc gatttttaaa ggaatattga tggtgatcat cgtggcagtc
     7321 attgctccaa cgaccatcag tccaataata gacgcgccta atgtcaaact ttccatcata
     7381 ccgtttgcct ggatcttttt caaaaaaccc gttcctaagc gatatcccca acctgttgct
     7441 agatagcgaa acagaatgtg cggaatattg aaaatcaaaa tgaacaagat agggcctaaa
     7501 atatttcctt gcatggctaa agaggtaccc acacctgtgg caatcaatcg caaagttccc
     7561 caaaagaaag aatctcctaa ccctgcaaga ggtcccatca aagatgtttt gatactatcg
     7621 atcgcagtca catcaaattc aggatcatcc ttattttctt cctccattgc agccgtgatt
     7681 cctaaaatca gtgtaacgat atgtggggtc gtgttaaaaa actctagatg ccgtttcaaa
     7741 gctgctgcca tttcttcttt cgactgatat aactttttca aaatcggaat cattgcataa
     7801 acataagcca tattcatctg acgttcataa ttccaagaaa actccatttg aaaagaacgc
     7861 caaaatacgt gatttagctc ttttttcgtt attttgctgt ttttgatctc agaattcttc
     7921 atcttcttct acctcccctg cagttgtttg aagtggtagt tgacggacat tcataatatt
     7981 gactaccaca actgctaaaa tcgcaccaaa aatagctact ccggtcacag gaatctctaa
     8041 gtaagaagct atagcaaaac ctaagaaaaa atatggtgca acttgtttgt tgatcagcaa
     8101 acgagcaagc atcgcaaaac caagagctgg aatcaaccct gtagctactg acaacccctt
     8161 ttgaacgaag tcaggaatca tatttaatag atttccgatc gtattgcttc ccaccatata
     8221 agaaaccatt accacaacac caagcatcaa ggaaagaccg ataccggcaa gcaagtgcat
     8281 atgctcgact cctttataat ttccttcttc tgcataattg tctgctttat ggttcataat
     8341 tggaatcaaa agccctaagt aaatattttt caatatcaat gtcaacgtag caatcggcaa
     8401 acctagtaaa agcgcggttt ctgtaccggc tccagctgta atagcaaaag ccgtccctaa
     8461 aatcccaccg gtcacaacat caggtggaat cgaagcacct actgagaacg atccgataaa
     8521 tgctaactct aaagttgcgc ccataatgat cccagttttt acatcaccta acacgattcc
     8581 tgtaaacagc cccgtcacaa tcggacgaga aagcaacgat gtccctagtg catattctga
     8641 ttgtgcgaca aatgcaacaa ttcctaataa gactgcttgt acaatcattg aggttcttcc
     8701 tttcttacaa aacttctctt acatccactt ttttatctgc tggtacttga cggatttcta
     8761 cttctatttg tttgtcaatt aatcgtttca agcgttcctc ctcttctggt aaaatattta
     8821 ccgcttttcc gatgctgcga gtaccaggtt ttgccttgat ccctcctaag ttgatggttt
     8881 tgatttctgg atacgcttct gccagcttat atgcatcctc gatcgattcc actacgatga
     8941 ataacttata tttatctgtc accccgcttt tcaacgcggc aatcgagtca tcaattgatt
     9001 tgatcactag tttgactcct tgtggcttgg ctaatttgat cgttgtcttt cgtatctcat
     9061 tcgtaggtac atcatcatta gcaatcaaaa tacagtctgc gcctattgtt tgtgtccatg
     9121 aaaaagctac ttgtccgtgt aataaccgat ggtcgactct tgtcaataca atcatttttc
     9181 ttcctcctaa aattcgtctt cttctatttt ctgattgagc cgctcgttac aaaactggat
     9241 catttttttt gaatcagtta gcacctgctg gatcaaaaca gttgtctcta cttcagcgtc
     9301 caaccgagat actagctcaa tcaatagtgg caaattcaat ccggcaacga gatagacatg
     9361 atcccgattg atatattgta agaattcgtt gttgacactt ccgccaaaaa gatccgtcag
     9421 aacaatgatt tctttatctt tgtacctcat aaaaagctgc cttactgttt ccgctaaatc
     9481 aaaattctca tcagtatagc aatcgaatac agtaatagaa tgatttttcc catagatcag
     9541 ctctaatgaa tctaatatcc ctgaagcaaa ttttccatgg gaagctaaga taatttctct
     9601 atccactttt tcatctcctc ttttcactta cacatactaa aaaagcaagt tgcatgccaa
     9661 aattagtggc aacaacttgc ttatgttcaa aattcttctt ctatcgagag aaactctgta
     9721 tcttcactca aaatatcata gatataggca atctcagaat tggggatcat gacactataa
     9781 tgctcttcaa tgacactaaa cgcagattta atagcagata acccttcttt ttgacactgc
     9841 ttccatttct ctaatccttg ataggtttcg attggcattt gtcggatcaa tcgttcaatg
     9901 agacagctca catggacata caacgctaat tttttgggat tgctgatgct tcgattcaac
     9961 tgcacctcta attctctcat aaaatgatcg atttctccca tcaccttctc tgtatctaga
    10021 atagtcacgg aatccagtac tttttccaat gaaaaattac gaatgatgcg ctgattgaat
    10081 atttccttct ctgaagcatt catagtagtg tccaaccatt cagctagtac agacatcttt
    10141 tcccccgaga tgaggtcttc taatgaaaga tagtcgatat tggtgatatc aggatctgtg
    10201 gtgccaatca ttcctacgac ttcgtatatg gaaaataatg ggtcactgtt tttcttatct
    10261 tttaattgct gatattcata tgggatgatt ttcaactgac aagccgtagg taagcttttt
    10321 tctagcagtt cacttaagtg gaccgccgta ccgattccag tgaagcaagt agtaatgagt
    10381 gctttgggtt tgttttcctc tggatagagc agtcgccatt ctggctggat ctgtcgaaca
    10441 gtctcttctg ctatctcttc taatgataca tttttttgca ggcattcccc tactgtgatt
    10501 gctaaaggtg tcgtcacatt attcaaaatc aaaagcggta cggataactg ttttttaaaa
    10561 aactgatgga tttctttcag agatcccatg tctactaata tgatcaatcc atttgaaaca
    10621 tcgctgtttt cgcaataatc aaggatttct gaagcaatct gctgtggtgt gacatctagt
    10681 ggcatatcaa atgattcaaa tagatcctct tgtaataaac gatttactac atttgccata
    10741 ctgctagcag ttgcataacc gtgagcagca atcatcgctt ttggcagtcg tttttctttt
    10801 gaccatttcg ctttttttag atagatcgtc agtaaaatcc gatccatact tgtgtcttca
    10861 atatctaatt tgatcttact taattccaga attcgttgga catagtgata actagatgga
    10921 taagactgag aaacttgttg ttctaattct ttgattagtt ggataatatc atgttcttcc
    10981 ggtaaccatt tgatagtgct tcgctgatag agatagtaac tcaaggcata gacactgttt
    11041 ccatcgaatt tgatttgata ggcagtttcc atttgtcgaa aggtctctcg gatagattgt
    11101 gtccaataaa ccagcagctc gtgtttttct tgtctactgg tctcaaacag caaaaaatca
    11161 aataattgaa tcacttcttg ctttaatttt ccttcgcaag aatatagcag acagttattt
    11221 ttcttgaaat caattaggat ttgctcaaaa cttttaacga ttcgctgctg ttcgggctgt
    11281 tgcttctcca ttaattgctc taaggtctgg tcttgcgtaa tatagattcc ttgctgtgct
    11341 gtatttttag taataggctg cgcaagtagt tctttttgca aatgatggat cgtgacatgg
    11401 atttcaggct gttcacgctg ttcagcatat gcttttgctg ctgaaacttt gacgatattt
    11461 ttcagctctc ctatgtttcc ggataatttc tgacttgtta aaagtgacaa agctggtcca
    11521 ctgactgaca aagggctccc catttttctt tgttcttcga gaaaaaaaga gtaaatcagt
    11581 tctagccgtt cattacgact acgctgatta agtgcaggta gttcgattcg gataggtatt
    11641 cttcgaatga aagttgttaa gaaatgacta gtaatctctt cagtcgtagc aaaaaagaga
    11701 cgtgttttga tccggcgtga caaattaggt tctcccatac gataaatcac accttgatca
    11761 agataggtga acagtttttc ctgaccttct ggtgttaagc gatgtacctc gtctaaaaac
    11821 aagatcccac catctgctgc ttcaaaagcc ccttttttat tttcttctgc accagtgtac
    11881 gctcccttaa catgtccaaa caaattgcta gtcaaaagtt caggattatc tgcatattgt
    11941 gcacaattaa aagtgatgaa tggtgcagaa tcttctagca gatcatgaag cagacaatac
    12001 tgatagacca aattaactaa ataactttta cctgtgccac tttcgcctgt aatcaaaagt
    12061 ggtaatccgc catctggata gtaaagagct gttttgattt gttcgatact ttctttcaaa
    12121 ctggcgtcgt atccgatcat aaaagagaat aagtcttttg gttgcgcgaa aaaaggctgt
    12181 tcctctttga gatcatcgat agaaaaataa aaattcgtgc gtaacttaaa aaattgtttt
    12241 tcaaaagctt ctttgtggaa ataaatcact ggtcgagaat tgattttgac tagtagttgc
    12301 tcatcattta actggttcaa ataatgactg actgtgtttc tttttacccc aaaatgatca
    12361 gctaaccttt gtgctgtaaa gacctcgttc atatgttcta aatcgaaaaa ggctgtctgg
    12421 ttctctaagt aacataaaat ttccttcttc aacaacttat tcacccctcc tccatcatag
    12481 aaaacggatt cattttgtca ataaaatctt taataaagaa ttgaaaaata tatcttatta
    12541 tagacagcag cctacttatc ccttataatt tttattctaa aaaacgtaca gttccttcca
    12601 gtttttatac tggaatttca acatatgaaa gaataaggag gctatgacta ttcaatgaaa
    12661 tcggataaat ataaaaacag gaaacatact tcctggaaaa cttttcgaga aatcatcgga
    12721 tcttatctcg ctgtagttgt atttttgcct ttgctttata tggctatgac agaacaaact
    12781 cgttttattg gcgtagatac gcaatatata gccgaaacag gatttttatt catctttatc
    12841 ttgatttttg cgttctataa atccttccat acaaaataag attcacagat ttcaactttc
    12901 ctgtctgttt ttagctatta caaatcctta tatgtggtat aacaacaatt tattggcata
    12961 atgaggaaaa acgagttatg atgctttcag agagtaaaga ggtactgttg tcaccatcct
    13021 ttttattgat tacgtacacc actcttagtt ttttcccatt gattacgaaa acaaggtaga
    13081 aacattgatg ttcctacctt gttttctttt tttattttca gatcaaaatc tcaagtagct
    13141 tttgacttga tgtcgggagg ttgtcttcgt tccatcaaac ctgatagaat ctgcgccaaa
    13201 ccatagtttt ctccactatt atttattcct ctaagatagt acccacaata aacgaataaa
    13261 tatttttctc attttcatac tccttctttg aaaatcaatt agatttctct caacccactt
    13321 ttttacaaaa attacgtagc aaaaaagatt gcaagaacaa attaa
//
LOCUS       DAZKMK010000075        13294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000075 DAZKMK010000000
VERSION     DAZKMK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.076"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8V1_002096"
     CDS             complement(105..629)
                     /locus_tag="ACG8V1_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0330182.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8V1_002097"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8V1_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0330183.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8V1_002098"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8V1_002098"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0330184.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8V1_002099"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8V1_002099"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0330185.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8V1_002100"
     CDS             3157..4026
                     /locus_tag="ACG8V1_002100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0330186.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8V1_002101"
     CDS             4052..4567
                     /locus_tag="ACG8V1_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0330187.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8V1_002102"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8V1_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0330188.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8V1_002103"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8V1_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330189.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8V1_002104"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8V1_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330190.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8V1_002105"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8V1_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0330191.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8V1_002106"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8V1_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0330192.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..9090)
                     /locus_tag="ACG8V1_002107"
     CDS             complement(7873..9090)
                     /locus_tag="ACG8V1_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0330193.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9348..10625)
                     /locus_tag="ACG8V1_002108"
     CDS             complement(9348..10625)
                     /locus_tag="ACG8V1_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330194.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8V1_002109"
     CDS             complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8V1_002109"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0330195.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11547..12491)
                     /locus_tag="ACG8V1_002110"
     CDS             complement(11547..12491)
                     /locus_tag="ACG8V1_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0330196.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12493..13179)
                     /locus_tag="ACG8V1_002111"
     CDS             complement(12493..13179)
                     /locus_tag="ACG8V1_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0330197.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3776 a         2774 c         2397 g         4347 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt gatcatttgt gtcgccagtg cttttttttg
     8341 ttcgagtaaa tcttggagaa gttcggcgta atagatcgtt ttttgacagt ggatactgtc
     8401 tttttcaaca gctggatagg cacactgtgc atagtggatg atttgatctg ccttttgctc
     8461 ggcacgattc gcggagattt ttttccgtgt ttcgttgatc agtttatttt ttatttttgt
     8521 ccgagtagat gccaatacaa aatctggatg gggaaaaaga cggatcaatg tcaatgcgta
     8581 aggggtcagc ctgtttgaaa agaactgttc aagctctgga aaagtcaatt gaaggcagtt
     8641 gtgtaaatcc atgcgcagac gagtgatttt cttctcaatt tcttgataaa aacgggctaa
     8701 atcgcgcatc tctaagtaaa aattgctttg tacttctttt ggctgacgtt gatttttaag
     8761 atgagtctgt gcgagccggt gggcatccga tttatctgtt ttccaactgc gtaaggtgct
     8821 ttcttccatc tgttttttcg cctctaacgg gtttaacaaa cagtaagaaa agtgatgatc
     8881 ttgaaagaac ttttcgactg gtctggaata aatccccgtt gcttcaaaaa caatcaatgg
     8941 acgctcaggc aagtgctgga tctcttcgag taatgcatga aatcctcttt gagtgtgttc
     9001 aatctcgtct tcaaataagc aggtgtcgtc tttgtatagg acactgtagc tgtgtcccat
     9061 tgaaacatct aatgcaagaa tgtaaggcat ttgttttcct cctttttaag ttttgaaaga
     9121 actttactgc ttcatttcga ttccaatttc ctatacacgg gctcatggcc ccaacatacg
     9181 taaacctgat tctaaaatga cagtaaagaa gttcggtttt tgatagggac tcgtagtcct
     9241 caaggctttg ttcgaacttt ctttcactct tcactataat ctaaaaatag aaaatagagg
     9301 agaacatcat tcgtcaatga tgttcccctc taatcttagt atgtttttta tagtaattcc
     9361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
     9421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
     9481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
     9541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
     9601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
     9661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
     9721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
     9781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
     9841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
     9901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
     9961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    10021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    10081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    10141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    10201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    10261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    10321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    10381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    10441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    10501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    10561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    10621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    10681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    10741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    10801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    10861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    10921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    10981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    11041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    11101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    11161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    11221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    11281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    11341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    11401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    11461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    11521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    11581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    11641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    11701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    11761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    11821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    11881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    11941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    12001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    12061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    12121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    12181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    12241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    12301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    12361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    12421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    12481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    12541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    12601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    12661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    12721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    12781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    12841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    12901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    12961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    13021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    13081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    13141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga
    13201 caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat
    13261 agacactatt tttaaatatt tttcgttatt tttt
//
LOCUS       DAZKMK010000076        13195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000076 DAZKMK010000000
VERSION     DAZKMK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.078"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..1901
                     /gene="yfcC"
                     /locus_tag="ACG8V1_002112"
     CDS             162..1901
                     /gene="yfcC"
                     /locus_tag="ACG8V1_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0330198.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2058..3242
                     /gene="iadA"
                     /locus_tag="ACG8V1_002113"
     CDS             2058..3242
                     /gene="iadA"
                     /locus_tag="ACG8V1_002113"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0330199.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3353..3556
                     /locus_tag="ACG8V1_002114"
     CDS             3353..3556
                     /locus_tag="ACG8V1_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0330200.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3657..4319)
                     /locus_tag="ACG8V1_002115"
     CDS             complement(3657..4319)
                     /locus_tag="ACG8V1_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330201.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4332..5834)
                     /locus_tag="ACG8V1_002116"
     CDS             complement(4332..5834)
                     /locus_tag="ACG8V1_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0330202.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6382..8061
                     /locus_tag="ACG8V1_002117"
     CDS             6382..8061
                     /locus_tag="ACG8V1_002117"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0330203.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8188..8667
                     /locus_tag="ACG8V1_002118"
     CDS             8188..8667
                     /locus_tag="ACG8V1_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0330204.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8886..9566
                     /locus_tag="ACG8V1_002119"
     CDS             8886..9566
                     /locus_tag="ACG8V1_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0330205.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9574..10905
                     /locus_tag="ACG8V1_002120"
     CDS             9574..10905
                     /locus_tag="ACG8V1_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0330206.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10920..11882
                     /locus_tag="ACG8V1_002121"
     CDS             10920..11882
                     /locus_tag="ACG8V1_002121"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0330207.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11993..12331
                     /locus_tag="ACG8V1_002122"
     CDS             11993..12331
                     /locus_tag="ACG8V1_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0330208.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12410..13117
                     /locus_tag="ACG8V1_002123"
     CDS             12410..13117
                     /locus_tag="ACG8V1_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0330209.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4209 a         2324 c         2889 g         3773 t
ORIGIN      
        1 agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag tgattttcta
       61 ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt atccattttt
      121 tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa acaaattctt
      181 caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat gtcattattt
      241 ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga aagttcagca
      301 cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact gttttacgtc
      361 aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat caattagaag
      421 aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa
      481 aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa atctccttga
      541 cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct aaaaaattgc
      601 ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc ttaaattttg
      661 tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc gctgcattga
      721 tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc gacgcaggta
      781 tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg ctattattct
      841 ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt ccattctcaa
      901 tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca gtaaccgtga
      961 cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt agcgtagcgg
     1021 tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga ttgattatgt
     1081 gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa aaaatccgta
     1141 aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct catatcaaaa
     1201 agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta ttagagatgt
     1261 tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac attccagaaa
     1321 tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt cttttcaaat
     1381 tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca gctgatttag
     1441 ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg ggaggatctg
     1501 atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg
     1561 gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg
     1621 ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct ccactggcag
     1681 atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt tcaggtttta
     1741 tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt gcacggatcg
     1801 aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt gttttaggaa
     1861 ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga cctgtaaata
     1921 aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg gtaaaatcaa
     1981 aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag ttatacttaa
     2041 agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca ccagattact
     2101 taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa gaacagctag
     2161 aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa gtactaaccc
     2221 cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc ggttatcaaa
     2281 atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg acagttgtcg
     2341 gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc aaagcgaaag
     2401 gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga ttgccggtaa
     2461 aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt atcggaatcg
     2521 gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag ttcgctcgtg
     2581 cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc atcaatgtcc
     2641 acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa gaaacagaaa
     2701 ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta tttgaagctt
     2761 gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa gatcctgatt
     2821 tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga ttgatcgagg
     2881 aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc ttgccttatt
     2941 ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca ttgctagtag
     3001 ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta cgagctataa
     3061 ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg
     3121 atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt atcgctaaag
     3181 gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa aaaagttttt
     3241 aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg
     3301 ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca caatggcaga
     3361 tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa aagaagcata
     3421 cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat cactaggtgc
     3481 tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa
     3541 agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa aaaatccgaa
     3601 ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc tgttttttaa
     3661 acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca gctttttgct
     3721 acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc gatttcttgg
     3781 aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg ctcatccgct
     3841 acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg ctgctgacct
     3901 cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac acgagccagt
     3961 cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc cattgctgct
     4021 atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa aataatcgag
     4081 acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc ttgatagtat
     4141 acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt cgtttttcca
     4201 gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag ttctacatct
     4261 ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt ccgtaacatc
     4321 aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat ccctatagaa
     4381 gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag tcccaattgt
     4441 gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa tggattattc
     4501 gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc tggcatttgt
     4561 ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc aagtagctgc
     4621 tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa tgcaagaatc
     4681 atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga ttctcctaaa
     4741 gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat gatcagagta
     4801 agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa acttgccacg
     4861 ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa gctaaatgta
     4921 tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc tggatcagat
     4981 agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag ttcatttgca
     5041 aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt catacccata
     5101 ctgccgcttg tttctctgga ttcataaatt cctttgatcg tcatttcata agtggtatct
     5161 tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt tgtttctgct
     5221 agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat tccttcacca
     5281 tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc tgacacacca
     5341 ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc agcaggttca
     5401 ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt gatggcagtt
     5461 attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt tacattatct
     5521 aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa actgcctgga
     5581 tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt tttaaatgaa
     5641 gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc ttgattcgct
     5701 gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag aatggctgtg
     5761 aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt ccatgcacgt
     5821 ttcataaaat tcatattttg catccctcct agtagatgca tttgataata gacaacctat
     5881 cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt tctgtatata
     5941 taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac
     6001 cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac agagggattt
     6061 ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat ttgttgtata
     6121 ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt cgcaaaagat
     6181 ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca gaacgcggat
     6241 gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt tgcatatgct
     6301 tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta aacttggatg
     6361 tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg cggcgttcgt
     6421 gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat tttagattgt
     6481 gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc tgattttacg
     6541 tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg acatgcagat
     6601 gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt cgggacagag
     6661 ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa aggatttaag
     6721 gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt agtgagcttt
     6781 ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac aaaagaaggt
     6841 agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca
     6901 gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact aagtgaatcg
     6961 gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga tgaagtattt
     7021 gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa
     7081 cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt cttgactggt
     7141 caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca attaccaagt
     7201 gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga actgatcatt
     7261 ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc aaacggtaca
     7321 caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac gccaagcatc
     7381 gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg tggaacagtg
     7441 aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga cttgcaatta
     7501 atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta tcgtgaatta
     7561 gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat ctatattact
     7621 ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg aactacaagt
     7681 gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat tgttttacgt
     7741 gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat cagtcggcga
     7801 gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta cgtaaaagct
     7861 agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga gaaacattta
     7921 gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca attaggccgt
     7981 tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat ggaagctact
     8041 caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata gagtgctcgt
     8101 cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc gctttataat
     8161 aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga acgttattta
     8221 gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga agaatctttc
     8281 acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac tctcgtagtg
     8341 aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca cttagattac
     8401 aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat ggattttgta
     8461 ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa actcgcacat
     8521 cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt aattgtgtca
     8581 agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa gatcgtccat
     8641 cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca gagcagagac
     8701 ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc tgttacaatt
     8761 gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta tattttttca
     8821 ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt
     8881 aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg gaaaatggcc
     8941 aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta actgcagaag
     9001 aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata ggagatatga
     9061 ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt gctgattatg
     9121 cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat gcgtttccat
     9181 tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta gacggtatcg
     9241 ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa acgatttatc
     9301 atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa ttcgatattg
     9361 atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat gcagcaaagg
     9421 ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact ttccctgtca
     9481 ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga aaaataatga
     9541 gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga tttgatttta
     9601 gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat cgcaaaagaa
     9661 ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta
     9721 gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa aaaacatatt
     9781 gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt aggcggggcg
     9841 gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa acatgccatg
     9901 gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga agtccaaaaa
     9961 ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac gactgctgag
    10021 attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta tgatacattt
    10081 acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg
    10141 cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca gacagatacg
    10201 atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac
    10261 cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa atcgccaaac
    10321 cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt
    10381 gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt cgtttccgat
    10441 gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat gcagcttgtc
    10501 cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa tatccatacg
    10561 attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt cgtagtagca
    10621 ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga aatcatttct
    10681 gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg
    10741 aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg tccacgtgta
    10801 ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga aaacaccatc
    10861 gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa
    10921 tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc attatccaca
    10981 ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc gaactattac
    11041 gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa gggcttcgct
    11101 ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta gtcatcgtga
    11161 ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca gaaaaactga
    11221 tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa
    11281 aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct gaatctttaa
    11341 agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat acagggcgct
    11401 ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg agaaattatc
    11461 catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac
    11521 tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga agaataatcc
    11581 ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct tcgatcgttt
    11641 cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag cagccttcag
    11701 ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg gctaaacgcc
    11761 atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag
    11821 aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga aaaacagaat
    11881 gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc ttttcaagat
    11941 cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga agatgacatt
    12001 accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct tgttgatttg
    12061 cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag aagacggatt
    12121 agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga cagtgattaa
    12181 agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat
    12241 ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg gaccgatgaa
    12301 actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt cacattcttt
    12361 tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta
    12421 tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat tagttatttc
    12481 cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa
    12541 aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac agataaataa
    12601 tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag gtaataagat
    12661 gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc
    12721 tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga agattcgtgc
    12781 agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga ttatggtagg
    12841 tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt tttctttagc
    12901 attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc gagcaatcca
    12961 aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa gacacgtatt
    13021 atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc agctgttgcg
    13081 cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg tgaaatcagc
    13141 gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt tggaa
//
LOCUS       DAZKMK010000077        12919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000077 DAZKMK010000000
VERSION     DAZKMK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.079"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG8V1_002124"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG8V1_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG8V1_002125"
     CDS             706..1734
                     /locus_tag="ACG8V1_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0330210.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG8V1_002126"
     CDS             1731..2489
                     /locus_tag="ACG8V1_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0330211.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG8V1_002127"
     CDS             complement(2575..3315)
                     /locus_tag="ACG8V1_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0330212.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG8V1_002128"
     CDS             3376..4629
                     /locus_tag="ACG8V1_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0330213.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG8V1_002129"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG8V1_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG8V1_002130"
     CDS             5300..5626
                     /locus_tag="ACG8V1_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0330214.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG8V1_002131"
     CDS             5694..6407
                     /locus_tag="ACG8V1_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0330215.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG8V1_002132"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG8V1_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG8V1_002133"
     CDS             7690..9555
                     /locus_tag="ACG8V1_002133"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0330216.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG8V1_002134"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG8V1_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG8V1_002135"
     CDS             11120..11821
                     /locus_tag="ACG8V1_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0330217.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG8V1_002136"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG8V1_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         4327 a         2176 c         2738 g         3678 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctg
//
LOCUS       DAZKMK010000078        12868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000078 DAZKMK010000000
VERSION     DAZKMK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.080"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1088
                     /locus_tag="ACG8V1_002137"
     CDS             <1..1088
                     /locus_tag="ACG8V1_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0330218.1"
                     /translation="KNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSNIEETSSS
                     EIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTIS
                     NIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKA
                     IKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQE
                     LEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKEL
                     SSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVL
                     ENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1160..2281
                     /locus_tag="ACG8V1_002138"
     CDS             1160..2281
                     /locus_tag="ACG8V1_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0330219.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2363..4135)
                     /locus_tag="ACG8V1_002139"
     CDS             complement(2363..4135)
                     /locus_tag="ACG8V1_002139"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330220.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(4138..5850)
                     /locus_tag="ACG8V1_002140"
     CDS             complement(4138..5850)
                     /locus_tag="ACG8V1_002140"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330221.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5965..6633
                     /locus_tag="ACG8V1_002141"
     CDS             5965..6633
                     /locus_tag="ACG8V1_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0330222.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6700..7488)
                     /locus_tag="ACG8V1_002142"
     CDS             complement(6700..7488)
                     /locus_tag="ACG8V1_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0330223.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7571..9043)
                     /locus_tag="ACG8V1_002143"
     CDS             complement(7571..9043)
                     /locus_tag="ACG8V1_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0330224.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(9173..10366)
                     /gene="metK"
                     /locus_tag="ACG8V1_002144"
     CDS             complement(9173..10366)
                     /gene="metK"
                     /locus_tag="ACG8V1_002144"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0330225.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10364..10443)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10632..10703)
                     /locus_tag="ACG8V1_002145"
     tRNA            complement(10632..10703)
                     /locus_tag="ACG8V1_002145"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10668..10670),aa:Glu,seq:ttc)
     gene            complement(10715..10788)
                     /locus_tag="ACG8V1_002146"
     tRNA            complement(10715..10788)
                     /locus_tag="ACG8V1_002146"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10752..10754),aa:Ile,seq:gat)
     gene            complement(10801..10871)
                     /locus_tag="ACG8V1_002147"
     tRNA            complement(10801..10871)
                     /locus_tag="ACG8V1_002147"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10837..10839),aa:Gly,seq:tcc)
     gene            complement(10876..10948)
                     /locus_tag="ACG8V1_002148"
     tRNA            complement(10876..10948)
                     /locus_tag="ACG8V1_002148"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10913..10915),aa:Phe,seq:gaa)
     gene            complement(10952..11024)
                     /locus_tag="ACG8V1_002149"
     tRNA            complement(10952..11024)
                     /locus_tag="ACG8V1_002149"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10989..10991),aa:Asp,seq:gtc)
     gene            complement(11043..11116)
                     /locus_tag="ACG8V1_002150"
     tRNA            complement(11043..11116)
                     /locus_tag="ACG8V1_002150"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11080..11082),aa:Met,seq:cat)
     gene            complement(11129..11218)
                     /locus_tag="ACG8V1_002151"
     tRNA            complement(11129..11218)
                     /locus_tag="ACG8V1_002151"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11180..11182),aa:Ser,seq:tga)
     gene            complement(11241..11312)
                     /locus_tag="ACG8V1_002152"
     tRNA            complement(11241..11312)
                     /locus_tag="ACG8V1_002152"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11277..11279),aa:Glu,seq:ttc)
     gene            complement(11334..11421)
                     /locus_tag="ACG8V1_002153"
     tRNA            complement(11334..11421)
                     /locus_tag="ACG8V1_002153"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11385..11387),aa:Ser,seq:gct)
     gene            complement(11446..11519)
                     /locus_tag="ACG8V1_002154"
     tRNA            complement(11446..11519)
                     /locus_tag="ACG8V1_002154"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11483..11485),aa:Ile,seq:gat)
     gene            complement(11533..11603)
                     /locus_tag="ACG8V1_002155"
     tRNA            complement(11533..11603)
                     /locus_tag="ACG8V1_002155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11569..11571),aa:Gly,seq:tcc)
     gene            complement(11605..11680)
                     /locus_tag="ACG8V1_002156"
     tRNA            complement(11605..11680)
                     /locus_tag="ACG8V1_002156"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11645..11647),aa:Asp,seq:gtc)
     gene            complement(11699..11772)
                     /locus_tag="ACG8V1_002157"
     tRNA            complement(11699..11772)
                     /locus_tag="ACG8V1_002157"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11736..11738),aa:Met,seq:cat)
     gene            complement(11785..11874)
                     /locus_tag="ACG8V1_002158"
     tRNA            complement(11785..11874)
                     /locus_tag="ACG8V1_002158"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11836..11838),aa:Ser,seq:tga)
     gene            complement(11898..11971)
                     /locus_tag="ACG8V1_002159"
     tRNA            complement(11898..11971)
                     /locus_tag="ACG8V1_002159"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11935..11937),aa:Ile,seq:cat)
     gene            complement(11985..12058)
                     /locus_tag="ACG8V1_002160"
     tRNA            complement(11985..12058)
                     /locus_tag="ACG8V1_002160"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12022..12024),aa:Met,seq:cat)
     gene            complement(12086..12158)
                     /locus_tag="ACG8V1_002161"
     tRNA            complement(12086..12158)
                     /locus_tag="ACG8V1_002161"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12123..12125),aa:Ala,seq:tgc)
     gene            complement(12178..12251)
                     /locus_tag="ACG8V1_002162"
     tRNA            complement(12178..12251)
                     /locus_tag="ACG8V1_002162"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12215..12217),aa:Pro,seq:tgg)
     gene            complement(12261..12334)
                     /locus_tag="ACG8V1_002163"
     tRNA            complement(12261..12334)
                     /locus_tag="ACG8V1_002163"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12298..12300),aa:Arg,seq:acg)
     gene            complement(12345..12430)
                     /locus_tag="ACG8V1_002164"
     tRNA            complement(12345..12430)
                     /locus_tag="ACG8V1_002164"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12394..12396),aa:Leu,seq:taa)
     gene            complement(12447..12518)
                     /locus_tag="ACG8V1_002165"
     tRNA            complement(12447..12518)
                     /locus_tag="ACG8V1_002165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12484..12486),aa:Gly,seq:gcc)
     gene            complement(12530..12602)
                     /locus_tag="ACG8V1_002166"
     tRNA            complement(12530..12602)
                     /locus_tag="ACG8V1_002166"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12567..12569),aa:Thr,seq:tgt)
     gene            complement(12620..12701)
                     /locus_tag="ACG8V1_002167"
     tRNA            complement(12620..12701)
                     /locus_tag="ACG8V1_002167"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12665..12667),aa:Leu,seq:tag)
     gene            complement(12709..12781)
                     /locus_tag="ACG8V1_002168"
     tRNA            complement(12709..12781)
                     /locus_tag="ACG8V1_002168"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12746..12748),aa:Lys,seq:ttt)
     gene            complement(12785..12857)
                     /locus_tag="ACG8V1_002169"
     tRNA            complement(12785..12857)
                     /locus_tag="ACG8V1_002169"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12822..12824),aa:Val,seq:tac)
BASE COUNT         4164 a         2730 c         2261 g         3713 t
ORIGIN      
        1 taaaaaatga tattatttta ttaaaaaaag agaaagaaga tttaaatgat gaaataaatc
       61 aaaagtatga tgaaacatta aaaaatgtct attcttcact tatcgtttca aatatagaag
      121 aaacttcatc ttctgaaata aaaaataagc tttctttgtt aaaattaaaa gaaaaggaat
      181 atattattga cgatgctgtt tctgtccctc ttattttaaa taaacgagag aaaaataatt
      241 taaagaaaaa aatgctcatt ccatttgatt cagaagtgaa aggattactc aataaactaa
      301 ctatttctaa tatagactcg actagggaaa aaatcataaa aatatttgat aaaattaata
      361 atttattcaa gaatgacaat gctgaattaa ggaagggtct tttagagttt aaacttcaag
      421 aactagaact tcattactct tatttagtga aaattaccga agaaaaagaa caacaaaaag
      481 caataaaaga acagatgata gaagaggaaa aagttcgtag agaaattgat cgtgaaaaga
      541 aacgtattga taaagaacaa cgtcaattca attctgaaat ttccaaatta atcgcatata
      601 tgcaaaaagc taatgcagat gtggaaaaag aattatatgc aaataaaatt caagaactgg
      661 aagaaaaatt aaaagaatta gaagttatac gtgaaaatgt attacaaaga gaattaaata
      721 ctagagccgg ttatgtgtat gtaattagta atattgggtc ttttggtgaa gacatatata
      781 aaattggtat gactagaaga ttagaaccta tggatagaat aaaagaattg agtagtgcat
      841 cggttccatt tgaatttgac gttcatgcga tgattttttc agaggacgct ccgtctttgg
      901 aaactaaatt gcataatcat tttagaaaac aagaagttaa taaaattaat caaagaaaag
      961 aatttttcaa agtttcacta gacgaaatag agaaagttgt tttagaaaat tataatggaa
     1021 cagtaacatt tacaaaatta gctaaagctg aacaatatag acgatcttta gaattgtcaa
     1081 aagattaaat atataaaaag ccttcgggct ttttctttta acaattaata ggaacgtata
     1141 ttcgaaagga atagaaataa tgtggttaga aaaaataaat gacaaaaaat ttaagtacac
     1201 cgaacgttat acggatccct taactgaaaa aaagagaaaa gtgtccgtta ctttatctag
     1261 caattcacgc caagcttgga atcaagctaa cttacttttg aatgaaaaaa tcgcagaaaa
     1321 aataaaacga aatgaagaat taccactaac ttttggagaa ttaaaaacta aatgggatga
     1381 aaaatacaag ccaactgtaa aagaaagttc ctatcgaaca actcaggttt atctatcatt
     1441 aatttcaaaa tatataaaag atgatgtact tgtaaaaaac gttaatagta atcttataca
     1501 agatatgcta gattactact attttgaaaa aaatttatca tataattatg taatacattt
     1561 aaaaactttt acaggaatga tttttaaatt cgccaaccgt cgatatggat taaaatataa
     1621 tcccgtaaat ggggtttctt tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga
     1681 aaaagatggt tatttatcaa aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc
     1741 acatcaacag tcacgttatt ctttaattac taaatttttg tttttaactg gactacgtta
     1801 tggtgaattg atatctttga ctgaatctga ttatgatggt aataaaatta cggtttctgg
     1861 tacttatgat tacgagttaa aaattaaaac aacaactaaa aatacaggtt cttatagaac
     1921 cattgaatta tcaagtaatg ctaaagaaat catagatcaa ttaattgaag aaaacaaatt
     1981 aattaagaat aataaagata aatacatttt catctctaaa aatggtaacc cgatatctat
     2041 tcaagcatat aatcaatctt taagagcagt atccaaagaa ttaaacctag ataaaaaagt
     2101 ttcttctcac atgttaaggc attcccacat ttctctactt acagaattag gaatcccatt
     2161 aaaagccatt atggatcgtg taggacatga agattcaaaa acaacattaa agatttatac
     2221 tcatacaact aaaaatatgc aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta
     2281 aattgcccct tttctgcccc ttataaaaac aaacaacata aaatatataa aaagaacccc
     2341 gttataacga ggttctttta atttaacttt caaatacaaa ttgattatga taaagttccg
     2401 cataaaatcc attttctgct aataactcgt gatgactgcc ttcttcaatg acttccccat
     2461 cacgcaagac aacgattcta tctgcattca agattgtttt cagccggtga gcaatcacaa
     2521 agctggttct tcctttgatt gcttcatcca ttgctttttg gatccttgct tctgttaccg
     2581 tgtctacatt acttgttgct tcatccaaaa tcaataaaga tggatttgtg atgatcgttc
     2641 gagcaatact gatcaactgc ttttgacctg tactgaataa gttattttct tcggatatct
     2701 ctgtatcata acctttttct aacgtcatga tgaagtcatg aatattcgct tgttttgctg
     2761 cattgatgac ttcttcatct gaagcgtccg gtttgccgaa tgtgatattg tctttgatcg
     2821 ttccggagaa caatacagat tcttgcaaaa cgattcctac atgtctacgt aatgcatcaa
     2881 gatcataatc tcggatatct attccatcga tgactactga accatcattg acatcgtaaa
     2941 aacggtttag cagattcatg atcgtcgttt tccccgatcc tgttggacca acaagagcaa
     3001 ccatttctcc tttgtccaca ttgatcgaga catctttcaa gactagtttt tctggagaat
     3061 aaccaaaatc tacgtgctgc aacgcaagac ctttttcaac accatcaaat gctttacagt
     3121 tttctggttt gacttcatct ttttcatcaa atatttcatt caatcgtctt gcccctgtaa
     3181 tcgctaattg gatcatgctg tatccagatg aaatctgcat cagtggttgg taatattgtt
     3241 gtgaatactg gacgaatgtc acaactaagc ctaatgccac cgaacgttcc aaatcaccat
     3301 ttaatgctaa ccagccgccg aaaaagataa ctatcgctgt attgaccagt gacattcctt
     3361 gcatcatcgg aaatagtaga ccagaataag cttgtccttt gaaggttgct tcgcgaacac
     3421 tttgattatg ctctaagaat ccttcaatcg tttcttcttg taagccattt gtgatgatga
     3481 cacgttgtcc gctgattttt tcatccatat aaccatttag tttccccact tcatcttgct
     3541 gaagatccac atatttccgt gctttgcgga taatcaagac agctatcaaa acggctactg
     3601 gagtggaagc aattgtagcc caagctaact ctacattttg gcggaacatc atgatcaaga
     3661 tcccgacaag taaaacagca ttcgtgatga cttgaagcaa agcttggttc aaactatttt
     3721 gaatattatc taagtcactg gtaaaacgac ttaaaatttc accgtcttga tgagaatcaa
     3781 agaaacggat cgtcaatttt tccaatttgt tgaatagacc gatacgcatt cggttcgttg
     3841 acttccctac tacttgtgtg aacaaaatac tatagataaa gttggctgca ctagctagta
     3901 cataaaacag caagagtttc catattacag aaacaaattt gcttttatcg tctacgcctt
     3961 gcatcaagcc tcctacgtaa ttagctaatt cttgaaaagc ttccccgatg tattgaggtg
     4021 cctttacttg tagataggta gcaatgatca ctgtgataaa gataaagaaa aaggagagtt
     4081 tgtaccgctt caaatagtga tagaaaaatt tacttgcttt tattagatca gtcatactta
     4141 ctcctccgtt cctttttgag tttcaaagat ttcctgatag acacggtttc cagctaccaa
     4201 ttcctcatgg gttccttcgc cgactaatct gccttcatcc aatactaaga tgcggtctgc
     4261 tttgacaaca gaagcaattt tttgtgcgat gacaatcgtc gttgttccct tcaaatctcg
     4321 atcgagtgct tctcggacca gcttttcaga gcgagcatcc agtgcactcg tactgtcatc
     4381 taggatcaag atctttggat taccgataac gccacgagta atggataaac gttgcttttg
     4441 accaccagaa aagttactgc tacgttcttc tattggtgct tcgtaacgat cagataactt
     4501 ttcgatgaat tcttttgctt gagcaataga agaagcatgt tccatctctt tttctgttgc
     4561 attctttttc ccttggcgca agttttgagc aatcgttcct gagaataaaa ttgctttttg
     4621 caaaacaaag gaaaccgttt gtcttaaact cttttcattt acttgacgaa gatctacacc
     4681 acctacttca actttgcctt ctgtcggatc aaatagacga gggatcaatt gtgctagtgt
     4741 tgacttcccg gcaccagtcg ccccgacgat accaaccatt tctccaggtt tgatagaaaa
     4801 gctgatatct ttaagcgtat ctgtatcctc accaggataa cgaaaagaaa catggtcaaa
     4861 agtgacagaa cccgtcaact cttgttctgg aacgtctaaa taagtaagat ctggttcagc
     4921 atctaaaatt tccgcaatcc gcttcagcga tacggctgct cttgaagtca tcatcatcat
     4981 cattccgcca ataatgattg ccatcataat ctgcatcaag tagttcatga atgaagcaat
     5041 tccaccaatt aacgtaggat catctttaac aagatcgctc acaaaaaaga tcgccccaac
     5101 cacagccaaa ttggcagcca gcatgaagga cgggatcatc acagaaaata atgtaccgac
     5161 tatgatgttg tgttttgtta agtcctcact tactttgctg aatgacttca attggttttc
     5221 ttcttgtaca aaagatttca ctacacgaat ccctagtaag ttttcttttg caatgctatt
     5281 cacacgatca atcagttttt gaatgatcat gaagtgcttg cccatccttg taaatgaaag
     5341 tgccgtaatc agaaataccg taacgataag cgctacgatg atccaccata attgcggcat
     5401 cgtgatcata gctaaaatga aggcaccaat aaataagaat ggaattcgaa aaagtgattg
     5461 caaagaaatc atcactaaat tttggatttg cgtgatatca tttgttagac gaacaacaag
     5521 atttccagca gagaactgtt cgatatttcc aaatgaaaat gtctggatct ttcgaaatgc
     5581 ttcttctcgg atatctgcac ttacgccttg agctactttt gcagagaaaa tcgtgttcgt
     5641 tacacctgcg gctaatccta gcaaagccag actgatcagg taaataccaa ttgttttcat
     5701 ttcatcatta tcttctgtaa taatagcttc caatacttgc tgtagtaact taggttgcca
     5761 taatgctgct gcaaccatga ctgctacgga cagcaaggaa ataaaaacag ctactttata
     5821 ttttttcgca tgtcgtaaga ttaatttcat tcgttgatta cacccctttg tttaaattga
     5881 caggactaat ttaacactgt aaaattagac cgactaacaa tttaccatgt ttaaaaagat
     5941 aatgcaatac acgaggttga taaaatggaa ataaagcttt ctcaagcaat catcattcag
     6001 gccgcctttg atatcctggc tgaaacacaa gctctttcgc agctttccat gcgaaacatc
     6061 gcaaacagaa ttcatgttca agcacctgct ttatattggt atttcaaaaa caaacaacag
     6121 ctcttgcaaa taatggctga aacaatggaa gaagaattag cgcttccaga tccaactttg
     6181 ccttggaatg aacagttgct gcagtatatg gaaaattact atgaccttta tacacgtttc
     6241 ccttgtgggg cagaattaga aatcaatact gtgcccgctt ttccgagtcg attagaacat
     6301 ttggagcaaa tgatccaatt acttgttcgt gaaggatttt cagtcaccca aagtcatcag
     6361 gccattgcct ctctccacaa tttattgatt ggacagttaa tggatcagca gcatgaacag
     6421 cagcttcgac aaaaaattat ggacggaaat gaatttttga aagaaacagt tctatttatg
     6481 cgtaattatg tagttgaaaa tcaattagag ggatttaaag aaagtatgca gtcccatgca
     6541 gaattgaatg aaaaagcact ctttctaacg agtgcacaaa cctatattga aggcttatgc
     6601 caacaaaata aaagaagaga aaacgagcag taaaaaagct cctatagaga tcactatgtt
     6661 tgtgatctct ataggagcta tctttttaag actctttttt tatttttctt ccattagcca
     6721 cgccaatgca cgttgaatcc attggtccca gaagatccag ttatgatcgc caggtccttg
     6781 ttcaaaggtg accgccaagc cttgttcctg catagcagat accatttcct gatttgcctg
     6841 ataaagaaaa tcagattccc cacaagccat ataaaatctt ggaactggtt ttccgctttt
     6901 tactagttct ttcaataagt attgcggatc attttttgaa ccttcgattt gatccaatgg
     6961 tccaaagatc ccttcccaaa aagatgcttt acgaaccatc aaaagatcat ccattcgatt
     7021 tcctacagaa acagcgcctg aaagagagat tactgctgcg aaattttctg attttgcaag
     7081 gcctagtttt aacgcgccat atccgcccat cgataatcca gcggcatatg ttttttctct
     7141 ttttcttgtt aattgcggga ataattcatg acaaatctca ggtagctctt ctgcaatgaa
     7201 tgtccaatac ttcatgtcat aaactgtatc tgtgtaccag ccaagatcag tagatggcat
     7261 gatgacagct aatccgtagt ttgctacata acgttcaatc gacgtacgtc tttcccatac
     7321 actatgattg ccacccattc catgaagtaa atataaaaca ggtatatccg ttgtctcttg
     7381 ctctgaactg gtgccaatca ccttttttgt tgtttgcggt agtatcacat ttagattgac
     7441 ttccatttct aacacattcg aataaatatt tgcttgaata aatgccatat tttcgccttc
     7501 cttagtaaga agttattttt gaataacttt aaatttgaat gattcggcta cgtacatatc
     7561 ttaaccgaaa ctactgtttt attttaccac ttttttcatg aacgaaaagc gttaagacca
     7621 aagcaatgat cacgagtaac aagccaacta gataaacgga atgtaaccca tcataaagaa
     7681 tttttcttaa tggtaacaaa atatccgcat caatatattt agccgtttgt ggattcacca
     7741 gtttattcat gatatctgca tcatttgtta aatttgctgc tgcaagttct cgtttagtta
     7801 ccccatttat caataaacca aaaatagaaa ccatgactgt ttgaccaatt gttcgaacta
     7861 acgtattaaa agaagttgct acaccaagct ggtcacgaga tacactattt tgtgcactga
     7921 cagtcgaggt agtcatggtc acacctaagc ctataccgat gaccgttgat atagcaaaaa
     7981 accataaata gttacttctg aaaggcataa gatacaacca agcgccccca atagctgcga
     8041 ttagtagtcc aattctcaag acccaatgag ttttcatttg agtcatccac ttgcttgcaa
     8101 ggaaagaacc gaccatccag actagtgata atggtgctaa taccagccca ccaattgctg
     8161 cattcagccc taatacacct tgcatccaca tagggatata aacatcgatt cccatcaaaa
     8221 aaccactagc caatgctgca gccaagttga ccacaacaaa tagtggattt ttgaataaat
     8281 gcatcatgat gacaggatct ttagctcttt tttcggcaaa aacaaaaata cctaacatta
     8341 cgatactacc tagcagcaaa gcagaaacaa gcggactcat tccttcgtct cctaataatt
     8401 ggaaccctaa cagcaatgag acaagcatgg ccatcaaact aaaactacca aaaatatcca
     8461 ttggtttaga ctcatgtatg atttttggtt catttaaata tagccaaatc aaaataatta
     8521 atagaattcc gatcggcaca ttgataaaaa agatccagtg ccagctgatc gtttcaacga
     8581 tgaatcctcc cgcaagcggt ccaaaaacac tggcaatccc ccaggctgaa ctattcaaac
     8641 ctaaaacttt tgctcgcttt tcaatagaat aaagatcgcc gatgatcgtc aaagctactg
     8701 gcatcattgc cccagcaccc atcccttgaa tcgcacgagc agcaatcaac ccaatcatgc
     8761 tttgagataa accgcaaaat aacgaaccga taataaacaa taatgtccca aaaataaaga
     8821 ttggtttccg tcccacttta tccgcaagtt ttccgtaaat cggagtaagc atagcgtttg
     8881 tcagcaaata aatcgaaaat acccagttca tgatttccat tccatgtaat gaaccgacaa
     8941 tcgttggcat agcggttgtt acgattgtac cttcaatagc cgtcatgaac gttgcaatga
     9001 aaatactcac tgtgaccaaa gtaacatttg tttctttttt catcttattc ctccctttca
     9061 ttatgacagg atctaaaaat agataaataa ttactatctt tttcaagagg ttaactgcca
     9121 taaaaaaagc tggaacaacc accacttggc gatcgttcca gctgaatata acttattttg
     9181 ctaaacttgc tttcaacgca tctactttgt ccgtatgttc ccaaggcaaa tctacatctg
     9241 tacgaccaaa atgaccgtaa gcagctgttt gtttgtaaat cggacgacgc aaatcaagca
     9301 tctcgatgat tcctgctggc cgaagatcga agttttctct cactgcagca atcagttctt
     9361 cttccggaac cgttccagta ccgaaagtat tgattgagat cgaaacaggt tgagcaacac
     9421 caatcgcata agctagctgt acttctgctt tgcgagcaag ccctgctgca acaatgtttt
     9481 tagcaatata acgtgcagca tagctggcag aacggtcaac ttttgtcgca tctttaccag
     9541 aaaaagcacc gccaccatga cgagcataac cgccatacgt atcaacaatg atttttcttc
     9601 ctgttaatcc ggcatcccct tgaggaccac caatgacaaa tcggccagtc ggattgatat
     9661 aatatttcgt ttgatcatct aataattcag ctggaataac ttccttcact actttttcga
     9721 tgacatcatg acgaattgtt tcattatcta ctgcatcatc atgctgtgtt gaaataacga
     9781 ttgtatctac gcgttccggt tgtccttgat catcatattc aaccgttact tgagattttg
     9841 catctggacg taaatacgtt aattcatttg atttacgcaa atctgccaag cgtcgtacca
     9901 gacgatgact caaagcgatt ggtaaaggca tcaattctgg tgtttcatct acagcaaacc
     9961 caaacattaa accttggtca ccagcaccaa tctcatctaa tacgtctttt ttatcttcat
    10021 cccggatctc aagtgcttca tcaactcctt gagcaatatc aggagattgt tcatcaatcg
    10081 caaccaatac agctgctgta tctccatcaa atccgaattt tgcacgtgta taaccaattt
    10141 cttttattgt ttcccgtacg actttttgaa tatctacata tgccgtcgta gatatttctc
    10201 caaatactaa aacaagacca gttgttacag acgtttcaca cgctactcgt gctgttggat
    10261 cttgttttaa aattgcatcc aagattgcat cactgatttg gtcagcaatt ttatctggat
    10321 gtccttcaga aacagattct gatgtaaata agtgtctttc taccattata atgattcccc
    10381 ctagttaaat tcggttacaa ggcatctccg tcctgcggat cctttcggga acttgcaacg
    10441 aaccaagtat agcagataaa acagttttct gctcgtattt tttcattttt ttgcttaaaa
    10501 aatataaaaa tattttacat tagaactgtc caaaaaagct tagcacaatc atccataaag
    10561 ataactctgc taagcttttt aaaatcattc tttcagataa aaagaaaaag atactcaaaa
    10621 agtatcttcc atgacccgta cgggattcga acccgtgtta ccgccgtgaa agggcggtgt
    10681 cttaaccgct tgaccaacgg gccaataaaa taaatgggcc taaatggact cgaaccatcg
    10741 acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat aaaaactaaa
    10801 agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtggtt ttaccactaa
    10861 actacacccg cataatggcg cgggacagaa tcgaactgcc gacacatgga gcttcaatcc
    10921 attgctctac caactgagct accgagccaa acggttccga cgggaatcga acccgcgatc
    10981 tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccaatgtt tttagttttt
    11041 aattgcggga gcaggatttg aacctacgac cttcgggtta tgagcccgac gagctaccag
    11101 actgctccat cccgcgataa tatgaaaaag gaggataagg gattcgaacc cttgcacggt
    11161 tttacccgcc tgacggtttt caagaccgtt cccttcagcc ggacttgggt aatcctccat
    11221 gtaaaactat attaaattaa tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa
    11281 gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaaa caaaatattg aaacggagaa
    11341 ggagggattt gaaccctcgc gccggtttcc cgacctacac ccttagcagg ggcgcctctt
    11401 cagccacttg agtacttccc caaaaccaat aagattttgt actaatgggc ctaaatggac
    11461 tcgaaccatc gacctcacgc ttatcaggcg tgcgctctaa ccagctgagc tataggccca
    11521 taaaaaacta aaagcgggtg acgagaatcg aactcgcgac aacagcttgg aaggctgtgg
    11581 ttttaccact aaactacacc cgcatggcgg ttccgacggg aatcgaaccc gcgatctcct
    11641 gcgtgacagg caggcatgtt aacccctaca ccacggaacc agtgttttta gtttttaatt
    11701 gcgggagcag gatttgaacc tacgaccttc gggttatgag cccgacgagc taccagactg
    11761 ctccatcccg cgataatatg aaaaaggagg ataagggatt cgaacccttg cacggtttta
    11821 cccgcctgac ggttttcaag accgttccct tcagccggac ttgggtaatc ctccatatga
    11881 aacttacaac gccacaatgg accttgtagg actcgaacct acgaccggac ggttatgagc
    11941 cgtctgctct aaccaactga gctaaaggtc caggctctta aaaatcgcgg cggaggggat
    12001 cgaacccccg acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgcgaa
    12061 aaagatattc attatttaaa tgaaatggag cctagcggga tcgaaccgct gacctcctgc
    12121 gtgcaaagca ggcgctctcc cagctgagct aaggccccgt gtttaaattt ataattatcg
    12181 ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga
    12241 gctacttccc gtttaattaa cgcgcccaag aggagtcgaa cccctaacct tttgatccgt
    12301 agtcaaacac tctatccagt tgagctatgg gcgcatattt gaaatgccga ggaccggaat
    12361 cgaaccggta cggtgatcac tcaccgcagg attttaagtc ctgtgcgtct gccagttccg
    12421 ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg ttcgaacccg cgacccccac
    12481 cttggcaagg tggtgctcta ccactgagct atttccgcgt gtttaaagat gccggctaaa
    12541 ggacttgaac cctcgaccct ctgattacaa atcagatgct ctaccaactg agctaagccg
    12601 gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac ccccacgccg taaggcgcta
    12661 gatcctaaat ctagtgcgtc tgccaattcc gccacacccg caaaaatatg agccgtacag
    12721 ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc taccaactga gctaacggct
    12781 catatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
    12841 agctgagcta aacctccatg gataactt
//
LOCUS       DAZKMK010000079        12844 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000079 DAZKMK010000000
VERSION     DAZKMK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.081"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1331)
                     /locus_tag="ACG8V1_002170"
     CDS             complement(<1..1331)
                     /locus_tag="ACG8V1_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0330226.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSD"
     gene            complement(1428..1928)
                     /locus_tag="ACG8V1_002171"
     CDS             complement(1428..1928)
                     /locus_tag="ACG8V1_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0330227.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            complement(1941..2162)
                     /locus_tag="ACG8V1_002172"
     CDS             complement(1941..2162)
                     /locus_tag="ACG8V1_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330228.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            complement(2167..2304)
                     /locus_tag="ACG8V1_002173"
     CDS             complement(2167..2304)
                     /locus_tag="ACG8V1_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0330229.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(2301..3485)
                     /gene="mobT"
                     /locus_tag="ACG8V1_002174"
     CDS             complement(2301..3485)
                     /gene="mobT"
                     /locus_tag="ACG8V1_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0330230.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(3741..3995)
                     /locus_tag="ACG8V1_002175"
     CDS             complement(3741..3995)
                     /locus_tag="ACG8V1_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330231.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(4020..5162)
                     /locus_tag="ACG8V1_002176"
     CDS             complement(4020..5162)
                     /locus_tag="ACG8V1_002176"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0330232.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(5222..6574)
                     /locus_tag="ACG8V1_002177"
     CDS             complement(5222..6574)
                     /locus_tag="ACG8V1_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0330233.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(6645..6989)
                     /locus_tag="ACG8V1_002178"
     CDS             complement(6645..6989)
                     /locus_tag="ACG8V1_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0330234.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(6999..7373)
                     /locus_tag="ACG8V1_002179"
     CDS             complement(6999..7373)
                     /locus_tag="ACG8V1_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0330235.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(7386..7700)
                     /locus_tag="ACG8V1_002180"
     CDS             complement(7386..7700)
                     /locus_tag="ACG8V1_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0330236.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(7814..11041)
                     /gene="fss3"
                     /locus_tag="ACG8V1_002181"
     CDS             complement(7814..11041)
                     /gene="fss3"
                     /locus_tag="ACG8V1_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0330237.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(11120..11782)
                     /locus_tag="ACG8V1_002182"
     CDS             complement(11120..11782)
                     /locus_tag="ACG8V1_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330238.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            12399..12629
                     /locus_tag="ACG8V1_002183"
     CDS             12399..12629
                     /locus_tag="ACG8V1_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330239.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            12635..>12844
                     /locus_tag="ACG8V1_002184"
     CDS             12635..>12844
                     /locus_tag="ACG8V1_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0330240.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
BASE COUNT         3633 a         2840 c         2105 g         4266 t
ORIGIN      
        1 aaatcagagt tatatccatc tagtaattct agtatactag tttgattttt tgaaatttca
       61 taaatttgtt tttgagagta tatactcacc ttatcggaaa tcattctcaa ccgttctgtt
      121 tctttttcaa ccagaccatt ctcttgtgta aattcataaa tagaatgtct atcctgtgtt
      181 cttattattt gatattttgt ccccttatag aaaatattca gtttcacaat tgtatccttc
      241 aaaaatattc cagagccatt atgttcaatt tgtgcaaagt tctgataatc actttgtatt
      301 tcagaaaatt gatcaagttc tgcttccttt ccaaggacat agaccaagaa tcttgtaata
      361 caagatttgc ctgttcctct tcctccaata attgaagtta aattatcgct gaattcgact
      421 acaacttctt ttgtgctaag agtggtccca gaaaagctta atttttccaa taaaacttca
      481 ttttcattaa tggagaaatt agaaaaatca gaatgaactc gagtttcgcc tagcattaaa
      541 gcatcgcgaa gtgatcccaa gtcaggttcg tccttcattt ttatatagga atatcttgtg
      601 cctattcccc aaagtccatg ttttgaattt cctttcgagt gtgggttatc agaaaagcta
      661 agaaatgtca tattattttt ttctgcctca acaactgatt tataccaaga ttccaaagta
      721 tcttttccaa ctctaccatc atatctggca ttcacactat catagatagt gtcacgctta
      781 gatgagggaa tcatttgtga gcgattttca aaaaattctt tttgaaccgt ctgtactgca
      841 tatatatttt ctgattcaaa tatctttcct agatttccaa attttgctga agaaattcca
      901 ttaaactcat ctatatgtgc aggaataacg agcaagccct cgtgctcagc aaaatcaata
      961 acttctaaaa ctgatttcat tgagcaagct gcagcgctgg cccaatcctc tctttttata
     1021 cccatcttgg ttaagaaatc agagatatca tttgtacatg cacttttatc aaataggact
     1081 agtaaatgag ttccattttc tccacacgtt aattccactc caggaaaaaa atctaagcca
     1141 ttttcgatag ccactttctt atattcatca atattttccc ctgtattgtg atcagttaat
     1201 gcaacacaat cgagtccttt gtttaaacat tcttctaccc attccttagc agtaaccttt
     1261 ttatcctgaa agcactgact cgcagttgaa tgaatatgaa gatcacattt gtaccactta
     1321 ttgccaacca ttagtaacac ccctatcaac aaatattttc tatttcagta tatcaaatag
     1381 aaaagtaaaa gtacgataga aatttctggt caattctaac aaatgattta cattagttca
     1441 aaaacccctc gactcgtaac caaataattc ccgccaatct ccaagtctcg accacacgca
     1501 tcaaaatcaa tatacgtctt caagtgttct ggtacttcgc ctaaatcacc agcttctaca
     1561 atcaagtaat acgctacatc actcatgtca ttacaatcgg gataatagat gatatcatcc
     1621 acatgctcta ccatttcctc gaaagaattg aagaaggcat gttgaatctc tgaagcgacc
     1681 tcaccaattg gcgttccttt aagctcttcc gctaaaccac acaaccgatt aatttcctca
     1741 attggtgtat actcatcaat ctcaaaaggc agttcataat catgaattgc gtactcttca
     1801 tattcgtcat ttaatccaat ccgttctttc acttcatcaa aatcgacggg tggggtaaac
     1861 catgccccca ccaactcacc ttcattgtat tttccaaggt tcgcaatata aacacgtatc
     1921 tgttccattg tgattccccc tcaggcaccg acaattcggt taaacaaatt gagtaagaca
     1981 tccttcacac cagaagcatt aaagaccaga cctaccgcaa tcaaagcaat caccaagaag
     2041 ccaatcagtt tggaaaactc gcgtttaaat cctaaaaaca aaccaatgac cacaatcgct
     2101 agtaagacta aagattgagc attggttaaa aaccattgat acaaattttg accgaaattc
     2161 atttcatcac tttcctttct tttccattgc tcggtccgcc gctgcacttg catgcatcaa
     2221 gcacatcaca gtaacaccaa gcatactgcc accaaacacc aataaaatat ctttcacaat
     2281 ttccatcacg attcctcttt tcatataatc aaattttcaa ttgccatact ttgttgctcc
     2341 aaaatctttt tgtgccggtc tgatagtcga gtattattta ccatatcctg aatgtacgtt
     2401 gtctgattca accgatccaa ctccttggcc atcttccacg taggggcgac ttgtcgtcca
     2461 agccagttca acgttcgttc aatggtataa ggcttcggtt cagaagttaa ttgaatttcc
     2521 ttctgattcc gtccaatgaa gcgcccccat tgttcattca agggccagtt agttcgcctt
     2581 ttgccttcca ccttatccgc aaatcgcaag taccggttaa taatggaaaa tgtggtactt
     2641 tcaatactgc gaccttttaa caaatcttga attgcatgat aagctcgatc atttttcagt
     2701 cggatttcaa aacgattttt cgtttcggtc tggtctaaag gaatccctaa cttgatatat
     2761 tgctcatagt ctttttcgta caaacagaaa tagacctcac tcttaagact gccaatatac
     2821 aaagtattgc ccataccatc tttatcatca ggtttcaaca gttccccgga acgatagctt
     2881 ttgaaggtac gaaacaagga gatacattct tccttctgac attttttcgt taaatcagga
     2941 atatctaaca ggccaactcg atcattgatt gccaggtcta agcgtttcat cacgccaccg
     3001 gcttttagac aatcttcgaa aaagtcatac cagctacgct tttgggcgag taaaaaagtt
     3061 tcaaactgcc gacaaccacg accctttaat tctaaaagga cgcctttatc ttcttcatgt
     3121 gacaacatca ccacaatatc tcccataaca tattgttctt gataggagta aaaggcgtaa
     3181 tcttcgtgga gcatataatc aaatttcaaa tggagaatct cttgaatgat tttcctcgca
     3241 tccgttgtgg gaaagcgaat ccttacatag tcaaacagta acgttaaagg taactcggga
     3301 ttgaaacttt ccagttggtc aaaaattttc ctcatggttt ttgtcgatgc ggttacgcca
     3361 ccattttcta tctgatttaa gtacgggcga ctaagtccac aagtcgtagc taatttattt
     3421 tgtgaaacgc catattcatt acgccggtct tttaatagcc gataatctaa attcctttgt
     3481 gccaagttct cacctccaaa tttaaggtgt taactttttc aaatcagtta acatctacac
     3541 aaattgctta ttatccttga taggacagcc attccttatc gttgcttgct tattttttgt
     3601 gtttttgtac ccccctgtta gatacggggg gtgagacatc atggccggct aacgccgtcc
     3661 ataaacaggc acagcaccac ggaggatttc tgcttcgcaa atcccccgcg gtcagtgcct
     3721 atcagtggtt tatttggccg ttactccgcg gtaataccct caatttccaa ctctgctttt
     3781 atctcaaaca agtattggcg gatatcaggt tctttagcaa agtccgcaat tcgttccaat
     3841 tcactgatga cttgattcag ctccattcga gcatccgttt cctcatcaat tacatacgga
     3901 tcatcaggga ctttcacccc gtttttgcga atatcaaaat tgagccggta cagttcttct
     3961 tccaattccg gatctgactg aatcttgcca atcacccagt agattcccca atcaactcat
     4021 cataaatcat ctgtttttac ctcctttaat cttctagcaa tatcaaaaat gaccggaact
     4081 gttacagaat ttccagcctg cttatacaac tgactatctg aattgacttg agaagctcga
     4141 tcaaaagccc agtccggaaa gccctgaagc cgccagcact ccctaggtgt taactttcga
     4201 atttgatact gatccgtgac gactgcttgt tggtctcctg ttaataaggt atgcacactt
     4261 tgttttccca gacggcctcg acgacttgtg gaattaggaa aagccaggtt cacactgttc
     4321 cctggcttca cctcttgata ttctgtttcg tttttccgaa gcatgatctt cgttccgcct
     4381 tgtttcgttg acaaagtagg cgctgttcca gttatctcat aaacccgatt gccttgttca
     4441 aaatttccca gtaacatacc cgctacagaa attccatcat ctaccggcaa tgcgattttg
     4501 attccttcat caccggtcaa ggtaggcgct acgtcatctg acccatagac ttgtcccccc
     4561 attcctcgat tggaagggtt gatatttaat aagtttctga tgttttgatc agatgttgga
     4621 gacgatccaa tgataggaaa tacttgttcg gtacatgcgt ctcgagaatg tccgataagg
     4681 aagatccgct cacggttttg gggaacgtag gcctttgagt taagcacttg ccattccaca
     4741 tcatacccca atccatccag cgttcggagg atcgtctcga acgtagcccc tccttcgtga
     4801 ttgagtaatc cccggacgtt ctcaaggaat aaaaacttag gttggagaat agcggcgaac
     4861 cgagcgattt caaagaataa agtacctcga gtatcggaaa atccttgccg ctttcccgca
     4921 attgaaaaag cttggcacgg aaatccaccg caaaggatat ccactggtcc gagagattga
     4981 ataaactcgt ctgatacact tgtgatgtca tgcatttcca cctccttact tgtatcatgg
     5041 attgccttat aactctggcg agcaaaatca tcaatttcac aaaagccgat acactggtga
     5101 cccgcttgtt ccatacctaa gcaaaagcca ccaatcccag cgaataaatc taaaaatgtc
     5161 ataaatcacc tcgtaaaaaa agcccaggat tcttgacact cctaggctac atactattat
     5221 tttatccttc cgtattaacg tttaatccac caatgtcgct aatttccctt aagaaatcat
     5281 atccttttgg tacaagtggc gtataaaact cactaatcac actccctccc gtatctacat
     5341 agcctcgacc tttgatacgc ttcataaaga attttttatc aacctcgcca aacatcatgg
     5401 aatatcccag ttcactcata cgacccaaag ccacccagaa attaaattga tcgcgaatcc
     5461 cgtctcctaa atatttcgca tccggtcttt gacaagctag aatcagaaag aaaccagatt
     5521 gacgacctaa catcacgatt tgtttgagct tattcaaaat caccgcactt tccttcgtcg
     5581 ttaacatttc catataaacc acgtattcat caaagattaa aaagtttggt ggaagtccaa
     5641 gaaacgcata attctctcct gttttgtagt tgggcatttc tttcattgcc ttgctacgag
     5701 tcatcatgcg ctcatagaaa tcttccacac aagcagaaat ttcctccttt tgggaataga
     5761 catgaggcat taccgtacct aaatcggcta agtccgcatt tttgggatca agaacaaaaa
     5821 gctcggcatc agattttaat aaagcctcga tgatagtgag taaaaagtaa gtcttcccac
     5881 cacctgtgcc gccagcgatt aacatatgag gtaaagaatc ataagcccat acttgatttt
     5941 tcatcaatcg caaagcccca ctttctgcca ctacttcgtc tatcccaatt cgattggcaa
     6001 ttatatcata cagtaaagtg tactccacat aggagtcttt gagttccttt tctactagtt
     6061 cacaatatag accactttct aacttctttt ccagctttaa aagctgatct tgatattttc
     6121 ccagtgaaat ttgaacgcga atagaaagta aaccatcctt catacggtaa tagattttgg
     6181 ggaagtaact gattgtctct ttggttcgac tactattgag gtctttgaaa aatccttcac
     6241 tctgagtttg ttttacttcg taccaatgat tttctagaat catccgggct agcttttgtc
     6301 gatgccacaa actgcgccaa tgatcctgaa aaaagtgtat ataaagcaac atcgtgcttc
     6361 ctgaaagaac gagacttcct agcaaataga ttaaatcttt tatagaacga acgatttcta
     6421 tagtagaaag tgatagctta gaaatatcaa attgtctaag ctcttgaaaa taaaagacgc
     6481 tcatccaacc gatgaacaga gcgagtagcc agccaagaac aaagtgataa accaagtgct
     6541 gatctcctgc ccgtatacga tgccctttgt acatttttat ggtttccacc tccaatcgaa
     6601 aaaagcttca taggatttca ttgatccaat ttcaagcagt tatgctagtc atttactgct
     6661 atagggagtt gtttcgtttc cactagttgc tttaaaaaaa cttcaatggc atccggcatt
     6721 tccacaccaa tttcttccaa tacggtttcg gcttctcgac atagactttc atttacttcg
     6781 ataaccataa agaaggactc ttcttcccct aaccaattca gtacatcaaa atcttcttcg
     6841 gcatcgttac ctacttccag taacccttga tgcataatca gaaacatttt ttcatcatag
     6901 gttaatgctt tagtgagttg ttcctccgtt agctcacctt ttccatattg atcgagacaa
     6961 tctaaaatat gttcaacatt aatttccata cgcttcacct atttctttcc ttcattttgg
     7021 tttgtagaat ttccctgatc tggttgtcgt ttcaagacta aatcatcggc cttaatgaac
     7081 caattgacag tagttccttc tcggaaaaca taattggcga ccgtatccat gattggattg
     7141 accaactcta cctctgcgtc ataggcaaac tcgcgtaagg gtacatttgc tgggatacta
     7201 acttggatca ttcgaccttg cgcagaggat ttcaaattat aagtccgttc ttttacctcg
     7261 ttactaaccg ttccatcctc attttgcttc cgtacttctc ggcgtaatgc ggaaaatcgt
     7321 aatttcccaa acgttagtcc tgaatctaca acaatacctt ctgttaatct catcttcttt
     7381 tcctcctatg ctttcactaa atcatcgaca tacaacacgt agtcggtaaa cccacgattc
     7441 tcaatctcat agcctttggc aaccaaatgg ggattcacgg cttttagtgg ttgatcttga
     7501 tcaaaatcct tttcgccggc ttccgcagct accaccactt caatatcatc ggctcgttgc
     7561 ttactggaat acaaatgata actacgacca attactgtag tattccgccc atatccttca
     7621 gttaaaactt cgccttcgcc ggcatacctt atctttccaa aggtttcggc catatctggc
     7681 acaacaaatt tcaattccat cttcttttcc tcttttcatt tttagttttg gatacaaaaa
     7741 tagcgcgacc acttttttta ggaggcggtc gcgcacgttg gtttttccta tttagttgtt
     7801 tcatcacatc accttagttt cggcgattcc agaaataata cccagcagtc gcaaagatca
     7861 aggtaaaccc aataaacagc agaacattag aatttttctc ccctgtttgt gggaatgtct
     7921 ttactgtagg actagattct tgagagtcac ttggaacagt tggtatttcc ggttgttctg
     7981 gtgcacttgg tgttgtcgga acttttttag gtgttaaagt ttgagttttt tcttttagat
     8041 cctcattgtg tttcccattg atgtttccgt ctttgccata gttaatttct ttgaaggtaa
     8101 aacttgtacc ttctggatat ttacttgtgt ccactttttc cgcaagtacg gttttagtga
     8161 attctttgtc attcacttca taatcaattt tgccagattt ccaaatttct ttggtcgtat
     8221 tatccggaag taatgcatac agaattgttt caaaagcttc ttttgagcca tccagtacat
     8281 catgggtaat cgacacatca tcgaacatat ccaccacatc cccatgagta aaggtttgcg
     8341 aaccatcttc taggtgggct tttgtttgga tggaaacatg gcgttccacc gtacaattga
     8401 cagtttgggc ttgattgttc aagctagcat ctttcgctac tggatcatca cctgcttcat
     8461 agggtttctt gttttcatac acatagttga ataagacgat ggttttatcc aaaacttgtt
     8521 ctgcggttaa tttatgcaaa attttccaag ttcctgtttt ctctttattc gccaacgttg
     8581 cggttgtttc ggcaattacc actggtttgg ctttttcatc tttttcttgg gcagcttttg
     8641 tcgcttccac atcaatggct tgcgctacca cataccagtc ttctttgatt tcatgcaagt
     8701 tatagctcaa tttatcaact agctcggtag aatcaaggtc tgtcaattct ttgcctccgt
     8761 ctttccaagt cccaagagaa gttaagctat cttcttcttc aggtaaatca tagagcatca
     8821 aacgattcaa gctaacagaa aatgctttat tcgtcagttt ctcgtaagga acggtcaccg
     8881 ttttaattgg ttgtttttgt cctttttcgg taatcgtaaa aatgtattca ctcttagtgt
     8941 aatccgcttt attttcttta aaagtagaac ggatttctaa tggcgtaatt ttttgaaatc
     9001 cttctggagc ctctacttct tcaagtaaat aatccccata aggaagattt tcaaacttgc
     9061 catagccatc aaaacctagt tgctcgttat aagctgtaat tgcttcatct tcggcatctg
     9121 taatctcact ggttccttcc aatggcgata ctttaaaggt cagatcattg aagccagttt
     9181 cggcagtccc agcagctgat ccagcaaatt taaagaaatc aaagccaaag cgaatgactt
     9241 gttcttttgc cgtaacatct cgggtaatca cggcattttt ttcgttatca tccacctttt
     9301 tgatggaaac agggtacttt gtttcatcca aggtatatcc ttcaggtgct ttggtttctt
     9361 gccagtaata ctcattaatc gctaagtgtt taacagcaac ttgattcttt tcatctaaag
     9421 tcaaagtcac cgtttcatca gaagctttcg ttcccttcac taactctggt ttaaaagcct
     9481 cactccattt gaccgcttga ccatcttttg cagtaaagag agtatattca gctcctttaa
     9541 actcagcttt accttgagtt tcattaccgg tatctttgtc ttcttttgtc aaagtggttt
     9601 caccagtgat ttcttggttt tgccctttta cgttactggt aacaagagcc acggtttgat
     9661 tgacatagtt caattcgact tttactggtt taaaggtatt cacaaagcca ttactagctt
     9721 tggtttcggt cacataatag gttcccaatt ccaacgcatt ggcaatctct tttggtgttt
     9781 ccgcatgacc tttttcatcc gtggtcattt cttggacaat ttcaccggtt ggactgtctt
     9841 tgcgaatggc aaacgtattt ccggctaatg agtatttgtc gttccaaaga tcagttcctg
     9901 tttccacccc cattttattc aaaatgattt gacctttttc gcgagtattt tttgaagtca
     9961 cagaaatagt ctcaccagat ttaatggttg cggtgattgg tgtagtgtca atcgtataag
    10021 gagctggcac tgatttttca gtaatggtta ctttcgcgcc atgaggaatc tcatccaatg
    10081 tcgcaatgcc ttctttatcg gtggtcacgt cttttgcagg taatgttttt ccaaagtcta
    10141 aatggaaaac ggttcccggt acaacatttc ctgtttcttt atcgactttt ttaatcttca
    10201 aagaaccctc agtttccaca tcaattttga ttgtgtaggt attgggctta tcaattgccc
    10261 ccgccatcaa agtttgttga ccagccattt tataagctac cggtgttcct gtattagctg
    10321 acttctttag ggttaattca ccactctctt ttgaattggc atttggagta atcactagtt
    10381 gattgccgtt cacacgataa tcaatatttg cgatattctc gacaacttca tcaaactccg
    10441 ataaattcaa gccgttcgta tctgtcacag tagtcgattg acctaacacg gttttcactg
    10501 tggagttatg gaagcttggt ttcttttgat aatccgcaat ggcttgattg atttttgact
    10561 caatggctgc gatatttact gcactaccat cagaacgttt aattgagtga agcgtgtagc
    10621 cattaacttc ttgccaaatc atcttctggg ccacaatatg tgtatccaca tccgtaccag
    10681 cttccttcca taaaacggaa actaatttcg ctttctccga catatcaggc aatggatttt
    10741 tctcataacc aggagtgaac tcattgtaaa tcggcacccc tggctcaata cagaaaatat
    10801 cttgcttcac accattgtaa actgcatagt gcggaacggt aacttctgac atgggacgac
    10861 catcgctaaa tgtaccttcg acagcatact ggtgagccaa atccaattcc acagttaccg
    10921 tttgtggatg agtaatttca tcagcaaagg caatcgttgg gccaagcgat ccaccaattg
    10981 tttggcataa tagactcagt gttgcgagca aacgccaatt cttaaatctt ggctttttca
    11041 taaaataaat ctcctttcgt ttttgggtaa taaaaaagac gtccaaattt taattttgaa
    11101 cgtctgattt agagtgatac tatttagttt tctttgtctt ggataggttg tttctctgct
    11161 tataaatttt gattcttcga taatcaaatc caatctctgc catcaattcc tcccaatttt
    11221 tgttcgttct tttcataatt gtatcaactg atggtagttt tggggattga actagttttc
    11281 gaaactttgt cattgataaa tcatcctgta gttttaatcg gattatttca ttttttagtt
    11341 ctaacagcag ttcgaaatca gtaaatcgag atccatcatt tttttcttta aaaagtggac
    11401 gtatatctcc taacatcttt ttaatggtac taagtgaagg aatattcttc cctactgatt
    11461 tttgttcgta cattcgttga gaggttatct tctcgcactt aataaaggct tctactattt
    11521 ccaataattc ttgttctgac attctagccc attttccata cccagtattt tctcgtccta
    11581 tactaatcaa taaattctcc catgatccat attttttgtt aatgaaccac atacttggtg
    11641 cagaatttgg attttctgat agctttttct ggtattgacg gctagatgta attccgtgtt
    11701 tttcgatgaa ttcattaatt gttaaaatca gttttaactc actttgattt ttccaaatga
    11761 tacgtccatc attttgcttc acgataaaaa ctcctaatag ttatttagat agaagcctat
    11821 cacatcaact ttttagtaga aaagtacatg ttttgtcttt tttgctaata gctgaccctt
    11881 tgagaaactt gggttcaaaa gttgctaaat tcggtaaatg ttttggtatt tttcgtatta
    11941 tatttgtcgt caagctgact actaaatttt gccctatttt gacgacactg atagtcaatg
    12001 tcggacaatc taggacaatt tttaagggaa tatgattgaa aaatggtcca aaacagccaa
    12061 aaatggctta aaactgtgat agagaaaaaa gaaaaagccc ttgatataca aggacttttc
    12121 acaaatttca gatttccaag gaagttttcc gcgtttatct tctgatttct ttgatacgag
    12181 cagcttttcc gtgtaatgca cgtaaacaaa accactatat tctattatgt atctaatacc
    12241 ctcaaaacag ctataaatca acaataccga catataataa acactatgtt caatctgaga
    12301 tatatcaaag gtaaacgtat ttttgccccc tttttgcccc tcaaatatcg ctttacaacg
    12361 agaacgtaag ttcgtataat cgttttgagg tgatttttat gatggaagac tttattagaa
    12421 aaaatatcag tgatgaatat gcagactttt atgagcaaag cagcaaaaaa gataaattcc
    12481 agatggatgt ttcaatctta gctatattag ctttttccga aaataaccaa cctgtaattg
    12541 cgaaaaaaga aacagtatta tctgaaggca aaataaaaac tcgatatata ttagaggtag
    12601 aaactaagtt taaaaataga tcggagtaat ggctatgctt tttaatgaga cacaattatg
    12661 gtttaaattt gatccttcga atagatttat caaagatttt tataaggtgt gggattcaga
    12721 agttttcttt ctagcaatcg aagatagctt attaatcaat ctctactatt ctaataagaa
    12781 ctactttaaa atccctgctg caaaaactag aatgaagaaa gatgtatact ttttgtttga
    12841 tatc
//
LOCUS       DAZKMK010000080        12827 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000080 DAZKMK010000000
VERSION     DAZKMK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.082"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..1644
                     /locus_tag="ACG8V1_002185"
     CDS             7..1644
                     /locus_tag="ACG8V1_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330241.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1789..2529)
                     /gene="truA"
                     /locus_tag="ACG8V1_002186"
     CDS             complement(1789..2529)
                     /gene="truA"
                     /locus_tag="ACG8V1_002186"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0330242.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2720..3355)
                     /locus_tag="ACG8V1_002187"
     CDS             complement(2720..3355)
                     /locus_tag="ACG8V1_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0330243.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3502..4722)
                     /gene="sstT"
                     /locus_tag="ACG8V1_002188"
     CDS             complement(3502..4722)
                     /gene="sstT"
                     /locus_tag="ACG8V1_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0330244.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4941..5939
                     /locus_tag="ACG8V1_002189"
     CDS             4941..5939
                     /locus_tag="ACG8V1_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330245.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6135..7970
                     /locus_tag="ACG8V1_002190"
     CDS             6135..7970
                     /locus_tag="ACG8V1_002190"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0330246.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8030..8746)
                     /locus_tag="ACG8V1_002191"
     CDS             complement(8030..8746)
                     /locus_tag="ACG8V1_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0330247.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8973..9515)
                     /locus_tag="ACG8V1_002192"
     CDS             complement(8973..9515)
                     /locus_tag="ACG8V1_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0330248.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9520..9747)
                     /locus_tag="ACG8V1_002193"
     CDS             complement(9520..9747)
                     /locus_tag="ACG8V1_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0330249.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9826..11706)
                     /locus_tag="ACG8V1_002194"
     CDS             complement(9826..11706)
                     /locus_tag="ACG8V1_002194"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0330250.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11895..12509)
                     /locus_tag="ACG8V1_002195"
     CDS             complement(11895..12509)
                     /locus_tag="ACG8V1_002195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0330251.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12651..>12827
                     /locus_tag="ACG8V1_002196"
     CDS             12651..>12827
                     /locus_tag="ACG8V1_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0330252.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         3998 a         2509 c         2035 g         4285 t
ORIGIN      
        1 aaaaaaatga aaaaaaacaa aaaccaaata atagatatca tttttatgtt atttctaagt
       61 tttgcttacg tcatccctct attcatgtct aagggaatct atcatagtgt caatcaggat
      121 acttactttc atttatctcg aatcatagga ctagataatg tatggagttc tccagtaaac
      181 ttcaataact tcgatcatca tggaacaatg atgaatattt tttatccatg gcttactctt
      241 tatccagctt ttttatttta taaaatgatg ggaaatcttg ttttaagtta taatatctac
      301 tattttttta tcactttttt aacaatggtc gtttcttatt tttctatgaa acaaataaaa
      361 aacaatcgtt atatttcgct gttattttcg attatttata cattttccgc ctatcgtgcc
      421 attgacatat tccatcgtgc ttcattggga gaagctgtcg ctttaacatt tttgcctttg
      481 atactaatgg ggtgttacga aatttatatt agagattacc aaaaatggta ctggttgtct
      541 attggcatga cgctagtagt gtatactcat ttactttctg tcgctatggt ttctgtattt
      601 attggaggaa cgttgtttct ttctttttat ttttgggacc aaaaaatcgc tagactctta
      661 agtcttttaa aagctaccgt cctcaccttt tttttaagtg cgggattctt aattcctttt
      721 attcaacaat ctagagcaca agaattgaaa gtcccactgg gaaaagaatt aagtggtatg
      781 gctcctagtg atatgctgac acatatttta aataataact ataacaacta tactattgga
      841 ttattcttat ttctcggatt gattggtgct tttattttta taaaaaaatt aactgctgat
      901 gatttattta ttttctttct tggtgtattt gttctttttt gttcaaccaa tttatttcct
      961 tggcaacttt ttaaccatac accagttaag agtctacaat ttgtctggcg gttaaatggt
     1021 ttttcttctt tatttatagc ctatacgatg agtatagtga tctattatat tttttctact
     1081 aaaaataatt catggaaaat aatggtcttt acgtttcttg cactaatatt gcatctctca
     1141 ggagtaagca attctatcca tgctaacaaa gattcattaa cacttatagc acctgaagac
     1201 gctgttgcta tagctgaaaa ttacaatcat actgattatg caaacaaaga gtcaatctat
     1261 catcctgata tgattaataa tgatcaatat ttactaggta accaggaaat aaatccaaca
     1321 actcatttta tgagtaacaa acttacaatt gaattagata attcgaataa taaaaatact
     1381 gtcttgacga cacctattta tcgttataaa gggcaagtgg cttctattaa tggcaaacta
     1441 gtacaaacaa aattatctaa gtttggcaca actgaattaa ctattcctcc aggtataaat
     1501 aaagtcgtta ttacttatca gtacacaaaa ctagcaattg cttcaagata tttatctatc
     1561 gttactttga ttttattttt actttatcga tttacttttt ctaaacaaaa acaaccgaga
     1621 agagaagtac aacattctca ctagattttt atttaagaaa gactttttag ctgtacacat
     1681 aaacaggcta aaaagtcttt ttttatattt ctttaatttg cttcatacat tatttaacat
     1741 cgatatttat actgtgaaac atgcgtttca tccattatca aagtattttt atcgataata
     1801 aacttcatcc aaaaacagac cttgtgcagg gacggtttct cctgcatgtt gccgcacttt
     1861 gctttcaaat atttcatcga ttgtttctat cggcatggca cctgtgccga tttctagtaa
     1921 tgtccccatg ataatccgca ccattttata taagaatccg tctccgacaa aagtaaaatg
     1981 aagcatttct ccctttcgtt cgatagataa ttcatcaatc gtacgcactg tggatttttt
     2041 tgattttttc aatgcagaaa aaccaatgaa atcatgtgta cccactaatt tttcgcaagc
     2101 tgcattcagc ttgtccatat ccaactcttg agggaaataa aagctgtggt ttcgctcaaa
     2161 tgctgaagga atcacgttgt tccaaacata atagctgtat tttttgccca ccgcattata
     2221 tcttgaatgg aagcgctcag gcatttcttc gattcttttg atgacaatat cccgcggaag
     2281 gtaacgattc ataaagtcga tcatttctgc cagtccaatt tctgtgtttg ttttaaaatt
     2341 tgctacttgt cctcgcgcat gggtgccagc atctgttctt cctgatccaa taatttctat
     2401 ctcttcattt gtcatctgat gcaatacttt ttcgattttt ccttgaatgg ttttatcgtc
     2461 atctccaagt ctttgccatc ctgaatatct ttttccgtca tattcgattg tcaacttgat
     2521 gtttctcata ttttagtggc tcctctgtct tccatttggt cttctctcta cctttttaag
     2581 attgtagcac aaaatatagc tccttgcttc tctaagacag aagctataca agaaaactgt
     2641 ggaaatatat atttcagaaa aacaaaaaac agccaccgga tgaagtatcc gctgactgtt
     2701 caatttgacg gctcaagttt tactgtttga gatgcaagat actcaaccag gcaatgggca
     2761 cataattaaa gaaaaaactg attctttggc aaaaacctat tttattcagt aatggccaag
     2821 aatttataga aggaattctt gccagtccat aaatcgccgc aaaaagcaaa ctgatcacag
     2881 acagtccaag gtataagtgg cttacgtcca tttgcccatt ttttcgatag atcaagaaaa
     2941 gaagaactgg aaacaataaa aaaccactgt aacctaaacc tgatccgata ttatggaccc
     3001 atgtcgagac attccacgaa gattcatggg tattgatact gaacaaacct gtaaagatac
     3061 aatcacctat accatagaac gcgatggctg ctgtaactag ggtagctaac acacgagatg
     3121 tactaacaaa tgcagcatac aatgcaggca atgacaggat gaaaaagatc cctgaaacca
     3181 cagaccaaat aagaaacgct tttcttacag gactggaaac ctctccaaat acactgatca
     3241 cgctcttcat ttgatctaat tcaggataat acaatcctaa tacgtacgga gtcaaaaaat
     3301 cactcagcac gcctaataat aaaaagaaaa aaccaaattt acgtaaaaaa tccatgatac
     3361 gcctccaata acaattacct atgcttttaa ttgttattat aaacgacttt atcttgattt
     3421 aacagaaacc tctatttaca cgaacatcta tataactcat taaaaactcg aatcatttta
     3481 aaaatactaa atatctaacg ttcaacgttc acttaatcca tctaaacaat ctttcaattt
     3541 gattttttcc ccacttcttt tacgcgcgcc tagctctcct attgcagtaa acaacagatc
     3601 acttgacgaa tttaaggcag tttccattga atcctggata acaccgataa taaatccaat
     3661 accgacgatt tgcatcgcga catcattaga gataccaaac aaactacatg ctaatggaat
     3721 caataacagt gaaccgcccg caacacctga agctccacaa gcagctaaag cagacaaaat
     3781 acataaaagg actttcaaaa caaaaggtgc ttcaatccct aaagaatgta cagcagtcaa
     3841 cgtcatcaag gaaatggtaa tcgcggcacc gcccatattg atcgtagcac ctaacgggat
     3901 cgatatggaa taagactctt ttgtcaattt caatctttca gccaacatca tgtttatcgg
     3961 gatatttgcg gcagaacttc gggtaaagaa agcgggaagt gcgctttctt tcaaacaaaa
     4021 gaaaatcaat ggatagggat tttctttaat aaacaaaaac gtgaggaatg ggtacacaac
     4081 taatgcgaca aacagcatcg taccgatcaa aacaagtaaa agctgcagat actctgccaa
     4141 tccagcgatt ccagtcgtag caattgattg ataaaccaat cctaaaatcc ctatcggagc
     4201 aaatgcaata atcacttgta cgacttgtga caacgctata gatatctgat ccaccaactc
     4261 ttttgtcgat tctgaacgtg ctcgaaaagc tagtcccaac aatacagccc agaataagac
     4321 gccaaggtaa ttcgcatcaa taagagattg aacaggattt tgtgtgacat tcgtcaacat
     4381 agtagataaa acactttcca aactttgcgg tgctttttca gatactgttt cttgtaaagt
     4441 aataggaaca gtaaaaattg aacttacaat aactgctaca agtgcagaaa gcaatgttcc
     4501 cactaaatat aagatcaaaa taggcttcac gaatgtctta ttcccaattt tatgcttggc
     4561 aattgacgcc atcgtcaaga aaaaaaccag aataggtgcg attgccttca agcaactgac
     4621 aaacaacgtt cccaatattg aaataaatgt ccatgtgggg aaaaagatac caaacaatat
     4681 tccgactaaa ataccaacaa caatacgttg aactaacgac atatccaaaa acttctctaa
     4741 caaatgacca ccctttcaca attttaacaa tcattgtatc attttatgca aagaaactaa
     4801 aggtataatt ttctaaaaat tctaacaata caaacaacca tttcttgcta attttgtact
     4861 ttttctgtct acacgttgtt ttttgagata ttctaagtta ggatgttatt aatgggcaac
     4921 ttatcaggaa acgttttctt atgtttttgt ccaatttttt tgtgaggtgt ttattcttga
     4981 aaaaaataaa gaaggagagt tatggcttcc tgacaatctt tgtcgcttgt ataatgatgg
     5041 ttttacagcg tatattgatt tcagcagtca ctattcctac atatgcaaca aaccctggtc
     5101 gattctacat ctatacattt ttacaaactt ccgttgttat tggtgcaaat ttgatcccac
     5161 tttacatggg ttaccaatat caaaaaatcg cccgtttaaa agttttacat tatttaagtc
     5221 gttttgcctt tatattccta atagccacac tagtttgcaa tatctttttt tacgtacaag
     5281 ctggcagctt gaatattcgt gattattggc ttatcctaac acctatcagt cagaattact
     5341 ttacttatgc tgtttcttgt actttgcttt tttgtagtat tccttataca gttggatttt
     5401 taaataaatt atcgaaaaca acaattaaat atctactatt atttttaaca attggattag
     5461 ttggctgctc tactctattt aataaagacc cgtttgcatt acagaatgga cagagtattt
     5521 tatggatttt ttatttattt ttaatgggtt atggtttgaa agaatggaac tggaaagaaa
     5581 aggtccgcta taagcttcct caagtattta tcagcttgat tgttttattc ggtttgatta
     5641 ttttgatgac acaaatttca ctcatcatca gaggaaatac agatacagcg ctgaggtttt
     5701 ctaaaccata ttctctcttc gcaatgtatt atactttctc ttcgttttta tttttagaaa
     5761 tgctatctga taaattaggt ataaaaggaa gaggaaattt tcttgctgta tttttgattg
     5821 tcactcaagt tatatgtaat ttccctttag tttcttatcg cgtatctaca tttatgaaac
     5881 gtgaatttcc aaactcaggg aaggcatggc tgataaatat cgggcagttt tttatttgat
     5941 atttattagc agtgtccctc ctagttatct ttttatttta tatacaaaaa atccgtttca
     6001 ttcatcgttt catcgaatac ttcgcttttg attcgattga acaattaact cagcgttgga
     6061 aaaaaagaat tcattggctt tttgttcata aaagttattt tttagttgca gcattttttt
     6121 actgctttac cttcgtgcag attttcgttt tagaaccaaa agaaggttgg aaagaaacta
     6181 tccaggttgc cttaaaaata tttactcagc gacaagcacc catgatcctg acaatcatca
     6241 ttattatggg atttttcttc ttactattgt tgatttccaa tcgtttttgg tacgccttga
     6301 ctgctacctt gatcattaac ttactgctca ctatttcgac agtaatcaaa atggaaatgc
     6361 gggaagagcc tgtctttcct tcagatttaa aaatgctgac cggattgtca gaactattat
     6421 cgatggttag cccagtctta ttaatcgtgg gcggattgat ccttctccta ttattgatta
     6481 ctagtattat tgtacaacgt agactacaaa aacagtattc cttgaagatt cattggaaac
     6541 gtcgatttat cagtatcgct gtattattag gatgtttttc gggtgtcttt ttcattaatc
     6601 acaaaaattc gccttctttc ttgctgttta atttatttaa agtaaacaag actttcttta
     6661 accagcaagg cgccgtaaaa gataacggac cagtcattca attcctaaac aatatcgaca
     6721 ttaaaatcat ggaagaacct gctggctatt cgaaagaaaa aattaatgga ataatgaaaa
     6781 agtacaacac aaaggcagat gaaatcaacc aaacaagaag tgactggtta aacaatcaga
     6841 cgatcgttct caatttaagt gaaagcttca gtgatcctgc acgtgtccct aatctgacag
     6901 tacctacaga ccctattcct tacatcaaaa gcattataac agaaggaaca ggaggattga
     6961 tgttatccgt cggttatggt ggcggaacag ctaacatgga atgggaagga ctgacaggct
     7021 tgagtatcag taacctgtct gcttcgttag ttacacctta tacccagcta gtggagcgtc
     7081 agaaaataag tccaaatatt accgatctat tcgatgaaaa aatagccatc catcctttta
     7141 cagctgctct ttatcgcaga aaacaagtgt ttgataaatt tggttttgac aggttctatt
     7201 atttagacag tccggataaa ttaacttata cggataaaat agaacaaagc ccacgcatat
     7261 cggatgattc agcatacaaa gaaaccttga aagctttaca aagcaatacg gaaacgtcac
     7321 aattcatcca attatccact atgcaaaacc atatgcctta tgatgattac tatagtgaat
     7381 tgaactacac tgctgaagga actgctgtca ttcctaatag aagaaaagaa ttacaaactt
     7441 atatgcaagg ccttcactat accgatacag cagtaaaaaa attcatccaa gaaatcgaca
     7501 aaatcgataa accgattacc tttgtttttt atggtgacca tttacccgca atctatagtg
     7561 ggaatgattc gaaaaaatat ggattagaac agcaccagac agattatttc atttataaca
     7621 acaagtatag tcgtgaacaa gcagctacgt taaataaaga agttgttgct cctaataact
     7681 ttggtgctat ggctcttgag catgcgaata tcaaagtaac tccttactat gcgttgttga
     7741 cagaagtatc taatcatcta cctgcattaa caattgatcc tacagaaagc ttgtctaatc
     7801 gttttaatgg aaaacaggtc tttgttacag aggatagtca aattatccaa gaaaaaaatt
     7861 taactgagaa acaaaaagaa attctagaag attatcgtct gatacaatat gatcttgttg
     7921 caggtaacca gtacagtgca acatgggcga cacaaaaaat aaaagactaa caaataaact
     7981 gagcaagggg ttgtgataaa agacatgtca caaccccttg tttatctcat taatttgctt
     8041 gtccataaac gatttgatta aagccatttt tcaattcatt attcgctgtc tcgttgaagt
     8101 aaagataatt atcgggattc ccctgatttt gttgtgtcac caatactttt ggctgatttt
     8161 gctgaaaccc gaatagatag acgtgctttt tcagatatgg ttctgtctct gcatcggaat
     8221 agacggctgt gatttgatag tctgcttgac ctgtcccact ttctgaccat tctgcagaaa
     8281 caggcactcc accaatagcc atcttccact ccccgttcaa aatagcttga gggactttaa
     8341 gaccatataa gtccacactc atttgattcg tataactttt gtattgttgt cccattgttt
     8401 ggccccattt ggtcataaat gcctctaatt gtgcggattt gtctgcattc cacagtgttt
     8461 gttcaacttg ttcgctgctt tctgaagagg aagatgaagc ctctacagaa gaacttgaag
     8521 tagatgtttg agttgtttca gtcgttttgg aagactctga cgaagtagag gtatctgctg
     8581 tttctgtact tgctgcagtt gacttgctct ctgtgctagt tgttgtagta gttgcaactg
     8641 ttgctttcgc tgtttctttt gttgattctt ttttcgattc attcccgttg gtacaaccag
     8701 ctaacaaaaa agctagacca attgttacta aaatcctttt tttcaattaa aaaactcctt
     8761 tcgattactc ccacttcttt attctatcaa ataagcaatg tgattttatt gatttttcgt
     8821 attttctcat caatctttca aatcttctaa aaactgagaa gcacccgctg atgttgtctg
     8881 taatacatca gcgggtgctt cttttatttt aatcttcgtg attttccaat ttacctcacg
     8941 ctttatattt gtatttgaag aaacatagac ttttagtcat catcgtcttc ctcatccaaa
     9001 tcttgtcgga cagtttttcc ttggagtctt tgtacttggt agatgatttc tttattgaaa
     9061 cttaaaagtt caacaagcaa ttgttgcaag ccatattttt cttctttttc agctaatttg
     9121 attgcccgtg tgataaagac attttggaaa tcaaaatctt ttgcaaagtt ttccaacatt
     9181 gttgaggctg gtaaatattt gttttgacca tgttcgtaaa tttttccata acgcatgact
     9241 tcttctaaag tagacggaac gatttcattt tcaatcacta actcatgaga gattttattg
     9301 aaccatctcg cttcttgatc agcatattct gaaaaaaggg tacttaattc ttgcgaagat
     9361 gtacccaaaa catacaattg tgcttggatc aatttcattt cttctaattt aaaatttgct
     9421 aatacatgcc ccaccatggc gccagcagtt ggtttatgat ggtcgatatc tgcttgtttt
     9481 gtttcttgaa caaatttttc ttccactata ttcattgatt tacgcctctt ttacttttgc
     9541 tgattggact tcataaccta atttttccac aactgcagct aattgttcag ggtggacttc
     9601 atctggatca aagtttgctt tgattttgct cgcattaaac aaaactttcg tattgcttac
     9661 cccaggctgt tgagaaaccg catgttcgat tttttgcata cagcttggac aagctagtgt
     9721 atctaattgc ataataattt ttttcatcgt agttcttcct tcctttttct tgatagtctt
     9781 attctagatt acagcttcca taaaaaaatt gattcttatc aagttttacg atttttcttc
     9841 tttgctttcg tgtccaaaat acaataaacg catcgcgtta aagataacga tcaagatact
     9901 agcttcgtga acgaacatcc cactagccat gtaaataaac ccggcaaata agccgttgag
     9961 taaaaataag acgacaccaa tggcaatcac tacattttca cgggtattta aaacagtttt
    10021 cttagccaag cggtaggcat gaaccaacga ttcaaaatta gattgcatca agacaacgtc
    10081 actcgtttcg atagcaacat ccgttcctga tcccatagca atcccgatat cagccgcagc
    10141 caaagaggga gaatcattga ttccatctcc aataaaagca acgcgcaatc cttgttcttg
    10201 gtattttttc acaaattgaa ccttttcgtc tggtaataat tcagcatgga cttcatcaat
    10261 gcctaacata tcagctacat aatcagcagt catctgatta tcacctgtca gcatcactaa
    10321 ctgttttgct cctgcttgtt tcaacttagc taattgttca gcgacttctg gtcgaatcac
    10381 atctgacaca cctatgattt gagtgatttt tttgtcgatt gctacaataa tgaccgagct
    10441 gcctgtttgt tgtaattgat gtaaatcttg tacttgcttt ggtgtgagag aaagtttgtg
    10501 agcggcaatc aatttggtgt tgcccgcaca aactgtatga ccatcaatct cagctgtgat
    10561 tccttgtccc ttgattgtct gattatcttt gactgctaat tgttgaaaat caatgtcttt
    10621 ctcctgagca taatggatca cggcgcgccc cagaggatga tcggataacg tttctaatcg
    10681 agccactaaa gctaagctgt ccccttcatc ctctgtatag ttcttgatag tggatacagc
    10741 agtagttcct ttggtcaatg taccagtttt atcaaagaca aaagtatcga ttttggaaaa
    10801 agtatccatt gcttcaccgc ctttgactaa gatgccccgt ttcgctccat taccgattcc
    10861 tgctacattc gaaacaggcg caccaatgac taaagcgcca ggacaaccaa gaaccaaaac
    10921 agtgattgct aaacgaaaat cacgactaat cagaccaaca accaacgcaa taagtaaaac
    10981 ggttggagta taatactgag aaaatcgatc aatgaatttt tccgcatggg atttggtatc
    11041 ttgtgcttct tctactaatt cgataatttt ggcaaaggtc gtatcatctc cgaccttagt
    11101 tgcttcaatc gtcacgtagc catctgctaa aatcgttcca gagaagactt catctccact
    11161 gattttgacg acttgtcgtg attcaccagt cactgcggct tcatccgcat accctttccc
    11221 gtcaatgact cgtccgtcca caggaatagc tccaccagtt tttactaaga cgatatcgcc
    11281 ttcttccacg tcatcgattt ccacttcttc agtcgttcca tcttcttgga caacactagc
    11341 agtagatggg gccatctcgg tcaaagcctt gattgactcc cgcgttttgg tcaatgtttt
    11401 ttgttccaga tagctaccaa acaaaaacaa gaatgtaacg atagctgact ccttgtattc
    11461 tccgatcata aaggcaccaa gcacagcaat agtaacgagt aaatcaattg agataacttt
    11521 gactttcact gcctgatacg catggatcat aataggtatg accgcaataa tagaagctat
    11581 ggctagagca atgttataga acaagacaaa accagtaaat tttccgataa aaccaaaaat
    11641 aatcaacaca gctgataagg cggtgatatg atttcgatgc tcttggatcc acttttgtaa
    11701 tttcatcttt ctccctcttt tcctttcatt cttacgagac taagtataga tacttatcca
    11761 tcataaaaaa ttgaccacta tcaagtttag tcaaaaaggc ggttttcttt tggtagaata
    11821 cactacaaaa aatagttagc aagaagactc actaaaagtg atcttgccaa ctattgtcgt
    11881 ttctatcgaa attttcattg aatgattttc ctgtacagca tacatcggag atctatttct
    11941 ttatctaaga tttttatctt gctaattttt tcctttagtc tttcaaatcc acgtcgttca
    12001 tagaattgat acgtacagcc actatctgta aaaaggaata tttcttttcc tttctctctt
    12061 ttttcaaatt ctgttagcaa cttggtaccg atgccttttg ttttcaaatc aggatttgca
    12121 gctaaaaaaa tgatctctcc atctggcgaa tgcttcttta aatatgctgc aagcatttgt
    12181 tcatttgctg tctcgtaagc tcctttactt tctttagcaa atatattttg taaaaaatcg
    12241 aatatcttaa catatatttg ctgtccaaaa gaacgatgtt tctttttttc tccttttatc
    12301 tccgccagca aaacacctgc caactcgtcc tctgcataca aagcaagaat ttgagttgct
    12361 cgattcaact ccatatacaa aaaatatctt ccgtaaagat tcagcagaag ttcattttcc
    12421 atatatctct caaagttcat tccttcaaca gcaaaataga tagctttttt gtaatctttc
    12481 ttttctaagg gtttaaaagt tagtttcatc atttcgcctc ctcaacttca atgattgtgt
    12541 tatacttaca ttataataga cacagtgtct actatctata atcaatcatc tataagtttg
    12601 tcgattaagc gacagctttg ctttgatatg tcggataagg agataggtaa atgaaaaaga
    12661 cagatagacg cgtcaaaaaa acagaaaaag ctttggctga aacactatcc acattattag
    12721 taaataaaaa gattcaagca atcactatac gcgaactaac agaaacggca gatgtacatc
    12781 gttcgacttt ttatacacat tataaggata tctatgatct atatgat
//
LOCUS       DAZKMK010000081        12747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000081 DAZKMK010000000
VERSION     DAZKMK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.083"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8V1_002197"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8V1_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0330253.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8V1_002198"
     CDS             complement(323..892)
                     /locus_tag="ACG8V1_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0330254.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8V1_002199"
     CDS             1078..1980
                     /locus_tag="ACG8V1_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0330255.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8V1_002200"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8V1_002200"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0330256.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8V1_002201"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8V1_002201"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0330257.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8V1_002202"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8V1_002202"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0330258.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG8V1_002203"
     CDS             8554..8739
                     /locus_tag="ACG8V1_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0330259.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG8V1_002204"
     CDS             complement(8801..9997)
                     /locus_tag="ACG8V1_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0330260.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG8V1_002205"
     CDS             complement(10167..11603)
                     /locus_tag="ACG8V1_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0330261.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG8V1_002206"
     CDS             11896..12705
                     /locus_tag="ACG8V1_002206"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0330262.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAZKMK010000082        12658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000082 DAZKMK010000000
VERSION     DAZKMK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.084"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1958
                     /locus_tag="ACG8V1_002207"
     CDS             75..1958
                     /locus_tag="ACG8V1_002207"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0330263.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2033..2317)
                     /locus_tag="ACG8V1_002208"
     CDS             complement(2033..2317)
                     /locus_tag="ACG8V1_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0330264.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2307..3041)
                     /locus_tag="ACG8V1_002209"
     CDS             complement(2307..3041)
                     /locus_tag="ACG8V1_002209"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0330265.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3170..3793
                     /locus_tag="ACG8V1_002210"
     CDS             3170..3793
                     /locus_tag="ACG8V1_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0330266.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3985..5106
                     /locus_tag="ACG8V1_002211"
     CDS             3985..5106
                     /locus_tag="ACG8V1_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0330267.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5113..8241
                     /locus_tag="ACG8V1_002212"
     CDS             5113..8241
                     /locus_tag="ACG8V1_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0330268.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8260..11454
                     /locus_tag="ACG8V1_002213"
     CDS             8260..11454
                     /locus_tag="ACG8V1_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0330269.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11600..12307)
                     /locus_tag="ACG8V1_002214"
     CDS             complement(11600..12307)
                     /locus_tag="ACG8V1_002214"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0330270.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4310 a         2222 c         2654 g         3472 t
ORIGIN      
        1 gattctactt gctattttca cttaataccc gtatattacg aatgaaaaaa gtttatgaaa
       61 agttggaata ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt
      121 ttgatgggct ccgttcatta gctgtggtgg gtgttatctt ctatcacctt ctgccaacta
      181 gtatgaaagg cggctatttg ggcgttccga tttttttcgt cgtatctggt tatttgatca
      241 cggatctgct gcggcaggaa tgggaacaaa atggaaaaat caatatttgg caattttatg
      301 tcagaagaat gaagagactc tatccggcac ttgtttttat gcttgtgaca gcttcggctt
      361 atatcacttt gtttcagcga gggctgttaa acaatcttcg aggagttgtg gtcagcagct
      421 tactgtatgt caacaactgg tggcaaatca ataacggttt atcttatttc gatcgatttg
      481 ctaatgagtc accgtttacg catatctggt ctttagcagt agaaggtcaa aactatttgg
      541 tttggccgat cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa tggatctttt
      601 atactgtgtt aggtacatcc ttcctctccg ccatcttgat gatgatcttg tatacaccag
      661 gaggcgatcc aacccgtgtt tattacggta ccgacactcg tctgttttct atatggttgg
      721 gaagtgcgct tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag
      781 cgaaaaatat tctgaatatc gctggaggaa tctcgctttt ggcactgatt ctttgcttct
      841 tctttttaga tgatcattac aatttcatct attacggagg aatgctccta gtgagtctgt
      901 tatgcgtagt gctagttgca gtgacagcac accccggagc aagtttgaat cgatggctga
      961 caaatccagt atttacttgg attggcaaac gaagctatgg catttatctg tatcagtttc
     1021 cagttatgat tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata
     1081 ctttagtgga aatcacattg attctaatca tcagtgaact ttcttatcga tatgtggagc
     1141 gtccgctagc acgttttgat tatggtcgta cattccaagt gatgaaagga tggttcagca
     1201 aaccgatcat cagtaagagc aaaccatggg ttgttccagg cacattactt gtagcaattg
     1261 cactcgttgg tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc
     1321 aggcaaaaat cgctgaaagt aaaaaattag cagaagaaac cagaaacaat tcagaaaaca
     1381 ccactagttc ggttgaccaa gcgatccttg ataagtacga gttgtccaag gcacaaggga
     1441 ataaggcaca aaaattagaa ctaacggcat ttggtgattc agttatgttg gatgctgcaa
     1501 cggatctaca agaactttat ccaaaagtag tggtagatgg agatgtcgga cgacaattat
     1561 atgccagtgt accgtacatt gaaaaattaa aggaacaaga tttgttgaaa gacactgttc
     1621 tgattggact aggaacgaat ggctcattct cggaagctca gtttgatagt gtgatggatg
     1681 caatcggtga tcgaaaagta tactgggtca atgtccgagt accgacgaag cgctggcaaa
     1741 atgacgtgaa tgcgatgctt gaacagatga cgaaaaaata cgacaatatg acgctgattg
     1801 attggtacag ctacagtaat gaccatagtg actggtttta tgatgatcaa gtccatccaa
     1861 atcctgaagg gatgacacat tacattcact tagttagcga agccttgcta ggaggaagtt
     1921 caagtacgaa cagcagtgag acaaacgtga cagaatgata gctacaaaaa acttcaggca
     1981 aaaacatttg tttttgcctg aagttttttt atatttaccg tgtttttaac attcatatag
     2041 attcaggaag caaaacggtt gagtgtgtct gtaaataacg ttctttttgt tttcgggtga
     2101 gttttttgaa tgcagcttct gcttttgttg cttcgcttct tgaagaaaat acttctgcat
     2161 ggatcatctt aacaggtctt cttttttgaa gacgagtata tttagcacca gtaccattgt
     2221 tatgttcctc taatcgtcta gaaagatcgg ttgtatatcc accataaaat gtctgatctt
     2281 tacagaataa tacgtaaaaa taatgttcat ttgccataga gcatctcacg aatctctgca
     2341 gtgtattctc catccatatt gtaagtgatc aatggtggag cgactttgaa tccgtctaat
     2401 ttgccttctt tgattccttc aactaacaaa atatttgcct cttttccagg ttttgggtaa
     2461 acaaattgga tccttttagg tgcaattcga tagtcttcca tgacctgaat gatttctaag
     2521 aaccgatcag gacgatggac catagtaaag cgtccatttg tttttagtag cttacttgaa
     2581 atatcgatca cttcttctaa cgtcgtatga atctcatgac gggcgattgc caggtgttta
     2641 ttagggttcg ttttatttgt tggcagccct ttgaaataag gtggattaca gacgactaag
     2701 tcacaagaat tatgtcgaac gacagataag ctgtctttca gatcgatggt gtggacagtc
     2761 atttgttcat ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt
     2821 tgaagttcga tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg
     2881 ccatttcctg cacaaaggtc aacaatcttc ccttttttag gtacacggga aaagtgagca
     2941 agcagcacag catctaaaga aaaagaaaac actttggaac tttggataat cttgatatct
     3001 tctgcgtata attggtcgat tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa
     3061 aaaaatagtc gtctgtatta tactattttt attaaaaaga accaaggtgg cttgtcaaac
     3121 ctctattttt tatgcatact atgaccataa gaagggaaag gaagagacta tgttttatcg
     3181 atttatgcgc ggggtagttc gagtactatt agccttagtt aatggaaatg cccgctatga
     3241 gaataaagaa gttttaccgc aagatgagaa ttacatacta gttgctcccc atcgtacttg
     3301 gtgggaccca ttatatctgg cggtagctgc agcacccaag gaattcagct ttatggcaaa
     3361 agaagaactg ttcaaaaatc cagttctgcg atttatatta actcgatcca atgcttttcc
     3421 agttaaaaga gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc
     3481 gacaaatttg agtttgatca tgtttcccag tgggacaaga catgctacag aacttaaagg
     3541 cggaatggca ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg
     3601 tccgttgaca cttggggatc tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc
     3661 aatcgactta tcagatatca agaaaacgga taaagaaggt atggcggaga tcgaacgacg
     3721 aacacaagca gcttttgaca aattggacaa agaagtgaac cctgatttca aatatgaagt
     3781 caaaaaaaaa taaaatgata agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt
     3841 cagttcagac tttttcaatg aaaacgaggg agagacgtca agtctgccct ctttttgcgt
     3901 tccaatatgt aaaatagaag acaggtgtca tgtaatggat caaagtgtta taataattag
     3961 aaggacattt tttgaggtga ttgaatgcgt tttttacata cagccgattg gcatatcgga
     4021 aaaaaactac aaggttatga cttgattgaa gaccagcgtc atgtactatc ttctatacta
     4081 gatatcgcga aagaagaaaa agtagaggca atcgtgattg ccggagactt atatgatagg
     4141 agtgtcccag ctgttgatgc cgtagaatta tacaatgaat tgatggtcga ttggaattta
     4201 aaggaaaaga tcccgatttt tgctatatca ggaaatcacg atagcagtac gagactttca
     4261 gcgggtacgc catggttcag ttattcgaac tattttttgc gaacacagct agcagaagca
     4321 tttgaaccag tcgagtttaa aaacgtacaa tttttctttc ttccttactt tgaaccaatt
     4381 gcagcgagat tatattttga agatgacaat atccgaacga tcaagcaagc ggtagccaag
     4441 gtgatagata aaatgcagga gctatttgat tcagaaaaaa aacaagtatt gattagccat
     4501 ttttttgtaa tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt aggcgggttg
     4561 gattcgatcc ctggtgaact acttacaccg tttgattatg tagcactagg acatttgcat
     4621 aatcaagctg ctttgagaca ggataatgct cgctatagcg gttccccatt gaaattctct
     4681 ttatctgaga tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac aggcctggag
     4741 ttgaatttta gggagatcga acccctgcat gatattgaag aaataacagc gagtttcaaa
     4801 gaattgctgg atcctgcctt ttatcagtcc gtcaatcggg agaattacat ctatatctat
     4861 ctcactgata gaacgatcat cccgaatatg atgaaccagc ttcggcaaat atatcctcgt
     4921 attttgggga ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta
     4981 ctagaaatga aagatcctca aaaattagca gaagcatttt ttagtgaaat gacggaagag
     5041 cagctaactg caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat catggaaatg
     5101 gaatgatcaa gaatgaaacc gaaaaaatta caacttaaaa attttggtcc ttttatcaat
     5161 gagaccgtag atttttcacg tttgacagaa gcaccgcttt ttttgatcag cgggaagaca
     5221 ggagctggca aaacgacgat ttttgatggg atcaccttcg cactttttgg tgaaacttcc
     5281 ggaagacttc gctctggaaa agagatgcgt tctttgtttg ctacaccaga cgaagaaaca
     5341 agcgtgactt tttcttttga acatcagcag atgagctatg aaatcgaacg aaaaccagaa
     5401 caagttttag caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa agtaacgttg
     5461 accatttttg atgggaaagg aaaagaaata aaacaaatca caaaacgtac agaagcagat
     5521 cagttgatca gagaactgat gaatctcgat gcgaaacagt tttctcaaat cgttttacta
     5581 ccccaaggag aattccgtaa ttttttggtt tcatcaagca gtgataaaga gactgtttta
     5641 cgtcatttat tcggtactca atttttccaa cagttcaatg agcgattaaa agaaaaagca
     5701 aaacaacagc aacaaaaatt ggatcattta gagcaagagc tgcatcttct gcaaaaaagg
     5761 tttgttttag tcaaagaaga ggaaccagca gcagggtttg aagccgtttt agcccaatgg
     5821 acaaaccatc aggcaatact cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa
     5881 cttcaggaaa aacaaaagca actagaaaat tcctactatc gttttgaaag cttgaaaaaa
     5941 agttatgaag aaaaaacgat attaataaac aagcaaaagg aattagctgc tcaaaaagac
     6001 gaaatagagg aaaagaaacg atggattggg tattatgaat ttacgcaacg attagtcgaa
     6061 ccaatccagc gcctacaaga aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt
     6121 gagcaaacaa agaaactagc gatgactacg cagaattatg aagagtggca aaaaaatgaa
     6181 gcaaaccgcg tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa
     6241 cagatgatgg tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc agaacaatta
     6301 acaatttcat tggataaaga agaattgaat gccgagaagc tgtatcaaaa tctcgctatc
     6361 tatcaggaaa gaactcgaat tcttcaagaa aatttactgg atcaggaaaa actgcaagaa
     6421 gatcgtcttg cttttggaca gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa
     6481 caaaaagcaa aagacactgg gtcgctcgca cagataagag aagaactcaa aaaagcaact
     6541 gaagaaataa caaaaaatag tcagctattg aaagaacaga cagaacaagc agaacagctg
     6601 aaaagccaat gggcaaagca gcaaattgct cgtcttcagc tttttttgcg tccaggggag
     6661 ccatgtcttg tttgtggatc aacggaacac cccatacaac aattaaaaca cgaacagcca
     6721 aataaggaag atatcttaaa agtagaacaa caactggcag aagctgagtc tgctgtagaa
     6781 agaactcagc aaatcattgc caaaagtgaa gcacatgaaa ataatcttca acaacaatta
     6841 tcagatttag aacaaaaaat catggcaacg aatatacaat tggctgaaca agaagaacac
     6901 ttgcaacagg ctttgaaaaa ggcatttcct gagataacag aacaaaaaac aaaaacaact
     6961 cttgtagtaa tcgaacaatc tttagataga cgaaaaagca agatggccaa tgcaagaata
     7021 gagctggaag aaataagaca acaagcagaa aagctcgaac aacaattagc tgtctacaaa
     7081 cagcagcaga cagaaaaaaa taatcagctc cagcaactgc aaggagaaat caaaagttta
     7141 gaacaacaat tgcaagatac ggatagtaac tgtttagaag aggagatacg acgacttgaa
     7201 aaacagttag aagaagactc gaatcaacta gctgttgagc agaaagcagg agtgcaattg
     7261 ttgcaagatc ttcatacgct tcggacacag cttgatcttc aacatgaaca aatagaaaaa
     7321 atagatgaaa aaatagaaaa ggaacaagaa agaatagcta aaaagttaga aagccagtcg
     7381 tatttttcaa caattgaaga aatctgtgca tttgcacaac agcaagcaca atataaagaa
     7441 aaccaacgta cagttacttc ttatcatgaa gagcaattag tgacagctga ccggctgcgt
     7501 caattagaaa atatccagct aactgagaca ttcccagata cagaagagat cagccaacag
     7561 ttagaagaaa taagacgtat gatcgaaata aagcaagaaa agatatacgg tttacaagaa
     7621 caaaaaatta gtaatcaaaa aatctacgat gaatgtcttt cgatttatca cacaagccag
     7681 acgaaattag atgagcttac gcaattacag cagctttcgc aaacattcaa cggagaaaat
     7741 cctaagaaga ctagtttgga acggtatgtg cttcaggttt atcttcaaga agtgttacaa
     7801 gtagcaaatg atcatctcca acgtttaacg aaaaatcgtt atcaatttga gctagcggat
     7861 accattggca gttatcgagg gaaaaccgga ttagaaatca atatctatga tgatcaggca
     7921 ggtatgtctc gaagtgcgca taccctgtct ggcggtgaga gttttattgc agcactttct
     7981 ttagcattag ctcttgccga tgtgatccag acacaagctg gtggtgtagc aatcgatgca
     8041 ttgtttattg atgaaggatt tgggtcatta gatgaagaag ctttggaaat ggcaatggaa
     8101 gctttagaaa cgatcgaaag tgaaggaaga atgatcggca tcattagtca tgtaagagaa
     8161 ttgaaagaac gagtattaca acaaataaga attgatacag aaggtagcgg acaaagcaaa
     8221 atacgctatc aacttggata aaaagggaga ttggtaacta tgaaatggag tattccagaa
     8281 aaagtcattg cacgtggacg tcaatacttg caagaaggcc gagtgttatc ggttgtccct
     8341 gaccaagaag aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta tatggttgat
     8401 ttggatggta cagcaaaaga gatagattac tgccaatgtc cctattggga ggaacataaa
     8461 tattgtaagc atacagttgc tgtggaactt tatttacggt cgaaaaatct atctcgtatc
     8521 atgaaaaaag accagccaat gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt
     8581 gcaaaaggtt tttctcgtct aaaaacacaa aacaatcctc aagcggtgca acctttgatt
     8641 attgaatacc aagtagaaac gattgagact aatcagtatc acccagagct ttctattttg
     8701 gcactttact tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac
     8761 atctatgatt ttttacaagc ttacacagaa aaagaaactt acactgttaa taaacagtac
     8821 cagtttcgtc tagataaacg ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt
     8881 gcaggaagtg ctcagacgca tcaattatta ggtacgaacg gcttacaagt caaaggaaaa
     8941 ctagataaaa gatatctgct tctcccagta gaacaagccc gcttcctgtt agagcagatg
     9001 aatcagacta cacgtttgca tctgcagata aatgaacaaa acgatcaagg aatacaattt
     9061 tccgatcaaa acaaaccttt atcattcaaa gtggaaaaat ttggagaaga ttatttgctg
     9121 caagcattga atgattttga ttttttcttt atgcattatc agtggggagt tatccaaggg
     9181 acattttatt ctctgacgaa atatcaacaa acggtttatc aaacttggaa acaattgatt
     9241 cgccgattgg agaaaccaga agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa
     9301 gagattatcc ctctgctgtc agagatcggc gacgtagcta ttgcccctgc agtagaagag
     9361 gaagtagcag atatccccgt cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc
     9421 caagctcgag tcgatttcgt ctatggtgaa gtcatctatt cgactgaccc gaagcatgag
     9481 gtctcgacag aacaggcttt tcaggtgtta cgtgacacag tagaagagaa acgagtactt
     9541 gatctattca gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg tccattgcca
     9601 agcggggaag aactatatgc ctttttccgc acagaactat ctgtcttccg tcagtttggt
     9661 gaagtcagac ttggaagaaa attgcgagag ttgtatttag atgcgcataa acatcaaccg
     9721 aaaatcgaag taaccgagac cggttcatgg ctcgatattc gattcgatgt gacagggata
     9781 gaggagcaag aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc tttttataca
     9841 ttagaaaatg gtcaggttct ttcttttgat tcagaagaat ttcaacaaac aagccaagtg
     9901 ttgcaacaat tacgggaatc gatacgtaca gaagaaggaa cgatccatgt acctaaaaat
     9961 caaggattga tcattcaaaa tcaattggaa aaaagcaatg ctacttttag tgaatcgttc
    10021 caaacgatgg tacaagatct cattcatcca gaaagatacc aggcacagct tcccaaagga
    10081 ttgaatgcaa cgatgagaga ttatcaaaag caaggttttc gatggttgaa gatgttaggt
    10141 cattatcagt ttggcggtat tttagccgat gaaatgggac ttgggaaaac actacagaca
    10201 attgcctttt tactttcgga aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg
    10261 cctgcgagct taatttacaa ctggcaggca gaggtgagaa aattcgcccc ttctttaagt
    10321 atccaagtaa tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa agacacagat
    10381 atccgagtga cttcttatgc cagcttaaga caggatctag ctaattatca gtcgcaaaaa
    10441 attgattatt tgattcttga tgaggcacaa atggtcaaaa atagcagtac gaaaactgca
    10501 caagcattga gagaattagc tgtacctcag cggtttgcac tgagtgggac accaatcgag
    10561 aataatttag aagaattgtg gtcattgttt gcaacgatta tgccaggatt tttcccgact
    10621 aaaacaaaat tcagggaact tgccgcagaa gaaattgcgc aaatgattcg accgtttatt
    10681 ttacggcgag ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta
    10741 tattctgcac ttacagaaga acaaaaaact gtttatttag cttacttgcg tcaaatgcgt
    10801 gaagaaatca catcaatgga ttctgaagca ttcaagaaaa accggatcgg gatacttgct
    10861 ggtctgacaa gactgcgaca aatttgctgc gatcctcgtc tgtttattga agattaccaa
    10921 ggcggttccg gcaagctcga acaagtaaaa gatctactgg tagctgccaa agagaacaaa
    10981 cgaagagttt tgctgttttc tcaatttaca gggatgctga ctatcttgca agaagagtta
    11041 gccgagctag ggatctcgac attttattta agaggcagta cgaaaccaca agatcggtta
    11101 agtatggtag acgcatttaa tgctggcgaa aaagatgttt ttctgatttc cttaaaggct
    11161 gggggaactg gattgaatct cacgggagca gatacggtca tcttgtatga cctttggtgg
    11221 aatcctgcca ttgaagaaca agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg
    11281 gtagaagtgt ggcgtatgat ctctgaagga acgattgagg aacgcatgga ttcactccag
    11341 caagaaaaac gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa
    11401 ctgactgaag aagacatccg catgatttta agtgtaggag aaatcgagga gtaagagact
    11461 cgaaaaagcg ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg tttatttgat
    11521 ttaaaaaata gcaaggaggc tgggattcca ttttatccca gcttccttgc tatcattttt
    11581 ttatttctat ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac agctgatcaa
    11641 aagaagaaat ctcgatatcg caaattggag tctgttcaat tttgcgttct cgatggttga
    11701 accaaagcgc ttgccagtta gcactttttg cacctagaac atcattgtcg taattatcgc
    11761 caacgtataa ggtctcttct ggaagcatat gaaattcttt ctcagccagc tgaaaaatct
    11821 ctttttctgg tttttggaaa cctgttgctt gagaaatgag catattgcgg gaaggaatcc
    11881 aattgttcaa ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga
    11941 taattcctaa tgggacattc ttttccttca agaaatcgag ggtttttttg acctcctcat
    12001 gcaagcagat attatcaagt tcttcctcgt aaattttttg aaataagagg ccatcttctt
    12061 ccgtaatatg agggtagtct aaatctttta gtgattggct gatccgatgt gctcgcatat
    12121 attctagtgt ccaatctcct gccattactt ttgggaagtt ttcatcacta tgatggcgga
    12181 accggatata tagtggatgc atatctgcat ctgaaacaag cggaacgact cttttgaccg
    12241 cgtttcgaaa tggctgctgc tgatcataca tcgtatcatc aacatcaaac acaactgatt
    12301 taatcaaaat tttacacgcc tttcggtttt cattgatttt ttcacaaatc cttgactatt
    12361 gtaacagaaa aaattgtcta aaaaagctga taaataaagg tttttcttac tatatttcta
    12421 tttccttgac agaataaggt ttttatattt cagttttttt cactaaaaat cgtgaaacac
    12481 gtcaataagt ttgatgaaca aattaatcat tatggtagaa gtcgattgat ttattttaat
    12541 gcaatctctg tggaattttc gaaagattta tttttttaat acagtctcaa tataactatt
    12601 caaacataaa ataacgttat ttgatttatt tgtttatagt aaattttatt tagtaatc
//
LOCUS       DAZKMK010000083        12493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000083 DAZKMK010000000
VERSION     DAZKMK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.085"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8V1_002215"
     CDS             <1..375
                     /locus_tag="ACG8V1_002215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330271.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG8V1_002216"
     CDS             complement(324..893)
                     /locus_tag="ACG8V1_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0330272.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG8V1_002217"
     CDS             complement(967..2256)
                     /locus_tag="ACG8V1_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330273.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG8V1_002218"
     CDS             2514..3458
                     /locus_tag="ACG8V1_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0330274.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG8V1_002219"
     CDS             3472..4545
                     /locus_tag="ACG8V1_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0330275.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG8V1_002220"
     CDS             4535..6706
                     /locus_tag="ACG8V1_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0330276.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8V1_002221"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8V1_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0330277.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG8V1_002222"
     CDS             complement(9009..11195)
                     /locus_tag="ACG8V1_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0330278.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8V1_002223"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8V1_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0330279.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG8V1_002224"
     CDS             complement(11417..11857)
                     /locus_tag="ACG8V1_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0330280.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG8V1_002225"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG8V1_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2631 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aac
//
LOCUS       DAZKMK010000084        12474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000084 DAZKMK010000000
VERSION     DAZKMK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.086"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..764)
                     /locus_tag="ACG8V1_002226"
     CDS             complement(111..764)
                     /locus_tag="ACG8V1_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0330281.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1052..1572
                     /locus_tag="ACG8V1_002227"
                     /pseudo
     CDS             1052..1572
                     /locus_tag="ACG8V1_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1570..2289)
                     /locus_tag="ACG8V1_002228"
                     /pseudo
     CDS             complement(1570..2289)
                     /locus_tag="ACG8V1_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2240..2449)
                     /locus_tag="ACG8V1_002229"
     CDS             complement(2240..2449)
                     /locus_tag="ACG8V1_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330282.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(2431..2499)
                     /locus_tag="ACG8V1_002230"
                     /pseudo
     CDS             complement(2431..2499)
                     /locus_tag="ACG8V1_002230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            2726..3999
                     /locus_tag="ACG8V1_002231"
                     /pseudo
     CDS             2726..3999
                     /locus_tag="ACG8V1_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4106..5137)
                     /locus_tag="ACG8V1_002232"
     CDS             complement(4106..5137)
                     /locus_tag="ACG8V1_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330283.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5144..5974)
                     /locus_tag="ACG8V1_002233"
     CDS             complement(5144..5974)
                     /locus_tag="ACG8V1_002233"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0330284.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5971..6786)
                     /locus_tag="ACG8V1_002234"
     CDS             complement(5971..6786)
                     /locus_tag="ACG8V1_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0330285.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6783..7607)
                     /locus_tag="ACG8V1_002235"
     CDS             complement(6783..7607)
                     /locus_tag="ACG8V1_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0330286.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7597..8697)
                     /locus_tag="ACG8V1_002236"
     CDS             complement(7597..8697)
                     /locus_tag="ACG8V1_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0330287.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8875..9660
                     /locus_tag="ACG8V1_002237"
     CDS             8875..9660
                     /locus_tag="ACG8V1_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0330288.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9693..10076
                     /locus_tag="ACG8V1_002238"
     CDS             9693..10076
                     /locus_tag="ACG8V1_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0330289.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10152..10283
                     /locus_tag="ACG8V1_002239"
     CDS             10152..10283
                     /locus_tag="ACG8V1_002239"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0330290.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10252..10488
                     /locus_tag="ACG8V1_002240"
     CDS             10252..10488
                     /locus_tag="ACG8V1_002240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0330291.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            10500..10586
                     /locus_tag="ACG8V1_002241"
                     /pseudo
     CDS             10500..10586
                     /locus_tag="ACG8V1_002241"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(10566..11537)
                     /locus_tag="ACG8V1_002242"
     CDS             complement(10566..11537)
                     /locus_tag="ACG8V1_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0330292.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12189..>12474
                     /locus_tag="ACG8V1_002243"
     CDS             12189..>12474
                     /locus_tag="ACG8V1_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0330293.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3746 a         2810 c         2289 g         3629 t
ORIGIN      
        1 caaatatgtt ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc
       61 aagaacagaa aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc
      121 tccaatttat atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta
      181 agttctttta tatatacatc tattactcga tcataaggag tttcttcgct atctttccat
      241 acataatcaa taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt
      301 aatacttcta gttcttttgc atttacatct atttcttcac catttatttt agctgtatag
      361 ctattaaaat taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc
      421 ttaattaaag aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat
      481 ccgtctacta aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag
      541 attagaattg gtaaattgct tttctttcta atttctttca acacttctaa accatttata
      601 aaaggtatct gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct
      661 tctcttccat ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt
      721 ccctctctta tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa
      781 gcctatacac tagatattat acagcatatt atggctctat aatatctagt gttggtgacg
      841 ccttctgttg ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc
      901 tttagaaaaa aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac
      961 taacactttt tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac
     1021 caaaacatca ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg
     1081 attaacagat aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg
     1141 cgttcaagcg cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg
     1201 tggatcgatt cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt
     1261 caaagaaaag ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg
     1321 gcagaccttt tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact
     1381 gaagcaggcg attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag
     1441 aagtaccgtc ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag
     1501 ccgctcaaac ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc
     1561 gatgaaccgt tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc
     1621 agtcgccaca tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg
     1681 ttcaggatct tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac
     1741 gtaggcaacc agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta
     1801 atcgattgct cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca
     1861 tgaactatcg taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat
     1921 ttgttctaac aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt
     1981 atgcccgcta aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg
     2041 tgctgcttgg gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg
     2101 tttcaagcac aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt
     2161 cacaaaggaa tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc
     2221 ccgcagttca gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat
     2281 gttcagcatc cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg
     2341 ctgccgtccc aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca
     2401 gtaaataggc ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat
     2461 catcgtccca tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa
     2521 acaagcaaaa gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa
     2581 agtcaaaggt gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat
     2641 cagtcctttt gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca
     2701 caacacaatc aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg
     2761 aatcaaagat ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa
     2821 cagtatcgtt gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca
     2881 ggtgaatcaa catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa
     2941 taagtgtgcg aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg
     3001 ctgtcacacc tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa
     3061 acaagtacat tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc
     3121 taaacattgc ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat
     3181 gacaaacgtg aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt
     3241 gaaaaatcaa gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact
     3301 ggatattctt ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg
     3361 acgtgcacgt ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct
     3421 gataaaagag tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca
     3481 cctcattcgc agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga
     3541 tccaatacaa gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg
     3601 acaggacacg cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt
     3661 aaccgaaagt gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta
     3721 tgcttactat caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca
     3781 gcttgtaaaa acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt
     3841 tataaactat gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat
     3901 agaaaatctg catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga
     3961 accagaattt tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc
     4021 agaaaatcaa ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga
     4081 gccagaaatg tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt
     4141 ccacagggat tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt
     4201 cccgatcgtt caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc
     4261 gttgaatgtt gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag
     4321 gaaccggttc aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag
     4381 cggaaaactc gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga
     4441 taaattccgc aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct
     4501 gagccacccg cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt
     4561 ggtcatgagt cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc
     4621 gctgcagctg ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga
     4681 ctttcggttc agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa
     4741 gaaactggac atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa
     4801 ccttttcctg aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt
     4861 caaaaacagt catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat
     4921 ccttcgtagg cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt
     4981 gttccaatcc cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta
     5041 aagtaaccag ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat
     5101 caaaaaacag attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg
     5161 gcttcttttt cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc
     5221 gataagtcag cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca
     5281 tcacagcgct agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg
     5341 tctgaaatcg accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa
     5401 aaatcatcag agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa
     5461 taatcgtaga catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa
     5521 ttaagcgcat ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg
     5581 cacctaccaa cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg
     5641 ttaccaaaat cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat
     5701 tcaaacgagg gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg
     5761 tcaccaagac cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt
     5821 ccgtcagcag ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc
     5881 atttagtgga gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa
     5941 agcagaacaa aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg
     6001 aataatcaac attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa
     6061 attaggcttg gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt
     6121 tacattactg ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa
     6181 gatgacaaag gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc
     6241 atacgctgat gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt
     6301 catgctggga tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt
     6361 caatccccac caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac
     6421 acctacattc cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc
     6481 tgccaaattg gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca
     6541 taaacataaa ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac
     6601 gctgttaaca aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa
     6661 tgtccactgg ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag
     6721 cagtaaaaat gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc
     6781 tttcatcccc tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat
     6841 aaccagcaaa acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg
     6901 cgtttatttg aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc
     6961 agtccgtgcg catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc
     7021 cattcaggaa ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca
     7081 tgagaatcgg cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa
     7141 aagtctggct gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg
     7201 taagtgtcct cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg
     7261 cgatcaacca aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg
     7321 gcggctcctg tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat
     7381 cgctgagcat ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt
     7441 acttcgtata acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct
     7501 tgctggtgct caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga
     7561 cagccgtcta gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc
     7621 cgcctgccaa agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac
     7681 tggcaccgcc gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag
     7741 ctctaccttg cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga
     7801 taaaatatat tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa
     7861 ggttgtatac ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga
     7921 gtcaatttgg tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc
     7981 acctttttca agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat
     8041 ttttttgtag taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc
     8101 cgcagccaat acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg
     8161 atagtcaccc tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc
     8221 ggtgataaag gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc
     8281 ccaatccgga atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc
     8341 agccaacgga ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc
     8401 tgattcaaac ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc
     8461 gtattctttt tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc
     8521 caagctggca atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc
     8581 tgccgcagcc gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac
     8641 tgttgctgca caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca
     8701 ttcttctccc ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc
     8761 ttgaaaaggt ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc
     8821 ccgttaacct tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag
     8881 tattatgatc agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac
     8941 tatttagcct atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca
     9001 tctaccggaa aagatgacat tccggattat ccagcctata taaacaaact ggactcttta
     9061 aaagaaacct attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa
     9121 gaagagcgga tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt
     9181 catcaaaatg gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa
     9241 actaccaagg aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat
     9301 attttgactc actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg
     9361 gagcattttg aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg
     9421 atggagttaa atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc
     9481 gttcctttgt atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt
     9541 gccgaagatt atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta
     9601 gaaaagttgc tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga
     9661 ttcctatata atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag
     9721 aggcacccat ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt
     9781 gccgatcctt gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca
     9841 gcttgcagcg ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat
     9901 atttgcatgc gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt
     9961 tacgaggaca gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg
    10021 gaatggctcg ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc
    10081 aggatcaagg cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg
    10141 aagaagtttg tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc
    10201 aagctcccct ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca
    10261 cacctttcgg ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct
    10321 ggtgatgaag ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt
    10381 tatcatttta atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag
    10441 ttttttattg tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact
    10501 tggagcttgg aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc
    10561 aagggttaga aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga
    10621 ttgagccagt gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact
    10681 gacttcagca aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc
    10741 cataagataa agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc
    10801 aaatgaggaa ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc
    10861 gctggcgtgt ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag
    10921 cagtggtacg gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca
    10981 atcgtcagca gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc
    11041 acttcgcagt taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta
    11101 tctaagccgg tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat
    11161 gtatccacat agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc
    11221 agtacggtat tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc
    11281 tgaaccagtg cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca
    11341 gcatccttct caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt
    11401 tcaatctgct tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc
    11461 tttaaataaa caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt
    11521 aatactgttc cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa
    11581 ggaaaaactt accatttaat cataaaatta gctcatataa atatactagg atgtaaaata
    11641 gactggtcaa aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt
    11701 ttaatattct aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac
    11761 tatatcacta gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta
    11821 aatggtcatt gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg
    11881 ctgagagtaa aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt
    11941 atttcagatt accttaaatg attccattta ctttttatct cattttttcc tactagttag
    12001 tatcaaatac tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag
    12061 ctaaaacctt gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt
    12121 tatggatgat gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag
    12181 tgatagctat gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga
    12241 ttgatgatct agttcctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg
    12301 aatttattta tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc
    12361 cagtgattct gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac
    12421 aaaccattaa ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tact
//
LOCUS       DAZKMK010000085        12421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000085 DAZKMK010000000
VERSION     DAZKMK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.087"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8V1_002244"
     CDS             <1..435
                     /locus_tag="ACG8V1_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0330294.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8V1_002245"
     tRNA            complement(759..842)
                     /locus_tag="ACG8V1_002245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8V1_002246"
     CDS             1370..1870
                     /locus_tag="ACG8V1_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0330295.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG8V1_002247"
     CDS             complement(2060..2404)
                     /locus_tag="ACG8V1_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0330296.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG8V1_002248"
     CDS             complement(2558..3478)
                     /locus_tag="ACG8V1_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0330297.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG8V1_002249"
     CDS             3665..4156
                     /locus_tag="ACG8V1_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0330298.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG8V1_002250"
     CDS             complement(4180..6555)
                     /locus_tag="ACG8V1_002250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0330299.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACG8V1_002251"
     CDS             6881..7507
                     /locus_tag="ACG8V1_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0330300.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACG8V1_002252"
     CDS             7530..8234
                     /locus_tag="ACG8V1_002252"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0330301.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACG8V1_002253"
     CDS             8426..9259
                     /locus_tag="ACG8V1_002253"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0330302.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..10940
                     /locus_tag="ACG8V1_002254"
                     /pseudo
     CDS             9456..10940
                     /locus_tag="ACG8V1_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            11082..12356
                     /locus_tag="ACG8V1_002255"
     CDS             11082..12356
                     /locus_tag="ACG8V1_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0330303.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         3838 a         2401 c         2243 g         3939 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa aggttctata atattttgtg ttggtggaaa atcgaaagcg
    10981 attttctact aacacttttt tcttcgtttc aactcaaaaa gagactcact tttggtaaaa
    11041 tttagtcgac caaaacgtca ccctaccaaa ggagtctctc aatggataat catactagaa
    11101 aattactcgg attaacagat aaacatttat tttttgatga agaatggctg attgaaaaag
    11161 aatataaagg cgttcaagcg cagtttatta aagggagatt ggatgactca ccttcccatt
    11221 gtgaacactg tggatcgatt cgtattatcc gcaacggttc ctatacgaca cgcactcaga
    11281 tgttgaaagt caaagaaaag ttgactattt tagaattaaa acgtacccgt tttctctgcc
    11341 atgattgtgg acggaccttt tctgcgaaaa cggacttagt tgatgaacat catcaactaa
    11401 caaaggaact gaagcaggcg attctcatgg aactgtacga aaatcaatca cgtaaactaa
    11461 tcgctaagaa gtacttcgtc tcggatggga cggtgacacg gatcttgcgt gaagcaacga
    11521 agcattacca gccacgtatg aacttccttc cgacggtgtt gtgtatggat gagttcaaat
    11581 caatgaagtc tgtttctggt tccatgagtt ttatttgtgt tgatgggaca accaatcagc
    11641 tatttacgat ccttgaagat cgcagacttt acaaactaac tcaatacttc ctgcgctttc
    11701 ctagaaaggc ccgtttaaag gtcaagtacc tagtgatgga catgaatgcc agctattgtc
    11761 agttgcttaa aacagtcttt ccaaatgcgg agatcgtaac tgaccgcttt catattgtcc
    11821 aacacatcaa tcgcagcttc aatcaattac gtgtccagat catgaatcgt tttcgaacct
    11881 cacactctga aaatcagaag aagtaccgtc ggctcaagcg atattggaaa ctcttactca
    11941 aagattccac gacattggag ccactcaaac ggcattatca tcgactgttt aaacgaccaa
    12001 ttagtcaaac agaaatcgtt gatgagctgt taagctacaa cgaagaattg cgaactgcgt
    12061 atcatttttg tcaattatta cgctattatt tcgttaaacg agatacggaa ggctttcaag
    12121 aaacccttaa aacaatttca tctacgttac ctcagtcgtt taaaaagaaa ttcacgatct
    12181 tccatcgcta ccaatcgggt atttcaaatg cctttaaggt gccccactca aacggcgtca
    12241 ctgaggggct gaataacaag atcaagctga ttaaacgaat tgcgtttggt tatcgcaact
    12301 tttataactt tcgggcacgg atctatttac aacaaggtct gatctttgaa aactaaaaaa
    12361 atatgcggcg cacagaacgc gccgcatatc ggactaagtt ttattcaaat ttgtcttcgc
    12421 c
//
LOCUS       DAZKMK010000086        12335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000086 DAZKMK010000000
VERSION     DAZKMK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.088"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8V1_002256"
     CDS             <1..154
                     /locus_tag="ACG8V1_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0330304.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG8V1_002257"
     CDS             complement(208..486)
                     /locus_tag="ACG8V1_002257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330305.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG8V1_002258"
     CDS             complement(601..1218)
                     /locus_tag="ACG8V1_002258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330306.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG8V1_002259"
     CDS             complement(1613..2437)
                     /locus_tag="ACG8V1_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0330307.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG8V1_002260"
     CDS             2780..3196
                     /locus_tag="ACG8V1_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0330308.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG8V1_002261"
     CDS             3193..3663
                     /locus_tag="ACG8V1_002261"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0330309.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG8V1_002262"
     CDS             3677..4450
                     /locus_tag="ACG8V1_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0330310.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG8V1_002263"
     CDS             4464..5276
                     /locus_tag="ACG8V1_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0330311.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG8V1_002264"
     CDS             5330..6295
                     /locus_tag="ACG8V1_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0330312.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG8V1_002265"
     CDS             6431..9154
                     /locus_tag="ACG8V1_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0330313.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG8V1_002266"
     CDS             9167..9799
                     /locus_tag="ACG8V1_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0330314.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG8V1_002267"
     CDS             10007..10231
                     /locus_tag="ACG8V1_002267"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0330315.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG8V1_002268"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG8V1_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0330316.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG8V1_002269"
     CDS             10556..10825
                     /locus_tag="ACG8V1_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0330317.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACG8V1_002270"
     CDS             complement(11004..12305)
                     /locus_tag="ACG8V1_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0330318.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4029 a         1998 c         2422 g         3886 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtg
//
LOCUS       DAZKMK010000087        12201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000087 DAZKMK010000000
VERSION     DAZKMK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.089"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..650)
                     /gene="frr"
                     /locus_tag="ACG8V1_002271"
     CDS             complement(93..650)
                     /gene="frr"
                     /locus_tag="ACG8V1_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0330319.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(653..1375)
                     /gene="pyrH"
                     /locus_tag="ACG8V1_002272"
     CDS             complement(653..1375)
                     /gene="pyrH"
                     /locus_tag="ACG8V1_002272"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0330320.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1511..2392)
                     /gene="tsf"
                     /locus_tag="ACG8V1_002273"
     CDS             complement(1511..2392)
                     /gene="tsf"
                     /locus_tag="ACG8V1_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0330321.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="ACG8V1_002274"
     CDS             complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="ACG8V1_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0330322.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3632..4111
                     /locus_tag="ACG8V1_002275"
     CDS             3632..4111
                     /locus_tag="ACG8V1_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0330323.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4328..5419
                     /gene="phnW"
                     /locus_tag="ACG8V1_002276"
     CDS             4328..5419
                     /gene="phnW"
                     /locus_tag="ACG8V1_002276"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0330324.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5412..5540
                     /locus_tag="ACG8V1_002277"
     CDS             5412..5540
                     /locus_tag="ACG8V1_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0330325.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5544..6089
                     /locus_tag="ACG8V1_002278"
     CDS             5544..6089
                     /locus_tag="ACG8V1_002278"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0330326.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6543..8234)
                     /gene="argS"
                     /locus_tag="ACG8V1_002279"
     CDS             complement(6543..8234)
                     /gene="argS"
                     /locus_tag="ACG8V1_002279"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0330327.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG8V1_002280"
     CDS             complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG8V1_002280"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0330328.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG8V1_002281"
     CDS             complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG8V1_002281"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0330329.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG8V1_002282"
     CDS             complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG8V1_002282"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0330330.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3342 a         2529 c         2203 g         4127 t
ORIGIN      
        1 ataatttttt ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg
       61 gaatgatccg cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta
      121 tcagcagcga tcttatctac ttctttgatg ctgttatctg tcaatgtttg tacgtctttt
      181 tctaatccgc gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca
      241 tcgatcgcat cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc
      301 acatctttag ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg
      361 acatttccat cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca
      421 tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg
      481 attgatgcca tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc
      541 aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt
      601 gctgcttttt tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc
      661 cccttacagt tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat
      721 ttaaattgaa gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat
      781 ccatgacttg taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg
      841 catttgcatc tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca
      901 ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt
      961 acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg
     1021 ctttacggcg aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg
     1081 tcggtacacc cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta
     1141 acatacccat gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc
     1201 gccagatatt tccgccgccc acaacgattg ccatttcaac gcccagctcg tgaacttctt
     1261 tgatttcttg gatgatttct ttgattactg gtggtttgat tccaaatcct gcgtccccag
     1321 ctaaagcttc tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca
     1381 ttcctccaat attttctatt gccattttac catacttaga tgatttcgac actctaagac
     1441 tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta
     1501 ttcaacagaa ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt
     1561 tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg
     1621 caacgtattt ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga
     1681 tttctgcttt gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt
     1741 tgccttcgtt caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg
     1801 gaatttgtga ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc
     1861 ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca
     1921 tatgcaagta tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga
     1981 agctgatttt ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt
     2041 tatctgtttt gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt
     2101 ctgcaatttc ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg
     2161 agtttacttc aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag
     2221 ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa
     2281 ctgctttttc catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta
     2341 cgccagtcat ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact
     2401 cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc
     2461 cagcctttga aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac
     2521 aacatctacg atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac
     2581 tacttgatct tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa
     2641 tttaacggca cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc
     2701 gcagtttgta tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc
     2761 aatacgttct ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc
     2821 gataccgcct aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt
     2881 tttaggtaga acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct
     2941 gatacgtttt tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac
     3001 gaagtattgt cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt
     3061 acctacgaat aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc
     3121 atctactaat ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa
     3181 gatatatttc ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc
     3241 ttctagtaat tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt
     3301 tttcctcttc ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca
     3361 ataagaatgt ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc
     3421 cgtatcgcaa gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa
     3481 aaagaattca agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa
     3541 ataattttag gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata
     3601 catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat
     3661 taaacaagtc attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg
     3721 gcattatggc gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat
     3781 cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac
     3841 tcctgagact aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt
     3901 gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt
     3961 atttgcatct gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt
     4021 cgatacgatc atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct
     4081 tcaccatcca actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc
     4141 gtgttttttt attttcaata ttttccttat tgaataaacc tttacactct ttacaaaaat
     4201 acatcatttt ttattcaaat ttttcaacac cttgattgaa tcacaataat catcatttat
     4261 attggatgtt aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg
     4321 aaatcatatg tataaattat taacttctgg accattgacg acttctgaaa cagtgaaaaa
     4381 tgctatgctg atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat
     4441 aagaaaaaaa ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat
     4501 gcaaggtagc agctccttcg gggtagaatc ggtattaagt tctgtgatca ggccagaaga
     4561 tactgtactg atttgttcaa acggcgcata tagacaacga atgatcaaca tgtgcgaatg
     4621 tctgaaactg aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt
     4681 gattacacag ctgattgaag agaatccgtc tatctccgca ctagcaatgg tccatagcga
     4741 gactacttcc ggtattttga ataatttgga agagattgct gctgtcacac aagaaagaga
     4801 tcttttgttt atagtggacg cgatgtcaag ctttggtggg ataccgattt gtgtagccga
     4861 actagggatt gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt
     4921 ttcttttgta cttgctaata aagcggaact ggaaaaacga aaaaatgtta gccgaagttt
     4981 gtcacttgat ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt
     5041 cacttcccct acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga
     5101 aggaggaatc gaaactcgat attcacgtta tctcgccaat aatcatgtgc tacgacaaaa
     5161 aatgacagag atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc
     5221 ttttctttac cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca
     5281 aaatggattt gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa
     5341 tattggcgag atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa
     5401 ggagaacgtt aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg
     5461 gctgtatggc tcctgtccac gcttttagaa atgcttttct agaaaaagga acccaactga
     5521 ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc
     5581 tttccctccc ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa
     5641 aagaggtgga ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc
     5701 attctacctt gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca
     5761 atattggttc cacgaatgga tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa
     5821 aaaaagaaag gtattcacca gacttcatgg caacttttga agacgtcgaa gggtatggtc
     5881 gaccgtttcc ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg
     5941 aagtaatcaa agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt
     6001 tgacagtagg tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa
     6061 aattagagat ggggaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc
     6121 cggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga
     6181 caagaatcca gaatgacttt tatacaaaat aagaggttgt gaaaaagctg tcctgtatca
     6241 agtaataaaa acagccaagc aaaaatagct cctcatattt ttcgtttggt taggcttatt
     6301 accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg
     6361 acttttgtca cagtcccctt atttgcaaac aaacagtgtt taagagacaa cctctccact
     6421 aaacggtgtt cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag
     6481 ctattttctc ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt
     6541 tcttacatct tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca
     6601 gtcactgcat aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc
     6661 gtatgagcat agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact
     6721 gacggttcgt atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta
     6781 accacttccc agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat
     6841 cctgcttttt ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt
     6901 tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt
     6961 ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt
     7021 tccaaatcag ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat
     7081 acttcttcta ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg
     7141 atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt caaagcccat ttccttcaag
     7201 acagctttca actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt
     7261 ttgaagtcgt aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt
     7321 gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg
     7381 atctctgcac ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca
     7441 tccatcttgt cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga
     7501 tcatagattt tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata
     7561 gcctctttat cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct
     7621 ggttctgttt cagctacttc atggaattgg acgtataaac gaagcaattc attgattggt
     7681 tctgctttaa cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac
     7741 tgtgttcccc agtcacctaa atggttgatg cggattggct gataaccgat tttttccata
     7801 atgaaaccga tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg
     7861 gcaatattag gagaagacat gtcgattggt actgttcctt gattcccgat gttgctatcc
     7921 ccataatgtt ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta
     7981 ttcatgaaaa agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg
     8041 attttttctg ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat
     8101 gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgc
     8161 tcgacttgtt ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga
     8221 tctttattat tcatctttat tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat
     8281 ctctagcgac ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact
     8341 ccaattgtcc taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa
     8401 acagaattct ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa
     8461 ttcgctagcc aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac
     8521 tttcttgaat tatatcagaa aaaaaaacga aaggctagtc gtttcataaa aaaaaaatcc
     8581 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8641 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8701 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8761 tagctgcttc tactttcgga agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt
     8821 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8881 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8941 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     9001 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9061 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9121 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9181 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9241 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9301 ccttcagtac ttcgcccatg gcgttttgta accagtaacc gatagatcct tctgtcattg
     9361 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9421 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9481 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9541 ttgcatccgt agacaagatg gcattgcccc ctaaagcgac aactactttt cgtttggcca
     9601 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9661 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9721 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9781 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9841 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9901 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9961 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
    10021 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10081 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10141 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10201 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10261 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10321 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10381 gttcctctac cattttttga ctgaatccac ggaattcgat tccgtcaaac atacgtccta
    10441 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10501 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10561 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10621 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10681 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10741 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10801 agtaagactg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10861 tgcagcgtgg accaccacga cctcgtgaca attcacttga attgatttct aacactttga
    10921 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10981 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    11041 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11101 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11161 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11221 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgttcgga attttgattg
    11281 ccaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11341 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11401 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11461 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11521 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11581 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11641 catatagact gctagaacgt acttcgatat cttttgtacg aaccccagcc atgattttat
    11701 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11761 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11821 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11881 atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11941 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccgtttca
    12001 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12061 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12121 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    12181 acgtttttct atttttaagc g
//
LOCUS       DAZKMK010000088        11439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000088 DAZKMK010000000
VERSION     DAZKMK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.090"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..619)
                     /locus_tag="ACG8V1_002283"
     CDS             complement(134..619)
                     /locus_tag="ACG8V1_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0330331.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    709..1054
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1099..1740
                     /locus_tag="ACG8V1_002284"
     CDS             1099..1740
                     /locus_tag="ACG8V1_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0330332.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1759..2394
                     /locus_tag="ACG8V1_002285"
     CDS             1759..2394
                     /locus_tag="ACG8V1_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330333.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2412..3227
                     /locus_tag="ACG8V1_002286"
     CDS             2412..3227
                     /locus_tag="ACG8V1_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0330334.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3546..4982
                     /locus_tag="ACG8V1_002287"
     CDS             3546..4982
                     /locus_tag="ACG8V1_002287"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0330335.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5171..7618
                     /locus_tag="ACG8V1_002288"
     CDS             5171..7618
                     /locus_tag="ACG8V1_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0330336.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7848..8915
                     /gene="rlmN"
                     /locus_tag="ACG8V1_002289"
     CDS             7848..8915
                     /gene="rlmN"
                     /locus_tag="ACG8V1_002289"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0330337.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8905..9111
                     /locus_tag="ACG8V1_002290"
     CDS             8905..9111
                     /locus_tag="ACG8V1_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330338.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9108..10211)
                     /locus_tag="ACG8V1_002291"
     CDS             complement(9108..10211)
                     /locus_tag="ACG8V1_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0330339.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10764..11312
                     /gene="aac(6')"
                     /locus_tag="ACG8V1_002292"
     CDS             10764..11312
                     /gene="aac(6')"
                     /locus_tag="ACG8V1_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0330340.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3795 a         1939 c         2470 g         3235 t
ORIGIN      
        1 gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa
       61 gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa
      121 aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct
      181 tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta
      241 cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt
      301 gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt
      361 cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg
      421 cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata
      481 atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg
      541 caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt
      601 gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa
      661 gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg
      721 aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca
      781 gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg
      841 gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg
      901 agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac
      961 tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa
     1021 acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt
     1081 ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga
     1141 gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc attagggatt
     1201 gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat
     1261 gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg
     1321 ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac
     1381 tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa
     1441 tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc
     1501 ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa
     1561 gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg
     1621 atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg
     1681 actggtattc taacaatcat aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa
     1741 ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa
     1801 aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt
     1861 ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac
     1921 agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa
     1981 caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa
     2041 aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa
     2101 gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag
     2161 ctttctggcg gacaaaaaca gcgggtagct attgccagag cattggctat gaatccacgg
     2221 gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag
     2281 cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta
     2341 gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg
     2401 agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt
     2461 ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga
     2521 aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg
     2581 ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta
     2641 cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga
     2701 tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta
     2761 caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa
     2821 actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg
     2881 tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt
     2941 atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga
     3001 tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca
     3061 taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta
     3121 cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa
     3181 aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact
     3241 gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc
     3301 ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca
     3361 gtttttcggt attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt
     3421 tcttactact cgaaactaag cgactttttc acaacctcct ttacataatt ttattttgtt
     3481 gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga
     3541 gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt
     3601 ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg
     3661 atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag
     3721 accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt
     3781 accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag
     3841 ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta
     3901 tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg
     3961 aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc
     4021 gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt
     4081 atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc
     4141 atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg
     4201 gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa
     4261 atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata
     4321 tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa
     4381 cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca
     4441 attactatca aacggcgacg aatgcattca atccattaaa cggagttggt gctggaaaaa
     4501 tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa
     4561 aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat
     4621 taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa
     4681 atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa
     4741 tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag
     4801 tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa
     4861 agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat
     4921 ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt
     4981 agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa
     5041 atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt
     5101 ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga
     5161 gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac
     5221 gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat
     5281 tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg
     5341 ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg
     5401 attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg
     5461 cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact
     5521 ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca gtgatataaa
     5581 acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt
     5641 tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg
     5701 aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat
     5761 tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc
     5821 ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc
     5881 aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca
     5941 aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag
     6001 ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt
     6061 accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga
     6121 tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt
     6181 accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa
     6241 aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa
     6301 ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg
     6361 ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa
     6421 agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa
     6481 tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga
     6541 taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct
     6601 ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat
     6661 taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat
     6721 gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc
     6781 ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga
     6841 agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt
     6901 tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt
     6961 gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat
     7021 tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg
     7081 tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt
     7141 tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct
     7201 cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca
     7261 aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca
     7321 agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc
     7381 agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac
     7441 ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt
     7501 agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa
     7561 agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa
     7621 cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc
     7681 aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag
     7741 tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa
     7801 tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt
     7861 ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa
     7921 aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag
     7981 aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc
     8041 ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac
     8101 tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt
     8161 gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca
     8221 aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt
     8281 attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg
     8341 agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag
     8401 gattggcaat tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca
     8461 aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata
     8521 atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg
     8581 aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt
     8641 tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata
     8701 agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat
     8761 attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg
     8821 taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc
     8881 gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt
     8941 accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat
     9001 tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat
     9061 gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc
     9121 ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt
     9181 ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct
     9241 ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact
     9301 ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc
     9361 atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt
     9421 ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg
     9481 ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt
     9541 gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga
     9601 cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg
     9661 ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat
     9721 aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt
     9781 tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca
     9841 gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt
     9901 tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct
     9961 gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc
    10021 ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt
    10081 tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg
    10141 acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca
    10201 ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg
    10261 atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga
    10321 ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa
    10381 aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt
    10441 tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga aaacaggttt
    10501 tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac
    10561 aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg
    10621 tttctgtata atttttttat tttataagta agataaataa tgaaagaata gagatactga
    10681 ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata
    10741 agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat
    10801 tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct
    10861 cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc
    10921 aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat
    10981 tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca
    11041 attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg
    11101 atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag
    11161 tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata
    11221 aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa
    11281 aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca
    11341 gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa
    11401 cttatttccg aagaagctac ttttggaaca ccgtgtacc
//
LOCUS       DAZKMK010000089        11199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000089 DAZKMK010000000
VERSION     DAZKMK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.091"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..898)
                     /gene="ftsX"
                     /locus_tag="ACG8V1_002293"
     CDS             complement(14..898)
                     /gene="ftsX"
                     /locus_tag="ACG8V1_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0330341.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(891..1577)
                     /gene="ftsE"
                     /locus_tag="ACG8V1_002294"
     CDS             complement(891..1577)
                     /gene="ftsE"
                     /locus_tag="ACG8V1_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0330342.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1682..2783)
                     /gene="prfB"
                     /locus_tag="ACG8V1_002295"
     CDS             complement(join(1682..2710,2712..2783))
                     /gene="prfB"
                     /locus_tag="ACG8V1_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0330343.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2909..5443)
                     /gene="secA"
                     /locus_tag="ACG8V1_002296"
     CDS             complement(2909..5443)
                     /gene="secA"
                     /locus_tag="ACG8V1_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0330344.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5665..6216)
                     /gene="raiA"
                     /locus_tag="ACG8V1_002297"
     CDS             complement(5665..6216)
                     /gene="raiA"
                     /locus_tag="ACG8V1_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0330345.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6344..7063)
                     /locus_tag="ACG8V1_002298"
     CDS             complement(6344..7063)
                     /locus_tag="ACG8V1_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0330346.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7060..8373)
                     /locus_tag="ACG8V1_002299"
     CDS             complement(7060..8373)
                     /locus_tag="ACG8V1_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0330347.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8454..8774)
                     /locus_tag="ACG8V1_002300"
     CDS             complement(8454..8774)
                     /locus_tag="ACG8V1_002300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0330348.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8882..9169)
                     /locus_tag="ACG8V1_002301"
     CDS             complement(8882..9169)
                     /locus_tag="ACG8V1_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330349.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9296..9940
                     /locus_tag="ACG8V1_002302"
     CDS             9296..9940
                     /locus_tag="ACG8V1_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0330350.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9999..10706)
                     /locus_tag="ACG8V1_002303"
     CDS             complement(9999..10706)
                     /locus_tag="ACG8V1_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0330351.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10926..11096)
                     /locus_tag="ACG8V1_002304"
     CDS             complement(10926..11096)
                     /locus_tag="ACG8V1_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330352.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3759 t
ORIGIN      
        1 ttatattgtg gtattagaat ttcaaaaaac gtctcataga aatgatcgag ccaagtgaac
       61 caatgatcac accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggac
      121 gaatcaatga ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat
      181 aaccaaatgt gatgatcaaa atcggaagaa tcgcaccgat cagaccgatc cagccacctt
      241 caaggaaaaa cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa
      301 tttcacgctg tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta
      361 cgaacagcag caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga
      421 aaatccgatc tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg
      481 cttcttctgc tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat
      541 ataacgggtt attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt
      601 ttttcaattc ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca
      661 attctttttc tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata
      721 catcaacgtt tccttctata tcctgagcta atttagttgc attcatgata actgccatga
      781 atacaccgac taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc
      841 cgttacgttt caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg
      901 tatccatatt ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact
      961 cggtgccgaa tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct
     1021 tgggcattga tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt
     1081 cctgtcggtt catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag
     1141 acacgttgct gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa
     1201 ccaacaaggt ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata
     1261 acttgcatag cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct
     1321 tggaacacca cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt
     1381 agatcatgac ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat
     1441 ttgatgaatg tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt
     1501 tcgatgacaa tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca
     1561 tccttcattt caatcatggc gttctctcca tatctttttc agtctttagc tattatagca
     1621 tggcaatgtt tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgagtt
     1681 cttattgtag atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa
     1741 ctgcttggac atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat
     1801 ggaaaacata agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg
     1861 cagctgcttc ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc
     1921 ccatcgcttg ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc
     1981 ctgtaggcaa gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg
     2041 caccacttgc tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg
     2101 tttcatcaag ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg
     2161 agtcaaaagg cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa
     2221 aagcgttatg tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt
     2281 gataatctag tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta
     2341 aaagcatgct gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga
     2401 tcgcattgtt tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt
     2461 tttcatctaa tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt
     2521 cttgcagcat ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt
     2581 ggtcataggt ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt
     2641 catcccaaaa accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct
     2701 gatctaagtc aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga
     2761 tttcgaattt cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat
     2821 ctagtctaac agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa
     2881 tttgtctcac caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat
     2941 attttttccc gcttccacaa ggacaaggat catttcttcc gaccttgttc acatggattg
     3001 gctgtttttt cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag
     3061 ctgttccttg agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga
     3121 ttcgagtgac ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg
     3181 tttgatattc gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta
     3241 attgatccat tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga
     3301 ctttttcaaa ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata
     3361 cttcttgtgc tcgttcgttt agatactctt tgatctcctg agatgttttt ccttcgatat
     3421 ctgcaagagt aatactatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt
     3481 ccaaattcca attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc
     3541 gtttgaccat gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc
     3601 gctgtttata gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt
     3661 tacgtgtgtc atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca
     3721 gcatcttgct ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt
     3781 tgattcgttc tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaattgtg
     3841 acataccagg gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag
     3901 attcatgtcg ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta
     3961 acttgatatc tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac
     4021 cagcattcat gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag
     4081 gaatacgttc ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc
     4141 cgactaacac aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga
     4201 acttgctttg taatgttggg taaagtaaat ctggacgatc atcacggata accggacgat
     4261 ttgtcggaat ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt
     4321 tagctgtacc agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga
     4381 tcgtcgccat ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt
     4441 ggtgaagtcc atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa
     4501 tcagtacttt tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc
     4561 tcaatgcttg atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca
     4621 aaccgaatgt ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt
     4681 gtacatcgat tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg
     4741 tatataaagc tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat
     4801 caatcaagat tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt
     4861 gatggcggta aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac
     4921 tataagtgat atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat
     4981 tcaaccccac tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg
     5041 ttgctaaata ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat
     5101 atactggcat cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc
     5161 catcgtgaag cacgattcca cccatcaatt gtacgtggta tggatataac cccaatacac
     5221 gttttgctgc ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc
     5281 ctttttggta tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg
     5341 ctgccatttg gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt
     5401 ctttcttatc gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct
     5461 tttccttctg tgtattatcc agactatgta gactttactt ttcattttat cattagttat
     5521 agataattga aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca
     5581 tctaaaagag gaggaaactc aaaaaaactt gcggcagctg tcagtagctg ctgcaagttt
     5641 tcgttgttat ttaagtaagg ctgatcagtc tgtttcaatc agtccgtatt ttccatcttt
     5701 acggcggtaa acgatacttg taccatttgt ttctgcatct tcaaaaataa agaagttatg
     5761 tcccagcatg ttcatttgta agactgcttc ttcactatcc attggtttca atgaaaggcg
     5821 tttcgtacgt acgatatcta attcagaatc attttcgttt tcgtcctctt cattgaataa
     5881 agcagcagca tctgcagtat tcatgcccgt ttcacgggat ttacgattaa tttttgtttt
     5941 gaatttgcgg atttggcgtt ccaatttatc aacaaccaaa tcgatacttg cgtataaatc
     6001 tggagaagtt tcttcggcac gtaatacaag ataaggtaat gggatcgtta cttccacttt
     6061 tgctgttttt tctgtataaa cttttaagtt cacatgcgcc gttgcatcgg gtgcgtcact
     6121 gaaatagcgt tctagtttgc ctactttttt ctcaacgtag tcgcgaatcg cttcagttac
     6181 ttcgatattt tcgccgcgta cattatatct aaacataaaa attacccctt tcgtctacca
     6241 caatcaagaa ggagaaggac cactctttcc ctcttcttaa ttctattata tcgaactcta
     6301 ggttatttgc aaattaaatc gctatcaaat ttccacatct aattcatctc gccaaagaaa
     6361 tggttcggat gattttcggc tgtgcgacct gcaaacaatc tgctgcgtgg aaaagagtct
     6421 gtcctgttgt atagacatca tcaataatca atattttttt attttgtatc ttagaaaagg
     6481 tgactgcaag ttcaaaaggt tgaggcatct ccaatctttc ctttcgcgtg ttttttgctt
     6541 gagccagcag atgcttctct cttttgagta gcatctgaaa aggaattctt gctgctttca
     6601 acatctcgct tacttgattg aatcctcttt ctgttcgcct tttttcagat aaaggtattg
     6661 gacaatagat gtatgtcggg tattcttttt ttatctgcgt ccatttatat gcaaaggtgc
     6721 ctgctagtcg gatatctccc ataaacttgt attgattgag ccaggccttc atattttcat
     6781 cgtacacaaa aaaggcacgg tgctgaaaat cataggtcgg atatttcttc ttccattctt
     6841 gacagtcact gcagtaaacg ggaatagact ttactttctt tttcgtactc tctagattga
     6901 aatggattgg tttttgacag ccagaacagc ctcttgtttc caataaatga aattgtgcgc
     6961 gacaaactga acaagtctga ggtaatctgt atttcgaaac accaataatc tcgctgaatg
     7021 ttaaattttg gaagatatct ttactgcagc aactgcacct cacagcatct tcttcccttc
     7081 ttcattcatt tctttgattt cctgacaagc ttttcgaata gctgcagttt tttcttcata
     7141 gaaaaagtat acttgactgt tcgtataagg ccctttacga tcggctcttc ctgcgatttg
     7201 taccaaagaa gatttagtaa aaaccggatg atccgctcct aaaacgataa cggatattct
     7261 ttcaaatgtg actccacgtt caaggatcat cgtggttaac agtatatcat attttgcttc
     7321 tcgcattttc tggacttttt ccgcccgctg attgtctctt gaagaaacat ctgtgatctc
     7381 atatctcgga agagcctctt gaagtgtggc tttcaaacga tgcatcatcg tgatacttgg
     7441 acaaaataaa aggatatggt tttctttaat taattgatga atgatccgca ccaaagattt
     7501 tatcttcttc ttgttcttgc atttttgtcg ccaattattc atgaaaacca agacgggttc
     7561 aggaagcaaa cgccgatgaa agcgtaaagc aagtttgtgg atgtcaaacg tctgcttgat
     7621 ttcctttagt aactttttat caggtgtggc acttaagtag atgaatcgtc cgtctggttt
     7681 aatcgctgtt tgcacagcat gagctaatcc tgcatccccg ttataaggaa acgcatcgat
     7741 ctcatcgatc actattaaat caaaggaccg ataaaaacgg tatagctgat gaatcgtaca
     7801 gataacaaaa gaagtaaagg tataagccgt tgctgtattc ccatggagca acgtgatttt
     7861 ttcttctgga aaaacataac gatagcgtaa aaacaattca ttacaaacat cgactcgcgg
     7921 tgtacaaatc gctactcttc ctccagcttt taaagtttcc actaaaatag gataaagcat
     7981 ctctgttttc cctgcacctg ttacagccca aataagatga tgtctttttt ctgaatggca
     8041 aagtattctc tttgagattt ccgtttgttt ttcagtaagc tgtcctgtcc aaaaaaagac
     8101 gacttctcga ggttttgctt caggctgatc aaacaaatat aaatcttgtt gagtgtcgca
     8161 tcgccctaaa tatagacaat gtgtacaaaa ataaccgtaa tctgcaagct gtccatcaat
     8221 gacggacgtc ctcattccac agcgatgaca aagaatccag ttgcccaaac gttccactgc
     8281 tgcgatcttc tcataatttg gtaaatttac ctcctcacag tctattgctt caatatctct
     8341 cgtcagaagc cttctacccc aaaaatgatt catattcttc gccctaaaaa aatatacgaa
     8401 aaaacaagcg atctattttt tggatcgctt gttcgatagt tatactgttc tatttattca
     8461 cctatgagcg tgattgcttg atctaacact ttttcgccat tcatcatacc ataatcagcc
     8521 atattgatga catctaaagg aatgcctttt ggttccagac gtttctcaaa atcagctttc
     8581 ataaagcgaa cttgtgggcc taataacaac acatccacgt ctttatttgc aaggttgtta
     8641 tctgcgtcag aagcggacac agcgaaaata tccgagtcga ttccttttgc ttcagcagct
     8701 ttttgcattt ttgttactag taaacttgtg ctcataccag ccgaacatac aagcatgatt
     8761 gtttttttag ccatactttt ctctcctttt taaaacaaac tatgcaaacg ttttcttcgc
     8821 gtctgttact ctattatata aaaagaaaga aatttgtcaa cgttttctaa acgcggttta
     8881 tttaatcaaa tagccaacta gatttcacta atttattgaa cacgacatat tctttgtcca
     8941 aattgatgac aactacttca tagcctgcat ttatccagcc ataggcacct ttttcagggt
     9001 gtttataatc atttgcccac caatctgtac tttcccttgc tgttttggga agtccgttac
     9061 tgggaaacag cagttcgtca aattgggtaa aggtcaacgt tacttgagag ccgccattgt
     9121 tcttcaaata atgcgtaatt gcatcgtact ttttcattcg ttcctgcatc cccagatcca
     9181 cctgcctttg tctttattat cgcatactac agcaattgcg caaccgttac agtgttgaaa
     9241 caacctagaa ctttatttat actgaataaa gaaaatgaaa aaaagaggtg acaagatgat
     9301 cgagcgctat cgtacaataa aagaaaatgg acaaagtgag attgagatca aaaaatcacg
     9361 ctttatctgt tctcttagac gaatagaatc agaggaccaa gcaaaaagtt tcattcaaga
     9421 aataaaaaaa gaacattgga aagcgaacca taactgcagc gccttcatca ttggagaaaa
     9481 aaatgaaatc cagcgaagca gcgatgatgg agaaccaagt ggaacggcgg gcgtaccgat
     9541 gttggaagtc ctcaaaaaaa atcaactgat caatgtagct gcagtagtca ctcgatattt
     9601 tggaggaacg aaactcggca caggcggatt aatacgagca tacactcatg cggtctctca
     9661 agcattgtct gacattggaa tcgtagaagg taccttgcaa caggagatct ctgtgatgat
     9721 cacgtatccg caactaggaa aattacagaa ttatcttgaa ctccaacatt tttttataaa
     9781 agaaatccaa tatactgatc aagttagcgt catctgtgct gtagatgaaa aaagtgctga
     9841 ccactttaaa gaatccatca ttgagctgct gaacggacaa gtccaatttg aagagggttc
     9901 tgtttcttat cacgagcaac tgataactga tcatatataa aaacaggtga ttctgattct
     9961 cttttatttc taataaggaa tcagggatca cctgtttttt atttttccaa ataaaactca
    10021 aaacgatttc ctacgtattg cgtacgcaca tactcaaacg gcgtaccgtc ttcaaaataa
    10081 gaaatctggc gcaaacgaag aactgcatct cctcgtttta cctctaaata gtcagcaatt
    10141 ttctcagaag ccaacacagc agtaatcgtt tgattcgctg gaccgatctt tttcccgcct
    10201 ttttcttcta gcgtgcgata caacgattca gtaatctctg ctttgctgta gccatcgatc
    10261 agcttttgtg gaacactggc aacttcaaaa caaataggga catcatctgc gaaacgaata
    10321 cgttccattc gcaagatcgt gtcatcttta ctcaagttta gtttttccat ttcacttgag
    10381 cttggcgaag tcaagaaata agagattgtc cgactagacg gtactcgatt ttgtgattcc
    10441 ataatttctg taaaacttgt tgcaccagac attttttctt gtactttttc tcgcgcaaca
    10501 tacgtacccg aaccgatctt tctttctaag attccttcat ctgccaagtt ttgaattgct
    10561 tgtctcaacg tcatacggct gacattgaat ttcactgcta attctctttc tgaaggaaga
    10621 cggtctccga ctttccatat accctgttcg atttcatttt ttaattggtc atgaatctgt
    10681 atatatacag gtaattgatt cgacataacg tacacccgtc ctttcgtttc ttctattata
    10741 actggtatat acctgattca caactgaaaa agataaaggt ttctatccat caacagagga
    10801 tttagacaca catcgttctc cctactccta ttgtcagaaa caatttttta gatatgttca
    10861 acctagaaaa taacaaaaga ccctaaaatc gatgaaaatg ccgacttatg gatcttttgt
    10921 tattattagc ggtttttagc catccatttt tttgtatgat tcgttgggcg ttttgatttt
    10981 acctggaccg cttttccgcc ttttttcgga atattcactt caatattgta tttttctgat
    11041 ttgaccaaat catataactt ttcagctgct tcgtctactt tttctttcca tttcatgtgg
    11101 atccctccaa ttcttgtagt taacttaact ctatcttaat tcagcagaat cttcaaggaa
    11161 caaggtagca aagcaaaaaa aagagctctc gaactaacg
//
LOCUS       DAZKMK010000090        11196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000090 DAZKMK010000000
VERSION     DAZKMK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.092"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1417
                     /gene="lysA"
                     /locus_tag="ACG8V1_002305"
     CDS             158..1417
                     /gene="lysA"
                     /locus_tag="ACG8V1_002305"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0330353.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1404..2204
                     /gene="dapF"
                     /locus_tag="ACG8V1_002306"
     CDS             1404..2204
                     /gene="dapF"
                     /locus_tag="ACG8V1_002306"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0330354.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2207..3088
                     /gene="dapA"
                     /locus_tag="ACG8V1_002307"
     CDS             2207..3088
                     /gene="dapA"
                     /locus_tag="ACG8V1_002307"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0330355.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3085..3801
                     /gene="dapB"
                     /locus_tag="ACG8V1_002308"
     CDS             3085..3801
                     /gene="dapB"
                     /locus_tag="ACG8V1_002308"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0330356.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3807..4502
                     /gene="dapD"
                     /locus_tag="ACG8V1_002309"
     CDS             3807..4502
                     /gene="dapD"
                     /locus_tag="ACG8V1_002309"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0330357.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4518..5519
                     /locus_tag="ACG8V1_002310"
     CDS             4518..5519
                     /locus_tag="ACG8V1_002310"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0330358.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5516..6877
                     /locus_tag="ACG8V1_002311"
     CDS             5516..6877
                     /locus_tag="ACG8V1_002311"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0330359.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6837..7988
                     /locus_tag="ACG8V1_002312"
     CDS             6837..7988
                     /locus_tag="ACG8V1_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0330360.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7963..9132
                     /locus_tag="ACG8V1_002313"
     CDS             7963..9132
                     /locus_tag="ACG8V1_002313"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0330361.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9307..10686
                     /locus_tag="ACG8V1_002314"
     CDS             9307..10686
                     /locus_tag="ACG8V1_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0330362.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10967..11196
                     /locus_tag="ACG8V1_002315"
                     /pseudo
     CDS             10967..11196
                     /locus_tag="ACG8V1_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3730 a         1952 c         2497 g         3017 t
ORIGIN      
        1 tttttataac catttttatt cttattttta aaaaaatatt tcgaaatata ctagacaatt
       61 cagaaaattt tgtttaatat tattccaata aaattcaaat ctttatcaaa tgaaattcca
      121 ataaaaacgg aaaaaaagtg aaaagaggaa tgaaaaaatg acagaattga taattggcgg
      181 agtcacagca agcacattgg cagaaaagta taagacacct ctgtatgtgt atgatgaaga
      241 aaagatgaga caaacgatgc tgtcattcaa gcgaggcttt gtatcgaaac gttttgatac
      301 aaaagtgctt tacgcctcta aagcattcca aacgattgag atgctgcatt tagttcgtga
      361 atatggattc gggctagatg ttgtcagtgg tggggaaatc tataccgctt tgcaagctga
      421 ttttcctaga gatactatct acttccatgg taacaacaag acgccagagg aactgatcta
      481 tgcacttgaa aatgatttgt tgcatattgt tgccgataat ttgatggaag ttgaattatt
      541 agcagaatta agccaacgct atttaaaaaa aatctgtatc atgcttcggt tgaatgtcgg
      601 agtagaagca catactcatg aatatatcgt cactgcgcat attgattcaa aattcggcat
      661 gagctatgaa agtaaagagt gccaacaagc ccttcagttg ataaaagaaa gcagctatct
      721 agagttagaa ggatttcatg ctcatatcgg gtcacaaatt tttgatgtta ctgcttggtt
      781 agctgaaatc gacaaattga cgaattactt aaccgatttt gaccaacctc tttccttgaa
      841 tcttggcgga gggtttggga ttcgttatac acaaaatgat cagccgatcc cagtcgaaga
      901 ggcactaaaa aaactgattg aatatacgga agaagctttg gaaaaacgaa aactgaccat
      961 tcgtcaatta ctgatcgaac cgggtagaag tattgttgga gaagctggta caacactata
     1021 cacgattggt ttcatcaaaa agacaccaca taaaaaatat tactttgtag atggcgggat
     1081 gacagataat atccgtccag ctctgtatca ggcagaatac gattgtgatt tagcagcaaa
     1141 actgtcggaa gaaaaaacag agaaagtgac gattgcagga aaaatgtgtg aatcaggcga
     1201 tgtggtaatt aaagaagctt atttgccgaa agcagaatcg ggtgatcttt tagctgttta
     1261 ttccacggga gcttatggat actcgatgag cagcaactac aatcgagcga cacgaccagc
     1321 agttgttttt gtcaataatg gccaatcaag actgattgtt cgcagacaag cattttctga
     1381 tctactaagg ggggaagttt cctatgatcc ttcataaata taatggctgc ggaaatgatt
     1441 ttatcttatt agattacgca gaagacacag attatagtca attagccgct tctttatgca
     1501 caaaagaaaa atatgatata gacggattga tcgccgtaaa aatcgatccg ttggaaatga
     1561 tctattacaa caaagatggc agccgcgcgc cgatgtgcgg aaatggcatt cgctgttttg
     1621 ctcgatacgt aaatgatcag aacatgatga atgaacagga attcaacgta gagacattag
     1681 caggcacaag aaaaattacg gtgttggatc cctcaccttt ttattgcagc gtagatatgg
     1741 gagtgccaga tgattctccg gaaaaaatca aagctaatca aacttatcca ataaaaaacc
     1801 aggaaataga gatcaatggt gaaaaagtaa cgatcactag tttattttta ggaacgattc
     1861 ataccgttgt ttttgttgaa aatgcgcgtg aggaagtctg gagaaaaaga ggtgaactga
     1921 tctgtcatca tcctttgttc aaagaaaaaa ccaatgtcaa ctttgtagaa gtagtcaatg
     1981 aacaagaatt gatcgtccgt acctatgaaa gaggagtagg ttggacactt gcttgcggta
     2041 caggttgctg tgcctcttat gtggttgcta aggaaactgg gaaaatttca gcagatcagg
     2101 tagttgttca cttggaacaa ggagacttga tgatctctgg tgaggatgcg atacaaatgg
     2161 ctggcccagc agttcatgaa tggacaagaa tattggagga atgaagatga taaaaggttc
     2221 gatcgtggcg ctgatcacgc cgatgaatga agagggatca gtagattatg ctggtttgga
     2281 aaaattgatc caattccatt tagatgaaca aacagatgga ttattagttt taggtacaac
     2341 aggagaatct tctacgctca cacaatctga ggaggagcag atccttcaac tgacagtaaa
     2401 gaaagtggct ggccgtgtgc cggtgattgc aggagctgga acgaataata cgaaagaaac
     2461 gattgaaaaa gcgaagcatt ttgcttcttt gggggcagac gcacttttgg tcatcacccc
     2521 ttactacaac aaaacaagtg atgcaggact agctgctcat tttacagcaa ttgcagaagc
     2581 ctcaccgatt ccgcttattt tgtataatgt tccttctaga acaggcatgt cgattccaat
     2641 ccatgtatta gtaaatttag cagaacatcc taatatcatt ggtttgaaag aagcctcagg
     2701 agatatggct tatgtgatgg atgccgctcg gttgattgga gaagaattct tcctttacag
     2761 tggaaatgat gatttgatcc taccagtaat gagtgtagga ggaagcggtg taataagtgt
     2821 atgggcgaat atccaaccta aaatagtcca tgaattagta aaagatacgc aggacggaag
     2881 atggcagcaa gcaaaagaaa agcaattaaa tgcacttgaa ctgatccatg ccttgttcag
     2941 tgagaccaat cctattcctg tcaaagcagc catgtcatta ttagacttac cttccggacc
     3001 tctacgcctg cctttagttt ctctttcaaa agagaaaaag aaacaattag ctcagctttt
     3061 gttaaaagaa aggacggttg aaaaatgaaa atcggactga ttggcagtgg acagatggga
     3121 aatagagtcg aagaagttgc acaagaaaga ggagatacgg tccttctttt acggacggca
     3181 cctaaagaag caactacgct tgcttttacg gaactgccag aactgcttat tgatttttct
     3241 catccagata atttagagaa aatcctttcg tatagcttat catatcaact tccgttagtc
     3301 attggcacga caggttatac acaatcacag ttccaagaaa taaaagaaca tgccaaacat
     3361 gtacctgtga tgtatagtgc taatttttcg ttcggcatca tggtgatgaa tcaactaatc
     3421 aagcaagcag ctgcaatgtt gcaaggctgg cagatcgaac tgattgaaaa acatcatagt
     3481 cgtaaaaaag atgcaccatc tggtactgca aatatgctgc ttcaaacaat ccaagaagtg
     3541 caaaagctgc agcccgtata caactggcaa gggaaaaaaa gagaagaaaa ccaaattggt
     3601 gtacacagta ttcgtgcagg ttctttacct ggtgaacatg atgtattgtt tgctgctact
     3661 gatgaaatat ttacgatcaa acatgaatct ttttctaatc gaatctttgc tgcaggcgca
     3721 gtagaagcag cggactggtt gaaagatcag tcaccaggtt attacaaatt agaagattta
     3781 ttaatggaca aaggggtgta aaaaacgtgg aagctcaaga aatcatccgc tacattcaaa
     3841 cggcatctaa aaagacgcct gtgaaagtct atctgaatgt aaccgagcca atcgaattta
     3901 aagaaagtaa agtttttggt gaaggaagca gcttcgttgt ttttggtgac tatgagacga
     3961 ttgctccggt tttagaagct gaatcagaaa aaatcattga aatcgaaatc gaaactgctg
     4021 caagatacag cgcagttcct ttgctagata ttaaaaaaat taatgctcgt atcgaaccag
     4081 gtgctattat cagagatcaa gtgtcaatcg gcaacaatgc cgtgatcatg atgggagcga
     4141 ttatcaatat tggtgcagta atcggtgaaa acacgatgat tgatatgggt gctgtcttag
     4201 gtggacgagc gacagtgggc aaaaactgcc atattggtgc aggagctgtc ttagcaggtg
     4261 tgatcgaacc agcatcggcc aaaccggtta tcgtagaaga cggcgtactg gtaggtgcga
     4321 atgcagtgat tgttgaaggt gtccatatcg gaaaagatgc agtcgtggca gcaggagcag
     4381 tggttttaga agatgtggca gcagaaactg ttgtcggtgg aatacctgca cgtgtattaa
     4441 aaataagtga tgatcagaca aaagaaaata cagcattgat tgctgcgtta cgcgaattat
     4501 agaggagatg gagaaaaatg cgcgaaatag atacctgtat tgtgggggca accggattag
     4561 tcggccaaac gatgctcaaa gtgttagaag aatatgattt tccagtagga cgattgaaat
     4621 tattagcttc aaaacgttca gccggtaaag tttgtgtatt caagaaaaag aaacacactg
     4681 tacaggaatt gacagaaact tcatttgaag gatatgattt agcgctattt tctgcaggtg
     4741 cttctgtatc caaagagttt gcgccaatcg cacgaagtca gggactattt gtcatagata
     4801 attcctcagc atggcgggaa gattcggata ttccattaat cgttccagag gtcaatctgg
     4861 aagactatac attgaatcgg ctgattgcaa atccaaattg ttcaaccatt caagcggtac
     4921 ttccgcttaa agctttacaa gaagcctttg gtatccagcg agtcaattat tcgacctatc
     4981 aagcagtttc tggttccggg caaaaaggca ttgaagatct tcatcgtacg caaaatgacg
     5041 aggaaccagt tttgtatcct tataatatta gccaaacggt gattccggaa atcgatcagc
     5101 ctttggaaaa tgggtataca aaagaagaac agaaaatgat aaatgagacc cgtaagattc
     5161 tgcatcagcc tgctttgcca gtatcagcaa cctgcgtacg tgtgccaatc gaaaatggac
     5221 atggagtctc tgtggcagtt caactgcaaa aagagtttac gacggaagaa attagagagt
     5281 gtctcagcca gtttccaggg attcaattag aagatgatct agcagcacat aaatatccga
     5341 cgagcattct tgctagagga acagatatgg tttatgttgg gaggatcaga aaagacactt
     5401 ctttagagaa cggattgttg ttctatacga ctgccgataa catacgcaaa ggagccgctg
     5461 cgaatgccgt acagattgct gcagctttaa tgcagaaaat agcggaggtg gcaagatgag
     5521 taatacgctg aaagtagcga aattcggcgg aacatcgatg tctcatgaag agcaattcaa
     5581 aaaagtccgt acaattgttc tttcagatcc caatcgccaa gtgatcgttg tttctgcttt
     5641 aggcaaacgt acgtcagatg atgacaaagt aacagactta ctttatttga tttatgctta
     5701 ccttcagcat catgttgatt ggacacccct ttggaaaagg atttgtgatc gtttcattca
     5761 agtaagagat gttcttgccc tgcagttacc tattgaagat gaactagcac gcatacagaa
     5821 acaactggaa gacgaaaaga taactgaaga ttatttagta agtagaggtg aatatctgac
     5881 tgctcaactg atggctgagt atcttggtta ccagtttatt gacgcaaaag acctgatttt
     5941 ctttgactat gatgggaaaa tcgatcagga aaaaacagca caggaaatca caaaagcatt
     6001 taaaagatat ccgaaaatgg ttgttccagg gttttacggg gcgaaccctg caggaaaaga
     6061 aaaattattg ggccgtgggg gaagcgacat taccggcgcg atactggcga agatgttgca
     6121 agcggaaagt tatgagaatt ggacggatgt ttcaggaatc atgatggctg atccgcgaat
     6181 catcgatcat cctaaaatca tcgaggaaat ctcttttaga gaactgagag agatggctta
     6241 tatgggggct aacgttcttc atgaagcagc tgttttccca gttcaagagg ctgatatccc
     6301 catccaaata aaaaatacca atgatccttt cgctcaaggg acaaaaatat cgaatcacga
     6361 aatagaaaag gaaaatggac tgacaggaat agctggacgc aaggattttc tttccatcac
     6421 tttattcaaa cgtcatatgt ccgatgaaat cgggtttatc tggaaagcaa tgagcatttt
     6481 tgctaaacat gagatatcaa tcgaacatat tccttcaggg atcgacaata tcggagtgat
     6541 cgtctcagca gaagcgattg ccgataagtt atttctcatc acgaaagaat taaaagaaac
     6601 acttggcgtg gaagaaatcg tcgtgatcga agacttagct ttgatctctg tcgttggggg
     6661 tccgcataaa gaattgattg gattatctgg gaaagtgtta tcgatattga acaaattaga
     6721 aattcgcaca tcgatcttat cacaaggagc acaggagttg aatctgatca tcggtgtacc
     6781 gaataatcaa tatgaaacag tagtgaaggg tatctatgaa gggatggtga atacggatgc
     6841 tgaaagaata tcgaatggca ctccatcaaa ttcctgaaat cgggtttcat gaattcaaga
     6901 caaaaaaata tttatatgaa caagtaaaaa acagcgaggc cgttatccat gaaatcggcg
     6961 aaacaggact actcctttat tttgataatc agcaagaaga aacgattgct tttcgaacag
     7021 atatcgatgc actgccagtc aaagaagaaa cgaatctctc ttttgcttct aagcatgaag
     7081 gttatatgca tgcatgtggt catgacggac atatggcgat gcttttaggg ttgactgact
     7141 ttataactaa acacattgag gatcttattt ataatgtagt actgatctat cagccatcag
     7201 aagaaatttc cggtggcgca caaagcgtta ttcagtcagg attgttggat aaatatgaag
     7261 tacaagcaat atttggcttt catctatggc ctggactagc taagggagaa gttttctctc
     7321 gaccaggacc tttgatggca caaagcagtg aaacagacat tatcattaaa ggccagtctg
     7381 cacatattgc ttctagtgaa aaaggaatcg atagtctgga ggcagctgta cgatttttga
     7441 agaatgttta tgattttgac cggcaattac cggcagagtg gaatcatcta atgaaatttg
     7501 gccaaatcca aggtggaacg atccgaaatg ttttagctgg agaagttatt gtatccggaa
     7561 gtatccgttc ttacagttca gatatgcaaa attatttgaa gaggggatta gaccatttag
     7621 cagaacagtt tcactctaca gctactattt cttttcgcta taatgacggc tatccagctg
     7681 taatcaacca tagaggctta tatgcagcac tgttagattc aaacaaacta catgaattag
     7741 atgctccagt tcttcaagcc gaagattttg gcatatatac gcaacaatat ccatgtgtat
     7801 ttttcttttt aggtgtcggt gatacccctg ctttacatga tccgacgttc gattttgata
     7861 tggatgtttt agaaaatggt gtagaatggt acaaaacgat tctttttaca gaaaatcttc
     7921 ttgtaaaaat cgatagcagt tcaaaagagg agcgagtaaa taatgatgaa tttatccaat
     7981 cgatttagtc ctaggctttc tagaatagaa gtatcaaaaa tacgacaatt cgatcagcaa
     8041 atatcgtcga taccagatgt gatcaaactc accctgggag aaccggattt tccaactcct
     8101 gaacatgtca agcaagcagg aatagcagcg attgaagaag acttttctca ctataccgga
     8161 atgagaggat tagaggaact gcgggaagcg gcttgtatat ttcagcaaca gcgttatgga
     8221 ttaacttatg atccgcaaac ggaagttttg acgacagttg gtgcaacgga agcgattgcc
     8281 tctgcgttgt tatctgtttt ggaagaagga gacaaggttc ttattccagc gcctgcttat
     8341 tcaggatatc agccattagt ggaactagct ggtgctgaat tgatcccgat agatacttct
     8401 gacacagggt ttgtctgtca gccggaacaa tttgaacgag catttgaaca atatggatca
     8461 gctgtcaaag cagtcatttt aaactatcct aacaatccta ctgggacgac attgtctgca
     8521 aaacaattga aagcaatcgc tgaggtttta aaaaaatatc cggtatttgt tataagtgat
     8581 gaagtatatg ctgaattgac ttatagtgga acgcatatgt ccatagctac ttatcttcca
     8641 gaacaaacaa tcgtgataag cgggttatcc aagtcacatg cgatgactgg ttggcgagtg
     8701 ggctttattt ttgctcaaaa agcccttatt gacgaactaa ttaaagtcca tcaatatctg
     8761 gtgactgccg ctacaacgat gtcgcaaaaa gctgcggctg aagcgctaat aaatggggtg
     8821 aaagatagcg agaatatgaa agagcaatat caaacacgaa gagactatct ggtccagcaa
     8881 ttggcgcctt taggatttga agtgacccag ccaaacggtg cattttactt gttttgcaaa
     8941 ctcccagctg ccattcaaac tgactcttgg cagttttgtg tagaattagc ggaacaggca
     9001 aaagttgcgt gtatcccagg aattgcattt ggcccagaag gagaaggtta tatccggatt
     9061 tcttatgcta gcagcatgga aaaattacat gaggcctgca aacggatcaa agacttttta
     9121 acagaacagt gattcataga ttttatcaaa aaaacaatcg ttaattttgc ggtatattct
     9181 gcaaggagac gattgttttt taaatttagt tcaaatgaac gcgtttcttt tgattcataa
     9241 ctaatgtaat gttaagttta aattgtttag aatgatttta aaagaatatt caaggaggtt
     9301 tcatcaatgg aaaaaatcaa agttgtcatt gtaggtgcat ctcatggagg gcatcaatcg
     9361 atcttggaac tattgacacg atacggtgaa aatgtagata tcacgctatt tgaagcagga
     9421 gactatattt ccttcatgtc atgtggtatg gagctgtatt tagaagacaa ggtaacagat
     9481 gtaaatgatg tacgtaattt cagaccagaa aactttccgc aatccaatgt tcatatttta
     9541 aataatcacg aagtaaaaac gatcgatgcc gataaaaaaa cagtaacggt tactagcgca
     9601 tcagataacg aaacaaaaga atatccttat gacaaattga ttttgagttc tggtgtcaaa
     9661 ccaaacaatc tgccagtacc aggagcagat ctagaaaatg tctacttgat gcgtggatac
     9721 aattgggcaa ccaaaatcaa agaaaaatta aatgatccgg ctattaaaaa aatcgctgtc
     9781 gtaggtgcgg gttacattgg gatcgaagca gcagaagcaa gccgaaaagc tgggaaagaa
     9841 gtcgtattgc ttgatgtgat cgatagacct ttaggcactt atttagatgc tgagatgaca
     9901 gatattttag aacaacacct aaatgaaaac ggtattgaag ttttgacaaa tgcaaaaatc
     9961 gaagctttta ctggtaacgg aaaagttgag gcaatcaaaa caagtgaaaa agaaatccca
    10021 gcagatctag tcattcaagc tgccggtgtg aaggcaaata cagaatggtt aaaagggatc
    10081 gtcgacttag atgaacgtgg ctggatccaa acaaatgaat acttgcaaac gaatcttcca
    10141 gatgtgtatg cagtaggaga tgctacactt gcttattcta tcccagcacg caaaaaaatg
    10201 ccgatcgctt tagcgactgt tgcacgtaga gaagcacgtt atgtagtaaa acatcttttt
    10261 gaagaagtac caaacaaacc gttcggcgga gtcgtaggtt cctctgcatt aagtgtcttt
    10321 gactatcatt ttgcagcaag cggattgaat agctttacag cagcaaaagc aggagtagat
    10381 gtacgagtgt cttattatga agatacgtta cgaccaaaat atgtaccaga agaacaagga
    10441 aatccaaaag tgtccatcca attattctcg gatccaatga cccatcaatt gctaggagga
    10501 gcagtattat ctacctatga tgttacaggt caaggaaatg tattggcact agcgatccaa
    10561 aacaaactga ctttagaaga actagcagaa gctgacttct tcttccagcc aggctttgac
    10621 cgacaatgga gcatgttgaa tctagccgct caacaagcgt taggagaaga accatttgta
    10681 gaataagaaa acgaaatatt tgaaaagagg ttgtgaaaaa gctgccttgc atcaagtaat
    10741 aagaacagcc aagtaaaaac agcttcttat gtttttaact tggctggact tattaccgca
    10801 gatgcaagct tttgaacacc gtttattttc aaaaaaggga ctgtgacacg atttgtgtac
    10861 cgacccccaa aagttagacc aaaaaatcta acttttggag gtcggtattt tttatggtta
    10921 aatatacttt tgaacttaaa ttaaagatcg ttcatgatta tttggatggc aaaggcgatt
    10981 ggaccgtaaa aatccagatc aagagattga agcggaaatg cttaaaattc gggagaagca
    11041 caaggattat ggctgtttac ggatgaccaa tgaactaaga aaccgcggtt tctctatcaa
    11101 taaaaagaaa gttcagcgct tgatcaagaa gctaggaata aaagtaacgg catacacgcg
    11161 taaatcacgt cgctacaatt cttatcgagg aaaagt
//
LOCUS       DAZKMK010000091        11187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000091 DAZKMK010000000
VERSION     DAZKMK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.093"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..533
                     /locus_tag="ACG8V1_002316"
     CDS             <1..533
                     /locus_tag="ACG8V1_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330363.1"
                     /translation="SFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILT
                     LAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSN
                     RRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFS
                     FAREDEDNPKTYDILA"
     gene            complement(594..959)
                     /locus_tag="ACG8V1_002317"
     CDS             complement(594..959)
                     /locus_tag="ACG8V1_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0330364.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1035..1280)
                     /locus_tag="ACG8V1_002318"
     CDS             complement(1035..1280)
                     /locus_tag="ACG8V1_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0330365.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1350..1604)
                     /locus_tag="ACG8V1_002319"
     CDS             complement(1350..1604)
                     /locus_tag="ACG8V1_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330366.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1768..2091)
                     /locus_tag="ACG8V1_002320"
     CDS             complement(1768..2091)
                     /locus_tag="ACG8V1_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330367.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2278..2351
                     /locus_tag="ACG8V1_002321"
     tRNA            2278..2351
                     /locus_tag="ACG8V1_002321"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2312..2314,aa:Ile,seq:cat)
     gene            complement(2736..3233)
                     /locus_tag="ACG8V1_002322"
     CDS             complement(2736..3233)
                     /locus_tag="ACG8V1_002322"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0330368.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3273..4082)
                     /locus_tag="ACG8V1_002323"
     CDS             complement(3273..4082)
                     /locus_tag="ACG8V1_002323"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0330369.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4416..5273
                     /locus_tag="ACG8V1_002324"
     CDS             4416..5273
                     /locus_tag="ACG8V1_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0330370.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5266..6168
                     /locus_tag="ACG8V1_002325"
     CDS             5266..6168
                     /locus_tag="ACG8V1_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0330371.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6201..6551
                     /locus_tag="ACG8V1_002326"
     CDS             6201..6551
                     /locus_tag="ACG8V1_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0330372.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6600..7523
                     /locus_tag="ACG8V1_002327"
     CDS             6600..7523
                     /locus_tag="ACG8V1_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0330373.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7601..8494
                     /locus_tag="ACG8V1_002328"
     CDS             7601..8494
                     /locus_tag="ACG8V1_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0330374.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8532..9095
                     /gene="amaP"
                     /locus_tag="ACG8V1_002329"
     CDS             8532..9095
                     /gene="amaP"
                     /locus_tag="ACG8V1_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0330375.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9109..9297
                     /locus_tag="ACG8V1_002330"
     CDS             9109..9297
                     /locus_tag="ACG8V1_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0330376.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9310..10212
                     /locus_tag="ACG8V1_002331"
     CDS             9310..10212
                     /locus_tag="ACG8V1_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0330377.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10244..10486
                     /locus_tag="ACG8V1_002332"
     CDS             10244..10486
                     /locus_tag="ACG8V1_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0330378.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10742..11113)
                     /locus_tag="ACG8V1_002333"
     CDS             complement(10742..11113)
                     /locus_tag="ACG8V1_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330379.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3954 a         1921 c         2128 g         3184 t
ORIGIN      
        1 taagcttttt taaagaatat ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa
       61 gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
      121 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
      181 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
      241 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
      301 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
      361 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
      421 ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
      481 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
      541 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
      601 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
      661 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
      721 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
      781 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
      841 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
      901 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
      961 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
     1021 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
     1081 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
     1141 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
     1201 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
     1261 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
     1321 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
     1381 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
     1441 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
     1501 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
     1561 gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa
     1621 cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa
     1681 ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca
     1741 ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa
     1801 gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat
     1861 cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt
     1921 tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat
     1981 tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc
     2041 aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa
     2101 ataaaagaat aacatttttt ctaaaataag caaataaaaa ttaaagtaac aaaatagtta
     2161 tgacacaagt aaaagaaata agaataacaa tattatttta cttatatttt gaaacttgtg
     2221 agagtttgtc attcgtgcta tagttgaaaa gtaacaaaaa taaattttat ttatgatgta
     2281 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
     2341 tacaaggtac ataagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     2401 gtaaaaacag cttcttatgt ttttacttgg ctgttcttat tactgcagat gcaagctttt
     2461 gaataccgtt taaggaagag gttgtgaaat cagcgttttg acctgagtat tagcttaaca
     2521 aacggaaaaa tagttcctca tattttgccg tttgttaagc taataccgag ggtcagctga
     2581 ttgaacacca tttaagaaag aggtcgtgag cctgccgttt cactctgtgt cataaaaaag
     2641 ccaatctact ttgatagaaa aagcacgtag gactaaaaca gttctgcgtg ctttttttat
     2701 tgtgtgttaa atatcgtaaa tgaagataga tatttttatg attcgacgat agagacgttg
     2761 aaatctttca acagctctaa agcggattct ccgtgttttc tactggagac taacattgga
     2821 tcaacaaaaa agtctgcttc aggaaaagca ctctgcaggc ctatcagatt agagagtaca
     2881 cacaagtttg tttcaatccc gactacttcg atctcttttt gttcttcttt gctttcaaat
     2941 aaagtttcct ggatttcagc taaagtttca ggtggaatcg taaagtaata ctttttaatc
     3001 actcgttcat tttcttgtgg agaaagaaga gggatcaatt gcaaatcttc tcgttcttct
     3061 tctttgtcct tcagttcaac tggtatatcc cgtgtgaaga gaatatattc attattttgg
     3121 cgtgctttag ctaatctttg agcaatacgt tctgccaatt gatctgactc tggaatataa
     3181 aattcgctgt ttggatcaag aatatgattt tgcatatcaa taataactaa cattttcttt
     3241 cctcctacat tcatatcgtc attttattct ttttaataga agttttttaa aatataagca
     3301 acaccagctt cttcattcgt cagtgttact tggtttgcaa tttcttttac ttgtcgctgt
     3361 gcattcccca tcgcaatact caacccgctg taattcaaca tagcaatgtc gttttcttga
     3421 tcgccaaaag ccatgatgtc actcgattcg attccaaggt aatccgccag aaaaacaagt
     3481 gaatgagctt tagtgatacc tgtaggcgtg atttctaaga aatgcggatc agagaatact
     3541 gattggatac catctgcttg cagatctctt tgtagcttct gtagtttttc ttcactctta
     3601 tctgttaatg ctaatttaag atagctaaga gaaggatctt ccaacatatt taaaatttga
     3661 tcaaaagaat ggttctgtaa attgaaatgt tcataataag ggtcatcttt gactagattt
     3721 gattcatcga atgtcagctg atcatcttgg gtttctcggc tagcaatgat ggatattttc
     3781 gcttcttgag catagaagaa ggcttttctc agtgtatcgc tatctatttt tgaacgtctg
     3841 agaactttcg ccatttttcc atccttcatt tcagcaatca gtgaaccatt ggaagaaatc
     3901 aaatagccac cgattcctaa ttcggaaaaa atatctttag cagaaatgct gttccttccg
     3961 gtagcgacgg ttacgattcc gccattttca tcaaatttcc gaagtgcttg gatattttct
     4021 tctggtatct ttccatttcg atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc
     4081 attttatctt cctactttct ttttacatat ctattttcct gttaccttaa gattaaatga
     4141 gaatttaaaa aaatgaaact aacttgcttt gtcattatga attttttcaa aaaacattct
     4201 taagaaaaag gtggctatat atagtagttt ttggtcaata cctttgaata aaaagccatg
     4261 aaaaacgaat agttagcgct taccgatgcg ttttgtgata aaaaaatttt aaaatattca
     4321 atgaaaacaa agcatatatt ttgctttaaa accaaatgag gaatattcta gatgtagaac
     4381 aggttagaaa aagattagaa aaaggagtga ttttgatgga gaagagatac ggcgtagttc
     4441 ctgtggtaac tgcttcagac gaaaattatg ctccttactt gagcgtgatg atcgcaactg
     4501 cattagaaaa ttgcaataaa acaagacgca taaaatttta tgtgattgac gatggtctat
     4561 cagaatatag taaagaagga ttggaagaaa cggtaaataa atattcaagt aatgcaagta
     4621 tccaattcct gacagtggaa aaagatatct acgaagactt cctagtcagt gaccatatca
     4681 caactaccgc ttacttaaga atttctttac caaacttatt agctaaagag gattataaga
     4741 aagtccttta cttagattca gatgtgttag tactagacga tatcgtaaaa ctatatgatg
     4801 agccgttaaa tggaaaaaca atcggcgcta ttatcgatcc aggccaagtg aaagctttgg
     4861 aaagattagg aatcgattcc gatgacttgt acttcaattc aggagtgatg gtgattgaca
     4921 ttgatcaatg gaataaaaaa gaaattacag aaaaaactat ccattacctc agtgaaaatg
     4981 gcgatcgaat catctatcac gaccaagatg ctttgaatgc tgtactttat gaagattggg
     5041 aacaacttca tccaaaatgg aacatgcaga cttcgctgat ttttgaacgt catccagcac
     5101 caaatgaaaa atatgaacgt cagtataaag aaggaaatga aaaaccttcg atcgttcact
     5161 ttacaggaca tgataaacct tggaatacgt tgaaagatca tccgtatacc aatttatact
     5221 taaaaaaatt agcacacagt actctaacga aagtaggcga gatcaatgaa taaaaaagaa
     5281 atagcagtag tcgcaagttg taacacaaaa ttcgtaccac atttagctgc attatttgtc
     5341 tcagtattag ataattgcaa cccgtctaaa tttgttcggt tctatgtgat tgacgatgat
     5401 atcgattttg aaagtaaaca gcttttacgc ttctcagtga aaaacgcacg aatgaactca
     5461 gatgtggaat tcttgaaaat caataaagaa ttctttacaa atgtggtaat cagcgatcgt
     5521 attccagaaa cagcttatta tcgtattgcg attccggaat tgttccgcgg cacagaagtt
     5581 gaacgtatct tatatatgga ttgcgacatg atcgctttac aagatatctc taaattatgg
     5641 cgtcttgact tcggtgattc aatcgtagca gctgttgaag acgctgggtt ccatcaacga
     5701 ctggaaaaaa tgaaaatttc agctaaatca atgcgttatt tcaactcagg tttgatgctg
     5761 atcaatgtga aaaaatggtt agatgaaaat attacgcaaa aagtattaga tttcatcgaa
     5821 cataatccag aaaaattacg attccatgac caagatgcat taaatgctat cttacatgac
     5881 cgttggttac cacttcatcc gcgttggaat gcacaaggct atatcatggc aaaagcaaaa
     5941 aaacatccaa cagcagcagg tgaacgtgaa tacgaagaaa ctcgcaacaa tccttacatt
     6001 atccattttt caggacatgt gaaaccgtgg agcaaggatt ttgaagggcc aacgaagaaa
     6061 tattacgaaa aatatgcagg gatgacggct ttccgttgtg tagctaaatt ccctaaatat
     6121 ccaaaatatg caaaagttca accaaaaaca gatgtagttg ttaaataata gaagacaaca
     6181 actgaagagg gggagtagga atggaaatta caatgtctaa aaatgcagta gagacgttaa
     6241 ttgaaaaggt tggggaaaat acaaaaatcg cactcgcgct tatcaatgat tccgatccat
     6301 ttctaaggga caaaggtgct tttgcaaaag gcagcttttt tcaaatcatt ccttttgttt
     6361 cggaatttgg agaatatgcg actaaaatcg agcatccatt attggacatc tatacatcta
     6421 aattagagca aaattatttc ggtaaacgat tgaacatgga cttcaacaaa cagcttgaca
     6481 gcttttcatt agaaaatgaa atcgcagtac tcgattataa tatcaaatta aagaactgct
     6541 tcttcagcta atagtttcga gaaagtccaa aaaggaggta tacattgaga tactactttg
     6601 tggatgaaga aaagaatgta atagaaacag atgaaaaaaa atataattgg ctcgtacttg
     6661 atagtgcaaa gcccgaagag attgagcaag caatcgaaga attcgagctt cctgatgata
     6721 ttttcgtagg aaccgaatat ccggaagaag tttctcggat ggagcgcctt tatgagacga
     6781 aactagagca tccattttct ttggtagtga ttaatctaga tcagtcagat aatcgtatcg
     6841 aaaaaaaatt aacgccaata tcttttgtac tttccgataa tctcttgatt acttgtatcg
     6901 atagaaaaac cgaatttgtg gatcggctgc tgacaaagca tggaaatcat ttaaactcgt
     6961 ttgaaaagat catcacgtat acattattag atatttacac ccattacgtg aaagaactaa
     7021 gagagatgaa aaagcgaatt gacgctttgg ataaagcagc tcgtaaaaca acggaaaatg
     7081 aagaactgta taaacaggca gatcttgaaa gagacatcgt atatatcgat cataccttaa
     7141 gagatcaacg ggaaacaatg gatcaattat ggaattctga aactttcatc aaacgtttag
     7201 acgatgaaca gctactctat gatgtccatt tgagacagcg acaaacagaa aaaatgatcg
     7261 agatctatcg agatttatta gagagtattg gtgatctatt caacgggatg atggacaata
     7321 atctaaatca tatcatgaaa tatctagatt cagctgcatt aatcatttcc gttccggcgt
     7381 tgttcgcagg gatttgggga atgaatacag gaggattacc gggtaaaagt tctgctctag
     7441 gctttatact cgtagtcttt ggctctttga tagcagcagt agttttaggt taccatttat
     7501 accggaaaga ttatacaaat taaagaatct atttaaaaca ggtatcaaac aaagaaattt
     7561 gttttggcaa tacaatttaa aaactaggag gaatacagca atgacagaac caaaattaat
     7621 cgacccgcga aacttgtacc atacggagaa atttcctaaa caagatcagg atacaccagc
     7681 tttacagcag aatatgattc ctgttcctga ttgtggagaa gaatcctatg aaggcaataa
     7741 tcaactagaa aaccgccgtg tactaatcac tggtggagat tcagggatcg gtcgtgcagc
     7801 agcgattgca tttgctagag aaggggcaga tatcgctctt caattcttcc caggagaaga
     7861 aaaagatgca gaggaagtag caaaatacgt gaaagttgca ggacgtaaga ttgtcttact
     7921 accttttgat cttcgtgacg aacaagcacc taaagaaatc atagataaag ccgtgagtga
     7981 acttggcggg ctagatacga tggtactaaa cgcaggacag caaatttctt gtcagtctat
     8041 tgctgagtta ccacttgagc aagtcaaaga cacatttatg ataaacatca tcgctatgtt
     8101 tgctttagtg aaagaagctg ttccgcatat cccagcagga ggagcaatcg taacaacaac
     8161 ttctgttcaa gcattcaatc caagcgaaca tttattagat tacgcagcaa caaaagcttc
     8221 aatcgctaac tttacgatcg gtttagcaaa acagcttgca cctaaaggaa tccgtgtaaa
     8281 tggtgtagca cctggtccaa tctggacacc attacaacta gacgaaggac aaccttctga
     8341 ttcgatccct gaatttggtc agcaaagttt actagaacgt gcaggtcagc caggagaatt
     8401 agcaccagtt tatgttttct tagcatcgaa taaagcaagt tatgttacag cccaaattta
     8461 cggagtaact ggtggagaag cgatcaactt ataagaagtt gatcgtttca cccctatgag
     8521 gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt gatcggaaca ttacttttgc
     8581 tatctgtgct tttgtttgcg atactattaa gtgcaaccta tccattgcct tttcagctag
     8641 agaagtttcg attcttttcc atcactaatt catacgtgca acaatatgta ttttggcttg
     8701 ccgttgcatt tgctattttg acaattatca cgttacttgt tttcctgttt tatccaaaaa
     8761 caatcggaaa ctttctactg aaacatggtg atggggagtt gacgttggat aaaaaagcaa
     8821 ttgaaggaat ggttcgttct cacttgcatg aagatgaatt catccattca ccaaaagtaa
     8881 atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa aggggagttg aaacgaactt
     8941 cagcactcat tggtaaaaca ggaacattga tgaaagaaat cgaaaatgaa gtacaacaca
     9001 tattgggaac aaaagagcca gtgaaagtat cggtgaaata cacaggcttt gaagaaccag
     9061 ctaaacgaag caatgaccgc aaacgagtag aatagggggg gaccaaaaat gaaagaacta
     9121 ttttcgacat acaagctccc tatcatcttt ggggtacttg gattgatatt agccgtttta
     9181 tggatcaccg tgggcttttt caagacctta ttgatcttga tcatggcagc gatcggcgtc
     9241 gctatcggtt tttatctgaa acaaacgaaa atcttagatc actattttaa atcttaggag
     9301 gaaacatcca tgaataacaa taacaaaaat aataacaata aaatgaacaa ccaaaaacca
     9361 gtgtctgcac atccagaccc aacgacacca aaccaatcaa atggtgtgaa cgatgaaaag
     9421 aacaaaacag caccagtatc tgcacatcca gatccagtag tccctaacgt tcaacaagga
     9481 gtagaagaga agaaacatga tgcagaaaag catgcacctc atcatccagg aactccagaa
     9541 tctaaagttc cagaacaaca tgatgcaatc gatccttcta aacctgtgga agagcaaaaa
     9601 catccttctg accatgcgaa agcacaagaa aaacttagtt ctgaccacac aaacggacaa
     9661 ggaaaacttg gttctgatca tacaaataac caaggaaagc atgattctga acaggaagat
     9721 tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc aaaaaatcat cggaatcgca
     9781 attgaacaag tagacggttt attatctgca aacggtggtt tcttctctaa tgtggcagga
     9841 aaacttgtga atacagataa tgtaacggct gggatcgaaa cagaagtcgg taaaaaacaa
     9901 gtagcagttg atatggatgt cattgtggaa tatggcaaag atatcgaaaa aatctttgaa
     9961 gaaatgcaag aagtaatcgg caaagaagta aaaaatatga cacatttaga agtgatcgaa
    10021 gtcaacgcta acgtagtgga catcaaaaca aaagaagaat ttgaaaaaga tcaagaaacg
    10081 gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg aatttgcttc gaatcaaaca
    10141 gataaagtaa aatcagcagc tggaagcggc gcacaaaaag taaaagaaaa tacagaacca
    10201 cgggttcagt aataaggaag taaaacatta ggaggaacga aacatgggat ttatttggtc
    10261 acttatcgtc ggaggaatta tcggagcaat tgcaggagca atcacgaata aaggttcttc
    10321 gatggggatt attgcaaatg tcgtagcagg tttagttgga tcagcacttg gtcaagcatt
    10381 attaggttct tggggaccaa gtattgcagg aatggcaatc gtgccatcat tgattggtgc
    10441 catcatttta gtactaatcg tatcattctt tgctcgaaaa ctgtaaaaag acttttaaaa
    10501 agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa cagccaaaca aaaatagctt
    10561 ctcatatttt tcatttggtt gggcatatta tcgaagatgc aagcttttga acactgttta
    10621 tacggaataa gaggctgtga caagactttt gtcacagcct cttatttatg cgggcaaatt
    10681 cccaattaga tttttaaaag tttttcctta aatgtgtctt tattgataca gagaagcttc
    10741 tttaataaaa caatgcctcc ctaaattttc ttgagatagg aaaggtgaac atacaaaata
    10801 atacgaccac tttgaaaacc cagatgatcc acaaatggat cgtatgatac tgatgaagta
    10861 aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac aaggaagaaa taatatctct
    10921 tttttactct tgtctgtttt agccaataat agctcgtaaa gtaatctaga caaacaaata
    10981 gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg ttgctctgct agacgattga
    11041 cgagcaggaa aaatgttggt aatggaatat agatcacgag taatgaaaga aaaaagtaaa
    11101 caataaatgg catactattc ctcctaattg tgtgagttcg ccctcatttt aaaataacga
    11161 tatttgtttg tgaagctttt tcgtgtc
//
LOCUS       DAZKMK010000092        10826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000092 DAZKMK010000000
VERSION     DAZKMK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.094"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..639
                     /locus_tag="ACG8V1_002334"
     CDS             61..639
                     /locus_tag="ACG8V1_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0330380.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            639..1568
                     /locus_tag="ACG8V1_002335"
     CDS             639..1568
                     /locus_tag="ACG8V1_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330381.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1565..3301
                     /locus_tag="ACG8V1_002336"
     CDS             1565..3301
                     /locus_tag="ACG8V1_002336"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0330382.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3364..4200)
                     /gene="pheA"
                     /locus_tag="ACG8V1_002337"
     CDS             complement(3364..4200)
                     /gene="pheA"
                     /locus_tag="ACG8V1_002337"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0330383.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4197..4706)
                     /locus_tag="ACG8V1_002338"
     CDS             complement(4197..4706)
                     /locus_tag="ACG8V1_002338"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0330384.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4699..5994)
                     /gene="aroA"
                     /locus_tag="ACG8V1_002339"
     CDS             complement(4699..5994)
                     /gene="aroA"
                     /locus_tag="ACG8V1_002339"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0330385.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6007..7098)
                     /locus_tag="ACG8V1_002340"
     CDS             complement(6007..7098)
                     /locus_tag="ACG8V1_002340"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0330386.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7115..8281)
                     /gene="aroC"
                     /locus_tag="ACG8V1_002341"
     CDS             complement(7115..8281)
                     /gene="aroC"
                     /locus_tag="ACG8V1_002341"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0330387.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8283..9347)
                     /gene="aroB"
                     /locus_tag="ACG8V1_002342"
     CDS             complement(8283..9347)
                     /gene="aroB"
                     /locus_tag="ACG8V1_002342"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0330388.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9380..10402)
                     /gene="aroF"
                     /locus_tag="ACG8V1_002343"
     CDS             complement(9380..10402)
                     /gene="aroF"
                     /locus_tag="ACG8V1_002343"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0330389.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         3001 a         2391 c         1993 g         3441 t
ORIGIN      
        1 attttctcct agaaataagt atttttaaat tacaagacta ttcttaaggc gggtaaaaaa
       61 atgccaatac gcgtaattct tatcgatgat gaacagacga ttttagatgg aatgaaacag
      121 ttgatcaatt ggtctgagca cgggtttcaa ttaataggga ctttttccag tccgctgaaa
      181 gcgcttgatt tttctttggt acatgcaatc gatatcgtgg tcacagatgt aatgatgcct
      241 gaattaaatg gaatcgaatt atcgcggcta ttaaaacagc aacaacctga aacacagatc
      301 cttatcctct ctagctatga tgaatttgat atggttaaag attcttttaa agaaggcgtc
      361 gctgattatc ttttaaagcc tcgattgagt cctgattttt ttctaaattc cttaactgcc
      421 gtttcacaaa aaatcaaaaa aaagcccaac aaaagcccaa tcaccaatcg ttatgagcag
      481 atctgcgaga atctttcaca gcagattgct ggacaaactc gtccattgct tgaccctctt
      541 ctttttgaac acaccttgtt tttctgttat ataccgaaaa acggccattc agttcaaaac
      601 aaggctctaa acaaaaacag tcgatcaccc attcattgat ggatagcgac gattcgttta
      661 ctatttatcc tgtttctacc ttttcggaag aatccggcta tgtaatcaat actgattcca
      721 aagatacact taggaagttt gttcaaaagt ttcgtcaaga agtttttcca ggtgattttt
      781 ttgttgtttc tgattctttt gcaatccaag atatcaatga aatattccaa gagatcagaa
      841 attgcaccaa aggacaacgt ttctatcata agggaaagaa ccttgcttat cagcatgaac
      901 ttcttccgtt attaaccaac cacgagcagc aagcaagtag ttatttaaca aaaatcgttc
      961 ataaagacta cgctttagct gtcaaagaac ttgagaattt cttcacgacg atattggaac
     1021 agcctattga tccagcttat ttgaaacatg aggcaatcaa tcgtttctat gcattactca
     1081 acgctttggc tgacgagcat gttgggaatg aagaactttc tttgctcaag atcgcccttc
     1141 cttctttatt agccgatgct gagtatttcg aagatttttc tattcttgtc aaagaaacac
     1201 ttgagaaact cacccacttt tctgcaaatg caaaaaaaga caatttcgat atcttgactt
     1261 tgatctacga gttcgtccag gaaaattatg agaaagaaat caatttacaa cttttatccg
     1321 aaaaatatca tttttcatat agctatctct cagccatctt tactgaaaaa tttggtatca
     1381 acttttcaaa atatctaaaa aaagtccgta tcagtaaagc caagctcttt ttgacggaaa
     1441 cacggcttac tttgacggaa atctgtgaga aagtcggcta taccgaactg ggttatttct
     1501 ccagagtttt taaagaagag acaggaatga caccttctca ataccgtaaa ggaacttttg
     1561 taaaatgaga aaaaaaatca aagagcatga actctttgcc aaacttctgt tcattttgtt
     1621 catcggtctt tttctgcaag ccatcgtcat ttctttgttt atttatcatc gatcaaggga
     1681 tgcctatatc cagctgttca atcaatcgaa tgatgtggta ctaaaaaaga ttcaatctga
     1741 atttgaaagt ttgaatgata caatcgagaa tacattagcc gcttttgatt ctaatccagc
     1801 tgtcaaaagc tatttttcca atgatccagc ccaacatatg gaacagcttc agcaactaag
     1861 aacaatccaa aaaatgaatg actctctttc aaaaatccat ccaatgatcg attacgatgt
     1921 attgatcttt ggagaaaatg gaagaacttt tgtcggaaac gacatgctca ctgccgtatc
     1981 tgccgattct ttttttcaat ctgcgattgc tcagagagtg aatgaacgtg ctgctgatac
     2041 acaaatgctt tttgcacacc acggattgac tcttcgcgat aagaaagcac ccagcgtctt
     2101 ctttgtcaaa aaactgaaaa atacattgaa tcacgtttac ggatatgctg ttttatcgat
     2161 ctcatccaag caactagcga atctttttca atctgtagta aatcctgaga tatcgaagat
     2221 ctccttcatg aacgaagaac agacgatcat cgcatcaaat gaagaaaata caattgggag
     2281 ccgatcaatg attttcaaaa aactaacagt aggtgaaaca acgatttcaa atggacagca
     2341 gttgacccgt cttcctctgt accgccagga ttgtacatta ctcagtcaag tcgatcttca
     2401 ttctttatct aaccaaatgg gcgtgatttt tccgattatt ttgtataata tcgtgacact
     2461 tgcttttgct ggctcattgg tatttatgta tttggatcga cacacaaaat cgatctaccg
     2521 cttgattgat tcgctaaaac ggctagagga agatggctta aaagctactg ttcctgtaca
     2581 aggaagccat gaagtccaat tgctagggaa tacgatcaat caattgctaa gtaatgctca
     2641 gcatcattat gaacagacga tccaagacga aaagaagaaa cgttcgcttg aaatccaaac
     2701 tatgcaagca cagatacagc cccattttat ttataatacg ctgactagct tgaaattttt
     2761 gatttggcag aaaaaaacag atgaagcagt tcaaggaatc gacagtttca ttactttact
     2821 aagaagtacg attgggaaaa aagaagaagt gattccagtc aaggaagaaa tcaaaagcgt
     2881 acaaagttac atcgatcttt tgagtatgcg atatggcgaa agaatctcgg ctaaaataat
     2941 gattcctgat gaactgcagt cattatctat gccgaatatg atccttcaac caattatcga
     3001 aaacgcctat cttcatgctt ttcacaataa aacgcaagga ttcatcacgg tgtacggcaa
     3061 aacaaaaaat cagcttcttt tattcgaagt gattgataac ggcgatggct tcgatactga
     3121 aaataccaaa caaaaaaatg atcatttttc aggaatcggc atcagccatg tagatgagcg
     3181 gatacgccta atgtatggcg atgcatatgg cttaacgatc caatcagtga ttgggacagg
     3241 aacgactgta acgatttgtc taccgcttct tcctaaaaat caatcagaat atcaaaaata
     3301 aagaatagcc aagcgctgat ttttttattt taagttaaat aaatcaatca gtattcttga
     3361 ctttcatcta cgcgtctctt ctactgtcag cactggataa cagcccaata tttgactttc
     3421 tcctccgagc atcttgattt cttggagcgc attctctact aatgtcattg gacggtccaa
     3481 caacaaatcg atcacgaaaa aatattctcc caagctagtt ttcaatggtc gggattcaat
     3541 cttacttaag ttgatttccc gccagccaaa agctgccagc attttatgaa gcatccctgg
     3601 ccgattagca ggtagtgtca agatgacact catttttact ggagctgact gctggctggt
     3661 catctttcgg tcaccgacga tccaaaaacg tgtctggttc aactcattat cttggatatt
     3721 ctcagctaag atctccaatc caacatgctg tgctgtttct aatgaagcaa tcgctgctgc
     3781 gtcttctttc ggatgttctg ctacatacat tgctgcagca gttgttgatt ctgttgctac
     3841 taaaggaaca ttaggataat gcgtttctaa aaactgttgt gattgtgcta gtgcttgtgg
     3901 atgtgaaagg atttttgtaa tctttgttgc tggattgcct aaaagctgct gcttgatagg
     3961 aagaacgatt tcacttttaa cttcgacttc tggatgtttg gaaagaagat cgattgtgtg
     4021 atgaacagct ccttccaatg aattctctac aggaacgaca gccagatcga cttgtttgcg
     4081 aaacaaagcg cgcaaacaaa cagggatgga agcatacgca gtcaattgtt ctgtaggaaa
     4141 tagctgacaa gccgcctgat aagtgaatga actttctggc cctagatagc tgacgttcat
     4201 atgcttttga tcctttctat aatggtatcg atgatctttt ctggtgtttg attggtcgtg
     4261 ttgattcgat gtgtcgcact ttcttcatac caagaaagtc gttgtgccaa taatgctttt
     4321 atttcttctc ttgtttttcc aagagctaaa ggtcgaatag acgtttcatc ttgttggata
     4381 cgatcaacta gacagtccgc ttctgcctct aagtaaagaa caatcggctg tgtttttaaa
     4441 aaacgccgat tttctgcact ttggacaatc cctccaccag tcgcgataat tttgtcttca
     4501 tttgacagct ttcttaaaat atctgactcc acttttcgaa agcttttttc accgtaatag
     4561 tgaaaatatt cggcaatcga caattggata tgttcctcaa tctttgtatc taaatctaca
     4621 tactcttttt gtaaacattg agctaaccct ttaccgattg ttgtttttcc agcgcccatg
     4681 aaaccaatca gcaaaatgct agccatgagc ttctcctcct gacacaagtt ctgctaaatc
     4741 gtcaaaaaat gccggataag agattgaaac agcttcagct ccttctagtt cgcaaggtcc
     4801 atcagctatc aaagcagcta cttgcagcat cataccgatt cgatgatcgc ctctgctgga
     4861 cacttttgcg ccatgcagag gtgtcggccc atggatgatc agaccgtctg ctgtcgcttt
     4921 tatctttgct cccattttct gtaattcttc tgccaccgca tcaattctgt tggtttcttt
     4981 tacttttaat tcttctgcgt ctttgatgat cgtgattcct tctgcttgag tagccactaa
     5041 agctagaatg ggcaattcat caatcagacg cggaatgatt tctccaccaa tcgttgcttt
     5101 cttcaaatga ctcgtcttga cacttaaatc agccgattgg ttctgttcat tgtgattcgt
     5161 ctgggtaatc gctgcgccca tctcctccaa cacatctaag ataccagttc ttgttggatt
     5221 gaccccgaca ttttttaaca acagttggct ttccggaaca agaagtcctg ctgctagaaa
     5281 gaaagcagct gatgaaatat cacctggaac agttatctgt tgaccagtta atttttgcgg
     5341 tccggtcact atgatcgttt tatcttccac tgtgatcctt ccgccaaatt gacgaatcat
     5401 ttcttccgta tggttacggg aacgttcttt ttcaacgatt acggatgtgc cttctgcttg
     5461 taatgccgca aacagcaaag cagatttgac ttgcgcactg gctacaggca tattgtatcg
     5521 gataggtgat aaagagcggg ttcctgtcac agtgattggt agatactggt cattttcttc
     5581 tccttgcaag tcagcgccca tctcgcgtaa aggttccatc actcgcccca ttggacgttt
     5641 agacaacgaa gcatccccga acagagtagt cgtaaaaggc tgtccagcca aaatacccat
     5701 caataaacgt atggtcgtac cggagtttcc catgtcaatt gattcggttg gttggacaaa
     5761 tgagtggcta ccgcgaccat gaatcttgat cacctcttcc tcttcttcga tctcgacacc
     5821 taattgttgg aaggcatgta aagtactcag acaatcttct gcgcgcaaaa aattttgtat
     5881 agtcgtcgtt ccttcagcaa ttgctccaaa catgatgctg cgatgggaaa ttgatttatc
     5941 tgctggtatc ctaacagtcc ctcttaatcc atgtatttgt tgtaataatt gcatttatcc
     6001 agcctcctat ttgatccagc aatgataact ggtattagac ataatgattc tttttgcttg
     6061 taacaaatcc tgttggtttt taaaactgat ttgcagtatt cctaaaatat cttccctggt
     6121 ctcttggatc ttcaaattaa tcaaagaaac tcccgctttt ccgatcagtc cagttacttc
     6181 tgcaataacg ccaggttcat ctggcacatc gataaataaa tcaaaaaagg caggaatcgc
     6241 gccttgctta tggacaggca actggtctcg actttctttt gcccgattga aaaaagaaaa
     6301 gatttccgat tggttctcag tcatgatcca ttctgctatt tgtgtcattt cttgttgcca
     6361 atctgccaat cgttccaata atatctgctt attgctcaac aaaatatctg tccacatgac
     6421 aggatctgag gaagcgatcc tagtgatatc tcgaaagcct cctgctgcca gttgtttggc
     6481 tcgcggatac tcttgattga attgttctgc ctgattgact aagccagctg ccagaatatg
     6541 tggaaaatga cttagcattc ctgttatacg gtcatgttct cttgctgtaa gagttacata
     6601 ttttgctttg gttccgcaaa atagttcttg cagtttttca acatcttttt tcgtcttttc
     6661 caaaggtgta aagatgtaat aggcattttc aaatagttta gcatctgctg cctgtatacc
     6721 tgatttatga gaaccagcca ttggatgtcc accgacaaac cgaagatttt tctgctcagc
     6781 ggatgtcacg acttgctgct tcgtactgct gacatccgta atgatgacgt gatcagacaa
     6841 gttgattctt tcaaggacat caagataagc taaggttgta cggacgggaa cggctaataa
     6901 gataacgtct gcttgttctg cacctgtttc aaaatcactt gcaacgtaat caacgatctg
     6961 ctgttctact gcttgctcac gggtttttga ctgactatcc caaccaacta ctatcagttc
     7021 tggattggtc tgacgaatac aaagtgccat agaagcacca atcagtccaa gccccacgat
     7081 aaaaacatgt tgtgccatct tatttgcccc ctttttagta atgtttcaag tagtcacggt
     7141 actccttcac tgcagctttc aactcaacaa gcgaatcact tgggaatttt gacaatagct
     7201 ctgtcgctac cacagtggct acaacatttt ccgcaacgac gctagctgca ggaactgctg
     7261 tactatccga acgttctaca cttgctttgt aaggttcttt tgtatcgata tcaacacttt
     7321 gaagtggctt gtacaatgta ggaatgggtt tcatgacccc gcgaatgaca agtggttcgc
     7381 cattggtcat tccgccttcg aagccgccta gattattggt acgtcggaaa tagcctcttt
     7441 cttcttccca taggatctca tccatcactt ctgagcctgg taatgaacca gaagtgaatc
     7501 cgaccccaaa ttctacgcct ttaaaggcat tgatactaac cacagcttgt gcgattttgg
     7561 catctagctt cgtgtcccat tgtacgtagc ttcctaatcc agctggaacg cctccaacga
     7621 gcacttctac aattcctccg atcgtatctc catttttctt tgtctgatcg atcaaatctt
     7681 ttattctttg ctcaactgct ggatcaacaa ctcgtacttc tgacgcttca gaaatttctt
     7741 tgatttcttg aatcgagata tttccaggta tttctgcttg gattccacca agacaaatca
     7801 cgtgtcctgc tacgatgatg tcaagttctt ccagaatttt cttggcaatt gcgccgattg
     7861 ctacacgcat ggtcgtctct cttgcagagg accgttcaag cacattccga agatctcgat
     7921 gatggtattt cataccgcct accaaatccg cgtgtcctgg acgcggacgt gcaactcgcc
     7981 gcagtgattg gtctttttca ggcacttctg ctacagacat gacagttgtc caatttttcc
     8041 aatctttatt ttctaccact aaagtgacag gtgaaccaat tgttttgccg tgacggatgc
     8101 cagacgtgat ccgaacacga tctttttcga tcttcatcct tcctcctctg ccgtaaccag
     8161 cttgtctgcg agcgagttcg tgattgattt cttcttctga cagaggtaat cctgctggta
     8221 gtccttcaat aatggctgtt aattctggtc catgggattc tcccgcagtc atataacgca
     8281 tcttacagtc ctcctaagta atctttgaat gtctctgtgg gaacaggaac gatttttgct
     8341 tgcccaatct tttctagcaa gatgatattc aattggcttc ctcttgcttt cttatcatgg
     8401 gttatcgcgt ggaataattc atctggatga cttagatcgt acgtaatcgg cagatggaat
     8461 ttttgaagta tcctttctaa agcagctgtc gatccagttg gagacaagcc gattttttct
     8521 gcatgagaag tgatggctac cattccaata gctactcctt ctccatgact gactacccca
     8581 tatcctgctg tattttcaat tgcatggcca attgtatgac caaaatttaa aataagtcga
     8641 tttccttggt caaagacatc ttcttccact acttttcgtt tgatttccaa cgtagcagta
     8701 atgattggaa cagcatgttc cagaagatct ttttcgtctt tcagctcgtt cagctgtttc
     8761 caaagcgttg catctgctat agctgctgat ttgatgattt cagcgattcc ttctcgtaaa
     8821 cgacgtaccg gcaaagtttt tagaacctca ggatcaatca atacaccagc tggctggcta
     8881 aatgttccga caaggttttt cgctttgttt gtattgactg ctgtcttccc gccaatacta
     8941 gaatctactt gtgcaagtag cgtagtcgga atttgaacga aatggatccc acgcatgaac
     9001 gtcgaggcca cgaatgctgc tagatcaccc accattcctc caccaagtgc aagtatccca
     9061 tcgctgcggg taaaatcatt atctgctaaa aaatcgtata actcagctgc ttgttctaaa
     9121 gatttactgg tctcacccgc cggtacgatt gaaaggactg gttcaaaatt agcttgtctc
     9181 aattggaaca tgattttttc tgcatatagt ggagcaacgt ttgtatcagt gatcacagct
     9241 actttttgag gtctccaaat cgtttgaagc catgatccag cctgttctag tgcgcctttt
     9301 tcaatatgaa tatcgtattt ttggttatca acatgtacag taagcattcg tttcacttct
     9361 ttcttttaaa aagctggaat taatcgagtg tctgaatctt ttccattgct tctttcatga
     9421 tcgcaacttc tttcatcatg cgtgagtaag tcttttcatt caacgattgg ggaccgtctg
     9481 accaagcatt tgcaggatca ggatggatct ctacgatcat gccatctgct ccgcttgcca
     9541 cacccgcacg agccattggt ggtacaagtt cccaaattcc tgtaccatga cttggatcaa
     9601 caatcacagg gaaatgagtt aattttttca tcaatgggac agcacttaaa tcaaacgtat
     9661 tacgtgtcgc tgtttcgtaa gtccgaatcc cacgttcgat aaaaatgaca ggagatttgt
     9721 cttcgactgc aatgtattct gcagcattaa gccattcatt gatcgtaccg gaaattccac
     9781 gtttcaatcc aacaggttta cctgttttac cgacagcaga tagcagttta aaattttgca
     9841 tattacgtgc gccaatctgt aggatatcgc tgtactcagc caccatatcg atatgccctt
     9901 catccatcac ttcagtaatc actttcattc caaattcatc agccgcttga cggatgtatt
     9961 tcaatccttc ttcttctaaa ccttggaaag catatggaga agtacgtggt ttgaaggcac
    10021 cacctcgcag aatttttgcg cccccggctt tggccattcg cgcacattct ctgatttggt
    10081 ctaatccttc gatagaacaa ggaccagcca tcgtcacgaa actgccgtca ccaattttta
    10141 ctccgtctac atccacgact gtattgccag gatggaattc tcgagaagtc aatttgtatg
    10201 tgtttaaaat acgcacagca ttttctactc cttcatagcg tgtaaatgct ccttcttgta
    10261 aacttcgcgt atcacctttt agtccaatca ctactcgttc tgttccttga tcgattgaga
    10321 ctttgaatcc ttctttttcg atatgatcga cgactttttt tatttgttct tccgttgctt
    10381 cttttttcat aattactatc attgtttttc ccgctttctt ttgtttactt atctatcttg
    10441 gctgattttc tctacttttt caaggtggtt ttacattaat tcctactaaa tgaccgtgtc
    10501 ctgttgttct cattttcgct ccttctttcc tacttatgac ccagataatc aacaaaaaaa
    10561 gatgtccctg taggaaattg cctctacagt gaacatcttc aacgagcctt aaaaagcgct
    10621 gcaaaaatat aaaatagcca ctatagatga atttctctac gtggctgtaa gtcgtgttta
    10681 aaaatcttta gccatcatag atacaaacct ggtttgtttg tacccacgaa gtcatgaata
    10741 caaacctatc taaaatagct aaagtaatac gtattcagtt ttgtaagttt tctagatttc
    10801 ataaaaaatc gctccaaact gatgta
//
LOCUS       DAZKMK010000093        10517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000093 DAZKMK010000000
VERSION     DAZKMK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.095"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG8V1_002344"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG8V1_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG8V1_002345"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG8V1_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG8V1_002346"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG8V1_002346"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0330390.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8V1_002347"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8V1_002347"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0330391.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG8V1_002348"
     CDS             5704..6489
                     /locus_tag="ACG8V1_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0330392.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG8V1_002349"
     CDS             6502..7353
                     /locus_tag="ACG8V1_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0330393.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG8V1_002350"
     CDS             complement(7477..7929)
                     /locus_tag="ACG8V1_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330394.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG8V1_002351"
     CDS             complement(8394..8762)
                     /locus_tag="ACG8V1_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0330395.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="ACG8V1_002352"
     CDS             8912..9394
                     /locus_tag="ACG8V1_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0330396.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="ACG8V1_002353"
     CDS             9431..10441
                     /locus_tag="ACG8V1_002353"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0330397.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DAZKMK010000094        10489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000094 DAZKMK010000000
VERSION     DAZKMK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.096"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG8V1_002354"
     CDS             34..657
                     /locus_tag="ACG8V1_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330398.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8V1_002355"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8V1_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0330399.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG8V1_002356"
     CDS             2153..3034
                     /locus_tag="ACG8V1_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0330400.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG8V1_002357"
     CDS             3037..3807
                     /locus_tag="ACG8V1_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330401.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8V1_002358"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8V1_002358"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0330402.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG8V1_002359"
     CDS             5118..6281
                     /locus_tag="ACG8V1_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0330403.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG8V1_002360"
     CDS             6398..6808
                     /locus_tag="ACG8V1_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0330404.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG8V1_002361"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG8V1_002361"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0330405.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACG8V1_002362"
     CDS             9381..9665
                     /locus_tag="ACG8V1_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330406.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACG8V1_002363"
     CDS             9825..10061
                     /locus_tag="ACG8V1_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0330407.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACG8V1_002364"
     CDS             10058..10456
                     /locus_tag="ACG8V1_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0330408.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3658 a         1764 c         2222 g         2845 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatca
//
LOCUS       DAZKMK010000095        10406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000095 DAZKMK010000000
VERSION     DAZKMK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.097"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..364)
                     /gene="acpP"
                     /locus_tag="ACG8V1_002365"
     CDS             complement(122..364)
                     /gene="acpP"
                     /locus_tag="ACG8V1_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0330409.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(486..1487)
                     /gene="plsX"
                     /locus_tag="ACG8V1_002366"
     CDS             complement(486..1487)
                     /gene="plsX"
                     /locus_tag="ACG8V1_002366"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0330410.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1536..3572)
                     /gene="recG"
                     /locus_tag="ACG8V1_002367"
     CDS             complement(1536..3572)
                     /gene="recG"
                     /locus_tag="ACG8V1_002367"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0330411.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3689..5371)
                     /locus_tag="ACG8V1_002368"
     CDS             complement(3689..5371)
                     /locus_tag="ACG8V1_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0330412.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5423..5785)
                     /locus_tag="ACG8V1_002369"
     CDS             complement(5423..5785)
                     /locus_tag="ACG8V1_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0330413.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6055..6243
                     /gene="rpmB"
                     /locus_tag="ACG8V1_002370"
     CDS             6055..6243
                     /gene="rpmB"
                     /locus_tag="ACG8V1_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0330414.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6481..9150
                     /locus_tag="ACG8V1_002371"
     CDS             6481..9150
                     /locus_tag="ACG8V1_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0330415.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9605..10351)
                     /gene="truA"
                     /locus_tag="ACG8V1_002372"
     CDS             complement(9605..10351)
                     /gene="truA"
                     /locus_tag="ACG8V1_002372"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0330416.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2842 a         2240 c         1868 g         3456 t
ORIGIN      
        1 acataatttc tgtttatcgc cagaattatc agaaaaatga cttttacgtg tcaaaaagat
       61 taaaaaaaga aggataaaaa gctgcatgca gctttttatc cttctttgct tttatttgaa
      121 attatttaag atggctgctg atataatcta ctgcgccacc gactgtttca atttgttcag
      181 cgtcttcatc tgaaatttcc gtaccgaatt catcttcaag ctctaaaacg aattccataa
      241 tactaattga atcagctttt aaatcatctt tgatgttcaa ttcatccgtc actttatcgg
      301 tatctatctc aaaatgattt gaaataattt tcgctacttt gttaaatact tcttcgcgag
      361 tcaacttttt cacctccaag aatcatacac ttgtcaatta cctacaccat ggctaatttt
      421 actggcattt ttcaggaatg tccacctttt tttcaatgga acggatcgct tcagatatcc
      481 aaatactatt cttcgctttt ttcaaattgt tcgactaatt gtccaactac atctgtctca
      541 agcattgtgt ggatttggcg gatcgtatag cgaacagcat cggggcctgt tgctccatga
      601 gttttaatga ctggtgcttt caagccaaac aaaacggcac cgccatgttt ggaataatcc
      661 atttctgctt tcaaagaacg taagccgtct tttaaaagca tagcgcccat ttttcctttg
      721 atcccttcat ttagaatagc agattttaat agattcatca tattcattgc tgttccttca
      781 atagatttca aaacagcgtt gcctgtaaat ccatctgtga ccactacatc agccacacca
      841 ttgagtagct cacgagcttc tacgtttcca ataaaattca agcttgtttc attctgaagg
      901 agttcatagg cttttttcgt cacttcacta ccttttgttg cttctgttcc gttgtttaag
      961 agtgcgacac gaggacgtcc gatacctcgg actttacgtg cataaaaaga acctaaaata
     1021 ccgtattgca acaaatgttc tggtttgttt tctgcgttag cgccaagatc cagcatatcg
     1081 aaaccggcac cttctttccc tactactggc aaggtagaca tcaagccagg gcgttcgata
     1141 tttttgatac gtcctacgat gaacaaccct gctgctagta gcgcgcctgt attccctgct
     1201 gaaaatacag catctgcttc accgtttttc actgcttgtg cagcaaggac cattgatgca
     1261 gtcttttttc gtcgaattgc ttttactggc tcatcatcac tattgatttt ttcgtctgta
     1321 tggataatcg tgatgttttt ttcatttgtt aagtatttct tgatttctgc ttcttttccg
     1381 taaagtaaga attctacttc tggaaaatct tgctgcgcta aagccacgcc ttctacaatt
     1441 gcttgaggtg cgtgatctcc gcccattgca tctacggcaa ttttcattgt ttccactcct
     1501 ttttccgtca tcagtataaa gtatagcaca gtctgctaat cgaaataacg attatcctga
     1561 ttttctattt ggaggctttg cgctaaaggc tgatactcag gaagcatctg ccaatccttt
     1621 atttgccaaa gggctgcagc ctcttctctg gctacctcca aaacatttcc atccgtcacc
     1681 aagtcagcaa tcgcaaattg aggcagacca gattgacgga agccaaatac ttcaccagga
     1741 ccacgtaatt ccagatcttt ttcgcttagt acaaaaccat tggtggtctc tgtcatgatt
     1801 ttcatccgtt ccacacctag ttcattttta ggattagcaa ctaagataca gtaggaagca
     1861 tcacttccgc gtccaacacg tccacgaagt tggtgcagct gtgccaatcc aaatcgatcc
     1921 gcatccatga tcagcatgac ggtcgcgttc ggcacgttta cccctacttc aattacagta
     1981 gtcgaaacta atatctgcat ctggttgtct ttgaaggctt ccatgattac ttctttttcc
     2041 tgattcttca ttttaccatg taacaagcca acttcatatt ttggtgcaaa tagttcacga
     2101 agtttttcat agatttccac tgcatttttt acatctaatg cttctgattc ttcaatcagc
     2161 ggacagatga catacatttg atgtcctctt gccagttctt ttttcgtcca atccagtaca
     2221 ctgtccaatt gaggtgttct tacccagcgt gtttcgatcg ggatacgtcc agctggaagt
     2281 tcatcaatga tagatacatc catctcacca tatgctgtaa tagctaatgt tctaggaatc
     2341 ggagtcgctg tcataaataa gacatctggt cgccagcctt tttcacgaag tactttccgc
     2401 tggttcacgc caaaacggtg ctgctcatcc gtaataacta gacctaaatt ctgaaattcg
     2461 actccatctt ggatcaatgc atgtgtgccg atcacgacgt taatttcccc ttgctctagt
     2521 ttttcaagga gctcacgtcg ttcttttgct ttcgttgacc ccgtaatcaa tgcgatggtt
     2581 acttcattgg gatcaaacag ctgttgcaag ctttccatat gttgttgtgc caagatttct
     2641 gtaggcacca tcaacgcacc ttggaaacct gctgtaacgg tagcgtataa cgcaatcgcg
     2701 gcaaccactg tttttccgct acctacatct ccctgcaaaa gtcgctgcat atgctgcgga
     2761 ctgcgaagat cccgacagat ttcattcgtt acacgttttt gtgcatttgt cagttcaaaa
     2821 ggtagctttt gagtaaattg tttcaatttt tggacatcat aaaggatctc caaaccattc
     2881 gtttctgctt tttctgcttt tttcaatcct tgaagacgca tttgaaataa aaagaactct
     2941 tcaaaaacga cacgtcgttt ggcttgatgg ttttcttcgt ggtcttttgg aaaatgcata
     3001 cttcttacag cagaagctcg gtcaagcagt cgatatttct ccacaaggtt gtttggcaga
     3061 ttttcttcaa tcaatgtgcc gtattcttga aacccttttt tgattagatc aactaatgtc
     3121 gtttgacgaa ttgatttgtt gacgtgatat atcggcgaaa agtcatccat tgtttgagaa
     3181 cctaaaatct tcatcccgtt caaggcttta cgctttgcat cccatttacc gtatacggcg
     3241 atttcctccg ataaaatgac tttttctttt agataaggtt gattaaaaaa agaaacgttg
     3301 aacacatcat gttcttgcat catacgaaat tgcaatcggc tttttttata accaaatcga
     3361 ttcatcacag gaggcgagac tacgattcct ttcaaagtaa ccttttcttg atcctggatc
     3421 tcgtttagat ttctttcctg aatatcttcg tagcgaaaag gataataagt cagtaagtct
     3481 tcaatcgttt ggatgcccaa gctggcaaga ctagctgctc ttttttctcc gactcctgcc
     3541 agcatagtga ttggatcatc taaagaacgc ataaattttt cctccctcct ttaacaaaaa
     3601 aaggacttgg tcagcgatca tacctgatca ctaaccgaag gccttttatg aatgaatact
     3661 gtgttagaaa gatattctga cacagaaatt attcagcaga aaatagataa ggatagacag
     3721 gctgatctcc ttggtggatc tcgatttcca aatcaggatg tttttctgtt agtttctcag
     3781 caaattcatt tgcttgttca actgttcctt catcacctgc caagatcgtt acgatctctg
     3841 tgtcctcatt gatcatttgt tctagtgtac ttaacgctgt tgctgtgata tctgggttag
     3901 agatcaggat ttttccatcg atcatgccca aaaagtctcc ttctttgatc tctacaccgt
     3961 caattgaggt atctctgatc gcgtgagtga cttgaccgct cacaacgcta gacaacattt
     4021 ctgtcatcgt ttctttgttt tcttctaaag aagcttgatc attgaatgaa agcatagctg
     4081 tcaatccttg ggaaatcgtt cttgaaggaa caactgctac tgggatatct gctacttctg
     4141 ctgcttgatc agctgccatg aagatgttct tattgtttgg caagacaatc actttatctg
     4201 cgtttacttc ttgaatcgct ttaacaatgt cttccgtact tggattcatt gtttgtccac
     4261 cactgataac ataattagcg cctaaacttt caaacagctc ttggacgcct ttaccagcag
     4321 cgatcgctac gataccatac gggatacgtg gtatttcttt tttcgcaaac tcgttcactt
     4381 cttcatcgtg ttccaaaatc gtctcatgct gcaagcgcat gttgtcgact ttgattttga
     4441 ctaatgaacc gaatttttgt ccataattca agacttctcc tgggttttct gtatgcacgt
     4501 ggactttgat gatctcgtca tcattgacca caagtaaaga atctcctata ccatctaaat
     4561 agttacggaa ttcttcgtaa tcgaatgtac tatcgactgt aggtccttct ccgattttta
     4621 ccatgatttc tgtacagtaa ccaaaatgga tatcttctgt cgccatttga ccttggacac
     4681 tgcgatgatg ttctgcattg accatatcat ccatttctgc tggagaaggt tcatatgttt
     4741 catccgcttg gaattctcca ttcaatgcac ttaaaaagcc ctcataaaca aacagcagtc
     4801 cttgccctcc actgtctacg acaccgactt cttttaggac tggaagcaag tctggagttt
     4861 tagctaaagc acgctttgct ccttttacaa cagctgtcat tacttcaacg acatcatcag
     4921 tactttttgc tttcttttct ccagctttcg ctgcttctct agctactgtc aaaatcgttc
     4981 cttctactgg tttcattact gctttatacg cagtttcaac tccatgagtg aaggctgcag
     5041 caagatcaga agcatttaat gagacaacat ctggaatttg cttagaaaat ccacgaaaca
     5101 attgagacaa aataacgccg gaatttccgc gcgcacccat cagcaatcct tttgaaagca
     5161 ttgctgctaa ttctcctact ttttctgagg ttgaatctac tactgcctta gctccactag
     5221 tcatagatag attcatattt gttcccgtat cgccatctgg cacagggaat acatttaatg
     5281 aattgacgta ttctgcattt acttgcagac gattcgcacc tgcctggacc atttcctgga
     5341 actggcttgc acttatttct gttacactca cctagataat cctccttcag atcgactgct
     5401 gagttaccag ccagtctcct gcctagtctg gcagtacacg aacaccttgt acaaagacat
     5461 taactgaatt ggcagttacg cctaataatg cctctagatt gtatttcact ttttcttgga
     5521 cgttgcggga aacttcagag attttcgtac catagctgat aatcgtataa acatctacag
     5581 caataccatt atcttcttgg cggacaacta ctcctcttga gtaattttct ttacgcaaaa
     5641 tctcagtaat attatcttta atttgatttt tgctggccat tcctacaaca ccgaacacat
     5701 ctgtcgcggc gccaccaaca actgttgcaa ttacatcatt ggtaatttca atggttccag
     5761 ctgacgtttg aatttttaca gccatgaatg agcctcctta acgggtaaaa aactcccatt
     5821 tttcttctat catattttat catagcgcaa cttgaaataa aaggcagtta cccaaagtac
     5881 tcaaaaacct tgcaaaattt caggtataac cttgtttaat ttacaattat tcttctcaat
     5941 tgtcaattgt atctatgaaa atgtacttgc aaattcccat tctatatgat atgttagtct
     6001 ggtatgagct gaattattct attgctccaa atcaaggcaa aggaggaaat taccatggca
     6061 aaagtttgtt actttacagg tcgcaagaca aaaagtggaa acaaccgttc acacgctatg
     6121 aactcaacaa aacgtactgt taaacctaac ttacaaaaag ttcgcgttat ggtcgacgga
     6181 aaacctaaaa aagtttgggt gtcaactcgt gctttgaaat caggaaaagt tgaacgcgtt
     6241 taattgaatg atacttggac cgttcagata gtgaacggtt ttttatttaa taaaaatttt
     6301 taagggtggg acttttgatt cctacccttt ttttgcgttt atttttgtta tacgaacaaa
     6361 ttatgaagtt atttattcta aattttattt ttttgtgaaa actagactct ttttaagcag
     6421 agaaaaacaa ggatatgata aactgaatac aacttcatag aaatttggtg gaaaataaat
     6481 atgaaaaaaa atttaaagtc attctttaga gaaaacggac gctacgcagc agctagtttc
     6541 ctgattccgt ttttgatttt agcacttgtt tatctaagta tcggaatcta tcctggaagc
     6601 agcagaagta ttttggcaag tgatgccttt tcacagtttt ctaattttca tgccagcttc
     6661 cgaaatgttc ttcttggaaa gcaaagtatt ttttatacat ggaatgcttc tctaggattg
     6721 aattatttat ctttaatttc ttactatcta ggcgggatct tcacgccact ggtgttcttt
     6781 tttccaaacc agatgatgcc agatgcactg tatgttctga ctttgcttaa ggtcggctgt
     6841 gccggattgg ctttttggtt tcttgcacgt acgttcaaga ttcctcgctg ggggcatgtt
     6901 gcacttagcg tcagttacgc attgatctca tttatcactg cacattcgga gatcatcatg
     6961 tggctggatg cattttttta tcttccatta gtgattttgg ggattcatcg tgttatggat
     7021 cttaaacggc cgaaactttt atttatcagt tatttactct tgtttttatc cagtttttat
     7081 atgggtttta tgattggcgt attttctttc ttatatttta tcgcccgtct attgacaaac
     7141 tggtcagaat ataaaaaaac gattgttcct tatggtatca cttctttact ggcaggcggc
     7201 gcttcaatga tcattgtatt gccagcagtg ctggatcttc ggtcaaatgg cgaggcactg
     7261 agtcagatca ctacgttgaa aacagaagcg acttcttatc tggatctaat catgaaaaat
     7321 atgattggtg tttatgacac gacaaaatac ggatcgattc cgttcgttta cgcaggtctg
     7381 ctaccattag tcctctgtct gttttatttt gccagtcgga aagtaccgtt gaaaaataaa
     7441 tttttgtacg gcagtctttt cgccatactt atcgcaagtt tttatcttgt tccattgaat
     7501 ttgttctggc atggtatgca tgcaccgaat atgtttttat tccgttatgc ctatctgttc
     7561 tctttcttag tgctcctgct tgcaggaaaa ggatgggaaa agatagaaca agacgatcgt
     7621 gggctgttgt taggaatcat tttggtcgta gctgctctct ttgcggtggc atggggcatc
     7681 aaacctgaag gaagctacac ctatgtgacg atgacttcct ttgtattgac agtggttttc
     7741 ttagggattt atgcttttgc tgtgggattt tatcagcata aaaaactttc attcaatcat
     7801 ctgagtatat tgttattatt gatcatgtct gctgaagcac tggtcaatac gaacagtatg
     7861 atccatggca ttcttgatga ttggaattat gcttcccgca gtttatatac tgatccttat
     7921 cctgacttga aagctcttgt aaatgaaaca aaaaaagatt ccgactcgtt ttaccgcttg
     7981 gaaaatctgg atccagtatc tgcaaacgat tcgatcaact atggttacag tggtatcagt
     8041 ttgttttctt ctataagaaa tcgtaattct tcctcttacc tggataaatt aggtttccgt
     8101 tcaagaggga caaatttgaa tatccgttat aacaataata ctttgctgat ggatgggttt
     8161 acaggaatca aatataacat ttctaaaaac gaaagcacgt tcagtaaata tggcttccgc
     8221 caagctggtc aaagcggcga ctatacactg tatcagaatg tcaatgcatt gccactagca
     8281 tttactgcac cgttgtccgt taatgatgtt gagcagccag tgacagataa tttgaccagt
     8341 cagacaaact tgatcaatac attgtctggt ctaaatgaac agtattttac attctataca
     8401 cctactttga agaatcagtt gaatacgacg atcacaaaga caacgactgg cgtcacttac
     8461 tcagaaacag cagataacca gccaaaagag ctcacttggg aagtccatgt gcctgcaaat
     8521 acacaagctt acttaagttt gttcccaacc aattttgcgc aattagaaag ttctagtgcg
     8581 acgatcactg tcaatggtac atcgcgaaaa acgcaaatca acatttctgg tcaatactat
     8641 gatcttggct attatcctca agagacaaca gtgacattta ctgcaagttt ttatgggacg
     8701 aaagaaataa gtttcatgga gcctaaagtc gttggtctag atgttgtagc ctatcaagcc
     8761 gcaatgaatc agatcaaaga aaaaggtgtc gaaatggaag ccactggcag aaaagcttct
     8821 gggactgtaa ataccgctga agattccatg ttggtgacaa ctattcctta tgacaaagga
     8881 tggaaagcta aaattgatgg gaaaccagtc aaggtcagca atttcaaaga cgcattcttg
     8941 atgataaaag ttccagcagg taaacatact gtcactttca gttacttgcc tgaaggattt
     9001 acgataggtg caatcctttt tgtcctatgt attgcattgt ttattcttta tgtctggtac
     9061 cttgaccgca atcgcccctt ttatggcacg attgatccag caatagaaga acaaccacga
     9121 aacacacaca gaagaagacg tagaaaataa agaaacgatg gaacctgttc agactgtccg
     9181 caccaagggc aatcaaaaca acgttccatc gttttttggt tacagaaaaa ataggtaaca
     9241 aaaagatgat tcacttatca acgcctcgtc cacaagtcac gatattcatc aaagatgaga
     9301 agctgttttt tgaaattgct ctttgtgact cagagttaaa tgacaggctc acgacctctt
     9361 tcttaaacgg tgttcaatca gctgaccttc ggtattagct caacaaacgg caaaatatga
     9421 ggagctattt tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacgacctc
     9481 ttccttaaac ggtgttccag aagtagcttc ttcgaaaata agccgaaatt ttgcaaaatt
     9541 ttgagaagta attttcgtaa ataccttctt atttcttgaa gctgaccact tctatcataa
     9601 cctcttattc atatttcact tcatacaaat aaagtccttc agggtgtgct gtaggtcctg
     9661 ctgcattacg atcttttttc gcaataatat ctgggatact atcttcaggc aatcggccat
     9721 tgccaatttt caaaagcgtt cctactaaga tccgtatcat cttatacaag aagccatttc
     9781 ccctaaatgt aaacaacaat tcgtcttttg tttcatttac ctccatctta gcctcgtaga
     9841 tcgtacgtat tttatcctct atcgagcttc ctgatgcaca aaaagaggtg aaatcgtgag
     9901 tccctaataa atcaggaagc gcccgctgaa ttttttccaa atcaagtggg taaggaaaat
     9961 aacttgcata atggcgtcga aaaggactgc gtggtttccc tatatccact cgaaattgat
    10021 acgtcttttc tttgaccaaa tatcttgcgt ggaagtcttc tgaaacaatt tctacttgtc
    10081 ttacagcaat atcctctggt gactgggtat ctagcgcgaa acgcattcgt tctaacggac
    10141 gctcctctgg ataatcaaaa tggattactt gcccgatcgc atgtactccg gcatctgtcc
    10201 ttcccgaacc aaagaccgtg atttcttttc catttgccat tttttgtaat gttttttcta
    10261 cttcttcttg aacagtacgc ccatttggct gtttttggaa tccattaaaa tttgttccgt
    10321 cgtacgcaat gattgctttg taacgtacca tgtttttctc tcctttgttc ctaaaaggtt
    10381 atctggaata atgatttttc tataaa
//
LOCUS       DAZKMK010000096        10139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000096 DAZKMK010000000
VERSION     DAZKMK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.098"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..133
                     /locus_tag="ACG8V1_002373"
     CDS             <1..133
                     /locus_tag="ACG8V1_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0330417.1"
                     /translation="NPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTFIYNEF"
     gene            144..1658
                     /gene="dltA"
                     /locus_tag="ACG8V1_002374"
     CDS             144..1658
                     /gene="dltA"
                     /locus_tag="ACG8V1_002374"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0330418.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1655..2860
                     /gene="dltB"
                     /locus_tag="ACG8V1_002375"
     CDS             1655..2860
                     /gene="dltB"
                     /locus_tag="ACG8V1_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0330419.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            2905..3138
                     /gene="dltC"
                     /locus_tag="ACG8V1_002376"
     CDS             2905..3138
                     /gene="dltC"
                     /locus_tag="ACG8V1_002376"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0330420.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3141..4409
                     /gene="dltD"
                     /locus_tag="ACG8V1_002377"
     CDS             3141..4409
                     /gene="dltD"
                     /locus_tag="ACG8V1_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0330421.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4550..5632
                     /locus_tag="ACG8V1_002378"
     CDS             4550..5632
                     /locus_tag="ACG8V1_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0330422.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5632..6435
                     /locus_tag="ACG8V1_002379"
     CDS             5632..6435
                     /locus_tag="ACG8V1_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0330423.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6505..7245
                     /locus_tag="ACG8V1_002380"
     CDS             6505..7245
                     /locus_tag="ACG8V1_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0330424.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7256..8260
                     /locus_tag="ACG8V1_002381"
     CDS             7256..8260
                     /locus_tag="ACG8V1_002381"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0330425.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8305..>10139)
                     /locus_tag="ACG8V1_002382"
     CDS             complement(8305..>10139)
                     /locus_tag="ACG8V1_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330426.1"
                     /translation="NNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSN
                     QMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGN
                     HQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLP
                     PKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLS
                     CFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGI
                     FCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVA
                     CTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDR
                     RQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEK
                     ILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQL
                     LIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELD
                     DQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3253 a         1683 c         2049 g         3154 t
ORIGIN      
        1 taatccaaaa ttgaattatt ggggaaaatt catcattcga acaatttttt atagtgcagt
       61 actgctgttc ctgatttatc tgtatcacta taaaaatatt caaggaggaa cttttatcta
      121 taacgaattt taggaggaag agtatggaaa tcaaaacgat tattgaagca atcgatgatt
      181 ggggaataaa tgaacctgat cgagttgctt atcaggcgga tgagacgcat acttttggtg
      241 aattgaaaca agcatcagac gcacttgctt attatttaga aaaaaaggta gaaggcgatg
      301 gaccaattgt cgtttttggc aatttggagt ttgaaatgat tgtctcattt ctaggggttg
      361 tcaaatctgg ccatgcctat ttgccgattg aagagcatac acccaaggag cgtatcctat
      421 cgatctttcg ggtggctaaa ccatctatgg tcatcagtat cggggactgg atagaagata
      481 ttccttcggt tcctgtcata acaaaaaaag aatttcaaga aatcaagaag ataccagtag
      541 gattttatcc cagaaatagc gtaaaaggta cagaaaattt ttatattatt tttacttctg
      601 gtactactgg agaaccaaaa ggcgtacaaa tcagtcatga taatctagtc agctttgttc
      661 aatggacgat ggaagacttt gggatcaagc ctggtatgca tttcttagcc caagcgccat
      721 tttcttttga tttatcggta ttttctattt atcctgcatt agtttctggc ggaatgctga
      781 agccgttaaa taaagcagtt gttcaagact ttcgtcaatt attcgcgaca ttgccagcct
      841 tgaagttgaa tgtgtgggtt tctactccat catttatgga tatttgcttg atggaaccta
      901 cattcaatgg agaaaatgtg ccggaattgg acatgttttt attttgcgga gaagaattga
      961 cgaaaaaaac tgcagaaagt ctacttgatc gattcccaaa tgcacggata tataatactt
     1021 acggtccaac agaagcaact gtagctattt caagtattca gattgaccag catgtactag
     1081 atagttatga ccgactaccg attggctacg taaaaaaaga cacgaaagtt tctattgtcc
     1141 aagaaggtaa acctgtttca aaaggagaaa cgggtgaaat cattattgca ggaccaagcg
     1201 tatcaaaagg ttacctgaac aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg
     1261 acttagcaag ttaccataca ggggatgcag gtagactaga tgaatatggg cttttatttt
     1321 atgaaggacg tatggatcag caggtcaaat tacatggata ccggattgaa ctcggggata
     1381 tcgaacatta tctgctccaa gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc
     1441 aaggaacaaa agtacagcag ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc
     1501 cggattttca gttgactaaa tctatcaaag aacaattact agaagttgta atggattata
     1561 tgattcctca gaagttccat tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg
     1621 atcgaaaaaa attaacagct gaggtgaatc ctgaatgatg ttacctcata tgatccctta
     1681 tgcagagccg ttttattttt ttcttttagc cattgcgctt gtaccgatcg ttctatcttt
     1741 attgatatgg gaaaaaagat taccagttta tcaatcagca gtgacacttt tctttttatt
     1801 tgtcagcttt ggaggtaatt attggcaaca gggagcggcg ctgattgggt atgttttgtg
     1861 gcagacgata cttgtatggg gctattttgt ttatcggcaa aggaaaaatc agacaggatg
     1921 gttttttgcc gccgttattt tagcgataat tccactgttt ttaacgaaag tgactccttt
     1981 ttttgaacat ggaaaagcat cgttacttgg ttttttagga atctcttatt taacctttaa
     2041 atctgtacaa atgatcatgg aaataagaga tggcatgatc aaaacatata agccatttcg
     2101 ttatatccag tttctgctgt tctttccaac aatctcttct ggaccaatcg atcgctatag
     2161 gagatttgaa aaagatcagc tccatccgcc tgttcgtgat aaatatgttg atcttttggg
     2221 aaaaggcgtc caaaatattt ttgtcggttt tttatataaa ttcattatcg gttattattt
     2281 tggtcaagta ctgcttcctg tagtgggacg cattgcctta cagcatggtg gtatttcatg
     2341 ggcattagta ggttatatgt acgtgtacag tatgtatttg ttttttgatt ttgctggcta
     2401 tagcttattt gcagtcggta caagctacat gatgggatat cagacaccaa tcaatttcaa
     2461 taaacctttt ttatcatgga atatcaaaga attctggaat cgttggcata tgacgctgtc
     2521 cttttggttc cgtgattatg tatatatgcg attgatgttc ttcatgatga agaaaaaatt
     2581 ttttaaaagc aagatcgtaa tgtcaaatat cggttatttt gctttgtttc ttctaatggg
     2641 tatatggcat ggattgacgt ggtactacat cgtatatgga ctgtatcatg cttgtctgat
     2701 ctgtctgaca gatgcatgga tccgatttaa gaagaagcat aaagagcagt tgccttctaa
     2761 caaatttacg catggcttag ccgtattttt gacatttcaa gcagtatgtt ttagcttcct
     2821 gattttttca ggatttttag ataaactttt ttttagctaa cgaatagtga catattaatt
     2881 tatataacaa aggagaaaag gaaaatggaa gaacaaattt tatcaatctt agaagaaata
     2941 acaggaacag atgaagtaag aaaaaatcgt gatattgatc tatttgatga agggttgctt
     3001 gattctttag catctgttca attattagtc gagctagacg gagagctgga tatccaagtc
     3061 cctgtatctg aattcgagcg agaggattgg tgcacaccga ataaaatcat tcaacaagca
     3121 actgcattga agggttgagt atgaaaaaga aactttttgc tattttcggc ccggttgttt
     3181 tagctgttgg cttactgttt gtgtttttta cctcctccta tcaagtagat atctctaatc
     3241 cagaaacgat tagacgtgcc tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga
     3301 taaaaaatta tgcattatct gaaaaacaat atattccttt ttttggctca agtgaattaa
     3361 gcagaatcag tccatttcat ccgtctgttc tggcagaaaa ataccagcga aactatcgtc
     3421 catttttgct gggagcaccc gggacacaat cgttaagcca atatatgatg atgcgttctg
     3481 caggagatgc tatgaaaaac aagaaagtcg tctttattat ttctcctcag tggttcgtta
     3541 aaaatggtgt aaaaactgat tactttaata cgtattattc tgaacttcaa acatacgact
     3601 ggttgttttc aatgaagaaa gtaacgcctg ctgatcgcta tttagctaga agattattaa
     3661 ctttctcaaa agtaaaagag aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag
     3721 ggaagctacc tttacctgaa tctttgaatc agctgcgcag ccaatggaac atgctgaaga
     3781 gagaggatga agtattcagc aatattgggt tgaaagatcg acagcaaaaa atcgatcatg
     3841 agtcaaaacg attaccaaaa caataccaag aaacagaatt atccatttta gcaaatcaaa
     3901 ttggagaacg agaaacgacg aataatccat tcgggttgaa aaacgatttt tatacgcatc
     3961 gtatccgagc ccacgagcca gagctcaaac agtcacaaaa aaattgggat tatcgttttt
     4021 cgccagaatt ttctgatttt cagctggtct tggatcaatt agcaaaaaac cataacgaag
     4081 tattgttcat catcccaccg gttaacgaga aatggagcga ttataccgga ctatctcaag
     4141 aaatgttaca aggatttgcc aaaaagatca aatttcaatt aaatagccaa ggattcaatc
     4201 gaatagctga ttttgtcaat caagcgggta ccaactattt tatggaagac acgatccatc
     4261 taggctggaa aggctggttg gctgcagatc aacagatcag accgttttta gaagaaaatc
     4321 atattactgc tagtaagtat catcttgacg atgctttttt tagcaaatct tggcaacatc
     4381 aaataccaga taaactacaa ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt
     4441 ctttattttt gaatattctg aataaaagat aaaaatacca agtttgcctg aacaaaaatc
     4501 ataagcaatg gtataatcac aatatgaata tagaaatggg ggaaaacatt tggatacaaa
     4561 agaagaacag ctacttattg atttaacttc tgcaaaagga gtacctggaa atgaagaaga
     4621 ggtaagagaa gtattcagag aatatgccaa accattcgct gatgatattt tttatgacgg
     4681 tttaggcagt gtgattgcca aacatggtgc tggcggaggc cccaaggtct ttatttctgg
     4741 acatatggac gaagttggtt ttatggtaac aaaaataacc gaaaaaggat tcttggaatt
     4801 ccaaactctt ggaggttggt gggggcaagt catgcttgct cagcaagttg aaatcaaaac
     4861 acgtgaaggg aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg ttttaacacc
     4921 tcaagtaaga aataaacctt atgaaataaa ggatatgttc attgatattg gcgcatcaag
     4981 ccaagaagaa gccaaagagt ggggcatacg tcctggagat atggtcactc catatattga
     5041 atacaaacga atgaatggca gtaaatattt actggcaaaa gcatgggata atcgtatcgg
     5101 gacagctgtt tctctgcgag tattagagaa tctatctaaa gaagcacacc caaatgtttt
     5161 attcgcagga agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa caagtacaca
     5221 ccttgtaaat cccgatattg cttttgcttt agatactggt acagcaggag acactccagg
     5281 aatgacacca aaagaagcag attcgatttt aggaaaaggc ccacagattt tgatttttga
     5341 tgcatctatg atcccgcata aaaaattatt gaattttgtt attgatgtag cagaggaaat
     5401 ggatattcca ttccaatata cagtgatttc tggcggagga actgatgcag gacaaatgca
     5461 tttgacaaga gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt atctgcattc
     5521 ccatacatca gtgattcatg aagatgatta tttgaataca gtgaaactag tcacagaatt
     5581 agtgaaacgg cttgatagtg aaacagttga aaaaattcgg agttatgaat aatgaataat
     5641 agaataccta tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga
     5701 atcaatactt ggcttgtttt cttatctata atatttacga gcgtcatcca gaactttttt
     5761 gacggtttca gtccttttcc ggaagaaagc atgcagcagc acttattgag ttttctagtc
     5821 gataatttac ttttattcgc attcaactat gcattgtttt acattgcgct agtagttgta
     5881 cggggtggca gaccaaaaag taacctttta ttttcagttt tccaaagaaa gtattttggt
     5941 tcgatacttg gcatcaattt gatcaattca gtcatcaact ggctactcaa cagtttaatc
     6001 tttctgccca tgtttttaat cagcggagtg agtacttata tgcagctgct tttcagttca
     6061 aatgcggtac ctacgggtgt catgccagaa gattttcttg atatcagctt tgttttatcg
     6121 atgataggga tgatttttgt ttcagcactc atcatgagta ttgttggcgg tttattccaa
     6181 tttgctgcat ggacaaagtt cgactatcct gatttgtcgg ttattcaatg tttgaaatat
     6241 ggctggtttt tattgaaaga tagaattggt acgtatattt tgttgcagct gtcttttatc
     6301 ggctggtatt tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt cattgtttat
     6361 gtgaatgtgt cagttgcaga attttatgaa caagcacgga ttgaaaaagg aggtccagac
     6421 gcctatttta cttagcatgt aagcagttta ttcatcaaaa aaataacttt tgtggtagga
     6481 taaatctttg agggggaatg tacgatgaaa ttaactgttt taggatgctt aggagcgtat
     6541 ccttataaaa atcaagggac aacaggttat ttgcttcaat ctgacgggtt caatttgttg
     6601 atcgatgcag gtagtgctac gctgatcaaa ttacaagaac accttgatcc acttgatcta
     6661 gatgctgtga tccttagcca ttatcatcat gatcatattg ctgaccttgg tgtattacag
     6721 tattactggc aacttcatcc taagagaaat ccggagacat tgctttcaat ttatggacac
     6781 acaaaagatt ccattcactt tgaagaattg acgttggaaa atgtctcaaa aggacatcct
     6841 tattttgaag ctgaagaatt aaaaattggt ccttttctga ttacttttat ggaaacaatc
     6901 catccagtag tatgttacgc catgcgtatc gtagaagaaa caacggggaa agtattcgta
     6961 tttacaggtg attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg
     7021 tttctagcag atacctatct ttttgaagga aatgaacatc atcatgccca tttcacttca
     7081 aaagaagctg gagaacttgc gaaatcagct aacgtaaaga aacttgtact tacacaccta
     7141 cctcagtttg gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg
     7201 attgatatag aacttgctgc agttgacaaa gtgtttaaca tttaggagga agaacatgat
     7261 ttttgtacca aatgaaaacc acgacccaag aatcaattta gcaattgaac aatatttgct
     7321 gcaagaaatg cctttagatg aacctatttt gctcttttat atcaatgaac cttcaatcat
     7381 catcggcaga aaccaaaata cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg
     7441 cattcatgtt gtccgtcgct taagtggagg cggagctgtc taccatgatt ttggaaacct
     7501 gaactttagt tttatcatgc ctgatgatgg ggattcattc cgtgactttg cgaaagtaac
     7561 acagccaatc atccaagcac ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg
     7621 aaatgatcta gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg
     7681 tcggatgttt gctcatggaa caattatgtt cgactctgat atcaatgaag tagtcaatgc
     7741 attaaaagta aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt
     7801 aacgaatatc aaaccatttc ttccagaaga aaagcaaaaa atgacaacag aagaatttcg
     7861 tcaagaaatc ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa aaacgtacga
     7921 attgactgat gaagattgga aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg
     7981 ggattggaat tacgggaagt ctccagattt caatttctct cgccaaaaac gtttttctat
     8041 cggttcgatt gaagtccatt tgaatgtctc agaaggaatg atcaaagaag caaaaatctt
     8101 tggcgatttc ttcggactag gagatatctc tgacgtggaa aaaaaattgg ttggacagaa
     8161 atatgataaa gcatctctat caaaaatagt cagccaaatt gatgtgaaga aatattttgg
     8221 tgcgatagaa ccagaagaat tgcttacctt actttattga aaataaaaaa tagcggaatc
     8281 cacatatatg gattccgcta ttttttatac aggattcggg taatttcgag agagttgaac
     8341 agtgatccga tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt aacgatccgc
     8401 tacgtctttc atcatcatta agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc
     8461 tcgactaaat aggtttgtat cgtaatgtgc actatgttcg caacaaatga cgaaatgttc
     8521 ctcttgttcg tgtatcgaca aataaatttt tcgatacagt ggattttcga tggcatgagt
     8581 ttctctaaag gaatgctcta aagcgtggat caaaagttga gccagatcgt catcgttgat
     8641 aggaagttct tcgggaagat ctgcttggaa ggttatctga atattccgtt tggcagctaa
     8701 cttctggtaa cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag aacacagcat
     8761 ttcgaaattt tttgcttctt ctaatatctt ttccacatag tttttagctt ttgtttggtc
     8821 cgtatgcatt aaatcttgca gattctccat ctcttggaca aattccaggc gaagtgtatt
     8881 tacttcttgg atatgctgtc gaagctgctc ataatgttgc tccaacaaat catttttgat
     8941 ttttaagaaa tctacttgtc tccgatcttt gtcggtattt aagatgtgat cgattagatt
     9001 gtagctgata ataacgatta gaatgataat atataaaatc gtagaccaat tgatcgttgt
     9061 ttgatagata cctaatgccg tttggatgta aatcaagcaa tggataatgg ctaccaagac
     9121 gatccaaggt gtacaagcta cataaaaacg gttattctta tatgcttcgc caatgcatgc
     9181 aagtgaagtg actaaagtgc ttataacact agcaatatta gcaatcggca ttaattcagg
     9241 aagttctgca ggagtaaaag ttgcataaag cagaacgccg ataaaaatca aaagttggaa
     9301 cgtacaaaaa attccgtacc attttttata gtaaaacatt cggctccaat agtagcagat
     9361 gatgaaagct gacgttaaga taataaataa atgagataaa aaagaatgaa cgaatggttc
     9421 aaaaagaata ccacttaaaa tatcttctga tacagcttca aatcctaatg ccaaagcaaa
     9481 gcaacttaag agaataagtg agtattcaag acgtttttgt ttatacatca agtagatcgt
     9541 atagaaaaag gtccctatag cgataaaaac agcgattatc atcgtcaata cttgcggcac
     9601 tgattgtgaa taaataaaac taatcaatgg tccagttgtt cctagataaa ctttcggtgg
     9661 cagtccagca tagttctgat aaggagatga gactgtaagg gtcaattttt ttccgatata
     9721 atttgctggt aaatctatca cagccaatga gagtccggga ttgctttctt cctgataatc
     9781 tttccgttgg tataaaatct tttcgcctag atatacagta atccattgat ggtttccttt
     9841 gattagtaaa tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc
     9901 gtctttatcc acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc
     9961 atcttttgta tagaaaaccc acgaatcttt tatttctttc atttgattgc tgtccgcagg
    10021 agaaaaaata aaataaaaga aataagcaat tcctgttaaa aataacaatg ataatataat
    10081 gattatgctc cactttatac gtaaataaaa agattctttt ttattatata tgttattaa
//
LOCUS       DAZKMK010000097        10052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000097 DAZKMK010000000
VERSION     DAZKMK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.099"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..366)
                     /locus_tag="ACG8V1_002383"
     CDS             complement(46..366)
                     /locus_tag="ACG8V1_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0330427.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="ACG8V1_002384"
     CDS             complement(514..2748)
                     /locus_tag="ACG8V1_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0330428.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="ACG8V1_002385"
     CDS             3227..3493
                     /locus_tag="ACG8V1_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0330429.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8V1_002386"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8V1_002386"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0330430.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="ACG8V1_002387"
     CDS             5384..6430
                     /locus_tag="ACG8V1_002387"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0330431.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="ACG8V1_002388"
     CDS             6447..7670
                     /locus_tag="ACG8V1_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0330432.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7759..8646
                     /locus_tag="ACG8V1_002389"
     CDS             7759..8646
                     /locus_tag="ACG8V1_002389"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0330433.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8732..8962
                     /locus_tag="ACG8V1_002390"
     CDS             8732..8962
                     /locus_tag="ACG8V1_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0330434.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9019..9984)
                     /locus_tag="ACG8V1_002391"
     CDS             complement(9019..9984)
                     /locus_tag="ACG8V1_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0330435.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3197 a         1779 c         2034 g         3042 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaaaatcctt gtttctagcg tatactagca tagaagcaag gatttttgat
     7741 ttttaggagg tcgaaaaaat gaaacttggt tttatcggaa caggcgtcat gggaagtgct
     7801 gtcgcacgcc atctactaga agcaggacac gaagtagctg tctataatcg gacaaaagca
     7861 aaagctgatc ctttagtaac agaaggagct atctgggcag atacaccaaa agcggtagca
     7921 gaacaaagta atattctctt tacaatggta ggttacccca aagatgtaga agaaatctac
     7981 tatggacagt cggggatttt ttctgcagat atctcaggac atattttagt tgatttgact
     8041 acaagtacac caagcctggc agaaaaaatt gcaaaaacag caaaagaaaa aggcgcagat
     8101 gcgttagatg caccggtatc cggtggtgac ttaggagcaa agaatggtac actgacgatc
     8161 atggtcggtg gagaagaagc cgtttatgat caagtactgc ctttattcaa agaatttggt
     8221 acgacattta cattgcatgg ttctgctggg aaaggtcagc atacaaaaat ggctaatcag
     8281 atcatgattg caggtacgat gacaggaatg acggaaatgc ttgtctatgc acaaaaaaac
     8341 ggacttgatt taaagaaagt gattgagacg ctttcaggtg gaagtgcagc taactggtct
     8401 ttaagcaatt attctcctcg tattttaaaa gaagattaca cacctggttt ttttgtcaaa
     8461 cattttatca aagatttaaa gattgcatta gaagaagcag aaaaaatgga tcttgtattg
     8521 ccagcaacta cccaagcatt gaaactatat gaagagttgg cagataaagg ttttgagaat
     8581 gacggaaccc aagctttgat caaactttgg tggcctgaag gaaaaatacc agaaaaaaaa
     8641 tcataaaaaa cgagtttttt tagagaaaat caatgcaagt aggctatgct tttgatacaa
     8701 tataacagaa ataagaaaag gaggacctcc gatgacacat tcacaattag tcgcaatcat
     8761 aaaacgactg gaggctatgg tcgagtcagc agataatgaa ctgcaagttc gccgttttga
     8821 aaaagaaggc gtagagaagt gtacagtaac ttatgataaa gcgaccgata catttgaatt
     8881 aactgaaaca gatactcatc agcaatacga atttgataat atcgatatcg tagcgatgga
     8941 aatctatgac ttaatccaat aaattttagg ataattgaca tttgaagttg aagatgccta
     9001 aaagaagccg agaagcaact aatttagttg cttttcagct tcttttttgt ttaacagttc
     9061 aatttcaaac tgttcttttt ctaatttttt gcggtaactg atcaagattt gtacaggatc
     9121 gatttcttct tttgacaatt gacgtttcaa agataaatga gcttctttct tttcataata
     9181 gaagtcagga gaaacgtcac cagaggtaat cattgtttct gcaagttgac cataacaaac
     9241 gaggtagtgc tttttctgac tctcatctgc tgctaatggc atgctgaagg tgagcgatgg
     9301 tgttcttaaa aactgctttt gttcctggat ccgtctaata aaataagtaa ctaattcgat
     9361 atctttttga agcataccgg ctttttcgaa attcaactga tagatgaaat gttgttgtct
     9421 ttttttcaag taagtcagta gctttttatt tcggccttga aacagccctt ggagttcctt
     9481 ttttttctgt tcaaaagtca tttcctgtag gtcaggaagc tggactcgca aagctaacaa
     9541 actaatttca gacaaccaag gcatttgata agtttcttcc attatacgca aaatagacgg
     9601 cattttttta tagagacgaa aaggcccgta tgttcgagcc gtaggagtat ctgttaatac
     9661 aaatccctta ttggtaatat gtacataatt ataattactg aaataattca tttgtcggtt
     9721 ataaaaagga cggtaatgtt ggatcaattg acactcgagc aataaagcat ctaattccgt
     9781 gtctactacg acgaaatcaa aatctgttat atgatgaatc atacgtaata ctttttttga
     9841 atgctgttta ttctgatgaa aatagctgct tactctattt ttgagccgtt ttgctttacc
     9901 aacatagatg accttaccat ctcggtcttt cataagatag acacccggca gcatggggag
     9961 agttttcatt ttttctttta acataggatc tccttttacg ctttgaataa aaagactata
    10021 acaaattttg aaataatgga caagttttag gg
//
LOCUS       DAZKMK010000098         9950 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000098 DAZKMK010000000
VERSION     DAZKMK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.100"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..733
                     /locus_tag="ACG8V1_002392"
     CDS             254..733
                     /locus_tag="ACG8V1_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0330436.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACG8V1_002393"
     CDS             766..2994
                     /locus_tag="ACG8V1_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0330437.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACG8V1_002394"
     CDS             3123..4766
                     /locus_tag="ACG8V1_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0330438.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACG8V1_002395"
     CDS             complement(4839..5384)
                     /locus_tag="ACG8V1_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0330439.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACG8V1_002396"
     CDS             complement(5407..5892)
                     /locus_tag="ACG8V1_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0330440.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACG8V1_002397"
     CDS             complement(5882..6694)
                     /locus_tag="ACG8V1_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0330441.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACG8V1_002398"
     CDS             complement(6833..8365)
                     /locus_tag="ACG8V1_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0330442.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="ACG8V1_002399"
     CDS             complement(8474..9505)
                     /locus_tag="ACG8V1_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0330443.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3318 a         1842 c         1915 g         2875 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat aattcttaaa gaagtattag aaatagttgg tacttcatta
     9901 ctttacatga agatttattc tatggtagga gcaattatat tgagaattaa
//
LOCUS       DAZKMK010000099         9883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000099 DAZKMK010000000
VERSION     DAZKMK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.101"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..329
                     /locus_tag="ACG8V1_002400"
     CDS             <1..329
                     /locus_tag="ACG8V1_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0330444.1"
                     /translation="NGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGS
                     LYRFKGLIALQEQDFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDK
                     LLAESK"
     gene            359..481
                     /locus_tag="ACG8V1_002401"
     CDS             359..481
                     /locus_tag="ACG8V1_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0330445.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            499..2022
                     /locus_tag="ACG8V1_002402"
     CDS             499..2022
                     /locus_tag="ACG8V1_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0330446.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            2046..2285
                     /locus_tag="ACG8V1_002403"
     CDS             2046..2285
                     /locus_tag="ACG8V1_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330447.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(2478..2939)
                     /locus_tag="ACG8V1_002404"
     CDS             complement(2478..2939)
                     /locus_tag="ACG8V1_002404"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0330448.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3134..3352)
                     /locus_tag="ACG8V1_002405"
     CDS             complement(3134..3352)
                     /locus_tag="ACG8V1_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330449.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3470..5086
                     /locus_tag="ACG8V1_002406"
     CDS             3470..5086
                     /locus_tag="ACG8V1_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0330450.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(5327..6667)
                     /locus_tag="ACG8V1_002407"
                     /pseudo
     CDS             complement(5327..6667)
                     /locus_tag="ACG8V1_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7105..7251)
                     /locus_tag="ACG8V1_002408"
     CDS             complement(7105..7251)
                     /locus_tag="ACG8V1_002408"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330451.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(7290..7484)
                     /locus_tag="ACG8V1_002409"
     CDS             complement(7290..7484)
                     /locus_tag="ACG8V1_002409"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0330452.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(7394..7726)
                     /locus_tag="ACG8V1_002410"
     CDS             complement(7394..7726)
                     /locus_tag="ACG8V1_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0330453.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(8240..8431)
                     /locus_tag="ACG8V1_002411"
     CDS             complement(8240..8431)
                     /locus_tag="ACG8V1_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330454.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(8525..8911)
                     /locus_tag="ACG8V1_002412"
     CDS             complement(8525..8911)
                     /locus_tag="ACG8V1_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0330455.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            9605..>9883
                     /locus_tag="ACG8V1_002413"
     CDS             9605..>9883
                     /locus_tag="ACG8V1_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0330456.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKV"
BASE COUNT         3233 a         1550 c         1870 g         3230 t
ORIGIN      
        1 ataatggcaa atactatcat caacaccatc aagtacatga gtttaaatca attctcttaa
       61 cttcagatca agacataaaa aaagattttt tactccaatg gattgactgg ctgattttaa
      121 ccaaccaagg gagcctgtat cgttttaaag gattgattgc tttacaagag caggatttta
      181 tagttgccat gcaagaggta aacgaacagg ttaattttca atacaccact cgacaaaaag
      241 accaaaccac tattattcta atcggaaaaa tgttggatga agaaaggatc aaacagagtt
      301 ttgataaatt actggcggaa agcaaataat gataagtagt gattgggggt aatttctaat
      361 ggagaaaacg gatatatcta gtgcatatcg acgacttaaa agcccgaata ttaaaactag
      421 aaaaagggca ttaaaaatta tcaaagaaca taaacgaaat aaaatgaaga aattagcata
      481 attaaggaag gaaaaagaat gaagaaaatc agtgtaggac ttattgggat agcagctcta
      541 ggattattgg gagcttgctc cagcactaat gatgcaaaag tatcgaatga taaagatgga
      601 aaattagaaa ttgtaacaac attctatcca atgtatgatt tcaccaaaaa catcgtcggc
      661 gatgaagcga acgtggattt aatggttcca gcgggatcag aacctcatga ctatgagccc
      721 tcagctaagg atatggcaaa agctcatgat gctgatgttt ttgtatatca taacgaaaac
      781 atggaatcat gggtaccaaa agctaaagaa agttggaaga aagctgggcc taatgtagta
      841 gaaggaacaa aagacatgat tttattgcca ggtagtgaag aagaagatca tgatcacgga
      901 gaagaagatc accatcatga actagatcct catacgtggg tttcacccaa aatggcaatt
      961 aaggaagttt caaatatcaa ggatcagtta gttaaattgt atccgaaaaa agcaaaagta
     1021 tttgagacaa atgctgaaaa gtatttgact aaactgaaac ggttggatgc tgactatacc
     1081 actagtctta aagaagcgaa acaaaaaagt tttgtgacgc aacacgcagc ctttggatat
     1141 ttagctttgg attatggatt gaaacaagtt cctattgctg gactaagtcc tgaagaagag
     1201 ccatcttctg gtcgattagc tgaattaaaa gagtatgtta aaaaaaataa gatcaattat
     1261 atttactttg aaaagaatgc taatgataaa attgcaaaaa ctttggccaa cgaagctggg
     1321 ataaaattgg aagtactgaa tcctcttgaa agtcttacaa aagaacaaat ggataatggt
     1381 gaagattatg tttcagtaat ggaagacaat ctaaaagcgt tagaaaaaac aactatggtt
     1441 gctggtgaag aggtagttcc agaaaaagaa gcgaaggatg aaaagacagt tgctaatggg
     1501 tactttaaag atgccgatgt gaaggatcgc gaattgagtg attatacagg cgaatggcaa
     1561 tctgtttatc cgttgttaaa agatggaatt ttggatgaag ttttcgatta caaagcaaaa
     1621 atcaataaag acatgacagc tgccgagtac aaagattatt atactactgg ctataagaca
     1681 gatattgata ctatcaacat taaagacaac accattgatt ttgtagtcaa cggtgaacat
     1741 catcaataca catacaaata tgttggatac aaaattttga attatgaaaa aggaaaccgt
     1801 ggtgttcgtt ttaattttga aactgatgat gcaggtgctg gtcgttttaa atatattcaa
     1861 tttagtgatc atggaattgc accaagtaag gcagagcatt tccatatttt ctttggtgga
     1921 gaaagtcaag aaaaactcta taatgaattg cataattggc ctactttcta tccagcttca
     1981 ttgtctaaac atgaaattgc tcaagaaatg atggctcatt aaaattcaat ttttggagga
     2041 aatacatggt aagaaaagta ttattgtcta tggattccaa gaatattgat gcttatcggc
     2101 gttatcgatt tgcaaagcaa atgagattaa atgggagtac attagctgat attgctgcaa
     2161 ctttacatgt tagtattgac acagttcata gagtattaaa acgggatcct gattgttgtt
     2221 ttatcaaggt caatactgga agaagtagac gtttagcaaa acggcataat agcttggcca
     2281 attaattgat aataccagag tagatcattt ttcccttatt tggatttgat ctgcttttta
     2341 ttatgtcttg aattgtcaaa tcaagcgcaa cactttatca aagaagactt cataaggtgt
     2401 tctccaatct aaacactttc taggtctgtt attcagtttt tcaatcatct gatttatcgt
     2461 tgagttatct accgtagcta tctctttcga ttttggcaag tattctcgaa tgagtctatt
     2521 cgtattttaa tttgttcatt gttgccaagg gcatgaggat ctggaaaata aaagggcata
     2581 ttattcaatg cttctgtaac tgcgaaatga ttggagaatt cttttcctct atctggagtg
     2641 atcgttttga ccgcctcatt tggaatactg cttaaaagtt caatcatttt ctcgctcaca
     2701 taagcggctt tctttctggg gaccttcttt cctgctaaaa ggaagcatga ttttctgtct
     2761 gtcaaagtca ctaaacaagt accaccagtt ttgccagcta caatatcggc tttccaatga
     2821 ccaaaatcac ttctatcatc tgcggatttt ggacggccat ggatcgaatg agtaaggcaa
     2881 atctttccac gagactcgac atgatttttt gtatgtcttg tttttcctcg atggtgcaat
     2941 gaacggatca ggcctttatt gccgtgtgag aggggttctg tatcgaaaag atcattgcaa
     3001 attgcacgtt agatcgtgat aaaactaatt gaaaagtcca tatgttcatg cttcaaacga
     3061 ttcgagattt cctctggcga ccactgaaaa tcgacaaata aatgacgaac aattgaccaa
     3121 actcacggat catttagaat gtgataacgt ccgcattttt ttcttcgata agcataattt
     3181 ttttccgctt tggaaggtga atagtttttt tcttgtttct tctcagttca tgtaaaatag
     3241 taaacgggct atgctgaatg tcgttgccaa ttatactaat agaaaatcct tcttcgtgaa
     3301 gcagaaagat tctttctcgt tcgagtatag taagatgacg atactggctc ataattattt
     3361 tccttctagg agtgttctcg acaaacttat actagttgga tatcgattat gactcatttt
     3421 tatgtgttgc acttaaagtg taaattcaag gtatagaaag aggttcccta tgaaaaaaat
     3481 acctattaca atcttaactg gctatcttgg ttcaggaaaa acgatacttc taaatcatat
     3541 tctgtacggt gatcacggaa aggaaattgc tgtcattgta aatgacatag gggaagtaaa
     3601 tgttgatggg gtattagtcg acaagaaagg ttttaaaaga actggagaaa aacttgtttc
     3661 gatgccgaat ggatgcattt gttgtacatt acgcgaagat ttgattgttg aactagatca
     3721 attagtgcaa ttttctgact tgaatcaaat tgttattgaa gcttcaggaa tcagcaaacc
     3781 aattccaatt attcaagcaa ttatattagc taaaaaccct ttagacattg atttgaccca
     3841 acgattagag attggtgcca ttattatagt agtggatgta aaacgttttt gggataattt
     3901 cgaaacgggt gaaaagattc aggaaagaaa agctgaaggt aatgtagttg aagaagaaca
     3961 ggatattcgt ggcttgttaa ttgatcaatt cgagtgttgt aacatattac ttttaaataa
     4021 tagtgactta gtgtcaacca agacgattta caaaattaac ggtctaattg ctaagttaca
     4081 gtccaatgcc aaaataatta ggacaattaa aggtgaagta gacttatcga aaattctaaa
     4141 tacaaatctt tttaatatag atgagacttc ctattcagtg ggctggttca aagagttaga
     4201 gcttggctat caaaatcata ttcctgaaac agaagaatat ggtatctcaa gttttatcta
     4261 tcgaaatcgg ttactccttg acgcacaaaa atttgcccat cttacggata aaaattttcc
     4321 tggcaatatt acaagagcaa aagggtttct ttggtttgcc caacatagtg atttttcgac
     4381 gatctttgaa acatcgggta gctataaaag tattgaatca ttaaattatt gggtagctag
     4441 tttacctaaa attgagtgtc aaatgattat acaaaaaaat cccgaattga aggaagcctg
     4501 gcatccgttg tttggtgaac gcattaataa actagttttt attggtatga agatggatca
     4561 acaatgtatt aaggaacaac ttgagggttg tttaactgat tctcttaatg cagataattt
     4621 ccgaaaaatt tcaccatttc catggaaaaa gtctaatatg tttagccaga tatcgttaat
     4681 gaaattttta agaaaagctg gtgtcaagat aggcgatgtc ataattattg gcattttgat
     4741 tattacctct tttattccct tatttgtatt ctctatagaa aaggagaatg aaacaactga
     4801 agatgctatc aaatatgcca tcgttcgaat tgatggcaag gaaatggatc gcttcgattt
     4861 ggataagatc aatcataaac tcgtgaatta tcatcctgcg aagggacaat ataatattgt
     4921 tgagattaaa gatggaagaa ttcgagttaa agaagataat agtcctgatc aaatcgcggt
     4981 tagaactgga tggatctcaa aacccggtca aacgagtatt tgtttacccc ataagttagt
     5041 tattagtatt gagaaaaaag aaagcacaga ttactacatt tactaatctg gaaatagatg
     5101 aggaggtttt taataagaaa aagatattat tattaataat agcaggggct ttattaagtg
     5161 tgttaagcgc ttgtggacaa gaaaaagaag cggtgacaac aggcacaagt atctttaacg
     5221 ggacctgcaa tggtttagtt aacagtcggt tagattactt attttggctc ttcgtcaatg
     5281 agtgttggtg aagaaattca atagaataat cgagatactc acacggctag acagctatct
     5341 tgtctagccg tgttgttgtt ctggctttag tcagttgggt agtattctta gttttcttaa
     5401 aatattttcc tcgatataaa aagattcgtt ctcggtagtt ttgaaagttt ctatagccat
     5461 aggcaattcg ttttagtact ttgatatggt tattcaaaca ttctataggg ccgttggaat
     5521 agggaacgcg caacgctcgt ttgatttctg tttggtattt cttgaaggtc gtgaacactg
     5581 gttggtagtg ctctggcaac tccttgtagt cttcttttag gagcgcgacg aattcaggaa
     5641 catcttgtct tctgataacg gaaagaaaat cctggtagac ttcgtagcct cgctttaagg
     5701 aatcatcata agataatagc cgatcgacta tctctgcttc tgtcagatga tctcgaaatg
     5761 acggacgcca gtagcgatgc tgacctttca attcccacgc attcttttga agtagtttcc
     5821 agtatttctt taagtgcttc gaccgacgat cccctttttt aaaggtcttc attacttgaa
     5881 ttcggtggtt ggtaaaagcc cgaccaatgt gttggctaat atgaaagcga tcggcgacga
     5941 ttcgggcatt cggaaaacac tctttcacca atgtgcgata gggtgtatag aaatctgaaa
     6001 ccactagacg cacttgttca cggactttta tagggtagcg caagaaatag tcgcgtaaag
     6061 gattgagttg ccgattttcc acaatatcaa tcagttgatg ggtcttgccg tccatcatga
     6121 caaagctcat ggctcccgag acatttttca ctgatttgaa ttcatcgaaa cacaccactt
     6181 ctggtaacag ctggctcaaa gaaatggttt taggccgaag gctgcgaagt attcgaatga
     6241 cagtggtcgg agaaatgtgc ttcatacgtg cgatcaaaga catagaaaga ggttcgctta
     6301 atagctcaag aatggcttgt ttgactctgc gagcaatgga gcagcgacga tcactaactg
     6361 aatcttctgc taaaaaagtc tttccacagt gtttacattt gaagcgctgt ttgttaatcc
     6421 gcagaatggt cttgtagtta ctgacatcat tcaataagat ggatactttt ttccagccct
     6481 atttaatgat cgtttgttca ttttgatagt ggcgatgaaa gcattcctcc ggttgatagg
     6541 tcaatgtgcc atcaaaaaca aaacatgttt cgtcattgat tcgctctttg cgaaaggcat
     6601 cttcatgaaa gataagattt aggtctagaa tatctagtaa ctctttggta aactctgaat
     6661 aggacatctt gaaacactcc tttgaatggt tttcgtcgac tttattctaa aggattttaa
     6721 ggtgtctgtt tgcgtttatt agaaaaaaga aaaagtattg gcaacagaag gcgtcaccaa
     6781 cactagatat tatagatccc ttattttctt cgcttcaaat tgtcaaggag tcatcataaa
     6841 ttttgtatgg attcttttgt ttgggtagta gtcttctaaa taatcttgga ttaacacctt
     6901 catctctatt ttacttttat atttgttgga gtcgattaat tctcatttga ttgatcgatg
     6961 aaacgcttca attactgcat tgtcaacagg agtcccagtt tggctcactg aatgtgtgat
     7021 gtggtggtcg ttcaacagct cttgataaat aaaagaccga tgctggctac ctctatcgga
     7081 atgaattacc gtaggcaatt ctccttaata aatttgtaat ggacggacga caatctcttt
     7141 tttcatgtgt gaatctacca tatagtaacg tagaatacga gttgctaagt caatataggt
     7201 ggacaaatag aggcgaccgt cattacatgg aatgtaagta atatccatca cccaaacagc
     7261 atcaatttgg ttctgttcaa attatcgatt caacattttt ttaccatttc tttgattgtt
     7321 ttttctctac catgagaaga tttgcgtcga tgaaatcctc ttgtgcataa attcatttca
     7381 cgcataagaa cagctacaac acgtttattg atttcgattc cttcatcgta aagtttttga
     7441 gtgatccctg gagatccgta tcgatctttg tgttcgtaga acactcgtct tcctctttta
     7501 gtcgtttatt ttcctgttca agaatgtaga aatctgcttt atcaggtaat ctttttcctt
     7561 gtccagcaaa agcatttttc ccgtatcgac gatattctga aacccatcgg gaaattgttg
     7621 gacgagagat attgtattct tcagcaattt tcttggtagt tacttttttc tcaaggatat
     7681 cctgtgaaat ctttagttta aatttacgat catatgtttt tctcaataga gttcattcct
     7741 tccggtaacg taactgatag tcaagaaaag tgtatcatag cccaacttag tatcagggtt
     7801 tgtcaactaa actgtggaag ttgatagata agagttttta acaaccaaaa ttctcttgac
     7861 tatttttgaa atcagacaaa ttttgattgg acacatagtt gaataaatct accgtcgtta
     7921 cggaaggtaa atcgcatgct tttcaattct agaggagaag tcctcgttga agtaccatag
     7981 cccagttctg gattcgacct cctgcccact tcatacgtaa ataaagtaat tttaggagtg
     8041 agactgtgac aaaagttatt tttaagctaa aaacgaatta aaaaacgttg ataattaaaa
     8101 tcattcgttt tagattctat gaatcattat agaacgctgt aaaatcgata ctttataatt
     8161 ataaagaaga tagtttcaaa tttattgttc gaaatttgaa actatctttt tacttatgtc
     8221 acagactccc ttagttgagt tattctttaa tttcgaattt cgagatagct agatatattg
     8281 caagcataca ttcttgataa caatcttgat catattttcc agttctccag gattgcttat
     8341 gaatcaaaga gctaaatctt ttgagaattg cctcaacagc ttcttcattt ccagcctttg
     8401 ctaaaatgaa atcttctttt aaagaattca taaatctacc ttcttcccat ggacttataa
     8461 tctatttctt tgggaaggag gagaacgagc aaagaagcat acaaaattag ttattatgca
     8521 gatattaaac cagcattttt tgaatttttt tgtaaagtcg atgttttaaa tttgtcgcac
     8581 cctgtcttgt aatccccaga acctttccaa tttctttatc agttttatca aagataaatt
     8641 tttcgataag aaaaatttgt tcaggttctt tcaacaaagg aaggattttt gctaatttta
     8701 tatcttcaac aaggagagtc acatttaaaa cattaatcgg ttgtttaaat tttatagtat
     8761 ctttagtaaa caaatatggt gggagttgat caagttgttc atgtttttga atttcttctt
     8821 gttttttata ataagtatta gcggtgttca aaatgacctt ttcaaagaat cgaatcaact
     8881 gggcatttac gcgttcgtcg ttatctgaca tattgcacac ctcctccatt aagatttaaa
     8941 gggaaaagag gcaaaaaaca cgaaaagaag ctaatgcaat tatgctgcgc tgcgaataac
     9001 ttctataagc tcacatgcat tgataggact gctgatgttt attttcaaaa atgttttttt
     9061 agttgataat ggatagttat ctttatactt agaaaagaaa attcatatat cttcttaaat
     9121 atcaaacatg tccttatggc taagaaaatg tcttgagctt tttacgtata ataaatcttt
     9181 tctaaaacct ccttttggat aatattctta ttcgtgtata gattgtattt tttatttctt
     9241 cccccttcat aattaacaac tagaaaagag ttcgaataga cacgtatttg aacgaaaatt
     9301 tttattggat tcccaaattg gaatatttag aaacgaaaaa ttcctattat ctgtatagtg
     9361 cttagataat aacggatcat aataattatt gtctttgaac tagatatatg taaatagtat
     9421 tcatcatgat gaaaaatgat gagttctcgg tgataaaatg tactttttac ggtggaatat
     9481 acatgaaagc gataagtgga tattttataa tgaagtaagt tagcttattt tgacagagga
     9541 atagtatttt agataacgta taaaatacta tttaatgatg tagaaaggaa agagagaaac
     9601 aaaagtgttt tcagataaag aagtcttatt aaagtctctt catgtttgtt taaagttacc
     9661 tatcgtggtt tttaattccg aattcagtat tataaaagag tatcgctcag atagaactac
     9721 ttattttttt tatgattata aaaagttact aactgataat attaaaaaaa ctaaagattt
     9781 ccatttcttt actggtgatt ttaatgagat gtttcttcta tacatgttta aaaccagata
     9841 ttacttattt ggtccattta gagcaaataa tattgataaa gtt
//
LOCUS       DAZKMK010000100         9855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000100 DAZKMK010000000
VERSION     DAZKMK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.102"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..2196)
                     /locus_tag="ACG8V1_002414"
     CDS             complement(76..2196)
                     /locus_tag="ACG8V1_002414"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0330457.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2426..3604
                     /locus_tag="ACG8V1_002415"
     CDS             2426..3604
                     /locus_tag="ACG8V1_002415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0330458.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3620..4378
                     /gene="aroD"
                     /locus_tag="ACG8V1_002416"
     CDS             3620..4378
                     /gene="aroD"
                     /locus_tag="ACG8V1_002416"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0330459.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4401..4886
                     /gene="ybaK"
                     /locus_tag="ACG8V1_002417"
     CDS             4401..4886
                     /gene="ybaK"
                     /locus_tag="ACG8V1_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0330460.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4957..6210)
                     /locus_tag="ACG8V1_002418"
     CDS             complement(4957..6210)
                     /locus_tag="ACG8V1_002418"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0330461.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6327..7676
                     /gene="gdhA"
                     /locus_tag="ACG8V1_002419"
     CDS             6327..7676
                     /gene="gdhA"
                     /locus_tag="ACG8V1_002419"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0330462.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7788..9134
                     /locus_tag="ACG8V1_002420"
     CDS             7788..9134
                     /locus_tag="ACG8V1_002420"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0330463.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9442..9828)
                     /locus_tag="ACG8V1_002421"
     CDS             complement(9442..9828)
                     /locus_tag="ACG8V1_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0330464.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3141 a         1792 c         2015 g         2907 t
ORIGIN      
        1 ttgattttgt acaacccatc agtccttatt gacaaagagc ctaattttgt cgctgctcct
       61 tttaaattta ttttatcatt cggaagaact tgtggtcgtt ccgtgaagtt cttggtacgt
      121 ttgcgtttta taaagatcaa cagttgattg tccgttgtgt tcactaaaga cactagttga
      181 cttgtcacca agagattttt cagcttcttt taatttctct aagccattct tgtagtcata
      241 atcactggta tcaattgggt ctaatccact attcgtatag aaccgaagaa gatcaccatt
      301 attgatttgg tcagaaacag ctaactgttt atcaactttt tctttcagtg aatcaatttc
      361 attctgtact tcctcagtag gttcagtaat agcttcacca gtttcgttac tatagatcgt
      421 accaccgtag taagtatatt ttggtgtaac aaaatcacca tcacggaaag aaacaagctg
      481 ttcatgatct ttcgagaata gatcttgtcc taactgaata taattttttg tatctacacc
      541 tagtaagtga agtaatgtag gcaaggcatc aacttgtccg ccgtaagtgt gattgattcc
      601 gccctgtgtt tgtccaggga tatggatcat ataagggaca cgctgcattt gtgcattgtc
      661 gtagtttgtc caagtagaac ttgtttttcc aactaattca gctagatttt tatttcttga
      721 attcgagaca ccgtaatggt caccgtaaag gacgatgact gaattatcat acaagccgct
      781 ttctttcaaa taattgaaga attcttgtac ggcggtatct aaataattgg ctgtcgcaaa
      841 gtaaccgttg atcgtttcat catttgtatt ggctaacggg aatcctgctt cgtcattggt
      901 gaattttgaa taaggataat ggttagatac agcaatgaat tttgaataaa atggttgttg
      961 cagacgttcc aagtattgta cggattgttg gaaaaatggt ttatcatgca acccgtattg
     1021 gaacgaattg tcatcattaa catcataata agaagaatca aagaaataat catatcctaa
     1081 attcttgtat gtctcgtttc gattccagaa tgtaccagcg ttcccatgga ataccgcact
     1141 aatgtaacct tgtgtttgac ttaaaatatc aggtgcagct tggaaagtat tttttccgcc
     1201 taattgtgtg aacaaggagc cttgattcaa gccaaacaat gaattttcca tcagcgtttc
     1261 cgcatcgctt gtttttcctg cttttacttg atggaaaaag ttgtcaaaac tgaacgtgct
     1321 gttgctgtgg taaagactat tgatgaaagg tgtaacttca tgctggagac cattctcatc
     1381 tgtcaattta taatcgatca ggaattgctg gaagctttct aaatggatat aaatcacatt
     1441 ttttcctcta gcgagcccga acaaatcgtc atttggtgct gcataatgtt cttttacata
     1501 attttctact tctttcatat cattgggact tgcttctgcc cgcacttggt ttgtctggta
     1561 agtttgaaca ccgtcataaa ccatgaaagc attcaagcca agatatttga ccaaataatc
     1621 acgagagaat gttcgagtca gcaattctgg acgatcagct tccgccataa aaagatttcc
     1681 tgagaaaatc ataactgcta atgtagagat cgcaaaagcc attcttgctc ggatcgggcg
     1741 agggtccaac ttgatttttt ttgcaatcag cagaacagtc aagataataa aatcgatcca
     1801 atagatcaca tcatatgggc gaaacagtcg aatggcactt tctcccagac cactcgctac
     1861 tttcccagca cctaaaatcg tgttgatcgt aatgaagtcc gtgaattctc gataataagt
     1921 gacattcgaa aaaacaagca atgtcaaaag aaaataaatg accatcatcg tgatataagc
     1981 aactctaggg cgtctgatat aaagagcaat tccaagtaat aacaatgtag ttgcaatagg
     2041 gttgataaac aagataaagt attgaatgcc actttcgatt ccgagatgga aatcaactaa
     2101 ataggcaaaa atcgttttca accaaagtaa aacgactagt aaagaaaaaa atccgagcct
     2161 cttattcaaa aaggcaggtg tttttataca tttcaagata atcttccttt ctgcacttct
     2221 taatcacaga agtgtcctga aaccatgtta cacagctcac ttattctcat ttattttact
     2281 agtatgacaa tatagtgtca attgaaacca gaagtttatg ctgtttcatt aaggttttct
     2341 ttaagcttca tgaaaaaatg agcagtaaga caagtcctat gttttttgtt atacttggga
     2401 agagaaagct aaagcaggtg aaggaatgaa agtaaaagta aaaaaaagag caagcaatcg
     2461 attgaaaaaa ggatatccac tgatccaaaa agaagacctt gctcatcccc aaaaagaaac
     2521 cacgcaatgg gtggaattag ttgattcgca aaaccaattt atcgcaaaag gatatctagg
     2581 aaagcagaat aagggaatcg gttgggtatt gagccagcag aacatcccat ttgatcaatc
     2641 tttttttgaa gaattttttg tgaagtcaaa agaaaaaaga actttctttt tttcagatga
     2701 ttctactacc gcttttcgta tattcaatgg agaaggagac gggatcggcg gtctgattgt
     2761 tgattattat gcaggttatg ctgttttttc ttggtataac gaaacgttat atgcacatcg
     2821 tgaattattg attcaagctt ttcgtgaaat ctacccggaa atcataggag gctatgagaa
     2881 aattcgattt gaatcagtaa atctgcctga gagccagttc atttacggaa aagaggcacc
     2941 ggaacccttg atcgtaaaag aaaacggagt aaaatttgcc gtttatttaa atgaaggatt
     3001 aatgactggg atctttttag accaaaaaga agtacgggga tcattagcag aaggtttcgc
     3061 ttctgggaaa acagttttga acatgtttag ttataccggc gccttttctg ttgctgccgc
     3121 aatgggaggg gcaatcgaaa caacaagtgt cgatctagca aaacgcagtt taaaaaaaac
     3181 acaggaacag tttgaagtga atggtttgga tgtcgatcag caaaaaatca ttgttatgga
     3241 cgtattcgag tatttcaaat acgccaaaag aaaacaattt ttatatgaca tgattgtatt
     3301 ggatccacca agttttgcca gaaataagaa aaaagtcttt cgcgtagcaa aagattatgg
     3361 ggaactggtc aaggatagtc tggatatttt agcagaagac gggatactta ttgcgtctac
     3421 aaatgctgcg aatgtctcga tagaacaatt ccaaacaatg attgaagagg aattccatgc
     3481 caaaaatgtt tcttttgaac aacttgcttt atatcgtctg ccaaaagatt tccagacgac
     3541 ggataccttt ttagaaggca attatctgaa agtgtttgtt tatcaaatta aaaataacca
     3601 ttagaaggag cgatgcagaa tggttcgggt aaagggattg aatataggaa gcggtagacc
     3661 aaagatttgt gcgcctctta tcggggccgt ccgagaggaa gtattacgtg aagcagttct
     3721 tgcacgtgaa gcaggggccg atttagtcga atggcgagtc gatcattatc gtgaagtttt
     3781 ttcttttgat gaagtagcag aaacattggt tcttatccat gagatgcttg atggactgcc
     3841 gttattattt acgtttcgta ccttagctga aggcggagaa caagacatat cgatccgtca
     3901 gtatcgcgac ctttatgaac atgtgataca cacaggatta gtagatatgg tcgatatcga
     3961 gttgttcaaa gtagaaagtc taggaaaaag actgatagag gaaatcaagt ctttaaacat
     4021 tcctttaatt atcagtagtc atgattttaa agaaacacca gcagaccctg tcttgcttta
     4081 ccgattgaat gttatggaac attttggagc cgatatcgga aaactggcag taactcccaa
     4141 taatgaacga gatgttttgc ggttgatgga gttgactaga cgggcaaatg cttttttctc
     4201 catgcctatt attacgatgt ccatgggaaa tcttggtaaa atttctcgtc tggttggcgg
     4261 aacgacgggt tccgttatga catttggttc cttaagcagc gattctactt ctgctccagg
     4321 acaattaccg gtcaaagcat tgaagcaaat tattcaatta ttaaatgaac cgttgtagaa
     4381 caagtttgag gtgatatttt ttggcaaaga aaaaaatagt aaaaacgaat gcaatccgta
     4441 tggtcgaaca aaaaaagatt ccctatacag agcacgaata tgagtgggac gaaagccacc
     4501 taagtgcgtc atcggtcgct gaacagcttc cagaaagcca atctcgtata tttaaaacac
     4561 tggttgctgt gggcaatgtg acggggcctc ttgttgctgt gataccggga gaagccgaat
     4621 tgaatctaaa aaaattagca aaagtttcag ggaataaaaa agtagaaatg ctccatttaa
     4681 aagatttgga agcaacgacc ggatacatac gtggtggctg ctcgcctata ggtatgaaaa
     4741 aactatttcc tacttattta gatcaaattg cagaaagcta tgaacaaatc attgtctcgg
     4801 ctggcagaag agggctacag atggagttag cacctcaaga tatctgtgca ttaacgagcg
     4861 gacaatttgc agatataaaa caataaatca tacgtataaa agaaagaagg aggccgggat
     4921 gttgtttgtc cagtctcctt ctttcttttt tatacattat gctctttgcc cggcattcat
     4981 tcctgctaca tgccctgtag cgaaagccgc tgtgatatta aagccgccag tataaccatt
     5041 gacatccagt aattctcccg caaaatacag accatcaatg attttactct ccaatgtttt
     5101 aggataaatt tcctttagag aaacccctcc gccagttaca aatgatttct caataggaaa
     5161 agttttgttg attgatagct taaactcttt gcatagttga acaaaatctt gaatttgttt
     5221 ttgagaggct tgttgtcctg tcagatgatc catttcaagc cgctcaagaa agaaaactaa
     5281 caatcgctca ggcaatatac catgccaagc attgaccaag ttctttttgg ttgctttact
     5341 tttttctgtc aatatatgaa taagctcttg tttggtttgt gtagggaaac aatccagaga
     5401 aagtgtcact ggttctcccg ttttctctaa ttctttattg acaaaacttg aacaacgcag
     5461 tgcagccgga ccagaaatac cgaaatgggt gaaaagtaaa tccatcgtat gttcagtcaa
     5521 tacacgacct tttttattta aaacgcgtaa agtgatgtct tgtaaagaaa gcccttgaag
     5581 tgttttctcc tgaatatacg gttcatccga tatcaagggg gactctgtcg cataaagagg
     5641 cgtcaccgtg tgacccactc gcttcacgat cttgtaccca tctccagtag aaccagtaga
     5701 tggataagtc cgtccaccag ttgttaagat aatggcagga gcatggaact cttcgaattc
     5761 cgtgcgtata ccgtaaattt tatgttcttt gtggatcaat ttctctactc gagtagagaa
     5821 tagaagcgtc acatctagtt ctttcagacg attgagcaat gcttcgataa tcgtttttga
     5881 tttatttgtt acaggaaaca tccgtccatg atcctcttct tttaaatgta cgccttgtga
     5941 ttcaaagaag ttcatgatat cgaaattatt ccattgcgca aacgtactat ataaaaactt
     6001 tccatttcca ggaatatgtg caatcaaatc atcaacagga cgattgttcg taacattgca
     6061 ccgaccacca cctgtcatca gtagtttttt tcctgctttt ttgttttttt cgatcaagag
     6121 tacgcgtgcg ccttgttcag ctgcactgat cgctgccatc atcccgcttg ttccagcgcc
     6181 gactacgatc acatcataca gtttttccat tccctcacct caaggaaagt gtagcatatc
     6241 tgaagaaaga aaacagaaaa aaatgttttg agccaaaaga gaaaatgagc taaaatgagg
     6301 gaagataagc aaaggagcgt ggaacgatga caaacgcaac agaatatgtt caagcaattc
     6361 aagaaaagct acatcaaaag gatcaagggc aagaagaatt tttacaagca atcgatgaat
     6421 tcatgcccac ggttatgtct tttttggaca ctcatcccga atatatcgaa aagaatatct
     6481 tagaactttt aacggaacca gaaagagtta ttcaatttcg agtgccttgg caagatgatg
     6541 aaggaaactg gcgagtaaat agagggtacc gtatccaata caactcagct atcggccctt
     6601 ataaaggtgg attgcgcttc catcctagtg tcaatctaag tatcttgaaa tttttggctt
     6661 ttgaacagat tttcaagaat agtttaaccg gtctgccaat cggtggagga aaaggcggaa
     6721 gtgacttcga tcctaaagga aaatctgata atgagatcat gcgtttttgt caaagtttca
     6781 tgctggaatt gtcaaaacat atcggcccat cgattgatgt tcctgcggga gatatcggtg
     6841 taggagctag agaaatcggc tatttgttcg gcgaatataa acgattgaaa aaatatgata
     6901 ctggtgtgtt gacaggtaaa cctttggatt tttggggaag taagatacgt acggaagcta
     6961 ctggttatgg attagtgtat tatgtcaaac atctattgaa cgaagaaaaa gattcattta
     7021 atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc catttatgca atcgaaaaag
     7081 tgcaagagct aggcgggaaa gttgttacat gttcagattc tagcgggtac atctatgatc
     7141 cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga aatcaataga caacgtttga
     7201 cggaatacgc aaaccaacgt ccttctgcta tttatcatgc tggagaatct gtttggacat
     7261 tgaaagaaaa agcggatatc gctttgccat gtgctactca aaatgaaata gacgaaagct
     7321 tggccgaaat cttagtagaa aatgggatca aaatcgtagc agaaggcgct aatatgccat
     7381 gtacagtaaa agctgtttct gttcttcatg aagcaggcgt gtggtattgt ccaggaaaag
     7441 ctgctaatgc tggaggagta gcagtatctg ctttagaaat gagtcagaac gtccagcgat
     7501 tgacttggga aagagagcaa gtggatgagc agctggatca aatcatggaa acgatttacc
     7561 atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa aaatctgctt cttggagcaa
     7621 atgttgctgg atttgaaaaa gttgccaaag caatggcagc agaaggttta gtttgaaaaa
     7681 tgaaactttc ttggaaatat tgtcagcatt ttgtttattt gcttgaaaaa taataaaaaa
     7741 aaacgtaaag tattacctgt agagtaaacc tataaggagg agaacaaatg gcacacattc
     7801 attttgacta ttcaaaagta gcaccgtttg tcaacgaaca agagttaggg tacatgcaaa
     7861 gccaagtaac agcagcccac aacgaattac gggaaggtac tggcgcagga agcgacttcc
     7921 gcggctggat cgatttacca acgaactatg acaaagaaga atttgcacgt atcaaagaag
     7981 cagctaaaaa aatccaatct gattctgaag tattagttgt tatcggtatc ggtggttctt
     8041 atttaggcgc tcgtgcagcg atcgacttct tgaatcacac attcttcaat ttattagata
     8101 gcaacacaag aaaagcacca caaatcttct ttgccggaaa ttcaatcagc tcaacatata
     8161 ttgctgactt gatcgaagta atcggtgatc gcgacttttc agtaaacgtt atttcaaaat
     8221 ctggaacaac tactgaacca gcgattgcgt tccgtgtatt caaagagctt cttgttaaga
     8281 aatatggaca agaagaagca aacaaacgta tctacgctac aacagataaa gcaaaaggtg
     8341 cagttaaagt agaagcagat gcagaaggtt gggaaacttt cgtgattcct gatgacatcg
     8401 gcggacgttt ctctgtatta actgctgttg gtttgttacc aatcgctgtt agtggtgcag
     8461 atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa agcatactca agcgacaaac
     8521 tagaagaaaa cgaagcttac caatacgcag caatgcgcaa cattctttac cgtaaaggca
     8581 aagtaactga gttgttgatc aactatgaac caggaatgca atacttctca gaatggtgga
     8641 aacaattgta tggtgaatct gaaggaaaag accaaaaagg catctatcct tcaagcgcaa
     8701 acttctcaac tgatttgcac tctttaggac aattcatcca agaaggacgt cgcaatattt
     8761 ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat cacaattcct gaacaagcag
     8821 aagatttaga cggtctaggc tacttgcaag gaaaagaagt ggacttcgta aatacaaaag
     8881 catttgaagg aacattgctt gctcatacag acggtgacgt gcctaacttg ctagtcaaaa
     8941 ttccagaaat ggatgcttat acattgggtt atacaatgta cttctttgaa attgctgtag
     9001 gtatttcagg ttacttgaat ggaatcaacc cattcgacca accaggtgta gaagcttaca
     9061 agaaaaacat gttcgcactt ctaggcaaac caggctttga agatcttgct aaagaattga
     9121 acgaacgcct gtaagataag ggtttttcgc tagatttaaa taatcagtga tagaaacatg
     9181 ttcgctttaa aatgaaaact ggtacccatt taggtactca aacatactgc atcgtttatt
     9241 cagaataagg ggctgtgaca agacgtttgt cacagtccct tgtttgtata gaaatcgatt
     9301 gattgccttg ctctttagtt tgttttagga ctttaagaac agtgaggctg acttcgaagg
     9361 ttaattctgt ttttttgttt tctagaatca gtaagcaaaa aagcactcac ttcgagtgct
     9421 tttttgcgat atattcttag cttaagatag gcctaatttt tcttttacag atgagggtac
     9481 cttatcgctg gtaattactt ttttctctat gatccgcttt tcgcttgctt tcaactgcaa
     9541 gatcgttcct tcggggtaag cagaaccatt ggcagagtca ccagaagtaa tgacatcgaa
     9601 atctaactgt cttttttcac cgttttcagc ataagcatct acgttgtatt taaaacctct
     9661 tccaattact tccccattat ctgctttgat atcagtttct gcaggcattg gagctttgac
     9721 taccgcataa taatctttac ctacataagt agactgatag tagtcccaac ctttccatcc
     9781 tataaaagca atcaatatga taacgatcaa ccctaaaatt tttttcatga aagatctcct
     9841 tttttatttt tatta
//
LOCUS       DAZKMK010000101         9676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000101 DAZKMK010000000
VERSION     DAZKMK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.103"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..195)
                     /locus_tag="ACG8V1_002422"
     CDS             complement(<1..195)
                     /locus_tag="ACG8V1_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330465.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="ACG8V1_002423"
     CDS             complement(211..825)
                     /locus_tag="ACG8V1_002423"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0330466.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="ACG8V1_002424"
     CDS             complement(972..1655)
                     /locus_tag="ACG8V1_002424"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0330467.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="ACG8V1_002425"
     CDS             complement(1764..2222)
                     /locus_tag="ACG8V1_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0330468.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="ACG8V1_002426"
     CDS             complement(2306..4069)
                     /locus_tag="ACG8V1_002426"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0330469.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="ACG8V1_002427"
     CDS             complement(4290..5750)
                     /locus_tag="ACG8V1_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330470.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACG8V1_002428"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACG8V1_002428"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0330471.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="ACG8V1_002429"
     CDS             complement(6907..8334)
                     /locus_tag="ACG8V1_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0330472.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="ACG8V1_002430"
     CDS             complement(8365..9579)
                     /locus_tag="ACG8V1_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0330473.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2864 a         2024 c         1610 g         3178 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagaca
//
LOCUS       DAZKMK010000102         9651 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000102 DAZKMK010000000
VERSION     DAZKMK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.104"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8V1_002431"
     CDS             168..362
                     /locus_tag="ACG8V1_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330474.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8V1_002432"
     CDS             631..930
                     /locus_tag="ACG8V1_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330475.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8V1_002433"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8V1_002433"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0330476.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8V1_002434"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8V1_002434"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0330477.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8V1_002435"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8V1_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0330478.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8V1_002436"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8V1_002436"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0330479.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8V1_002437"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8V1_002437"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0330480.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8V1_002438"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8V1_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0330481.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8V1_002439"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8V1_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0330482.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8V1_002440"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8V1_002440"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0330483.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8V1_002441"
     CDS             7521..8291
                     /locus_tag="ACG8V1_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0330484.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8V1_002442"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8V1_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330485.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         3014 a         1961 c         1505 g         3171 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg t
//
LOCUS       DAZKMK010000103         9595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000103 DAZKMK010000000
VERSION     DAZKMK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.105"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..642)
                     /locus_tag="ACG8V1_002443"
     CDS             complement(<1..642)
                     /locus_tag="ACG8V1_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0330486.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTK"
     gene            complement(644..1408)
                     /locus_tag="ACG8V1_002444"
     CDS             complement(644..1408)
                     /locus_tag="ACG8V1_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0330487.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1416..2093)
                     /locus_tag="ACG8V1_002445"
     CDS             complement(1416..2093)
                     /locus_tag="ACG8V1_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0330488.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2130..3515)
                     /locus_tag="ACG8V1_002446"
     CDS             complement(2130..3515)
                     /locus_tag="ACG8V1_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0330489.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3725..3809)
                     /gene="ffs"
                     /locus_tag="ACG8V1_002447"
     ncRNA           complement(3725..3809)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8V1_002447"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3832..4347)
                     /gene="tadA"
                     /locus_tag="ACG8V1_002448"
     CDS             complement(3832..4347)
                     /gene="tadA"
                     /locus_tag="ACG8V1_002448"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0330490.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4454..5311
                     /locus_tag="ACG8V1_002449"
     CDS             4454..5311
                     /locus_tag="ACG8V1_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330491.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5392..5727)
                     /locus_tag="ACG8V1_002450"
     CDS             complement(5392..5727)
                     /locus_tag="ACG8V1_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330492.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5794..7164)
                     /locus_tag="ACG8V1_002451"
     CDS             complement(5794..7164)
                     /locus_tag="ACG8V1_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0330493.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7242..7778)
                     /locus_tag="ACG8V1_002452"
     CDS             complement(7242..7778)
                     /locus_tag="ACG8V1_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0330494.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8087..9568
                     /locus_tag="ACG8V1_002453"
     CDS             8087..9568
                     /locus_tag="ACG8V1_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330495.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1970 c         1502 g         3215 t
ORIGIN      
        1 ttttgtttgt ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa
       61 taatacaaaa aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat
      121 tggatcagta acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag
      181 gataaaaaag cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat
      241 gccgatttga aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat
      301 aaacaaaata ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat
      361 tttcacttca taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag
      421 gatggttttg aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt
      481 ctcttgatct aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag
      541 tggtcgaaag ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa
      601 caatccagca tattccatcc actgccatct cgaatttttc atattagaag ctccattcat
      661 ccagtgattg tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg
      721 taaaacctga taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg
      781 tttcatagtt atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg
      841 ctttatccat aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg
      901 tctctacaaa agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat
      961 tcttatctgg gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa
     1021 ctactttttc ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt
     1081 cttcccatgt aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat
     1141 agactttttt cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt
     1201 agactgtctc tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt
     1261 ctggtgactt agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt
     1321 cttccacaaa tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta
     1381 aatcaattaa atatccttta tagtccattc atccactact cttccctttt tctgatcgta
     1441 aatgctgttt ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag
     1501 gtttttgctg actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact
     1561 ggtccttttt tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga
     1621 gttgtctttt cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg
     1681 caatattcgt aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga
     1741 ttatccgctt ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata
     1801 taatcatatc gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct
     1861 tcacgataat cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct
     1921 aacagtgtca ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc
     1981 aaagcagctg tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta
     2041 gcaatttctg ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca
     2101 ccacctatct tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa
     2161 tctcctacca cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca
     2221 atcgttggaa aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta
     2281 ttcagatcga ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt
     2341 ccattataga ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag
     2401 ttacgtaaaa agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca
     2461 cccaatgtga catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga
     2521 ttgacccgcc cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc
     2581 gtctttcctc gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag
     2641 atatctagac tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc
     2701 ccacgttcta gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg
     2761 gtagggatgg ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg
     2821 acttcaccga catactgccc gtactttcca gcggctgcta tttggacatc attgattacg
     2881 tgaccttctc tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt
     2941 tcttttgcaa tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc
     3001 acttgtgaac gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc
     3061 cagccattcg gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc
     3121 gtctggtgtt tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta
     3181 tttttatcga ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc
     3241 aactcttctt tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca
     3301 acttcattca tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa
     3361 cgatccacaa aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt
     3421 tctcgtttac gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc
     3481 agatcattcg tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt
     3541 tatcatacca taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct
     3601 gccttgaagc aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg
     3661 aacattcatt tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt
     3721 ttcattataa tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca
     3781 cgcttcacaa gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc
     3841 attcgttgac cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc
     3901 agtcaaaatc atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt
     3961 aaaccgctcg tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata
     4021 cgcgccgaaa taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca
     4081 aggctctaag gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca
     4141 ggcttccttg attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag
     4201 gttatatcct cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc
     4261 atgaagcatt tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc
     4321 tactgcatca agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt
     4381 actttagcat atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca
     4441 agaggtgaac aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa
     4501 aaactctcca gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg
     4561 gatcaaacaa gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt
     4621 aatcaacgat caaaaaaaac acccatggta tcgctatctt tttaaagatg cttcttgtgt
     4681 tcaagaaaaa agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa
     4741 aaaagaatgg caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta
     4801 tacagacaca gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga
     4861 aatcaacggt atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt
     4921 tggttctttc cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat
     4981 ttttttaaca gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt
     5041 ggctttacat tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat
     5101 agaactgatc aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg
     5161 caatgtcagc tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat
     5221 cgaaaaattt caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg
     5281 ctatctttta ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat
     5341 tttgtcacaa ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat
     5401 tttttcaaca aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc
     5461 accataaaca gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga
     5521 atccaagtca ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa
     5581 tttgtcatag acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat
     5641 tacttttccc gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc
     5701 gattgttact tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat
     5761 agcatagaca aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa
     5821 aaagttcttt actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac
     5881 tagcaaaacc catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg
     5941 ccattccttt tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc
     6001 taatgcatag ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg
     6061 tacgactttt aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt
     6121 gaacactaaa agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag
     6181 tttttcctgc tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg
     6241 catctgattt tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt
     6301 atgaggccat atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc
     6361 tgaactaacg gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag
     6421 ccagctaatg atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg
     6481 tcctttattt tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca
     6541 gtatagcgga gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc
     6601 ttctcctgaa caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac
     6661 aagttctgcg ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt
     6721 ttctgataaa atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc
     6781 cttcatttga gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac
     6841 tttttcaccg ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct
     6901 atattcatac ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc
     6961 attgctgtag ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc
     7021 tgccggtttt agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga
     7081 aaaaaaatcg gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg
     7141 taacgttgtc gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta
     7201 cgaaaaataa aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt
     7261 aagatatttt ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt
     7321 tcaagaaaat cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc
     7381 aattcaagcg acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc
     7441 cgcttaaata gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca
     7501 tggatagaaa aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat
     7561 tcagctaagt agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg
     7621 ctgattgatt tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac
     7681 tctcttgcag taattgactt cattccatat tcttccacgt ataccatact tttgaacagc
     7741 aaatattctt tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat
     7801 ctacccataa ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat
     7861 atttagttat atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa
     7921 aataccctag gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat
     7981 ataaaaaaga aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca
     8041 gtttttgtct aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc
     8101 aagaaaaaaa gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca
     8161 agaattatcc aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat
     8221 aaatgtatca ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg
     8281 agagatccaa accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa
     8341 attatgctac ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc
     8401 acaaactgct gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct
     8461 cgaaaaactg acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc
     8521 tgtaaaaaat tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt
     8581 ttttctttca gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc
     8641 actaaaaatg aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat
     8701 ctacttgctg ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac
     8761 ttcttctgaa atcactgaat tgatggactt attgcagcat acacatgatt attcagaata
     8821 ctttacttct ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt
     8881 taattatttt ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat
     8941 tggtttttta ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac
     9001 ttacttaact caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta
     9061 ttttcttgtg ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc
     9121 tacactttgc tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa
     9181 attaagagaa aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca
     9241 ataccttagt caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat
     9301 ccatttgcag ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca
     9361 gttatttcgc aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat
     9421 tacagatcag catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca
     9481 caatacagtg ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg
     9541 ttatgagaac aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tattc
//
LOCUS       DAZKMK010000104         9311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000104 DAZKMK010000000
VERSION     DAZKMK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.106"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..710)
                     /locus_tag="ACG8V1_002454"
     CDS             complement(42..710)
                     /locus_tag="ACG8V1_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0330496.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            844..1131
                     /locus_tag="ACG8V1_002455"
     CDS             844..1131
                     /locus_tag="ACG8V1_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330497.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1547..3085
                     /locus_tag="ACG8V1_002456"
     CDS             1547..3085
                     /locus_tag="ACG8V1_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0330498.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3082..4047
                     /locus_tag="ACG8V1_002457"
     CDS             3082..4047
                     /locus_tag="ACG8V1_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330499.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4034..5356
                     /locus_tag="ACG8V1_002458"
     CDS             4034..5356
                     /locus_tag="ACG8V1_002458"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0330500.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5419..5523
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5597..6340
                     /locus_tag="ACG8V1_002459"
     CDS             5597..6340
                     /locus_tag="ACG8V1_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0330501.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6355..7347
                     /locus_tag="ACG8V1_002460"
     CDS             6355..7347
                     /locus_tag="ACG8V1_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0330502.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7359..8096
                     /locus_tag="ACG8V1_002461"
     CDS             7359..8096
                     /locus_tag="ACG8V1_002461"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330503.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8086..8808
                     /locus_tag="ACG8V1_002462"
     CDS             8086..8808
                     /locus_tag="ACG8V1_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0330504.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8892..>9311)
                     /locus_tag="ACG8V1_002463"
     CDS             complement(8892..>9311)
                     /locus_tag="ACG8V1_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0330505.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3050 a         1604 c         1967 g         2690 t
ORIGIN      
        1 tgatatttct gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca
       61 agaggatcaa gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca
      121 gatccaattt ccagatactt cactttattc aatgtataag atagaataga acagctaagt
      181 aatgtataca gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa
      241 gtaatcaaga aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa
      301 atgatatctg ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca
      361 gaaagaatac ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga
      421 aggctctccc ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga
      481 aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt
      541 gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc
      601 ccaccatctg ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct
      661 gcgcctaaaa tgattccgca caattctgta aataaattta attttttcat ttctagcctc
      721 ttttcttttg aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag
      781 aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga
      841 aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat
      901 caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat
      961 aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac
     1021 atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa
     1081 ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata
     1141 agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg
     1201 tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt
     1261 tctactttat tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata
     1321 gctgattcca agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga
     1381 accaaaaagt tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt
     1441 atttttttct tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa
     1501 aggaaagaaa tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat
     1561 acaaacatta agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat
     1621 ttctctcatt tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc
     1681 caatcataaa aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc
     1741 ttcacttgcc ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt
     1801 gattgcactt gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc
     1861 tgaaattgac ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg
     1921 cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc
     1981 cttagtttta atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg
     2041 gacatttatt ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat
     2101 cagttctgcc ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa
     2161 acctcgatat tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt
     2221 atatcagtct gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga
     2281 acctgaaatc ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt
     2341 tgaattaaaa ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca
     2401 aatcgatatc tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga
     2461 agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt
     2521 gaataagcaa ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt
     2581 ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc
     2641 tatctctgat cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata
     2701 taaaggaatc caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag
     2761 tttgcgttac caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga
     2821 tagccagctc aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc
     2881 ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa
     2941 agcactgaat aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca
     3001 agtcaatgtc gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg
     3061 attagtaaag gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag
     3121 tatgatggaa agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt
     3181 gttttggttg gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta
     3241 tctaaaccat cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg
     3301 cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg
     3361 acagtcaagc aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga
     3421 gcagaccgtg tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat
     3481 cgtatgccta gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt
     3541 gctgcaagcc cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca
     3601 agaaactcat tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt
     3661 ttcgttacgc acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag
     3721 gatggagaaa ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac
     3781 tacgttcggc atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta
     3841 gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt
     3901 gatacgccta tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta
     3961 gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga
     4021 gaggaggaag gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt
     4081 aagtgctgct tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct
     4141 atggggcggg acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt
     4201 agaggcacgt aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc
     4261 caactgggaa gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg
     4321 aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga
     4381 tgattcatct attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac
     4441 cgggcaaaga cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga
     4501 ctttttatca ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc
     4561 ttttgagcta gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa
     4621 tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga
     4681 agcatctggc attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac
     4741 tcgctatcgt ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac
     4801 tgggagacag ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa
     4861 acacggaatt gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg
     4921 agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta
     4981 tgtagctaag cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac
     5041 gatcgtctac gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc
     5101 gtcagatcaa gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga
     5161 accagttgct aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt
     5221 gattagcgag caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt
     5281 gactaaaaaa gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc
     5341 ctatttattg aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc
     5401 tacactttaa aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt
     5461 gtgaacttag acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac
     5521 gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa
     5581 gaaaggaggt aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt
     5641 aattatttgg cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc
     5701 tgctgctgta ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac
     5761 gacattaaat gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc
     5821 cttggcaatg gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc
     5881 acaaatgttg ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat
     5941 gcttccaaaa gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc
     6001 ggatacattg aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga
     6061 tacagggaag atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg
     6121 tttaaaagtg gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc
     6181 tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt
     6241 ttttgctgcg atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt
     6301 attgaaaaaa gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa
     6361 aaattatggc ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa
     6421 acaagtagca aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta
     6481 ccgaatacta atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct
     6541 ggtttaaatg tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt
     6601 gcggacaaag cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat
     6661 ggacagaata taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc
     6721 gccaccctat cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca
     6781 ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg
     6841 gacttttcaa aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa
     6901 tcctcagata tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt
     6961 attgaactaa actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg
     7021 gtaattatta cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg
     7081 gatgccacga aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg
     7141 ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg
     7201 tcaaaaaatt atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc
     7261 aattatacag cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa
     7321 ctattcacta atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact
     7381 tgaatgtagt cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag
     7441 atacctttac atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt
     7501 taacaggagt ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg
     7561 gtctccatac agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg
     7621 tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag
     7681 taagggatta tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat
     7741 tatctggcgg gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt
     7801 acttgcttct tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag
     7861 actggatgtt gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata
     7921 tcgaagaagc aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg
     7981 tgattgctga gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat
     8041 cagagttgaa gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct
     8101 cattgtcatt tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg
     8161 acgatcatca attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt
     8221 aagactcaag ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa
     8281 caagttgagc cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt
     8341 tggacgaatg taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca
     8401 gccataaatg agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat
     8461 tatatccaag cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa
     8521 caagactata ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca
     8581 gcagtctggc aactagctaa agcagtccca atcgaccact tacttattga aacggatgga
     8641 ctacatgcag cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg
     8701 cttgaagaac agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa
     8761 aaaaatactt atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg
     8821 acacaactag tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta
     8881 ataaatgaat cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa
     8941 ccataacgtg aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca
     9001 ccatttaagt aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg
     9061 taagaaccgc ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga
     9121 gtagcagcag tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt
     9181 ttttccgttt gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga
     9241 acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt
     9301 tgtcctgagt a
//
LOCUS       DAZKMK010000105         8942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000105 DAZKMK010000000
VERSION     DAZKMK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.107"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACG8V1_002464"
     CDS             <1..260
                     /locus_tag="ACG8V1_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330506.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ACG8V1_002465"
                     /pseudo
     CDS             467..667
                     /locus_tag="ACG8V1_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="ACG8V1_002466"
     CDS             complement(1379..1984)
                     /locus_tag="ACG8V1_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0330507.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="ACG8V1_002467"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="ACG8V1_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2881
                     /locus_tag="ACG8V1_002468"
                     /pseudo
     CDS             2666..2881
                     /locus_tag="ACG8V1_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3019..3990
                     /locus_tag="ACG8V1_002469"
     CDS             3019..3990
                     /locus_tag="ACG8V1_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0330508.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="ACG8V1_002470"
     CDS             4130..5443
                     /locus_tag="ACG8V1_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0330509.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="ACG8V1_002471"
     CDS             5486..6352
                     /locus_tag="ACG8V1_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0330510.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="ACG8V1_002472"
     CDS             6349..7197
                     /locus_tag="ACG8V1_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0330511.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="ACG8V1_002473"
     CDS             7211..8683
                     /locus_tag="ACG8V1_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0330512.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="ACG8V1_002474"
     CDS             8700..>8942
                     /locus_tag="ACG8V1_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0330513.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DAZKMK010000106         8777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000106 DAZKMK010000000
VERSION     DAZKMK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.108"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(375..2756)
                     /locus_tag="ACG8V1_002475"
     CDS             complement(375..2756)
                     /locus_tag="ACG8V1_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0330514.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2746..3393)
                     /gene="recU"
                     /locus_tag="ACG8V1_002476"
     CDS             complement(2746..3393)
                     /gene="recU"
                     /locus_tag="ACG8V1_002476"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0330515.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3457..3996
                     /locus_tag="ACG8V1_002477"
     CDS             3457..3996
                     /locus_tag="ACG8V1_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0330516.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4086..4505
                     /gene="gpsB"
                     /locus_tag="ACG8V1_002478"
     CDS             4086..4505
                     /gene="gpsB"
                     /locus_tag="ACG8V1_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0330517.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4527..4894
                     /gene="rnpB"
                     /locus_tag="ACG8V1_002479"
     ncRNA           4527..4894
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8V1_002479"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5103..6269
                     /locus_tag="ACG8V1_002480"
     CDS             5103..6269
                     /locus_tag="ACG8V1_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0330518.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6315..7811
                     /locus_tag="ACG8V1_002481"
     CDS             6315..7811
                     /locus_tag="ACG8V1_002481"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0330519.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7892..8575
                     /locus_tag="ACG8V1_002482"
     CDS             7892..8575
                     /locus_tag="ACG8V1_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0330520.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8584..>8777
                     /locus_tag="ACG8V1_002483"
     CDS             8584..>8777
                     /locus_tag="ACG8V1_002483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330521.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2773 a         1586 c         1851 g         2567 t
ORIGIN      
        1 cagacaaatt atgataatta agcatactat cagataactc aaaatgaagc cttctttcaa
       61 aaaattattc aaaaccatat attgtatttt ttatttgata ataacaatgg gattattaag
      121 aagtggcact aaataaaaga ctagtgtgta tttagccgat ttaattataa acaagtaaat
      181 ggataaagta tatgtaatga taaaagaaaa atggattttc taatttttaa gctaaatgtt
      241 agatcacaca tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca
      301 tgatttttat ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg
      361 tggtttcttt tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca
      421 tttgcagcat ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa
      481 gacggtgtcg agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg
      541 cttgtttctg ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta
      601 gagctagagc tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg
      661 gaagatggaa tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg
      721 tagtacagtt caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc
      781 acgttagctg aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat
      841 tctgaagtga caggcgtcat ttttttattg taacctgtcc acacggaaat cgaataattt
      901 ggcgtatagc ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca
      961 tactcgtcat ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt
     1021 tgcgcattcg taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa
     1081 gctgtttctg gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct
     1141 tggaagacga ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc
     1201 gcataggctg ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc
     1261 gtattactag agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt
     1321 aagaaagaag ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta
     1381 cgggataagt aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt
     1441 cctacaggag tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta
     1501 gaatatttca aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca
     1561 aaatcacggg aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga
     1621 cgtccgccaa tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact
     1681 tgcatctcgt cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc
     1741 ttttgggcgt ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta
     1801 tctgtttttt cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta
     1861 ttgtcatcgg attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga
     1921 tcatattctg tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt
     1981 cttttcttcg cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc
     2041 ccagcaagca atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc
     2101 tcagaagctg tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag
     2161 gtcaagatct cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg
     2221 gccttgcgtt tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat
     2281 tgctgtgtca aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa
     2341 ccgatgatcc gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac
     2401 acgatcgcat cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct
     2461 gcgcctaaat cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg
     2521 tctaattttt tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca
     2581 cctgctaaaa agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat
     2641 ccttttttat gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc
     2701 tttttaggag taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt
     2761 gctttctcct tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat
     2821 ccgcggagaa ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag
     2881 tgatttgcgt cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa
     2941 ataaaaacag cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc
     3001 gaggcatgct ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt
     3061 tttgttcttg gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata
     3121 gtctgtagtt gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg
     3181 atagtctact ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg
     3241 ggaaagataa tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc
     3301 gaattcagtg atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata
     3361 ataaggttgg ccatttggat agcgcaatac catctttaat cacactccta ggccacatta
     3421 tagcaaaaaa gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat
     3481 gtgtctgggt atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa
     3541 gttgttaaaa atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg
     3601 gtacttgtca gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg
     3661 aaagaagaat atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat
     3721 aactggaatg aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc
     3781 gaagctgtta gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt
     3841 atacttgagc atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg
     3901 aagtattttt ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg
     3961 atcagtatgg acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa
     4021 cttttttcct atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg
     4081 taaatatggc gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa
     4141 aaatgcgcgg ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt
     4201 atgaaactta cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa
     4261 aagtagctca attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg
     4321 caccaaagag cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac
     4381 gtgaagtatt tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta
     4441 atccaaataa ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat
     4501 tttaattttt gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc
     4561 tgaggaaagt ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc
     4621 ataagctaag gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct
     4681 taagtatcct tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg
     4741 cggtaaaccc ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa
     4801 gaacaggaca aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc
     4861 tggaaaagca aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc
     4921 gtgacaaaag taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac
     4981 ttcagatgaa tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg
     5041 agtaaaagtg tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt
     5101 gtatggaaaa aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac
     5161 tggttggtaa agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac
     5221 gttttgaagg aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc
     5281 gagtaaaaat cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg
     5341 taaaagcatt gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa
     5401 aatcgatcaa gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa
     5461 tcgttgaacg tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg
     5521 gcgcacattt tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata
     5581 cgacaggacc aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga
     5641 aagaaaacat ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt
     5701 atgatcctgt atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata
     5761 tcgcaccagg attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa
     5821 tcatggaaaa tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata
     5881 ttactggttc agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa
     5941 tcggattagg ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata
     6001 aagaatacgg ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa
     6061 ctgttcggaa attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta
     6121 aatatatctt gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa
     6181 aaagaaaatt gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc
     6241 cgcctagaaa aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt
     6301 tggaggaata gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc
     6361 tacaacaagc tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta
     6421 gtgcataccg aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg
     6481 gtccaaaaat caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag
     6541 ttggaactgc cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg
     6601 aagatcttat gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag
     6661 ctagagaaac acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa
     6721 caaaacagtt gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga
     6781 atcaatacga acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag
     6841 aaggaattct ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact
     6901 ttttaaatcg gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac
     6961 aacttggtta tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg
     7021 gaattaatcc gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg
     7081 ctgtttttgg agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata
     7141 aatatgcgta tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt
     7201 tgtttaacga gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct
     7261 ttcaagaatg tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat
     7321 tgaaccatac aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc
     7381 atatcatcat tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg
     7441 aactacctga aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg
     7501 atgtggaagg catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct
     7561 cttatgcatt agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag
     7621 atgtggaaaa tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc
     7681 atatccatca atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg
     7741 aaccgcttaa tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc
     7801 aggtaaaata aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt
     7861 cagctattta cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta
     7921 tagtatggac caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt
     7981 tgtcaagtta gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct
     8041 tgcagcagct tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc
     8101 aaagcaacag cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat
     8161 gattttttcc attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact
     8221 atctctggaa ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg
     8281 cattttgcgt actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact
     8341 gtatccgcaa acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca
     8401 tttttatgaa aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga
     8461 agccaaacga agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc
     8521 tattgacgat gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag
     8581 gttgtgacag aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc
     8641 tggaacactg tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca
     8701 atttcaaaaa atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca
     8761 ggagaacacc gtttatt
//
LOCUS       DAZKMK010000107         8767 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000107 DAZKMK010000000
VERSION     DAZKMK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.109"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8V1_002484"
     CDS             226..894
                     /locus_tag="ACG8V1_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0330522.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG8V1_002485"
     CDS             complement(1094..1414)
                     /locus_tag="ACG8V1_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330523.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG8V1_002486"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8V1_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0330524.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIETVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8V1_002487"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8V1_002487"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0330525.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8V1_002488"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8V1_002488"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0330526.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG8V1_002489"
     CDS             complement(5230..6684)
                     /locus_tag="ACG8V1_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0330527.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACG8V1_002490"
     CDS             6859..7764
                     /locus_tag="ACG8V1_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0330528.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8V1_002491"
     CDS             7855..8532
                     /locus_tag="ACG8V1_002491"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0330529.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8V1_002492"
     CDS             8529..8699
                     /locus_tag="ACG8V1_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330530.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
BASE COUNT         2679 a         1710 c         1718 g         2660 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacgg tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatga
//
LOCUS       DAZKMK010000108         8591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000108 DAZKMK010000000
VERSION     DAZKMK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.110"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..1516)
                     /locus_tag="ACG8V1_002493"
     CDS             complement(50..1516)
                     /locus_tag="ACG8V1_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0330531.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1903..2499
                     /locus_tag="ACG8V1_002494"
     CDS             1903..2499
                     /locus_tag="ACG8V1_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0330532.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2557..3039)
                     /locus_tag="ACG8V1_002495"
     CDS             complement(2557..3039)
                     /locus_tag="ACG8V1_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330533.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3145..3342)
                     /locus_tag="ACG8V1_002496"
     CDS             complement(3145..3342)
                     /locus_tag="ACG8V1_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330534.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3637..4395
                     /locus_tag="ACG8V1_002497"
     CDS             3637..4395
                     /locus_tag="ACG8V1_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0330535.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4455..4883
                     /locus_tag="ACG8V1_002498"
     CDS             4455..4883
                     /locus_tag="ACG8V1_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330536.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4929..5001
                     /locus_tag="ACG8V1_002499"
     tRNA            4929..5001
                     /locus_tag="ACG8V1_002499"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4962..4964,aa:Gln,seq:ctg)
     gene            complement(5151..5522)
                     /locus_tag="ACG8V1_002500"
     CDS             complement(5151..5522)
                     /locus_tag="ACG8V1_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0330537.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5737..5922
                     /locus_tag="ACG8V1_002501"
     CDS             5737..5922
                     /locus_tag="ACG8V1_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330538.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6005..6193)
                     /locus_tag="ACG8V1_002502"
     CDS             complement(6005..6193)
                     /locus_tag="ACG8V1_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330539.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6217..6480)
                     /locus_tag="ACG8V1_002503"
     CDS             complement(6217..6480)
                     /locus_tag="ACG8V1_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330540.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6638..6895
                     /locus_tag="ACG8V1_002504"
     CDS             6638..6895
                     /locus_tag="ACG8V1_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0330541.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6966..7157)
                     /locus_tag="ACG8V1_002505"
     CDS             complement(6966..7157)
                     /locus_tag="ACG8V1_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330542.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7298..7618)
                     /locus_tag="ACG8V1_002506"
     CDS             complement(7298..7618)
                     /locus_tag="ACG8V1_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330543.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7641..8075)
                     /locus_tag="ACG8V1_002507"
     CDS             complement(7641..8075)
                     /locus_tag="ACG8V1_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0330544.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8410..>8591
                     /locus_tag="ACG8V1_002508"
     CDS             8410..>8591
                     /locus_tag="ACG8V1_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0330545.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2723 a         1695 c         1361 g         2812 t
ORIGIN      
        1 aaaacaggaa ctgctgcaaa taagcaattc ctgtttttat gttcattgat taatgaatag
       61 agcccattag ttttttgatt ggttctttca cagtaaatag caagatacca ccgacgattg
      121 ctactgcacc gataataccg aagtaagcag attctgttcc cggtgtgtag aagcgtgcaa
      181 tttgtgcatt gattgcttgt gaagaagcat cagctaagaa ccaaatagcg attgtttgtg
      241 attcaaatgc tcgtggtgct aattttgttg tcacagacaa accaactggt gaaagacaca
      301 attcagcaat aaccacgatg aagaaacttc ccaacaacca caatggactt acttttccac
      361 ttgtaccata aagcataccc ggcaacatca atagcaagta cgataatcct gcaaataaaa
      421 gtccaagaga gaatttcact actgtagatg gttgacgttc accaagtttc gtccacatcg
      481 ttacgaatac tggtgtcaga ataaagatga aaattggatt caatgattga taccagcttg
      541 cttggatcgt tacaccaaat agcgtagatt gtgtacgttc actagcaaat aaagctaaga
      601 tagatgaacc ttgttcttgt aatgaccaga aaatgattgc tgcaaggaat aatgggatat
      661 aagcccatac tcttggtttt tcttcagcag ttacatcttt tgaagtcagc attttgataa
      721 agtaatacaa cggtaagata atccccaata cactgattgt attgacaaaa aagtcaattg
      781 tcagatgacc agttagttgt gccccgccga atactgcagc gataacaaca agtgcgatga
      841 tcaagttacg gatgaatcct ttacgttctt cagttgtcaa aggattcggc ggtgttgtac
      901 ccactccgtc taatgtcttg cgtccttgga tgatgtattg taataatcca aagaacatcc
      961 caatagcagc taaagagaac cctaaatgat agttatattc ttgaccgatt gtaccaacga
     1021 ttagtggtgc taataatgcc cctaagttga cacccatgta aaaaatcgaa aatcctgcat
     1081 ctcttcgcaa gtcagtcttt gaataaagat gtccgaccat gccagaaaca tttggtttca
     1141 aaaaggcggt tccgataata atcaatgcca tcgaaacaaa caatgtactt acaccaaatg
     1201 gtaaagccaa gacgatgtgg ccaagcataa tgaaaactcc accgaagaat acagtgcgtt
     1261 ttgctcctaa cactcggtca gatacccatc cgccgacgat actagacatg tagatcaaag
     1321 atccgtagat cgacatgata gctaatgctg ttgctcgtgg taatccaagt cccccgttgg
     1381 caactgtatc gtacatataa tagataagta atgcacgcat accgtaataa ctaaatcgtt
     1441 cccacatctc agtgaagaac aaagttgaca atcccttcgg ctgcccgaag aagcccttgt
     1501 cttgctgttt ttccataatc tataccccaa ttctttaaat tttcagaaaa tattatacat
     1561 ctttaccgaa aaatttaact aaaaaactta tttaagaaat ataaactaat tttctataaa
     1621 agcaagtaat ttttaatggc aatatcataa aaaaaagaag atagagattt aataattata
     1681 tatcagctgt ttttttcaaa taagcaaaaa ttcctaaaac acaacaatga aagcgataac
     1741 taagaaaatc cttacataac gatttttctt attaaaattg ttttttgaac ttcgcttatt
     1801 ttttgtgagc atatctgttt acttactttt tgtaaggtgc tatacttcat ttcgtaatca
     1861 attaaaaaaa tgcaaaacgc aaatcaaagg agaaatttta ttatgacaac ttttactgac
     1921 actttgaaaa accgccgttc aatctatcat ctaggacgta atgtttcact ttctaatgaa
     1981 gaattaacaa ctttgatcaa agaagcaatt aaagaaagcc caactgcttt caatgctcaa
     2041 tcaacacgcg cagttatcct tttcggagat gcacatgaaa aactttggga aatcactgaa
     2101 gaagcacttc gcccattgac tccagcagaa gctttcccaa atacacaaaa caaattagct
     2161 ggtttcaaaa atggttatgg tacagtatta ttcttcaaag atactgacgt ggtaaaaggt
     2221 ctacaagaac aatttgaatt atatgcagac aacttcccag attggtcaga acaatcaaac
     2281 ggaatcgcaa cagctaatac ttgggtagca ttagttgata aaggattggg cgcaaacttg
     2341 caacattaca acccagttat tgacgaagca gttgcaaaag aatggaatat cccttcaaac
     2401 tggaaactac gttcgcaatt agtattcggt tcaccagaaa caccggctga ggaaaaagaa
     2461 tacatgaacg atgccgatcg cttccgcgta tttggataat tttatttgaa ataaaaaaca
     2521 gaggttgtga caaacatctt gtcgcaacct ctgtttttat tctggatcaa tcataatatc
     2581 aatctctgcc tcatcgttta agcgttggga caaaaatgga actaattctt ctaatgatgc
     2641 agaaaatttc agcatcggtc ctcgccatcc gtttcggaaa aaattcattt ttacgatttt
     2701 agaacctttt ggctgatata atacaatttt attgatctca gaaaaaggaa cacctttttt
     2761 gtcaaagcta ttcaaaacaa gtccttcttc tgtgattcct ctactgtcca acaaaaaact
     2821 aataaaaatc attgctgaca agatcccttt tgccaattca tctgctgtag gatgactgat
     2881 gagtgtatac caaatgacta gtgacactaa gaagggtatc accaagtaga cccatcgatt
     2941 tcgcacagtc cgataaataa tttcttttcg aaccagccaa agaaataaaa acatcacaac
     3001 aacgaataaa aacaatagaa ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc
     3061 ttctccagaa aataggaaaa gaatactgat ttaccttctc aaagaaattg gacttgcagc
     3121 acgccgcacg tccaattgtt ttctttattc aaatgtatca tattttgcaa caaccatgct
     3181 tgctttttca tctaataatt cttggtcctc tgcagcacat tgggcaactt tgaccacggc
     3241 gcagtttgtc atttttgata caacttcacc aatgaccgtt tttgtaaaac caattggttt
     3301 gcatgcatat tgttctccta aattaatcat ttcttccgtc atcaaaatcc tcctaaactg
     3361 ctggaacttt tctccacatt accactgcta tttctttgga gatttaactc attgattttt
     3421 atttacagtt aaaaatcaac cttacaggca ttatagcgta ctgttttcgt tttcacaatt
     3481 aaaacagctg ttgtatttag aagaattttt ttcaaaaaat taaaacatat tccttaaaaa
     3541 ctaattattt cctacatatt aaaatataat acttccattt ccaataaaaa tacatttata
     3601 ataggaaaga taaacagctg aaaagggaga tttcaaatga agaaaaaaac gttcaaaaaa
     3661 ctattgtttc tagggggcac agctgctctt ttattaagcg gtacaagtta ttacctacat
     3721 atccattctc cagaacatac aatgaacact gcacaaaaaa gttcaacaca aaaaacgaaa
     3781 aagacaaaag agaaagcaga agttttacta gatgttccat tagagagcca gtttgattct
     3841 ccttctttag aaaatggctg tgaagtcact tcgctttcta tgcttttatc tttttatggt
     3901 tatgaaacaa ctaaaaacca gttagctgag caattggatt acgtccctgt tttcaatgca
     3961 gatgggactc atggcgatcc taatgaagga tttgttggtg atatcagtgg cggagactgg
     4021 gctatggtcg tctatgttcc cccagttgcc tcccttgctc agcaaatcgt ccaaacggat
     4081 tatcacactt ttccaaaaac cgatgctcaa ctcgatgata tcaaaaaagc actatctaaa
     4141 ggaaatcctg tctggacttc tgtgactatc aattttgaag tcccagatga aactgatttt
     4201 atgacttgga ccacaaataa tggagaagta agagtcactc ctcttgttca tgcttgtgta
     4261 gtcactggat atgacaaaga aaatatttat gtcaacgatc cctatggtgt aaaaaatcga
     4321 gcagtaccga tcaacgattt ttcggcgatt ttcactgcaa tgggtggaca gatgctaact
     4381 ttggagaaaa gctagtaatt caaacaaaaa tcccttatac tcaagatacc aaacctttga
     4441 agggagcgta gataatggaa aaattcacac ataaaaaaat ggatccaaat gaaattccga
     4501 ttatttttgt ccgtgactgc aagggaaatg ttcaaggtaa agtctctatt aatgagtgga
     4561 acgagcgtcg cagacctgct accctcaatg aactggaaat caaattgtat cgtcaatctc
     4621 ttgtttatta cgcagatcaa gagtacgaga aagccaccga tttattgaaa tttctaattg
     4681 ctcgaacaga gtatactcgc tttgaatata ttgaacgttt agcaaacatc tatcatatta
     4741 tgaatgaacc tgtcaaagaa tatcagttat tggacaccgt cttgtcagta gcagaactga
     4801 ttgcccttcc tgctggatta gaaaagaaac tagtacgccg ccttttacga gtaaagcaac
     4861 aactatccga tcaagaaaaa taacttgcgt ttttttagga aagtgctatg attattttgt
     4921 tgaataaatg ggatgtagcc aaactggtaa ggcacttgac tctgaatcaa gcaattgtag
     4981 gttcgaaccc tgccatccca atttgatgtg cttgctgaaa aaatgaaccc cctgtaaaag
     5041 aacaatcttt ttgcaggggg attattgtat tatttttact acttgtattt agtttacata
     5101 attaaattat gaataataaa atcgggcgta ttcttaatac aaaaactgat ttacccaata
     5161 attgctaatg ctttctccgg aactttttct ctaggaactt cctcatacgt ttctatgtgt
     5221 gctcctttat tgctaatttt caaataatga ttttctttta acacagaaag accattaaat
     5281 tcaaccgttc gttctttccc attggcatca gcagctttct gcacatacct tgctgtacca
     5341 tattcattta cttcctctgg tttttgtgtc tttacataga cctctccttt ttcaactaat
     5401 ggatttagct gatcaagtat accagggatt tctccataac ttccttcagt ataatatttc
     5461 agtccaaata gcaaaatacc actaatcgcc gccagtccaa tcaaatattt aatcactttc
     5521 atctttctac ctccttgtta tatattcagt atatgggtca gttaaagaaa ttccatccgt
     5581 cccaagttgg agatttttct cctactttag aagcgagaat cgagtgtttt tttagtaaaa
     5641 atgatcgtta tttgatatta ttgacgtgtg gataaatcag tttttctcgt tttgtccatg
     5701 taaaaaatca tgttgatagc aaaggatgtg ttcaaaatga atcaagaaga attatttcaa
     5761 aaagtcaagg aaatgattaa gaacggcaat ttcgatggcg caaaacgatt tatcgaagaa
     5821 cataaagaac aattaggacc ttataaagag aaagcccaaa atcttttaaa agatgtaaat
     5881 atcgacagtg tcaaaaacaa atttaaaaat ctattcaaat aagtaaaact ttcacttttg
     5941 taaaatcaaa ggaggacaat gctgctgcat cgtcctcctt tggttctttt ctgaatactt
     6001 catactaaaa taaacgtgtc ggatcgtaat gataagtcac attgccgttc agtgaggctt
     6061 ttcccaccac atatttacga ttctccaact tcagccacgc ttctcgaaaa gccattaatt
     6121 cttctctttt tacatcaatc ttttcgattt ttccatcgat caaatcttga ataagttgtt
     6181 catatggatt catttttttc ctccatctgc tgtcaatcaa taagttgtaa aaataggttc
     6241 atatgctttt ttggtatcta gatcttctct gactttgact gtcgtataaa taattttttc
     6301 tactgggata ctataataac ttccttcacc attattgaat cggtgaacgt ctttctttgc
     6361 taaaatacca tttattttat tcgtcagtac acgttgttcg acactgttac ttacaacata
     6421 gtaaatcacc cgaatattat tgatcaaatg caaatcgatt tctaacacta catgatccat
     6481 gaaaatccca ctccttcttt cactttgatt atagcaagtt tctcctctaa tttcaaaatc
     6541 gatacagatt ttaccggata aatccacaaa actgcttgcg ccatttcgca caaagtgtta
     6601 ttttgtatca tggactttat ttatcggagg caatagtatg atcgcgatcg gtcaatttgt
     6661 tttttatatt ccttttttta ttatgataag cattcttttc tattacataa aatggacaaa
     6721 aaagaagttt tcagttttac tggcctcttt acctgctgta tattttactt atcaaatttt
     6781 ttcttttagg cattgggaaa caacgtctgt attaataaca cacatcatcg aattgacgct
     6841 ttcagtaatt tttcttatta tttggatcta ttttttatat aagaatcaga attgaatccc
     6901 tcgcatgttt aaaaagaaaa cgattaaaga tgcagcaaat ttctgccgat ctttaatcgt
     6961 tttttctatt ctgttatacc aaaaaaatgt ttgaatcggt gaaggatcgt tccttgtttt
     7021 aattctataa attcttgtgc gatcgggtac ttttcttcca aattttcttt gttcttttcg
     7081 atcaggcttt tcgcttcttc atatttttta tctgcacata gtgcttttac ttcctgtaat
     7141 acttttcctg cttccatttg cctcaccctc ttgccatttt attgctttaa gttttattta
     7201 agttatacgc attattctag tttcatccca aataaccttt tttcattttt actcctcgaa
     7261 ctaatcagtt ttctaaaggc tgattggttc gtctttttta tttttcctcc atttcattct
     7321 gctcttctgt taatttatct aatgctttga ttgatgctgc ggctcttgca taatcatagc
     7381 tgtataattc cataaattta tcaggcagaa caaaccacgc tggatgataa ccgttagctg
     7441 tgacagggaa tcctttatct tgttggttat ctccatccca tctcagagca acaccttttt
     7501 ttcctttcca ctttactaaa gcgatcgagt atcccttctt tgtttgtgtg ccgtcttcaa
     7561 taattgctaa aatttctatt ttttcttttg gcgcacggac ttcttgagga cgaaccattt
     7621 tctattcctc ccttatctca ttacttcacg accaacacac tgcaaggggc atgtgatacg
     7681 acataagaag cggtcgatcc aactaatgct tgttgtatag caccttttcc tgtcgctccc
     7741 ataacgatca aatcaatatt ttcttgtcca gggatgacat tggcaatcaa atttcttgga
     7801 tctccagttt caactattgc gtggatctca gcaatgccgt actctttggc atcattgatt
     7861 tttttcagca tggctacttc tgttctttgt ttttcatctg catagatttt tgaaaaggca
     7921 taggcactat gggtcagctc aactttgttg atgattgaca cgataaataa gtgggcttca
     7981 ttgtcctttg ctaagtcaag ggcttcagcg aacgctttga tcgattgtct tgatccgtca
     8041 actgctacca tgatgttctg gtatctttta ctcattggaa ccgcctcctt tttcttttat
     8101 tgtaacatct caagcaaaaa tacaaaaaat atacgcaata tgtaaataaa atgatgaaca
     8161 aaagcaattt ctttcaagtt atttatacat ttcttttctc atcagaacac ctgttctaat
     8221 cccattattt ctgcattatg tgaaattcct catcattttt cacatcgagc gaacatcatt
     8281 ctgttacaaa aaacgtggat ttgtcataaa ttgtcatgct tcttttacct atgaaatatt
     8341 ttcacgtgtt aaagtattcc tcgttgaaca caacaaataa aactttttaa cgaataaagg
     8401 agatttttca tgacatcact taaaacttta ctttttggaa caactttggc tgccggcgct
     8461 gcattcttca tgggaacgac tgctcatgcg gacgaagctt ataccgttca atcaggtgat
     8521 actctatcaa cgatttctca aaaatacgtt ggtgataact ccttgatcaa tgccattgca
     8581 gaatcaaatt c
//
LOCUS       DAZKMK010000109         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000109 DAZKMK010000000
VERSION     DAZKMK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.111"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8V1_002509"
     CDS             complement(<1..149)
                     /locus_tag="ACG8V1_002509"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0330546.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8V1_002510"
     CDS             complement(593..1714)
                     /locus_tag="ACG8V1_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0330547.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8V1_002511"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8V1_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0330548.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8V1_002512"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8V1_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0330549.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8V1_002513"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8V1_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0330550.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8V1_002514"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8V1_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0330551.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8V1_002515"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8V1_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0330552.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8V1_002516"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8V1_002516"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330553.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKMK010000110         8290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000110 DAZKMK010000000
VERSION     DAZKMK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.112"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8V1_002517"
     CDS             476..1339
                     /locus_tag="ACG8V1_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0330554.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8V1_002518"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8V1_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0330555.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8V1_002519"
     CDS             3339..3860
                     /locus_tag="ACG8V1_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0330556.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8V1_002520"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8V1_002520"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0330557.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8V1_002521"
     CDS             5941..6759
                     /locus_tag="ACG8V1_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0330558.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8V1_002522"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8V1_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330559.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8V1_002523"
     CDS             7228..7806
                     /locus_tag="ACG8V1_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0330560.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8V1_002524"
     CDS             7901..8038
                     /locus_tag="ACG8V1_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330561.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2494 a         1487 c         1634 g         2675 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc
//
LOCUS       DAZKMK010000111         8067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000111 DAZKMK010000000
VERSION     DAZKMK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.113"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..519
                     /locus_tag="ACG8V1_002525"
     CDS             <1..519
                     /locus_tag="ACG8V1_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0330562.1"
                     /translation="KKGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLY
                     TVFNKQTEKFTNIWTESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIP
                     WIDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNE
                     FQDIIHRVDDWI"
     gene            complement(1212..2150)
                     /locus_tag="ACG8V1_002526"
                     /pseudo
     CDS             complement(1212..2150)
                     /locus_tag="ACG8V1_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2150..2767)
                     /locus_tag="ACG8V1_002527"
     CDS             complement(2150..2767)
                     /locus_tag="ACG8V1_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0330563.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(2781..2951)
                     /locus_tag="ACG8V1_002528"
     CDS             complement(2781..2951)
                     /locus_tag="ACG8V1_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330564.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(3299..4789)
                     /locus_tag="ACG8V1_002529"
     CDS             complement(3299..4789)
                     /locus_tag="ACG8V1_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0330565.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(4832..5131)
                     /locus_tag="ACG8V1_002530"
     CDS             complement(4832..5131)
                     /locus_tag="ACG8V1_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330566.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(5497..5781)
                     /locus_tag="ACG8V1_002531"
     CDS             complement(5497..5781)
                     /locus_tag="ACG8V1_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0330567.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(6300..6785)
                     /gene="vanZ-A"
                     /locus_tag="ACG8V1_002532"
     CDS             complement(6300..6785)
                     /gene="vanZ-A"
                     /locus_tag="ACG8V1_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0330568.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(6938..7849)
                     /locus_tag="ACG8V1_002533"
     CDS             complement(6938..7849)
                     /locus_tag="ACG8V1_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0330569.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2437 a         1456 c         1206 g         2968 t
ORIGIN      
        1 aagaaaggat atgaaattta tccctcttta atttatgcaa ttatggaagt tgtaaataaa
       61 aataaagtgt ttagaacagg aattaatagt gagaataaat taggttattg ggataagtta
      121 aatcctttgt atacagtttt taataagcaa actgaaaaat ttactaacat ttggactgaa
      181 tctgataaaa acttcatttc tttttataat aattataaaa atgacttgct tgaatataaa
      241 gataaagaag aaatgtttcc taaaaaaccg atacctgaaa acaccatacc gatttcaatg
      301 attccttgga ttgattttag ttcatttaat ttaaatattg gtaacaatag cagcttttta
      361 ttgcctatta ttacgatagg taaattttat agtgagaata ataaaattta tataccagtt
      421 gctctgcaac ttcatcattc tgtatgtgat ggttaccatg cttcactatt tatgaatgaa
      481 tttcaagata taattcatag ggtagatgat tggatttagt ttttagattt tgaaagtgaa
      541 tttaatttta tacacgtaag tgatcataaa atttatgaac gtataacaac cacatttttt
      601 ggttgcttgt ggttttgatt ttgaatttgg ttttgaactt atggactgat ttattcagtc
      661 cattttttgt gcttgcacaa aaactagcct cgcagagcac acgcattaat gacttatgaa
      721 acgtagtaaa taagtctagt gtgttatact ttacttggaa gatgcaccga ataaaaaata
      781 ttgaagaaca actagcaaaa gattttaaag agttatttta ttttaagtct ttataacatg
      841 agtgaagcga atttttaaat ttcgatagaa atttttacat caaaaagccc cctgtcaaaa
      901 ttgacgaagg gggttttttg gcgcacgctt ttcgttagaa atatacaaga ttgaaaatcg
      961 tgtataagtg cgccctttgt tttgaactta gcacgttaca tcaatttttt aaaatgatgt
     1021 ataagtgcgc ccttttaaat tttgagtgat tatatttttt gagttagaaa aagggattgg
     1081 gaaaatttcc caaaataatt taaaaaataa gcaaaaattt tcgatagaga atgtgctatt
     1141 ttttgtcaaa ggtgtatacc ttgactgtgc ttgctgttac attaagttta tttttaagtt
     1201 attaaaaaag aaatagcttt taaagtttgg ctcgctgtcg ctttataaag ctgattgact
     1261 tttgattgca aactacttaa agaaaacaaa ctcggactat tcgttttctt ctctttggtt
     1321 tgaacatcag caattatccc ctcttgattg cctattttag cttgtttaga agaaacaaaa
     1381 gctaaaagct cctcttgggt tttaaaacgc tgtgtggggc ttagaacgcc cttaaacgac
     1441 ccttggttta cttttatact agcttccacc tcgaaaaaag gttctttttt aaaattctct
     1501 atggcttcct ggcgctgaaa aataaggtat aaggtgggcg tttgaacacg tcctagtgaa
     1561 aatgtacctt gtacgccctt ctgttgtaaa tttaacgtat acaaagggct tgcgttcatg
     1621 ccgatcaacc aatcggcaat ttggcgtgtt tgcgcttctt gataaaaggg atagtaattc
     1681 attccaggtt gcaaattttg aaaaccgctt cggattacat ctttttctaa gctattgatc
     1741 catagtcttt taaatgtttt atcttttgaa aaggcatttg ctttatggat aatcgaccag
     1801 gcgatatttt caccttctct gtcgctatct gttgcaacaa taattgtatt tgcctttttg
     1861 agaagttctg caacaatttt aaactgcttt cccttatctt ttgcaacttc aaaatcgtat
     1921 cgatcaggaa aaatcggcaa agattcaagt ttccaatttt gccacttttc gtcataatga
     1981 cctggttctg ctaattccac taaatgccca aaaccaaagg tgataaacgt ttcatctgta
     2041 aatagtgggt ctttgatctc aaaataaccg tcttttttgg tgctttgttt taaagcactt
     2101 gcgtaggcta atgcctggct tggtttttca gctaaaataa ccgtactcat taactatccc
     2161 tcttttcatt gttttttctt tgatcgactg tcacgttata tcttgctcga taccttctaa
     2221 acgttcggcg attgattcca gtttgttctt caacttcttt atcggataaa ccattcaaaa
     2281 acaaatcgaa agcatgcttt aaacgtggat cattttcttt aaatttatgc tgacgacctt
     2341 tgaatttgcc ttttttctta gcaatttcga ttccttgtgc ctgacgttcc ttaatttttt
     2401 ttcgttctga ttctgcttga tacttgtaca attcaatgac aaggctatta atcaaacgcc
     2461 ttaaattttc atcttcaata ccattcattg agggtaaatt taagacttcc agggttgccc
     2521 ccttaatttg aatttgattc atcaattctg ttaattcttt attatttcgt cctaatcgat
     2581 ctaattcagt aacaataaca atatcccctt cacgaatata gttaagcata gcttgtaatt
     2641 gtgggcgttc gaccgattga ccgcttaatt tgtctgaaaa gaccttagaa acgccctgta
     2701 acgcttgtaa ttgccgatct aagttctgtt ctttgctact gacacgtgca taaccaattt
     2761 tagccatttt caaccaacct ctaaaattct ctcggttgca ataaccaatc agcaatatct
     2821 actttttcaa tttcaaattg cttatcagaa attgtctttt cgtaagcgat aaaatcttgc
     2881 gcatattgtt gctcattaaa aatagccacc acttcgtcat tttctaaaac tcgataaata
     2941 aattttttca ttttactcct cctattatgc ccaacttaaa tgacctattc accaagtcaa
     3001 ttatactgct aaaatcatat taggacaaat aggtatactc taatgaccta taaaagatag
     3061 aaaattaaaa aatcaagtgt tcgcttcgct ctcactgccc ctcgacgttt tagtagcctt
     3121 tccctcactt cgttcagtcc aagccaacta aaagttttcg ggctactctc tccttctccc
     3181 cctaataatt aattaaaatc ttactctgta tatttctgct aatcattcgc taaacagcaa
     3241 agaaaaaaca aacacgtatc atagatataa atgtaatggc atagtgcggg ttttattttc
     3301 agcctgtatc atagctaaac aaatcgagtt gtgtgtccgt tttagggcgt tctgctagct
     3361 tgtttaaagt ctcttgaatg aatgtatgct ctaagtcaaa agaatttgtc agcgccttta
     3421 tatagctttc tttttcttct ttttttactt taatgatcga tagcaacaat gatttaacac
     3481 tagcaagttg aatgccacca tttcttcctg gtttaatctt aaagaaaatt tcctgattcg
     3541 ccttcagtac cttcagcaat ttatctaatg tccgttcagg aatgcctagc acttctctaa
     3601 tctctttttt ggtcgtcact aaataaggct tgtatacatc gcttttttcg ctaatataag
     3661 ccattaaatc ttctttccat tctgacaaat gaacacgttg acgttcgctt ctttttttct
     3721 tgaatttaaa ccacccttga cggacaaata aatctttact ggttaaatca cttgataccc
     3781 aagctttgca aagaatggta atgtattccc tattagcccc ttgatagttt tctgaatagg
     3841 cacttctaac aattttgatt acttcttttt cttctaaggg ttgatctaat cgattattaa
     3901 actcaaacat attatattcg cacgtttcga ttgaatagcc tgaactaaag taggctaaag
     3961 agagggtaaa catgacgtta ttacgcccta ttaaaccctt ttctcctgaa aatttcgttt
     4021 cgtgcaataa gagattaaac cagggttcat ctacttgttt tttgccttct gtaccgctta
     4081 aaaccgttag acttgaacga gtaaagccct tattatctgt ttgtttgaaa gaccaatctt
     4141 gccattcttt gaaagaataa cggtaattag gatcaaaaaa ttctacattg tccgttcttg
     4201 gtatgcgagc aataccaaaa tgattacacg ttagatcaac tggcaaagac tttccaaaat
     4261 attctcggat attttgcgaa attattttgg ctgctttgac agatttaaat tctgattttg
     4321 aagtcacata gactggcgtt tctaaaacaa aatatgcttg ataaccttta tcagatttga
     4381 taatcatagt aggcataaaa cctaaatcaa tagcggttgt taaaatatcg cttgctgaaa
     4441 tagtttcttt tgccgtgtga atatcaaaat caataaagaa ggtattgatt tgtcttaaat
     4501 tgttttcaga atgtcctttc gtgtatgaac ggttttcgtc tgcatacgtt ccataacgat
     4561 aaacgttggg tgtccaatgt gtaaatgtat cttgattttc ttgaatcgct tcctcggaag
     4621 tcagaacaac accacgaccg ccaatcatgc ttgattttga gcgatacgca aaaatagccc
     4681 ctttgctttt acctggcttg gtagtgattg agcgaatttt actattttta aatttgtact
     4741 ttaacaagcc gtcatgaagc acagtttcta caacaaaagg gatattcatt cagctgttct
     4801 cctttcctat aaatcctata aaataggttg tttaattaac ttggtttgct ttttcattca
     4861 actgtttcaa tattgcatgt tttgaaaaag atttttttcc tttataagtc aatttttttc
     4921 cactaatcga ataaattatt ttgttatttt ctattaactt atatatataa tcttccccct
     4981 ccgaagaaaa atacttatct gattttgttt ctaagtagat atttctcttt tctaactctt
     5041 tcttaaacgt ttctagtgta tagatatttg ctaattttct tatctccaat aaactatttt
     5101 ttatataagt tttacattca tcatgattca tacaaactcc accttctata aatgaataca
     5161 aaaaaagcaa tcaaacgatt tccgattgat tgcttaacaa ttcttaaatt cagtagctta
     5221 gatacttgaa aactctctga tttccctata taatgatagt acggttatat accgtcttca
     5281 aacaaagtta attaaataac ttcttacgag ggaagagttc atctgactaa ctgataagcg
     5341 ttggtttggc aatcttatcg ggctatgcat ttataaaatg tcgtcaaaca ttttataaat
     5401 gtgtcatggc tcttttttcg tttctattca gttcgttgtt tcgttatatc tagtataccg
     5461 cttttaaaaa aaataagcaa cgatttcgtg cattattcac acgaagtcat tgcttttttc
     5521 ttcttccatt tctaaatcca atgttacttg ttctgattct gtttctggtt ctggttctgt
     5581 tggctcattt gggattaaat ccactactag cgttgagtta gttaactttg caatttgttc
     5641 tagtgttttt atggttggat ctgattttcc tgattctatt cgtgaataat ttgatctact
     5701 catttctaat tcttgggaga atttttcttt tgatttggta cctctcatct tttttaagct
     5761 ttctctaaat gctagttcca agtttctccc ccctcttttt gtgtaataaa attcacgctt
     5821 tatttaaatt atagctttta atatcttaat aaacaagtga ttaatcactt gtgatttgtg
     5881 attattccaa aagtaggtaa tttcaccaac ctttttcaaa agtttttatc gacgattatt
     5941 ggactggatc gtgtttccta aagcgattct aagcgcatgt ttaattttaa agtgggtaat
     6001 tctctttaaa acgtcctaca aagattcaaa aaaagcatgg ctgctagtgg gggtaccgtc
     6061 aggaaaatgc ggatttacaa cgttaaggaa atttattaaa gagaccaatt cctcagcact
     6121 aaaattgata attgggcttt ctttgttact atagtgttag gtaagctttt tactttatca
     6181 ccggttttcc cctcacttca cacctacggg cgagtttccc cacatcccgt tttccatcta
     6241 atccaattca ttcaatatct ttatgtgtat gaaagatcag attgcttgat ttagacatct
     6301 tacacgtaat ttattcttaa atgggtacgg taaacgagca ataatacgag caaaagtata
     6361 cctacaaaaa taataactct gtctaatttt ttttgattca tatgcttatt gcttaaacca
     6421 tataatttca gtccaagaaa gcctccaaca gtatttgtaa ttacatctgt tatgtctgtc
     6481 gctccaatag cgaagataaa ttgaattatt tcaaaagtaa gacttaaaac cagtacaaaa
     6541 gcaaacttag gtaaaaatcc gatttcttta aaattgacat tcaaaagcaa gccaaatgga
     6601 ataaagatta taacattatc tatcatctct ctgaaattcc cagtagcagt aaatggagtc
     6661 aagttaagac ttctttgatg ataattaaat actgataaaa tattgtattg taatttgaat
     6721 aacactaacc agattagtgt cactaaatat aaagctagca atcctctaga taatattttt
     6781 cccaatataa tttcctctaa ttcttttgtt taagttgtat cattagtata gcaatgtcct
     6841 agttaacgtg ggttttaaat taaaaagcta acgttacccc aaaagtgcta gtacaataat
     6901 gctgttcatt aatttcgttg gtttcattcc gccatcctta cctccttgaa ttagtatgtg
     6961 ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat atctcataac
     7021 gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa aagatctcat
     7081 atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa tccatatatt
     7141 cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca acatagcgaa
     7201 tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga taacgtaaaa
     7261 tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct cgttccattt
     7321 tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta taacctgctg
     7381 gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca aagtctcgat
     7441 agccactatt aataataaaa tgactaacgc caccctttac agcatcattg accatctctg
     7501 aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac ccgtatccat
     7561 ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt tcttggcgaa
     7621 caggatattt actattgatt aatagcagat ttccttgata aacctgttct tctgtaattg
     7681 ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg ggattttgat
     7741 cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg tagtcataac
     7801 ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt ttcagtctcc
     7861 tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt aaacggcaca
     7921 atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt tcgccctact
     7981 ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat gctgaattta
     8041 acgaggctga actgcctgtt gacaatg
//
LOCUS       DAZKMK010000112         7590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000112 DAZKMK010000000
VERSION     DAZKMK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.115"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..417)
                     /locus_tag="ACG8V1_002534"
     CDS             complement(127..417)
                     /locus_tag="ACG8V1_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330570.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(535..795)
                     /locus_tag="ACG8V1_002535"
     CDS             complement(535..795)
                     /locus_tag="ACG8V1_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330571.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(932..1345)
                     /locus_tag="ACG8V1_002536"
     CDS             complement(932..1345)
                     /locus_tag="ACG8V1_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0330572.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1801..2286)
                     /locus_tag="ACG8V1_002537"
     CDS             complement(1801..2286)
                     /locus_tag="ACG8V1_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330573.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2425..2640)
                     /locus_tag="ACG8V1_002538"
     CDS             complement(2425..2640)
                     /locus_tag="ACG8V1_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0330574.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2864..3664
                     /locus_tag="ACG8V1_002539"
     CDS             2864..3664
                     /locus_tag="ACG8V1_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0330575.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3683..5551
                     /locus_tag="ACG8V1_002540"
     CDS             3683..5551
                     /locus_tag="ACG8V1_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0330576.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5544..6035
                     /locus_tag="ACG8V1_002541"
     CDS             5544..6035
                     /locus_tag="ACG8V1_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0330577.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6023..6577
                     /locus_tag="ACG8V1_002542"
     CDS             6023..6577
                     /locus_tag="ACG8V1_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0330578.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6595..7590
                     /locus_tag="ACG8V1_002543"
     CDS             6595..7590
                     /locus_tag="ACG8V1_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0330579.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1212 c         1442 g         2230 t
ORIGIN      
        1 gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac ttcaaaaaag
       61 ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt aatacatttt
      121 aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa gagagatcat
      181 aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat cccatatttc
      241 ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga tccagcacca
      301 tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg gaagtaaaaa
      361 caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct cccccacatt
      421 caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg tatgaataac
      481 agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg aagtttattt
      541 ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata agccgatttc
      601 gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct tatggataaa
      661 atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga ataagtaaaa
      721 gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta agacaatcaa
      781 atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg taaaaacaaa
      841 atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa tatataaaaa
      901 tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt tttgtttctt
      961 ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta aaggttccag
     1021 tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag accgaatgtc
     1081 tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca tgattggcgg
     1141 agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca cagccaggca
     1201 tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt ggataacttg
     1261 aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca gggaacatgt
     1321 cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat tttttattta
     1381 tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt tagctttgtg
     1441 aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg gatcattaaa
     1501 ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc aaatatagaa
     1561 aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga ccgaaaatga
     1621 agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt cataagagaa
     1681 atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt ataactttct
     1741 cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa tatcatctaa
     1801 tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg acactcgata
     1861 aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag ctgtgaacaa
     1921 ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt gtaattcgga
     1981 acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa gagaaggtac
     2041 agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac ttgattggct
     2101 atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag aagtactttt
     2161 agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag aattctttgt
     2221 tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt aaatcccttt
     2281 atgcatgata gctcctccta aaatagatta tctttattca gtatatcact taaacaaaga
     2341 aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca tgaagataat
     2401 aactttttta ataattatag ctttctaata tctttcatca acttttctag taatcattgg
     2461 gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga tcttcgcaaa
     2521 aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca agcctaccaa
     2581 cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta aagttttcat
     2641 tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa acattgctta
     2701 tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt ttgctcttcc
     2761 attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt tttagtgagc
     2821 ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg attggttgga
     2881 tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa gaagtatcgt
     2941 cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg aatgccgtat
     3001 acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg acatagaaga
     3061 agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca ataatgcagg
     3121 aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt atgaattatc
     3181 agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca
     3241 agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga
     3301 atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag cagcgatgaa
     3361 cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt
     3421 tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag aagctctttc
     3481 ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa caagtacgat
     3541 ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct attatctttc
     3601 agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta aaacaagagg
     3661 ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat tattgaaatt
     3721 actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa
     3781 tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta ttgcaaaaat
     3841 catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt ttgatgagat
     3901 catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa gaatcgctta
     3961 tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt tgtctgaaga
     4021 agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag agatgatcaa
     4081 tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa taggagatga
     4141 gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag agacgaaaac
     4201 agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga cgaatgctag
     4261 taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata gaatctctag
     4321 taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat atatccaaga
     4381 attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga gccaatatga
     4441 aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga tgataaggaa
     4501 agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc gacgattaga
     4561 tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc tctctttatt
     4621 gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg agatagaaaa
     4681 aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag aagctatctt
     4741 gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg agttagcatt
     4801 tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata cgacaggaaa
     4861 aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg atatacaaat
     4921 aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt tcgctgtttt
     4981 tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt tgactaatct
     5041 ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg
     5101 gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt atcaagaata
     5161 tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag aatttgaaaa
     5221 aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat ttgcttttcc
     5281 tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa taactgatca
     5341 aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac ctgaagaaat
     5401 gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg aactcataaa
     5461 aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt taaaactaat
     5521 caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt ttcgcagctt
     5581 cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc aatcaaacat
     5641 atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg aaaatccgtt
     5701 caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa gtcagaaaaa
     5761 tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg ggtgaagata
     5821 tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg acacaagcag
     5881 ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa aaaagtgaac
     5941 aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa agtacgatca
     6001 aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg gatttatgag
     6061 gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg taccagttgt
     6121 cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag accgagtaac
     6181 gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt taccgttctt
     6241 atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc ttccagaata
     6301 ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca atggtgtatt
     6361 cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag ggatcgaaac
     6421 cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac tgctgatgaa
     6481 ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac agcaaggaat
     6541 cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt ttaaatgtat
     6601 ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat tattaggccg
     6661 acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc aatgatcgta
     6721 gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac atcaatacct
     6781 gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg tggaatttat
     6841 cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg cccattagca
     6901 caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat acagccacta
     6961 atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa aaagtacaaa
     7021 tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa
     7081 attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag agaagcagta
     7141 gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt gattggtgtg
     7201 atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt agcaggaaat
     7261 gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc tcctttgctt
     7321 ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga gattgggaaa
     7381 ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac tcaaaatgca
     7441 tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac agtagaagta
     7501 ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt tatcattgct
     7561 tgggtagcaa gttttggatt atatagctga
//
LOCUS       DAZKMK010000113         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000113 DAZKMK010000000
VERSION     DAZKMK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.116"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG8V1_002544"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG8V1_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0330580.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG8V1_002545"
     CDS             1483..1935
                     /locus_tag="ACG8V1_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330581.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG8V1_002546"
     CDS             1949..2638
                     /locus_tag="ACG8V1_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330582.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG8V1_002547"
     CDS             2763..5114
                     /locus_tag="ACG8V1_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0330583.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG8V1_002548"
     CDS             5194..5589
                     /locus_tag="ACG8V1_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330584.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG8V1_002549"
     CDS             5914..6531
                     /locus_tag="ACG8V1_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0330585.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG8V1_002550"
     CDS             6572..6871
                     /locus_tag="ACG8V1_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0330586.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG8V1_002551"
     CDS             6905..7072
                     /locus_tag="ACG8V1_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0330587.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG8V1_002552"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG8V1_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0330588.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKMK010000114         7428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000114 DAZKMK010000000
VERSION     DAZKMK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.117"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2614
                     /gene="clpB"
                     /locus_tag="ACG8V1_002553"
     CDS             5..2614
                     /gene="clpB"
                     /locus_tag="ACG8V1_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0330589.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2765..3451)
                     /locus_tag="ACG8V1_002554"
     CDS             complement(2765..3451)
                     /locus_tag="ACG8V1_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330590.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3429..3923)
                     /locus_tag="ACG8V1_002555"
     CDS             complement(3429..3923)
                     /locus_tag="ACG8V1_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0330591.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(3943..5163)
                     /locus_tag="ACG8V1_002556"
     CDS             complement(3943..5163)
                     /locus_tag="ACG8V1_002556"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0330592.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5310..7145
                     /gene="lepA"
                     /locus_tag="ACG8V1_002557"
     CDS             5310..7145
                     /gene="lepA"
                     /locus_tag="ACG8V1_002557"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0330593.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
BASE COUNT         2452 a         1407 c         1516 g         2053 t
ORIGIN      
        1 ctgtatgaat attgagaaaa tgactacgac gcttcaagaa gcgattgctg aagctcaaca
       61 gattgcggtc actcgaaaac atcaagatat cgacattgcc catgtatgga aaatcttttt
      121 acaatctaat cattttggtc gaaatttcta tacggatgcc ggattggatg tcgaatcttt
      181 tgaacatgaa attgatcgtc tactggatga gtatccagtt gtttctggtg gaaacgtcca
      241 atatggacaa aacctcagcc agaatttctt tcgattattg aatgaagcag accaaatacg
      301 tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt
      361 aaaaaattat ccgttaactg tttatttgaa taaaaacgga ttatctgaaa aagaattgcg
      421 taaaaacatc gaagagatga gaggaggaga aagagtgacc tcacaaaacc aagaagaaca
      481 atacaaagca ttagaaaaat acggcgttga tttagtacag caagtcaaaa gcggaaaaat
      541 ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa
      601 aacaaaaaat aatcctgttc tgataggaga acccggtgta gggaaaacgg caatcgttga
      661 agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat
      721 tttttcatta gatatgggtg cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga
      781 aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat
      841 cgatgaaatc cacaatatcg taggggcagg aaaaacagaa ggaagtatgg atgccggaaa
      901 tctgttgaaa ccaatgcttg ctcgcggtga attacatttg atcggtgcca caactttaga
      961 tgagtaccgt caatatatgg aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt
     1021 agtcaaagaa ccaacagtgg aagatacgat cagtattctg cgtggattaa aagaacgttt
     1081 tgagatccac catggtgtta acatccatga caacgcatta gtagcagcag ctactttatc
     1141 tgaccgttat attactgatc ggttcttacc ggacaaagca atcgatttga tcgatgaagc
     1201 cagtgcaacg attcgggtag aaatgaactc tatgccaacg gaattagatc aagttactag
     1261 acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag
     1321 taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc
     1381 gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc
     1441 tgaaatcgat aaagcaaaac acgaattaga agatgcagaa aataactatg atttagaacg
     1501 cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa aaagaattga aggaattaga
     1561 agctaaagca aaagacagcg agattaagat ggttcaagag tccgttaccg aaaatgagat
     1621 tgctcaagtc gttggcagat taacgggtat ccccgtaacg aaacttgtag aaggtgaacg
     1681 agaaaaacta attaaattga atgaaacact tcataaacga gtgattggtc aagatgaagc
     1741 agttgatgcg gtcagtgacg cggtaattcg ttcacgggct ggattgcaag acccaaatcg
     1801 tcctctaggt tctttccttt tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa
     1861 agcgttagct gagaacttgt ttgactcaga agatcatatg gtacggatcg atatgagtga
     1921 atatatggaa aaacatgctg tgtctcgtct agtcggagct cctccaggct atgtaggtta
     1981 cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt
     2041 ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc ttgttacaag tcctagatga
     2101 cggacgcttg actgattcaa aagggcgagt agtagatttc aaaaatacgg ttctgatcat
     2161 gaccagcaat atcggttcac agttactgct ggaaggcgtg actgctgatg gaacgattcc
     2221 agaagcagtg gctgaacaag tcaacacgct attacgtgga aacttcaaac cagaattctt
     2281 gaaccgaatc gacgacacga ttttatttac accattgagc ctagacaacg tgaaaggaat
     2341 cgttgacaaa atggtggcgc aattggcaca acgtctggaa catcaagaaa tcctgctgac
     2401 aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct tatgaaccag catatggtgc
     2461 aagaccattg aaacgattta tcactaagga agtagaaaca ccactagcta aagaaatcgt
     2521 tgcaggacat gttatgccta aatcaaaagt aacgattact ttacttgatg gacaacttca
     2581 tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc attgagacta taaaagaaaa
     2641 aaggggtctg acacaactag tgtcagacct ccttttttca aataaacagt gttccagaag
     2701 tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct
     2761 tttattaggt atttttcact ttttctaatt tataactttc aaagtaacgc tcatcttttt
     2821 tactttgaga ccatgcaaac aatcccatta aggcagcacc tatcgtattt gtcagcaagt
     2881 cgcccatcgt atccatcaat gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa
     2941 aacgttgaag attcatgcct aagaactggt cgcataagaa ttcccagaac tcccaaaaca
     3001 cgccgcaaag tccagcaaac gcaaagccga ataagaggaa caaccaagga ctggtttttg
     3061 atatctcagc atctttcatc aagtaaccaa tcaatccata gccaagagct gtcaggacca
     3121 ttggactcac cgcatgtaag attttgtccc aaaaagagat gatactgatc aaatgcatcc
     3181 ctgtgcctaa aaagacagag atgaacaaga aaaaccagta aaacaaaacg attgctgctg
     3241 gtattttaag tttgaaaact ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta
     3301 ataaagattc cacgataaat gtgattcctt gagaagtcga atatttttgt gtgataaatt
     3361 cataaaaatt atagcagaca acgaatacac caaaagcaag taaagctatt tgcacctttt
     3421 tgatttcttt aatcactgaa agtccctcca tctaaaaatc tttttacttc tgaaagcaac
     3481 tcaatttctg aagaaaccac ttttccgttc aatttgatca aacctattgt atataagttc
     3541 aggtagtgaa attgattttc agctgtttct tgtagtgcag cgattttttt cgggtgggtt
     3601 ccgccttgct gtcgagtatc agtatataag ccgatcactg ggatgccttt cgcataagcc
     3661 acaccgattt ccgatgctac cccggcatcg atcgtcagcc catctagtaa agcaatcatt
     3721 aaatcactag ccaacacttt ttctgtgtca gcaagagcaa tcattcgact gtctgcataa
     3781 gcagttttgt cattgattgc ttcattttct tgaggaagat agatcgaaag ctcagaagag
     3841 agctctctga tttctttaac taactgttgg ttatataaca aatcactttt agcaaacatt
     3901 ggtgcggcaa aataaatttt catctaaaca tcatcctttt tatcaaaaat aatcgctaaa
     3961 gtaagcttcc atgtttggaa tgatacgctc tagtgacttc agtgtttctt catctgtttc
     4021 taaccgttcg attcttgcca aataagaagc acggcgataa ttcatcatca agcaagtcaa
     4081 tgtttggata tccagtcgaa cggattttcc tataggttca tttaagacac ggacttgtcc
     4141 ctgttcatcc catgttaaag cgaagacacc attattccaa ggagcgacag gatcttcaat
     4201 gatgaaatga aaagcatcag ctgttccaac aaacggaaac cgttgaagga attcttttac
     4261 atcaacgatc cgagccataa aatatggttc gatctgctct ttgatctgac tatcttcaat
     4321 caaaaatgct aaaggctcat ttttataaat atctccatgt acccagtaga tcatggaaaa
     4381 atgtgcggaa ataaagttcc ataagccatt tcgagcttct tgatcaagat agaacatttc
     4441 ttttacgtga aatatttctt cagctaccca gtagaacaga acacctcttg gttcatggtt
     4501 gctatcgtag tagacagcgg ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc
     4561 ccaatgaaaa tcactgcgga ataaagcacc atggttttgt tgggcaaatt tgtcataaac
     4621 ggaaaatacg tctggatgat caactggtaa tcgctcaacc ataccactta ctgggatttg
     4681 tttaggtagc tgtgtatctc tgattttaaa ggaaagttta tcagacatga tttcccatcc
     4741 tttacgccga tagtagggga tactgtaagg atacaaataa gagatccatt gcttattctt
     4801 gcgcatatta tccaatgcaa cgataattag atcttgcatc aatccatggt tagcatattc
     4861 tggataagtt ccaactccag tcacaccacc cattttataa cgtgcaccgt gaatattgac
     4921 ttcacaagga tagatagcga tttgagagat cagtttattt tcgtggaacc atccgaacac
     4981 tttcgatact tccaaaatcg gttttttgga acggataaaa gctcgtttat tctcaaaccc
     5041 gctttcctcg atgtctgctt cagtcacttg gaacacatag gaaagcaatt cattgaattg
     5101 atcgatatgt tcttgagtga ccggttttaa ttcaagattt tctctaaaat ctttttgatc
     5161 cattttttca tctccattta ttagcttgat aacagtatag caaaattcta caaaaaaaac
     5221 ttctatagct gactaattaa aaactcttta aattactgct tttcgttggt ataatggaaa
     5281 gtgctgatta tatgaaaaga ggagacgcta tggatataaa gaaaatgaaa gaacgccaag
     5341 agcagattcg taatttttcg atcatcgctc atatcgacca tgggaaatct acattggccg
     5401 atcgtatttt agaaatgacg cacactgtga cgtctcgcga aatgcaagat cagctgttag
     5461 actcgatgga tttggaacgt gaacgtggaa tcacgattaa attaaatgct gtagaactca
     5521 attacacagc gaaaaatgga gaaacatata tctttcactt gattgacacc ccagggcacg
     5581 tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg
     5641 tcgatgcagc acaaggaatc gaagcacaaa cattggccaa tgtttattta gcgttagaca
     5701 atgatttgga gatcctgcca gtcatcaata aaatcgacct acccgcagca gatcccgaac
     5761 gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc tagtgaagct gtattagcca
     5821 gtgccaaatc agggattggg attgaagata ttctcgagca gatcgtggaa tatgttcctg
     5881 caccaagtgg agacttagat gcaccgctaa aagctttgat ctttgactct atttatgata
     5941 gttaccgcgg tgttgttcta aatgtccgga tcacggacgg tatggtcaaa cccggagaca
     6001 aaatcaaact gatgagtaat ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc
     6061 caaaagcagt tgctagagac tttttgatgg tcggtgatgt cggttatatc acagcaagta
     6121 tcaaaactgt ccaagataca cgagtagggg atacagtaac gttagcagac aatcctgccg
     6181 ctgaagcttt accaggttac cgaaaaatga atccgatggt ttattgtggt ctttatccaa
     6241 ttgatacttc tcgttacaac gatttgcggg aagctttaga aaaactgcaa ttgaatgatg
     6301 ctgctttaca attcgaacca gaaacctctc aagcattagg ttttgggttc cgctgtggat
     6361 tcctcggact tcttcatatg gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag
     6421 aattgatcac gactgcacca tccgttattt accatgttaa taaaacggat ggtacgactg
     6481 ctacagtaga taacccggca gattttcctg aacctgtaac gattcaggat gtagaagaac
     6541 cattcgtcaa agcacagatc atggtcccaa atgattttgt cggtgcagta atggaacttt
     6601 ctcaacgaaa acggggagaa ttcattacga tggattattt agatgattac cgagtaaatg
     6661 tcgtctataa tattccgctt tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa
     6721 gcacgaaagg ctatgcctca ttggattatg aaatgtctgg ttatcaaaaa agcaagttag
     6781 tgaagatgga catcttgctg aatggagaaa aagtcgatgc attaagcttt atcgttcacc
     6841 gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac
     6901 gccaacaatt tgaggtgcca atccaagcag ctattggaca aaaaatcgtt gcccgttcag
     6961 acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta tggtggagat gtttctcgta
     7021 aacgtaaact cttagagaaa caaaaagaag ggaagaagcg gatgaaacaa atcggctccg
     7081 tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat ggatgagcaa gatagtgtga
     7141 aataacttat aatcaatcga aaaacctatt aaataaacct ttttgagtag atgcaacact
     7201 gaggattatc tgtataaaag attttaaact gtcatttggt tatcacggcg atactaaaat
     7261 taacaaaatt ggaatatttt tcttgaagat ttttagctgt cagtatatga tgaaagcatc
     7321 tcataggagg tgcttttttc atggttgata attcaagtgg aagggttctt aaatctctaa
     7381 gaaaagagaa aaaactatct caaaaaaagt tagctgattt agcaggta
//
LOCUS       DAZKMK010000115         7217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000115 DAZKMK010000000
VERSION     DAZKMK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.118"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG8V1_002558"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG8V1_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0330594.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG8V1_002559"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG8V1_002559"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0330595.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG8V1_002560"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG8V1_002560"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0330596.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG8V1_002561"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG8V1_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0330597.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG8V1_002562"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG8V1_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0330598.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACG8V1_002563"
     CDS             complement(4433..5290)
                     /locus_tag="ACG8V1_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0330599.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG8V1_002564"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG8V1_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0330600.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DAZKMK010000116         7198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000116 DAZKMK010000000
VERSION     DAZKMK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.119"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..920
                     /locus_tag="ACG8V1_002565"
     CDS             24..920
                     /locus_tag="ACG8V1_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0330601.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(975..2345)
                     /locus_tag="ACG8V1_002566"
     CDS             complement(975..2345)
                     /locus_tag="ACG8V1_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0330602.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            2583..3329
                     /locus_tag="ACG8V1_002567"
     CDS             2583..3329
                     /locus_tag="ACG8V1_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330603.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            3326..4165
                     /locus_tag="ACG8V1_002568"
     CDS             3326..4165
                     /locus_tag="ACG8V1_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0330604.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            4166..5116
                     /locus_tag="ACG8V1_002569"
     CDS             4166..5116
                     /locus_tag="ACG8V1_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0330605.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            5257..6009
                     /locus_tag="ACG8V1_002570"
     CDS             5257..6009
                     /locus_tag="ACG8V1_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330606.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            6028..7179
                     /gene="queG"
                     /locus_tag="ACG8V1_002571"
     CDS             6028..7179
                     /gene="queG"
                     /locus_tag="ACG8V1_002571"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0330607.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         2500 a         1283 c         1500 g         1915 t
ORIGIN      
        1 cataaaaatt ggtggtgaaa aaaatgtatg atgaaaatga gtggggagaa aatgttcgga
       61 tgacccgaag aagacagcaa aaacaggcag ctcgtaagcg gatgatcatg attggggcag
      121 cagttgccgt gtttttgatc atagcaggag gagttgcggg tctatatttt catggaaaaa
      181 attcgtcaga agcgaaaagt acgaccataa cggaaacaac gacaaagaaa acagagacga
      241 aacaaacaag tgagtcatca actaaaacaa aagagacaca aacttctgaa gaaaaaacag
      301 caaatggttt gccagagcaa cttcaagcaa tcgtaaatga ttattcgaaa aagatcgaag
      361 aaaagacgcc tgcattgatt gaagaatacc agacagaaat tcagggaaat caagagggaa
      421 tagctggttt atcagcagtt gctaaccaaa aagcacgtga gcttcaagca atctccgacg
      481 aagggatcag aaaattacgt gcagcgtatc aggcagcaga aaataaagac ggtgtagact
      541 tagatacttt gatcaatcaa ttatcggcta attatacgaa tcatgtcgct aagatttccg
      601 atatttattt gcagactagc gcctctcttc aagcagagtc aacaagcaca caagacacaa
      661 catcttctga ttctgagaca gcagagtcgg tagaagcttc tgaaatgcaa gctcgaacaa
      721 cacaagacac aacagattcc tctgaaacta ataccaacag cgcgacttca caggctgcga
      781 caaccgtagt tttggaagga gaaggtccaa atcaaatagc cgaacgaact ggcgtaccag
      841 tagaaacgat cttgtcgctg aatgggatga gcatggagaa ttatttcctg actcccggtg
      901 agacgctaaa attgaattga taaaagcaat aaaaaagaac gaagcttcca ctaaggtgaa
      961 gcttcgttct ttttttattt tccggttctt ttttcgcgta caaagtaatg gatcgtcatg
     1021 taaagcaaga aaacgagtcc aataatcaat gagattctcg tttcaggatt gataaacatg
     1081 aaaatcaata cgatagctaa aaaagcaagt gtcacataat tcgtataagg agatagtggc
     1141 attttaaaag gatgatcatg cattttatct gcgtgcttct ttttgaagcg aatctcgctg
     1201 atcaaaatca ctacccaagg aaccatacca ggcaaaatac ttgagctgta tacgaaaacg
     1261 aaaatatttt ctgactggtt gatgaacatt ggtagaagat aatttaagat cagaccaatt
     1321 aagattccac ctgagattgc tacgacagga taaaaaggcg caccgtgacg agatagtttc
     1381 agaaattttt tagacatctt tccatttatg gcaagtgtat agaccatacg acttgcgctg
     1441 aaaatccctg aattgcaacc tgataatgcc gctgtaatca cgacaaaatt aatcagtcct
     1501 gcagcaaatg taatgccgac tttagaaaaa gtttgtacaa aaggtgaacc aatctcactc
     1561 aattgattcc acggatagat actcacaatg ataaaaatag caccaatata aaaaatcaag
     1621 attcgaccga tagtggactg gacagcttca atgatcgtct ttttaggatt ctctgcttca
     1681 ccagcagtca ttccgatcag ttcgattcct tgataggaag caacaacgat cgataatgca
     1741 aagaaaaatc ctttgacgcc tccagtgaag aatccaccat ttttccaaag atttgaaatc
     1801 cctactgcct gaccatgatt accaaatcca aataagatta ccccaagacc tgcaatgatc
     1861 atcaaaatga tcgttatgac tttgatgatc gaaaaccaaa attctaattc gccaaacgcc
     1921 ttgacagaaa taagatttgc cagcataaga aacaagacga caatgattcc gggaataatt
     1981 gccggcaaat caggccacca ataattcata taagatccta cagcaatcac ttcacttatt
     2041 cctacgacaa tatattgaaa aatattactc caagcggtca agtagccagc cacaggatgg
     2101 atgtactcgc tcgcgtaatt tgcaaaagaa ccagtcgaag gatcaacgta aagcatttcg
     2161 cctaaagcac gcataatcaa atacaaaaag aatcctgcaa tcgcataggc tcccatcact
     2221 gagggccctg tccaacgtat ggttgaggag gcacccataa acagaccgac accgatcgtt
     2281 ccacctagtg cgatcatttg gatatgacgg gcactaagac ttcgagttaa cttttcttct
     2341 tccatacaaa aactcctttc gttgttgagt caacaagaaa aaataatcaa acgtttcaac
     2401 acatgttttt cattattagg ttagccttat tgaactcttg atgtgattct acagaaaatt
     2461 ttcataaaat gcgaggaaaa ttcgaaaaaa actttttcat cattctactt gaaaatgctt
     2521 tccattttag ttaaactata tttaaataaa aagttaggta aacctaaaaa ggagtgtaag
     2581 atttggaacg tacggccatt gtaattcaag acctttcagc cgcttatcaa gggaaaactg
     2641 ttttgaaaac tatcaacttg acgataaatc cacaaaaaat taccggtatt atcggaccaa
     2701 acggtgcagg aaaatcaaca tttatgaaat ctttgctaga gttgatacca gctactactg
     2761 gtaaagtcac cttttctgga cagtcggtaa actcagtccg taaaaaaata gcctacgtgg
     2821 aacagcgaag tgaattggat ctttcctttc caattgatgt attaggcgtt gtacttttag
     2881 gaacatatcc atctttacga attggacaaa gacctgggaa aaaagaaaaa gaacgtgcaa
     2941 gacaagcttt gaaaaaagta gggatggaag aatatgccaa aagacagatc agcgaactat
     3001 cgggtggaca gcttcagaga gtttttattg caagagctct agcccaagga gcagaatgga
     3061 tctttttaga tgaaccattc gtagggattg atgcgttaag tgaacgaaag atctttgaca
     3121 tcttgcagga attgaagaat tcaggaaaaa cgattttgat cgtccatcat gatcttcata
     3181 aagtagaaga atatttcgat gaggttattc ttttaaataa acagctgatc gcttccggtc
     3241 cagtacaaga agtctttaca tcgaagaacc ttcaattggc ctatggtgag atcatcagac
     3301 atttagtaaa aggaggagag aaaaaatgat ccaatccttt attgatggat taatgaatta
     3361 tcaattcctg caatacgctt tggtcacttc catgcttgta ggacttgctt ctggagtcat
     3421 cggttctttt attattttac gagggatgtc tttgatggga gacgctatct ctcacgcagt
     3481 tcttccaggt gtagccattt cttatatgtt tggttttagt tacatctttg gagccactgc
     3541 ttttgggatg ctaacagcag cagtgattgg tttcgtgact cagcggagcc gattgaaaaa
     3601 tgatacagcg attggaattg tctttagctc cttttttgca ttagggatta tcttgatttc
     3661 atatgcccag agtgcgacag atttgtatca tattttattt gggaatgtct tagctgttag
     3721 ggaaagcgat ttgttgctga cagcactcgt tagtggaatc gtcttgatat ttgtcttttt
     3781 cttttataaa gaattgaaga tcacatcatt cgatccgaca atggcaaaag cttatgggct
     3841 gaacacatcg ctgatccatt atcttttgat gttctttttg acattagtgg ctgtagtcag
     3901 tttacagaca gtaggaacaa tcttggtgat tgccatgttg atcacaccag ccgccacggc
     3961 ttacttgcta acgaaccatt tactgaaaat gatcattaca gctgcaggaa tcggtatgct
     4021 aagtgcagtt gtcggtgtgt ttttcagtta tagttacaat tggccatcag gagctacgat
     4081 cgtgttagca tgtaccgcat tttttatcct tgctttttta ttttctccaa caaaaggaat
     4141 tttatttcag aaaggaagca gatagatgaa aacaaaaaaa tcactctttt taatcttggc
     4201 agttagtttc ttggtattag ccggttgcgg aaagcaggct tctgaccaag cggatgaagg
     4261 aagtaaagaa aagttgtcag tcgtggctac caattcgatc ttggcggaca tggcaaaaga
     4321 ggtcggaaca gatcaaatag atatccacag tatcgtacca gtcggaacag atccgcatga
     4381 atatgaagta ctaccagaag acatcaaaaa ggcaagcgat gcagatgtta ttttatacaa
     4441 cggtttgaat cttgaaacag gtaacagctg gttcgataac ttgatggaaa cggctaaaaa
     4501 agaagaagga aaagattatt ttgcagttag caaaaatgta gaacctcttt atttaactag
     4561 cggtgaagaa catacaaaag cagatcccca tgcttggcta gacctatcta acggaataaa
     4621 atatgtggag gaaatcgcac gtatattctc tgaaaaagat gcagaaaatg cgacactcta
     4681 taaaaaaaat gcagaagcat atgtggaaaa actaaaagaa ttagatacac aagcgaagga
     4741 aagttttgct tctatcgaag agaacaaaaa attattagta acaagtgaag gggcattcaa
     4801 gtatttttca cgagcatatg atttgccagc agcttatata tgggagatca atacagaaag
     4861 tcaaggtacg cctgatcaaa tgaaagcgat cattgatcag ataagagcaa aagaagtacc
     4921 ggttttattc gtggaaacca gtgtagactc aagaagcatg gaacgggtag caaaagaaac
     4981 gggtttgaaa atctatgata aactgttcac tgattccata gcaaaagaag gagaacaagg
     5041 agattcttac tatcagatga tgaaatggaa tattgaaacg atccatgaag gtttgtcaca
     5101 aacaaaagaa agctgaaaag tgcaaggtct agcttgagga gcagaccttg cattttttat
     5161 aagagatatg aaaaaaaatt atgctgtttt tcgtcgtaca gatacctttt ttgaagaaat
     5221 tccgttatag ttagagtaag gaaggaaggt gtggctatga attttacacg taaagataaa
     5281 tatacgatta aagaagaagt ctttcgattg atcagccagc aggaaaatac cgacaaggat
     5341 caagaccttc aatttcttga agcagaatta gctaaattat tcatagcaaa tcaaaatgag
     5401 acaacagaac cagatgaaga gctgtacata ggcaaatggt tgtttgatga atttcagcct
     5461 ttagatgaaa ataccggtat cggttatttt gtgttccttt ctccagtcgg cttcacacct
     5521 ggcgtattag ttaaacaaaa cgacgaaata accgtcaaag catggcctgc attattttca
     5581 gtcatcaaag cagaaggagt aatcaaaaag atccagtcgg cctattataa aagtcaattt
     5641 attgccaaca gcaaattaat gaatatgttt tataatcgtc tagtagaaat tgctaggcct
     5701 gggaaaatcc cgcaaacatt cacgtacaca cagcaaaaaa atctctatga agaagtattg
     5761 tattcccgcg atctgtctga gttttcgcca tgggaagagg taaggctcca tgaactgtta
     5821 agtacaaaac cagtttcatt gaattttttt aatgcattag ataacaaaag aaaattaatt
     5881 tcaacaaatt attgggaagt atttgctgca agaaaaaaat ggatggaaga aaagaagatt
     5941 gccgatcagc agatccagtt tgtccagctg aaaaaagaaa aactatatta tgggttatac
     6001 agccgttaac agcaagcgag gaagaccatg acattaaaag aagaaatcat acaagaaagc
     6061 aaacgattag gaattgacaa aatcgggttc accacggcgg aaccttttga ttcattaaaa
     6121 gaatcgttgg aagaacaaaa agcagctggc catacttctg gctttgaaca tccaaatatc
     6181 gatgagcgtc tttatccaga aatgacattt gatcagccga aatcgatcat ttccattgca
     6241 cttgcgtatc ctactaggat ccatgaagaa gtgcctaggg atgaaaaacg cgggcagttt
     6301 gctcgtgctt cttgggggat cgattaccat gatatactca gagaccgttt gaaccgattg
     6361 atcgaattca tccaaacaag agcagaaaaa tggcaaaaag aagaggaatg gcgtctagct
     6421 ccgcaagtag atactggaga actgatcgat gtcgctgtag cacagcgagc tggtttagga
     6481 tttattggac gaaacggatt gttgatcact gaagagttcg gttcatttgt ctatcttggt
     6541 gagatcgtta ctaatattgt atttgagcct gataaaccag gagaatttgg ttgcggtgac
     6601 tgtatgcgct gcgtgactgc ttgtccgaca aaagcattgc ttggtgacgg aagaatgaat
     6661 gcccagcgat gtttgtctta tcaaactcag acaaaaggga tgatgccgga agaatatcgt
     6721 aaaaaaatgc gaaatgtcat ttatggatgt gatatctgcc agttagtctg cccttacaat
     6781 caaggaaaag attttcattt tcatgaagag atggaaccaa aagtagacga aatatatcca
     6841 aaattaaagc caatgctttc gatgtctaat aaagacttca aacagcaatt tgggcattta
     6901 gctggttcat ggcgtgggaa gaaacctttg cagagaaacg cattgattgc tttagctaat
     6961 ctaggtgatc gaacggcatt gcctgagatt tcagaatgtt taacagatgt ccgccctgtc
     7021 atcagaggaa cagcagcttg ggctattgga cgtctaggaa caaaagaacc ggaaaaatgg
     7081 cttgatgtat tagatgaaat attagctaaa gaaacagaag aagtcgttat aaacgaattg
     7141 gaacatgccc agaaattatt gcggagaaag ttaaaataaa aaaatagtta acattatt
//
LOCUS       DAZKMK010000117         6828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000117 DAZKMK010000000
VERSION     DAZKMK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.120"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..495
                     /locus_tag="ACG8V1_002572"
     CDS             <1..495
                     /locus_tag="ACG8V1_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HFQ0330608.1"
                     /translation="IETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSH
                     KSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRG
                     ELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSY
                     LKMN"
     gene            498..1394
                     /locus_tag="ACG8V1_002573"
     CDS             498..1394
                     /locus_tag="ACG8V1_002573"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0330609.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1444..1833
                     /locus_tag="ACG8V1_002574"
     CDS             1444..1833
                     /locus_tag="ACG8V1_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0330610.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1820..3319
                     /locus_tag="ACG8V1_002575"
                     /pseudo
     CDS             1820..3319
                     /locus_tag="ACG8V1_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4067..5893
                     /gene="ltrA"
                     /locus_tag="ACG8V1_002576"
     CDS             4067..5893
                     /gene="ltrA"
                     /locus_tag="ACG8V1_002576"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0330611.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            6006..6828
                     /locus_tag="ACG8V1_002577"
                     /pseudo
     CDS             6006..6828
                     /locus_tag="ACG8V1_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         2378 a         1113 c         1464 g         1873 t
ORIGIN      
        1 attgaaacat gtgtaaacga aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa
       61 gaaatacaag ataaggcgaa ggtagagctt gaaattgaag atttaaaaaa acaagtagaa
      121 aagctatctc ataagagtta taaagatgtt tatgatgagt attcaaaaga gacagatatt
      181 tatagggaac tgaaacgtga tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat
      241 agtgttgaca aaattgactt tggcaatggt ataaaagtta cagacgaaat tgatgaacac
      301 cgcggagaat tgattaagaa tttagtgacc aatcgggcta agattcaaac aataattaat
      361 gaaatgtcag atgaaattga tagttataga gcagagctga ataaatcaga ttggaaagtt
      421 aactataatg aggtttcaaa gaggtatcaa agaggtatca aaaacttagt gaagagttat
      481 ctcaagatga attaaaaatg ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa
      541 ctgaagagcg attggaagaa attattcaac aagaaataaa gcttaaagat taccaagttg
      601 aggttgactc agcaatgtca agaatgaata aatcatatca aaatttctat tctgagagaa
      661 gtagttcctc gcgcgaaatt ttttctgata ttacaggaat agaggctact attcaaccaa
      721 atagggattt caataattat gttaaagagc tcagagcttg tattaataag gaggacactt
      781 ttgacagtga gtttggatct cttattgata agctgacaaa aaattccatt actaatcaag
      841 atatatataa tgacatactt gatataagag ttagtgggga aagtagtatt ttttcagata
      901 aaaaattaat caattctata agaaaattga atgatgcaca agttcttgaa ttacaaaccc
      961 ttaagccaaa tgatttagta gttatccgac ttgaactaaa tggaaaaaaa gttagtttat
     1021 cgaatgctag tgctggtcaa aagacatctg caattctttc tatgattttg gcgtatggaa
     1081 attcaccact catattggat cagcctgaag atgatttgga tagtcaatta attaataact
     1141 taatcgtaag aagtatcatt acaaaaaagg aaaacagaca aattttaatt gtaactcata
     1201 atgcaaatat cccagttaat ggtgattcag aatggttgat tagcatggga gattcaaaag
     1261 aaatatcggc tgatcagtgc gggtcggtag atgaacccaa tatcaagcca agaatatgta
     1321 ctgtgatgga aggtggcgag gatgctttta caaatagagc gaaacgctat ggttttaaac
     1381 aaattgtaga ataacactag agggtgagac atattattat gtccgccctt tttaggagga
     1441 aaaatgaaaa aaataaaaag ctataccagt atctgggcag ttgaaaaagt catttacgca
     1501 atcaatgatt ttcagttgcc ttttcctgtg acttttaacc aaatggcttg gttcgtttta
     1561 tctttactgt ttgtcatcgt atttgcccat gtgcctccac tatctatgat agaggaggtc
     1621 tttttaaaat acttaggcat accggtggct gtcacttggt ttatgtcgca aaagacgttt
     1681 gatggcaaga aaccactggg atttttacga tcctttatca gctatcactt gcgatttaag
     1741 gtgacttttg caggaaagaa agtcaaagaa cagaagaaac gatgggatga acccagtact
     1801 cttgtaagga gtgtgaacta tgtatccgat taagtatatt gaaaataatc tcgtctttaa
     1861 ccaagaaggc gagtgttttg cctactatga attagtgcct tacaattact cctttttgtc
     1921 acccgaacag aaatttcaag tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga
     1981 aggcaagatc catgcgttac aaattgccac agaaagtagt attcgcgcca cacaagaacg
     2041 atcaaaaaaa gaaattaccg gtagactcaa agaagtagcg aaacaacgaa ttgatataca
     2101 gacagatgca ttagtatcca tgattggcga tagtcagatt gattatcgct tttttattgg
     2161 ctttaaacta attgcgacag atgaagaagt caatctaaag agtctcaaga aatctttctt
     2221 ttctgggctt caagaatttg tctatggagt gaatcatcat ttgatgggcg attttgtttc
     2281 cttatccaat gaagaaattc gctgctattc taagcttgaa aaattaatgg aaagcaaatt
     2341 ggctcgaaga tttaaagtca ggcgagtcac accgagtgac ctgacgtatt tgattgaaca
     2401 tatttatggc gaaaaaggga taccgtttga agaatacgaa tttcagctac caaagaaaaa
     2461 gctaaaatca gaaacactgg tgaaacgtta tgacttatta cgtccaaatc gttgtttaat
     2521 tgaagaaaaa ccaaggtgct tacggatgga acatgagaat catgagtcgt acgtagcgta
     2581 tttaacaatt aatacaattg tgggggagat ggaatttcct tcctcagaac ttttctatta
     2641 ccagcaacaa caatttacat tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa
     2701 taaaaaggct ttagctaccg ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca
     2761 tgcttaccaa tctgacaatg aaacgaattc caatgtctta gatgctttgg attccgtgga
     2821 tgaattggaa accaccttgg atcagtcaaa agaatctatg tacaaactga gttatgtggt
     2881 tcgagtgagt gccgaatcag tggatgaact aaagcgacgc tgtgatgaag tgctcgattt
     2941 ttatgatgat accaatgtaa aactagtccg gccctttggc gatatgatgg ggctacatga
     3001 agaattctta cccttaagta aacgatatat gaacgactat attcagtatg ttacgtctga
     3061 ttttttggcg gggcttggtt ttggggctac tcagatgcta ggagaattag aagggattta
     3121 ctttggctac aatgtggata caggtcgtaa tgtttacctg aaaccggcat tggcttcaca
     3181 aggagtcaag ggttcagtaa ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg
     3241 aggtaaatcc tttagtaaca atctcttagt ctactatgct gttttgttcg gtggtcaagc
     3301 tgtgattgtc gatccaaaag ggggtgcgaa acgttcctac ttgaaaagag taggcacagc
     3361 acttcattaa atcaatattt aatgtgtgtt ggagaactaa acacacgaat agtcgaatga
     3421 cggggtaacg ccctgaaagg ctactttaat actctgactg gcagaaactt gagtaaacaa
     3481 ggctctgtca gaagctcggt gaagtcggca gacagacacc gtaacagata atgctgacga
     3541 aagattccca gaggtgggcg ttgtgtccta tatgccggaa gtctataaaa tatctatggt
     3601 tagaatgtag atgagaggtc tactgacgaa tccttgaatg tacaggtcta tatcggggct
     3661 ggatagaaat gtccagtgac acaaacagtg tcgctactgt gggtgagtaa gactccgcat
     3721 ttatgaaaat ccatataacg ttaaaggcgt tatcaaggta gcaggcttat agaggaaacc
     3781 taaggatata tgtacagata gtgtgtttgg aacgttgaaa gctgacaaca tggagatttt
     3841 actgtctgtg aagtgttcac atcagaaagt tattgtgaga ttagcaatga tataaggtgt
     3901 gttaatgtca gtgagagtgg tggcacagta cctatgaaac cgtgataaca agcggtggag
     3961 ggatagccac tagtcatatg aagaatagaa ttactaagaa aaatggtatg ggttcgagta
     4021 agactaagag atgtacttgc tcctgcaaac aaggagggta cagaccatgc taaagaaaaa
     4081 caagctccgc tataacgagt attatgatat gcaacatatt tatgacgagt tatacgcaca
     4141 gagcaagaac ggtaataact tttataagtt acttgaaatt attggttcgg aacaaaatat
     4201 ttgtttagct taccgtaatc tgaaatcaaa cagtggcagt aaaacggctg gaacggatgg
     4261 aatgacgata gacgatataa aacaactcag caatgctgag attgttgcta ccgttcggga
     4321 aagccttagc aactaccgcc ccaaatcagt cagacgtgtt ttcattccaa aagctgggag
     4381 cgataaaatg cgtccattgg ggattccgtg tatctgggac aggctggtac aacaatgtat
     4441 cctgcaagtc ctagagccga tatgcgaacc aaagttccat aatcattcct atggattccg
     4501 tgcgaatcga agtgcccacc atgcagtcag cagagtaacg acactgataa atttatcaaa
     4561 atatcattac tgtgttgacg tagacattaa aggatttttc gataatgtca atcatggaaa
     4621 actattaaaa caaatctgga cacttggtat tcgggataaa agattgattt gcattatcag
     4681 caaaatgctg aaagccgaaa ttgacggcga gggtgtccca gaaaaaggaa cgccacaagg
     4741 cggtctgtta agtccactgc tctccctcat agtccttaat gaattagact ggtgggtaag
     4801 cagtcagtgg gaaacattcc agccgaagaa ccgcagtaaa aatgggtggc ttcaatacgc
     4861 aaagaaatat accaaactga aaagcggttt tattgtacgt tacgctgatg attttaaaat
     4921 catgtgttca acctatggag aagcacaaag attttaccac agtaccgtag attttcttaa
     4981 taaaaggctg aaattagaaa tcagtccaga gaaatcaaag gtagtaaatc tgaagaaaaa
     5041 ttcatcagat tttctggggt tcaaaatcaa agtcattcca aaaggaagaa cgaaacacgg
     5101 ttatgttgcc aaaacagata tgaaccaaaa agcattaaag aaagcgaaaa caaatctaaa
     5161 gttaaaggta aaagatatag ccagacatac gactggcttc aatatcagca gatataatct
     5221 gacagttatc ggtatgcaga attactactg catagcaact aacgtatata acaacctgac
     5281 agaagtaagt tacgctctct taccgaccat acgaatccgt ttaaggaaca ttgcaaaatc
     5341 agttccattt gaaagtacaa gttcagaatt tcagagcagg acaaaaggaa tcagaccaaa
     5401 aacaaaaata gtgatgattg cggataatcc gctattgccg attcaaggag tacaacacaa
     5461 aaatcctatg aatttctcgc aagatatttg taattttaca aagcaaggga gaaacaaggt
     5521 gcatgaggac gttgtagttg taacaaaaga agaaatccga gcgttgttgg aaaatgaaaa
     5581 ccctgccgat agcgtagagt ttaacgacaa tcgtatttcg gcatatatcg cacaacaagg
     5641 caactgctat gtaatgaatc gtcgaggaac tccgtccact ctgatatgta ttcacaaatc
     5701 agacaaagga gataaccttg acagatattc taatctggca tttgtggaaa ttcccattgc
     5761 aaaggcaatc ctaacagaat cagcagacga agcaaaatca ctgttaaaag gttatgttct
     5821 taatagtcag cagaagaaaa aactgaacag aatacggacg aattacggat accagacaat
     5881 aactgggaaa taacttataa atctatttgg atatgatgga acgccgtatg aagcgaaagt
     5941 ttcatgtacg gtgtgaagcg ggggaaaact tggagataac atcaaaaagt tacctatcgc
     6001 tatagcgagg agggtggaaa gaaaccttgc cagaaattgc ggatgaaatc agtatcctca
     6061 acttgacgag taagtcagaa aatcaaggtc tgcttgatcc ttatgtgatt atgaagaaaa
     6121 agaaagactc ggaaagttta gcaattgata ttctgacttt tttaacagga atttcaagtc
     6181 gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact caaagtgatc
     6241 aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca atcgcggaaa
     6301 atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg cttttctcag
     6361 acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt caagtggcgg
     6421 atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg gaattgctta
     6481 gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat agtaaccgag
     6541 aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg gctcaaggaa
     6601 aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg gtctatttcg
     6661 tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc gggttgaagt
     6721 ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggtttgg
     6781 atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaacg
//
LOCUS       DAZKMK010000118         6802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000118 DAZKMK010000000
VERSION     DAZKMK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.121"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..602)
                     /locus_tag="ACG8V1_002578"
     CDS             complement(<1..602)
                     /locus_tag="ACG8V1_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0330612.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYN"
     gene            complement(626..775)
                     /gene="rpmG"
                     /locus_tag="ACG8V1_002579"
     CDS             complement(626..775)
                     /gene="rpmG"
                     /locus_tag="ACG8V1_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0330613.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(796..1173)
                     /locus_tag="ACG8V1_002580"
     CDS             complement(796..1173)
                     /locus_tag="ACG8V1_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330614.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(1205..1384)
                     /gene="rpmF"
                     /locus_tag="ACG8V1_002581"
     CDS             complement(1205..1384)
                     /gene="rpmF"
                     /locus_tag="ACG8V1_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0330615.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(1399..1668)
                     /gene="rpsN"
                     /locus_tag="ACG8V1_002582"
     CDS             complement(1399..1668)
                     /gene="rpsN"
                     /locus_tag="ACG8V1_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0330616.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(2191..3957)
                     /locus_tag="ACG8V1_002583"
     CDS             complement(2191..3957)
                     /locus_tag="ACG8V1_002583"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330617.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(3917..5671)
                     /locus_tag="ACG8V1_002584"
     CDS             complement(3917..5671)
                     /locus_tag="ACG8V1_002584"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330618.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(5781..6350)
                     /locus_tag="ACG8V1_002585"
     CDS             complement(5781..6350)
                     /locus_tag="ACG8V1_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0330619.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(6368..>6802)
                     /locus_tag="ACG8V1_002586"
     CDS             complement(6368..>6802)
                     /locus_tag="ACG8V1_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0330620.1"
                     /translation="DYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFD
                     LSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHD
                     PEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         2191 a         1296 c          943 g         2372 t
ORIGIN      
        1 aaattataaa aatccttttt ttgaatcgtt tcactatgtg tgaacattcg aatatcggat
       61 aaagggttaa tacttttgat ttctctctgt aattttttta gtgcagataa attcaagttt
      121 tgttttctag acacaaaaat gcgatctgcc atgacaagtt gcttaagggc ttgtggttct
      181 ctttcaataa gttcaaaact gtcactatca ataacagtca ataaactatc gatatatact
      241 cttcctctta gttgaggcgc tgttaaaagt gtttgaatga ttggttgggg atctgctata
      301 cctgtagttt caatgatgat ttgtgaaatt ggttgttgat gttcttctat tgtttccagt
      361 aaagaaatca atattgctaa taaatcactg cgaagactgc aacacataca tccattattt
      421 agttgtaaca cccgttcctc gctatgaact aataatttgt gatcaatccc aacttcacca
      481 aactcatttt caattataat aatttcttcc ctggggtagg gtgagacttg caaggcttga
      541 ttgattagcg ttgtttttcc agcccctaaa aacccagaaa cgattgtaac tggtatgccc
      601 attttgtcac ctttttacca cttagctatt tcacttcttt aaaaattgcc cgtctgcgca
      661 attttggcga atactttttt aattctaacc gttcagggtt attccgttta tttttgctgg
      721 tgagatacag acgttctcca gtttcgacac attccaaaat aatattttga cgcataaatc
      781 ttcctccttc aatacttatc gatcttcaag atatgcttga atttgttgtg catcttcaaa
      841 agttaactcg ccagattcca attgtgaaac taaattttct ttcatatcaa caggtatctc
      901 ccaataacca tcgatacatt gaaataactc ctcgtcatac tcttcaaaaa ttggatagat
      961 tcttaaaaaa tcatcaaatg ataagccttg gtcctcatag taatctttga ctaatccatt
     1021 ttcaattgcc tgttgggtcg gtaaacccgt ttcagtaata attcctctct ctaccatcga
     1081 acgataaaaa gtgcatttat ctagcgattg ctgggtcgtt tcaagataat actttaatcc
     1141 ttcttcaact aaaggtgggt aaataatttc cattttgatt ccctcctaaa agttatatat
     1201 taaactattt tgaagctcct ttaattactt tctttccatt atagtaccct tttaaagata
     1261 catgatggct acgtctataa tcaccattcg cttcgtcaaa tgaaatttct ggttttacta
     1321 cagtttgatg agttcttctt aaccgtttct tcgccttaga tgtttttctt gctggtactg
     1381 ccatgatatt tcctcctttt accaacttgc tttttttact ccaggaatca acccttgatg
     1441 ggcaagttct ctaaacttaa tacgggacat accgaacttt cgcatatacc ctcttggacg
     1501 accatccgtc tgatcacgaa gcctcagacg attcggattt gaatctcttg gcaatttagc
     1561 gagagcggtg cgatctcctg cggcttttaa ttcctgacgt ttatctgcat acttttcaat
     1621 catcgccatt tgtttctttg ctttagcaat tttcgacttt tttgccatat acctttatac
     1681 tccttttatt ttgatttaga cacctcaaat cgtaatcgtt acgttttgac actgatactt
     1741 taccaacaag agttgctctt gtcaacaata aataacatga aataaaactc atatgaatag
     1801 ataactattt gaaaacgaat ttatcggtac atagagataa tcaaatcatt taaaagaata
     1861 tgcagtttag gctctgaaaa ataagaattc acttttatta gtccaatcaa acacaaatac
     1921 cgttttaagt agcttttcct ctacttaaaa cggcaatatg aaagaaacta taacttttta
     1981 ttcaatcttt tatgtttaac tggtttatcg ccaatttttt aaaactttga ttattcctta
     2041 tattataaga atagacatta agtgaactaa ttcaatcata ctgattcttt cgtgtattca
     2101 aaattagatg ctgtttaata aaattattct ttttgaaaaa gaattataac tatatcccaa
     2161 aaattgtagg ttaatttaaa gcctatttca tcatcgtgat tgagcttctt tttcatagac
     2221 gaatttctca gatagtttag ttttctttat gagatttttg tatatttctg accttcgata
     2281 caactcatta tgagtacctt gatcaactag ttttccttga tccatcacta caattttatt
     2341 taccttttca atagacttca agcgatgcga tataattagt actgtcttat cttgtattaa
     2401 acgatttaaa ctttcctgaa tacgtttttc attttccgca tctaaactgg cagtaatttc
     2461 atctaaaatt aaaattggcg catttttcaa aaaagcccgt gctatggata ggcgttgtcg
     2521 ctctcctcca gaaagtgaac ttccgttttc tccgaccaat gtatgataac cgtcaggtag
     2581 tttttcaata aagtcttcgc agttagctaa acgagctgct tttttcactt cctcatctga
     2641 cgcttgacta tttccaatac gtatattttc caaaatactt gtattaaaca aagttacctc
     2701 ttgaaaaaca atagcgattt tgctaaacaa agaagctgga gatacacgtt taatatcata
     2761 cccatcaatt aaaatacagc cttcatcata atcatacagt ctagacacta atttcaagat
     2821 actagttttc ccggaaccac tagcacctac taacgcagtc acttctcctt gtttagccgt
     2881 aaaagagata tgatctaaaa ttggggtatt gttatcatat gagaaagaga catctcgcaa
     2941 ctcaatgtca aaagatttca gaggactatc tgatccctct tggatactgg tttccttcat
     3001 agctctaatt cgctgtattt taggagctaa ataaaatatt tcaagcactg cttctttcat
     3061 agaatcgaag gaatccttta tttttattgc ggctagtaag taaccaacga cgtagagtat
     3121 agtcacttca cccgttaaca gtaagtgaac acctactaga atgactactg ctaaactaac
     3181 atatgagaaa atagaggata aggccatgac tgaaaaagta cttaattcta cctttaaatg
     3241 aatcctttca ctctcttcca tttttttgaa aagtctgtct tgaacttttt tagatagatt
     3301 aaagctatta atctcttgct gcaattctat agtttcttga aattcttccg agttttctct
     3361 caaagtatcg taatatttag tattggcttt cgtttgggat ttctttgata acaagattaa
     3421 gacaaaacta aataacgttg gcaaaataac agctaatccc attttgacat taccaaccaa
     3481 aagcatcact gaaataaaag ggaaaaacag agccataccg ccggctttag gtattgcatg
     3541 actcatcgca tgctcaatac cttcaacgtc agacatgata gtttgagata aatctgataa
     3601 attatgtttt gaaaaatagg ataatggtaa atttgacaaa tcgtctgcaa tttgcactct
     3661 taaatgcgca ctttctttat aagtactatt atataagttt tcatactccc tatctaacaa
     3721 ccaatacatg aaaagcaaag taacaaacga tacggcaaaa taaagccaac ggggctttgt
     3781 attttctaaa actaattcgt ctatcagcat cattataatg aatgctggta ccatattaat
     3841 gcagtataca aaaaaactag aaatagaagc tttagttaaa gcttctgcgc cctgatcact
     3901 taaagcaaat ttgtttttat aaaaactttt cattgtcaag cctccaatca tttgcattct
     3961 cataattagt gaccaattgt ttatacactt gatgtttagc cattaattca ttatgatttc
     4021 cacgttctac tattttccct tctgacaaga caagaatttc atctacagat ttaatgctgg
     4081 ataatcgatg agctatcata ataactgttt tttctttcat cagatgttga aaagcctttt
     4141 gtagttcata ctcattatct ggatcaatag atgcactggc ttcatccata ataacaatct
     4201 ttgcatcttt taaaattgct ctggcaatag ctatacgttg tttttctcct cctgacagat
     4261 aaactccttt actaccaatt aaggtatgct ctctttcggg aaatttttct aaaatcgatt
     4321 cgcaaccggc taatcttagc gcgtcaaata ctttacttgc tggcgcttga ggatttgcta
     4381 tagaaacgtt atcgtaaata ctctgattga atagtttcgt atcttgaaag acaaaagcaa
     4441 ttgtgttaat aattgtgtct tttgaatagt gatttaaagg cttaccacca attttaatct
     4501 gtcccttatc cacattgtag aaacctgaaa ttagtttagc tatcgtactc tttccgcttc
     4561 ctgattttcc tactaatgca taaattcttt tttgttctaa aactaagttg aaattttcta
     4621 aaacacactc ttctttgtat gaaaagctga cattttcaaa tgcaatgtca caatttttaa
     4681 tctgtgtctc gttcccatat tttagttggt cttgcttcat ttctaaataa agggagtcta
     4741 gtgtttcgac tgcatatctt gcttcaaaca agtgcatact tacatacatt attctcatga
     4801 aagaaacaaa aataattcca cttaaaaaaa acaacatcaa caagtcaaca gctaatatct
     4861 tgggagtggt aagagaagtt ataaaaaagg ttataggaac tatcaaaatg caaactatgc
     4921 caaaaaatag ccattgaaaa agaacaaaag gtgttttccc actcaaagaa tacttatatg
     4981 caaacttagc ataatcgtta attgctttat tcaaggcttt aaatgatgtt actttagcac
     5041 caaatatctt tataacttga attccgcgga tatattctac cgtttcagag cttaacacat
     5101 ctaacgattc ttgatatttt tgaataaaat ttgtattctc tgtcattttc tttaacaaga
     5161 atacaccaat tactaacatg gcgattagaa ccaatccaat tttaaagtta atgaaaaagc
     5221 ctaaggcaac tgataaaaca ggtgttacta tggcttgagc actatctgga atcaagtgag
     5281 caattgccat atgtgtttta gccgcgttgt catcaattgt ctttcgaata tatcccgaag
     5341 aatttaaatc aaaaaaacga aaacttgctt ctgttaaacc gctaattccc ttttttctca
     5401 aattagtctc taagcgaaac ccaaggatat gagaaaataa gccagaaaaa atataaaata
     5461 aagctcctaa agttaaccaa gcaacagttt gaaccgcata actcttagct aaatattcat
     5521 ctcctacaat aattagagac tgaagaaatt tgtaaatact gtaatatcca tagactgtta
     5581 atacgacaga aaaatcagaa aataaagtgg ctaaccatcc gtagtatttc attttaggga
     5641 cataactaaa taattttttg tatactttca ttttttcctc ccccgcattc aatttcttct
     5701 cttattcgtt tacaactgtt aatgctttcc gtaatagcgt aaattagaga tgtacaaaca
     5761 gataaaaggt aaactacgtt ctattttttg tattttttta agacttttct tccaatcaat
     5821 acaccaatca tagataaact tgctacaact aacactgaaa tgattcccca tatacctacc
     5881 gcatagtgga tcattgcatc cattgttgca gcagattgtc ctttatctat ccaatcttga
     5941 cgaaaacgat ctgtaaagaa ccatatagga atccactgtc cggcagcact gcctaattgc
     6001 attagagccc aagacgcaac ttgaggaatt gctcgatcat atccaaatac agatagaata
     6061 atatcagcta agatcgccat actaatcgaa ctgataaacc aaggtaagta cgccccgccc
     6121 attaacgtaa aaaataacaa taaaacaatt gtgtaaatag taaaaattcc ttttctaggg
     6181 tagttatagc taatataaac aaatatgatt ccagataata atccaaaaat cccgggggat
     6241 atgctgtgac caaacgcccc accaatacta ctaaccatta ttgaaaaaaa gtaaattact
     6301 agggaaataa ctgtagctaa accaataaaa atataatctt ttgttttcat atatattctc
     6361 ctcaattcta tttcgttatg tcattaaaaa tctgttctat caaagttgaa tcttctactc
     6421 taagttttat atcttttttt attccaccat tttttaaata aataattcta ttgcatgttt
     6481 tataaagaaa ttccggatca tgagtagcta taactactgc acccttttct tttaagtctt
     6541 taataaggtc agcaatcctt tccatattaa tataatccaa cccacttgta ggttcatcaa
     6601 atagaaataa attagttttt gacaatgaag ctatagaaac caataatctt tgtttttctc
     6661 caccggataa atcaaacggt aattgatctt tcattttata aagttccatt ttttttaaaa
     6721 ttttttcttg ctgatcttta ctaactattt cattccccag ggctaactcg ctcctcactg
     6781 attctgtgaa aaattggtaa tc
//
LOCUS       DAZKMK010000119         6794 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000119 DAZKMK010000000
VERSION     DAZKMK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.122"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG8V1_002587"
     CDS             complement(<1..434)
                     /locus_tag="ACG8V1_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330621.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG8V1_002588"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG8V1_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0330622.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1036..2190
                     /locus_tag="ACG8V1_002589"
     CDS             1036..2190
                     /locus_tag="ACG8V1_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0330623.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2163..2606
                     /locus_tag="ACG8V1_002590"
     CDS             2163..2606
                     /locus_tag="ACG8V1_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330624.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3740..4708
                     /locus_tag="ACG8V1_002591"
     CDS             3740..4708
                     /locus_tag="ACG8V1_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0330625.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            4718..5206
                     /locus_tag="ACG8V1_002592"
     CDS             4718..5206
                     /locus_tag="ACG8V1_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0330626.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            5502..5783
                     /locus_tag="ACG8V1_002593"
     CDS             5502..5783
                     /locus_tag="ACG8V1_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330627.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            5785..6201
                     /locus_tag="ACG8V1_002594"
     CDS             5785..6201
                     /locus_tag="ACG8V1_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330628.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2458 a         1161 c         1108 g         2067 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttgtata gactgattgt aacatggaac
      901 aaaataaaaa ggcaatacgc cttagttgtc ggtcttcgag ttaccaacgg taaccctcat
      961 accgaacggc attcatcacc cacctataga ggatgggcga cttctgccta attctgttaa
     1021 atttaacaca aaggcgtgat tattttgaaa cccaaaaaga aaatttatct aggtctcctc
     1081 ttttccttga tgattggaat ttttagtata ggaattacaa atacctatgc ttcagaaaat
     1141 tctaatttca atatcaactc ccaaccaatt gaagaacaag caattaattt tgcgaaaact
     1201 gattttgaac actccataac ttcaattctt ttaatggaaa ataacaccga aactgatatt
     1261 aactatactc ttggttcgcc atttaaaatg ctcgatggag aaaatacggc aaatagttct
     1321 attttctttc cagtattttc agacgctgat aatacgatta aatatgttta taccgtcaaa
     1381 aaaaatacaa atggagaata ttcaggaagt atttcacaat ttttagcaaa agaacttcaa
     1441 aatctagtag atagtaatat aacagataca accttatttt ttcaagataa tagcatttat
     1501 tattccgata atggacaaat taaactactt tggaaatcac ctttaagtac agaaaaaaat
     1561 ggtgtttctt cccttacaga aaaaaatgat cttatatccg taaatataaa aaataaaatt
     1621 aaaaattttt ctaaaaaaca aaattttcct gcggttaaac gtgtaaaaag tgtctcctcc
     1681 ggagataact ttattatgat taattggcgt atagcagaaa cccaaacaag ttttccgtgg
     1741 tgcgctgctt acgtagaagc tgctatctta tgtaacaaaa cagatgtacg tgctacttca
     1801 gctagagcca ttatgcatta tacctatcct aatttaaaca acagtcaaat tgaatcaaaa
     1861 agtcttaatc aagatcaaat aataaaatat gcaaattact gtggtgttta tcctaaaaaa
     1921 atagcacgaa tactaaacct ttcagaggtc caaacacaac taagaaaagg aaatgccatt
     1981 tatatgggaa ctgctggtta tggaaattat agtaacagcc gtcatgcact tgcattattt
     2041 gggtgggtca atcaaggtgg ttatcaaact tactatgtgt ggaatccttg gtataactac
     2101 ccaactgtaa taagtgcaaa tgcaggacca gttacaattc ctgtacctgg aggtggtttt
     2161 acttgggaca ataccattac aaattggtaa ataaactcat tgctttagtt atgggtctag
     2221 tgttagttct tttcttaact agttgttcaa aaaaacaaga aatacaaaag actgatctga
     2281 ttccttttct aggaacttgg caaactaaag atcattcaga acgtctagta atcactaatt
     2341 taaatacttc aaaaaatata gaacaatata ccatcttagt aaaagagaac aatcataaaa
     2401 ttcaaatcat tgataaaaat ttaaatataa atgaacaagt tttcatttac aaagcaacaa
     2461 ataatttact aacactgcgc tcgaataaaa aacaattaga ttacactttt agtttggatt
     2521 ctccaaaaaa gctcgtattt attttgacaa cgaatgatgg gacagaaggg acagctaaac
     2581 caattgaatt ttttaaagct gattgatgaa tagttgtttg atattacaaa acaataattt
     2641 caccgaaggt gaaacaagcg aaactttaca aaaagtggct agcatttgct agcctttttt
     2701 gtattgtgga gcgtgggttt tgagggggat tcaaggggga gcttgcgccc ctttgacaag
     2761 cggttatttg caccaaatac gtaggtattt ggctagcaaa tatagagctt gccaagccct
     2821 ttagtgtgtt ttgtagaaag ggaccttata gtcactttca cattacaaac gatacgtcct
     2881 atggacgtat caaaagggtg ggacttatga gcaaattaaa gcaaaatttg gctagcaatc
     2941 cttctaaaaa atacacctat tgatcagagt caaaataaat cagttttcga gtgagcgaat
     3001 ccaaatttgc aactctgaag ctttgcaaat gagtgtgccg gcttccgtaa aagtctaggt
     3061 aacattagtc gatatataca tagggctcta atttgcgttt taaggcgttt taaacgttct
     3121 ggtatgtaat tataccaaag cgactttaaa acgccttaaa acgaatttta gaggttctgt
     3181 agaacgtttt agggttgtta gggcttgccc tgaccgcctg taaggcgctt taaaaacaag
     3241 ataagggatt ttatctagac ttgtcttaaa acgccgtaga tgagcgctca gagcttctaa
     3301 aaggactcct tacgaactgt ttttaagcaa aggagccttt aggcgactgc gcagtttaat
     3361 gtgagagcgt agcgatcaca cctttaaagc gaccgtaggg agcagattcc cagtttagga
     3421 aatttcgcga acctgtttgg agaaaaaaag taggaaaatt ttaggcggga aattttgccc
     3481 ccctctttta taatctcttt tataacctct ttttaaacct ctttaccgtc ggctgtatcc
     3541 ttactctccc aaggtgtgca gaatctttta ggggtctttt tgtctgtttt taggggtctt
     3601 tttgtctgtt tttaggggtc tttttgtctg tttttagggg tctttttgtc tgtttttagg
     3661 ggtctttaat tagggggttg acccccatta gaaagctctt tataattttg ttaagtagtt
     3721 aatcttgaag gaggatttta tggcaaatga aattgtgaaa taccaccatg aattaaatac
     3781 gattccttta cgcaaattta cgtcagtaga aatgaattta ttcttctcta taatatctag
     3841 aatgcgtgat gtaggcgata aaaaagtaca atttacattc gaacaattaa aagatttaag
     3901 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     3961 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgatttgt
     4021 tttatttaat caatttaaaa tcgatggaaa agctgatatt ccttttgctg aaatacaagt
     4081 ccatgagaaa gccttaccct tacttaataa tcttgaagaa tgggttcgat attctctcca
     4141 acaattcaat gaattagaaa gcagttattc caaaaccatg tttagattat taaaacaata
     4201 cagaaccaaa ggaattgcca atttttcaaa agaagacttt catgaattac ttgatatccc
     4261 taaaagttat agagaaagtg atataaataa aaaaattata aagcctattc gccaagaact
     4321 aaccgcaatc ttcaaaggtt tgacaatcca aaagagatac ggaaaaggac ggggaaagcc
     4381 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     4441 acatgctcca accaaagaag aacaaaataa agctttggaa ctagctaatc aacaggttca
     4501 atctgaaaaa gaacctactt cattacaaga aaaaatcaaa gaacaaaaac gaaaattaga
     4561 acttttacaa cagttagcag aattagaaag aaaacaaaat accccaagta agaaaattaa
     4621 caaacaaata gcagataaca attccgatat taccccagaa tttaaagaaa acttactaga
     4681 taatctccaa aacttattta aagaataagg ggtttttatg acagataaaa ctatcaaaga
     4741 attagcagat attctaggtg tttcaaaaga taaagtgaag tatcaactga ggaaattacc
     4801 tagtaatttt acctacaaaa aaggaaatat tacctacctg aaaaaagaag cagtaacaac
     4861 aattacaaag gttttatcag gtaatgaatc gggtaactta cctagtgaat tacccgattc
     4921 attacctacc gattacctac tcaaggagat tgaaactaag aataatcata ttgaagaaaa
     4981 agataggcaa attgaaaaac ttcaaatagc tttagaaacg cagcaaaaat tactcgacca
     5041 gcaacaacaa ctcaccctac aagccaatca acaaatcaaa aaacttcacg aacaactcca
     5101 gctaacctat gcagaagaat cttcagaaaa agaacccctt tctgttactg aagaaactca
     5161 aaaaggacaa aacaaggaga aaaaatggtg gcaattctgg caataaccca acaattttta
     5221 ttcataaaaa ttgttgggtt actttctttt tttgctttta caagcatagc gacatgatct
     5281 cacgtcgcta ttttttaaag agcataagga aacgcggtat cactttctta cgaaaggtgc
     5341 taggtaggct gtgcctacca cgtttctaag aaaaaaattt gctatcactt tttcgtcatt
     5401 gtggatagct tatgtatagt gtttcggcaa aaaacagcta tacaaatact atatttgtat
     5461 gatatacata aattatacac aaatgcgaaa ggaattcttc tatggacatt ttagttcgga
     5521 atattgatcc aaaagttatt tcagaaatag atagacgttg ccaagaactc acaacaaaaa
     5581 ctggcaaaaa atggagccgt aatgcgtacc taaaagttct aatcgaaaac gattttgacc
     5641 aggcacttag gcattacaaa aaagatcaat tcgatttatt actcgaaaaa tttatcgata
     5701 tacaagcaaa aaatactgaa acattgaacg actatgttca tacaaacaac caactcattg
     5761 aaacacttat cggaggaata taacatgaga aaaacttctg ttcaaaaaca cgttaccctt
     5821 tatcccagtc acattgacgt tttagattcc atcatcgaaa aaaatccaga tatcaataac
     5881 tactcggaag ccattcgttt tctatgtatg agttatgaac ctaatactaa tacgacaact
     5941 accagcaacc aagcaaaact aaatgcgatg ggaaaagaaa tttctattat gtccgaaatc
     6001 attgcagaat ttgctgattt atatctaaaa gatactggtg ttcttatcgg tacaaattct
     6061 aaagtatatc aagaagtgaa agaaaaaatt gaaaatcaac tcaatccctc ccgacgaaat
     6121 caacccaaaa atcgtcaaaa agaaaccaat caaacattca cttctagaaa ttttgaccca
     6181 acaaactttc taaaaagatg aaaaagaaac cgaaaatttt ttctatttct cagtttcttt
     6241 tttagggggc agtcttccag caagatttgc aagttaccgg taacttgcac aaaaaaagaa
     6301 aaatccgctt gtgcgaatcc ttctttttgc attttggggc gctgccccaa tctccaaata
     6361 tcggttattt aatagcgtgg taactcaaaa aaatagaacg tactctgaaa taaaaaaaga
     6421 ctgctctaac gagcagccaa taaaaaataa tgagcgttta ttcttgaaaa agcagcgcgc
     6481 ttatgaagcg ctgccaaaaa tttaccatat tttatgaaaa ataacaagtt tctaaaaagg
     6541 aatcggttaa gattcacaaa gaatcgtaaa cagacaatag cggaaaatca agcttccgaa
     6601 cgtacctttt agctagacca atgaaaaatc acaaagtgag cccctgtatc ggttgataca
     6661 agggctactt ggcatactcg acattatgtc tactagctcg ataaataacg attatgttcc
     6721 caaaacaata ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt
     6781 ataggtgtat atca
//
LOCUS       DAZKMK010000120         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000120 DAZKMK010000000
VERSION     DAZKMK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.123"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8V1_002595"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8V1_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8V1_002596"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8V1_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0330629.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8V1_002597"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8V1_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8V1_002598"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8V1_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0330630.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8V1_002599"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8V1_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0330631.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8V1_002600"
     CDS             3582..4271
                     /locus_tag="ACG8V1_002600"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0330632.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8V1_002601"
     CDS             4285..5787
                     /locus_tag="ACG8V1_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0330633.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8V1_002602"
     CDS             5800..6276
                     /locus_tag="ACG8V1_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0330634.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8V1_002603"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8V1_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKMK010000121         6124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000121 DAZKMK010000000
VERSION     DAZKMK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.124"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG8V1_002604"
     CDS             <1..189
                     /locus_tag="ACG8V1_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0330635.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="ACG8V1_002605"
     CDS             complement(275..1369)
                     /locus_tag="ACG8V1_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0330636.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="ACG8V1_002606"
     CDS             1558..2319
                     /locus_tag="ACG8V1_002606"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0330637.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="ACG8V1_002607"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="ACG8V1_002607"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0330638.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3094..3849
                     /gene="fabI"
                     /locus_tag="ACG8V1_002608"
     CDS             3094..3849
                     /gene="fabI"
                     /locus_tag="ACG8V1_002608"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0330639.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3959..5011)
                     /locus_tag="ACG8V1_002609"
     CDS             complement(3959..5011)
                     /locus_tag="ACG8V1_002609"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0330640.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            5137..6057
                     /locus_tag="ACG8V1_002610"
     CDS             5137..6057
                     /locus_tag="ACG8V1_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0330641.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1880 a         1214 c         1094 g         1936 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
     3061 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
     3121 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
     3181 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
     3241 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
     3301 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
     3361 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
     3421 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
     3481 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
     3541 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
     3601 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
     3661 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
     3721 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
     3781 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
     3841 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     3901 tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt
     3961 attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca
     4021 attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa
     4081 gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac
     4141 cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc
     4201 ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg
     4261 gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta
     4321 cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt
     4381 cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac
     4441 cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc
     4501 cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg
     4561 gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc
     4621 ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa
     4681 cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc
     4741 gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta
     4801 gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt
     4861 aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac
     4921 gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac
     4981 caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat tttattttca
     5041 gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca
     5101 atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc
     5161 ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg
     5221 tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa
     5281 agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat
     5341 ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta
     5401 aagactttac atagtctttt aaatactatt tttcattata cccttggttt cttttttatc
     5461 tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt
     5521 gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt
     5581 tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg
     5641 attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt
     5701 actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat
     5761 atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag
     5821 atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa
     5881 ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc
     5941 tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa
     6001 gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt
     6061 ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt
     6121 tgtc
//
LOCUS       DAZKMK010000122         6066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000122 DAZKMK010000000
VERSION     DAZKMK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.125"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG8V1_002611"
     CDS             complement(254..541)
                     /locus_tag="ACG8V1_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0330642.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG8V1_002612"
     CDS             complement(561..785)
                     /locus_tag="ACG8V1_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0330643.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG8V1_002613"
     CDS             complement(1240..1410)
                     /locus_tag="ACG8V1_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330644.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG8V1_002614"
     CDS             complement(1425..1958)
                     /locus_tag="ACG8V1_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0330645.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG8V1_002615"
     CDS             complement(1979..2923)
                     /locus_tag="ACG8V1_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0330646.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACG8V1_002616"
     CDS             3560..3646
                     /locus_tag="ACG8V1_002616"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0330647.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACG8V1_002617"
     CDS             complement(3767..4456)
                     /locus_tag="ACG8V1_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330648.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="ACG8V1_002618"
     CDS             complement(4463..4741)
                     /locus_tag="ACG8V1_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330649.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="ACG8V1_002619"
     CDS             complement(4701..5615)
                     /locus_tag="ACG8V1_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0330650.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACG8V1_002620"
     CDS             complement(5597..5977)
                     /locus_tag="ACG8V1_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0330651.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1119 c         1021 g         2320 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DAZKMK010000123         5980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000123 DAZKMK010000000
VERSION     DAZKMK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.126"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..386)
                     /locus_tag="ACG8V1_002621"
     CDS             complement(<1..386)
                     /locus_tag="ACG8V1_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330652.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKIL"
     gene            complement(571..969)
                     /locus_tag="ACG8V1_002622"
     CDS             complement(571..969)
                     /locus_tag="ACG8V1_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0330653.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(966..1739)
                     /locus_tag="ACG8V1_002623"
     CDS             complement(966..1739)
                     /locus_tag="ACG8V1_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0330654.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1736..2419)
                     /locus_tag="ACG8V1_002624"
     CDS             complement(1736..2419)
                     /locus_tag="ACG8V1_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0330655.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2586..3473)
                     /locus_tag="ACG8V1_002625"
     CDS             complement(2586..3473)
                     /locus_tag="ACG8V1_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0330656.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3470..4339)
                     /locus_tag="ACG8V1_002626"
     CDS             complement(3470..4339)
                     /locus_tag="ACG8V1_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0330657.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4476..5762)
                     /locus_tag="ACG8V1_002627"
     CDS             complement(4476..5762)
                     /locus_tag="ACG8V1_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0330658.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1858 a         1318 c          978 g         1826 t
ORIGIN      
        1 agaatctttt gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc
       61 atttgttttt gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt
      121 aaaccttctg gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata
      181 taatctttta ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct
      241 tctgcttgat agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc
      301 agcaatgttt tattttcctg caattgaaca tatagaaatg tagatcgctc aatcagagta
      361 tcttttaaaa caactaaaaa attcattttt ctcccccttt tttcatcatt tttaaaatag
      421 catattttga aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca
      481 taagtattca cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg
      541 tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt
      601 ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc
      661 aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat
      721 gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac
      781 aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga
      841 acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac
      901 atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag
      961 atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca
     1021 tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc
     1081 attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt
     1141 gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta
     1201 tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg
     1261 tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat
     1321 cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc
     1381 ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct
     1441 actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca
     1501 atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa
     1561 aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg
     1621 cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc
     1681 aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt
     1741 ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca
     1801 ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa
     1861 gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa
     1921 tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag
     1981 ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata
     2041 ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata
     2101 tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc
     2161 cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc
     2221 ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc
     2281 cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg
     2341 caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt
     2401 ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa
     2461 agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca
     2521 cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg
     2581 tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat
     2641 acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt
     2701 gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc
     2761 aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc
     2821 gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt
     2881 agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga
     2941 aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg
     3001 attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc
     3061 gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt
     3121 tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc
     3181 accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac
     3241 aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc
     3301 acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat
     3361 aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct
     3421 tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt
     3481 ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg
     3541 aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt
     3601 gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa
     3661 aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat
     3721 ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt
     3781 cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag
     3841 tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc
     3901 agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt
     3961 gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa
     4021 agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa
     4081 taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa
     4141 aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa
     4201 atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa
     4261 taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga
     4321 ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa
     4381 gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga
     4441 tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact
     4501 tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga
     4561 gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt
     4621 tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct
     4681 tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc
     4741 gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct
     4801 gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct
     4861 tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat
     4921 tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact
     4981 gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct
     5041 tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc
     5101 gtgactgtct ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt
     5161 actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca
     5221 tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa
     5281 cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga
     5341 acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct
     5401 tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt
     5461 atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa
     5521 gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt
     5581 tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc
     5641 cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct
     5701 gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc
     5761 atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca
     5821 aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa
     5881 aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa
     5941 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc
//
LOCUS       DAZKMK010000124         5970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000124 DAZKMK010000000
VERSION     DAZKMK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.127"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..709
                     /locus_tag="ACG8V1_002628"
     CDS             <1..709
                     /locus_tag="ACG8V1_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFQ0330659.1"
                     /translation="PFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLS
                     TGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDH
                     SLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGD
                     GVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKK
                     VKRPISADSVLLEEDVE"
     gene            706..1854
                     /gene="neuC"
                     /locus_tag="ACG8V1_002629"
     CDS             706..1854
                     /gene="neuC"
                     /locus_tag="ACG8V1_002629"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0330660.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1827..2504
                     /locus_tag="ACG8V1_002630"
     CDS             1827..2504
                     /locus_tag="ACG8V1_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0330661.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2455..3636
                     /locus_tag="ACG8V1_002631"
     CDS             2455..3636
                     /locus_tag="ACG8V1_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0330662.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            3649..4626
                     /locus_tag="ACG8V1_002632"
     CDS             3649..4626
                     /locus_tag="ACG8V1_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0330663.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            4626..5534
                     /locus_tag="ACG8V1_002633"
     CDS             4626..5534
                     /locus_tag="ACG8V1_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFQ0330664.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            5535..>5970
                     /locus_tag="ACG8V1_002634"
     CDS             5535..>5970
                     /locus_tag="ACG8V1_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0330665.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSA"
BASE COUNT         2203 a          737 c         1140 g         1890 t
ORIGIN      
        1 tccttttgat gaagaaagtg cagactttct aaaacaaatt ggtgtacaag gatttaaaat
       61 tggctcagga gatttaacaa atttaccttt tcttagaaaa atagatatgt atgggttgcc
      121 agttttatta tctacaggta tggctgatat tcatgaggtc agtgaggctg tagattgttt
      181 catagatagt ccagttacta ttttacattg tacttcaaat tatccggcta gttctgaaga
      241 tattaattta ttggcgatta acacattaaa agaaatgttc gggatacccg ttgggtactc
      301 cgatcattca ttgggatatg atgttggtat ttgtgctgtt gcgatgggtg ctaaggtaat
      361 tgagaaacat tttaccttgg ataaggaact acctggtcca gatcataaag cttctttaga
      421 tcctcaggaa tttgcagatt ttgtaaagca tattagaaac actgaaatat ttttaggtga
      481 tggtgttaag cgagcaatgc cttctgaact tagtacaaaa gaagtatcaa gaaaaagcct
      541 agtggtcact gagaatttaa atgtgggaga tattcttaca gagaaatctc ttacaataaa
      601 acgtcctgga aatggaatag ctcctaagtt tttatatcaa tatattggca aaaaagttaa
      661 aagaccaata agtgcagata gtgttctgct tgaggaagat gttgaatgaa aaaaatactt
      721 atagtaagtg gaacacgagc tgattatgga atatataaac ctgttgctga gaatttacta
      781 caagataatt gggatgtatc atttgttgtt tctggaatgc atttatccca taaatatgga
      841 tttactaaag aattgatctt agctgacgga tttagaatta gtggagaagt tcaaagtttg
      901 tttttagaga acaataaggg aaacatggct cggtctgtgg cattagagat aaatggtttc
      961 acaaatatat ttgaacaaac tagccctgat tttgtattat tactagggga tagaggagaa
     1021 atgttggcag ctgcgatagc ttgtttgtat cttggaatca aaacagcaca tattcacgga
     1081 ggcgaagtgt ctggtactgt agatgaatca ataagacatg ccataacgaa aatagcatct
     1141 gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa taaaaatggg ggaagattct
     1201 tggagagttt ttcccgtcgg agctcctaga atagatacta ttttaaataa tagattacct
     1261 agttttagag atataaaaga aaaatatcat ctaaaatttg aaaagaaaaa atacgttttg
     1321 caagtatttc atccagttgt aaccgaacta gaaaatatag aaaaacaaat tttagctttg
     1381 atcaattcaa tggaacaaca atctcaacca attatttgca ttttacctaa tagtgatgct
     1441 ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa aaacggatat tatttatatt
     1501 acgaatttag agccggagga atacctgact atattaaaag agtgcaaatt tatgatcgga
     1561 aattcttctt ctgggataat tgaagctgca tcattcaaaa taccagtaat aaatttagga
     1621 accagacaaa atggacgaga acaatctaaa aatattataa atgcaaatct aaatgaaaat
     1681 gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt ttataaaaag cttgaaaaat
     1741 gttaagaaca tctacggtga cggcaacagt tctagaagaa taacaaaaac attgaatcaa
     1801 atattgaatg atggggaaga tattaagtgg attcaaaaac aaatagctta ttaaaatata
     1861 ctagtgatcc atacattatt attggggctg gcggacacgc aaaagtcatt attgaaacct
     1921 taagattaga aaataagtta atatatggat tttttgatga taagcaaaat aaaatgcaag
     1981 atctgaaata tcttgggaaa ataaacgaat tgaatagctg ggcggatagc aaaaaaaaaa
     2041 tctataaatt tattgtagct attggtgact gtgtagtaag aaaaaagatt ataaaaaaat
     2101 tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc aagtgctata gtggcaaaag
     2161 aaatttcaat tggttatgga actgttattt ttgcgaatgc tgtaattaac ccagatgctg
     2221 taattggaga gcatgctata ataaacacag gtagcattgt tgagcatgat tgtagaatta
     2281 ataactatgt tcatttatct ccaggtgtat gcttagcggg tggagtacat gtaggtgtag
     2341 gaactcaaat aggaattggt tctcaatgta tccagatgaa gaaaattgga tcttggtgta
     2401 tgataggcgc agggagcact attgttaagg atattccctc acattcctta gcttatggca
     2461 atccagctaa aattaagaaa gaaggaatta attttgaatt ctagaatttt attatcaagt
     2521 cctcatatgg gaggaacaga agaaaaatat gttgctaagg cttttaaaga taattggata
     2581 gcgccattag gtgaaaatgt ggataaattt gaagaaagta ttacaaaata tactggttct
     2641 aagggcggag cttctgcgtt aagttcaggt acagctgcca tacatcttgc cttaagactg
     2701 cttaatgttg gaaaaaacga tattgtcttt gctccttcat ttacttttat agctacagta
     2761 aatcctattt tatatcaagg ggctgtacca gtgttcattg actcagaacc taatacatgg
     2821 aatatgagcc ctgttgcact agagagcgca ttagaagaag ctgaaagaaa caaccaatta
     2881 cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg ctgattatga acgaatttta
     2941 gctattgcaa accaatttaa tgttccagtg atagaagatg cagcggaatc gttaggttct
     3001 acttataagg gaatttcgtc aggcttattt gggacaattg ggatctattc atttaacggt
     3061 aataagatca tcacaacctc aggtggagga gctctagtgt ctagtgataa aaaatatgtt
     3121 gagcattcaa gatttttagc aacacaagca agagatcaag caccgtatta tcaacatagc
     3181 gaaataggtt ataattaccg tctaagcaat atttctgccg gtatcggacg tggacaaatg
     3241 gaagtattga atgatagagt agctaaacga agagaaattt tcaatcgata taaggaagct
     3301 ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg atggcatggt gccaaatcgt
     3361 tggttgtcta ctttaacgat caatgattat aattctattg atcaagtagt taacgtaata
     3421 aataaactaa atgaaaaaaa cattgaaaca agagctttgt ggaaaccact acatttgcaa
     3481 ccagtatttc aaggggtaaa aatgtattac catgataata atcttagtgt gtcagaaaag
     3541 ttatttaata gcggcttatg cttaccttct ggaagtaata tgacagatag tgaacaagaa
     3601 tttgttattg atgagtttaa aaaaatattc aagtaggtgt gatattttat gaacatagga
     3661 attattggtt taggttccat ggggaaaaga agacttagac tcattcaaga acactttaat
     3721 gatattttac tatgtggtgt tgatactaat gaggcgagac gaaaggaagt tgaaaaggaa
     3781 tttggaatac caacttataa tagtctaaca gaggcaaaaa atttgtttga aataaattca
     3841 gtttttgttt gtacatcccc tataacccat gaatttatta taaaagaggc tttaaattta
     3901 gattgtaata tctttacgga aatcaattta ttaaatgaat attatgatga aataatcgaa
     3961 gtagctaaaa agaaagatag attactttat ctatcttcta catttttgaa gagaaaagaa
     4021 attcaatata ttaagtctaa agtgacgcat aataaaaaaa tttcttatca ataccatgta
     4081 ggacagtatt tacccgactg gcatccttgg gaagattatc gcaatttttt tgtttcaaac
     4141 aaaaaaacta atgggtgtag agaaatattc gctattgaat taccttggat tatagataca
     4201 tttgggaaag ttaaatcgtt tactgtaatg acccagaaat tatccaatct tgatattgat
     4261 tattcggaca gtttcactct tattattgag catgaaactg gtatagttgg aactttaagt
     4321 gtagatgttg tttcaagagt agctaaaaga aatttaacta taattggtga aaagatttta
     4381 atagaatggg aaggtaaccc ggattctcta ttttcatgga atgaagaaac ttctagtatg
     4441 acagatattc aattgtacga tgaggttatg catgataata attattcaca tactattatt
     4501 gaagatgcat atttagagga gattaaagaa tttttcacgc ttttggaaaa gaaaatggaa
     4561 ccccaatata cttttgaaaa agataagtat attattaact taattaacga cattgaggta
     4621 aattaatgat gaaagtaatt ttttttgggt tagggtcaat tggaaagaga catctaaaaa
     4681 atctaataag ctatggaaaa aagaattcta ttaattttga tataacagct tatagtagca
     4741 gtggaaaaaa agagtttgaa gaaataacgt atattcaaaa tagagacgat ttggatacac
     4801 attatgatat agcttttatt acaaatccga cttttaagca tctggaaaca ctaaatgtaa
     4861 ttgttgaaaa agttgattac atatttttag aaaaacctgc tttttctgag agtattgatc
     4921 ttactcaata taattatgat tacaaaaata tttatgttgc tgctccactg aggtataaaa
     4981 atatcatgga agttgttcgt aatagtattc aaagtaaaaa agtatattct gtaagagcta
     5041 tttgttctac ttatcttcct gattggagaa aaggtactga ttatagaaat aattattctg
     5101 cctttaaatc tatgggggga ggagtagaat tagactgtat acatgaattg gattatatta
     5161 taaacctatt tggtttccct aaatcagtaa aaaaacagtt tggtaaatat tcgaatcttg
     5221 aaattaacag taatgattta gcaacatata tattgagata cgatgataaa attatagaaa
     5281 ttcatttaga ttattttgga agagttccta caagaaaatt tcaattgttt actttagatg
     5341 aatgtatcaa tgttgactta ttaaataata aaataagttt tgaagaaaac attgttaaag
     5401 aaatgcctga atctgctaat gatatgtatg agaaggagtt gaaatatttc tttgaaaacg
     5461 tagtaacagg caaagaaaac tggaatgatt tatatcatgc aaacgaagta ttgaaaatag
     5521 ctgaggagaa ataaatggaa attttattta ctatttgtgg tagagctggg tctaaggggt
     5581 ttaaaaataa aaatttgaaa tatttggcag gaaagcccct agtccatttc gctttagcaa
     5641 tgatagatat ctatcaacaa aaacatcctg aacataattg cacgacttgt ttgaatacgg
     5701 acagtaatga gttagtagaa ttgacgacta agttaaatcc gtctgtgaaa attgtagaac
     5761 gtaaacctga attagctaca gatactgtag ggaaaattga agtgatacgt gattcattct
     5821 tacaactaga aagagaagat aatatatttg atttaattat agatttagat ttgacttcac
     5881 ctttacgtga agtttctaat cttgaagaat taatagatga acatctaaaa aatttagata
     5941 aagacgtcta cttttcagtt gtttctgcaa
//
LOCUS       DAZKMK010000125         5924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000125 DAZKMK010000000
VERSION     DAZKMK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.128"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..608)
                     /locus_tag="ACG8V1_002635"
     CDS             complement(150..608)
                     /locus_tag="ACG8V1_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0330666.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(621..1952)
                     /locus_tag="ACG8V1_002636"
     CDS             complement(621..1952)
                     /locus_tag="ACG8V1_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0330667.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1963..3405)
                     /locus_tag="ACG8V1_002637"
     CDS             complement(1963..3405)
                     /locus_tag="ACG8V1_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0330668.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3392..4156)
                     /locus_tag="ACG8V1_002638"
     CDS             complement(3392..4156)
                     /locus_tag="ACG8V1_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0330669.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4260..5135)
                     /locus_tag="ACG8V1_002639"
     CDS             complement(4260..5135)
                     /locus_tag="ACG8V1_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0330670.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5390..5474)
                     /locus_tag="ACG8V1_002640"
     tRNA            complement(5390..5474)
                     /locus_tag="ACG8V1_002640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5438..5440),aa:Leu,seq:caa)
     gene            complement(5492..5563)
                     /locus_tag="ACG8V1_002641"
     tRNA            complement(5492..5563)
                     /locus_tag="ACG8V1_002641"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5529..5531),aa:Gln,seq:ttg)
     gene            complement(5573..5645)
                     /locus_tag="ACG8V1_002642"
     tRNA            complement(5573..5645)
                     /locus_tag="ACG8V1_002642"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5610..5612),aa:His,seq:gtg)
     gene            complement(5707..5791)
                     /locus_tag="ACG8V1_002643"
     tRNA            complement(5707..5791)
                     /locus_tag="ACG8V1_002643"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5755..5757),aa:Leu,seq:caa)
     gene            complement(5812..5882)
                     /locus_tag="ACG8V1_002644"
     tRNA            complement(5812..5882)
                     /locus_tag="ACG8V1_002644"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5848..5850),aa:Cys,seq:gca)
BASE COUNT         1818 a         1258 c          899 g         1949 t
ORIGIN      
        1 taagttttct aaatcatagg agcaacaaaa aaattcgata attaacaaaa atcaaaatga
       61 ttaaatctag tgattgaaac atatcatttc aaaaaaattt tgtaaattca cccataaata
      121 tcaccctatt taactttttt catcgtacac taaaattcaa aacaactatc tttaatcttt
      181 acttcttggt tttgttttaa taaatttcct tcaaaaatgc ctggagaaac tacaatgatc
      241 accgtatctt tttgtgcttg ctccaacttc ttcaaatcta taatagctaa cagtgtccct
      301 tcttttacct cctggttact ttcaacccgt atctgaaacg gacttccttc gagttcaact
      361 gtatctaaac ccatatggat cagtattttt ctgccagatt cactttcgat cgttatggca
      421 tgtcgggtag gaaatatatt atcgattatt cctttaactg gcgcatggat atatccatca
      481 tgattcgtta tagcgtatcc aactcctatc atttttgaag aaaagacctc gtcttttact
      541 gctgttaatg gaataatttg accactagct acagctttta gaatgtttct tgttttaaag
      601 aaatgcactt tttatcacct ctactttaat gctggccaat attttttgtt tactttgatc
      661 atttcatcta gtattttttt tgcgactctg gcatttggta cggtttgatt caatgtaaat
      721 gcttgtaaag ctttttgata agaaccttca aagtaagcat ctactagtaa cttctctact
      781 gcaacttgtg cctccatcaa tcctttatga aaatcactga ttggttttct tagtgtaatt
      841 ggctccacac cagttgcacc aacatatgcc ggtatttcaa caactgcatc tgaccgtaga
      901 ttcggaatcg ccccttgatt aggaacgatt agcataaagc gacgacgatc atcattcaac
      961 aaggaaatag ctatatcgac gatatattgt ccatgctccc caaaattaaa gttcaatact
     1021 tcttccacaa taccgcggcg tattttatca gccatttctt tggtattttt ctctcgtcca
     1081 tccatgacca tattagcacg tgtatactct ttgtcagtat gctcaacgac catatcagga
     1141 tataagtaat actctaagta attattcggg atattacttg gaaaattttt cgtgatgact
     1201 gacatcattt gaaaggtttt ttgccaagtt ttatctccta tattaaaatc ggcgacttgc
     1261 atttcttttg ttattagttt ttcaataatc tctggcataa tttctcgttg caattcttta
     1321 tcataaatcg atgtatacca gccaaagtgg tttaaaccat aatacgtaac gatccaattc
     1381 ttacgatcat aaccgtaatt cactgcaatc gtttcttcaa ttgaaatagt catatcacag
     1441 gcattaatca tttttacatt cggatattta cgacgaacag cttcagaaac gatcgactct
     1501 ggatttgtat aattcaaaat ccatgctttg ggggcatagt cttgtatatg atcgacaatt
     1561 tccaataaac ctttgattga gcgcatccca taagcaaatc ctccgagtcc acacgtttct
     1621 tgtcctacta atccatgttt aagtggaatc ttttcgtcct tttctcgcat ctccaatcct
     1681 ccaacacgaa tttgtgagaa aacaaaatcg catcctgtaa aagcaagctt tggatcatcc
     1741 gttgcacgaa tgcgaatatt tgaaaaacct tttcttttca gcataaattt aacaatcaag
     1801 tacatatcca tatttctttc ttcatcaata tcatataaac gtatttcaga taatggcaac
     1861 cgtttttcat tgttcaaaat ggcttgaacg attcctggtg tatacgtact cccgcctcca
     1921 gctatagtga taatctgttt tttcatcatc atcttttcct ttctattctt ctaataattt
     1981 attgaattct gccataacat aagtcacttc taagccgatg ataatttgat aatttttttc
     2041 agttggttta atgatcccgc ttatccctaa ttgcttgatt cgctgttcct cgaccatggt
     2101 gggatccact acctctaccc gtaggcgtgt attacaacta tagacatcca ggatattggc
     2161 ttttcctcca aacgcttgta gcatttttgt tgccatatat tcataatttt tacttgctaa
     2221 ccctaactct gtttcttcta aggttacttc attgccaaac tctgccttag cttcacgtcc
     2281 taatgtctga atgtttctct tattgatcaa ataataaaaa gtaacgtaat acaaagcaaa
     2341 aaagaaaatt ccgattggaa gaatcaaaat agcattggta gcgattcgaa aattgatgag
     2401 ataatcaaca atacatgctc cgaatgtaaa accaattcgt atattgaata agtaacagac
     2461 aatccctgct aaaccggcaa aaagtgcatg gatcacgtac aacataggag aaagaaacat
     2521 gaaactaaat tcaacaggct cagtgatatt tgcaataaat gaagttcctg caccgcttcc
     2581 catgattcct ttgaccatct ctttattttc tttataagct gctttataga tagctagaca
     2641 tatagctggt ataccaaaca tcattactac aaaaaaaact gataaaaaca gtccagcctg
     2701 aggatctccg ttgataaagc gtgtcatctc tcctctgaaa acttccttgc taggagatgt
     2761 atagcttcct aaatcgtaat aaatatacgt attcaacaca tggtgcagtc caaaggggat
     2821 cagcaaacga ttcaaaaaca taaatatccc cacacctaat gctcccatat caacgagcag
     2881 ttttgcaaaa ttatcaatcc caatttggat gagcggccag ataaagccca aaaaaacagc
     2941 tgcgattgtt tgtagaatca tcaccatagt caatgggaat ttttcgcccg caaaataaga
     3001 aaaaactact ggtaattttt gttctttaaa gcgattatac gtccatgccg ctactaaacc
     3061 ggaaataatt ccaccaaaaa tccccatatt cacttctggg tccataattt ctaagccttt
     3121 tttcaaggta gcaatcgcta aaaaaccagt aagtgcaggt attcctttgt ccttattttt
     3181 gacaaaaccg attgcaactc ctatagcaaa taagatatcc aagtttccaa aaacggcatt
     3241 tccagctgca ccaattactg ggatgttcag catatcgtca gctgataaac gcatgatcaa
     3301 tcccgctaat ggcatcgctg caactggaag tagcatcgct cttccaagct tctgcacatt
     3361 tgatttaaca ttcattttta ttcctcctct attaaatatc ctcatatgtt actaactcaa
     3421 tcaaaggatc atttttcact ttttgagcaa atgctgaatg aattaaaaat tcgacttggt
     3481 gggtacgagg atacacaaaa cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt
     3541 gacacatgac ttcgatcgtt ttatcttctc tcaatatcat gagtaaactt gccaaatatt
     3601 cttccacact cgtattgatt tgtcgttttt tttcatcagt ttctcctacg acatcgaaac
     3661 aaggctcaaa ataatcgaca tgacgaactt catcttttcg ttcaaagttg ctccgaaccg
     3721 gcaaatcata tttttttgct aatgtaacaa ctacctcctg attttcatcc aaagtatgag
     3781 tatgatgatg actgtctaaa tgagttggta tgatccccgc atgataaatt ttttgaatct
     3841 gagcattcca ttcctgatac agttgatctt tgtcaatgtt aaatggatgc tgataataag
     3901 ataaggatct gaactctccg ctttcgataa gagtatcgac agttttgagg agtggtttgc
     3961 cacacgtcaa tgttaaatgg actcctacac ctagcctagg catttccttt cttagtttca
     4021 cagcatgctc gaaacccggc atatttgcca tcaatgtagt tgatgtcaaa attcctcgct
     4081 gataagcatc cataatccca taattcacac catggctata gccaaaatca tccgaattaa
     4141 aaatcacttt tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata
     4201 atcatagtcc atttactatg tgtcatttgg ttgcgcctat agatagttac gcccttattt
     4261 taatagattc ttgctagatg aattgccaaa tagatgattt cattttcttg aatggcaagt
     4321 tgaaattgct gttcgatgat atctttaatc tttagtgctg tacaatacgc tttgctatgt
     4381 ttaaatgttt cagtaaaaaa accaatattt tctactggcg tttcttcctg ataaatcaag
     4441 cgtttaacca ggaatttgat atgaaccatt aagcgatgat aagtcagtga ttctttatca
     4501 taaacgtttg aataggattt ttctagatgg gacaaaatcg tggtcgtcat ttctactatt
     4561 tttaagctct ttaaactatt aggctctcct agctcagcat taatgatatg catagtgatg
     4621 aatcctactt catttgaccc taactcggtt tgatatcgat gattgattcg ctcgaccact
     4681 tctactgcta tatcatattc atttggataa aggaatctca tttctggcaa cataattgct
     4741 gtgacatctc tatcttctct ttttcgttga atagcaaaat aaatatgatc tgtcaatgta
     4801 atatagatat ttgaatctaa tggagaggag atcctagctg ttatcagttc aatcacttct
     4861 tccgcaaaat ccataaattc gattggaatc tgcgagacta tctcactgaa tcgtttgatc
     4921 ttttctttag aatctaatac aaataatttg tcagcatcct caacatcaaa tggcttactc
     4981 actttagcag aaaagccaat ccctttcccc atcgcaattt tttctattcc atcgtcatcg
     5041 atcaatacag cattatgatt cagctttttc atcacataca ccgttaattc acctcctcta
     5101 tatgataaaa caaaacaact tgcagactcc ttcatacata tttttgtctc caaacaattt
     5161 ttaaagacaa aaaacgacct aagaaagaac cctttcttag gtcgtgccta attgaatagt
     5221 agcaccctac ataatttaca aaaatagttt aacacataat aaaagcgtta tcaatatctt
     5281 ttttactcgt tttatgataa tacttgtatc gaattgattt ggaggtaatt acataaaaac
     5341 aaaaaaaata tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc
     5401 ggggtcgaac cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat
     5461 tccgccacgc cggcaaattt aaaaataata actggggtag ctggattcga accaacgcat
     5521 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa aaggcgaatg
     5581 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
     5641 ttcgccatca taaaagatgt tatttaattt ctcaataaaa aactaagatg aagacatctt
     5701 agtcaatacc ggcggccggg gtcgaaccgg cacgccctca acgggcactg gattttgagt
     5761 ccagcgcgtc tgccaattcc gccacgccgg caagttgatt tattagtact aaggcggtaa
     5821 ccggatttga accggtgatg aaggttttgc agacctctgc cttaccactt ggctataccg
     5881 ccattatact attatcaatc tataaaataa agaataaatt atag
//
LOCUS       DAZKMK010000126         5879 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000126 DAZKMK010000000
VERSION     DAZKMK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.129"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..684)
                     /locus_tag="ACG8V1_002645"
     CDS             complement(37..684)
                     /locus_tag="ACG8V1_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0330671.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(692..1378)
                     /locus_tag="ACG8V1_002646"
     CDS             complement(692..1378)
                     /locus_tag="ACG8V1_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330672.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1384..2457)
                     /locus_tag="ACG8V1_002647"
     CDS             complement(1384..2457)
                     /locus_tag="ACG8V1_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0330673.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2679..3353
                     /locus_tag="ACG8V1_002648"
     CDS             2679..3353
                     /locus_tag="ACG8V1_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0330674.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3358..4698
                     /locus_tag="ACG8V1_002649"
     CDS             3358..4698
                     /locus_tag="ACG8V1_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0330675.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4847..5833)
                     /locus_tag="ACG8V1_002650"
     CDS             complement(4847..5833)
                     /locus_tag="ACG8V1_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0330676.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1774 a         1124 c         1039 g         1942 t
ORIGIN      
        1 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
       61 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
      121 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
      181 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
      241 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
      301 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
      361 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
      421 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
      481 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
      541 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
      601 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
      661 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
      721 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
      781 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
      841 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
      901 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
      961 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
     1021 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
     1081 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
     1141 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
     1201 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
     1261 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
     1321 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
     1381 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
     1441 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
     1501 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
     1561 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
     1621 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
     1681 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
     1741 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
     1801 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
     1861 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
     1921 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
     1981 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
     2041 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
     2101 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
     2161 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
     2221 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
     2281 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
     2341 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
     2401 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
     2461 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
     2521 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
     2581 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
     2641 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
     2701 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
     2761 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
     2821 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
     2881 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
     2941 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
     3001 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
     3061 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
     3121 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
     3181 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
     3241 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
     3301 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
     3361 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
     3421 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
     3481 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
     3541 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
     3601 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
     3661 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
     3721 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
     3781 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
     3841 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
     3901 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
     3961 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
     4021 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
     4081 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
     4141 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
     4201 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
     4261 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
     4321 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
     4381 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
     4441 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
     4501 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
     4561 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
     4621 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
     4681 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
     4741 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
     4801 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
     4861 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
     4921 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
     4981 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
     5041 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
     5101 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
     5161 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
     5221 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
     5281 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
     5341 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
     5401 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
     5461 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
     5521 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
     5581 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
     5641 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
     5701 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
     5761 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
     5821 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttact
//
LOCUS       DAZKMK010000127         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000127 DAZKMK010000000
VERSION     DAZKMK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.130"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8V1_002651"
     CDS             complement(488..1297)
                     /locus_tag="ACG8V1_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0330677.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8V1_002652"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8V1_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0330678.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8V1_002653"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8V1_002653"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0330679.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8V1_002654"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8V1_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0330680.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8V1_002655"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8V1_002655"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0330681.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8V1_002656"
     CDS             4179..4913
                     /locus_tag="ACG8V1_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0330682.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG8V1_002657"
     CDS             4897..5607
                     /locus_tag="ACG8V1_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0330683.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKMK010000128         5504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000128 DAZKMK010000000
VERSION     DAZKMK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.131"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      27..124
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            240..821
                     /locus_tag="ACG8V1_002658"
     CDS             240..821
                     /locus_tag="ACG8V1_002658"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0330684.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            824..2131
                     /locus_tag="ACG8V1_002659"
     CDS             824..2131
                     /locus_tag="ACG8V1_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0330685.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2547..3035
                     /gene="purE"
                     /locus_tag="ACG8V1_002660"
     CDS             2547..3035
                     /gene="purE"
                     /locus_tag="ACG8V1_002660"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0330686.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            3028..4152
                     /gene="purK"
                     /locus_tag="ACG8V1_002661"
     CDS             3028..4152
                     /gene="purK"
                     /locus_tag="ACG8V1_002661"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0330687.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4165..5460
                     /gene="purB"
                     /locus_tag="ACG8V1_002662"
     CDS             4165..5460
                     /gene="purB"
                     /locus_tag="ACG8V1_002662"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0330688.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
BASE COUNT         1709 a          942 c         1287 g         1566 t
ORIGIN      
        1 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
       61 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
      121 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
      181 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
      241 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
      301 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
      361 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
      421 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
      481 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
      541 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
      601 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
      661 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
      721 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
      781 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
      841 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
      901 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
      961 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
     1021 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
     1081 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
     1141 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
     1201 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
     1261 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
     1321 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
     1381 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
     1441 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
     1501 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
     1561 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
     1621 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
     1681 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
     1741 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
     1801 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
     1861 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
     1921 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
     1981 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
     2041 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
     2101 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
     2161 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
     2221 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
     2281 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
     2341 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
     2401 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
     2461 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
     2521 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
     2581 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
     2641 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
     2701 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
     2761 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
     2821 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
     2881 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
     2941 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
     3001 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
     3061 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
     3121 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
     3181 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
     3241 gtcattacct atgaattcga aaacgtcagt gtagaggcgt taaatgcgat tttacccatg
     3301 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
     3361 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
     3421 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
     3481 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
     3541 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
     3601 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
     3661 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
     3721 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
     3781 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
     3841 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
     3901 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
     3961 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
     4021 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
     4081 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
     4141 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
     4201 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
     4261 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
     4321 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
     4381 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
     4441 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
     4501 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
     4561 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
     4621 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
     4681 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
     4741 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ctctactcaa
     4801 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
     4861 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
     4921 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
     4981 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
     5041 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
     5101 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
     5161 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
     5221 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
     5281 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
     5341 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
     5401 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
     5461 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaa
//
LOCUS       DAZKMK010000129         5479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000129 DAZKMK010000000
VERSION     DAZKMK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.132"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..407)
                     /gene="ssrA"
                     /locus_tag="ACG8V1_002663"
     tmRNA           complement(41..407)
                     /gene="ssrA"
                     /locus_tag="ACG8V1_002663"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(578..1351)
                     /locus_tag="ACG8V1_002664"
     CDS             complement(578..1351)
                     /locus_tag="ACG8V1_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0330689.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1495..1845
                     /locus_tag="ACG8V1_002665"
     CDS             1495..1845
                     /locus_tag="ACG8V1_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0330690.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1826..2542
                     /locus_tag="ACG8V1_002666"
     CDS             1826..2542
                     /locus_tag="ACG8V1_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0330691.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2542..3990
                     /locus_tag="ACG8V1_002667"
     CDS             2542..3990
                     /locus_tag="ACG8V1_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0330692.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4060..4569)
                     /locus_tag="ACG8V1_002668"
     CDS             complement(4060..4569)
                     /locus_tag="ACG8V1_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330693.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4559..5284)
                     /locus_tag="ACG8V1_002669"
     CDS             complement(4559..5284)
                     /locus_tag="ACG8V1_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330694.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1862 a          955 c         1095 g         1567 t
ORIGIN      
        1 ataaggattt aagcatctag cgaaatctgc tgaaatctca caatggagac ggcgggagtc
       61 gaacccgcgt ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag
      121 gattcacttt acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt
      181 tttcaactta cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag
      241 cacatgggcg atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag
      301 ttgttttcgt ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg
      361 aaacgcaatt caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag
      421 ggttacgtgt atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa
      481 aagcgaatca tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg
      541 attttcttaa gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca
      601 atgcgtggac aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta
      661 atccgtcata ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat
      721 cattcggacc atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca
      781 gacgtttgac acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac
      841 cattccgata aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag
      901 ataagatcag caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa
      961 catctgaatg aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga
     1021 ttttctgatc gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt
     1081 tttgacattc ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga
     1141 aagaaccatt catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt
     1201 ctgcttctgt acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca
     1261 tagcttttgc cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat
     1321 caaagaaagt gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag
     1381 tttatcttaa caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt
     1441 ggttcagtca acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt
     1501 tttttcagta ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca
     1561 aaaggaaatc tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat
     1621 tggcggctgg ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca
     1681 ttgatcggct attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt
     1741 ctgctagtat ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat
     1801 gacctggaaa ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt
     1861 ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga
     1921 tggaaaagaa gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact
     1981 agacctgaat ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc
     2041 aagtactgtg ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg
     2101 cttagagatg ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc
     2161 tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagtagt
     2221 ttcagatgat cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga
     2281 agcgtatctg gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt
     2341 tactttagca aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg
     2401 ggattatcag tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca
     2461 aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt
     2521 cgatgattct ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttctgcgga
     2581 atggtcgcgt tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg
     2641 attgaagaaa acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg
     2701 caaacctatg ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg
     2761 ctgctgacag agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac
     2821 gaacgtgtca tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa
     2881 tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga
     2941 caagcacagg cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga
     3001 gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg
     3061 aacctgttca aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt
     3121 gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat
     3181 ggtaattttg atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat
     3241 gatttcaata aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa
     3301 aatcgtcgaa gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca
     3361 atcaacggac ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac
     3421 gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg
     3481 gattatgaaa aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca
     3541 gaaacattga aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca
     3601 ttgacattga aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa
     3661 atcatggtca atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt
     3721 actttagaaa aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg
     3781 tctgaacaac aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa
     3841 aatacaaaat atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg
     3901 cataaaggaa aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt
     3961 ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg
     4021 atcttctata ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg
     4081 atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt
     4141 ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag
     4201 ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa
     4261 ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg
     4321 agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac
     4381 atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat
     4441 gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt
     4501 atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc
     4561 aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga
     4621 atagactaag aatgagacag catccactga gcccaataag agtttgggca aatcccttaa
     4681 gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca
     4741 tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag
     4801 tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta
     4861 cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa
     4921 taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc aattcctgct
     4981 tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata
     5041 aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg
     5101 tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt
     5161 gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac
     5221 aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt
     5281 tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc
     5341 tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag
     5401 aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag
     5461 gagcagcgac aataatttt
//
LOCUS       DAZKMK010000130         5393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000130 DAZKMK010000000
VERSION     DAZKMK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.133"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(118..393)
                     /gene="prgO"
                     /locus_tag="ACG8V1_002670"
     CDS             complement(118..393)
                     /gene="prgO"
                     /locus_tag="ACG8V1_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0330695.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(365..1318)
                     /locus_tag="ACG8V1_002671"
     CDS             complement(365..1318)
                     /locus_tag="ACG8V1_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0330696.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1930..3423
                     /locus_tag="ACG8V1_002672"
     CDS             1930..3423
                     /locus_tag="ACG8V1_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0330697.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3558..3854
                     /locus_tag="ACG8V1_002673"
     CDS             3558..3854
                     /locus_tag="ACG8V1_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0330698.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3955..4806
                     /locus_tag="ACG8V1_002674"
     CDS             3955..4806
                     /locus_tag="ACG8V1_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330699.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4827..5039
                     /locus_tag="ACG8V1_002675"
     CDS             4827..5039
                     /locus_tag="ACG8V1_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330700.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1848 a          835 c         1010 g         1700 t
ORIGIN      
        1 tgtgaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
       61 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      121 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      181 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
      241 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
      301 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
      361 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
      421 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
      481 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
      541 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
      601 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
      661 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
      721 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
      781 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
      841 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
      901 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
      961 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1021 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1081 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1141 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1201 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     1261 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     1321 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     1381 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     1441 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     1501 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     1561 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     1621 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     1681 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     1741 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     1801 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     1861 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     1921 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     1981 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2041 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2101 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2161 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     2221 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     2281 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     2341 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     2401 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     2461 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     2521 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     2581 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     2641 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     2701 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     2761 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     2821 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     2881 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     2941 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3001 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3061 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3121 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3181 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     3241 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     3301 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     3361 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     3421 tgaaaaataa aaggtgcaaa ttgccctata caatatatct atgatacttc tgtaggtgtt
     3481 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     3541 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     3601 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     3661 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     3721 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     3781 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga aaaaggaaga
     3841 agtatatcca gtagtagttt ttatttactt aaaaaattgg ttcattgagc aacctatcgc
     3901 tgctaaagta gcaaaggaaa acgttaaagg aaagaattta gggaagaaat cgaaatgaaa
     3961 tcggacgact ggattgaaca cacctggctt cctctgaaaa gaaatagcaa acgaaagaaa
     4021 ttttgggtat tgcaattatg gccattagga aaactttgta gttacggggc acgacttatt
     4081 cttgatttaa ccgtttactc gattggagta tttggaacgg cttattactt gataaatgat
     4141 cccaatagga ttgcagcatc agacattttt acagtgctaa tgacgttgat ttttgtcatg
     4201 ctattgtcta tttttactca attagtcatg caatttacca ggcaaaaatt aacagcagga
     4261 aaacttttca aaagcctatt cgatttaatt gttgtaaatg caattctagc gactttaata
     4321 aaacaaattg aacaatctgg aagaacaaca aaaggagtga aggatttttt attgacgttt
     4381 attcactttg taaatggaca catgtatttg tttgaagtgg gtattattct cttttttgtc
     4441 gggtatgtat ccacgaaact tattaaacgt tcaattgaaa aagaaattga aattgacctg
     4501 attccgacac ttcttggtta tgagtacaat gttgattttg gaagatatga cgaattgatc
     4561 aatacggata tcgttcacac gagtatccat gaatctactg ttaagggcat cagatctatt
     4621 ttaaaaaatg gccaggaaat gaaagcaata gatcgaagaa aagagatcta caatttttat
     4681 gaagtcgaag caagcctacg atatggcatc ggaaaattgt ggttagatgg aagtaatatt
     4741 cgtcaaaaca aaccatacat tttttggaac agtagtgatt tagaacttcc aatgaaagat
     4801 aaataattat ggagaatcgt ttgaaaatga ttcctctata caggattgtg cttatagttg
     4861 gcatggagtt aaaaacctta aagaaggagc agttattcaa agtgaaagaa cgtcataatc
     4921 agttactatg tagtaaaacg ggtacgatga acattgaata ccgaacagaa tatttagaat
     4981 taacgaatca aactgatttt ttgttgattc gaaagcaaca agagcaagaa aaaaattaat
     5041 ggctacttat agccaattga ttgatgaatg ggaacaattc aaggaatcta aaattccaga
     5101 acgtctctaa aagcctttat ttgtaaagag cataaggaaa cgtgggaaca ctttttaaaa
     5161 aaagtgttcc aataggctgt gcctattacg tttctcaaaa ataaatgtgg gaacaaaata
     5221 tttaaaaata ggatagtgaa aggatagtga aggatagtaa aaggagggta gtaggatagc
     5281 aaaaaggata ggctcctttt cgctatcctt tttaaattgg aggaattaat atggaagtaa
     5341 gaataagaaa tgtggatgag aaaatcgtaa gagaaattga tcgaagatgt gac
//
LOCUS       DAZKMK010000131         5312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000131 DAZKMK010000000
VERSION     DAZKMK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.134"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8V1_002676"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8V1_002676"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0330701.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8V1_002677"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8V1_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330702.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8V1_002678"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8V1_002678"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0330703.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8V1_002679"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8V1_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330704.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8V1_002680"
     tRNA            3917..4000
                     /locus_tag="ACG8V1_002680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5312)
                     /locus_tag="ACG8V1_002681"
                     /pseudo
     CDS             complement(4240..5312)
                     /locus_tag="ACG8V1_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAZKMK010000132         5191 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000132 DAZKMK010000000
VERSION     DAZKMK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.135"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG8V1_002682"
     CDS             137..748
                     /locus_tag="ACG8V1_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0330705.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG8V1_002683"
     CDS             821..1750
                     /locus_tag="ACG8V1_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0330706.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG8V1_002684"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG8V1_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG8V1_002685"
     CDS             complement(2693..3895)
                     /locus_tag="ACG8V1_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0330707.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG8V1_002686"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG8V1_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5191)
                     /locus_tag="ACG8V1_002687"
     CDS             complement(4706..>5191)
                     /locus_tag="ACG8V1_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0330708.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1641 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta a
//
LOCUS       DAZKMK010000133         5137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000133 DAZKMK010000000
VERSION     DAZKMK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.136"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..447)
                     /locus_tag="ACG8V1_002688"
     CDS             complement(<1..447)
                     /locus_tag="ACG8V1_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330709.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(754..1851)
                     /locus_tag="ACG8V1_002689"
     CDS             complement(754..1851)
                     /locus_tag="ACG8V1_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0330710.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1959..3986)
                     /locus_tag="ACG8V1_002690"
     CDS             complement(1959..3986)
                     /locus_tag="ACG8V1_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0330711.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4131..4859)
                     /locus_tag="ACG8V1_002691"
     CDS             complement(4131..4859)
                     /locus_tag="ACG8V1_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0330712.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1040 c          813 g         1828 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat attgttataa aaagagccca accatcctac
      721 aataataaaa atttactatt tgttatctta accttacttt tctttctctt ctttttttct
      781 caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag ctactaagat
      841 ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt ccttggctac
      901 ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt tgatcgtagt
      961 atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa cagtcattgt
     1021 aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa aagaaaaatt
     1081 tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt cagaatgtcc
     1141 tttttttgta atttttgttt caataactac ttgattgata taggtttttt gatcattttg
     1201 aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt catgaagagt
     1261 aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa tataggtgta
     1321 ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt ccgtctggat
     1381 ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg gcatcctgac
     1441 atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg gatagctcac
     1501 ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt ttccgtattc
     1561 aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt tcacttcttt
     1621 ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat caagttgaag
     1681 atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg tcactgaaaa
     1741 attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa taactaggag
     1801 tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca tatgtaagag
     1861 aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc tgtgcccttc
     1921 ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg gacatctgca
     1981 agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac aggaatattt
     2041 attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct ttcacccttt
     2101 tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt ttcagaggta
     2161 acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt ctcttgtttc
     2221 aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc acctgaaaac
     2281 tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt gacatattga
     2341 ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc ctctgtattt
     2401 tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc ttcaatatca
     2461 ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat ttcagcaata
     2521 tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt cagctttgcg
     2581 atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag gtattcaaaa
     2641 tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa ttcgattatt
     2701 aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt tccggttatc
     2761 aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg aatagttaat
     2821 tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc cccctcgacc
     2881 ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat ggccccataa
     2941 tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca
     3001 gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca atttacttct
     3061 gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagcgacc gacaccttcc
     3121 aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg
     3181 ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga ataattatta
     3241 ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc agcagttatc
     3301 tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc aatcgagagc
     3361 gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc catacctggc
     3421 gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa tgtaaaggtc
     3481 atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt aaatgtaaaa
     3541 tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga cctttctgct
     3601 gggttcaact gcagtaactt cccattttcc aatatggacg tgctagtacc gataggtgtt
     3661 tgaaaaattt tatcgataga aataatattg ggatcgtcag gcagctcaac cgcagaaatt
     3721 ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt ctacgttgtc agtggtctct
     3781 ccactactcg aaatacttaa atcctcactg ctttcttgtg tctggttgga ttctattaaa
     3841 ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc tatttccatg ctccaactga
     3901 ttcaattcca aagagtaccc tatgatcgga aagatattag tcactaagag actgcttaaa
     3961 atgagcacga tcctcctctt tttcaatata tcttttcctc ctgatcgtta aattcctaga
     4021 tgaactattc gtatccaaac aaatttagcc tagcctttga caatttttgc tcgattacct
     4081 cgcaagacaa tatgactacg tatttatttt taacagttct tttcagtaaa ctagttgtta
     4141 ggtgtatctt ctaatgtcca aactaattta gtggaatatt gttgcgctaa cttgacagat
     4201 ttaccgggta ccgaaagctg taccgcatct tgattttcat tttcatttgc accaaatcga
     4261 tagacccaag tccccacacc tgttcctgat ttagcattaa ttgctaacgt attgtttcct
     4321 cccggtgtca acgttaattc agaattcacc gtttgcggcg tatatgttgg atcaaccaaa
     4381 gaaacagcct gccctttcgt aaatttcagc tgtgctccaa ctagttcatc cccactggca
     4441 gttttaaact gttctggttg agagacactt aatttccacc cagataaggt acttctccta
     4501 tctgtcactt ggacatatgt cggttttcta gacccatcgt tcatcacatc agcagctgca
     4561 taatacgttt tatccgcagt agaaattttt tgagtcccaa atttgaattt tgatccataa
     4621 tcaatcgata atgcccctcc tgttccaggg cttggaggat ctgatggatc gacaggttta
     4681 ttgggatcag gattctctgg atcaacagga ggagttacac cctcatctcc tgcttcaaat
     4741 gtaatcgttc cttctgtgtt atattccttt gggtatactt cagttgcaga aacaaaagtc
     4801 gtacttgcta ccatgctgaa taacatcatt gctgctgtta atacgatttc ttttttcatc
     4861 attgacattc ctctttcttt atttactaaa catatcagtc gattggatta atccatagca
     4921 aaattctgtt ttacgatact tatgaaataa aagaacatag acctatattt ggtcctatgt
     4981 atcgattagt tataaatgat tatcagcccg atttgatttc tagtttttat tgcaacttga
     5041 tgcaaacaca atctagaaat acagctgtca tagcctttaa aataattcgt aagtccccct
     5101 cctatctatc aaaatgtgac ttgacgagag tgtaaaa
//
LOCUS       DAZKMK010000134         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000134 DAZKMK010000000
VERSION     DAZKMK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.137"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8V1_002692"
     CDS             85..972
                     /locus_tag="ACG8V1_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0330713.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8V1_002693"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8V1_002693"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0330714.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8V1_002694"
     CDS             2274..2720
                     /locus_tag="ACG8V1_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0330715.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8V1_002695"
     CDS             2993..4345
                     /locus_tag="ACG8V1_002695"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0330716.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8V1_002696"
     CDS             4516..4803
                     /locus_tag="ACG8V1_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0330717.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZKMK010000135         4892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000135 DAZKMK010000000
VERSION     DAZKMK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.138"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG8V1_002697"
     CDS             complement(287..649)
                     /locus_tag="ACG8V1_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330718.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG8V1_002698"
     CDS             complement(723..1103)
                     /locus_tag="ACG8V1_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0330719.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG8V1_002699"
     CDS             1185..2360
                     /locus_tag="ACG8V1_002699"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0330720.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG8V1_002700"
     CDS             complement(2437..3021)
                     /locus_tag="ACG8V1_002700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0330721.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACG8V1_002701"
     CDS             3222..4220
                     /locus_tag="ACG8V1_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0330722.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACG8V1_002702"
     CDS             4332..4841
                     /locus_tag="ACG8V1_002702"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0330723.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1553 a          914 c          851 g         1574 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt tt
//
LOCUS       DAZKMK010000136         4848 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000136 DAZKMK010000000
VERSION     DAZKMK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.139"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..332
                     /locus_tag="ACG8V1_002703"
     CDS             141..332
                     /locus_tag="ACG8V1_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330724.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(706..1518)
                     /locus_tag="ACG8V1_002704"
     CDS             complement(706..1518)
                     /locus_tag="ACG8V1_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330725.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1681..1788
                     /locus_tag="ACG8V1_002705"
     CDS             1681..1788
                     /locus_tag="ACG8V1_002705"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330726.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1846..2913
                     /locus_tag="ACG8V1_002706"
     CDS             1846..2913
                     /locus_tag="ACG8V1_002706"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0330727.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            3055..3258
                     /locus_tag="ACG8V1_002707"
     CDS             3055..3258
                     /locus_tag="ACG8V1_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0330728.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            3318..4520
                     /locus_tag="ACG8V1_002708"
     CDS             3318..4520
                     /locus_tag="ACG8V1_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0330729.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1614 a          761 c          998 g         1475 t
ORIGIN      
        1 gttagaaaaa tattattgta gtcttttctt tattaggaat catcactgtt gtatcttatt
       61 accttttctg tttttcttat tagagaaaaa taatttgaga ggtagatagt ttttattgga
      121 ggaagtaatt atgtatattt ttgaaacaaa aatttttcat tgcaataatt gcaacaaaga
      181 aatttcatcg ggggaagagg tttggacaaa aatggaaatt tccgccaaaa gtaactgctc
      241 ctcaactgaa atcaattaaa gaactagaat ttgagaatgc actggtaatt tgtatgacat
      301 gtactgatga agtatttcga aaacattttt aaaggttgag atgtatatag atttaaccaa
      361 tcatttatct ctctcggaaa aggaatatta actaaccagc gggttatcaa aaataggtag
      421 gaaaactgta cttaatggct tatttcgcgc tgattatgag aatatcagtc tgtttgatta
      481 atttttaggg ttcaaaatac agagacaaac tccaggtcgg gtttcgccac aagcgaatta
      541 cccgatctgg agttttgtct attgcaggaa ggcgtagccg gactgcagga ataaagcccc
      601 cggttctaac agggggctaa caaagaacag gaaactgtga caactaccca ttggggagtg
      661 tcctagcagt attttctttg tcacgtgctt atcaattttg tcacctcatt ttgatgatgg
      721 atacgctgca tttaacaggc gttctttctc gacttccatc acgaatggta gataaagata
      781 ttgctgttct agtagatatt gattgtcctc tgtgtactga taagaagcgg tttcttctga
      841 atagaccaac ggggcaattc gcacatcgct ggtcaattga ttaattgagt atagaaggat
      901 ggctttctca cctagttcct tgggtaaatg tttcccaaaa taggaatagg atttttgatt
      961 tgctaaatag acgagtatgc gatccacact aaagttggag aaggtaaagg gcatcatgtg
     1021 cagaatatga ttttgattat catgttgcaa atagagctgg gcttccttac caaaattagt
     1081 tgaaccaaga taaagctgct ttttttcatc gatacattgg aaagtaaact ggtcttgttc
     1141 agttggtgga atgagatttg taataaaaaa ttcaatcatt cgatccattt ctttttcact
     1201 aacaaggtca gcgtgtttga tttcgtctac tatgagacct tcaatcttag gatcaagctt
     1261 acctgccttt ttttggagca tttctaaaat gtattgattc tcttgatcac catacaaaag
     1321 atattcattg gtgacatgac ctatctctgc gatttggtga agggtttcgg tttttggaag
     1381 gttgatccca cgttcccagt tattgactgt acttcgcggt aaatcaccaa ttagtttgcc
     1441 aaatttctcc atactcaact ttaattcttg gcgaatctgc cgaattcgat tgccgactgc
     1501 ttgcttgtct atctccatga aaactcctcc tcaggataag ttcttaaatt tagtatacgt
     1561 gtgtacttaa aaaaagtcaa aacatattga cttaaaataa atacaggcgt acaataaaaa
     1621 tgtacttaaa ataagttcaa taggttcttt gacaagtaaa tagcagaagg aggtttgtcg
     1681 atgaaccaag ttaatcaaca tgatctcggt gaagccattc gagtttctcg agaagaaagg
     1741 gggtggacac aacgatattt agcagaaaaa gtggggattt cgagatagtt attatcgaaa
     1801 gtagaaaaag gaacgagaca attaagtgaa gaaaaattga atcttgtgtt ggatagtttg
     1861 caagaagagg tcgttccagt taaccgtgtt ttgattgatt atctaaccat tcactttttc
     1921 tcgaatcaac atttaaagct aattgaagag attattgaaa tgcccattga acgttttgag
     1981 gaactggatt atgcgccaaa gggctatatt ggccaatacg tgtggaatca agtaatcacg
     2041 attcggtatt ccattgacga tgcggttaaa ggaactgtga tggagttttc aggtcaaggc
     2101 tgtaaacact tagcaagatg gctaaaaaca cgaaaaacaa attggcaaca gttttttcgt
     2161 aaggtattag attatcaggg gaactttaca cggattgatt ttactttaga tgattttgtg
     2221 gggagtctca gcattccaga acttaagcat aaggtaatac taggccatgt gtggaccact
     2281 tttcaagtga gtgaatctca tggcggtacg gatatcataa acaatgaatc aaatggtgag
     2341 accttgtatt taggctcaaa gaagagtcag tgtcgttttt gcttttatca gaaggaccat
     2401 gaacaacgga agcgacgagg aattccttta gaagaggcaa aagtaaagaa tcgctttgaa
     2461 ctacggtatc gaaaagagaa agcgcaaagc ttggcaaaga ttatttcaaa aacccatgat
     2521 ttaaccaaac tgttctttga gttacttaat ggagcaattt gtttttatga ccgtgatcca
     2581 aatgatctag gtgcaaaagt tgatgccaaa tgggcagcct ttattagcaa ttatggcgca
     2641 atcaccattt ctttagatac aattcctcaa agttttgaga aaactatgag ttggttaatt
     2701 catggtgttt caccatcatt agtttttatt gcacaggtcg atcaaacgtt tcattcttcc
     2761 ttgttgcctt tgattattga acaaggagaa atgaatccaa gacagatcaa acttctccaa
     2821 aatatgaagg cagacccgga gtattatcag gaggaggttg agtattatga acgaaaatta
     2881 caaagtatat caaatgagaa aacaagctac taaaaaagca aagtactgct tacacaacaa
     2941 gttgtgcaac gtactccgcc tcgacaagac gagtataaca caaccatcga atgagaaaca
     3001 atagattata aagaggatta ttttaaaagg agtaaagaat caggaggtga gagaatggaa
     3061 acaaaatgtg atttcatggt aaatcgtgca attttgatag agatgggctt taaacctagt
     3121 caagctgcac gaatgattaa agagagcaag gcatatcttg caaggattga aggtattgat
     3181 ttttataata atcgccaagt tggtgtggtg ccatcccgag taattgaaca tctttttcac
     3241 attcaagttg cggaataaac agtacaagcg cggtactatt aaagtagctt ttctcaagta
     3301 cagggaggaa tggaacgatg ccaaaaatta aaaatattta ccaagataaa aagacaaaaa
     3361 aatggttctt ccgagcgtat ctaggtatag atgaaaatgg aagaaaggtt cagaagacta
     3421 aacgaggtta tgcttcacaa aaagatgcca aaattgctta tgacaagtac atggaaacac
     3481 atgattttaa caagacaatt cctaatcaac tgtgttctac caatcaaatg actttcgaag
     3541 agttttacaa ggataggttt gtaaaatggt acgagagaca ggtcaaaagt caaacatatg
     3601 agaatgcgca attcattttc gaaaagcgta tgcagttttt ctatcatttt cgtgttcgag
     3661 atattacaag tcacgatatt gaggattggt tatttgaact aagccagaca gagtcacgta
     3721 attcacggaa aaaagttaat ggcgaaacgt tatcaaaatc ttatatcaat cggattcgag
     3781 ggcacctaaa aattgtattg gatcgggctg ttaaagaggg ggtgattgct aaaaatcccg
     3841 ttgaagatgt ttcttcgctt ccttctgaga ataagaaagt tgagttttgg gaacacagtg
     3901 agtttcaaaa agtgatgaat gaattgaagg atgataagat tcaaacccac catcggaaga
     3961 ttgtgtatga aatgctcttt tatactggca ttcggattgg agaacttgaa gctttgtctt
     4021 ggtcgaatgt agattttgag aaaaacacga taacgattga gaagacattg atttataata
     4081 caaaagatga ttggtacttt tcgactccca aaactaaatg tgcttatcga acgattgggt
     4141 tggggaagaa gctttcctta aaactcagac agtggtatga cttgcaaaaa cgtattggaa
     4201 attttgaata tgttgctcag ttggacggaa catttacgcc accttattct tttgcgaact
     4261 ggttaaaaga agctgctaaa aaagcgggtg tcaaaccgat taagcttcac gcgctacgac
     4321 attctcatgt tgcatttcta attgagcaga atattcaacc gctatctatt caagaacgat
     4381 tgggccatgc gaatattcaa atcactttag gaacttacgg tcacttatat gcaaagtcag
     4441 atcaacaagt ggtaagtgcg attgataatg ttcaagaggg cgctattctt tcaaaaaatg
     4501 acaatttatt accagtctga gttttccacg gttctaccat aagacaaata aatgcttttg
     4561 caccagtgtt ttcagtcgaa ttgctcctgt tcaagatgta tcggaaatag cgagaaaata
     4621 agcaaatttg cataactata ataaaaagga cttagttttg atgggaaatt tttcaaaatt
     4681 aagtcctttt ttatgtggtt gaaacgggta tgatcatttt aaggaagtga tctaagatga
     4741 aaggacgact gaacaattat catattagaa aagctttatt gtattccaca aacaaattac
     4801 taagaataac aaattagttt ttgtttgttc ttattcccta ttgacttt
//
LOCUS       DAZKMK010000137         4755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000137 DAZKMK010000000
VERSION     DAZKMK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.142"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..744
                     /gene="vanR-A"
                     /locus_tag="ACG8V1_002709"
     CDS             49..744
                     /gene="vanR-A"
                     /locus_tag="ACG8V1_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0330730.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            722..1876
                     /locus_tag="ACG8V1_002710"
     CDS             722..1876
                     /locus_tag="ACG8V1_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0330731.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2091..3059
                     /locus_tag="ACG8V1_002711"
     CDS             2091..3059
                     /locus_tag="ACG8V1_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0330732.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3052..4083
                     /gene="vanA"
                     /locus_tag="ACG8V1_002712"
     CDS             3052..4083
                     /gene="vanA"
                     /locus_tag="ACG8V1_002712"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0330733.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4089..4697
                     /locus_tag="ACG8V1_002713"
     CDS             4089..4697
                     /locus_tag="ACG8V1_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0330734.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1470 a          910 c         1115 g         1260 t
ORIGIN      
        1 taatctgata aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa
       61 atacttattg tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac
      121 gagaattata cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag
      181 tctgagattg accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc
      241 tgtcaaaaaa taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca
      301 gaggtagata aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt
      361 cgcccactgg agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt
      421 ggagtaaagg agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac
      481 acccatgagt gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata
      541 ctgcgaatcc tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag
      601 atatggggcg acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat
      661 ttgcgcgaaa aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg
      721 gttggttata aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta
      781 catgtatatc gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat
      841 gatccgaggg aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa
      901 tcacctggac gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat
      961 ttatgtggcg attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc
     1021 aaaatacttt gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca
     1081 aattgagctt tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac
     1141 tctggaaaag cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat
     1201 gtacttggcg cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct
     1261 tgacgaggct ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga
     1321 caaagcgtat cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct
     1381 acaaacgata acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac
     1441 cgatgaattt tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga
     1501 ggatctgacc gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa
     1561 aaacgccgct gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg
     1621 ggatgtggtg tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc
     1681 catatttgaa aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg
     1741 acttggattg gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga
     1801 aagcaatgat aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga
     1861 taaaaggagg tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac
     1921 tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact
     1981 taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt
     2041 ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca
     2101 tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc
     2161 ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat
     2221 ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc
     2281 ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc
     2341 atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc
     2401 cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat
     2461 cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac
     2521 tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc
     2581 ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa
     2641 actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc
     2701 tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag
     2761 catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat
     2821 tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt
     2881 tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa
     2941 tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata
     3001 ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga
     3061 ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct
     3121 gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt
     3181 acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat
     3241 tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac
     3301 catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt
     3361 gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat
     3421 attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct
     3481 gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg
     3541 tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa
     3601 gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa
     3661 atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt
     3721 gccgcgttag ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt
     3781 catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac
     3841 ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc
     3901 ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg
     3961 aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc
     4021 gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg
     4081 tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg
     4141 acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa
     4201 atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg
     4261 ctacccaagg gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact
     4321 gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca
     4381 atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc
     4441 gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg
     4501 ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca
     4561 atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat
     4621 atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg
     4681 atttccccgt taaataaact tttaaccgtt gcacggacaa acggttctgt tgcaaagttt
     4741 taaataaaga ataaa
//
LOCUS       DAZKMK010000138         4729 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000138 DAZKMK010000000
VERSION     DAZKMK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.143"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..1099)
                     /locus_tag="ACG8V1_002714"
     CDS             complement(137..1099)
                     /locus_tag="ACG8V1_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0330735.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="ACG8V1_002715"
     CDS             complement(1101..2540)
                     /locus_tag="ACG8V1_002715"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0330736.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="ACG8V1_002716"
     CDS             2725..4671
                     /locus_tag="ACG8V1_002716"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0330737.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1502 a          811 c          777 g         1639 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca ataaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaggc
     2881 acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaa
//
LOCUS       DAZKMK010000139         4591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000139 DAZKMK010000000
VERSION     DAZKMK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.144"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8V1_002717"
     CDS             complement(<1..300)
                     /locus_tag="ACG8V1_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0330738.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8V1_002718"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8V1_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8V1_002719"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8V1_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330739.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG8V1_002720"
     CDS             1706..1942
                     /locus_tag="ACG8V1_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330740.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG8V1_002721"
     CDS             1949..3901
                     /locus_tag="ACG8V1_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0330741.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8V1_002722"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8V1_002722"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0330742.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1570 a          805 c          756 g         1460 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat a
//
LOCUS       DAZKMK010000140         4560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000140 DAZKMK010000000
VERSION     DAZKMK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.145"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG8V1_002723"
     CDS             <1..792
                     /locus_tag="ACG8V1_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0330743.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG8V1_002724"
     CDS             889..3138
                     /locus_tag="ACG8V1_002724"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0330744.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..4316
                     /locus_tag="ACG8V1_002725"
     CDS             3141..4316
                     /locus_tag="ACG8V1_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0330745.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4319..>4560)
                     /locus_tag="ACG8V1_002726"
     CDS             complement(4319..>4560)
                     /locus_tag="ACG8V1_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0330746.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1578 a          659 c          834 g         1489 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct agttaatttt
     4321 aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa
     4381 cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt
     4441 tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact gtttttcttt
     4501 gttttccaag taagctgtgg tctagttttt ctgatttatc catgttttta cgtggcggaa
//
LOCUS       DAZKMK010000141         4532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000141 DAZKMK010000000
VERSION     DAZKMK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.146"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..328
                     /locus_tag="ACG8V1_002727"
     CDS             <1..328
                     /locus_tag="ACG8V1_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0330747.1"
                     /translation="EFYKSFGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKR
                     SFMKDKDASLVKVIAIMSVFFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKN
                     TKGEIL"
     gene            325..1044
                     /locus_tag="ACG8V1_002728"
     CDS             325..1044
                     /locus_tag="ACG8V1_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0330748.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1059..2144
                     /locus_tag="ACG8V1_002729"
     CDS             1059..2144
                     /locus_tag="ACG8V1_002729"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0330749.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2280..3812
                     /locus_tag="ACG8V1_002730"
     CDS             2280..3812
                     /locus_tag="ACG8V1_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0330750.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            3990..4211
                     /locus_tag="ACG8V1_002731"
     CDS             3990..4211
                     /locus_tag="ACG8V1_002731"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0330751.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1592 a          481 c          879 g         1580 t
ORIGIN      
        1 tgagttttat aaatcgtttg gtaatcttca ttatcagtat tttcatcata tttggttatt
       61 tatagaagtt ggaatattag gagtttcgat attcctattt tttataataa agacattttt
      121 caatgcaaaa agatctttta tgaaggataa agatgcaagt cttgttaaag ttattgcaat
      181 tatgtcggta ttcttttttg tgtataacac aacacttagg ggtgaaacaa cggggccact
      241 gatttatatg attttagcca tgcctgaagt tgtgaaaagg aaagagcatt attatagtga
      301 aaaaaataca aaaggagaga tattatgatt cctaagaaga tacattactg ttggttcggg
      361 ggaaatccac ttccgaaaaa agatagaaag tgcatagaaa gctggaaaaa gtattgccct
      421 gattatgaga ttattgaatg gaatgaatca aattatgatt taaataagaa ccgttatatg
      481 gccgatgcat ataaagaacg taaatggggt tttgttccag attatgcaag atttgatatt
      541 atttataatc atggtggatt ttatttagat acagatgttg agctaattaa gtcgttagat
      601 atacttcgtg aaaatcatgc ctatatggga tttgaagggg aaatctgggt taatggcggt
      661 attggttttg gtgcagaaga aggacattct gttattaaag gtttaaggga tatttacgat
      721 tcattaagtt tttatcaaaa tgatggtaca ttgaacttga aaccgagtcc gtattatatt
      781 acagattatc ttgaatctat tggattggtt cgcgataaca gcgaacaaat aataaatggc
      841 ctgcatatct atcctacgga ttattttgct gccaaggatt atgatactgg aaaaatttat
      901 aaaactagta atacaataag cattcatcaa tataatgcat cttggaaaac acctaaacaa
      961 aaatttgttt atagattaaa atcaacaatt ggacaaaaaa aatttaacag gttacttgca
     1021 ctgaaaaaat ttttacactt ataaaggaga aaagtacttt gaagaaaatt tgtttcatta
     1081 gtggtgtaat ttctcgttct gggggaactg aaagagtagg gagtattatt gccaatagct
     1141 tgtctgattt aggttatgat gtcactattt taagcttttg gaatagtgga gagccttatt
     1201 ttgggttaga taagacgatt aaggttgatt atatgttaaa tcctaaaacg gagggaaaat
     1261 tatatagaac ttatttatat ccggtttgga aattgcacaa gtatatatgc aaaaatgaat
     1321 ttgatatagt aattgatatt gatactttgt tgtctttgta tactacaagg gcaattaaag
     1381 gaacaaagtg caaattgatt tcttgggaac attttaatta ctggacaatg gaaatgctga
     1441 aagaaaagaa gagattcaag gctaaaaaat tgataaaaaa atatgcaaag aggcttgtgg
     1501 ttctcacaga agaagatcgt caaaaacata ttgatgaata tagtttatca gaagattttg
     1561 ttataactat gccaaatccg tgtatatcta atatcgagta cgaatatcat tatgataaaa
     1621 agagatttct tgcagtaggg cgattagtgg aagagaaaaa ttttgctgac atgataagtg
     1681 cttggaaaat tgtagaaaaa aaagtagatg attgggatct tatcattgta ggaaaaggtg
     1741 aattagaaaa agatcttcgc cagcagataa aagatttaaa tctgcaaaga gtaactatag
     1801 cagggcattc agaccatgtg gaggaatatt ataaatctgc atcttgttat gtgcttagtt
     1861 ctgtttatga aggatttcct atggttatat tagaagcaca gtcttatgga ttaccagtta
     1921 tttcttatga ttgtaaaact ggtccgagag atttggtaca tcataatttt aatgggatgt
     1981 tggttgaaga taagaatata gaccaattag ctaagagcat gataatgttt actaagaata
     2041 ctgatttagc aatgagaatg tcactgaacg catataataa tgtacaaaaa tttaatttga
     2101 aagaaattac aaaacaatgg gttgctttga ttgaaaatgt atagtttttt gtggttgtcc
     2161 aaacatgaaa attagcgtaa ttaatcaaag agctagaaga aacctattac atttcaagat
     2221 tttagaaatt atatagtatt aggtaacaat aaaaaataga aagtttggag ccatatgata
     2281 tgataattaa tcgtaaacaa aatgctaaaa ggaatataat tactggaaca atttttaagt
     2341 tatatcagat tatcattccc ttttttatgc gtacacttat gatttatcag atgggaatgg
     2401 agtatgctgg attgaacaac ctgttcacat ctatatttca aatgttaaat ttagcggaat
     2461 tgggaatagg tgctgcacta actgtaagta tgtataaacc tatagcagaa gacaatagaa
     2521 aagagatttg tgctttactg aacgcatata aaaagtactt ttcaattatt ggatggatag
     2581 tgtttacatt aggattgtta tgcattcctt ttttgagatt tttagttaaa ggttcgattc
     2641 ctggaaattt aaatatgaca gttttgtatt taatgtattt gataaatact gttttgtcgt
     2701 attggttatt ttcatataga aagagtctaa tatatgcaca tcaaagaaat gatgtaatta
     2761 acgtgtctat gctaattact tttacattcc aatttgtaat gcaggcatat gttctgattt
     2821 tcttaaaaaa ttactatttg tatttattgg cttctatttt gggacaaata gttcttaact
     2881 taatgtgtgc tgttgtttct caaaagatgt atcctgatta tactcctgtt ggtgagttag
     2941 agaaagaaac tattgacgat attataaaaa aagtaaaagg attagttaca aacaaggtag
     3001 gcggtacaat attacgatct tcagattcaa ttattatttc atctttctta ggtctttcag
     3061 tacttgcgat ttatcagaat tattacttta ttatttctgc cataatgagt atatttgcaa
     3121 taatttatga atcatgtata gcaggtatag gtaacagtct tattgtagaa agtcaagaaa
     3181 aaaattatat agatttttgt gatattacaa caattatagg ttggtttctg gcaattagtt
     3241 gtgcatgctt tattacatta tttcagccgt tcatgattat ctgggtggga agtgataatc
     3301 ttatgagtat ttcatttgtt atatttatga tagttcattt tttcttatat gaaattgatc
     3361 agttaattgg gttatacaaa gatgctgctg gaatatggta cgcagacagg tttagaccat
     3421 taattacagc aatgataaac attgtattaa atatcatcct cgttaattgg ttcggattat
     3481 atggagtttt ggcggcaacc attgtttcag tattgttggt tgatttaccc tggttattat
     3541 ataatgtttt ccaaaatata tttaaatttg aatcggcagg taaatatgtt tggaagctca
     3601 taagtttgtg tgtaatagct tttgttgctt cttatttaag ttattttgct tgttcgtatg
     3661 ttaatatcgg aaatttcaaa gtgataagtt tagtgataaa tttagttata tcggttgtca
     3721 tatcgatttt agttattctt ctgtttaata tccgtaataa gaagtttaag tatattttga
     3781 aagttcttac gtcggtattg gtaaaaagat gagaagagac atcagaatga tatataaagt
     3841 aactacaatt tttaggctgc cgaataggcg aacgatgtat agtttttgtt ccaacgaaga
     3901 ctcttgttga aattggagat gatatgaagg agttacaatt ttgactcatg tttatgattg
     3961 atcggtttta aaagtagtat ggagaagctt tgggatcatc ggagaggatc aaaataggta
     4021 ataatgtttt tataggaatg aagtctagaa tactaaaggg tgtacatata ggaaataatg
     4081 tagttatagg tgcaaatagt ttagtaaaca aggacgtatc agacgattgt gttgttgcag
     4141 gaaatctagc aaaagtaatt atgtccctag atgaatatta taaaaagata aacaatatga
     4201 agaatccgta aaacttgtga aactataccg agaataatat gggaaaaata ttgatgaaaa
     4261 agcattatat gagtttttag ggctgtttac agatggagat gacaatttgt cgaagagttg
     4321 ggagcaatgc aaaatttagt aggggattat aaacagactg acaaggtatt taaagaacat
     4381 gcacaagtat ttcataatat tgaagaattt ttgaatactg tgagatagga gttgctacat
     4441 tgagaaaagt atgtattttt ccaatgtaaa agattacgag tttgaaaacg acaccataat
     4501 attatttttt aagaagatat tatagagctt ag
//
LOCUS       DAZKMK010000142         4462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000142 DAZKMK010000000
VERSION     DAZKMK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.147"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(456..881)
                     /locus_tag="ACG8V1_002732"
     CDS             complement(456..881)
                     /locus_tag="ACG8V1_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330752.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1255..2226
                     /locus_tag="ACG8V1_002733"
     CDS             1255..2226
                     /locus_tag="ACG8V1_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0330753.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2258..3655
                     /locus_tag="ACG8V1_002734"
     CDS             2258..3655
                     /locus_tag="ACG8V1_002734"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0330754.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3814..4437
                     /locus_tag="ACG8V1_002735"
     CDS             3814..4437
                     /locus_tag="ACG8V1_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330755.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1560 a          717 c          911 g         1274 t
ORIGIN      
        1 tactacgtga tgtagaataa gcctttctat cgtttggtta ttttatattc aaatatttga
       61 aataaataaa tctttactct ttgatttgta aactgaaatg atccactgtt tgttttactg
      121 atgaagcttg atcaaaggaa gaagctgagt ttgaagttca taaaaatcag ccatagtgag
      181 ttttgcttgg atttgtttat cttcttctac tttttacata attttgaaca ggataagatt
      241 caaatgtttt aaatgaatct ttgactagtt tgtgacagtt gtcgcatatg ctcaagcttc
      301 cttggtcatt aaattctgta tggatatttt tgttgttatt acatgtacaa taatcaggac
      361 tatcgatcat ccatttcgtc tcctttacaa tcatcttgct tctttactat agcagaaaag
      421 aaggggagaa acaaaagaag atccaacaga cggcactata gactgtagat gttgccatcc
      481 tcatccttca aacggtaatt accgattttt gtaaatatga ctgttgtttc ttttttattt
      541 tcttttcctg aaataatata ttcgtcatct ttggtagtga tcttgatatc tgtcagcact
      601 gttttttctt cagatgattc agaagaatca gttccttgtg tctgatacgt gcgtgtccat
      661 tccatttttt tatctgtaaa tgtcaactgt cccgcggata aatccatatc tttacgtact
      721 tttagcgtat atacactgtt ggtggtatct gctttttccg cttttaagac agaagaatcg
      781 gttgtttcgt ccgaggattg gtcagagccg ccaaacaaat cacagccagt caatgtcaaa
      841 agtcccgcag acaaaataaa tagtacgatt aatttcttca aaatacttcc cccatttgat
      901 aggataaaga aattatacca ttggaatgta aaacttcaaa cggatggaag agaaaaacgc
      961 acaaaacacc cacaaataga gtaaatttta gaataaaatt ttaaaaaggc aaaaaatccg
     1021 ccactttcag aaagcgtttt aaaaaagtgt tgcaatcttt taaaaaaatg ctatattaaa
     1081 aatgaaccgg ttcaaatact tgtttaagta taattaatag tgagaggaag ttgctctact
     1141 gaagacttta gttatatgga tattgatgac aagcaacact atgcaaagaa tgcggtcatc
     1201 ataatcgaac aacaagaaag agaaatcaaa tgtaactggc aggagtgaga atgtatggca
     1261 tcgatccgtg atgtggctaa gatggctggt gtttcagtgg gaacagtttc ccgttacttg
     1321 aatggtcagc aactgaagga aaagaacatg aaaaaaatag cagaagcgat ttcagcgttg
     1381 gactataaag aaaacatcat tgctaaaggg ttgaagaata atcgaagctt ctctattgga
     1441 cttttgataa acgggatttc cagccgtttt gggtcggaag ttgtctcagg gatcgaaaga
     1501 gtagctgaag aaaatgggta tagcttatta ttaagcgggt ttgccgatca gccagaaaaa
     1561 attgaacaaa aaattgaata cctgatgaag catgtgatcg atgggctgat tgtcttctta
     1621 tccgaagaag agtggtctgg atttgaaatg ctcacaaaaa tgagtattcc agttatcgca
     1681 ttgaattgtc cagaaggacc agcaggtatt gatacaatat tagtgaatga tcgagaaagt
     1741 gtagcaaaag tgatcgctca tcatggtgaa caaggacata aaaaaatcgg atttattgct
     1801 gccactcaaa cagactacgt agcaagagaa cgtttagcag gagccaaaga agcggtccaa
     1861 aatgatccat cgatccaatt agaggtcttt acaggagact attctagaaa aagcggctat
     1921 tacggggcaa aagcattatt agaaaaaggg gcaactgcta tttttgtcag caattacaat
     1981 atgtcgattg gtgccatcga atgtttcaat caagaaggtg tcagaatcgg gaaggatatt
     2041 gcgttcagtc actatgatta tttagataaa aacgatctga gtatccttcc gaaaatcacg
     2101 atcacgccac caacagataa aatcggggaa cgagcagcag aacagctgtt aaaacgaatc
     2161 aaaaaagaag aggaagaaaa tgaaaaagtg attgtgatgg aaaatatcat acatggaatc
     2221 aactaaaacg aactaaaaaa gaaaaagagg tgctttgatg aaaaaatatc aatttccagc
     2281 aaatttttgg tggggttctg cagcaagtgg cccacaaaca gaaggaagga tttcaggcga
     2341 tggaaaagga gagaacattt ttgattattg gtacaaaaaa gaaccgcaaa agttctttga
     2401 tcaagtagga cctgaaaaaa cctcgcaagt ttatacgaag tatcaagagg atgtacagtt
     2461 gatgaaacag actgggcata attcatttcg aacatctatc caatggagcc gtttgatacc
     2521 tgatgaatct ggaacaatca acgaagaagc cgttcgtttc tatcatcgtt attttgatga
     2581 gttgattgaa aacggtatcg aaccctttgt gaatctgtat catttcgata tgccgatgta
     2641 tctgcaagaa aaaggcggat ggttgaaccg agaaacggtt gaagcctatg aaaaatatgc
     2701 caaaagttgt tttgaattgt ttggttcgaa agtaaaaaaa tggtttactc ataatgaacc
     2761 aatcgttcca gtcgaagcag gttatttata tcgatggcat tatccagaag agtcagatat
     2821 gaaaaaagcg atccaagttg gctatcatga agcgttggct tcagcgttag ccatcaaatc
     2881 ttaccatgaa atggagcaag gtggtgttat tggtatcatc ttgaatctga caccaagcta
     2941 tccaagagac gaatcaaacc ctgaagatgt taaagcagcg caaatagcag atgcgttctt
     3001 caatcgttca ttccttgatc cagctgtaaa aggcgagttt ccaagtgaat tgattgagat
     3061 agtaaaagaa cttgatctca tgcctgtgat ccatgcagag gatttgacgg ttatcaaaga
     3121 aaatacagta gatcttttag gtatcaatta ttaccagcca cgccgggtaa aagcaaaaga
     3181 aacaccaatc gatactaaaa acggtccaat gcctgaggat tatttcgaca attatgaaat
     3241 gcctggtcgt aaaatgaatc cttatcgcgg atgggagatc tatgaacaag gaatctatga
     3301 tattttgatc aatgtacgag atcattatgg aaatatcgac tgctttattt ctgaaaacgg
     3361 tatgggtgta gaaaacgaag cacgatttat caaagaagat ggcatgatcc acgatgatta
     3421 tcggatcgaa tttgtccaat ctcatctgaa atatgtccat caagccattc aagaaggtgc
     3481 caattgttta ggctatcata tgtggacatg tatggataac tggtcgtgga ccaatgcata
     3541 taagaatcga tatggcttta tttcagtaga cttagcacaa gatggaaaaa gaacaatcaa
     3601 aaaatcgggt cattggttca aagacgtatc agataaaaac ggatttgagg cttaagaaag
     3661 aatagttgag agcaaatgcg tgaaattctc cattcttacc attttgtgaa aaagaagttc
     3721 aattatttac gatctttttc aacttttttg tctttctatt tcattttttg tatataatag
     3781 aatagtcaaa attaaatagg agtggaggaa agcatgagga aaaaaagacc gtttcttctc
     3841 ttttggttat tcggtttttt cttactgctt attttatccg cagcattttc aggaaaaatg
     3901 acagcagatc ttgctcagaa agaaacagaa aaaatcgtag caagcaaaga atcagtagat
     3961 aatttcaaaa aagaattctc aacttattgg tgggcggagt ataaaataac tgccaattac
     4021 gaaaaaggcg aattggacat ttatttgcca aaaggaaaag aagaaaatca atttattaat
     4081 atcgatgacg tacttagtgc gatttctctc agacaatatc aatttaaaga caagcatttg
     4141 accgttttgc ttttgactca tgatggcgac aaagtagccc gtttgaaaaa tggaaaagtg
     4201 atcaccgctt attctgaaaa agtagacaaa aaagtggtcg ccgatcgttt agcggaatgg
     4261 aaaaagacaa atgaacaagt caagaaaaca actgtctctt ctcaaaaaaa cgagtcgaca
     4321 ggaacaagtc cttcagattc tggcagtgga caaacgataa cgggaagtga agaaaatcaa
     4381 gcctcaatta ttacgaatga gtcatcacaa ggacaatatc aatcctatgg acaatgaaaa
     4441 acagaggttg tgaaatcagc gt
//
LOCUS       DAZKMK010000143         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000143 DAZKMK010000000
VERSION     DAZKMK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.148"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG8V1_002736"
     CDS             690..1658
                     /locus_tag="ACG8V1_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0330756.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACG8V1_002737"
     CDS             1726..2484
                     /locus_tag="ACG8V1_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0330757.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG8V1_002738"
     CDS             2552..3262
                     /locus_tag="ACG8V1_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0330758.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG8V1_002739"
     CDS             3298..>4425
                     /locus_tag="ACG8V1_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0330759.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZKMK010000144         4377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000144 DAZKMK010000000
VERSION     DAZKMK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.149"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8V1_002740"
     CDS             73..234
                     /locus_tag="ACG8V1_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0330760.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG8V1_002741"
     CDS             253..1530
                     /locus_tag="ACG8V1_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0330761.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACG8V1_002742"
     CDS             1540..2196
                     /locus_tag="ACG8V1_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330762.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACG8V1_002743"
     CDS             2196..3572
                     /locus_tag="ACG8V1_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0330763.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACG8V1_002744"
     CDS             3702..4115
                     /locus_tag="ACG8V1_002744"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0330764.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACG8V1_002745"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACG8V1_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAZKMK010000145         4325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000145 DAZKMK010000000
VERSION     DAZKMK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.150"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            446..592
                     /locus_tag="ACG8V1_002746"
                     /pseudo
     CDS             446..592
                     /locus_tag="ACG8V1_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(680..973)
                     /locus_tag="ACG8V1_002747"
     CDS             complement(680..973)
                     /locus_tag="ACG8V1_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0330765.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1010..1195
                     /locus_tag="ACG8V1_002748"
     CDS             1010..1195
                     /locus_tag="ACG8V1_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330766.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1376..2554)
                     /locus_tag="ACG8V1_002749"
     CDS             complement(1376..2554)
                     /locus_tag="ACG8V1_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0330767.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2658..2972)
                     /locus_tag="ACG8V1_002750"
     CDS             complement(2658..2972)
                     /locus_tag="ACG8V1_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330768.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3079..3294
                     /locus_tag="ACG8V1_002751"
     CDS             3079..3294
                     /locus_tag="ACG8V1_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330769.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3594..3836)
                     /locus_tag="ACG8V1_002752"
     CDS             complement(3594..3836)
                     /locus_tag="ACG8V1_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330770.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3823..4251)
                     /locus_tag="ACG8V1_002753"
     CDS             complement(3823..4251)
                     /locus_tag="ACG8V1_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0330771.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1370 a          827 c          649 g         1479 t
ORIGIN      
        1 aaaatactct ttttttacga ttattgaaaa aaaaagataa aacttgatta attgaagtat
       61 tgcttaaatt tggaagctat gagggaagaa taaaagagtt aggacgggat tatctactat
      121 ttttctttgt tttaaatatt tgttatcttt gaacataaaa gagaaatgtg tcgactgcag
      181 tgttgtgtcc ctcatcacga ataaacggtg ttcaactagc tgatcttgta ttcacttaaa
      241 aaatggtaaa gtacgagttg ctattcccca ttttcaattt tctaacgctc aggacaaaat
      301 actgttttca caacctcatt ggaataaaag catatatatt gtttcaagaa cgaaatcttg
      361 ttatcttttt aaagggcatc tgtccaaaat ctaattttag gaggtacgtt ttggtattta
      421 ttcgtacaaa taaaagagaa ttatctttat tcatgtaacg catccaattg attttttcat
      481 gtacgctcct ggaacgattt tactgacgat tgcttattta actgcgaatc gctcattagc
      541 ttttatcata gctatccata ttttgaataa cgttcttggc tttgtattat agatacagat
      601 gacaaaaaaa gttcaagtat gcggtgtaga accaatatcg catacttgaa cttttttcaa
      661 tgagtataaa ttaaaacttc taattttcaa ttcccatcat ctgatatgcc cgaccgtctg
      721 aacttctttt gaaaaagcca aatgtgatta gataacgtct ttgttcgaca taatcgccac
      781 tagaaaatac gttcagcagt atcgaattaa cctctgtttc tgtataagtc actccatggt
      841 caaagcgttt caagacttct ttcaatagga tgtatttctt ttttgttgaa cttggtactt
      901 gttttacttt tccatcattc atgaatgtgt ctaaaacttt catcttttct tgctccgtat
      961 aagtataaac catcttcttt ctccttttga ttctaagaat actccattta tgttagaata
     1021 ttctggattt tttattaaag cggttactct taagaaagat attatcatag tgctcttttt
     1081 tttactacta cttataggta gatttattct aatccattat tctcccctac caataattaa
     1141 agaaaaaact ggatcagggt gttcaaatta catgctaaga aaaaagaacc cttgaatacc
     1201 aagggttctt tctcagtctg ataacaatga ttaacgacgg atttctttga tacttgtgaa
     1261 aagtcgttaa agtattgata taaaaggatt tttattacct ctgactacat attgactaat
     1321 ttgttaaaaa atcgtttttc aagaatcctg taataccagt gctttcaccg tttttttaac
     1381 tctctaaaga actaaaagca atcagaggtt ttaccttttc atttgacgtt aagtggcgat
     1441 aaattttcga agttgtttgg tcagtatggc ctaagcgatc tttcaagact tcccagctac
     1501 agtgttccgc tactaagctt gccattgtat gacgagctaa atgagtatga aatggcttat
     1561 taattcctga gcgattcacg caccgtttga cgtgatcatt tacatactct cgacgataag
     1621 gaacaccacg tttactagaa tcaagataca caaacaaata tgtttcttct ataataaacc
     1681 tgtaatgttg cctcatctca cccaaagcct taatcaactg ttgtagcttt tccataacta
     1741 gaggagttac tagaactcgt ctagcagaac taactgtctt agtagtttca attcgaaaat
     1801 ccttactctt acctttaact gccgtttgct tatagaacag aatttcattt cttttgaaat
     1861 ctatatctcc aggctgtaga gcaagcgcct cgctgactcg gcagccagtg taaaatagaa
     1921 catggaaaag tacagacatc ggtaattcct catgaacaat gccataattc aaaaaagttc
     1981 tcaattcatc gacagtaaga aacttgtcct caatattatt tatggcttct tttaaatcag
     2041 ttaccgttaa ctgatatttt ggcattctca cagacttcat tggattttca cttatcaaat
     2101 tctttctttg acaatattta aaaatcatat ttagaacttg aataagtgat tgtaaactat
     2161 ttttggcata gccaacattt gttccattaa cgccacattc ctttaaggta aaaagcaatt
     2221 cttgaaggca ctctggtgtc aaatcagaca actttttatc accaatccat cgaacaataa
     2281 tttttaattg ttcctctcgc ttatcatagg ttatttcctt ttgttcattt aacgaacggt
     2341 aatattggaa ccattgctca cctacaacac taaacttgtc attgcttgca acaatttttt
     2401 tcttaagcct ttgttgtttc tccatttcca gataagcgag ttgcgcctca cgttttgaat
     2461 taaagccacg tcttgttgtt cgatatgctt gaaagtacca tgtgatccta ccagtagaac
     2521 tgatttgctt cttgaatcga tctgtttttt ccatttattg attcctccta ttttgaagga
     2581 acccaacaag cagaaaccct ttggaaatct ttctatctat gtcatcattg taccgcatct
     2641 tagaaagaaa atgtaactta taaagatcga agtagtcgca atttttctcg atagtcattc
     2701 gaatcactct ttctcatcca ttctaaaaca tcatcacgaa aatagtgtcc tcgaatcccg
     2761 taccaacagg gaggaaactc accaagtttt actaaacggt tcagtgtact atcacttatc
     2821 tgaaatagtc gctttaaatc agtatttgtt aataccagag gtaaatgact gactgcaagc
     2881 aatttttccg tgtaatcttc cagttttttt tgtaaaattg aatagaactc tatttccaga
     2941 gaattaacat ttgttttaac ttccaactcc atcacccttc tctctcaaaa tcaaaacgac
     3001 caccacggta ccccttaaaa ttgtaaaaaa acactccagt actccatcaa aacggactag
     3061 aaaagaggaa tttttaatat gaaacaagga attgcagaca taaaaataat taaagaaatt
     3121 cttgaaaaat ctacggctaa tgcaatagca ttcggaacag gaatcaactt gagtacagta
     3181 aaaaaactta aaagtggaga gagagctgaa gaaaaattaa atcttgccga tgctattaaa
     3241 atcacagaat ttggcatgaa aaatatgcca accaaaatcg aaatatggaa atagtagttt
     3301 ttctgatcta tcagctgttt tgccaaagca cataggaatc tcacctcccc ccagtaatgg
     3361 cgggccgatt tggttacgga agtatcatta tcgtagtcgc gattctcata ttaaaatttg
     3421 tcgcttaaaa gctaaccttg tcatggcgta actagagaat caaaatctac atgtactgat
     3481 agatttgcta ttcatttgtc aaagagcgct aggctatttt cagcctatgg aaatccttta
     3541 ttttgacccg ataaaaaact tccatagaat gaaaaggaaa gggaaaaaat actttaacga
     3601 gttgtatcaa attcaagtat cttctcaatc aattttgttt ccagttgtct acgcatatat
     3661 tcatctacat acataaccat tcctttttct tcttcataaa ctggtttcat ggctaaccgt
     3721 gaaatataat tttgatattt tgacagaacg atggacatgg caattgaatc accagaagct
     3781 gcggctttga ttgtcgttag tggtatgaat ggtatatatc gtttattttg cttcatattt
     3841 gacacccaaa tactttccta gctcttccaa agccatttgt cgttggtacc aaacactgcc
     3901 gagacttttc ccaataatat cgccaatttc acgatcatta agaccgatga agaaggacat
     3961 aaaaatgatt tcttgtcttt gactaggtaa tgctgcaatc gcatttgcca actcctcatt
     4021 ggaaataaag attttcatac caaggacaat aaaagtacca ctactattca aatttggttc
     4081 caaataatag aactctgtac ctaacccttt ttgttccaat atttccattg atatttgccg
     4141 ttggcgaaat ctagcgtact tcttttgaat atttaacgcc tcattgcaga ctactttctt
     4201 acaaaaactg tcaaacgtat gttgaatgtg ggtttcctct gagtcgccca taatattctc
     4261 cccctcaaaa aattcattca actcgtttta cttctatccc tttccattat tactacaatc
     4321 tagga
//
LOCUS       DAZKMK010000146         4317 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000146 DAZKMK010000000
VERSION     DAZKMK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.151"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..554
                     /locus_tag="ACG8V1_002754"
                     /pseudo
     CDS             117..554
                     /locus_tag="ACG8V1_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            649..1533
                     /locus_tag="ACG8V1_002755"
     CDS             649..1533
                     /locus_tag="ACG8V1_002755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0330772.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1755..>4317
                     /locus_tag="ACG8V1_002756"
     CDS             1755..>4317
                     /locus_tag="ACG8V1_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0330773.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1411 a          722 c          964 g         1220 t
ORIGIN      
        1 ttagttcttt ttattttaaa aatttttttt acttagaatc cagccaccct agatattttt
       61 ctgtataatc ttaaataaag aaaacgatat taaaaaatgc acgtagaagt agataaatgg
      121 agtggttttg caaatttctt ttatttctag aagttattca cgtatacgga tttgtttttc
      181 tcacaaataa aaaggcagga agtacaatgg atcatttgta tataaaatat attcatcaat
      241 tttccgtttt ggtattaggc acactaatta tagcggggat tgttgcattt attattcaag
      301 gtcgtgacat attaacagta catcaagagt ttttcagtta tgcatttggt attcctctga
      361 tcgctataag gtctgataac aaaaagtgga agaaacgcta ttacattctc ttgctatttt
      421 tgtgttttta tctgccacct aagattttta ctttattagg tatacaatgc ctataatttt
      481 cttttatttc ctatatcatt gttttaattt tcctcagttt catagtatgg gtattttatc
      541 ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca tcacagccta atttttttct
      601 ttatgctatc gttatattaa agaaatcaat cagaagggaa agaaagtgat gaaaaaagtt
      661 ttattagtgg taaaccccag ttcaggcggt gaacaagcaa aggaatttga gcaattagcg
      721 atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct tgcatacaaa aaaagcaggg
      781 gatgcaaaaa actttactcg cgaagcggct acggaagggt atcatagtgt gtttgtcatg
      841 ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg ctgaacaaga acatcggcca
      901 aatttcggat ttttcccatt gggtacggtg aatgacttgg ctcgagcatt agggataccg
      961 ctagaacctg aggaagcgat caatcatttt tcgattgaat cagtcaagcc attggatatt
     1021 ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa ttggctctat ccctgaagca
     1081 atcaatgatg tagatgcgga gaagaagacg aaatttggaa aactagctta tttcatgtca
     1141 ggaatcaaac agttagcttc cacacaatcc tactcgtttc acgtagaagt tgatggaaag
     1201 aaggaagaaa ttgagagcag tatcttgctg ataggactaa ctaactcagt aggcggtttt
     1261 gaaacattac ttccaaatgc aaaagtggat gatggtaagc tccatttggt ttatttaaaa
     1321 gattcttctt tgatcgatac gcttaaaaca ttgccagacc tattaaaagg agtagatgaa
     1381 tctacgaaaa atctagccta tcttacttgt gagcaattag ttgtgtcatt agctgatgag
     1441 gaagaattga cgacgaatgt tgatggagat gaaggggata aattacctgt gacaatcaaa
     1501 gtgttaccct cacatttaca ggtttattgt taaaatggag gttgtgaccg aagagttgtc
     1561 aggaggacat cggttgttcg gaataaggga ctgtgacaag acttttgtca cagccttttt
     1621 ttgtacaaaa aagaaatttt cacctaacag aaaaatttgc cataaaaata catattttaa
     1681 atatgaaagt acaattacaa ataaattaaa acgttaaaat aaaattgtca aaacgtcaga
     1741 gaggatggtg gagaatgaat caatatgtaa aaaagctgat tgcgtcgatt ttgtttgtct
     1801 tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaagc gaatcaacgc
     1861 aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcaatt acttctgaaa
     1921 ctacagaatc ccaaagtgag gcgcctactt ctaatacaag gcaagaagaa aataagacaa
     1981 gtgagacctc agaagtagct gaacaaacgt ctgatagcag tgaagaggta caggttagag
     2041 ttgtttcagc ggatgggtac agcgaagctg ccacaacaga agaactgacg cagcttctcg
     2101 cagacgaatc agtagctaag attcgtttaa ctcagccttt gactttagac agggaattgg
     2161 agatcaagcg agatatcgtt atcgattttg gcggacttgc tcataatttc ggcacacatc
     2221 acatctatat caatgagaca cctcagtcga tcgagtttca gaattttaaa ggaacggcag
     2281 ctcatccagg tgggttgata ccaaatatcg atggaaatgc cattatcatt gcttatatgt
     2341 ccgatttttg gggaaacaga tatcatttta cgggagaaat aaaatttacc gggacacttg
     2401 acttatatga tggttctaaa ttaggcctga tttacgcccc tcgtgcaact gtcacattag
     2461 atggtgtttc cggtgtattg gatgtccaac cagtgaaaga agggatgaat gccgctccgg
     2521 gtaaagctta tttttgtaga agctatcagc taaatgtaat caatggtagt cagctttatg
     2581 gaccatatct aggaaaattc tatggttttc tagattcagg tgatgagacg ggcagtggaa
     2641 aaacagcacc aggactccat atcttgagtg gttcaaaggt ctctcttgac tatgatcggg
     2701 cagatggcgt cagcgatgaa ggagaggctg tcgatacgct acccgggaat gtcacattta
     2761 aagtcagtgg ctcaggaagt gaattttcgg taaatacaaa gatcaatatt accaacgaca
     2821 acaatcgcgg aattatccaa atgagaggct caggatcaag agtcgctgtg tcgaatgatg
     2881 ggaaaatcac aatcaataca gcgactactg gcggatttcg cctgcaagga gattctagtc
     2941 aaatccatgt atcaagtggt ggacaaatct cggtcaaaat ggctggagat ggcgaccaac
     3001 ctggaaacaa tgggatgcgt tttgtaggga aaggactttc tttgatcgta gaaggagccg
     3061 gatcagtcat cgatattgat aagcaaagtg gtcgttctag cgcagttcgt tttgagaacg
     3121 gtacgcaaaa actaacagta actaatggag gctcattgaa ggtaagaaat gcaggggatg
     3181 gcaaatctta tgtgaacaga ctcaatcaag gaaaccaagc gatccaattt tatgacgctt
     3241 ctggttttct agaaagtcca gggtcagctg attttacggt tactggagaa aaatcaaata
     3301 tcgatatacg cgcagattcc ggagctaccg tagatacaac tggagatatt gatttgaact
     3361 ttatggctgg cgaaaaaact tatttagtca tggtcgggaa aactggggaa gataagactg
     3421 gcatcatcag tggggatgtg ttgaatgtag acttagaatc tcctacctat ttcgatttcc
     3481 aaaacaaacg gatcggaagc gagactaatg gcataggtgg ctgggtattt caaggaaatg
     3541 gcgattcttc tttgaagatc gctcattcag aaattgcttt atggcgaaag gcaggagtaa
     3601 cgttcgataa ctttgaaaaa gatccggatt atttttctga tatgacagac ttatcgatca
     3661 atggaaccga cttaggtaat tttgtatcaa gccctagtgc aggtttaaca aatgaatttg
     3721 ctcagacagg tgccggaatg aaaaattata atcgaatcag tgccaataac cagcttccga
     3781 tgattgaatc cttaagagta ccaacgaatg cagataaaaa aatctatggg catgtcgttg
     3841 ttcccgaagg tgtcgaggca aagccaagag atgcttggac aggcgaagta gaagtgactt
     3901 tacgtattac ttatgcagat acgacgaaat cacctgtaga aattactgct ctcacacaag
     3961 gacaaacaga tgaaaatgaa gggacaggat ataatccgtg gggagaaggc gaacaaggcg
     4021 gtttgtttga ggttcaagta cccaacgaag aaagactaac aacgggggat caagtgcaaa
     4081 tagttgctgc taaaaaagta agaggcggcc aagaagtgac agaactggac tctgccaaaa
     4141 cgacagtcga tgtgattcct cctgagcctg ctaaattaag taccacattg atcaatgcag
     4201 cttctcgctc aatcacaggt acaggcgaag caggagcttt ggtttcattg aaaaaaaatc
     4261 aagctgtttt gtccgaaaca actattgatg atacaggtaa atttgttctt tctatcc
//
LOCUS       DAZKMK010000147         4157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000147 DAZKMK010000000
VERSION     DAZKMK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.153"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1383
                     /locus_tag="ACG8V1_002757"
     CDS             82..1383
                     /locus_tag="ACG8V1_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0330774.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1410..1670
                     /locus_tag="ACG8V1_002758"
     CDS             1410..1670
                     /locus_tag="ACG8V1_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0330775.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2669..3904)
                     /locus_tag="ACG8V1_002759"
     CDS             complement(2669..3904)
                     /locus_tag="ACG8V1_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0330776.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1192 a          669 c          866 g         1430 t
ORIGIN      
        1 taattatata attagttatg aacattcttt gcgttgtcat gcaatgaatg gttcatattt
       61 tattgaaaag gagtggtttt agtggaaaag ctgatgaaat gggtcgaaga gaaacttgtc
      121 ccaccaatgg caaaaattgg tacacagcgt catttattag caattcgtaa tggggttgtt
      181 tcaacattgt cattgatttt gattgggaca ttttttatgg tctttatcaa tttaccgttt
      241 cctggatgga atgaatttat tgccccttat tcggcaacga ttgttttacc ctttaggatg
      301 acgatgggat tgatggctat ttatgcaact tttgttatgg gttcggatct agctaaatca
      361 tatggattag atagtgtgac aggtggtata ttatctttag gaacgttctt tatgttacaa
      421 gttcctgtaa acgttttaac tcctgaagag gcacctcttg gctgggtctt acctatgagt
      481 tctttgggag catctgggat gtttgcgggg atcttatcta tgatttttgc agtggaagta
      541 tatcgtttct ttaaaaatcg aaatattact atcaaaatgc cagaacaagt gccacctgct
      601 gttgcccgta gttttgaggc attgattcca ggtgcagtca ttttaacaat tacttggctg
      661 attcgaagcg tcgcgggctt tgatgtaaac gctgccttat tatcgttatt cgagccttta
      721 accaatattt tagggaataa tttgcttggt gttttactac caatgttttt gatccatttg
      781 ttgtggtcat ttggtatcca tggcatgagt attgtagggg ctgttgttcg tccaatgtgg
      841 ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg gtacagctgc cacccaatta
      901 ccctatatcg ctcctgaaca gttttatcaa tggacggtaa caattggtgg tgcaggagca
      961 acgatcgtgg tatctgtttt gtttatattc ttctgtaaat caaaattctt gaaagaggtt
     1021 ggtcgttttt ctattattcc tggtatcttc aatatcaatg agcccataat ttttggagct
     1081 cctatgatgt tgaatcctta catgttcatc ccgttcaact tagttccttt agttttgact
     1141 attatcagtt attttgcagt caaattagaa atggtcaata gatttactgt tttgccaaca
     1201 tggacattac cagctccaat cggcggttat ctttcagcag gaaatgactg gcgtgttgtt
     1261 atcttgatca tgatcaacac attgatagca tttattatct attatccatt tgtaaaagct
     1321 tatgataaaa agatgctgtc agatgaacaa agtcgtggag aaaacgaagt ccagactgtc
     1381 taagtttggt ttttagaagg tgggaaacaa tgtattggct gattttattt tttgttttta
     1441 tctttttatt aacggctagt caccttatat tgaatatgct agctacatat catattcaaa
     1501 tcaatcgatg gatttgggca ctggcatcat ttttgatcgt gattcttcca aagatcattg
     1561 taccgcatat gaatgttttg ttttcatggg ggacatatgt actttgtgga atttttgcta
     1621 tcaattttat gatcgagcag catagatggt ttgtcacatc gaagctttaa atgttctaaa
     1681 agtggttgta acagaagtgg ttagcttcaa gaaataagaa gaaatttacg aaaattgctt
     1741 cttaaatttt tgtgaaatgt tggcttattt ttcgtttggt tgaacttatt accgcagatg
     1801 caagcttttg accaccgttt attcggaata tgggactgtg acaaggcttt tgtcacagcc
     1861 cttttttaca taaagattat tattgtttta actgttttgg cagaaagaag gaggaaaaaa
     1921 tggaaatttt atcagcaatg gatttagaat tagtagatct actaccaaag tgatcaagtc
     1981 aaatatagat ttgggagctt acaaccatat agtaggggct gcagtatggt gtatgaatgg
     2041 taatatgtat gatgtgagtg tttattcaaa tcatgaaacg tgtatagaaa taattgtgct
     2101 gggaatagca atcagtcatg gtgaacgtga cttttagtgt atcgttgctg acgacataga
     2161 acattctgac atgatctact caatatgtgg ggattgctgg cagtttatat aggagtatgc
     2221 atcggattgc gagatcattg tctcaacaga agagggaggt tgattatcaa agataaagtg
     2281 gtaaatccta catttgattt agtaattgtt gaggggacta gcggcacaaa tctctacaaa
     2341 gcgatgcttc tagatctata tgacacattt taagaaaatg tatttgccta attattatgg
     2401 tctttctttt gaagaaacgt tgagatgatc caaccagaga aaggtgtcaa agaatttgac
     2461 taagaataaa tggcagacgc tacctatgct catttggata gcgtcagatc ttttatttta
     2521 aggatgattt tgtgtttttt agacaccgta atttaagtaa ctctaacttt tcaatccact
     2581 atttactttt tttgtatttt cactgtctgg gtctcgaata gcgaaattac tgatgagtct
     2641 ctattaaaaa agctttttct acttgaaact atattgggtg gttgctcagg aaggtttcga
     2701 tttcttgaat tgcttgttgt ggaccattta ttggaaaaac ggcttgcagc gtgcgtgcct
     2761 gctgacgaat cttgggtgaa gaaacttcgc gcagagcaat ctgcagtttt ctttcagtca
     2821 ctggttttgt gagggcgatt gcgtttccgt attttacaca atagtcaata ttacagcttt
     2881 gctcaaattg catccccaca ccgataaacg gtttgccgga ttcacaggca gtttggacgg
     2941 tcccttcccc gccgtggata aaagaaaaat caatgaattc atgcaaaaga tgactagaca
     3001 gatattctgt tattaaaaga tcttctgagt cagattgaat gtcatcaagc agatagtcaa
     3061 taggtgcaat aaccgctaga tccggttgtt tttgtaagaa gaacaaaatt ttgcgtaaca
     3121 tacgcggatt gcctgaacta cccattgcga aatagataat acgttttctt tcttgttgct
     3181 ttcgctgtat aaaagttagc acttcttcag gcaaggaagt actgagctta gcaaaaattg
     3241 gaccaacgat tcgataattt tccggcagct gttcttcttc caaaaatagt ttaggcgttg
     3301 tgatgagatt tagatcagca tccatcattt gtaaacttgt ttttggtaaa ggaagcattt
     3361 ctttttgtgc agcgtgtttg aaggcattgg ggatccatcg ggtatttact atacgctttt
     3421 ttgtccagtg ccaaagggga ttgcttaaag ggccgaagaa acgtaactcg tggggcatca
     3481 gcgcatgctc tgattgaaaa aaaggttcac tcaaagcata gggtttaaca tatactaaag
     3541 gaatcttttt attgcgggcc gataaaaaaa ttgtcacatt cgaacctgtc acaatcagtc
     3601 gaggctcaaa cttagcaatc agactctgtt cactagtgat acgagaggca accatttctt
     3661 tggtaaaagg gtttttaagt gtacgcaact ggtcaaattt tatgatttgt tcttcttgtt
     3721 ttttcgtaag gatcggcgct agtaaatgat actcaaagcc actttctgtg atcaaaggag
     3781 caaaagtttt agaaaagcca aagaatacac agtaataacg gtctttcata gctttggcaa
     3841 cctcaatcat tcgtgttgtc tcagctaaat tgaatactgc aggagcaaaa agaattgtct
     3901 tcatgaaatc ctccttattg gaatgagtct atcccttcgt ttcttactag tatactaaaa
     3961 aacacagcgt tcgagtagcc tatgtatatt ttgaatatta aactgtctct cgctgttttc
     4021 acaacttcta aatttaaatt ttgtaatcaa gttgaaactc caattttcaa aaaaacacgc
     4081 aacaattttt tgatcgattt atatgttgtg ccagataaat acgagaagtt gtgtcattaa
     4141 aaataaacat gagaaca
//
LOCUS       DAZKMK010000148         4139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000148 DAZKMK010000000
VERSION     DAZKMK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.154"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..1495
                     /locus_tag="ACG8V1_002760"
     CDS             71..1495
                     /locus_tag="ACG8V1_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0330777.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1676..2449)
                     /locus_tag="ACG8V1_002761"
     CDS             complement(1676..2449)
                     /locus_tag="ACG8V1_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0330778.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2446..3351)
                     /locus_tag="ACG8V1_002762"
     CDS             complement(2446..3351)
                     /locus_tag="ACG8V1_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0330779.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3505..3825)
                     /locus_tag="ACG8V1_002763"
     CDS             complement(3505..3825)
                     /locus_tag="ACG8V1_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0330780.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1253 a          758 c          824 g         1304 t
ORIGIN      
        1 aattaagtaa aaataaaaaa ggtttgttat actttttgat aaaagcggtt ttattttgga
       61 ggatgcttac atggatacag agcaacagaa aaaaggaatt ggtttaattc cacttgctgc
      121 cttagtaatt ggttcagcga ttggaggtgg cgtatttggt attatgacgg acatctccgg
      181 atcggctggc ggcccagcta ttttcagttg ggtactcgta ggaacagcaa tgttgatgct
      241 ctccttgtcg atcaataata tcaataaaaa acgtccagat ttaaaaggcg ggatctttag
      301 ttacgcagaa gaaggttttg gccgtttcgc tggttttatc agcggttggg gttactggct
      361 cacatgctgg ttaggcaacg tagcttttgc aacattgttg atgagcgcat taggatattt
      421 cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc gtgatgagta cagtcttttt
      481 atggggatat gcatggctag tgaatcgggg agtcgaaaat gctagtttcg tcaatgctat
      541 cgtcacattc tgtaaattag tgccattatt tttatttatt gtagtagcta tcagcacgtt
      601 ttcgatccaa caattcattg ataacttttc tagcgacatt atattgaatg cgaatggaca
      661 gccgatcccg ttttccagtc aagtattgag ttgcatcatg gtgatgcttt gggtatttgt
      721 tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa aaacaatcag atgttgggaa
      781 agcaacgatt ctagggatat tcggcttgat tattatttac gtcttggttt ccatgcttcc
      841 atatgggaca atgacgatga atcagctgca aacgatcaag gcgcagcctg ctatgggcta
      901 tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg atcaacggcg gtttgatcat
      961 ttctttagtt ggtgtttggc tctcatggac gatcttaccg attgaaacaa tgggaaacat
     1021 ggctgaagac ggacttttgc ctaaagcatg gggaaaactc aacaaaaatg gggcaccaac
     1081 ttatgctatc gtattgacga cgttatgtac gaatgtcttt ttactttcat tattattcac
     1141 tgacagtgct tataactttg cttacacact tggtacagca gctattttct tcacgtggct
     1201 tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg aaagaatggg tccaattttt
     1261 agtagggctt ttagcaagtg cctttcaagt atgggctatg ctagtcgtag cctttaatga
     1321 agtaatgctt gttcttgttc ttttcttgcc aggcatttat ttttacctca aagcacgtaa
     1381 ggagcaaaac gagcctgtac aacatatcca cttagataag attttactta taggtgtgac
     1441 gatggtcgga gtgattgcaa tggttttatt tgccactggg atgcttcacg tatagaagta
     1501 taaaagaagt ataaagagag gttgtgagcc tgtcgtttat tcaaaaacat actaggaaag
     1561 gagctgtgac acaaatgttg tcacagctcc tttttcagaa taaacggtgt ttaagagaca
     1621 gccaatcggc aataaggctg aacgtctctt tcacagcctc ttttttgatg agtcatcata
     1681 cattttttcg cttaaatacg agatatccta atgctaagaa caaaacgatg taagctaaat
     1741 taccgaatag catttgatta gtagaaagat gagtcaattg cttcatgatc tcttcgtttc
     1801 caatttggtt ttgcagattc aacatactga tcggattcca tttgatccac tcccattttt
     1861 ggatgagtgc aataaggata ccagatatga ttgacgaagc aaaataaaag acgattcctg
     1921 ctgagatgga tgcgccggaa ctattgataa agcaagcaat catcaacact aaacttaaaa
     1981 tcagccataa accgatataa ctacccaaag tataagtgaa caagctttgg atcagtgttt
     2041 gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa aagaatcagt ttcatcaaca
     2101 cagtgacaat aatagctaaa aggtataaat aaacagaata aataactaat gtgatccatt
     2161 tgcttactaa tatttctcct cgagaatact ttcgataaag gagatttttc aatgtaccat
     2221 attgtgcttc catcgaaatg atcgtgcttg cagctgcaat catgataaag acaatccaag
     2281 atgtggcact aaaaagctga gcagtcattt caactggatc aatgatcgtc gtgtattttt
     2341 tggctagaag tgctgttcct atgagcaaaa caactaatag aacagataaa acagcagttg
     2401 actttttctt gatcaattta aataattctt gttttacaag caatgtcatt tttcttcttc
     2461 cccctttact gaatccagaa tttccaatag agaagattcc aaatcattcg tcagatgctg
     2521 gacgtctgtc acatggatat tttgtgcaga caatagctgg atcacggttc ctaataattc
     2581 ttcgttttct tttaaaagct tgattttatc aggatcttcc aaagtatatc cagcttctgt
     2641 caagatcgtt cgagcagcta gatcatttgt tgtagagata acgatgaaat cttgcgctgt
     2701 ttggttcaat tcagccattg tacattgttt gatgatcttt ccttgattga tcaggatcag
     2761 ctcatcgacc attttttcaa gctcgctgag taagtgactg gagatcaaga aagaggtgcc
     2821 ctgagtagcc aagtcggtaa tgatttttcg cacatctctt gttgcttgtg gatccaaacc
     2881 gttcatcggc tcatctaaaa taacgataga cggatggttc agtaaggcaa gagcaattcc
     2941 caatttttgt ttcattccca acgagtagga tttcgctttt ttgtgaatgt acttttccat
     3001 cttgagctga ttcacgatag tgtcgatagc agatacatcg ttggtttcag agaataactt
     3061 cagatgatca tagcctgtta agaatggata gatacccggg tattcgatca atgctcctac
     3121 ttgttccaac cctttatgac tagagggaga aatctcattt tcaccaatac agattgttcc
     3181 atctgagtaa tggatcagtc ctaaaatagc tttcatgatc gttgtttttc cagcgccatt
     3241 aggacctact aatccaacga ttttgccagg ggataagcta aaactgacat cgctgagggc
     3301 ttgtttgtga ccaaagttct tggataattc ttggaccgaa agacttttca aaaatattca
     3361 ctccttttct atagatagta caagcataca cctgatgagt ggagaagtcg ctaggtcatt
     3421 ggacttattt cttatttttt cataaaaaaa ttgaatatta aataaaaagg cttatttttt
     3481 ctgatcagta cttattccaa gtgtctagtg gccgctagta atcttgatgc caatgatact
     3541 gatgagtagc atccccacaa agaaccaagt ggaaagattc agcttatcat gaaacaagac
     3601 cacaccaatt atgaccgaac caactgcgcc aatccctgtc cagataggat aagagagact
     3661 catcggcaaa cttttgattg cctttgacaa aaagtaaaaa ctagcaatca tgccaatcac
     3721 tgtgtaaaaa gaataattga ttttagaaaa accatcactc catttcatca tcgttgacca
     3781 gaatacttcc aaaattcctg ctatgattaa ttccatccat gccatactta cattcctcct
     3841 taactataaa aaaatacaca aaaagaggaa aatcatccct tcaaagacct aatggaatga
     3901 tcatcctctt tatcctaccc ggtggcagat tgtatatctt tttctgtagt atagcacacc
     3961 tatccagata tcggcaatct catacttttt agtaaagtgt accaagcact gacggatata
     4021 tcgttagcgg aaaagaagat gaatgctttg caatttcata gaaagaagtt gttatttatt
     4081 aatcatttaa aaaaaacagt aactagacct tatttctata tattatcggg caataaatt
//
LOCUS       DAZKMK010000149         4073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000149 DAZKMK010000000
VERSION     DAZKMK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.155"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..635)
                     /locus_tag="ACG8V1_002764"
     CDS             complement(93..635)
                     /locus_tag="ACG8V1_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0330781.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            769..1158
                     /locus_tag="ACG8V1_002765"
     CDS             769..1158
                     /locus_tag="ACG8V1_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330782.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1162..1926
                     /locus_tag="ACG8V1_002766"
     CDS             1162..1926
                     /locus_tag="ACG8V1_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0330783.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2055..3002
                     /locus_tag="ACG8V1_002767"
     CDS             2055..3002
                     /locus_tag="ACG8V1_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0330784.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3132..3872
                     /locus_tag="ACG8V1_002768"
     CDS             3132..3872
                     /locus_tag="ACG8V1_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330785.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1357 a          689 c          818 g         1209 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaatc gtaagtgaag aaaaattgaa actttataca tacaaaaaaa
      721 tgataaaatg attataatag aattataaag ataggttagg tgattaaaat gaaaacggtc
      781 aatcgtgtgt tttgtgccag ccacgtgcta agtctccttc tcagtatata cttaggtgtc
      841 tattcgattc aacagccaat tgaggtagag aacattgttt ttcagatgtc gctgacagat
      901 ggtttaattt taagcgtttt tttctttttg atactgttta ttggtaatat tttcggtagc
      961 gttgccagtt ttttgaattt ttctatttat ccactccttt cgctagcatt aggagtggtg
     1021 ggactttgtt ctctgtgctt atttcccgag ccattcagtc ctgcatttat attatttggt
     1081 acactgaacc tgttccagac aactgttgga gcatggttgc tatggcgatc aggaaattta
     1141 atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt ccatcatatt
     1201 ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt tgtcatctat
     1261 tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa aattgcgcag
     1321 cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa acagccggtt
     1381 tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt tgaatttgac
     1441 acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc agttggcggg
     1501 gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca tatagtagcg
     1561 ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat cgtaaggcgc
     1621 gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc atgggttaag
     1681 agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt cttttggcat
     1741 ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga aaaaaatcgg
     1801 cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg acatatggta
     1861 caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat aaaaaagtat
     1921 aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt ttattttggg
     1981 ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga caacatgtta
     2041 gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat gtttcaaaaa
     2101 tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg aaagagttag
     2161 ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa gcacttgtca
     2221 cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca actgagccct
     2281 attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat tattctctcc
     2341 aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc atcacaggtg
     2401 tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc ttgtttgggg
     2461 aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca gcaaaagcaa
     2521 cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc gaagtggacg
     2581 agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa gttgatcttc
     2641 ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat atgacagaaa
     2701 actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg ctagcactag
     2761 gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt ggtgtgattg
     2821 gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg atcaagcaag
     2881 atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata gaagaaaaag
     2941 gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag acaacgaatt
     3001 aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa aataatcagc
     3061 gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca tttttgagaa
     3121 ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg tacgatgatg
     3181 agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga caagaagatc
     3241 ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat ttcacgaaca
     3301 cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc
     3361 gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc cattaccaat
     3421 cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac
     3481 aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt gacacgaagg
     3541 tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc ccagacccta
     3601 gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt
     3661 atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg
     3721 tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa gaaaaaaaat
     3781 cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat gatcaaggaa
     3841 actctttggg agaaaatagc tattttaaat aagtgatata caaatttata ttcggctaca
     3901 gttggaaagg aaacgctgcg gcgtttcctt attttatact ctatccgaaa gtttatatcg
     3961 tatcctcaaa tttttttgag tttaagcgaa ttttttctcc caataagtaa gaaaataatg
     4021 gcaaaataaa aaaacaaact attttcgcag atcgtttgca aaaatagttt att
//
LOCUS       DAZKMK010000150         4052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000150 DAZKMK010000000
VERSION     DAZKMK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.156"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..1666)
                     /locus_tag="ACG8V1_002769"
     CDS             complement(161..1666)
                     /locus_tag="ACG8V1_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0330786.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1663..2349)
                     /locus_tag="ACG8V1_002770"
     CDS             complement(1663..2349)
                     /locus_tag="ACG8V1_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0330787.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2542..3963)
                     /gene="gndA"
                     /locus_tag="ACG8V1_002771"
     CDS             complement(2542..3963)
                     /gene="gndA"
                     /locus_tag="ACG8V1_002771"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0330788.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          857 c          692 g         1387 t
ORIGIN      
        1 ttttcgttca tctaaaagat acttacattt aattaacgga tgttattcta tttaattgtt
       61 cgctagtttt tttataatga catccctgaa tttctcactt gcttgtattt tgtaccttat
      121 tctttgttgg atatataaaa aaacaagtaa agcacatgct ttacttgtct tctgcctttg
      181 cgataggaat aaagattcta aaaattgtcc cttgccctac tacactttct gctaagatac
      241 gtcctttata gttttcaacc agttgtttag cgatagacaa ccccagacca tttccaccct
      301 tcgttctagc tcttgcatta tccactcgat aaaaacgatt gaagatctta ttaaggtctt
      361 ctttgggtat tccttcgcca aagtcctgaa tcgcgatctc aaattcattc agagtggaag
      421 aaatagagat atggacttct tttcgtgtag ttgaatactt tactgcgtta tccaatatga
      481 taatgattaa ttgttcaaag tgattacgat aaatttgcag ggtcacttct tttgtcaaat
      541 catcatctaa agtgatcaca tagtctggat ataatatctt gaaattatta taaacttgat
      601 aggtgacttc ttttccttta gttgtttcat tcgcataatg aacatctact tgttctgctc
      661 ttgataagtc tagcatctct tgaactaagc ttttcatacg gctgatttct tgcatgctgg
      721 cgcttaatga ttcttctaat atctcaggat catctttccc ccaacgattt agcagactaa
      781 gatgcccttc aataattgca acaggtgtcc ttaactcatg agatacgtct tccacgaatt
      841 gctcttgttg ttcaatatac aaacgcatac ggtcaagcat ctcattgaat atttcagcta
      901 aatctgccaa ttcatcattt gtgtcgattt caggcatgtg gatatctgat tgcggatctt
      961 tacgtatagt atccattgta tctctgagta ccttgagtgg ttttaagaaa taagaagata
     1021 aaaagaaacc taaaatacta cttaagatca aagaaacaac ttctaaaaca ataagagtca
     1081 ataatagctt atttcgaata tcatagaaag aagataattc atagaagctt tgcgcatagc
     1141 cgatcttttc tcttgtacct ttagaatata taggctggat agataaaaag ccagtttttc
     1201 cttctagtgt aacgatcgtt ggaactttac gtgtagtctg aatcaggttt cgactgtttt
     1261 catgggtttt aaaaatcaag ttctcgtcca aatcataaac ataaagatcc attgatggct
     1321 gccctagctc agctataaag gaatcgatct tcattaagtt tccttcgacc agtgaagtat
     1381 gatctacgac ctcatctccg tcatttgtcg ctttcgtcag attacgatac gtatttacta
     1441 atgtcagttc ttcatccgag ttagccaaac gagaagctac ttctgaaatc gtacgctcta
     1501 cattgtttct ttcttttgcc acaatcagat tgatagatga tttataggta atgacagcga
     1561 aaattgtaaa cacaacgaat atgaagaaag aactcgcaaa ggcccacttg attgtcaagg
     1621 agggcccttt cagttctctt ttttccgatt ttttcggctt tatcacgagc gcatcacata
     1681 acctgttccg cgtacagttt ggatatagct ttcctccccg ggaacgtcaa ttttgttgcg
     1741 aagataacga atatatacgt ccactacgtt tgtttcaact tctgtttcgt atccccacac
     1801 tttatttagt aaaacgtcac gtgctaatac cacgttcacg ttttccatta aagtaagcag
     1861 caactcgtat tctctttttg ttagttcaat tacttcattt ccgcgacgta cgacacggtt
     1921 ttctttttcg atagtcaaat cgcgataagt aatagtagtt tgtttagcca catttttatc
     1981 gccttcaata tcgattcgac gcaacaatgc acgaagacgt gctaataatt cttcgatagc
     2041 aaatggtttg acgatgtagt catctgctcc gtggtcaagt ccagatacac gatcgatcac
     2101 agaatctctt gcagtcatca taatgattgg tgtatttttg acttggcgta cacgtctgca
     2161 gacttctaaa ccgttcaatt ctgggagcat cagatccaac aatatggcat cccactcatt
     2221 atttaatgcc gcatctaaac cagttcggcc gttgtagtgg acttctgcgt tatacccttc
     2281 atgcttcaac tctagctcga caaatcgtgc caagttcttt tcatcttcaa tgattagaat
     2341 attgctcatt tattatttta tcctttctat tctactggct ttttaaaaac tctatcatta
     2401 taccgataat atgcttaaaa atctatagtc attttatcat ggatagcaat tttaaaaaag
     2461 agctgaagtc tactcataac aatcgacttc agctctttcg tcctcttttt attttctcat
     2521 ttcctaacag ccgaaagact attattcttc atggtaccat gagtaatgga agattccttc
     2581 tttgtcgaca cgctcatagg tatgtgcgcc aaagtaatcg cgttgggctt ggattatatt
     2641 tgcaggcaga cgttctgctc tgtatgaatc gaagtaagcg atggctgatg agaaagtcgg
     2701 tacaggtacg ccagcttgga cagccaaggc aacgacgtct ctgactgatt gctgatattt
     2761 tttcgtgatt tcaacgaagt aatcatctag caataaattt tcgatctcag gatttttgtc
     2821 ataggcatcc gtaatttttt gcaagaatcg agctcggatg atacatccag cgcgccagat
     2881 ttttgcgatt tcaccaaatg gcaagtccca atcaaattct tttgaagcga cacgtaattg
     2941 agcaaatcct tgggcataac tcatgatttt actgaaatac aacgcttctc ggattttttc
     3001 gaccaattct tttttgtctc ccgtgaactc aaaatcgttc gtacgagaca aaatcttgct
     3061 tgcttgtacg cgttcttctt tataagcaga aatatagcgg gcaaatacag attcagtgat
     3121 caatggaagt ggcacaccta agtcaagcgc gctttggctt gtccatttac ctgttccttt
     3181 attcccagct gcatcaagga tcacatctac tactggttta cctgttcctt catcgtcttt
     3241 acgtgtcaga atgtcagcag tgatttctat caaatagctg tctaattcgc cttgattcca
     3301 ttctttaaag atatcagcca tttcttctac tgaaaggttt aaaatattct tcattaagtc
     3361 ataagattca gcaatcagtt gcatatctcc atactcaata ccgttgtgta ccattttcac
     3421 ataatgtccg gctccatttg ggccaatata tgtgacacat ggtgctccgt cttctgcttt
     3481 tgcagaaatt tgctctaaga ttggtgcaac tagcgcatag gcttcttttt gtccaccagg
     3541 catgattgaa ggtcctttta aagctccttc ttcaccaccg gaaacgcctg taccgataaa
     3601 gttgatgcca gagttggcta actcttcatt acgacgcatc gtatctttaa agaatgtatt
     3661 tcctccgtca atcaaaatat cgcctttatc aaggtgagga agtaactctt ggattgttgc
     3721 atctgttgcc ggtcctgctt tgaccattag caagatccga cgtggtttct cgatagattc
     3781 tacaaattct tcgattgtat atgttgcttt gaaattttta tctgggtgtt cttctactac
     3841 agcggttgtt ttagcccctg tacgattgaa taaagccact gaatagccgc ggctttctat
     3901 gttcaaggct agatttttac ccataacggc cataccgaca acgccgattt gttgttttga
     3961 catatgaact cctcctacat acgggatttc tccctaatct cttacattat agcttaacac
     4021 ggctacattg caaactaaag aacaacatat tt
//
LOCUS       DAZKMK010000151         4033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000151 DAZKMK010000000
VERSION     DAZKMK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.157"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..639
                     /locus_tag="ACG8V1_002772"
     CDS             <1..639
                     /locus_tag="ACG8V1_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0330789.1"
                     /translation="YVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKL
                     AQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLS
                     PLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTA
                     KIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            656..1090
                     /locus_tag="ACG8V1_002773"
     CDS             656..1090
                     /locus_tag="ACG8V1_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330790.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1707..>4033
                     /gene="esp"
                     /locus_tag="ACG8V1_002774"
     CDS             1707..>4033
                     /gene="esp"
                     /locus_tag="ACG8V1_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0330791.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1475 a          562 c          770 g         1226 t
ORIGIN      
        1 tatgttaaaa atggagatat tcgaaattta gaaaacatgg tttttaatct gagtaatgga
       61 ataattccaa gtgttagtgg agatacgatt cggtctgaaa aaaattattc tatcattgtt
      121 tttgaaaagt tagcacaaac gtctataaca ttaggaatgg atataataga agcgtatcaa
      181 agtagagatg ctttgataca agaaaacgaa ttagctgtat cgttacctga ggtcttaaaa
      241 gtgagggatt ctgggattgt ttattatact aaggaaatcg gaaaaactaa gatagagcat
      301 ctttctccct taatttcttc tgtcgttcaa ttcatcggct taaatatcta taaaagaatt
      361 actgtaaagg aaatagctaa ttatttttct gtaagtgaaa ctaaattaag agaaagcttt
      421 aagaatgaaa tgcatattac gatttataat tatatctcta agcgaaaaat ttctacggct
      481 aaaataatgt tgaaatcaaa ccatactatc agtgaagttt ctttaggttt aggattttca
      541 gattcttcac acttttcgag agtattcaaa aagtacgcag gtgtatctcc taagcaatat
      601 caattaggtt tagtggacaa tttcaacaac attagctgag aggttttatt ttattatgag
      661 actatatcat ttacgtagtt tggaggagtt gaggaaattg gattttaaca aaaaattttt
      721 ttgggaaatt tctgatttga gttgtttcgt agaatctgag cgtctaagta atggtaataa
      781 tagtgtgtta aatgaatatg acaaagtagt aattaataaa gtaaaaagtt tatgtgaaga
      841 agctagagaa tctatcagtc atattaatgg tagcaatttt aaacaaacat tatttaatct
      901 gattaagcta gatgctaaaa tatcaagtta tttattcttt ttgatacatg atgaatttat
      961 ggattatcaa aaattggatc agttaattga aagagaaagc tgggaagatt attacgtggg
     1021 tctaactttt tcagaaaagt taaacaacta caagttaata gattataagt atttgagtga
     1081 aagcaattaa ttattcatgt agagtactag acactttaaa tatggacaat tcaattataa
     1141 agaaaaagaa tcaataattt aatgaggctg tgacaaatgt cattttttgt ttcgctaaaa
     1201 acgagtgaaa aaatgttgat aactccaatc attcgtttaa gattctagga atcattcaga
     1261 aatgctgtga aaccgatact ttataattat aaagaaggtg atttcaaatt ccgaagaagg
     1321 aatttgaagt caccttttta cttatgtcac agtctcattc agtaatgcgg gataaggctc
     1381 cttattccgt tatttgaata ctctgaagtt taatctaaga aagggtaata ttttgaaaac
     1441 atgcttcatc gcatagaaat gaacttaatt tcagaaagaa tacatacata tctttttata
     1501 tgttttcagc tgctgaatgg ttaaaaagca caaatatccg aaaaaaacat gaagaaatgt
     1561 actttttttg ttgtaatgtt catgaaattc tctaagtaaa ttagttaaca taaggttagt
     1621 aagattccta ttctgtgaat aatacgtatc agatttatct tttattctta ctaggaaagt
     1681 aattattaag ggagttgatt tgaataatgg ttagcaagaa taataagaga gtatttctag
     1741 aaaaaacaaa gaaacgagta ttgaagtaca gtatcaaaaa attaagtgtt ggggttgcat
     1801 ctgttttgat tggtgttggt cttgtcttgg gaacaactga actagttaaa gcacaagatg
     1861 aaatttctcc aagcagccca cttgaaacag cgataagctc cgtacaagta ggtgacaaag
     1921 tagcttcggg gaatactttt caggagaatc ctggttatac gaaaaattat aacttttcgg
     1981 atttacaatt tagtcctcaa gaactaactg gagacacact aaaaggcaat acaattggat
     2041 ttgaagttta cggaaaacat aatattgcag catcaactaa aaactgggaa atccgtcttc
     2101 aattagatga acgattagct aaatatgtag aaaaaattca ggtcgatcca aagaaaggaa
     2161 ttggatcttc tagacggacc tttgtaagaa ttaatgattc gctgggtaga cctacaaata
     2221 tttggaaggt caattatatt cgtgcaagtg atggattatt tgctggggca gaaacaaccg
     2281 atacgcaaac cgctcctaat ggggtaatta cgtttgaaaa gagcctagat gaaattttta
     2341 aagagatagg catagacaat ctcaagaccg atcgtttaat gtatcgaatc tacttggtaa
     2401 gtcatcaaga cgatgataaa attgtaccgg gtattgatag tacgggctac tttttaacag
     2461 attcagatga tttctataat agcttagatg tatctgaaaa taatcctgac cagtttaaac
     2521 atggatcagt aagtgcaaaa tatgagaaac ctaatactca aacaaaagat ggttcaggat
     2581 ccacaggtgc taatggagct ataatcttag atcataagtt aacgaaaaat tataatttct
     2641 catattcatc ttccgcaaag ggcacgcctt ggtatgctaa ttacaaaatt gatgaacgat
     2701 tagttccata tgtagcgggt atacagatgc acatggttca ggctgacaaa gtaacatatg
     2761 atgtttcgtt tgaatccgga acaaaagtag ctgatttagc aatcgagcga cgggaaggtc
     2821 atgaaaatta tggcataggt tcaataacta ataatgattt aactaaactt atcgattttg
     2881 ctaatgcaag tccacgtcca gtcgttattc gatatgtttt acaattgact aagccattag
     2941 atgaaatctt agaagatatg aaagcaacag cacaagttga agaaaataaa ccatttggtg
     3001 aagatttcat ctttgattct tggttgtcgg atacgaataa aaaattaatc cagaacactt
     3061 atggaacagg ttattattat ttgcaagata ttgatggtga tggaaaccct gacgataaag
     3121 aagagagcgg agacacgaat ccatatatcg ggaaacctga attagaagaa gtatatgatg
     3181 ttgacacaac agttaagggg aaagtattca tccacgagtt agcgggaaca ggtcacaaag
     3241 cccaacttgt tgataaagaa ggtactgtat tagcagaaaa aactatcgct ccaaatgaaa
     3301 aagatggggc tccaatttca gatactgtag aatttgaatt tacgggtgta gattcaagta
     3361 aattaatcgc gaaagatgaa ttaaaaatcc aaatcgtttc tccaggtttt gataaaccag
     3421 aagaaggttc aaccgttatt aaggaatcac caaaagcggt tgataaacaa accgtggtag
     3481 ttggatttaa accagatgct aaagaatcaa ttcggaataa taaaaactta cctgaagatg
     3541 cagagtattc atggaaaaca gagcctgata cttctaacgt tactgatagt acgaaaggta
     3601 ttgtaactgt taagatcgga aatcgaactt tcgacgtgga tgtagagttt gctgtaaaag
     3661 cttctcaagc tatggaaaat gatgcaacat acgtacctat aacaacaact ccagaaacga
     3721 cagttcaaag tggaaaacct acatttgata aaccagatgt tcctttagct aaagatgcct
     3781 tttcaatttc agatgtctat aataagtact tcggtaatgc aagtgttgat gcaaacactg
     3841 gtattgttac attcactcca gctaaaggtg taggagaatc ggagccgatt actggaacaa
     3901 ttcctattaa aattgtttac caagatggtt ctgtaggcac gaccgattta gcagtaactg
     3961 taagtaaaga tatttatgaa aatccaggag aaaacattcc tgcaggctac cacaaagtaa
     4021 ccttcaccgc agg
//
LOCUS       DAZKMK010000152         4020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000152 DAZKMK010000000
VERSION     DAZKMK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.158"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..382)
                     /locus_tag="ACG8V1_002775"
     CDS             complement(233..382)
                     /locus_tag="ACG8V1_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330792.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            844..1056
                     /locus_tag="ACG8V1_002776"
     CDS             844..1056
                     /locus_tag="ACG8V1_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330793.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1334..3133)
                     /locus_tag="ACG8V1_002777"
     CDS             complement(1334..3133)
                     /locus_tag="ACG8V1_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0330794.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3257..3853)
                     /locus_tag="ACG8V1_002778"
     CDS             complement(3257..3853)
                     /locus_tag="ACG8V1_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0330795.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1444 a          711 c          689 g         1176 t
ORIGIN      
        1 attatacaga taataacgga atattatcat cgaaacagct aacaaaattg tcataaaaaa
       61 cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa gagagacaat
      121 ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt tacaaacgtc
      181 ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact ccttatctag
      241 attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg ttattttcgt
      301 tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg cggtattgca
      361 tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt agttcttgaa
      421 agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt ttgatgcaat
      481 taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc tattttcttt
      541 taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa tataaatcat
      601 atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg aacaacatag
      661 gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat agttatctaa
      721 aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt ttcaaataca
      781 aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa aagagggaat
      841 gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg agtatctcat
      901 gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta tgttgaaagt
      961 tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct cgtaaaattc
     1021 tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag aattcgcaag
     1081 aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca aaaagaaaaa
     1141 gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct attttagcta
     1201 ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc gtcaaacaaa
     1261 aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga cttttttcat
     1321 taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct gaataagtga
     1381 tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg gttatgatac
     1441 catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt gtccatacat
     1501 atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc gtactatatt
     1561 ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa ggtaagatgt
     1621 agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg tacgtcaaac
     1681 tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca aatcgttcga
     1741 caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt tggtttagct
     1801 tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact ccgttttcag
     1861 taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca tttacccctg
     1921 ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt gtttcctgaa
     1981 catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt gcttctaaag
     2041 tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact ggttttgtct
     2101 gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg aaaaaaactg
     2161 caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg gcttgtggga
     2221 taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca acaatgatat
     2281 aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt aaaagcgtca
     2341 tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc atactttgtg
     2401 ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg ccaatcacaa
     2461 taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt tcccgaatgg
     2521 ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc agtcgaatcc
     2581 caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc caacgattag
     2641 caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga tcagaattaa
     2701 aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac aaaaaagtaa
     2761 tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc aaagagattg
     2821 gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg aaataaacta
     2881 acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga tgttgagaaa
     2941 aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt aaaataaaac
     3001 gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc atcagcaaga
     3061 catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct aagctacgtt
     3121 tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa tgaaaaagga
     3181 aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat caatgactaa
     3241 ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca taaatcaagg
     3301 ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt gtatatcgtt
     3361 tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga gcaactggaa
     3421 gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc cagccaatat
     3481 atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca taacgacgcc
     3541 ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag ataaatccag
     3601 cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga atgattggaa
     3661 taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg atggaatcac
     3721 ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata aaataaatcg
     3781 tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt tttcgagagg
     3841 cagcaatatt cattattcat caactcctta gaggttcttt gttaactata ctttactaga
     3901 aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt gtaagttcat
     3961 cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt taataacttg
//
LOCUS       DAZKMK010000153         4013 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000153 DAZKMK010000000
VERSION     DAZKMK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.159"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            764..1804
                     /locus_tag="ACG8V1_002779"
     CDS             764..1804
                     /locus_tag="ACG8V1_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0330796.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2628..2960
                     /gene="traE"
                     /locus_tag="ACG8V1_002780"
     CDS             2628..2960
                     /gene="traE"
                     /locus_tag="ACG8V1_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0330797.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3579..3920
                     /locus_tag="ACG8V1_002781"
     CDS             3579..3920
                     /locus_tag="ACG8V1_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0330798.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1468 a          510 c          721 g         1314 t
ORIGIN      
        1 atttaagatt aattttttta tgacttgtac aaatttatct gatattctct tattaagaac
       61 tacctaattt gggtagttct tattttttac attttaagta gtatttaatt gcaatttggt
      121 agtatttttt tattatctta ttcttagaac ggtagtactt aatataaata taggtagtat
      181 ctaatttaag tattaatggt agtatctaaa ttaacaggta gtatgtaact actttaaagt
      241 aatttttttc agattaacac agtagtatct tattaaataa gttaatttta atggtagtac
      301 ataattatta tatatatctg acattggtag tacttattgt attttttagt agtatataat
      361 ctattgtttt ctagcctaaa gccttgtctt ataagaataa tataatatat actactgaaa
      421 taaggtagta tatattacta gtaatggtag tatgttttat atgcataagg tagttcttaa
      481 tttatttttt gttttgacac tggtagtact taattatctt taattttaaa actaaatttt
      541 attcatacgt ttttctggta gtatttaatt ataaataata tttatttaaa gtagttctta
      601 atgactttaa aggtagtaca taattcattt tatttctgat tttaggtagt atttaattaa
      661 aaaacagttt taatgcgttt gcatttttta tttatctttg ctattttcga aaagagtagg
      721 taattttaca taaataaaac aacgtgaaag tagaggatct acaatgactg attttaaatt
      781 ttttaaggcg gatagagttt acaacgaatt attttatcaa tttccaaaag tctttattgt
      841 ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt gcctatatgc ttttaaaagc
      901 aagattagag atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg tttattttac
      961 ttatacgaca aatgaactat gtagagtatt aaactgccaa aaacaaaaag cgatagcaat
     1021 caaaaaagag ttggaatcct ttggtttatt attacaaaag cagatgggat ttaacaaaca
     1081 gttagggaaa aataatccta atagactata tctagcagaa ttaaaagtct cagaaaatga
     1141 tatctactta ctcgaaaaat ttgatagaga gaatagggaa aacgttgata aatcagaggg
     1201 tatgaaaatc atacccaccc tcgacgaaaa atcagacgct gaatcccttg gggctcaaga
     1261 gggtatgaaa atcataccgt gccaaaacgt tgataaatca gagggtatga aaatcatacc
     1321 agaacttaat aataatatat tagacactaa tagacacaat atagacactg aaaaagaccg
     1381 cctacaagat caattgttgt tagacaattt tgagacaatt atgacaaacg acagcattgc
     1441 tacgtttgtc cctgaacgag tattaaattt gataaaaaca ttttcttcaa gttacagtga
     1501 agctcaaaaa accgtccaga ctattcataa tgcaaagaaa aaagctgaaa tagaaagtga
     1561 tatttcgata gtttttgaag aactcgatag ttattatgtc aatgcagaac aagaattata
     1621 cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa atatccagaa
     1681 cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg ctaaagtatc
     1741 aagtgaaaaa cgtttgaatt atgcaagctc cccaagcact attacgaaag actggttaga
     1801 gtgaaacttt actgacaaag tgattgttgc ttcatggagt aacattaaaa ttaaaaattt
     1861 tttattattc taattatatt acgaatatta caaaataata gctattttaa tagaatttat
     1921 aagttaaaat attatgataa aaatcacaaa cgtagccttt tacgtcataa gtgttaaaag
     1981 aaataattaa tttcaaatat agaaaaggga acgtatgttt gcatactcct tttttttgag
     2041 ttataataat acgcgaagag agataatttc attatttaaa caagctaaaa atcttgatat
     2101 tacttctttt caggcaatac gaatattatt cgcttacatt ttttgtataa atttaattgt
     2161 ctgctatgct tgtgatgaat tgattttcgt tttgcacttt tggatgataa atttggacaa
     2221 ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac cttcgtcagc
     2281 gttcatacac ggcattaaga tttggccctt aatgcgttaa cctttcagtg ttacggtatt
     2341 acccgcaatg gatttagtat aacagatatt caaaagtgat accacttttt ttggctgtgt
     2401 taaggttaaa aatacgtgat attacgatga atctatgcct aagcataatg ttataaaaat
     2461 gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta gagggaacgc atgtttggat
     2521 gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt ctctattttt gaaaaataac
     2581 aagaaaaact tgttaaaaat atttaaagaa attgggtggt ttattttatg tcatatacag
     2641 gaattttgag tctcaaagat atttgtcact acggtaaacg gtgtaccgct acagagaaaa
     2701 tcacgaagaa actatcaact ggccaaagta aaaccgttgt gcagtgtaag aaatatataa
     2761 tccagaaaga caaagtctca gaggaaatga tttattacat tgggaaacaa aagcaaataa
     2821 ttttaaaaga tcctatcccc ttaaaagagc tatatccaac aatcaaacat gtttatgacc
     2881 aaaacggtgt actgattggt cgaagaaaaa atggagtctt gcgttgtacc gcaaaaggga
     2941 tggggcgttt gatcggctga attgatcatt ctcataactt gtattttatc gtttttcgct
     3001 tgcttgaaaa tattgtcgag acttctaaag gaacttagag tcactagaaa aataaggagt
     3061 agacttgatc tatcaacaat tcccttcggg gagtgacggg gtaggtttta acgattggta
     3121 acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga aatgaatttg
     3181 tgtaaataat aagcgcagag taaacaagag ggaatttaga agttagacaa gttagaatag
     3241 cgtctagatt aatcatgagc agctactaag ggatctagac tttgacgcaa actcatggaa
     3301 taaggagtag gtcttctcgt aatggggagg acgcacaaga aattgtgtcg tgtgctgcag
     3361 tacatgtaga tacttgcgag gacgttgatt attggatcaa tcgagcattt accgctgtct
     3421 actagggttt ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt atttttaagt
     3481 aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt aatataaagc
     3541 atagagaaaa tgaaatttac gtaatggagg aatgacaaat gaaaaattat gatccgaata
     3601 tacgattggg cacacatact attaaggttt ctttccaacg ttgggattac aaaggatttg
     3661 tgacgtttag aaggggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg
     3721 atttatacga tcaaacattg actgataatc caattggatt cggcttgcta cctgaagatg
     3781 atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga taatggagac gaattatctg
     3841 ttgaagatac ttggtcatat ttgagtgatt atattgtcag ctttgaaatc attgaatttg
     3901 tagcggataa agaagaataa aaactggagg aataaatttt acagataaag gcttagaagg
     3961 agtcacgacg attaataaaa aggtagtatt accttaggca ttgagtaagg aat
//
LOCUS       DAZKMK010000154         3925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000154 DAZKMK010000000
VERSION     DAZKMK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.160"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG8V1_002782"
     CDS             complement(573..1130)
                     /locus_tag="ACG8V1_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330799.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG8V1_002783"
     CDS             complement(1309..1920)
                     /locus_tag="ACG8V1_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330800.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG8V1_002784"
     CDS             complement(2033..2290)
                     /locus_tag="ACG8V1_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330801.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG8V1_002785"
     CDS             complement(2887..3165)
                     /locus_tag="ACG8V1_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330802.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG8V1_002786"
     CDS             complement(3199..3450)
                     /locus_tag="ACG8V1_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330803.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAZKMK010000155         3902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000155 DAZKMK010000000
VERSION     DAZKMK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.161"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG8V1_002787"
     CDS             <1..395
                     /locus_tag="ACG8V1_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330804.1"
                     /translation="CLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFL
                     WLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYF
                     IGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(521..1198)
                     /gene="nth"
                     /locus_tag="ACG8V1_002788"
     CDS             complement(521..1198)
                     /gene="nth"
                     /locus_tag="ACG8V1_002788"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0330805.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1205..1903)
                     /locus_tag="ACG8V1_002789"
     CDS             complement(1205..1903)
                     /locus_tag="ACG8V1_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0330806.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2171..3355
                     /locus_tag="ACG8V1_002790"
     CDS             2171..3355
                     /locus_tag="ACG8V1_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0330807.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3361..3804)
                     /locus_tag="ACG8V1_002791"
     CDS             complement(3361..3804)
                     /locus_tag="ACG8V1_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0330808.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1014 a          767 c          639 g         1482 t
ORIGIN      
        1 tttgtttatt attaaataaa tttactttaa ttgctcctat ccttgcattt attatttttt
       61 ctgtactttt aagcactact cttaaaggaa aatttatgga aagaagctct catgcaatga
      121 ttctcacttt tttatggttg ttgttcacca gaatctatat ttttctgatg agcctgaaac
      181 cgcccaaatc cattagttta tgtttagtta taaatggtat ttttttatta tcacttatca
      241 tttttattac acctttagat agatacgtca cttatttata taatcctttg gaatactgga
      301 cttattttat aggtatattg gaaggcatta ttttctacat cgggtatttt cccaataaaa
      361 ataacaactt ttatttttat cgcaataaat tgtaataagt taccatccta gctactgagt
      421 atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc cataataaca
      481 agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt
      541 ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt gatagcaaag
      601 gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg
      661 tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt tcttctactt
      721 ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg
      781 tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt gctgtctttc
      841 tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc ccatcgaaac
      901 gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga tacaaaccaa
      961 tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg
     1021 gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc gcctgggcac
     1081 ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct ccatgtgctt
     1141 cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg cttaacatat
     1201 tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct tgttgagctg
     1261 actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct tcttccactt
     1321 gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca acgtaattaa
     1381 aactataagc ctgatttaat acggcttccc gcagtgctag cttcaaaatt tccggctgat
     1441 aatggtcttc ttctagccat tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc
     1501 caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt gttttttctt
     1561 cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg gggtaaagat
     1621 tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata aatccatttg
     1681 ttacgagacg atccagtatt tgaaaaatct gatcttgttt gattcccata tccatagcaa
     1741 tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac tgtagaacaa
     1801 ataaaaattc ttcagattgt agaccgatcc tatgataatt tttaagtaaa agattagata
     1861 tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct cctccgctca
     1921 atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc tatttacttc
     1981 actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat gtttttgatg
     2041 cgtatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct tagaatgttg
     2101 acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta ttgcataaag
     2161 gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat acgtctctgt
     2221 cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt taacgctgcc
     2281 ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt ctgtttatgc
     2341 tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt ttgggcgtca
     2401 tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt ttggtttagg
     2461 aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg caggagaaat
     2521 ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa ccaaagtttt
     2581 tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta tagggggtct
     2641 attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta ctttcttaaa
     2701 tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac gttcagtaga
     2761 tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca agatttcatc
     2821 cgttattcct ttattgacag caggttttgc tgtttggttg gcagctggct ctcttcaatc
     2881 tatcttttct caattttcga ttgacgcctt tcagtggaag gctgggttgg ttggtttaag
     2941 cttctctttg attggaatct tggatactgt ctcgcaattg ttcattatgc ctcgattgct
     3001 caaaaaattt tcagaacagc agatcacaag aatagggatg ttcagtgaaa tcctcgccta
     3061 tctattcatc acattatctg gaattcttct actgccgatt ttgtttttat ttgggattat
     3121 ctgttatggt ttcggtgatt ctatttttac tcctgtattc aatggccaac tatccaattc
     3181 tgtctcagat aaccagcaag gtcttgtaat gggcggtacc caaagtatac aatctctatc
     3241 gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct gcagcaccat ttcttccgac
     3301 ggtattcgga tttttgctta tgatttttgc ctattatcac tacaataaaa aatagtagct
     3361 tcatttgcta cttcgtataa attctctgta tcgaccgata aaacgaaata ctgtgtcaaa
     3421 ttcttctttt gacatatttt caaatacagt agcatctcgc tgtacccatt tttgatggag
     3481 ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc aattgaaaat agatttcttt
     3541 tttattctct tcgctttgat aagtgtttat gatccctcta gctatcagtt tttttgttag
     3601 cttactgatg gctcctctag tcatgtaact agcattcgct aattttgtca cgttaggatc
     3661 agggattttc ccgatatgtt caatcacttc tagttcattt agagtaaatc ctgcaaattc
     3721 tttttccatt tctggtttat tacgatgaat aatctcatta gctaaatcca gatattcttc
     3781 tattagcagt tgtcttttat ccatttctac accttctttt tctttcatta tattgttttt
     3841 ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa aacccaatct gaaaccaaga
     3901 aa
//
LOCUS       DAZKMK010000156         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000156 DAZKMK010000000
VERSION     DAZKMK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.162"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG8V1_002792"
     CDS             complement(245..466)
                     /locus_tag="ACG8V1_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330809.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG8V1_002793"
     CDS             complement(564..809)
                     /locus_tag="ACG8V1_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330810.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG8V1_002794"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG8V1_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG8V1_002795"
     CDS             complement(2033..3034)
                     /locus_tag="ACG8V1_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0330811.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG8V1_002796"
     CDS             complement(3193..3459)
                     /locus_tag="ACG8V1_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0330812.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG8V1_002797"
     CDS             complement(3449..3805)
                     /locus_tag="ACG8V1_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0330813.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKMK010000157         3857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000157 DAZKMK010000000
VERSION     DAZKMK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.163"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACG8V1_002798"
     CDS             172..516
                     /locus_tag="ACG8V1_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0330814.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACG8V1_002799"
                     /pseudo
     CDS             517..936
                     /locus_tag="ACG8V1_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="ACG8V1_002800"
     CDS             complement(1028..1378)
                     /locus_tag="ACG8V1_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330815.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ACG8V1_002801"
     CDS             complement(1344..2675)
                     /locus_tag="ACG8V1_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0330816.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ACG8V1_002802"
     CDS             2931..3497
                     /locus_tag="ACG8V1_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0330817.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ACG8V1_002803"
     tRNA            3642..3729
                     /locus_tag="ACG8V1_002803"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
BASE COUNT         1420 a          661 c          747 g         1029 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacac
//
LOCUS       DAZKMK010000158         3664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000158 DAZKMK010000000
VERSION     DAZKMK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.164"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACG8V1_002804"
     CDS             <1..277
                     /locus_tag="ACG8V1_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0330818.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="ACG8V1_002805"
     CDS             420..641
                     /locus_tag="ACG8V1_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330819.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="ACG8V1_002806"
     CDS             657..1499
                     /locus_tag="ACG8V1_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330820.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="ACG8V1_002807"
     CDS             1562..3364
                     /locus_tag="ACG8V1_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0330821.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="ACG8V1_002808"
     CDS             complement(3423..>3664)
                     /locus_tag="ACG8V1_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0330822.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DAZKMK010000159         3602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000159 DAZKMK010000000
VERSION     DAZKMK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.165"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8V1_002809"
     CDS             <1..186
                     /locus_tag="ACG8V1_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0330823.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG8V1_002810"
     CDS             329..553
                     /locus_tag="ACG8V1_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330824.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG8V1_002811"
     CDS             568..1437
                     /locus_tag="ACG8V1_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0330825.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG8V1_002812"
     CDS             1418..2152
                     /locus_tag="ACG8V1_002812"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0330826.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG8V1_002813"
     CDS             2185..3093
                     /locus_tag="ACG8V1_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0330827.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG8V1_002814"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG8V1_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAZKMK010000160         3589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000160 DAZKMK010000000
VERSION     DAZKMK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.166"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..397
                     /locus_tag="ACG8V1_002815"
     CDS             161..397
                     /locus_tag="ACG8V1_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0330828.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            416..631
                     /locus_tag="ACG8V1_002816"
     CDS             416..631
                     /locus_tag="ACG8V1_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0330829.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1334..2086)
                     /locus_tag="ACG8V1_002817"
     CDS             complement(1334..2086)
                     /locus_tag="ACG8V1_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0330830.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2101..3348)
                     /locus_tag="ACG8V1_002818"
     CDS             complement(2101..3348)
                     /locus_tag="ACG8V1_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0330831.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(3424..3570)
                     /locus_tag="ACG8V1_002819"
     CDS             complement(3424..3570)
                     /locus_tag="ACG8V1_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0330832.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT         1223 a          566 c          533 g         1267 t
ORIGIN      
        1 tgaaaacagt tcttgaacac taactttttc attcctgaat caaatttatt ttggaaattt
       61 tactattcaa gaataaactt gataagtttc aggaagattt ttactgttaa tcaaatacgt
      121 atgttacagt agcaccgtaa aaaatattat ttaaagaagc gtgtttaaac atgcaaaatg
      181 taaaggaact aagtattgtg gaaatgcaca aactgttggt ggaggcaata atgcttgggg
      241 aaaattgggg caagttgtag gtggacttac cacaggggca gtaggtggtg caggtttagg
      301 aacagcaatt tgcggaccag cttgcggtgt tgttggaggg ttatatggag ctgtcgctgg
      361 aggtgctgca gctggctggg atgctcgaaa aaaataaaaa aggagaataa gtagtatgca
      421 tattaaaaac acaaaaacaa catttatatt atcttcagaa gagttgaaaa atattcaagg
      481 tgggagtgcc gtgggagttt taggcactac tttttctggg gcaacagcag gagtaaaact
      541 ttgttcagcc ggaggacctt atgcaatagc tgcttgtggt gtaggtggag ctttattagg
      601 tgctggattc agcatgtgga ccggtgcgta aacaattaaa aaatatcaaa cctcaagatt
      661 ggtttgtgtc gattacgaac ataggtatgt tctttatctt ttcaaaagtt tggaatggac
      721 agaacttttt actaaagctt cttgtgctag tttttataat tatattaaat ttttatcttt
      781 tattaaagtc agataaaaat tctttggaaa tgtaatggta gagatatttt tacaaaagga
      841 cttgcagcgc tgtagttggt gctgcaggag gacctatttc ggccgttaca ggaggtattg
      901 gtaatgtact tgtcggatgt gcattaaagc ctgttcctgc aagttaaatt tataaaaaca
      961 ggaaagtaat tatctcaaat cctatcaagt acaatcatag gattgcactt gacaggacaa
     1021 attttttaac taaaaaatga aaaacacttt ctaaaaatta gtagaatttg ccaaatcttt
     1081 cgcatcttcc accatacatt taattttaat tcccctttat ggtacttaat aaaaacaaga
     1141 aaagaactat tcatttggcc cttttttggt tccagaccat agaaatattc ctccagtaac
     1201 taatattgct gttatgcacg taactaataa cagctataaa aattggtaca actttaatag
     1261 gattcagaaa aatttgcact agtgaaggaa tctattttat tagaaaaatg caatctccct
     1321 aaagaaaaaa ccactaaaca gtgctttctt tcaacttctc cttaattttt tgtgctttcc
     1381 ctaaagtaaa gtttctggcc aactctgaat caaaatatag tgaacgtttt ttaaaatcta
     1441 tttctttaac gttaaagata tttactaaac acgctcgact aattcttgtt aacataggat
     1501 atttcttttc aagctcacta attcttccat aaaattcgta ctgcccatct ttagtacaca
     1561 atgataatct atgtggtaat tttgaagctt ctacaaaata aatgtcattt atatcaaaag
     1621 taaatgtttg agaacctatg gaaaaaacaa atgcttgatt cgtaacctgt tttgttgcat
     1681 ctatcctttc ttgcgctaat aacaataact caacaatctc gtttcgatac tcatctagtg
     1741 tttgatcttt tgttacaaat cctaacgttt caactcttct ttttattgtt acgggtaaca
     1801 tctcatcatg agtagttgta aaaatgattt ttgcttgaac atcatatttt ctgattactt
     1861 ctgctaattc tataccgtta acttcatgat ttaaatcaat atctaagaaa tatattcctt
     1921 gctttggtcg gaattgtttg aggtattttt taacttctaa cggactttgt gttttcaata
     1981 ctatcttaaa tactttatcg tgaaaaagaa tgaaattatc aataattcgt tctaactgat
     2041 ttaattgtat aatttgatct tcacataata ttattggata actcataaat cctccttccc
     2101 tcaaaacaac aaaataactt gagtagtaaa ttgatacgga tcgttttgat agttcacaaa
     2161 catattggga ttctttttat tgatttcctc tatactagat aaacctaacc cttgatgtcc
     2221 cttttttgtt gttattcctt ttttcattag agtatctata gagagtagta tctctctaca
     2281 actattcgat atcgaaattt ctatctgttt atcatctcgg taaatagcta gagacagttc
     2341 tctatcttga ctctctatag atgcttcaat tgcattatcg agaataattc caagcaccct
     2401 aatacagtca aatattggaa caggaacttc aataatagta cttggacatt cgaatttaca
     2461 acaaatctta ttgttatttg cttggtatag cttcgctatc aacaaacttt tcaaatattc
     2521 attttttata ttttttaaat aacgatattc aaacttattg gttagataag aattagaata
     2581 gatctctaat tcttttattt ctttttccaa tatcgaatca tggttttgat tagcgatttc
     2641 ttttaaactc agcaataaat ttttatagtc atgtctaaat ctggcaaatt tttcttgatc
     2701 tttttccagt gaatcagtat atttttttag ataaaccaat tcttttcttt tcaattgctg
     2761 ttcatattgt tctcgttgcc taatagttat tctaataaat aaaaatagaa caacaaacat
     2821 ttgaatgatt aaaaatatta agacaccaaa cacaaaatga tcaaatactt gatattgatg
     2881 agcaacataa gaaacaaata aaccaattgt aagtaaataa atcatgaata tagcagtaag
     2941 tttagaactg gactgttcaa caatcctatt tatagctaat tttctatata accaaataaa
     3001 catcagcaaa aacaaagaat caagcattat ctggaatact acataaccat acccttttat
     3061 cccttcaaat gaaaacatat acgtcataac catggaagta ataatagatc tgaaatcatc
     3121 aataagcata cataatagta atttatttaa aagcactata ttagttttct tcttttcctt
     3181 taatataaaa taacttccga ttataggtat agctgcggaa acatcagtaa ataattcaac
     3241 aaaaaaagtg agtggtatca ataaacatac aaaaagatat tttcttttgt taagatcagt
     3301 attagtcata taaaataaga gaaaaaagaa aaagtttccc acgaacattc ttaatagata
     3361 agcttttgta tcgatagtaa tcaagagatc ccctcctgat tttaggatat cataaaaaaa
     3421 attttagtta cactttttaa ataatgaaaa aacacattcc actaaatttg atgccggttt
     3481 tccttgaggt tttgttccag cacctccttt aattttttta agctcttgat ctgaacattg
     3541 tttagcattt agtctatttt tttcttccat tacgattccc cctacaaac
//
LOCUS       DAZKMK010000161         3575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000161 DAZKMK010000000
VERSION     DAZKMK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.167"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1194
                     /gene="pgk"
                     /locus_tag="ACG8V1_002820"
     CDS             4..1194
                     /gene="pgk"
                     /locus_tag="ACG8V1_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0330833.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            1298..2053
                     /gene="tpiA"
                     /locus_tag="ACG8V1_002821"
     CDS             1298..2053
                     /gene="tpiA"
                     /locus_tag="ACG8V1_002821"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0330834.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            2169..3467
                     /gene="eno"
                     /locus_tag="ACG8V1_002822"
     CDS             2169..3467
                     /gene="eno"
                     /locus_tag="ACG8V1_002822"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0330835.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         1245 a          674 c          752 g          904 t
ORIGIN      
        1 aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt
       61 gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca
      121 gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac
      181 cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa
      241 cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa
      301 ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga
      361 tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg
      421 gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct
      481 tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa
      541 gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt
      601 ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat
      661 aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc
      721 ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca
      781 ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt
      841 gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga catcggccca
      901 gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga
      961 cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca
     1021 atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc tgcagcagca
     1081 gaacaattag gatttgctga caaattcaca cacatctcaa caggtggtgg cgcaagcttg
     1141 gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc
     1201 aagtttgctc aattctgacg agtaatcgat caaaaggtga agggagtttt catgactccc
     1261 tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg
     1321 taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa
     1381 caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca
     1441 agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg
     1501 ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt
     1561 tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga
     1621 catcaataaa aaagcaaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg
     1681 cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac
     1741 agctggttta gctgatcttt cagctgaaca agtgtcaaac atggttattg cttatgaacc
     1801 aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg
     1861 agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat
     1921 ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt
     1981 tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcatttttgt cattgttaga
     2041 cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc
     2101 aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga
     2161 gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta
     2221 acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc
     2281 cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc
     2341 gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag
     2401 caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag
     2461 acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg
     2521 ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta ggcggattca
     2581 acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata
     2641 actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag
     2701 ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat
     2761 tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag
     2821 gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg
     2881 ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac
     2941 tagctgactc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat
     3001 tagtttctaa atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg
     3061 gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt
     3121 tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc
     3181 ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta
     3241 aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa
     3301 tctctgatat cgctgttgca acaaatgctg gccaaatcaa aactggttct ctatcacgta
     3361 ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg
     3421 cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg
     3481 aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa
     3541 ctctctactt gctgaaatat cagcaggtag agagt
//
LOCUS       DAZKMK010000162         3541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000162 DAZKMK010000000
VERSION     DAZKMK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.168"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..971)
                     /locus_tag="ACG8V1_002823"
     CDS             complement(267..971)
                     /locus_tag="ACG8V1_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330836.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1158..1403)
                     /locus_tag="ACG8V1_002824"
     CDS             complement(1158..1403)
                     /locus_tag="ACG8V1_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330837.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1420..2271)
                     /locus_tag="ACG8V1_002825"
     CDS             complement(1420..2271)
                     /locus_tag="ACG8V1_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0330838.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2437..3441
                     /locus_tag="ACG8V1_002826"
     CDS             2437..3441
                     /locus_tag="ACG8V1_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0330839.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1098 a          604 c          687 g         1152 t
ORIGIN      
        1 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
       61 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      121 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      181 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      241 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      301 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      361 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      421 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      481 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      541 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      601 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      661 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      721 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      781 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      841 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
      901 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
      961 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1021 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1081 cgtattttcc cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat
     1141 aatccgttcc attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa
     1201 taaatgactt catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc
     1261 agttcttcga tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct
     1321 gcatcgatca aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa
     1381 ttcaagcgtt cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg
     1441 gtcttttgct ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt
     1501 taattcaaaa tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg
     1561 aagaggaagg aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc
     1621 atgtgcttca gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg
     1681 actatatgct tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa
     1741 ttcgcctggt gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag
     1801 tgtatctaga tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc
     1861 atacagttct tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa
     1921 agcaacaggg tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt
     1981 caatgattct tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt
     2041 tgttgtcaag aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct
     2101 ttcttcattc ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc
     2161 tttaacagag gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac
     2221 cgggatttcg taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc
     2281 tcctttttta ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt
     2341 gtttctactc ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt
     2401 ttttttccgt aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca
     2461 attctgcata ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat
     2521 ctgacaaaag aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct
     2581 caaacaaaaa ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct
     2641 caagaaaatt ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa
     2701 gaaagtgaag aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg
     2761 atgtacttat ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca
     2821 gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca
     2881 caaaaagcag atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa
     2941 aaaaaatatg agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt
     3001 gttcctgcac cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt
     3061 ggaaaaaaat ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat
     3121 gatacgatag aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag
     3181 gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca
     3241 gaagcacctc ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa
     3301 ttcaagctat caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat
     3361 ttgattgaat ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat
     3421 gaagtattga atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca
     3481 gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt
     3541 g
//
LOCUS       DAZKMK010000163         3487 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000163 DAZKMK010000000
VERSION     DAZKMK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.169"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8V1_002827"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8V1_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0330840.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8V1_002828"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8V1_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          994 a          764 c          541 g         1188 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaaact aactatccat caataacgtg ttttattttt taaaatttct
     3481 ataaaaa
//
LOCUS       DAZKMK010000164         3449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000164 DAZKMK010000000
VERSION     DAZKMK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.170"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8V1_002829"
     CDS             complement(150..1337)
                     /locus_tag="ACG8V1_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0330841.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1495..1824
                     /locus_tag="ACG8V1_002830"
                     /pseudo
     CDS             1495..1824
                     /locus_tag="ACG8V1_002830"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /locus_tag="ACG8V1_002831"
     CDS             1872..3272
                     /locus_tag="ACG8V1_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0330842.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          978 a          652 c          684 g         1135 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgctttta
//
LOCUS       DAZKMK010000165         3200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000165 DAZKMK010000000
VERSION     DAZKMK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.171"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8V1_002832"
     CDS             301..585
                     /locus_tag="ACG8V1_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330843.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8V1_002833"
     CDS             588..1004
                     /locus_tag="ACG8V1_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330844.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8V1_002834"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8V1_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0330845.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG8V1_002835"
     CDS             2522..2608
                     /locus_tag="ACG8V1_002835"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0330846.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACG8V1_002836"
     CDS             complement(2911..>3200)
                     /locus_tag="ACG8V1_002836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330847.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAZKMK010000166         3085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000166 DAZKMK010000000
VERSION     DAZKMK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.173"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1294
                     /locus_tag="ACG8V1_002837"
     CDS             <1..1294
                     /locus_tag="ACG8V1_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0330848.1"
                     /translation="TIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEP
                     TVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPD
                     GTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWK
                     EKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEP
                     DASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKK
                     APNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGT
                     KVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSK
                     VFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1428..2771
                     /locus_tag="ACG8V1_002838"
     CDS             1428..2771
                     /locus_tag="ACG8V1_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0330849.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2883..>3085
                     /locus_tag="ACG8V1_002839"
     CDS             2883..>3085
                     /locus_tag="ACG8V1_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0330850.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTT"
BASE COUNT         1226 a          421 c          582 g          856 t
ORIGIN      
        1 tacaattgat accaacacac caggtaatta cgaaggtgtc attgaagtaa cgtatccaga
       61 cggtacgaaa gatacggtga aagttccagt agaagtgaca gacaatcgtt ctgacgccga
      121 taaatatgaa ccaacagtag aaggcgaaaa agtagaaatt ggaggcacag tagatttaac
      181 agataatgtt actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc
      241 cggcggtaca attgatacca acacaccagg caattatgaa ggtgtcattg aagtaacgta
      301 tccagacggt acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctaa
      361 tgcagataaa tacaatcctg aaggacaaaa agtgactaca gacttaaata aagagccaga
      421 tgcatctgag ggaattaaga acaaagaaga tttaccaaaa ggaactactt atgcttggaa
      481 agagaaagta gatgttagca cagctggaaa taaaaaaggt actattgtag tgacatatcc
      541 agatggatca tctgatgaag ttgaagtaga cgttacagtg acagatactc gttccgatgc
      601 agataaatat aatcctgaag gacaaaaagt gactacagac ttaaataaag agccagatgc
      661 atctgaggga attaagaaca aagaagatct accaaaagga actacttata cttggaaaga
      721 aaaagtagat gttagtacag ctggaaacaa aaaaggtact gtagtagtaa catatccaga
      781 tggatctaaa gaagaagtag aagttactat ttctgtagta gataaaaaag caccgaataa
      841 acctcaagtt gatcctatta cagagggtga ccaaattgtt actggtaaaa ctgaaccaaa
      901 tgcagaggtg acagttacat taccagacgg aagtcaacac catgggacag ctgataaaaa
      961 tggtaatttt acagttaaag ttcctaaatt agaggcaggg acaaaagtga tagtaactgc
     1021 aactgatgaa tctggtaata ctagtgaacc tactaatgta gttgtttctt ccaacaaaaa
     1081 agacagtgga aaaaacggta gtaaaggtag taagactgac aatcaaggta gcaacagcaa
     1141 tcaagacaaa aatagaggca aaagccagtc ctcaaaagtt tttcctaaga cgggagagag
     1201 tgacagtaat atctttacta ttagcggtgg tttaatactg ttaggaacgt taggattatt
     1261 agggtataaa aatcgaaaaa aagagaatga atagaaaatc aatttcttaa gtcttctctg
     1321 agaagaggaa gaatcttttt cttcctcttt ttgctttttt atgatgggaa ataagtgtta
     1381 gttaaagatg aatatacttt ttatagaaag gaaaagaatg taattaagtg gaaaaaatag
     1441 taattattgg ttcaaaccat tcagggctag ccgcagctcg atttctaaac ttaagcgatt
     1501 caaattatga agttattcta atcgataaaa aatcagaaat tagttacttg gaatgtggga
     1561 catcgttatt tataaaagga gaagtaagta atttagaaga tcttttttat caaaatagaa
     1621 tgacactaaa ttctgagata aaaaagttat ttctagagac agaagttgac aaaattgata
     1681 ttcaaaataa aaagattagt tgtgtaaaaa aagatggtac aagattctct caagaatatg
     1741 acaaagttat attagctaca ggttcaaagc aaattatgct agatataccg gggaataatt
     1801 tagctaatat tttttcgata aagaattttc caaatgcaaa agagattttt aaagctttgg
     1861 atgatagtac tattaaaaaa gtaggaatta ttggagcagg atatattgga gttgaaattg
     1921 ctgaagccat aagaaaaaga gggaaagaag tatatctttt cgatgttgca gatagagtgt
     1981 tgtcgactta ttacgaccgt agtttttcag ataaagttga agaaatacta tcaaaaaatg
     2041 gaatacatct ttgtctatca gaaagtccta aaaaatattt aggaagaaaa aagattgaaa
     2101 aaatagtcac tacaagtggt acccaatact ctatggatat ggtcgtacaa gctataggat
     2161 ttctatctaa tagtccaatt ggggaaaagg aactcttgag agatgttagt ggagcatatc
     2221 taactaatga ataccagcaa acaaatataa aagatatata tgctattggg gattgtgcta
     2281 caactttttt tacgtccatt aatagaagta gtgttgattt ttctatttct aatgctctaa
     2341 gaacagctta tattgcaact caacatatta acaatcaaga ttttacacct tctggttccc
     2401 aatttacaaa tggcttttct atatttaatt acaattttta cgcagcaggt ttgaatttaa
     2461 aaacagcacg attattaagt gagtctatag attatataga aatagaagat ttcattagac
     2521 ctgctttttt gagaaaaaat agtcgtgtaa aattaagaat tagctttgaa aaaaatacaa
     2581 agagaatagt aggtgtacaa ctttgtagtc aagaagattt atctggacta ttatcaatgt
     2641 tttctcttgc tatagaagaa caaattacat tagacaaatt gaaaatgtta gattatttat
     2701 ttcatcctag ttttagccaa ccctataatt ttattttaaa ggcagtaaca ggattaaaca
     2761 ttgaaacata atcgttacta aatgaaatca agaaatatac tgtattactc aattcattga
     2821 aaatatttat cataattttt aatagaaaac actattaaag agaaaaaaga gaggtaaatc
     2881 aaatgaaaaa atctttaaaa cttgcatcag cagttatatt aatgtcccaa ctccttatag
     2941 caacaccttt ccatgtgttg gcaagcgaaa attcaacata caaaattgca aaagaaagaa
     3001 ccgagacgac tgaaatgagt aattccgatg taacagcaga gagtagtgaa cttttgacaa
     3061 cttcatcaag tgaaagtagt acgac
//
LOCUS       DAZKMK010000167         3072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000167 DAZKMK010000000
VERSION     DAZKMK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.174"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8V1_002840"
     CDS             complement(37..1257)
                     /locus_tag="ACG8V1_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0330851.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8V1_002841"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8V1_002841"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0330852.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8V1_002842"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8V1_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0330853.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          855 a          629 c          526 g         1062 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc tt
//
LOCUS       DAZKMK010000168         3061 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000168 DAZKMK010000000
VERSION     DAZKMK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.175"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            277..1092
                     /locus_tag="ACG8V1_002843"
     CDS             277..1092
                     /locus_tag="ACG8V1_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330854.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1218..1475
                     /locus_tag="ACG8V1_002844"
     CDS             1218..1475
                     /locus_tag="ACG8V1_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330855.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1727..2299)
                     /locus_tag="ACG8V1_002845"
     CDS             complement(1727..2299)
                     /locus_tag="ACG8V1_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0330856.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2315..2920)
                     /gene="fic"
                     /locus_tag="ACG8V1_002846"
     CDS             complement(2315..2920)
                     /gene="fic"
                     /locus_tag="ACG8V1_002846"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0330857.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1010 a          539 c          472 g         1040 t
ORIGIN      
        1 ttcaaaaaag aaaaaacttt ccttaaaaac aaaaaatagg agtattcagt ttcttaatag
       61 ttttgtaata gttgtatgta gaagttttct taatagtttt tgtaataatt tattaatcgt
      121 agtttatata acttatttgt aagtgactag agaattaaat aaaaaataat tactatctag
      181 tacgaaatta attacatagg ataatatatt aaggtgctgg actacaagta aaaaatactg
      241 tatgatcatg acagattcaa gaaaggagca ataagattgg ttaagatagc aggactttct
      301 aaaggtggaa aaaccgtttc acaactttat aaacaaaact acagagactg gagacagttc
      361 agaaaaaaca ttaaggattc ttactttatt ctaccaacag aacttgaaaa atatttaccg
      421 aataacaaag ctattaattt atatctgtat tactgtttta aggcaaagaa tgaaggcggg
      481 gattcctggc attcggtcaa taccatagca gagtctctag gagtgacaac taaaactata
      541 aacacctgga ataaaacctt agttgattta ggtatgattg cacgtataga cgataaccat
      601 ctcagtaaat cgacaatttt acttcctctc tctagttact ataaattagc ccttgatagt
      661 tctttaaagg atgttaccga gagcactgtc catgaaattg atggcaactt ggttgcagtt
      721 tatcatttgt ttgaatggag aaaaaatgag gctgatgaaa attataacgt tccttataac
      781 acactctgtt gtgtttttaa aagaaaaaca caaaaagata ctatctataa atttatcttg
      841 gtccaagatg agaaaataag taatttcgaa ttaagcactc cttctagtaa gatttatgac
      901 gatgcatata gatttgaatg tcaagatcta gaagccctcc tttccgcaaa taatctaaac
      961 aatatagaac atgaaaactt tgttgtaaac actagattta atttagtttc tgaaaaggac
     1021 tcagatttgt tagatttgct gatagagctt actaaaaatc tagataagaa agaaaacatt
     1081 actgtactat aactagcatc ggtccatcat taagtttgga ctattagaac acttgctatt
     1141 acagaaaaag gtatgctagc cacgcttact atttattggt atcaaacaag tatcttaatt
     1201 tgaaaggaag atgatttatg gctaatttat atatagagaa caaaggagac ttgaatgcca
     1261 ttattacttt aatcgtagtt atggtaaaat caataggtaa tatcacaata ggaatgctgt
     1321 ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1381 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1441 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1501 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1561 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1621 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1681 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1741 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1801 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1861 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1921 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1981 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     2041 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     2101 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     2161 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     2221 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2281 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2341 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2401 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2461 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2521 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2581 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2641 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2701 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2761 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2821 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2881 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2941 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     3001 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
     3061 g
//
LOCUS       DAZKMK010000169         3036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000169 DAZKMK010000000
VERSION     DAZKMK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.176"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..959)
                     /gene="murB"
                     /locus_tag="ACG8V1_002847"
     CDS             complement(24..959)
                     /gene="murB"
                     /locus_tag="ACG8V1_002847"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0330858.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1032..1952)
                     /locus_tag="ACG8V1_002848"
     CDS             complement(1032..1952)
                     /locus_tag="ACG8V1_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0330859.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2065..2817
                     /locus_tag="ACG8V1_002849"
     CDS             2065..2817
                     /locus_tag="ACG8V1_002849"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0330860.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          855 a          612 c          526 g         1043 t
ORIGIN      
        1 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
       61 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      121 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      181 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      241 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      301 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      361 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      421 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      481 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      541 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      601 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
      661 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
      721 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
      781 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
      841 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
      901 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
      961 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1021 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1081 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1141 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1201 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1261 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1321 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1381 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1441 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1501 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1561 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1621 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     1681 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     1741 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     1801 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     1861 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     1921 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     1981 tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2041 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2101 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2161 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2221 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2281 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2341 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2401 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2461 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2521 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2581 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     2641 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     2701 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     2761 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     2821 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     2881 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     2941 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     3001 catatttttc gtttggttgg gcttattacc gcagat
//
LOCUS       DAZKMK010000170         2929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000170 DAZKMK010000000
VERSION     DAZKMK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.177"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACG8V1_002850"
     CDS             85..981
                     /locus_tag="ACG8V1_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0330861.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACG8V1_002851"
     CDS             1073..1288
                     /locus_tag="ACG8V1_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0330862.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACG8V1_002852"
     CDS             1306..1578
                     /locus_tag="ACG8V1_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0330863.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACG8V1_002853"
     CDS             1580..2443
                     /locus_tag="ACG8V1_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0330864.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZKMK010000171         2850 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000171 DAZKMK010000000
VERSION     DAZKMK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.178"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1759
                     /locus_tag="ACG8V1_002854"
     CDS             <1..1759
                     /locus_tag="ACG8V1_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330865.1"
                     /translation="QNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYS
                     VNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPA
                     VVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTV
                     DIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSA
                     LIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPF
                     KTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVV
                     STWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLI
                     IGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIK
                     ERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKV
                     VNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNK
                     LKDSRYVTKKEINKKNENK"
     gene            1746..>2850
                     /locus_tag="ACG8V1_002855"
     CDS             1746..>2850
                     /locus_tag="ACG8V1_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0330866.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTR"
BASE COUNT          967 a          420 c          469 g          994 t
ORIGIN      
        1 gcaaaactta ttaaaaaaag acaattctag tatactgaaa atagaattaa aaaaggagaa
       61 caacaaatct atgaaaataa gtaaaaaagc atttctttta gtagttctta tcttacttag
      121 tactttatat tcagtaaatt ttatgagaaa tgctcaagaa atttatacaa caggtgattt
      181 atcttttcat ctttcacgaa tcaaagggtt atcttctatt tttgaagggc caatcaacta
      241 tacaacattt aacaattatg gagatggact gaactatttt tatccttttc ttacaataat
      301 tcctgcagtc gttttttatg gaatttcaaa taacttaata ttaagttatg ttctatatat
      361 ttggttatta aatatttgta cgattctgat tagtttttat tatggagaaa agttttttaa
      421 aaagatagat gctgcttttt tatttagttg tttatatact tttgcaactt atcgaacagt
      481 tgatatctat tatcgatccg caattgcaga agcaatcgct cttacgatta tcccagctgt
      541 tttatattat gcttatggat tgatattcaa tagagaaaaa aaagtcatcg ctttatctgt
      601 cagtataagt ctacttgtgt atactcatgt cttaactaca ctattgtgta gtgcattgat
      661 tgcttgtatg attatagttc gtttgttttt aagcaagcaa ataaaaaacg atataaaatt
      721 tgtggtcgtg aatttagtaa aggctgtttt tttgacttgt gcattaacta gctatttttg
      781 gtatcctatg ttgcagcaga tgaattatca agaaatacac agtcccttca aaacagattt
      841 gcagctagaa gcactttcta tttcagatag tatcattcaa gcgttaaata acgatattac
      901 aacatacact atggggttat taggattggt tgcgttgatt tttcctttat tcatactgaa
      961 agaaatggag aaaaaagaaa aaatcatcta tgttggcgta gtttctacat gggtatgcgt
     1021 aactaattta tttccatggt ttttgtttca aaaaacaccg ataagcttac tccagtttcc
     1081 ttggcgtatc ttaggtatcc aaatcttttt ttcttcaatg atcataacgc ttgtttttat
     1141 gaaaaagact atgaataaaa aatattcatg gttaatcatt ggcgctactg tggtgtttct
     1201 actcgtcacg tctatagcag caaaagaaaa ttacgcaaaa gttatccaat caaaacacgg
     1261 tcatattgtt atcaatgaag aaacactccc acgttataca acatcagata tgggaggatt
     1321 atacgattac gccccaacag aagcgataaa agaaaggcct cacttagaaa aacatgaagt
     1381 aaaagtagga gaatcgtggg aaaaagggaa gtataaagct gcagataata acattactta
     1441 cacagtggca agcaacaaaa agcaaaaagt gacagtacca gtatatgctt atttaggtac
     1501 acaggtaaaa gtaaacggta aagtcgtaaa tgactcttat aaaaaacatg gcttagtgaa
     1561 tgtggatgta aatgctggag aaaataagat agaaattaca acaaaatata ctccaactgc
     1621 tctcatggct ttatggatca gtattctgac ttattgttta gtggtgtatt caagttttaa
     1681 acgatttgca cctaataagt tgaaagattc tcgatatgtg actaaaaagg agataaacaa
     1741 gaagaatgaa aataaataaa aaagtagtaa tattgagtgg attaattata gttagtagta
     1801 tatattcttt catctttttc aaaaatacac agagtattta taccactgga gatctttctt
     1861 ttcacttatc tcgaatcaag gggttatcga ctatttttac cagtccgatt aattatgaga
     1921 cattcaatta tactggatat ggtgtaaatt atttttatcc ctttctgact ttttttccag
     1981 ctgtaatgtt atattggatg acaaaaaatt tgattgtaag ctatattatt tacgtttggt
     2041 tattgaactt gtgtacaaca ctcgtagctt atcattatgg cgagcggttt ttaaaacaaa
     2101 aaaaagcggc atttttattt agttgtctct atattttctc tgcttatcgg acagtagaca
     2161 tctactatcg ttccgcaatt gccgaagcta tcgcaataac acttgttatc cctgtattat
     2221 tttatgctta tcaaatcatc tctggaaaag aagaaaagta tccgtctgta aagctagcat
     2281 tgagtatgag tttgctagtg tacagccatg tactatcaac attaatgagt acagcattaa
     2341 ttatcatttt tatttttatt cgtttggttt ctaaaggttt caagaatgca gattttattg
     2401 ctatatttaa aaaattattt agtgcggcag gaatgacatt ggttctgacg agtgcttttt
     2461 ggtatccgat gtttgaacaa atgctctacc aaaaaatcaa caaacctagc gtgacaaatt
     2521 tatatgctca tgcatctaat gtgttcgata gtctgactga agctatgaac aacgacttaa
     2581 ctacttactc aatgggacta gttggtctgt tatctttatg tattcccctg attttattta
     2641 aaaaattaac aaacatagaa aaaaagattt attatggtac gtgtttgact tggctagcta
     2701 caactagttt ggtgccatgg tatcttctgc aaaatacgcc agccaaattg ttacaatttc
     2761 cttggcggat cttaagcttg caaataatat tctcttctct gatattaacg atgatatttt
     2821 tcaaaaatag aagatacaat aagacaagag
//
LOCUS       DAZKMK010000172         2804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000172 DAZKMK010000000
VERSION     DAZKMK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.179"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1559
                     /locus_tag="ACG8V1_002856"
     CDS             234..1559
                     /locus_tag="ACG8V1_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0330867.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACG8V1_002857"
     CDS             1552..1902
                     /locus_tag="ACG8V1_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330868.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="ACG8V1_002858"
     CDS             1899..2069
                     /locus_tag="ACG8V1_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330869.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
BASE COUNT          989 a          443 c          539 g          833 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tata
//
LOCUS       DAZKMK010000173         2766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000173 DAZKMK010000000
VERSION     DAZKMK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.180"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1042)
                     /locus_tag="ACG8V1_002859"
     CDS             complement(104..1042)
                     /locus_tag="ACG8V1_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0330870.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1533..2750
                     /locus_tag="ACG8V1_002860"
                     /pseudo
     CDS             1533..2750
                     /locus_tag="ACG8V1_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          974 a          465 c          446 g          881 t
ORIGIN      
        1 ttttatattt aggaggaata atcctctgat tttttcatac gttatgtcac ctcgtaaata
       61 ttaattatac tgaattagca atttttatca aataaaactt attttacttc caaaacctaa
      121 attcacgttg ccaaaaatca atctgctttt gcaattgttt ttcgttcgct tttaaagtcg
      181 atttcattaa ttccgttaaa tcaattggag atatttctct aatcaatttt ttaaatttag
      241 tcttagtatt cttacttagc tttccccaca tactttcttc atgcaacaaa gtataaacca
      301 tagcttgctc attaattttt tctaaagtag cccacgcagg tttcaagatg tgtaaatcat
      361 taaaacaatc attccagtaa tcaaccatat ctctttttaa ttcaacttct acacgccata
      421 aatgttcaga cacaacttca acatctgcgt tatctttacg ttcttgtttt ttattataaa
      481 ttctaataaa tctatcacta tcacggacac caaaatattt tgtttctggc ttgccattac
      541 gaccataaaa aacagttttc ttaactgctt tatcagtcat tgcatagtaa tcgctcaaat
      601 catcttcaaa atcaaaagct aagtctaatc ttgtaaaacc gtcatcttcc atgtagtcga
      661 taatattttg ttttaaccaa atcatttctt catgtgtgag tttattggga ttaaattcaa
      721 cacgcatatt acgtctatcc caagtatctg cttttacttt gtcatattcg atataaactt
      781 tttcttgaag tgccttagct ttaaactttg tttgaagtat atcccaaagt cgtatttgtg
      841 gctctacact cataaagtca gatagctttt tagcattagt tttgttcaaa tttccaacga
      901 ttgtcatggc atcaaaactt aatgcgggtt gagattttcc caaagtttga ccacttaacc
      961 ggctattact taaccggcta ttagagacgg ccggtttttt tagtgagatt tctgcagcta
     1021 atttttcatc aattttcttc attttttatc gctcctaaaa taatttttgg aacgaaaaaa
     1081 ggacgagtgg aatcgcaaaa taaatttcgc ctgtatccat tcgtccattt tgttttgtat
     1141 tcaaaatcaa aaattgatat actaatcatc gaatgaataa caagtgtggt ttgttattcc
     1201 gggcgagaac gttggtagcg ttgctcgtct ttttttatgt ctttttttcg ttaaaactat
     1261 tttaaaacta aatatatctt aaagcaaata aattcaaggg gctaaaagcg ccccttaaaa
     1321 accccttgcc tctgaaaagt tttgtgtttc ctttccgtcg gtttcaaaag ctaatttact
     1381 caaaaaaata gatcaagggt aaatgcactc actgcgttcg cccttgagct attttatttc
     1441 gcaaatttta acgcatttgc cgacgaaaag ataaaacaca taaaaatata cgcagatata
     1501 tttaaaacgt cttagaacgc aaaattttac gtttaaaagc tatgcaagga ctgtatgaag
     1561 caaaattatt gagttatcca agaacagata caccatttat tacagagaac gaatttgctt
     1621 atttaaaagc gaattttggc aaatatagcg gttttttagg acttgatctt gaaatggttc
     1681 aaacagagcc tagaaagcgt tatgtggacg gtagtaaggt acaggaacac cacgccatta
     1741 tcccaacaaa acaagtacct accgaatctg cattagcgaa aatggacgat ttacaacgaa
     1801 aaatttatgc tttagtcgtt aaaacgaccg ttgccatgtt tttacctgat tacttgtatg
     1861 aagaaaccaa gatacaaacg aaagtagctg atttgctgtt tcaatcgatc ggaaagacac
     1921 caaagcaaga agggtggaaa attcttttca aacaacaaac caaagaagaa aaagaggacg
     1981 ttcaaacgtt accactcgtt atcattggcg aacgtgccga agttggtgtt aagagtgttg
     2041 aaaaagaaac gcaaccgcca aaagctttta cagagggtac attattaact gctatgaaaa
     2101 cggcgaataa aacggttgat gatgaagaag caatcaagat tttacaagaa gttgagggga
     2161 ttggaacaga agcgacaaga gcaagtatta ttgaagcgtt aaaacaaaaa gaatatatcc
     2221 aagtgattaa gaataagctt gttgtaactg aaaaaggaaa attattgtgc caggcagttg
     2281 aaagtcagca ccttttaacg agtgctgaaa tgacggctaa atgggaaacg tatttaaaaa
     2341 aaatcggtaa aagagaaggc aatcaagaga actttattac gaatatcaaa aaattcattg
     2401 ttcatttact ggaagctgta cctaacgata tagaaaaact aaatttttct gattaccagg
     2461 aacagaaaga aaaagaagca gaaaaaagta ttgtaggaaa atgtcctaag tgtggcaaca
     2521 atattgtatt aaaaaaatcg ttttatggtt gttcaaatta tcctgaatgt aagtttactt
     2581 tagctgaaca ttttagaaag aaaaaactaa ccaaaacgaa tgtaaaagaa ttactggagg
     2641 gaaaagaaac cctggtaaaa ggaatcaaaa acaaagagaa aaagccctac aatgccgttg
     2701 taaaaattgg ggaaaaggga tatattgatt ttatctcttt ttcaaaataa acataaaagc
     2761 ccttta
//
LOCUS       DAZKMK010000174         2619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000174 DAZKMK010000000
VERSION     DAZKMK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.181"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..317)
                     /locus_tag="ACG8V1_002861"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACG8V1_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(349..672)
                     /locus_tag="ACG8V1_002862"
     CDS             complement(349..672)
                     /locus_tag="ACG8V1_002862"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0330871.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            1134..2516
                     /locus_tag="ACG8V1_002863"
                     /pseudo
     CDS             1134..2516
                     /locus_tag="ACG8V1_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          940 a          456 c          433 g          790 t
ORIGIN      
        1 tttcgtaacc agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc
       61 caacgtccct taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta
      121 tttttcttct tccaaagata atagaggact ttgctgtact cttgtaccca acgataaatc
      181 gtagtatgac aaacatttat tccacgatca tatcacaatt cctgaacttc acgatagctt
      241 agattgtaac gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg
      301 cctttaaaat gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat
      361 agattttttg gaaaactttg caacagaacc gaaatgagta tagtatgaat tagtaatttg
      421 gttttcatct aagtttagct ttgatttact aaaattgata tctcgataca tgttcaatac
      481 atctaataca aatcgagaat cttctttcgg aaatggctca gaaagatcac tccaaatttc
      541 atcatcataa tgatactcgt aaccttctct caatatttcg atttttcttt cataataaga
      601 tctgttttgg tcctcaaatt tagccaataa ctcaaattga tttactagtg acaatctctg
      661 aaaactgtcc attaaatgtt tcttcctatc ttgttcatta acaaaaaatt ataagccctt
      721 acaacgaata ttatatatta aatttaattg taggtgaaca cgaattttat aaatatttat
      781 ggtgttaatg acaaatgata tccgactcct cttcatacgt tctggactat ttgctaatag
      841 attaaaattt cagcatctat ttctgaccat aaataaatga gctgccgaaa agctgccaaa
      901 tcagacttga aaataaagga tcaaaaactt ttaagtttct gaccccgtaa ctcacacttc
      961 aaaatcttac ttaaaaagga gtctgaaaga aaaaaagagt attcaaaatt tttttctaac
     1021 tagctacatc acatctatgc attgacactc aaagaaccag aataagtcat cagaaagcaa
     1081 gggggtgcgg acataatcct ggacgaaaaa ataaacttag gaggaatgtc cgtgtgtttt
     1141 cccacaaaga gcgtgtaagg gcaattcagt tatttttaaa atacgattgc tcttatgctg
     1201 caaccattcg agaattaggg tatccgtcga ttggtgcact acggaaatgg tataaagagt
     1261 atttggaatc cggagagctc catcaggagc accgaaaaaa atcaaagtat tctgaagaac
     1321 aaaaacgaat agcggtgaat cattattttg aatatggtca atgttatgca cgaaccattc
     1381 gcatgcttgg ttatcctaat agagagtcct tgcggcagcg gtgtgaagaa cttgcaccag
     1441 gagctagaaa actccgaaaa agtgcggtaa agttaacaca agatcaaaaa gaagctgtgt
     1501 tgaaaaaatt ctataaaccg caaactaata ggaaaaattt agccgaatct ggaggaatta
     1561 gtagagttac tctatatcaa tggaaaaaca cggttcttgg aaaggatttt ccattgtgta
     1621 tgactttaaa aaatcaggaa aaacaaaaag agctactttt aaaggaagta gaagacttac
     1681 aaaaacaagt acaccaatta cagctcgaaa aagcattact cgaaggagcg gctgaactgc
     1741 taaaaaaaga aaaaggcgtc aatcttcttt gtctatcaaa tcaagaaaaa acgatattga
     1801 ttgatgcctt acgaaatcag ttcaccttga aagagcttct tcaacagctc cagtttccta
     1861 aaagcagcta tttttatcaa aaacaagcac ttgagaaacc agataagtat tacaaagaac
     1921 gtcaaattaa ttatcacgat tttcaatcat aacttctgtg cgtatggtta ccgtagaatt
     1981 caccaagctt taaaaaatat gggtaaaaag ctttctgaaa aagttgtacg tagattgatg
     2041 actgaggaaa atctatttgt taaattttct agacgaaaaa agtattcttc gtatgctggg
     2101 gaaatttctc ctgcacaccc caatttatta aatcggaatt ttaaagcaaa gaagccaaat
     2161 gaaaaatggt taacagatat tactgaattt agaattctcg cagggaaaat ctacttatca
     2221 ccacttgtcg attgttacga tggtgctatt gttagttgga ctattggaac aaagccaact
     2281 tgcttcactc tatgtctaaa aagagttatt caccagataa ttcagcgtgc gaaggtttct
     2341 ttggacgatt aaaaaacgag atgttttatc aacgtgactg gaagaataca acaatcaatc
     2401 agtttataaa caaattggat gattatattc attggtataa taatcaacga attaagttat
     2461 ctttaggaag gctaagccct cttcaatatc ggaagaagca aagatatatt tgttaaaaag
     2521 gtccaaaaaa aatatccgca cccccttttt ttgtaaaaaa ctattgcaca atacaagtag
     2581 cctacggcta tcgaaatttc cataactttc gagcaagaa
//
LOCUS       DAZKMK010000175         2458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000175 DAZKMK010000000
VERSION     DAZKMK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.182"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG8V1_002864"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG8V1_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG8V1_002865"
     CDS             1320..1778
                     /locus_tag="ACG8V1_002865"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0330872.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="ACG8V1_002866"
     CDS             1937..2407
                     /locus_tag="ACG8V1_002866"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0330873.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          459 g          716 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctt
//
LOCUS       DAZKMK010000176         2423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000176 DAZKMK010000000
VERSION     DAZKMK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.183"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..2276
                     /locus_tag="ACG8V1_002867"
     CDS             123..2276
                     /locus_tag="ACG8V1_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0330874.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          828 a          426 c          536 g          633 t
ORIGIN      
        1 caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct
       61 tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg
      121 aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa
      181 aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa
      241 attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt
      301 tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg
      361 tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga
      421 ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc
      481 ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag
      541 atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa
      601 gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata
      661 aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta
      721 aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg
      781 atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca
      841 cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga
      901 cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt
      961 ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag
     1021 gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag
     1081 aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat
     1141 caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac
     1201 agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa
     1261 tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga
     1321 ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg
     1381 caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg
     1441 cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag
     1501 catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag
     1561 actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat
     1621 tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg
     1681 aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca
     1741 aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata
     1801 caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc
     1861 cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga
     1921 aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc
     1981 tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat
     2041 tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag
     2101 gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc
     2161 cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact
     2221 tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa
     2281 gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc
     2341 tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact
     2401 cggcaaaaaa ggttgtgaca aaa
//
LOCUS       DAZKMK010000177         2395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000177 DAZKMK010000000
VERSION     DAZKMK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.184"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG8V1_002868"
     CDS             316..570
                     /locus_tag="ACG8V1_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0330875.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG8V1_002869"
     CDS             571..888
                     /locus_tag="ACG8V1_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0330876.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG8V1_002870"
     CDS             1447..2382
                     /locus_tag="ACG8V1_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330877.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZKMK010000178         2300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000178 DAZKMK010000000
VERSION     DAZKMK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.185"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..>2300
                     /gene="acm"
                     /locus_tag="ACG8V1_002871"
     CDS             188..>2300
                     /gene="acm"
                     /locus_tag="ACG8V1_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0330878.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGL"
BASE COUNT          903 a          350 c          447 g          600 t
ORIGIN      
        1 atttacatta ggaaaaacct tgtaaaaaca gttttttggc atatttattt tttataaata
       61 tttgtaaaat aactatcaat tttttagtta aaagattgga aatgagtggg ggaggaattg
      121 aagaaaggat ttttgagaga tgatatagta ggttttattt ttagttaaaa taggggagat
      181 agaaaaaatg aaaaagtgta gaaatattat tttatcaatg ttatttatta ttactaatat
      241 aacatcactg attcctgttc atgtctatgc ggatgcaggc agagatatca gcagtaatgt
      301 cacttcgttg accgtggacc caacgaatat tacagatgga ggaaacataa aagtaaaatt
      361 ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat tatctttgga tcaattggcc
      421 aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa atcccattaa tgattgaaaa
      481 taaaaatgtt ggaactttaa cggtcagaaa agattctgcg caggttgtgt ttaatgaaaa
      541 tattaaaaac ctagactcag tagaaggttg ggggcaattt gagatacagg ccagaaacgt
      601 aaccgatacc ggtgaagaaa atacgggacc attcaccgtg acgagcggtg ataaaacagc
      661 tactgtaaat gtgacaaagc cagcttctgg ttcttcttct agcgtttttt attataaaac
      721 aggagacatg ctacctgaag atactaagca cattcgatgg tttttgaata ttaacaataa
      781 cggcacttat gttgaacaac cagttaaaat cagtgatgaa atccaaagtg gacaaagatt
      841 agatccaagt acgtttgaaa ttaatcaaat acacttaggt gaacagaagg tttatagagg
      901 agaagaggga atccaacaat ttttacaaga tttccccagc gctacattta atttttcagt
      961 aactgataat tacattgaaa taactatacc aaaaaacttt gtcaatctta ggaaaataat
     1021 ggtgagttat aagacgatca tagaaaaccc agaacaaata aattttgaga atcattcgga
     1081 agcttggttt aaagaattta ataaacctgc tgttgatgga gaaagtttca atcatacagt
     1141 taaaaatatt tcagcttctg gtggagtcaa cggtacagtt agaggcgaat tgaagatttt
     1201 caaatatatc aatgacacag agattggtat cccaaatgtg actttcgaat taagacgagc
     1261 agatgaacag cctattcaag gacagtctag tatcctgttg acttcgaatg aacagggaga
     1321 agctagtatc aaaggcctgc aagtaggaga ctatgttgta aaagaaaaag aagcacccaa
     1381 ttggattgat tttgatccac taagttcaaa tgaactcaaa ttctccataa acgaaaatga
     1441 tacagaggga gtcagtttac ctatttataa caagaaaaag gttacgaaca tcacagcaac
     1501 taaaatttgg aacggtggaa ccactccaag accgagtatt tattttaaat tattcagagc
     1561 aagtacaaac aattgggaac cagttcctga tgcagaaaca aaacgattag acaatggaat
     1621 aacttcagtg acttgggaag atatccaaca gtatgatgat agtggaaacg aatatacgtt
     1681 taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc ccttcaggtt atcaaaaaat
     1741 tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc atttcagtct caggacaaaa
     1801 aacatgggaa gacaatgaga accaagacgg caaacgaccg acgacgatca cagtaaatct
     1861 attagccgac ggtcaaccga tccaacacaa agaagtaagt gaaaaagatg attggcgcta
     1921 tcgtttcaca aatctgccaa aatacagaga cgggcaagaa atcatctata ctgtaacgga
     1981 agacaacgtg cccgaataca gtacgacgat tgacgggtat aacatcaaaa acagttatac
     2041 accggaaaaa acaaatatat ctataccagg tagccatata acaccgtgga aaaattttcc
     2101 agagaagaca gagagagaga aaattagaaa tctattctct caaaaattgc aaatatttgc
     2161 tcatacagga gaatccagcg aacaaagaag caattatatt agtaagagag ctgtacctaa
     2221 acagataaca aataaatcaa aaagcattct tcctaaaaca agtagtaaaa aatctagttt
     2281 tgctgtaatc attggtttat
//
LOCUS       DAZKMK010000179         2299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000179 DAZKMK010000000
VERSION     DAZKMK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.186"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..1968
                     /locus_tag="ACG8V1_002872"
     CDS             106..1968
                     /locus_tag="ACG8V1_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0330879.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1968..>2299
                     /locus_tag="ACG8V1_002873"
     CDS             1968..>2299
                     /locus_tag="ACG8V1_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0330880.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          827 a          341 c          452 g          679 t
ORIGIN      
        1 taatattagt taaaaagcta taagttgcac ttagttaagt gcaaacattt catattgtcg
       61 ttagtttaga aaacgcttat attagcaatg taaggagaga tgataatgca gcagctaaca
      121 aaaagagaaa ggcagctttt gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa
      181 aagttggctg aaaaagaaaa tgttagtgtt cggacgataa aatacgatct agatgatata
      241 agagagtggc tgaatgagcg gaagcaagtc ctatatagca aacgtagtca gggaatttgg
      301 atgaatatta gtagctctga tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt
      361 tttgaaatat ttgcagatca aagtctccga atcaaacgtt tactaattat gcttttagta
      421 acaaaagaag ctattactgg aatgaaaatg gccaacgcat tagatgtcag caaagatacg
      481 atcatgaatg atttagatgt gctggaaaat aattttctta aggaaggatt gaccttgaat
      541 cgtcaggcgc gaaagggttt ttggatcata ggagaagaac gtctagttcg attaacgatt
      601 gaagagattc ttcaaaaaga gttcacggat tatgacattt ataaattaat gtcattgtta
      661 ttgaatggcg gtgagactga atactttgaa atgtattctg ctactgctac acctatccaa
      721 gaggtattta atcaagtaat cattcgaatg cgacacttac tggaatttga aaatctagag
      781 aaactgaatt acgcggaatt actaaatatt ctgattcgag tgacaattgc taccgttcgc
      841 ttacgcaaag aggctacgat tggtcgttat caattggttg ctgaacaaga attagacaaa
      901 aacgatcttt cttatcgcgt catgcagcag gtctttgatt attatcagtt gccactgttc
      961 gaggatgaat atcattatat ctatagtgat acattcgaca cgattcacca gcaagacgtc
     1021 atgaaactag ttgagaatgt gatttataaa gtaagtaaag caatcaacca ttctttttat
     1081 caagatccac aattgttgac gaatctgtat gctcatttat ccatgcgatt atcgagaaag
     1141 aaaaaatttg tcaatgaata taatcctttt aaagaagata tcaagcagaa atacccggaa
     1201 ttatttgatg cgatcaagca agcagttcga caagaaataa gtgaacaaaa tattctgatc
     1261 aatgattctt tcgtggcata tattgcctta cactttttgg tttcctacga aagagaagaa
     1321 gatctacgaa gcgttagagc cgtctacatt tgttcaacag ggttgggtgt gactagtctg
     1381 atcaagcaaa agatcaatga agaattaaat aatatcacta tcgtggcatt tgcttcagta
     1441 ttgaatgcgc aagaaatgat tgctaccaaa gatcctgatt tggtgattag tatttttcca
     1501 attaatggca tcgatcgtcc tttcatcaaa gtccatccat tacccactga acaggacttg
     1561 aaaaaaattc aacaaataat aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat
     1621 aacttgaatc ttagagaaac gtccatcaat aaagatactt tagaagactt tagcaaggaa
     1681 ttgattttga aagcttatac gatttatgaa cgtttacttg tcattttgga agcagaattg
     1741 atccaagaat atcgagaagc ttttttgctt catgtattct taatggttca tcgtatcaca
     1801 tttgatcaac aatatatggc agaaggtcgt caagtagacc agattatttt agacaaagga
     1861 acactggtag gaaaaatcga agcagttttt gcagaaaata atttaggaat caatcagtca
     1921 gaaatcagtg cattattctc atatataaaa gaaggagcgt caagttagtg aaaatgtcag
     1981 acgaggcttt aaaaattatc gaggaaagtc aaaatcggga aaagttaaaa caagtgattg
     2041 atctaattga agagcgatta gtagaacatg ctattgtacc aaccgagttg cagtggacca
     2101 ttctaattaa tcacttgaat gagatgctga aacgttctca aagaaaggaa acgattcccg
     2161 tagtcgatcg ggatttgttc aaagaagtat ccaaagaagc cataactatt tctgatgaaa
     2221 tagttagaag tatcggtaat ttaaccgatg atgaaatata tgttttgtcg attcattttg
     2281 aaacagcaaa aaataatta
//
LOCUS       DAZKMK010000180         2183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000180 DAZKMK010000000
VERSION     DAZKMK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.187"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..279)
                     /locus_tag="ACG8V1_002874"
     CDS             complement(<1..279)
                     /locus_tag="ACG8V1_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330881.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIF"
     gene            complement(281..1708)
                     /locus_tag="ACG8V1_002875"
     CDS             complement(281..1708)
                     /locus_tag="ACG8V1_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0330882.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1701..>2183)
                     /locus_tag="ACG8V1_002876"
     CDS             complement(1701..>2183)
                     /locus_tag="ACG8V1_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0330883.1"
                     /translation="AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMY
                     EQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSK
                     LANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE
                     "
BASE COUNT          829 a          355 c          259 g          740 t
ORIGIN      
        1 aaaaatttca tcataaacgg ataaatcatc cgagaaaaat tgattatggt tagttaacaa
       61 gttaatttta cgtttaaagg aagaaccgat tttccggtga ttcacaaaaa caacatcatt
      121 aaatagccct aattctttta tacgctcact atatcttcta gcgttttcaa attcagaata
      181 aataaataaa tctccttttg attctcggta aaaattttga taaacattta atatattaat
      241 caaatgtaaa ggagtccaag caatgaaagc tgtcttcata ttaattctcc ttttttaaat
      301 acaagtaaga aatgcttccc aaataatacc agaaaacaat tgaaatgaca tcgcgcttat
      361 tgaaaagtac atgagcctcc accatttcat ctataaaaat agatagtata aaaattaaaa
      421 caataccaag aaacgcataa ttcactttac ttctaaaaaa tgttttgagt aaactaaaga
      481 tatttaacac atagaaagca tagatacata taaaagcaat tataccactt gatacaagta
      541 cttgaagaaa tccattatgc atgtttccat gtgctctttt tagttcattc acatctgtag
      601 tatcaagatc tacttttttc aaaaattttg catcgctatt cccatagaaa tcggtctctc
      661 cataaccaaa taacaaagtt ggtcttataa ctttaagcgc agctttccaa atcgtaaaac
      721 gaccagctga aatttcggag ttatcactag aatgctgtaa tattacttct ttttctggaa
      781 gtggagtttt atctgattca tttgactcta taacttctac ttttctatca atcgatacag
      841 gaattgttgc taaaaaatcc ttagcataat tagtaccaaa agaaaaatat accaatagaa
      901 ctaataatat gcttaccatc catttgattt ttacaggtag tacttctcta atgctctttc
      961 taaattttga attggcaaac aacaaaagaa ataatactaa aaaagaacaa attactacct
     1021 gggcacctct agacgaagaa agtgtaatat aaaatatttg tacgataaaa ttcatcatat
     1081 aaaaaacacg aatcgttttt gttatttttg aaattaatac actaattaaa cttaaaataa
     1141 tagaagtata tccaaacata gctccaacat ttggacttgt atagacacca aataaccgat
     1201 gttcaataaa accttgtctt gcaagtaatc ctggcgttcc tgttgggaca tgataagaaa
     1261 tcaaaaaaat aaattgatag atagatatta gcgcagctaa aaaaattatg ataatgaaaa
     1321 cattattaaa atacaccatt tttttctttt tttcctcttc actttgtgaa aagtctgcag
     1381 gaaaaattac tatcaaagta atcagcaaat atccaaaatt ataaatagat ggaattaaaa
     1441 aattttctct gttaatgatt atagttaaga taaaactaat cattgctcca atcagtagcc
     1501 aagaaaaacg cattttaaaa aaatttttct ttataaaaac ctcatacaaa actataattc
     1561 caccccaagc aaaagctatt ttcatcggga tattagttac attaaaagta ctaggcataa
     1621 aataaagcag acaaatgatt aaatttatta ctcgaaacgc atctcgactt aataaactct
     1681 tctctaaaac aggtactatt ctattcatct atatttccaa ctttcaaaat ttctttatca
     1741 attctattta atagtttatc agagtaatcc gatttaactt ttcttacaaa taatttttca
     1801 ctttctttta tttgatctat atccctttca tcaaaccatt tgttcatgga aggatcaata
     1861 atatgaatat ttgctaattt cgaagtttgt aaagtagttt cttcaatatt tttcaattca
     1921 agtggtaaat ctggtcctcc aataacattt ttatttttga acgaggaatt aaaaaaaagt
     1981 gtatgaaaaa atttttcatc tggggcaaac atacctattt taaaattcag gtagtcttgt
     2041 tgattttgtt catacatttg tattaattct gttaggcatt ctttcgacaa agcccaccat
     2101 tgagaaccat gatacaaatc aaatttatgg tgtctaattt ttgggggaag tttaatcatc
     2161 ctcagacaac gattaattat agc
//
LOCUS       DAZKMK010000181         2147 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000181 DAZKMK010000000
VERSION     DAZKMK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.188"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8V1_002877"
     CDS             complement(<1..914)
                     /locus_tag="ACG8V1_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0330884.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8V1_002878"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8V1_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0330885.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          728 a          380 c          413 g          626 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactata
//
LOCUS       DAZKMK010000182         2134 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000182 DAZKMK010000000
VERSION     DAZKMK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.189"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            691..2079
                     /gene="ltrA"
                     /locus_tag="ACG8V1_002879"
     CDS             691..2079
                     /gene="ltrA"
                     /locus_tag="ACG8V1_002879"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0330886.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          744 a          385 c          488 g          517 t
ORIGIN      
        1 attcagttaa tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag
       61 gattttttaa ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat
      121 taaatttcat atgttcatat gtacggatac attttcggac cgattaattt taaccattct
      181 atttatttgc ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt
      241 aagcagtttt aggatgtgcg ccctgcatgg gtgaaaactt ggtggtgaaa gtccactaca
      301 ggcttggcag taggaactgt tagcgaaaga caaggcggca atcgtgaggt tgtggctgaa
      361 ggaagtcgga cgcaaactcc tgaactgacg aacagaaact agatacaagg ctgaattagg
      421 tcggataagt cttcatcaca agacaaagtc caatactgcc cgaaacctat acagtaaatc
      481 tagcagttac atgggaggaa agttttcact cttaccgggg gagatctcat gagggtgttg
      541 tgggcgttga atgaaaacaa accagcacaa cgatcatggc gacatgatgg tgaatcatga
      601 gaagtcagcc gaggtcatag tagtaacgac actgaccgtt acgaaggact gaacaataag
      661 aatcttgaat aatttaggag gtgtgagaag atgcgacgac cgcagaaaac aagaaaactt
      721 ggctatcatc aaagagctag tgtggaacac gaagggtatg atgaagtgcg cagcacttct
      781 tgcggtgaac agaggaaaca agatggtgta gaaaatctgt ttgggagaat tctagatcgg
      841 aataatctca atcaagcgta tcttcaagta gtcagaaata aaggggcggc tggtgcagat
      901 gggatgaccg tcgatcaact gcttccttac ttgaaagagc accgcgagga attactatcc
      961 aatttacgcc aagggaacta taaacctcag cctgttttgc gagtagaaat accgaaacca
     1021 aacggcggtg taagaaaact aggaattccc acggttattg atcgtatgct tcaacaggca
     1081 atcaatcaag tgttacaacc gctatttgaa ccagaattct cagataatag ttatgggttt
     1141 cgcccaaatc gcagcgcaca tgacgcagtg aagaaagcaa aatcttatta tgaacaaggt
     1201 taccgatatg tggtggacat tgatatgaaa gcctactttg acaccgtaaa tcacgataag
     1261 ctgatgtatt tcattgagcg gaaagtaaag gacaaacaag tcctaaaact gatccgtcaa
     1321 tacttaatga gtggtgtaat ggaaaatgga ctcatcacaa caactgaaga aggaacgccc
     1381 caaggaggca atctctctcc cctgttaagt aacatctacc tgaacgaatt tgatcaagtg
     1441 ctagaaaatc taggacataa atttgttcga tacgccgatg actgcaatat ctatgttaga
     1501 agcaaacgag caggacagcg tgtgatgaag aaatccattc aatttctcga agaacaacta
     1561 aagttgacgg taaatcgaga aaagagtgcg gtaggaagtc cattgaaacg aaagtttctt
     1621 ggattctgca ttctgccaac aaagaaaggt attaaaattc gccctcacgc aaaagcaaaa
     1681 cagcgtgtga aaagcaagct caaacaactc actaaaagaa accgtggaag aagtatccaa
     1741 cttattttaa aagaaatcaa gcaactaatg actggttgga tcaactacta tggaattggt
     1801 gagatgaaag aattcatgag ggagttaaac ggttggttga agcgaagaat cagacaatat
     1861 atttggaaac aatggaagaa tccaagaacc aaaagaaaaa atctgattcg tttagggatc
     1921 gataaagcga aagcgtatga atggtcaaat acaagaaaag gtatttggtc agtctccaaa
     1981 agtcatattc ttcatcgatc attaacagac aaagaactgg cccgccaagg atatgaggac
     2041 atctccttga agtaccagtt cgtacactca acttattgaa ccgccgtata cgggtccgta
     2101 cgtacggtgg tgtgagaggg gcggaaggtc agtt
//
LOCUS       DAZKMK010000183         2019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000183 DAZKMK010000000
VERSION     DAZKMK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.190"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..570
                     /locus_tag="ACG8V1_002880"
     CDS             100..570
                     /locus_tag="ACG8V1_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0330887.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACG8V1_002881"
     CDS             792..1895
                     /locus_tag="ACG8V1_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0330888.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAZKMK010000184         1964 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000184 DAZKMK010000000
VERSION     DAZKMK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.191"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8V1_002882"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8V1_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0330889.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8V1_002883"
     CDS             852..1040
                     /locus_tag="ACG8V1_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0330890.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8V1_002884"
     CDS             1053..1610
                     /locus_tag="ACG8V1_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0330891.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8V1_002885"
     CDS             1642..1884
                     /locus_tag="ACG8V1_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0330892.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          717 a          305 c          404 g          538 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tata
//
LOCUS       DAZKMK010000185         1914 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000185 DAZKMK010000000
VERSION     DAZKMK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.192"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..80
                     /locus_tag="ACG8V1_002886"
     CDS             <1..80
                     /locus_tag="ACG8V1_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0330893.1"
                     /translation="PGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            77..>1914
                     /locus_tag="ACG8V1_002887"
     CDS             77..>1914
                     /locus_tag="ACG8V1_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0330894.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          716 a          234 c          384 g          580 t
ORIGIN      
        1 atcctggaaa aaatgaaatt aaatggacga aaaatattca ttcatttgaa ataatgccta
       61 ggtggtggag aaaagtatga aaccaagaat atatagtcct actgaaacag atttctcaac
      121 gaatggctta ggaattttaa aggattgtac aagatgtgga atatatgaag tagctaatgg
      181 aaaatatgaa ttagagttgg attatccttt aggaactaga tttgatgaat attttgaaaa
      241 tgactatcaa ataaaagcaa agccaaacga tcaagaagag tatcatatct tttttattga
      301 tgataaagat atagatactt ttttaaatac agtaactatt tatgctcaga gtcgtacaaa
      361 tcgacttgga agacgggtag tcactcttgt ggaagtggac tctaaaactg gtcaagaagc
      421 gatatcaatt attgaaacta aaatggataa aaaatctgac atacgacttt attctgatat
      481 tacggctgtt tctagcacaa tctttgaagc gagaaatgtt ttaaattgta ttgctggtga
      541 acaaggatca ttgcttcaat attggggcgg agaaattaaa cgtgaaccat ttaagctttc
      601 tttgttaaag cgaagaggtc gcaataatgt tggaacgatt cggtatggaa aagatatgtc
      661 tggtttgaag gtcaaattag attggacagg tataaaaaca agaattattc catatgctga
      721 tcctcaaagt gaagtaggta cgactaatcg aatttatggt tcgccagtag atagtgccta
      781 tattaataac tatcctgatg tctatacaga acatgttcag tttacagaag aacaaggagt
      841 aaaagacatt aagagcttaa ataaaatagc taaaaattac tttaaaacga tcaatcctgg
      901 ctgtgataag cctaaagttt ctattacggt tgaatttgat aagttgactg atagtgaaga
      961 agcgaaagaa tttgcgaaga ttagaaatta tggtctgttt gatacattta aaatatatca
     1021 taaaaaatat aatatttatc ttgaatcaaa ggttagtgga ctacaatacg attctttatc
     1081 tgaaaaaact ttgaaattag aagcaggaga cattcaagtt gctttttatc agcaacaagc
     1141 tgtaactatt caggataaat taaaagatta tgcaacgaat aattatatga gtgattttaa
     1201 tgattatgtt tcttcaatga ttacaggtca aggcagtgca ggaggatatg tagttttatg
     1261 gccaaaagaa aaaccttcca acatctttat tatggataat ccagatttag aaaaggcaag
     1321 agaagtacta agaatgaata aaaatgggat tgctttttct aagaaaggat ggcgggggcc
     1381 ttttaattca gcttggacat tagatagtat atttaatgcc aattttatac aaactggatt
     1441 aatcaaagca gatatctttc aaaattcttt taacaagact ggagatgtat tgaaattagt
     1501 taatggacta ctccaaattt ggaacaacaa aaagaaaata atggagttaa ctaaaaaagg
     1561 aatggaattt tggaattcta agggttcaat tggaacgatt ggaacaactg attctgttgg
     1621 taatcctttt cctggggctt ctactccaac ccctattgaa gataattctt tagttattcg
     1681 tacaaatgga gacggcaaat atattttgat ttctcctaaa gtaggaaaag gattagtttt
     1741 attaggaaat ggtaaagcaa tttattttgg agacttagat gtacaaggaa aacttacagt
     1801 taaaggaaaa gaaataattc ctggacaagg tggtggtcca tcgggaggtg gagaatctac
     1861 tggtgggtat cctagtgaat taaaaacaga tgcagaaaag agagcttgga gaat
//
LOCUS       DAZKMK010000186         1859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000186 DAZKMK010000000
VERSION     DAZKMK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.193"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..735)
                     /locus_tag="ACG8V1_002888"
     CDS             complement(34..735)
                     /locus_tag="ACG8V1_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0330895.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(751..1191)
                     /locus_tag="ACG8V1_002889"
     CDS             complement(751..1191)
                     /locus_tag="ACG8V1_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0330896.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1191..1766)
                     /locus_tag="ACG8V1_002890"
     CDS             complement(1191..1766)
                     /locus_tag="ACG8V1_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0330897.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          521 a          400 c          288 g          650 t
ORIGIN      
        1 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
       61 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
      121 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
      181 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
      241 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
      301 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
      361 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
      421 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
      481 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
      541 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
      601 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
      661 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
      721 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
      781 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
      841 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
      901 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
      961 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     1021 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     1081 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     1141 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     1201 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     1261 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     1321 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     1381 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     1441 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     1501 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     1561 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     1621 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     1681 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     1741 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     1801 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatat
//
LOCUS       DAZKMK010000187         1852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000187 DAZKMK010000000
VERSION     DAZKMK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.194"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..629
                     /locus_tag="ACG8V1_002891"
     CDS             273..629
                     /locus_tag="ACG8V1_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0330898.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            639..1538
                     /locus_tag="ACG8V1_002892"
     CDS             639..1538
                     /locus_tag="ACG8V1_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0330899.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          629 a          304 c          371 g          548 t
ORIGIN      
        1 ctttttccaa tagttgttaa aggtttttta ttttttatag tattttttgt tatttaagaa
       61 aggaataagg ttaaacaaat aacaggttgc tttttgggtc tttttccgtt caagaaactg
      121 aacgaagaaa agggaaagtt gtctcttttg tgataagaga aatcataata aacgaatgac
      181 ttactttcca ataaattgag aacagtcaga accagacaaa ttagttgcat ctgatgaata
      241 aagtgattat tctatgacat gaggtgatac agatgaaatt agaagtgaca aaagaagcac
      301 aagaagtatt gaagaataaa ttagaagcag acgatcaact tttattagat atcgaaaacg
      361 gtgacggtcc atttgcagaa agccaagtca gttgccaatt ggatacggca tttcgattga
      421 tcattgtcaa aaaagataca caaacagatc taagtcttta ttctgtagtg gctgatacac
      481 cagttggacc aatcaaaatc aaagacagtg caatacttta catggatgat ccatccacac
      541 ttgctttaga accaacttat aatagtttac aattaaaagg accaagcggc ttgagaaaag
      601 gcaacttgca agttgttcga aaagactaga gggataaaat gggaaaattg gcgatcatca
      661 gcgacctaca tgtggacatc aataaattcg gcgaatcaga gctgatgcag ctttggcaag
      721 ttttgcaaaa gcacaagatc actcggcttc atctagccgg agatacggct aataaagtag
      781 accgttgcct agctgttgtc gaatttttcg tcaataaagg attatctacg acatttcatt
      841 tcggcaatca cgagttggca gatgtgccca atgaacagat gatggaccat tttccagacc
      901 cacatttttt aaatgaacgg tatattgctt taaatgagca gaccgttctt ttagggatga
      961 atggctggta cgactatcag ttttccgaat tgaaagatta cgaggcaatc gtacgaatga
     1021 agcgtttgta ttggtatgat cggttcatcc agcgtgggaa aacagatctc gaggtcaatg
     1081 cagaaatgct tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg
     1141 tgattttatc cactcatttc gttcctaaac gggcatttat catgtatcaa tctgctcctt
     1201 atgagcgttg gaacaaatta aatgcatttt taggatcaga atcattcgga gaattacttg
     1261 accgttattc aaatatcaaa caagtagttt ttggccatac gcatcgtcgc tttgaagata
     1321 aatccatcgg tcacacgatt tacagctgca gaccttttgg ttattattat gaatggcagc
     1381 tgacccgaga ttttgtcttg aaagaacagt tgctagacag ctatgatcct atgaaattac
     1441 gtgtcctctt gcgtgcccat cacccagctt ttcaggccta tcaaacacaa catttgcaag
     1501 aggaatttga acaagcgatg acaatcgttc cgtattgaga cagcattagt aatgtgtaaa
     1561 agaaaatacc ttgtaaaaca taaggataag taagagcagt agatccattt atcgtggata
     1621 tgctgctttt tttgcggaaa ataatcagcg aatgtttatt tgatcggatg atttgtaact
     1681 cgcagagtac tagatgcaag cgtgtattct ttttttgaac gataatcaca aaaaaaaata
     1741 taatatacat tttttattta attataatta atattgattg aagtctaggc agtcaagtcg
     1801 agagtgtaaa atattttgtg taaatgaaaa aatccataca aaaaaggaag tc
//
LOCUS       DAZKMK010000188         1843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000188 DAZKMK010000000
VERSION     DAZKMK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.195"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            327..917
                     /locus_tag="ACG8V1_002893"
     CDS             327..917
                     /locus_tag="ACG8V1_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0330900.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            944..>1843
                     /locus_tag="ACG8V1_002894"
     CDS             944..>1843
                     /locus_tag="ACG8V1_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0330901.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVV"
BASE COUNT          639 a          203 c          352 g          649 t
ORIGIN      
        1 agattttaat cataaaaaac tgtactatag tgaatttttg gagatttata ttccagttat
       61 atattaatga ttgtgatact tgtactattg taaaaattcg ttaggtttaa ataactggaa
      121 atatcggaaa atgggcatta tacatcacgg acatcccttc ttttggaaaa aatgttacga
      181 tttttataag taatattgta tccggtggaa tcaacgatga agataacata tccatcgttg
      241 tagattcagt agcattgaat gatgctcttg ttgcaagaaa tccgaataaa attgttaagt
      301 ataaattgaa ttaataggag atatgaatgg aaattagcag taaagaatat ttaaaagcgg
      361 ttcttcgtat ggaaaagaat ctatatatag aatctaaagg acaacacaca aaattactaa
      421 aaataatgaa atcacatccg gactacaacg cttggcggta tactcgtcga atgagaatta
      481 catcttatta ttattcaaag agaaagaaaa atatcattta tgcgattatg tatattgtgt
      541 cttgcagaag aatgaatagt ttgggaagaa aatgtggtat agagtgtggt gaaaatgtat
      601 ttgatgaagg tgtaagattt taccatacac aaggaattgt tattaatggt aacgccataa
      661 taggaaaaaa ttgttactta tatgggaata actgtattgg aaatgatgga atcgatccta
      721 agtgtcctgt aattggaaat aatgtacgtg tatgcgtggg tgcaaagatt attggtaatg
      781 taaaaatagc taacaatgta gttgttgcgg caggagctat tgttatcaaa gattgtctag
      841 agaatggtgc aattcttgca ggtgtaccgg caaaaataat agggtatgtt aatttaacaa
      901 aagtattttt agaataattt ttagcgaaag aaggtgaata acgatgcgta tttcaataaa
      961 aagatggtca ggagcagatt ggacattgtt cacgttggca ttttttgcaa tctccatagt
     1021 tcttgtatat acttttaacg ccccaagagc aattttatat ttgggagata taattaatat
     1081 ctttgtgtta ttaaatgcac ttagctttag acgcagaaaa ggaatatatg ctagcaaaaa
     1141 tgagatgctg atagttttgt ttttactagt ttctatttgt agctcagtta ttaatttaga
     1201 aaatatagga ttgattatct gggggatacg aaataacact cgtatgttca tagtatatat
     1261 aaattgtgca acatatttaa caattaaaga ttactataaa gtattcgcta taattgaaaa
     1321 attattctgg atttcactgc cactttgttt aattgaaaga ttcatggtca cctatccaac
     1381 tggaacaatt gtaggagata tgataggtgg agttttttgg aattattcag gttgtaatat
     1441 gccattgaat ttaattttgt gtgtgacagt tactgttgtt acaatcggat atttcaaggg
     1501 agaaaaaaat ttcatccatt ttattttatg tgtttgtgca gcacttttta tgtctgcatt
     1561 agctgaattg aaagttttta ttcctgaatt atttataata tttgttgcaa tagtattcac
     1621 tttaaaagtt gattttaaag tagtgatcaa gatattatca tgcgtaatac ttgtgtcgat
     1681 agtattatcc ttttttataa caatatttat tacattgaac gataatggac aaagctatgc
     1741 tgataatttc acaattcaag gtcttttaga atatgcgtca agagattctg gatatgatgg
     1801 tgttggtgat ttaaatcgaa taactggtat ctctagagta gta
//
LOCUS       DAZKMK010000189         1816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000189 DAZKMK010000000
VERSION     DAZKMK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.196"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG8V1_002895"
     CDS             <1..828
                     /locus_tag="ACG8V1_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0330902.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETRIVDA
                     KKGKIKYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSWF
                     ESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIE
                     YGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1445
                     /locus_tag="ACG8V1_002896"
     CDS             828..1445
                     /locus_tag="ACG8V1_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330903.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1449..>1816
                     /locus_tag="ACG8V1_002897"
     CDS             1449..>1816
                     /locus_tag="ACG8V1_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330904.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          660 a          259 c          387 g          510 t
ORIGIN      
        1 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
       61 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
      121 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      181 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
      421 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
      481 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      541 atggatgcac gtgtagatct tcaaggtgta cgccatgaat cgatttctga acgtttccta
      601 tccgatctaa attatttgta tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt
      661 gaaattgaag tgactgatat tcttcaagac gatctctttt cagataatca cgaagtcgaa
      721 aaaattgaaa cagtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
      781 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
      841 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
      901 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
      961 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1021 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1081 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1141 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     1201 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1261 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1321 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1381 aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
     1441 tatagaaaat gacagatatc gtaaaaataa aacaaagcgg tgtacaggtt tatccacaaa
     1501 ctcattggaa tgctatagaa ggcaaaccaa caacggtgaa gggagataaa ggcgatcctg
     1561 gccaagcagc aacaattacc ataggaaccg tttctagtgg ttcaaccgct tctgttacga
     1621 atgtaggaac atcatctgca gctagattta attttgtttt accaaaggga gataaaggtg
     1681 atcctggaat aaatgccaca actacagctg tagctacaac tacagcaaat gggttgatgt
     1741 cgtccacaga taaaactaag ttggatggaa ttgcagctgg agcacagaaa aatccaggta
     1801 atgctacaac tacgac
//
LOCUS       DAZKMK010000190         1761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000190 DAZKMK010000000
VERSION     DAZKMK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.197"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACG8V1_002898"
     CDS             275..1753
                     /locus_tag="ACG8V1_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330905.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          645 a          246 c          314 g          556 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat g
//
LOCUS       DAZKMK010000191         1750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000191 DAZKMK010000000
VERSION     DAZKMK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.198"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            383..1633
                     /locus_tag="ACG8V1_002899"
     CDS             383..1633
                     /locus_tag="ACG8V1_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0330906.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          577 a          335 c          309 g          529 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaatgacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
     1681 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
     1741 aatttcatct
//
LOCUS       DAZKMK010000192         1679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000192 DAZKMK010000000
VERSION     DAZKMK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.200"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      115..281
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            455..1561
                     /locus_tag="ACG8V1_002900"
     CDS             455..1561
                     /locus_tag="ACG8V1_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0330907.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          483 a          271 c          350 g          575 t
ORIGIN      
        1 tttgttattt ttttctaaaa aatattaaag attgttaaat ttcactattt catttcaaat
       61 tccattgcct aatcattagg aaaagtctat catgaactcg taaatagaat atagtaataa
      121 ccttgttagg tgaggctcct atacaaacat aggctgttgc ccagaaacgt cgaaagtcgc
      181 caatgggtaa aacagaaact gttggattaa ggctttttcc aatagctaag aatgttttct
      241 ttacgttgta tagtgctaaa actcggacga tgagatgatg ctaggtcaga attcttgttc
      301 gaaaaggttt ctgacctttt ctttatattt ttgtgactga cttatttgat gagacaaact
      361 cgctcctcac ctgacaagtg gattgctgtg tgaggaggag atgcggtgaa aaaagatcga
      421 ttggtggatt ttgggtcctt atttgacttt gtccatgatc ggtcttttag aagtatacag
      481 tgccagttct tatcgtttat tgcaagccga tgaaaataca aaaagtctat tattgcgtca
      541 actaattttc atatttttga gttggagcgt gatcttctta gctcgttcag tcaaactaca
      601 ctatttactt caccctaaaa tagcaggata cggtttagcc ttatcgattt tctttttagt
      661 attagtaaga atagggatat tcggtgtcac tgtcaacggc gcacaacgtt ggatctctct
      721 gtttggcatt caattccagc cttctgaact ggcaaatctt tttttgattt tttatttaag
      781 ctggtttttt cgtgacggaa atagtagccc aaaagatcta aaaaaaccat tcctgattac
      841 agtaggtata acttttctga ttttatttca gccaaagatt gctggagcat tgatgatcct
      901 ttcgattgcg tgggtcatat tttgggcagc ggcggttcca tttaaaaaag ggatctatct
      961 aatcgttact ttttctgcat tgctgattgg agcagcaggc ggggtattat atttaggaaa
     1021 taaaggttgg cttccacaga tgtttaatca tgcttatgaa aggatcgcga ctttaagaga
     1081 ttcttttatc gatagtcatg gtgcgggcta tcaaatgacc cattcgtttt atgcactata
     1141 taatggtggc atatttgggc ggggattagg aaatagtatc acaaaaaaag ggtatcttcc
     1201 agagacagaa acagatttta ttttttcgat tattacagaa gagcttgggc taatcggtgc
     1261 attgtgtgtg cttttcttat tgttctcatt atgcatgaga atcttttgtt taagcagtcg
     1321 ctgtaaaaat cagcaagctg gattgtttct tttaggattc ggaactttgc tattcgttca
     1381 gacgataatg aacgtgggaa gtatcgcggg gctgatgcca atgactggtg tgccattgcc
     1441 atttgttagc tacggaggaa caagttatct gatcctttct ttaggaatcg gaatcacgtt
     1501 gaatatttct tcaaagatac aggccgaaga gttggcgctc tatcgacctg aaaaacaata
     1561 aagagaaact gagaaactct cagtttgaat tatcataaag tacaatcttt tatttttcaa
     1621 gcgagaggtt cttttagaaa gaggaattcg caatgaaagt atctcaaaaa atcatatta
//
LOCUS       DAZKMK010000193         1636 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000193 DAZKMK010000000
VERSION     DAZKMK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.201"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1502)
                     /locus_tag="ACG8V1_002901"
     CDS             complement(183..1502)
                     /locus_tag="ACG8V1_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0330908.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DAZKMK010000194         1612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000194 DAZKMK010000000
VERSION     DAZKMK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.202"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1612)
                     /locus_tag="ACG8V1_002902"
     rRNA            complement(<1..>1612)
                     /locus_tag="ACG8V1_002902"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          342 a          499 c          343 g          428 t
ORIGIN      
        1 tccatacatc actgtacttc cactcctgac ctatctacct gatcatctct caggaatctt
       61 actttcttaa agaaatggga aatctcatct tgaggtgggc ttcacactta gatgctttca
      121 gcgtttatcc cttccctaca tagctaccca gcaatgccct tggcagaaca actggtacac
      181 cagcggtaag tccatcccgg tcctctcgta ctaaggacag ctcctctcaa atttccaacg
      241 cccgcgacgg atagggaccg aactgtctca cgacgttctg aacccagctc gcgtgccgct
      301 ttaatgggcg aacagcccaa cccttgggac cgactacagc cccaggatgc gacgagccga
      361 catcgaggtg ccaaacctcc ccgtcgatgt ggactcttgg gggagataag cctgttatcc
      421 ccagggtagc ttttatccgt tgagcgatgg cccttccatg cggaaccacc ggatcactaa
      481 gcccgacttt cgtccctgct cgacttgttg gtctcgcagt caagctccct tctgccttta
      541 cactcttcga atgatttcca accattctga gggaaccttt gggcgcctcc gttacctttt
      601 aggaggcgac cgccccagtc aaactgccca tctgacactg tctcccacca cgattagtgg
      661 tgcgggttag agtggccata acgcaggggt agtatcccac cagcgcctcc atcgaaacta
      721 gcgttccgat ttctacggct cctacctatc ctgtacatgc ggtacagaca ctcaatatca
      781 aactacagta aagctccatg gggtctttcc gtcctgtcgc gggtaacctg catcttcaca
      841 ggtactaaaa tttcaccgag tctctcgttg agacagtgcc caaatcgtta cgcctttcgt
      901 gcgggtcgga acttacccga caaggaattt cgctacctta ggaccgttat agttacggcc
      961 gccgtttact ggggcttcaa ttcgtacctt cgcttgcgct aagcactcct cttaaccttc
     1021 cagcaccggg caggcgtcag cccctatact tcatcttacg attttgcaga gacctgtgtt
     1081 tttgataaac agtcgcttgg gcctattcac tgcggctgat cgtatgatca gcaccccttc
     1141 tcccgaagtt acggggtcat tttgccgagt tccttaacga gagttctctc gctcacctta
     1201 ggattctctc ctcgactacc tgtgtcggtt tgcggtacgg gccgctgttt tctcactaga
     1261 agcttttctc ggcagtgtga catcaggaac ttcggtacta ttatttccct ccccatcaca
     1321 gcttgtcctt aagaaaagaa gcatttgact cctcttcaga cttactgctt ggacagacat
     1381 ttccgatcgt ctgcattcct tagcctcctg cgtccctcca ttgctcaaac aaaaacaacg
     1441 ggtacaggaa tatcaacctg ttatccatcg cctacgcctg tcggcctcgg cttaggtccc
     1501 gactaaccct gggcggacga gccttcccca ggaaacctta gtcattcggt ggacaggatt
     1561 ctcacctgtc tttcgctact cataccggca ttctcacttc taagcgctcc ag
//
LOCUS       DAZKMK010000195         1609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000195 DAZKMK010000000
VERSION     DAZKMK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.203"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..531
                     /locus_tag="ACG8V1_002903"
     CDS             <1..531
                     /locus_tag="ACG8V1_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330909.1"
                     /translation="DENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDI
                     FAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPIS
                     TISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILL
                     PENWAELRKLVEYGII"
     gene            518..>1609
                     /locus_tag="ACG8V1_002904"
     CDS             518..>1609
                     /locus_tag="ACG8V1_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFQ0330910.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIG"
BASE COUNT          523 a          207 c          261 g          618 t
ORIGIN      
        1 gatgaaaaca gtaaattgat gaaaaacata caatcaatct ttgggataga agaagttttt
       61 gtcggagata aatgtatctt ttttgatcaa cctttattat ctgatggtta tggcatagat
      121 gaaacagata ttttcgctaa agtaaatagt atattttctg gaaaaaaaga aactttaatt
      181 aagcttcatc ctagagcaag gacaaatcgt tatggtgaaa aaaacatatt gaattccagt
      241 ttaccgtttg aagtcttgct acttgaaaat gacttctctt caacactttt gattagtcct
      301 atatctacaa tttctttttc accatctatg atgtttgaaa agacgatcaa ctctattatg
      361 ttggctaaat taattttaaa tgaatataca gcattatctg aagataaaaa agaaacattg
      421 acaaatattg ttcttttctg tgaacattat aaccgtctat cagaagaaaa aattttactc
      481 ccagaaaact gggcagaatt aagaaagttg gtagagtatg ggattattta acacactttc
      541 caagtcaaaa ttagttaagt ccagtttctg gtatactata gggaattttt ttattaaggg
      601 aatttcattc ataactattc ctatttttgt aaatattact actcaagcag aatatggttt
      661 gattaataat ttcacttcat ttgtatcaat tttttcgatt cttgttgggc taagcctaaa
      721 ttcggcaatt aataatgcaa gttttgacta taaagatgac ataaatgaat ttatgtcatc
      781 ggtactttct ctagctacac tttcgttaat tgtgttctta ttaataggga acatatattt
      841 tttgttttca aatgcttttt ttgatatgaa ccaacttgta tttaatttga tgttattaca
      901 aagttttgct agttttgtaa ttagttatat ccagtcttat tttacgatta actttaacca
      961 ttttaaatat ttgattttat cttttatatc tacttttttg aatattgcaa tctctgtagc
     1021 tttgatgcag acattatatt ttgatgataa atatgttggc agagtagtcg gcggaacact
     1081 gtcatttgtg ttgataggtt tagtaattta tttaagaata atgataaagg gtgctcattt
     1141 ttttgataaa aaacagtgga gatacgcttt aaaaatctct ttgccgctaa tcccacattc
     1201 cttagcaaat atagttttag cacaattcga tagaattatg attaattctt atttaggtcc
     1261 tgctgcgggg ggaatatatt cctacattag taacataggt attattctaa gcgtaatatg
     1321 gatgtctact aataacgcct gggttccatg gttctatggt gagatgagta aaaataatga
     1381 aaaaaaagtg aagaaagtta gtaactatta tttagtagtc tttactttga ttacgatgat
     1441 tgtaatggta gtttcgattg atttagcaaa atttatggct ccgaaagact attattctgg
     1501 gttatcttta gtggttccga taaccttagg ctatttcttt cagtttttat atagtttacc
     1561 agttaatgct gaattttatg agaaaaaaac atcgtttatt gcgattggt
//
LOCUS       DAZKMK010000196         1530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000196 DAZKMK010000000
VERSION     DAZKMK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.204"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..923)
                     /locus_tag="ACG8V1_002905"
     CDS             complement(<1..923)
                     /locus_tag="ACG8V1_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0330911.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYV"
     gene            complement(920..1144)
                     /locus_tag="ACG8V1_002906"
     CDS             complement(920..1144)
                     /locus_tag="ACG8V1_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0330912.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1141..1434)
                     /locus_tag="ACG8V1_002907"
     CDS             complement(1141..1434)
                     /locus_tag="ACG8V1_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330913.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          433 a          319 c          253 g          525 t
ORIGIN      
        1 acatagactt tcttattagg gaagttacgc atgttaaaag atacatgata gccaacattt
       61 ccagaagtat tataagcttg atttacatct gatctataaa ttccatcagc tcttactcga
      121 gctaattctt tcccagtatt gtaatccata atgaagatat actcgtattt atagttagca
      181 atgtgccatc cagctacatg caaatttgca ttttcgattt ctccaaactg atcaatgtgg
      241 gcgtaattcg ttccatctgt cagtgtagga ttagctgcac cagctcgtgt tggatcaatg
      301 acaggcttgt tttcagaagt tgttggattt tcatcggtaa atccatgagc taaatcatat
      361 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      421 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      481 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      541 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      601 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      661 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      721 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
      781 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
      841 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      901 gtaatagtaa ttttcttttt catttgtttc ctccttcttc gctttcagcc gagaacattt
      961 tgtaggttcg atttgataca cccaacacac tccctaaaaa cgcgccaaaa ccagtaatga
     1021 tgacaacaca gatatctgtg tactgccaat tgagcgcttt accaactaac cccacgaaag
     1081 tagctagtgc gggaataatt accagtgcga accattttag tacttcgaac gttttattat
     1141 tcattttctt ctctccctaa acaaagtttt gatttgttgc gtgtgttcca ccaatttttc
     1201 tgtatgtgta tctaatcttt catcgtgttt ctttagttct tcatgaatca tcaatcgatc
     1261 tgatttgctc gattctaaat ctttagtcag caaatctaaa ttgtggctta cttttgaaag
     1321 agtctcagta attttcgaga aagatgcagc aatcggtcta attactaata aaatcaaaga
     1381 aacgatagcg gttattgatc ctgctatcat tccccattcc cctaaattaa tcatgtgaca
     1441 actcctttac cttaaataaa aacgcatcaa ttaagatgcg ctcttatctt tattaatgat
     1501 tttatctgct tcttcgtctg taatgcataa
//
LOCUS       DAZKMK010000197         1528 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000197 DAZKMK010000000
VERSION     DAZKMK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.205"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8V1_002908"
     CDS             complement(99..1229)
                     /locus_tag="ACG8V1_002908"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0330914.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          434 a          309 c          240 g          545 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaat
//
LOCUS       DAZKMK010000198         1525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000198 DAZKMK010000000
VERSION     DAZKMK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.206"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..1422
                     /locus_tag="ACG8V1_002909"
     CDS             190..1422
                     /locus_tag="ACG8V1_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0330915.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          502 a          284 c          321 g          418 t
ORIGIN      
        1 ttttttgata tgcaaaatta gtttttgatt ttcttcacaa gctgaaatag caagtagtaa
       61 catatatcat gaaacttaac cttaaaaaaa taacctcctt tttttattta aagcctcatt
      121 gtttgaatca taattaaatg gtatcatgct aatagttacc agaaaaaatg gtgaggagga
      181 atttttatta tgttatctga ttttaatgaa cttctaaaaa aatatgcacg cttaattgca
      241 gaaactggcg tagccacaga aaaaggacac acagttgttt tacagatcag cgtggaacag
      301 gcaccccttg ctcgattgat cacacaagaa gcttataaac taggagccgc tgaggttatc
      361 gttcaatgga ctgacgacca gatccaaaga gaatttcttt tacatgctgc aacggatcgt
      421 attgagaatg taccacaatc taaaatcgat caagcggatg aatggcttga aaaaggagca
      481 agcaggatca gcgttgtttc tgccgatccc gatgcatttg ctggtgtaga tagccatcga
      541 gtagcgtctt atcaatcagc gaatggcaaa gcactaatga atttacgcaa ggcaactcaa
      601 gcaaataaag tcagttggac tgtcgtggct gcggctggta aacaatgggc agcaaaagtc
      661 ttcccagaac tgccagaaga aaaacaagtt gaggcattat gggatcagat tttcaaaacg
      721 actcgtgtct atgaagaaga ccctgtctta gcatggaaga agcatgatga aaaattagca
      781 aaaaaagcag aagaattgaa tcgagaacaa ttttctgccc ttcactatac tgctcccggt
      841 acagatatca ttatcggatt gcctaaaaac catctttggg aaggcgctgg aagctataat
      901 gctcgtggag aaaaattcat ggcaaatatg ccgacggaag aagtgttcac agcacctgac
      961 agtcatcgag tagatggtta catttcaagt acaaagccac tgagttatgc tggcaccatc
     1021 atctcgggta tgaaatttac ttttaaagat ggaaaagttg ttgatttttc tgccgaacaa
     1081 ggagaagatg tcttagcaaa actgcttgat acagatgaag gtgcgcgtcg tttaggagaa
     1141 gtcgcacttg tacctgaccc ttcacctatt tctcaatctg gcatcatctt tttcaataca
     1201 ttatttgatg aaaacgcatc gaatcactta gcactaggtt ctgcttatgc cttcagtgtg
     1261 aaaggcggta cagaaatgtc cgatgaagaa ttagcagaag ctggattgaa tagaagtcaa
     1321 acccacgtag atttcatggt aggatctgac aagatggata tcgacggaat ccgtgaagat
     1381 ggctctacag taccgatttt ccgaaatggc gattgggctt gagtatactt tcgcacacat
     1441 gcaaaaaata atataaagca tagaataaag gatctaagct attggcttag atcctttatt
     1501 tcgctaaaca accccaccgt ttttt
//
LOCUS       DAZKMK010000199         1522 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000199 DAZKMK010000000
VERSION     DAZKMK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.207"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..639)
                     /locus_tag="ACG8V1_002910"
     CDS             complement(<1..639)
                     /locus_tag="ACG8V1_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0330916.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTL"
     gene            complement(629..>1522)
                     /locus_tag="ACG8V1_002911"
     CDS             complement(629..>1522)
                     /locus_tag="ACG8V1_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0330917.1"
                     /translation="INIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSI
                     IQTWIDLKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNI
                     VTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWN
                     ALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMT
                     NLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIK
                     TELTRQMKRDAGAVGGTLYAT"
BASE COUNT          490 a          275 c          177 g          580 t
ORIGIN      
        1 taaggtgacc tgatcctgat gttctaaatt accatctaat tttcgataag cctcttctaa
       61 tttagaatct ataaataatt ctctgtccaa cgatttcaaa tcatatttat ccttattaat
      121 ataaaaagaa gcatctccag aaccactcaa tttaataagc ggttttgaag aatacttctc
      181 tgggttataa atttcaaaag tttctgtttg tcgaatagta aatctaccat tgtaattttc
      241 cttaaatggt cgaatactca ctgtaaattc aaaagggaca atatttcctg tttttcttgt
      301 tcctttaaat tcaggtcctt ctattactac agcttgataa atatattgtt catcatagta
      361 aagaataaaa tcactataag acgacatatc taaccattca gtgattcgat cttcccactc
      421 ctgcaccata tcaattgaag gtgctttata gtaacactca atctttctag tcacattttt
      481 gtaatagcct ttatcaataa tgatagaatc atttccttct cgctctctca attcaattac
      541 tcgactagct gaaacagaag caggcctgtt ttgaatatac acattaaatt cagaagaata
      601 atgttgatta ataaaaaatt gtcctctctt aagttgcata taatgttcct cctactgcac
      661 cagcatctcg tttcatttgt cttgtcaatt ctgttttaat ttttgtggta atcttccgaa
      721 ccattgaatc aggtaaatct ccataaacat ttaaatgtaa atgaatttct tttgtttcac
      781 tagcagatat attttgcttt gtttgtgtaa ttggtaggtg acttattcca tttactacag
      841 gttgaacaga cggtgttttt actaaatttg tcatggtttg atctaactta ctttgctctt
      901 tatcaatacc aacgataata ccttgaacaa tatttttacc aaccatatca cgcatccatc
      961 ttgatggaga atgaatatct aaagctcctt taatccaatt tttgatatta cctgcaatac
     1021 ctttgatagt atctttcaaa gcattccact tttcttttac accatttata agtccatcaa
     1081 tgatgttctt gccaatttca aataaattga cttcccttaa cgaattaaag atttctttta
     1141 cccgattaat agcatttgaa acaccatttt ttagatttgt ccatgcattt tcagcagaat
     1201 taacaattcc agtaacaata ttccagaaag agtccttaat gttattccat gtattaatca
     1261 tggtattttt tatagaaatc cacgtattgt atgccgtatc tttaatattt ttccaagtat
     1321 ctttgaaaaa ctgtttaata ttattccaag tagtaatggc attatatttt aaatcgatcc
     1381 aagtttgaat aatagaaaac ttcaattcaa tccatttctg aattgcaaaa tattttatat
     1441 caatccaaag attaataaaa aaatacttta tgtttaacca aatcgattta gcttgattta
     1501 ccacttcatt ccaaatattt at
//
LOCUS       DAZKMK010000200         1515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000200 DAZKMK010000000
VERSION     DAZKMK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.208"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..428
                     /locus_tag="ACG8V1_002912"
     CDS             30..428
                     /locus_tag="ACG8V1_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0330918.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            412..1107
                     /locus_tag="ACG8V1_002913"
     CDS             412..1107
                     /locus_tag="ACG8V1_002913"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0330919.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          570 a          219 c          307 g          419 t
ORIGIN      
        1 caaatgatat tttacgagga ggaacagaga tgacgatctt tgaaacggtt gtaacagatg
       61 ttggtataga agcagaagta ttcaggaaag aaaaaataat gattttattt ggagatgaag
      121 caccggaaac attaacagat tattgttata agattgaatt gaatcaggta agaaaagaaa
      181 taacttgtga tatgaagctc tgttttgata aacaaatgta tgtaatcaca gcagtaggag
      241 atgttgtcca aactaatttg aatgaattag gacatatcac gataaaattt gatggatctc
      301 atgagccgga acttccagga acaatgtatg tagaagataa ggaaatgccg catttaagaa
      361 aggggacaat cattaaaatc gatcaagagg atcagaatgg gggaaagcta tatggaaatc
      421 atgcttgata cagcaaatac aaaaagtatt tattcttgtc aacagttttt gaatttaaca
      481 ggcgtaacaa caaatcctac aattctgaaa aaagaaggag cgtcaaactt cttctcgcat
      541 atgaaagaaa tcaaaaaaat aatcggacag attcctatcc atattcagac aataggaaga
      601 acggaagaag aaatgataga ggatgctcga gttatcatag aagaactggg aaaagatacg
      661 tttatcaagg tgccggtgaa tcaagcaggc cttcaagcga tcaaaaagtt gaaaaaagaa
      721 ggttatttga ttaccggtac agctatttac acagaattac aagggtattt agctattaat
      781 aatggagcag attatattgc accttattat aacaggatgc aaaatcaagg cattgatgct
      841 atgaaagtga ttgattcgtt gcggactgag atcgaacgta cgcaaagacc aacaaaaata
      901 ttagcagcta gtttcaaaaa ttgtgaacaa gtcactacag ccattcgatc tggagctcat
      961 gcgataacgt taggtaaaga gctactagat caagtattag agatgccctc gattgataaa
     1021 gcagtgaggg gcttccaaga agattgggtg caaactttcc aagcagaaac gattggtgaa
     1081 ctaaaaaagg tcgctgatta cttctgaatc aatttctcca taaaataaac gatagccttc
     1141 tcgaaaactc gaggaggcta tctgacgatt caatattcat taatttaaag tttgggtgac
     1201 tattactaac tattacttat ttctcattta aacgatgtat tccctaacct ctaatacatc
     1261 cttgtaactc tgaagttacg ccagttttga ataaatttca taaaaacaaa tgaaaagtgt
     1321 cgcataaaaa tatctttcgt gtttctctag tataacagaa taagaagaac aagtccagaa
     1381 gggatatttt tagtcttttt taaaataact tttcagtcga gtttgagttt atgatagttg
     1441 tttttgaagc gatacggcaa gtgaaaaaat agggaagcat gaaagaatct acttaatggc
     1501 gcattgattc atgtg
//
LOCUS       DAZKMK010000201         1482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000201 DAZKMK010000000
VERSION     DAZKMK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.209"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG8V1_002914"
     CDS             <1..828
                     /locus_tag="ACG8V1_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0330920.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1448
                     /locus_tag="ACG8V1_002915"
     CDS             828..1448
                     /locus_tag="ACG8V1_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330921.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          529 a          212 c          337 g          404 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgtc aatcagtcat atgtagatca aaaattccaa gaagctgtaa
     1381 atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa aaaattgggg
     1441 aggtatagag aatgacagat atcgtaaaaa taaaacaaag ca
//
LOCUS       DAZKMK010000202         1481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000202 DAZKMK010000000
VERSION     DAZKMK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.210"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..769
                     /locus_tag="ACG8V1_002916"
     CDS             <1..769
                     /locus_tag="ACG8V1_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0330922.1"
                     /translation="VDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELA
                     DNIGISEVLLSYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKI
                     VMGKRIREVRKNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVT
                     VPYLMYGEDYKNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSIS
                     KWSIDRYENGRDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT          556 a          168 c          300 g          457 t
ORIGIN      
        1 cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag atgaggaaga
       61 taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata agttacttaa
      121 caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg tgttaaatag
      181 aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat taagtaatga
      241 aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata tttagtttat caaaagacga
      301 taaaattgtt atgggaaaaa gaatacgaga agtccgaaag aaccgggaag agacgcttga
      361 aaaatttggt aaaaacttta ccatctatac aggcaaaaat gttataagta gatgggaaaa
      421 aggtattaat attccagata ttgaaaaact aatgaatatt gcctatcttg gtaaggtcac
      481 tgttccttac cttatgtacg gagaagatta taagaatata cttccaaaag atgaaagagt
      541 tagcgatttt aaaaagataa attcttttag tatgggatta aggatgagga aaattaggaa
      601 agattattat ctagaaaggg aggagtttgg aaagcttttt tctccttcaa tttcaaaatg
      661 gtccatagat aggtatgaga atggtagaga tatacccaat actaatcgaa ttatccaata
      721 tgcttatata ggaaatcttt ctttagaatt tttgatttac gggatataaa aatagtttac
      781 gtcgatactt attaagttat cgacgttttt tattatagaa aaaatttata aaaggaggtt
      841 tcttgatatg ataagaaaca aaaagggttt gttgaaagtt gctttactgg cagctttttt
      901 ctaagtaact ttttgacaag ctcaatggca acacaagcgg aagaagtaat acaagaacta
      961 actgttatga ataacagcta gataaaatgg gctatgtatg ttatccaaca atttagtaat
     1021 cactagaagc ctagcgaaag ttcgcttgat aagttataga tgagtattga tttgtagtgt
     1081 ttccaaatca aaagggtaca gagccacatg aaatatgttg tcctctgcag cgtcaggatg
     1141 ggaatccata ccatggagat tcggagatct gtgagggaat acttacgtgg ttaatatgtc
     1201 agttgtgttg gacgtagtaa tggtcatagt gggtagattg actgaattct tatatgagaa
     1261 aatgaaacca acaagaagat agggttattt atacagaatt ggggatcttt tctggaaaag
     1321 taatcggata atgcgagatt cgtacggtct tttttaaatt tctctaaaaa aaacggcatc
     1381 gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata aaaaatgatt
     1441 ctatttatct ttttacatct atcgtttttt caagtagccg c
//
LOCUS       DAZKMK010000203         1470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000203 DAZKMK010000000
VERSION     DAZKMK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.211"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..1200
                     /locus_tag="ACG8V1_002917"
     CDS             31..1200
                     /locus_tag="ACG8V1_002917"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0330923.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          520 a          270 c          240 g          440 t
ORIGIN      
        1 taaaaaagaa aggagactaa ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga
       61 aaatattcat ttttaatgat cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga
      121 actattcttg gatggcaatt ttacaataat gaacactctc agatagttgc ccatgctcaa
      181 tcagctcaaa aaaagttgat actgactacc aatgaacaac agctcgttga aaaaatcgat
      241 caactattaa gaaaaaatga ttatattggc tcaatttacg taaagaaaaa taatcgcatc
      301 atcatggaaa aaggctatgg ctatgcgaat aaactcacgc aacaaccaaa tgatccttct
      361 ctttattatc agattggttc ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa
      421 gtcgagcgtg ggcacctttc tttagatact aagctggctc aattctatcc acaaattgct
      481 ggcagccagc aaattactat ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact
      541 gcttctccaa ccataccgat gactgatgaa gaagtcgttc aatttgcgat tcagcactta
      601 aaatttacag attatcagac ctctcattat gattcattga actttaccat attgactgga
      661 attttgatca aattaacaaa tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa
      721 cctttaggat tacagcatac aaaattttat gaccaagtca aaaattcatc caatcatgca
      781 ctatcctatc aaatgtctaa agaggatgat tactttaaac cactgacaga atctgaaaca
      841 gagatccata acgaattagg gactggtaat atcagtatgt ccgtctatga tttaaatacg
      901 ctgtttacac aagttttatc tggaaaacta gtttccaaag acttactctt ctcattatgg
      961 cgtgaaaatg cgaatggaca tccatatagt ggtggggtat atagcggaga taattatatc
     1021 ctgtcgcaag gaaatatcga ctcttttgat acatctgcta tatttagcaa agattcacag
     1081 gatgctatca tcatggaaag caatataaaa gctgacaaaa ctatcaaact gcctactgct
     1141 gatctaagaa accaaatcta tgacctgatg gaaggaacta ctaagttggc aaatagctga
     1201 tatgatgaaa agatagcaaa tactacttct ttgaatagca tttgcttaaa aaaagtactc
     1261 gactaaacgt aggaaagagt gtgggacaaa tctaaaaatc aatttttgct acagacttta
     1321 attccaaata aacagaacag aaagaggtgt tcttctttct gttctgtcca aattattcta
     1381 agatttctct ttaccaacat tatttgacaa agggccctat cggctctata ctttttgatg
     1441 ttggtgaact tcaccaacat cttttttgta
//
LOCUS       DAZKMK010000204         1458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000204 DAZKMK010000000
VERSION     DAZKMK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.212"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..187
                     /locus_tag="ACG8V1_002918"
     CDS             <1..187
                     /locus_tag="ACG8V1_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0330924.1"
                     /translation="LLINLYYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHR
                     RYDYIKYTFVDPERYKD"
     gene            complement(223..594)
                     /locus_tag="ACG8V1_002919"
     CDS             complement(223..594)
                     /locus_tag="ACG8V1_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330925.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(596..997)
                     /locus_tag="ACG8V1_002920"
     CDS             complement(596..997)
                     /locus_tag="ACG8V1_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330926.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(1011..1418)
                     /locus_tag="ACG8V1_002921"
     CDS             complement(1011..1418)
                     /locus_tag="ACG8V1_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330927.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          467 a          256 c          190 g          545 t
ORIGIN      
        1 cttattaatt aatctctact attctaataa gaactacttt aaaatccctg ctgcgaaaac
       61 tagaatgaag aaagacgtat attttttgtt tgatatcgtg actgatgtgc cggacgcacg
      121 tagcgatcat cggcgttatg actatataaa gtatactttc gttgatccag aaagatacaa
      181 agattaaata aaaaaataac aaacaaactc gcttgaaatt cattaggtga gtttgtttat
      241 tattttttct gcttctcgat tcatcagcgc catatctgtt ttcgtaatgg tttcattagg
      301 aatatagtca gcacattttc ttttctctaa cattctatac ccactattat gtattcctaa
      361 ggtattcaat tccttaactg ttgaaaacaa attccaaacg gtttggtggc ttccttttcc
      421 gctacttcca ttataaataa tatcataatt ttctttcaaa tactcatcag attttttaaa
      481 agcactgtag tatgctcttg aaacagcagt tcttctataa gattctttag aattatattt
      541 atcatctgat tcaatttgtt tggaaataga taaatattca ttaaagtcaa acattttatt
      601 cccccaaagt tgctactaca gatttcccca tatatttata gtcagataat acattatcga
      661 gagtatccaa aacatcgtaa tctgaggaaa acaattcaga tgttgttttg ataacaaaga
      721 aatacaagtt taaatcttca tcatatttta tattgatttc agcttttctt tttaaaaccg
      781 tatttatcac atcaaaaatt ttgccatcat tacttgaaat tgtgttaatc tcattttttg
      841 tagtaattac aagttgatta tcatttttag ttttcggagt agaacttgca tccacaacaa
      901 ctggagatgc tagaattacc gatgcgcaag caaaaatcgc agcaaaattt attaagctac
      961 cactccgatg tgtatttgat ggtactatat caagcatccg tatcgtctcc ttaatttttt
     1021 tccactggtt tgttgacctt gatatcaaag atttgccttc caatctcagt tccattaaat
     1081 ttaacaacag cataatgctc tcctgcttct tcaaccaaga catttctggc gtttatatta
     1141 aagtttatat cgttaacttg aggaggcaag cttaccgata cttcacccgt agaaaaaatt
     1201 tgtttttcat tcttgtttaa aacttcaatt tctactattt ttatcttttc tgtatcctct
     1261 atctcagaca ccacagctgt tacagaaaat gataaagcag taggaattaa aggcgcatta
     1321 atagaaagag ctggattaat tactttatta ataacattag tgccaaattg ctgactttct
     1381 acatcttctg aaattattaa ttgtactcta taagacattg atttctccta atttttacat
     1441 ttatttaata aatttatg
//
LOCUS       DAZKMK010000205         1364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000205 DAZKMK010000000
VERSION     DAZKMK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.213"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG8V1_002922"
     CDS             82..>1364
                     /locus_tag="ACG8V1_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0330928.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZKMK010000206         1319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000206 DAZKMK010000000
VERSION     DAZKMK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.214"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACG8V1_002923"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACG8V1_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG8V1_002924"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG8V1_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          470 a          218 c          179 g          452 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatata
//
LOCUS       DAZKMK010000207         1309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000207 DAZKMK010000000
VERSION     DAZKMK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.215"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..574)
                     /locus_tag="ACG8V1_002925"
     CDS             complement(<1..574)
                     /locus_tag="ACG8V1_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0330929.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCT"
     gene            925..1224
                     /locus_tag="ACG8V1_002926"
     CDS             925..1224
                     /locus_tag="ACG8V1_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0330930.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          473 a          187 c          216 g          433 t
ORIGIN      
        1 gcgtacaatt ttttagatgt tccgagcgta cttcaatcat ttcaaaggag ttgctactta
       61 aatcgatatt ttttaaatca gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa
      121 acattatttt agtgagggaa cagtctacaa aatctgcgct tgataagaca gagtgctcaa
      181 aggtactttc ttttatgttt gaaccagata aatttacata gtccatttta caattctcga
      241 ataacgtgtt cccaatataa ctttccatca atttagtacc tgttaattta caattgacaa
      301 atttcacgcg atgcaacatg ctttttgtca atatacagcc tgaaaaatca cagttttcga
      361 atatacagtc ggtaatctcc attctttgaa aatcattgga agcaaaaaat gcatacttga
      421 aaatagattt gtaaataatt ggatggcaga ttatttggtt ttcgatgacc tctttttgtg
      481 aaaggatatt atacagttca aaatcttcgt tttcatagat atgaaaaaag gatcctttat
      541 tcaagcttgc ttcattaact ttaggaaaaa tcatatttaa ttacctccat tttcataaag
      601 tcatattaac aaaagctaaa ccaacttaca ttagcagaaa atgagatagg ttttcgcctt
      661 tttcgataac gatagtgtcg ctatctcttg aaacgttctt agaattgccc tgagagacac
      721 aaattaggcg taattctaat aaaaatctta gaataaaaat ttttactgat ttaatttaga
      781 atgtttttaa aataaaaatc gttgccataa cagtgtattg aaaggtttta acaatccagt
      841 aatgatttaa aaaaatctag atgtagcgta tactttgttt atagaatgta gtgggaagaa
      901 atatattttt gggaggaaat cgtgatgtct aaaagtgaag aaatagaaaa catggtacta
      961 aaattatatg atctcgtttt aaatccagag atcaaagaaa aggaacgtat acttttgata
     1021 gacgcaaaga caggcttaga aaaaggtcag tattatccta aagtcataaa taatttggaa
     1081 cgtagtttac gtccgttagc aatcagggga gaattgtcta aaccagtatc accgttttat
     1141 atggagatat caacaatagg aaaatttgaa aaagaattag gtcgaggaat ggcttcggca
     1201 ccgattactt ttgggcattt ataaaaacga gttattcatc tcaatgaatt aaaaagtata
     1261 gtcaatctca taatgcaggc taggttcatt tatttgaaca gagtctgta
//
LOCUS       DAZKMK010000208         1305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000208 DAZKMK010000000
VERSION     DAZKMK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.216"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..1103
                     /locus_tag="ACG8V1_002927"
     CDS             96..1103
                     /locus_tag="ACG8V1_002927"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0330931.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1115..>1305
                     /locus_tag="ACG8V1_002928"
     CDS             1115..>1305
                     /locus_tag="ACG8V1_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFQ0330932.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLK"
BASE COUNT          514 a          162 c          244 g          385 t
ORIGIN      
        1 ttttgacatc attattgata attagtttag ttatatattt tttaaagatt aagaatcgaa
       61 ttatagatga ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag
      121 taactggcgg aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac
      181 caaaagctat cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc
      241 tctcaaatca cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg
      301 attatgcatg tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag
      361 caagtgaata taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa
      421 tagatgcagc gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag
      481 tgagtcctac taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg
      541 ccaatcagta taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca
      601 tggggtcaag tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata
      661 ttactttgac agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat
      721 tagttctgtc tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg
      781 caatcaatat tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg
      841 aaaaatatag aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag
      901 agctaatgac tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc
      961 caccacacta tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg
     1021 catactcttc agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag
     1081 agaataatgt cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc
     1141 agaaattggt gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc
     1201 taaagaatgt ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc
     1261 caaaaaggct ccaatggcaa aatatcagaa ggataatctt aaaga
//
LOCUS       DAZKMK010000209         1303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000209 DAZKMK010000000
VERSION     DAZKMK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.217"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            502..1035
                     /locus_tag="ACG8V1_002929"
     CDS             502..1035
                     /locus_tag="ACG8V1_002929"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0330933.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          421 a          239 c          242 g          401 t
ORIGIN      
        1 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
       61 agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct tttgaacacc
      121 gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa caaatggcaa
      181 aatagttcct catattttgc tattttttga gctaataccg agggtcagct gattgaacac
      241 cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa atttttttga
      301 aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca tgatccccat
      361 ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa atattacata
      421 aaaaattcta tgttttttgt taaatttatt tgcttttttc aaaagtatgt tacagtaatt
      481 tcccaaggag gttttacaga aatgaatttt gttgccatgg attttgaaac tgccaatcat
      541 cagccttata gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat
      601 gaattctata cattgataca gcctgagact ccttttttct ggcgaaatgt ccaaattcac
      661 ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc
      721 caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt tgatacaaag
      781 gtcttagcgg gatgcttaga ttattatcat cttgaacagc ctaattatct ttctttatgt
      841 acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa atcatcgatt gaacactgtg
      901 tgcgaaaatt tgaacatcca attagatcat caccatgatg cattggaaga tagtcgtgca
      961 tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga cagatccatt gaaaaaactt
     1021 gtgactataa agtaaaagaa tacttatgaa acacacaaag aggtcgtgag cctgccgttt
     1081 cactccgagt cacaaggagc aatttcaaaa aatagcttct catgtttatt aaatattgtg
     1141 actggtgacc gaggagctgg caggagaaca ccgtttattg gagaggttgt gaaaaagctg
     1201 ttctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt ttcatttggt
     1261 tggacttatt accgcagatg caagcttttg gacaccgttt acc
//
LOCUS       DAZKMK010000210         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000210 DAZKMK010000000
VERSION     DAZKMK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.218"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG8V1_002930"
     CDS             <1..1244
                     /locus_tag="ACG8V1_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0330934.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKMK010000211         1287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000211 DAZKMK010000000
VERSION     DAZKMK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.219"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..570
                     /locus_tag="ACG8V1_002931"
     CDS             97..570
                     /locus_tag="ACG8V1_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0330935.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            579..806
                     /locus_tag="ACG8V1_002932"
     CDS             579..806
                     /locus_tag="ACG8V1_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330936.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          411 a          200 c          213 g          463 t
ORIGIN      
        1 aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata attattctgc
       61 tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac attgctatta
      121 aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact tttttatact
      181 cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt atttgtttct
      241 gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt gaaactattg
      301 aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact aaaaaaaatc
      361 gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa atttatgaaa
      421 gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt cgtattcatt
      481 ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta
      541 cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc gttaatttag
      601 atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta
      661 cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc
      721 tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc tataaaaatg
      781 actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat aaaaaataat
      841 ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc agatttttta
      901 tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata caaagggggc
      961 cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt tacgttgtag
     1021 ttggatctgc ttttagtata atactttatt cattccacca gactgatagc agtttgagca
     1081 gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag tgcttaaaac
     1141 ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa gctcccatat
     1201 atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac tatgtagttt
     1261 tgataaaatg atttgaataa cagtgat
//
LOCUS       DAZKMK010000212         1275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000212 DAZKMK010000000
VERSION     DAZKMK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.220"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACG8V1_002933"
     CDS             complement(31..1203)
                     /locus_tag="ACG8V1_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0330937.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DAZKMK010000213         1260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000213 DAZKMK010000000
VERSION     DAZKMK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.221"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..224
                     /locus_tag="ACG8V1_002934"
     CDS             <1..224
                     /locus_tag="ACG8V1_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0330938.1"
                     /translation="VFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEE
                     DFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            229..1176
                     /locus_tag="ACG8V1_002935"
     CDS             229..1176
                     /locus_tag="ACG8V1_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0330939.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          412 a          179 c          262 g          407 t
ORIGIN      
        1 aagtttttga aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta
       61 attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta attttagata
      121 ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt ttagaagatt
      181 cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat gagctgttat
      241 cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt aggagaagaa
      301 catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct tgatatgacg
      361 aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga agagatcatg
      421 aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt ggcagattca
      481 gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat gattttagaa
      541 ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt
      601 tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc attgacgcct
      661 tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca tttatatgat
      721 gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa tccgaattct
      781 ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat cgtaggagaa
      841 gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt ttatatagat
      901 gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg gaaacaattc
      961 aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat caatgaaatt
     1021 ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat ccatgactct
     1081 ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg tattttaagt
     1141 ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata agattagagg
     1201 gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa atctgggata
//
LOCUS       DAZKMK010000214         1246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000214 DAZKMK010000000
VERSION     DAZKMK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.222"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8V1_002936"
     CDS             200..742
                     /locus_tag="ACG8V1_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330940.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG8V1_002937"
     CDS             898..>1246
                     /locus_tag="ACG8V1_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0330941.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZKMK010000215         1212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000215 DAZKMK010000000
VERSION     DAZKMK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.223"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(214..1074)
                     /locus_tag="ACG8V1_002938"
     CDS             complement(214..1074)
                     /locus_tag="ACG8V1_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0330942.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          364 a          232 c          175 g          441 t
ORIGIN      
        1 ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa taaatactgc
       61 aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta cgactttatt
      121 ttattactct atcttgctgc catacccgtc tatccctaat catctttggt gaattttttc
      181 aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta tcattttttt
      241 catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa aagctagtgc
      301 atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg cgttcttcgc
      361 taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac aaatatcgat
      421 tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa aaaaaacacc
      481 acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa tcggatacgt
      541 ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact ctttttctct
      601 agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt tggctgtttc
      661 tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat tcgtaaaaat
      721 catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt caattacctt
      781 gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa tcagtgaaaa
      841 ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg aagaaaaaag
      901 ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta acgaagggac
      961 ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt cagacacttc
     1021 tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt tcattatttt
     1081 ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat gttttctttg
     1141 ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct tacggtatct
     1201 atgatttaag ct
//
LOCUS       DAZKMK010000216         1145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000216 DAZKMK010000000
VERSION     DAZKMK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.225"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..263
                     /locus_tag="ACG8V1_002939"
     CDS             3..263
                     /locus_tag="ACG8V1_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330943.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(625..864)
                     /locus_tag="ACG8V1_002940"
     CDS             complement(625..864)
                     /locus_tag="ACG8V1_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330944.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          402 a          163 c          211 g          369 t
ORIGIN      
        1 atatgaataa cgaaactatc attttaggaa aaaaatatat ttgccaacct attggcttga
       61 aacaaaaagt ggttggagaa gtaatcagta aattagaaaa ttgtgttgtg atctgcgtag
      121 acgaatatca tgacgtcgat cgcaatgaaa tattagaaaa atgcggaaaa gtagtggcaa
      181 aatatactca cgtttatgag ttggaaacaa cagaatatct atataactat agcaacaaat
      241 atgaacatac tttagtttca taaagattta aataaagtgc cttcaaaagc gagaagacca
      301 agcttttgaa ggcactttat ttagttggca gtgattgttc atgatttttg atttattcaa
      361 aaggttcgcc tagagaatct gtctctgtaa cgaggcctgg tggagaaact cgataaaaac
      421 ctatactgcc agaactgtcc tattttcgga tagacaaact agtagtttta aaaagatcat
      481 ccgatccaat catttcaaaa aatgcaaaac tgatatcagg atcatttagc tcaggcttgt
      541 tatcgtaaag catttgtaaa ggttgcattt agttaggagt aaacgcgaaa ctatcatcat
      601 tatttgctga agaagatgaa gaagctaatt cagagtttgt ttgaaataaa ggagacgtgc
      661 tgacagtttc tttaccctgt tttttgcaga ggtagaaact tttatatcgg aagtttctac
      721 cggagaattt ttctcatttg tagaaattgt tgaatcgata ctattcgaaa tttgcgtatc
      781 tgaaggtatg ttctttgatg cagaagtccc acaagcagta agtaacaata aatttgataa
      841 actgataaac agaattcttt tcattatgta cacactcctt atagtatcgt ttagcataca
      901 cctatgctca gtattaggca aaatcaatgc aaaactatca agtcttttat agtatctatt
      961 agtcttagta atacatatat ggagagggtg atatcccagt tcaaaccaga tacattggtt
     1021 accatctgta ttaacgatta tgcctctaaa cgcttttttt atgaaaaaat ttataagtga
     1081 aaacgtaggt atgatattat ctagaggtaa tttttttggg tatttaaata gttgctagga
     1141 ggata
//
LOCUS       DAZKMK010000217         1137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000217 DAZKMK010000000
VERSION     DAZKMK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.226"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8V1_002941"
     CDS             <1..355
                     /locus_tag="ACG8V1_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330945.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG8V1_002942"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG8V1_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ACG8V1_002943"
     CDS             985..>1137
                     /locus_tag="ACG8V1_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0330946.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAZKMK010000218         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000218 DAZKMK010000000
VERSION     DAZKMK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.227"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8V1_002944"
     rRNA            <1..>1073
                     /locus_tag="ACG8V1_002944"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKMK010000219         1067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000219 DAZKMK010000000
VERSION     DAZKMK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.229"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8V1_002945"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8V1_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0330947.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DAZKMK010000220         1051 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000220 DAZKMK010000000
VERSION     DAZKMK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.230"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1051
                     /gene="rpsA"
                     /locus_tag="ACG8V1_002946"
     CDS             <1..>1051
                     /gene="rpsA"
                     /locus_tag="ACG8V1_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0330948.1"
                     /translation="STLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRLDA
                     KKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYKGK
                     TLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFGAF
                     VDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQPGP
                     WSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLHEG
                     DEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILGDA
                     LKEEVEEKATDDSEK"
BASE COUNT          359 a          175 c          239 g          278 t
ORIGIN      
        1 tatcaacttt gccggtagaa gatatcagcg aagtagtaaa agtcggtgat gtattagatt
       61 tagtcgttat cagtacgatc ggcaaagata aagaaaacgg cagctatcta ctttcaaaac
      121 gccgtttaga tgctaaaaaa gtttgggaag atatcgaaag agatttccaa gagggcaagg
      181 tcatcgaagc acctgttaca aatgttgtca aaggcggttt agtcgttgac gtcggtgtac
      241 gtggttttgt ccctgcatca atggtggaag atcattttgt tgcagacttt tcagactata
      301 aaggcaaaac attgagcttt aaaatcatcg aaatcgaacc atcagaaaat cgtttgatct
      361 tatcgcataa agcggtagtg gaaaacgaaa gagaagaaaa gaaaaaagaa attctttcaa
      421 ctatccatga gggagatgtt ctcagtggac gtgttgcacg tcttactgat tttggcgcat
      481 ttgtcgacct aggcggaatt gacggtctag tccatgtctc agagatttca catagtcatg
      541 tagcaaaacc aagtgatgta ttggctgtca atgatgatgt aaatgtcaaa gtgttgtcta
      601 tcaatcctga acaagaacgt gtttccttgt caatcaaaga cacacagcct ggaccttggt
      661 cagaaatcga tacaaaagct ccagttggca cagtattaga cggaacagtt aaacgtttga
      721 caagcttcgg tgcctttgtt gaagtgttcc ctggagtaga agggctagtc catatctctc
      781 aaatttcaca taaacatatt gcgacaccac atgaagtgct tcatgaaggt gacgaagtga
      841 aagtgaaagt attagagatc catccagaag aacatcgtat tgcattgagt atcaaagctt
      901 tagaagaaaa accagcttca gaagaagaac caaaaaatgt tgagtcttat gaattaccag
      961 aagaaaatac tggatttact atgggagaca ttttaggtga tgcgttgaaa gaagaagtag
     1021 aagaaaaagc aaccgatgat tctgaaaaat a
//
LOCUS       DAZKMK010000221          970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000221 DAZKMK010000000
VERSION     DAZKMK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.231"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>970
                     /locus_tag="ACG8V1_002947"
     CDS             <1..>970
                     /locus_tag="ACG8V1_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0330949.1"
                     /translation="KHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRF
                     YRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYF
                     SRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKET
                     TVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLA
                     ACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQN
                     ALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHSKTFL"
BASE COUNT          302 a          196 c          184 g          288 t
ORIGIN      
        1 aaacactgtt tcattagtca aaaagttcgt tggtctgtca ttgctcgctt aaaagagaat
       61 acttccatga cggaaatcgc tcgtcagaaa aatatttcca cttcttcggt ctatcgtgtc
      121 atgaagcgat tctaccgccc attgaatcct ttcaaacaga ctttgccaaa ggtcttatgc
      181 tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa tgagttttat catgatggac
      241 ggacaaagcc atgcattatt ggatattgtc gaaaatcgac gcttgcctta tttagaacgc
      301 tatttttctc ggttttcatt agcgactcgt gaagccgttc aattgattgt tattgatatg
      361 tatgcgcctt atgtttcgtt ggtgaaaaag cttttcccga atgctcagtt gatcatcgat
      421 cgtttccaca tcgtccaaca tattggcaga actttcttga atcatcgagt gaaagaaacc
      481 acggtacgac ttaaaggaaa ttcccactct caacgagggt tagggaaaaa attgaagcgc
      541 tattggaaac tcctacaaaa agaagaagca aagttaaact atgaaaaacg ggtttggcgt
      601 gccagtttta aagattattt gactgaaagt gagattgccg atcgactgtt agctgcttgt
      661 cccaacttac gccaaggcta tcagttatac caagacattc tttatgcggt aaagaaaaga
      721 gatcagtcac tatttgagga ttgtttaact agagacatta gcggtctgcc agaaacctat
      781 acaaccactt tacgaacgtt taaaaagttt ctgccacaga tccaaaatgc gcttcactac
      841 agctattcaa acggaccatt ggaatgctta aacaaccaca tcaaagtatt aaaacgcaat
      901 gcgtacggct ttcgcaactt ttacaatttc aaactacgaa ttatgattcg tcatagcaaa
      961 acatttttaa
//
LOCUS       DAZKMK010000222          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000222 DAZKMK010000000
VERSION     DAZKMK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.232"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8V1_002948"
     CDS             <1..459
                     /locus_tag="ACG8V1_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330950.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8V1_002949"
     CDS             473..904
                     /locus_tag="ACG8V1_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330951.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DAZKMK010000223          954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000223 DAZKMK010000000
VERSION     DAZKMK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.233"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>954)
                     /locus_tag="ACG8V1_002950"
     CDS             complement(37..>954)
                     /locus_tag="ACG8V1_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330952.1"
                     /translation="KMLKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSL
                     FFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIES
                     FENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIE
                     YINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKK
                     NLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKIL
                     LTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          308 a          162 c          145 g          339 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca tttt
//
LOCUS       DAZKMK010000224          923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000224 DAZKMK010000000
VERSION     DAZKMK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.234"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..781
                     /locus_tag="ACG8V1_002951"
     CDS             89..781
                     /locus_tag="ACG8V1_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0330953.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          400 a          146 c          155 g          222 t
ORIGIN      
        1 ttacatatgt aatttaaaaa cgcttgacat tgtgtttgag atcacttact tttagcatgt
       61 aaaagtattg gattaaggag ctatgcaaat gaaaaagaaa tttattactg gtgggattgt
      121 cgttgcattt ctatgtctaa ttggctacac aggcacgcgg tacgcaaatg aaattatttt
      181 gtggggtgga gagacagata ttgatacaat caatgagaat ctgacacagc ttgatcatac
      241 attgacagat aaagagcaag cgattacaga tcttaccgct cgactagcac aaaaagaaca
      301 gacattgcaa gattcaaagg cagatgtcca agaataccaa acaaaagtaa atgaattaga
      361 aaaccaaaaa aatcaatcgg cagcagaaaa acaaaactta gaaagtcaat tagtcagcaa
      421 ggatgcacaa ctacaaagca aacaaaatga aatcaatgtc aaactagatg agatcaacca
      481 aaaaaatgat gaaatcaatc atctgaatgc agattggaca gcaagactac aagaagcaca
      541 agcaaaactt tcagaattac aaaatactgt aaatggtttg aatcaacaaa tcgcaacact
      601 gacacaagaa aaaacaaatg tagaaactca gttaacagat acacaaaaga aattagacga
      661 agcaactgct gaaaactcaa gcttgaaaca atatattcaa aagttagaaa aagcaaaaga
      721 tgaagtggaa gatacagctg ataaatctca gcaaattgtt gaagaacatt tgcgtaaata
      781 atctttctta acaagtaaac ctaaattagt aaagaagcaa cagtaatcta tactaagtag
      841 ttcagttttg gagtacaaaa tgacaaaatg tacttcaaaa tggactactt ttttcatttt
      901 aacttacata ccaaattaca tac
//
LOCUS       DAZKMK010000225          899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000225 DAZKMK010000000
VERSION     DAZKMK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.235"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..879
                     /locus_tag="ACG8V1_002952"
     CDS             256..879
                     /locus_tag="ACG8V1_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0330954.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaagaa
//
LOCUS       DAZKMK010000226          890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000226 DAZKMK010000000
VERSION     DAZKMK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.236"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8V1_002953"
     CDS             <1..888
                     /locus_tag="ACG8V1_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0330955.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAZKMK010000227          869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000227 DAZKMK010000000
VERSION     DAZKMK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.237"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>869
                     /gene="bsh"
                     /locus_tag="ACG8V1_002954"
     CDS             4..>869
                     /gene="bsh"
                     /locus_tag="ACG8V1_002954"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0330956.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRT"
BASE COUNT          292 a          118 c          159 g          300 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacata
//
LOCUS       DAZKMK010000228          862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000228 DAZKMK010000000
VERSION     DAZKMK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.238"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>862
                     /locus_tag="ACG8V1_002955"
     CDS             <1..>862
                     /locus_tag="ACG8V1_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0330957.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSK"
BASE COUNT          292 a          148 c          176 g          246 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa ga
//
LOCUS       DAZKMK010000229          859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000229 DAZKMK010000000
VERSION     DAZKMK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.239"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..815
                     /locus_tag="ACG8V1_002956"
     CDS             <1..815
                     /locus_tag="ACG8V1_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0330958.1"
                     /translation="IQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLR
                     TIDDLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYI
                     NECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVN
                     NYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISN
                     MDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          386 a           58 c          121 g          294 t
ORIGIN      
        1 atatacaaga aataaaaatt gttactgata aaatgatgaa acttgataaa aaaatgagtt
       61 taaaagttag ctctaatgat gaagttggag aattaaaaca acaaattaat gatttgtatt
      121 ctactttatt aagaacaatt gacgatttgg aatttaaaaa taaagaaatt ttaaaattag
      181 aaaagttaaa atatgacttt tttaaaggtg catcccatga acttaaaacc cctcttgcta
      241 gtcttaaaat aatactggaa aacatgaaat acaatattgg aaaatataaa gaaagagata
      301 tttatattaa tgaatgtatt gaaatcgttg atagtttaac aaaaaatatt tctcaaatat
      361 tatcagttca ttctatagaa aatttgaata atgatgaaga atatttgaaa ataaatgata
      421 cattagaaga catattaaaa aagtatgaaa tattagcaaa tcaaaagaaa ataactgtca
      481 ataattatat attagacgag aatgtttata taggaaaaac agctttaaag attattctat
      541 ctaatttgat tagtaatgcg gtaaaatata ctgatgtaaa tggggtaatt aatattggga
      601 tagttaatga ttggttatat atagagaatt catacggtaa caataaaatt tcgaatatgg
      661 ataaaatatt tgatgttaaa tttgatttaa ataaggaaaa tagtaatgga ttaggtttat
      721 atattgtaag taatattctg aataattaca atatagaata taaagtattg caggatgaag
      781 aattttttat ctttaaaata aaaatatttt cttaattaag atataatttt gcaatagtta
      841 ttgaaaaata gagacttat
//
LOCUS       DAZKMK010000230          816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000230 DAZKMK010000000
VERSION     DAZKMK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.240"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>816)
                     /locus_tag="ACG8V1_002957"
     rRNA            complement(<1..>816)
                     /locus_tag="ACG8V1_002957"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          165 a          259 c          181 g          211 t
ORIGIN      
        1 ctctagagtg gtcaaaggat gtcaagacct ggtaaggttc ttcgcgttgc ttcgaattaa
       61 accacatgct ccaccgcttg tgcgggcccc cgtcaattcc tttgagtttc aaccttgcgg
      121 tcgtactccc caggcggagt gcttaatgcg ttagctgcag cactgaaggg cggaaaccct
      181 ccaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat cctgtttgct
      241 ccccacgctt tcgagcctca gcgtcagtta cagaccagag agccgccttc gccactggtg
      301 ttcctccata tatctacgca tttcaccgct acacatggaa ttccactctc ctcttctgca
      361 ctcaagtctc ccagtttcca atgaccctcc ccggttgagc cgggggcttt cacatcagac
      421 ttaagaaacc gcctgcgctc gctttacgcc caataaatcc ggacaacgct tgccacctac
      481 gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttagat accgtcaagg
      541 gatgaacagt tactctcatc cttgttcttc tctaacaaca gagttttacg atccgaaaac
      601 cttcttcact cacgcggcgt tgctcggtca gactttcgtc cattgccgaa gattccctac
      661 tgctgcctcc cgtaggagtt tgggccgtgt ctcagtccca atgtggccga tcaccctctc
      721 aggtcggcta tgcatcgtgg ccttggtgag ccgttacctc accaactagc taatgcaccg
      781 cgggtccatc catcagcgac acccgaaagc gccttt
//
LOCUS       DAZKMK010000231          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000231 DAZKMK010000000
VERSION     DAZKMK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.241"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8V1_002958"
     CDS             <1..672
                     /locus_tag="ACG8V1_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0330959.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKMK010000232          809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000232 DAZKMK010000000
VERSION     DAZKMK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.242"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8V1_002959"
     CDS             134..307
                     /locus_tag="ACG8V1_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330960.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          272 a          131 c          102 g          304 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagct
//
LOCUS       DAZKMK010000233          801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000233 DAZKMK010000000
VERSION     DAZKMK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.243"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..285
                     /locus_tag="ACG8V1_002960"
     CDS             100..285
                     /locus_tag="ACG8V1_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330961.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            298..546
                     /locus_tag="ACG8V1_002961"
     CDS             298..546
                     /locus_tag="ACG8V1_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330962.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            577..747
                     /locus_tag="ACG8V1_002962"
     CDS             577..747
                     /locus_tag="ACG8V1_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330963.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
BASE COUNT          289 a          107 c          173 g          232 t
ORIGIN      
        1 tttaactatg tattcataat agcttaaaag ctaataggtg tcaacgattt agctaatttt
       61 ttagcttaca aattatttgt ttagaaagga gctattttta tgtctgagaa tttagactta
      121 aaaattcgag cggagatgag aaaaagaaga atgactttca aagaactagc tgcgcttatc
      181 ggtatttcag gagcttattt atcagatatt ctaaacggca atcgtgatgg aaagaaagca
      241 caacagcata tcgaaacagt gaaaaaaata ttggacatcc gatagggggg gtaggcgatg
      301 acaaaactaa aaaaacaaga ttttgtaaaa aaatataatt attctccatc tacttatcaa
      361 cgtcgaatgt cggaactaaa aaatacagca attttctcag cggcgtatga acgggtcaca
      421 ggacaagaag tttggatcaa tacagaatta tacgataaat ttttgtcttt caaatcctat
      481 aacaggttac gcacaagaaa ggtaacacct aaagaattta tcgagaagca tttagttgat
      541 ttataaaaaa cagaacattt tgagaggtga aggttaatgg gtaaattcaa cagagcatta
      601 gtattcagcg caccgctaat cgtctatgct ttagggcttt ggggaagtag acaagcgttg
      661 ataggaacga ttgtgtacat ggtttggatt tttatagggc tggatgaagc tgagtacaaa
      721 acaaaaaagc cagtcgggag ggactgacta atgagtaagg acgatattgt atttctgtta
      781 attatgtttt gtgggattgg a
//
LOCUS       DAZKMK010000234          783 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000234 DAZKMK010000000
VERSION     DAZKMK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.244"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..125)
                     /locus_tag="ACG8V1_002963"
     tRNA            complement(53..125)
                     /locus_tag="ACG8V1_002963"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:His,seq:gtg)
     gene            complement(128..201)
                     /locus_tag="ACG8V1_002964"
     tRNA            complement(128..201)
                     /locus_tag="ACG8V1_002964"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Trp,seq:cca)
     gene            complement(208..288)
                     /locus_tag="ACG8V1_002965"
     tRNA            complement(208..288)
                     /locus_tag="ACG8V1_002965"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(252..254),aa:Tyr,seq:gta)
     gene            complement(290..365)
                     /locus_tag="ACG8V1_002966"
     tRNA            complement(290..365)
                     /locus_tag="ACG8V1_002966"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(330..332),aa:Phe,seq:gaa)
     gene            complement(401..473)
                     /locus_tag="ACG8V1_002967"
     tRNA            complement(401..473)
                     /locus_tag="ACG8V1_002967"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(438..440),aa:Asp,seq:gtc)
     gene            complement(483..555)
                     /locus_tag="ACG8V1_002968"
     tRNA            complement(483..555)
                     /locus_tag="ACG8V1_002968"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(520..522),aa:Val,seq:tac)
     gene            complement(571..642)
                     /locus_tag="ACG8V1_002969"
     tRNA            complement(571..642)
                     /locus_tag="ACG8V1_002969"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Glu,seq:ttc)
     gene            complement(657..746)
                     /locus_tag="ACG8V1_002970"
     tRNA            complement(657..746)
                     /locus_tag="ACG8V1_002970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(710..712),aa:Ser,seq:gga)
BASE COUNT          214 a          215 c          182 g          172 t
ORIGIN      
        1 gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaaa agggcgaatg
       61 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
      121 ttcgccatgg caggggcagc aggaattgaa cccacactaa cggttttgga gaccgctgtt
      181 ctacctttaa actatgcccc tataaaatgg aggagagtgg attcgaacca ctgaacccta
      241 aggaacggat ttacagtccg tcgcgtttag ccacttcgct actcctccat ggtggcgcgg
      301 gacagaatcg aactgccgac acacggagct tcaatccgtt gctctaccaa ctgagctacc
      361 gcgccatatt ttaaaattat taattgaata acaagaacga cggttccgac gggaatcgaa
      421 cccgcgatct cctgcgtgac aggcaggcat gttaacccct acaccacgga accagttcat
      481 tatggaggtt aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag
      541 ctgagctaaa cctccaacat tcaattaaaa tgacccgtac gggattcgaa cccgtgttac
      601 cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaat ttgctacgga
      661 gagtaaggga ttcgaaccct tgagacagtt tggcaccatc tacatgattt ccaatcatgc
      721 tccttcggcc tctcggacaa ctctccagag tgaaagaagc tcaatactct ttctcaaaat
      781 gag
//
LOCUS       DAZKMK010000235          774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000235 DAZKMK010000000
VERSION     DAZKMK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.245"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..685
                     /locus_tag="ACG8V1_002971"
     CDS             47..685
                     /locus_tag="ACG8V1_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0330964.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          242 a          125 c          126 g          281 t
ORIGIN      
        1 gtatactgac tctatcttca aaaactgaat agtaggagtt attatcatga ataacatttt
       61 aggaaaaaaa atagaactta aaaaagggat attgggcact attgtcgttg tactcggttt
      121 gttagggttg ctatttgcgc catccattat tagcattttt tctttaacta tcactggatt
      181 tatagcattt acacttgttt ttggtaaaaa agatacgaaa aaaatgttct cgaaacctgt
      241 cgctcctgtc aaaaatatag ccaaatattt ttttatcaat gtagtaatca gtgccgcagt
      301 ctcggtattt ttgcaacaaa ttttgaaatg gggtttaaca ggcaatccaa taaatgaggc
      361 tttcagcccg ttattattta ttattttgcc tatcatgata cttggagaag aattgttctc
      421 agtgtatttt ctcgctattt tttctagtaa gttcagcatt cctgtcgcta gtttcttgtc
      481 agcaatcatc tttggattta tccattacag tacttatgat aatggaaata ttttacacac
      541 tgtagcacac atccttttga tacaaggggt ggcaagattg ttattcaacc aagctgccat
      601 taaaagcaac tcgatcatta caagttgggc agttcatgtg atatttgatt tcacagctat
      661 attgctagtc gttttattct cctaaatttt tatagaaata atacatgctc tattgctaag
      721 aaagcatact ttatttcctt taaacaaaaa gacagatgta tcaatggatt aaaa
//
LOCUS       DAZKMK010000236          756 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000236 DAZKMK010000000
VERSION     DAZKMK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.246"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..>756)
                     /locus_tag="ACG8V1_002972"
     CDS             complement(91..>756)
                     /locus_tag="ACG8V1_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0330965.1"
                     /translation="RRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQ
                     EQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYS
                     LTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVH
                     QYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSG
                     EAA"
BASE COUNT          204 a          178 c          146 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gcgccg
//
LOCUS       DAZKMK010000237          731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000237 DAZKMK010000000
VERSION     DAZKMK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.247"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(333..446)
                     /locus_tag="ACG8V1_002973"
                     /pseudo
     CDS             complement(333..446)
                     /locus_tag="ACG8V1_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          268 a          115 c          112 g          236 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa a
//
LOCUS       DAZKMK010000238          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000238 DAZKMK010000000
VERSION     DAZKMK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.248"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG8V1_002974"
     CDS             <1..>725
                     /locus_tag="ACG8V1_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0330966.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKMK010000239          719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000239 DAZKMK010000000
VERSION     DAZKMK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.249"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..>719)
                     /locus_tag="ACG8V1_002975"
     CDS             complement(334..>719)
                     /locus_tag="ACG8V1_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFQ0330967.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          247 a          101 c          125 g          246 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgat
//
LOCUS       DAZKMK010000240          717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000240 DAZKMK010000000
VERSION     DAZKMK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.250"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG8V1_002976"
     CDS             <1..608
                     /locus_tag="ACG8V1_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0330968.1"
                     /translation="IGTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGT
                     GVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRL
                     GKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFA
                     KLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          201 a          142 c          182 g          192 t
ORIGIN      
        1 tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg
       61 cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact
      121 atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt
      181 ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg
      241 gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa
      301 aacgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag
      361 cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca
      421 tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg
      481 caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag
      541 cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac
      601 attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
      661 aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DAZKMK010000241          670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000241 DAZKMK010000000
VERSION     DAZKMK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.252"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACG8V1_002977"
     CDS             154..417
                     /locus_tag="ACG8V1_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0330969.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="ACG8V1_002978"
     CDS             417..>670
                     /locus_tag="ACG8V1_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0330970.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DAZKMK010000242          664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000242 DAZKMK010000000
VERSION     DAZKMK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.253"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..345
                     /locus_tag="ACG8V1_002979"
     CDS             133..345
                     /locus_tag="ACG8V1_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330971.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            507..>664
                     /locus_tag="ACG8V1_002980"
     CDS             507..>664
                     /locus_tag="ACG8V1_002980"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0330972.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDP"
BASE COUNT          240 a           98 c          113 g          213 t
ORIGIN      
        1 aaaaacacta tatattccga aaagtgagta tctccattca taaaataatc aacatattta
       61 aaaaatctaa ttagcttttt tagtcaatct aaagttcttt attctatgat ataatgttac
      121 tgtagaaagt ttgtggcggt ttggcaaatc caattgaagg agcgtggtgc ctatgggctt
      181 attagcttat cggacgtact ctgtagaaag gaaatgcctg atttgttctc attggatcaa
      241 atcattgcta ttatcggagc agcagcagta gttattactg ccgtagcgaa agctagcgat
      301 tcgattttag cttggttaaa gttcaaaaaa gaacaaaaaa aatagagccg tctcaacttt
      361 tggcgaggta gacgagctct aaagctttat ttacatgcca tccgctttga acggactaca
      421 ctgggcatgt gacagcatgc cctttcattt atattatatc tcattctttt atataatgaa
      481 agtaaataaa tcatggagga taaaatatgg ataaatttaa tagattaaca ttaataaatc
      541 agtttgaaat tttaaaagct ttaaatccta acgaagaaaa atactatgct gaaaaaatag
      601 aaatactaac agaaggttac gaatatcact atagtgaaat atttgaaaat atttctgatc
      661 catt
//
LOCUS       DAZKMK010000243          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000243 DAZKMK010000000
VERSION     DAZKMK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.254"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8V1_002981"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8V1_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0330973.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAZKMK010000244          623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000244 DAZKMK010000000
VERSION     DAZKMK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.255"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..576)
                     /locus_tag="ACG8V1_002982"
     CDS             complement(28..576)
                     /locus_tag="ACG8V1_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0330974.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          200 a          140 c           60 g          223 t
ORIGIN      
        1 ctcttgttct aaacttttca taaactttta ctccaatgca cagctagcag cagctgctgc
       61 aattcctgct acaatattta ccttagctaa ctctttagct gcagcttttc ctaattgttt
      121 ttctgcaatt tttaataata aatccgcagc tttttcaaaa ttaaaagctt ttaatgcttc
      181 aataaaatct ccctgcgtac ctatagccca agccactcct cctccaggaa gagaattaat
      241 ggctacacat ccagcatatc gtttccaatc aactttctcc attctaggaa caataatatg
      301 ttcataaaag actcttgctg ctcttccttg ttcgttatct tgactgattc gttcacgaat
      361 agcaccaaaa tcctttatat ggtatacacc atgttcgtct aaaaaaccaa ctgtatcaaa
      421 ataaaaagtt aattcatcta ctaatttatt ctttatttct aaatcaactg tattcacttt
      481 ttctgtggca tacacacttg ttccagtact aacaaaagta cttaaaccta ataagaaaac
      541 acctaaaaca ccaaatattt taattattct tttcataatt tctcctccat aaaaaataag
      601 ttacttttta aatatcttgt tta
//
LOCUS       DAZKMK010000245          608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000245 DAZKMK010000000
VERSION     DAZKMK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.256"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..400
                     /locus_tag="ACG8V1_002983"
     CDS             143..400
                     /locus_tag="ACG8V1_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330975.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          252 a           76 c           83 g          197 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa attagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgacaaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgca
//
LOCUS       DAZKMK010000246          606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000246 DAZKMK010000000
VERSION     DAZKMK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.257"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..579
                     /gene="spxA"
                     /locus_tag="ACG8V1_002984"
     CDS             181..579
                     /gene="spxA"
                     /locus_tag="ACG8V1_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0330976.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          213 a           94 c          118 g          181 t
ORIGIN      
        1 aaaagcgaga atgctggttt tactgtatat taaccttaga taactttcat ttttttaaaa
       61 aaattatgaa aaaaaggtag ctatttaaaa gttgattatg tataatagaa acagatggga
      121 gattattatg atcttctgtt acaaaaaagt gattcttatc acgaaaagga gtggtgcacg
      181 ttgttaacat tatatacttc cccaagttgt acttcctgca gaaaggctcg tgcgtggttg
      241 caagaacatc agattccatt tgttgaacgt aatatttttt cagaaccatt gaacagttct
      301 gaattaaaag cgattttaca aatgacagaa gacggaacag aagaaatcat ctcgacacgt
      361 tccaaagtat tccaaaaact gaacatggat ttagatgatc tgccgttaca agaattattg
      421 gaacttgtgc aaaacaatcc tggattgctt cgccgtccaa tcatgattga tgataaacgt
      481 ctgcaagttg gttttaatga agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa
      541 ttagaattac gtcaagcaca attgatggct ggattataga aggataatac gaaacttgga
      601 aaagac
//
LOCUS       DAZKMK010000247          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000247 DAZKMK010000000
VERSION     DAZKMK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.258"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..580
                     /locus_tag="ACG8V1_002985"
     CDS             <1..580
                     /locus_tag="ACG8V1_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0330977.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DAZKMK010000248          580 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000248 DAZKMK010000000
VERSION     DAZKMK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.259"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..>580)
                     /locus_tag="ACG8V1_002986"
     CDS             complement(35..>580)
                     /locus_tag="ACG8V1_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0330978.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          164 a          110 c          100 g          206 t
ORIGIN      
        1 tccactcctt ttttcatgct ctaaaatgat atttttacac aactgttacg gttgttttac
       61 gaaattggac gggactttgc cagtttaatt tctgttttat ccgttcatta ttgtagtaat
      121 agatatagct atcgatggct gattttaatt cctcgaaaca gttataaact ttcccgtgaa
      181 aaatctcttg ttttaataag ccaaaaaaat tctccataag tgaattatct aagcagttcc
      241 ctttccgaga catgctttga aagattttgt actccttcaa tttgtttcta taagctttca
      301 tttgatacgc ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg
      361 tctggatagc ttcctccaaa ccttccatta ccgctaatgc attgggcttt tcagagatac
      421 gataggataa aatctcacta ttgaacatat ccataaaagg atctagatac aatttctttt
      481 gacggataat gcctttggca tccacttcat ggtatttaaa ttcagttgta tccgtcgtga
      541 ttttctgatg gcagatactg gtgtagaaac gacgatggat
//
LOCUS       DAZKMK010000249          570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000249 DAZKMK010000000
VERSION     DAZKMK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.260"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG8V1_002987"
     CDS             complement(<1..461)
                     /locus_tag="ACG8V1_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0330979.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          172 a          111 c           76 g          211 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt
//
LOCUS       DAZKMK010000250          527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000250 DAZKMK010000000
VERSION     DAZKMK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.261"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..>527
                     /locus_tag="ACG8V1_002988"
     CDS             31..>527
                     /locus_tag="ACG8V1_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0330980.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          193 a           98 c           86 g          150 t
ORIGIN      
        1 caaccaattc acagaaaaga ggacttccct atgaatgatt ttactacaga aattgtgcaa
       61 actctagtca ctaaaggcga tttaaatgaa ttattccgtt cgcacttaga aaaagcgata
      121 aacacactcc tacggactga attaacggct tttttagatt acgaaaaata tgatcgcact
      181 ggttttaatt caggtaattc gagaaacggt tcttactttc gatcaatcaa aaccgaatat
      241 ggtgaattaa cattggaaat acctagagat cgtaatggtg agtttaaaca acaaacttta
      301 ccagcctaca aaagaacaaa cgatacattg gaaaccacta ttatccattt attcgaaaaa
      361 ggtgttacga tgtctgaaat tgctgatttg atcgaaaaaa tgtacggtca tcactatact
      421 ccacaaacca tgtccaacat gactaaagtt ctgactgaag aagtaaatgc ctttaaatcc
      481 agagccttaa atgataagta tgtcgctatt tttatggacg ctactta
//
LOCUS       DAZKMK010000251          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000251 DAZKMK010000000
VERSION     DAZKMK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.262"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACG8V1_002989"
     CDS             <1..354
                     /locus_tag="ACG8V1_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0330981.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAZKMK010000252          523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000252 DAZKMK010000000
VERSION     DAZKMK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.263"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..170)
                     /locus_tag="ACG8V1_002990"
     tRNA            complement(97..170)
                     /locus_tag="ACG8V1_002990"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(134..136),aa:Ile,seq:cat)
     gene            complement(337..>523)
                     /locus_tag="ACG8V1_002991"
     CDS             complement(337..>523)
                     /locus_tag="ACG8V1_002991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330982.1"
                     /translation="SIKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCLNVFLNAKSMQ
                     KILYFSTFLSLTKIKKR"
BASE COUNT          178 a           87 c           99 g          159 t
ORIGIN      
        1 aaagccactc atttgagtgg caatgaagaa aagctttagc ttgtataata ctcttcaaaa
       61 aattctaaca cagaacgatt caattggcta cattaatgta ccctgtagga ctcgaaccta
      121 cgaccggacg gttatgagcc gtctgctctg accaactgag ctaagggtag tggttgttgc
      181 cacataaagc cataaacaat caaccagtag aatgcgtggc aacaaacctg ttatcgcata
      241 tcttggagtg tgactattta tgagtgatag tgaagatatg cgtctccata tatgtatggt
      301 tgagacagat aaacatgtat gaaaaacgtt gttaaatcaa cgttttttta ttttggtcaa
      361 ggagagaaaa gtagagaaat acaaaatttt ttgcattgat tttgcattca aaaaaacatt
      421 caaacattta gaaatcccca atacgtattc aggaaaatct attttaaggt ttacaaaaag
      481 aacttttgtt cgtacactct ttttgaggtg aacttttatg ctg
//
LOCUS       DAZKMK010000253          507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000253 DAZKMK010000000
VERSION     DAZKMK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.264"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..342
                     /locus_tag="ACG8V1_002992"
     CDS             <1..342
                     /locus_tag="ACG8V1_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0330983.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          204 a           68 c           93 g          142 t
ORIGIN      
        1 gataacatac gagataaaaa ggcaagttta aaatcactaa aagatacatg gcttgattta
       61 tcagaagata atccatacag ccaattctta attactgtaa tggctggtgt taaccaatta
      121 gagcgagatc ttattcggat gagacaacgt gaagggattg aattggctaa gaaagaagga
      181 aagtttaaag gtcgattaaa gaagtatcat aaaaatcacg caggaatgaa ttatgcggta
      241 aagctatata aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct
      301 agggcttcat tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta
      361 atgggcaata tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat
      421 gccgaaaagc cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt
      481 atgtaaatgc ttataaattc ggcccta
//
LOCUS       DAZKMK010000254          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000254 DAZKMK010000000
VERSION     DAZKMK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.265"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..165
                     /locus_tag="ACG8V1_002993"
     CDS             43..165
                     /locus_tag="ACG8V1_002993"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330984.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
BASE COUNT          185 a           73 c           87 g          150 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaat
//
LOCUS       DAZKMK010000255          494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000255 DAZKMK010000000
VERSION     DAZKMK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.266"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..323
                     /locus_tag="ACG8V1_002994"
     CDS             9..323
                     /locus_tag="ACG8V1_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330985.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          164 a           58 c           76 g          196 t
ORIGIN      
        1 gatagctttt ggatttttac agtaatttta tcttaataat agcaatatta ttacttctaa
       61 atatttggtt ttttgataag agtagaaatg cagggatagg ttttagaacc aaacgaagta
      121 cgtcttctga aaaaaagtgg gtatattcac agactatttt ttacggaggg gttatttcaa
      181 tcagtttact ttcttcaaca ctttattctt ttaatgttat agacgtttcc atgtcaaatt
      241 ttatatctat tattggaatc cttatttctg caattattac acaactactt cttgtgtttg
      301 aagagaagtc aaaaaataat taaagcaatt atgcctttta gtacaggaat tattacttct
      361 tacaaatgta tagcgaatgt gaagcctgct gttttaattg ttctagaaaa acttttgagt
      421 gaacaattat tttatgttag gatgaattaa tttctctaga gtttataatt aaaaaactta
      481 tttgaaggaa catg
//
LOCUS       DAZKMK010000256          492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000256 DAZKMK010000000
VERSION     DAZKMK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.267"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          162 a           96 c           57 g          177 t
ORIGIN      
        1 atgctaaatc actctacaat aacggcgaaa gcacaaaaaa taaaaaaatt attacgttat
       61 taaaaaaaca gatgaaagat caggttttat tagatctcat tctttcatca cttgaaggga
      121 tttaatgaac tactttttcc tctttgtcgt ttttatatat tcgtattcct tcaaacagta
      181 ggatagcctc tttcaatgct tgatatttga actgtccgtt acttaaaaat ttagatgtgc
      241 cgccctcttc ctagaacata ttctttttta ctttttcatt aattgacaga ccaatcgaac
      301 gaaactgtaa atttcgatag gataatgatt ggtcaaactc attcattttc aagacatctc
      361 gattcatatt caagatagga atctttttat caacatcact ttattcgtat tttcctctac
      421 cataaaagct tcacgctcca caaaattgtt ttatacacta ctattcagcc cattcaacga
      481 ccaatgaatt tt
//
LOCUS       DAZKMK010000257          490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000257 DAZKMK010000000
VERSION     DAZKMK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.268"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..388
                     /locus_tag="ACG8V1_002995"
     CDS             149..388
                     /locus_tag="ACG8V1_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0330986.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          204 a           58 c           94 g          134 t
ORIGIN      
        1 tctttctcaa aataaacata aaagcccttt aaagagggct tttatatatt aatcacaaat
       61 cacttatcac aaatcactta tcacaaatca caagtgattt gtgattgttg atgataaaat
      121 aagaataaga agaaatagaa agaagtgagt gattgtggga aatttaggcg cacaaaaaga
      181 aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg
      241 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt
      301 aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcatta
      361 tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa
      421 gaaggaggga ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt
      481 tttgaaacag
//
LOCUS       DAZKMK010000258          489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000258 DAZKMK010000000
VERSION     DAZKMK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.269"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..319)
                     /locus_tag="ACG8V1_002996"
     CDS             complement(17..319)
                     /locus_tag="ACG8V1_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0330987.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          170 a           83 c           67 g          169 t
ORIGIN      
        1 ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta tacttcaaat
       61 agtcataacg ccgatgatcg ctccgtgcgt ctggcacatc agtcacgata tcaaacaaaa
      121 aatacacatc tttcttcatt ctggtttttg ttgccggaat tttaaagtag ttcttattag
      181 agtaatagag attgattaat aagctatctt cgattgctaa aaagaaaact tctgaatccc
      241 acaccttata aaaatctttg acaaatctat tcgaaggatc aaatttaaac cataattgtg
      301 tttttccttc catcaacata atgttcacct caaaaagagt atacgaacta atgttctttt
      361 tgtaaagtag gaatatcatt gataatatgg atggaacata tatattaaaa agtgttcttt
      421 ttgaatgcta ttttgaatgc cattttcatt catttaacga aaatctgcga aaataaaaaa
      481 tgctcaaac
//
LOCUS       DAZKMK010000259          478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000259 DAZKMK010000000
VERSION     DAZKMK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.270"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>478
                     /locus_tag="ACG8V1_002997"
     CDS             <1..>478
                     /locus_tag="ACG8V1_002997"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0330988.1"
                     /translation="STFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSL
                     RNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLK
                     LVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTE"
BASE COUNT          189 a           56 c           91 g          142 t
ORIGIN      
        1 tgagtacttt tgttgtggga aacattggac gattagagaa acaaaaaaat caatcttatt
       61 tgttagagat ttttttagaa atactaaaaa tgaattctga ttcttacttg attttagtag
      121 gtgaagggtc acttagaaat aaactgaaag ccaaagcagt agaattggat atagagaaaa
      181 gagtaaaatt tttaggagaa agaaacgata ttcccgagtt attatctgtg atggatgttt
      241 ttgtaatgcc ttctctttat gaaggtctgc caatggttgg attagaagca caagcagcaa
      301 aattaaaatt agtcttagca gatactattt ccaaagatac aaagttaaca aataatgtgg
      361 attttgtatc attagatgaa acaccacaag aatgggcaaa aaaagtaata aaagagccgt
      421 atgctaataa aataacacaa gaactaaaga gttatgatgt ttcgtacact actgaaca
//
LOCUS       DAZKMK010000260          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000260 DAZKMK010000000
VERSION     DAZKMK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.271"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG8V1_002998"
     CDS             <1..>468
                     /locus_tag="ACG8V1_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0330989.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKMK010000261          462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000261 DAZKMK010000000
VERSION     DAZKMK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.272"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG8V1_002999"
     CDS             complement(<1..430)
                     /locus_tag="ACG8V1_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0330990.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAZKMK010000262          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000262 DAZKMK010000000
VERSION     DAZKMK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.273"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG8V1_003000"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG8V1_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKMK010000263          420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000263 DAZKMK010000000
VERSION     DAZKMK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.274"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..403
                     /locus_tag="ACG8V1_003001"
     CDS             74..403
                     /locus_tag="ACG8V1_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0330991.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DAZKMK010000264          419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000264 DAZKMK010000000
VERSION     DAZKMK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.275"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..330
                     /locus_tag="ACG8V1_003002"
     CDS             34..330
                     /locus_tag="ACG8V1_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0330992.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          164 a           60 c           76 g          119 t
ORIGIN      
        1 tatcaaataa aagacttctt tggaggaatc actatgaagg aatatataaa aaacatctat
       61 tttatcgaag aaacacaaaa tatcgagggt tcttatattg aagtaaaaac actctttgtc
      121 aatgaagata aaaccaaagc attggatatt tataaaaaat tagcttcaaa aaaaacgaat
      181 agttttggat tgatattaag cgagtataaa atcaaagcag aagagagtta tttctatcaa
      241 ttactaaaac ggtggagtaa gcttcccgcc gatttttatc gaaaaatgca gatcatcaac
      301 tatcaaccgc ttgctgagac acatgcctaa atagaggttg tgaaatcagt gctttgagct
      361 aataccgaag gtcagctgat tgaacattgt ttagaagaga ttgtgacaac tgcgatttg
//
LOCUS       DAZKMK010000265          413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000265 DAZKMK010000000
VERSION     DAZKMK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.276"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG8V1_003003"
     CDS             <1..390
                     /locus_tag="ACG8V1_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0330993.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caa
//
LOCUS       DAZKMK010000266          391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000266 DAZKMK010000000
VERSION     DAZKMK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.277"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>391
                     /locus_tag="ACG8V1_003004"
     CDS             82..>391
                     /locus_tag="ACG8V1_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0330994.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     S"
BASE COUNT          137 a           65 c           81 g          108 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt t
//
LOCUS       DAZKMK010000267          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000267 DAZKMK010000000
VERSION     DAZKMK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.279"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8V1_003005"
     CDS             <1..>386
                     /locus_tag="ACG8V1_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0330995.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKMK010000268          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000268 DAZKMK010000000
VERSION     DAZKMK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.280"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8V1_003006"
     CDS             <1..>386
                     /locus_tag="ACG8V1_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0330996.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKMK010000269          384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000269 DAZKMK010000000
VERSION     DAZKMK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.281"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          152 a           51 c           51 g          130 t
ORIGIN      
        1 ttcaaatata aaagagttta aagcgtaata cacttatggg gaagtggttt ggggtgcgct
       61 ttaaactctt ctttattatt atctcacagc atgcttaaaa ggtctatgga ttaagttatg
      121 taaaaaagtg cctctttaaa cgtcctaaaa tccaaacaag gatagcataa tatacactat
      181 cgttgatcca gaaaacaggt aaataaattt tgcaaaagca ataagttaaa aacattacaa
      241 aaaagacaag gatatcacat caagaaaaaa tgttttgaaa atatctttgc ctttttgtat
      301 tatatctata ttaaataatc aatcacaaca cattaactaa tttatatatt taatttgttg
      361 tttttctata attggataac taat
//
LOCUS       DAZKMK010000270          383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000270 DAZKMK010000000
VERSION     DAZKMK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.282"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..>383
                     /locus_tag="ACG8V1_003007"
     CDS             141..>383
                     /locus_tag="ACG8V1_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0330997.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT          139 a           62 c           73 g          109 t
ORIGIN      
        1 aattttaaaa aatgataaaa attcaaaaag aaaaaatagt agttatttac ttgtcatgtt
       61 actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga tttcattcta
      121 aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc acaaaatttg
      181 tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt ccgacaacga
      241 cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa gcagatttag
      301 cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc gccacttatg
      361 ggtacattac ctcgaccccg aaa
//
LOCUS       DAZKMK010000271          369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000271 DAZKMK010000000
VERSION     DAZKMK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.283"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..>369
                     /locus_tag="ACG8V1_003008"
     CDS             98..>369
                     /locus_tag="ACG8V1_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0330998.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          139 a           52 c           70 g          108 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttt
//
LOCUS       DAZKMK010000272          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000272 DAZKMK010000000
VERSION     DAZKMK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.285"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG8V1_003009"
     CDS             <1..>344
                     /locus_tag="ACG8V1_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0330999.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKMK010000273          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000273 DAZKMK010000000
VERSION     DAZKMK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.286"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACG8V1_003010"
     CDS             <1..>341
                     /locus_tag="ACG8V1_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0331000.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKMK010000274          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000274 DAZKMK010000000
VERSION     DAZKMK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.287"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..318
                     /locus_tag="ACG8V1_003011"
     CDS             <1..318
                     /locus_tag="ACG8V1_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0331001.1"
                     /translation="NRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMG
                     SIHKSSLLSPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLK
                     DSF"
BASE COUNT          120 a           57 c           49 g          107 t
ORIGIN      
        1 aatcgaatga ttcttactgg cgtaattgca aacccatcaa aaatgccaaa ctcaattatc
       61 gaatgtacgt atctttacaa cggtaagaaa attaattgtt cacattatca cgccaatggt
      121 tttgatatgg gaagcattca taaaagtagc cttttatctc ctataccgtt ggcacaagca
      181 atctctcctg gctactcagt cgctttttca gaagttttag atatgaaaga tatattacct
      241 ggtgaaaaac ttgtaatggt cataagaaca gctaattatc aaaaaaaatt taagttaaag
      301 ctaaaagact cattttaatt gaaaaatttt tct
//
LOCUS       DAZKMK010000275          329 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000275 DAZKMK010000000
VERSION     DAZKMK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.denovo.288"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          108 a           58 c           38 g          125 t
ORIGIN      
        1 tagaagcatg taattttttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat
       61 catcacttac ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca ttgctatcta
      121 gtgtaccttc atttctttgt gcttaaattc aaagataatt ttctcaataa agatttttga
      181 ttccagtaag attcctttac tccttttaca acatagcgtt gtcctttgtt ttgaaacatc
      241 ttcttcccct ctttgccatt aaaaatagcc caattctcta taaagaagat taagcttcat
      301 tattataata taaagtttaa atttggttt
//
LOCUS       DAZKMK010000276        15270 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.guided.064, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000276 DAZKMK010000000
VERSION     DAZKMK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.guided.064"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2720)
                     /gene="uvrA"
                     /locus_tag="ACG8V1_003012"
     CDS             complement(<1..2720)
                     /gene="uvrA"
                     /locus_tag="ACG8V1_003012"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0331002.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGG"
     gene            complement(2730..4724)
                     /gene="uvrB"
                     /locus_tag="ACG8V1_003013"
     CDS             complement(2730..4724)
                     /gene="uvrB"
                     /locus_tag="ACG8V1_003013"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0331003.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            4983..6485
                     /locus_tag="ACG8V1_003014"
     CDS             4983..6485
                     /locus_tag="ACG8V1_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0331004.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            6487..7224
                     /locus_tag="ACG8V1_003015"
     CDS             6487..7224
                     /locus_tag="ACG8V1_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0331005.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7395..8588)
                     /locus_tag="ACG8V1_003016"
     CDS             complement(7395..8588)
                     /locus_tag="ACG8V1_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0331006.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(8667..8873)
                     /locus_tag="ACG8V1_003017"
     CDS             complement(8667..8873)
                     /locus_tag="ACG8V1_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0331007.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(8866..10008)
                     /locus_tag="ACG8V1_003018"
     CDS             complement(8866..10008)
                     /locus_tag="ACG8V1_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0331008.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            10520..11155
                     /locus_tag="ACG8V1_003019"
     CDS             10520..11155
                     /locus_tag="ACG8V1_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0331009.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            11309..11386
                     /locus_tag="ACG8V1_003020"
                     /pseudo
     CDS             11309..11386
                     /locus_tag="ACG8V1_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            11700..11747
                     /locus_tag="ACG8V1_003021"
                     /pseudo
     CDS             11700..11747
                     /locus_tag="ACG8V1_003021"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11763..13682
                     /gene="tet(M)"
                     /locus_tag="ACG8V1_003022"
     CDS             11763..13682
                     /gene="tet(M)"
                     /locus_tag="ACG8V1_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0331010.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            13801..13968
                     /locus_tag="ACG8V1_003023"
     CDS             13801..13968
                     /locus_tag="ACG8V1_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0331011.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14028..14381)
                     /locus_tag="ACG8V1_003024"
     CDS             complement(14028..14381)
                     /locus_tag="ACG8V1_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0331012.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            14885..>15270
                     /locus_tag="ACG8V1_003025"
     CDS             14885..>15270
                     /locus_tag="ACG8V1_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0331013.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGL"
BASE COUNT         4519 a         2999 c         2888 g         4864 t
ORIGIN      
        1 tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac atctagattg
       61 tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac atgcaacaag
      121 cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata gaaattcttt
      181 ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc accaccggac
      241 aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac aatcgtttgc
      301 agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcctc tactgtcatt
      361 tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc agaattatat
      421 cgtttaccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg catttcgatc
      481 ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt gaaactaaaa
      541 cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa cccacggata
      601 tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc gattgggctt
      661 tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt gaatttacct
      721 ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat actattgacc
      781 aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc taatggaaat
      841 tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat tgctttccca
      901 ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag atatttccct
      961 gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac gatttgtccg
     1021 ccttgttctc cggccccagg tccgacgtca atcaaataat ctgctgccca catcgtatct
     1081 tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt taatgagcca
     1141 atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa aatatacaaa
     1201 actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc ttctccccca
     1261 gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt ttccaagaat
     1321 gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc ttgttcagat
     1381 aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt tgtttgcccg
     1441 atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata acctttacat
     1501 gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa atcactgttc
     1561 gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt ttctacatcg
     1621 cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt cccattcaag
     1681 atgatctcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc gataccgaaa
     1741 ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg attccatgga
     1801 acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc ttgatcgact
     1861 tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc attgaatgag
     1921 aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc atagtgttcg
     1981 ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc tgctaatcgc
     2041 agggccgcct ctaaagaatc aaataggcga gaacgaatcc cttctttcac aatgatccgg
     2101 tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac ttcacttaaa
     2161 tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat tcgttcgaat
     2221 atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg gatctttgtg
     2281 cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact ggtaatctcg
     2341 atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg taaatagtca
     2401 ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt tttctgatca
     2461 atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc catttgtcct
     2521 aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc ttcagcgtac
     2581 agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac gaccacgaat
     2641 ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc accatgaatc
     2701 gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc taaaatcata
     2761 tctcgcacat tcgcggctgt ttcaaaatcc aaagcttttg ccgcatcttt catttctttt
     2821 tctaatttca tcaataacgt gtctttttct tccttcgtca tttcttcgta cgagacttgt
     2881 accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc tcggatttct
     2941 ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg aatcgtacga
     3001 cgtcttgacg tttcatccat cgctagacgc atagaatctg ttactttgtc tgcgtacatg
     3061 attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa ggaacgttca
     3121 ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac ttcaggtacg
     3181 tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc taatcgaaga
     3241 tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata tttgactttg
     3301 atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt caatgtcgtt
     3361 acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag atcatcgatt
     3421 tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg acggatgatt
     3481 tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc agagacataa
     3541 atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt gtctaaagca
     3601 gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc accattatac
     3661 attcctctga tttgaggcat ggttacgtgc gattcgtccg ccacgatcaa aaaatcttca
     3721 ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc catatgccga
     3781 gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat atcgtaattg
     3841 gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa gacagttaaa
     3901 cgctgttcta attcctcttt gatattcgct acggcatgct ccatatgttc atcattcgtc
     3961 acaaagtgag tcgccgggaa gattgctacg tgttccgttt ctcctaaaac ttcacctgtc
     4021 agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac acgtaaggca
     4081 cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg aaaacgaccg
     4141 cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg aatcaactga
     4201 ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta ctcaaaaggc
     4261 gagcctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt tctttctaat
     4321 aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact tgaatctttt
     4381 tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc ataataagaa
     4441 acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta cagctgacca
     4501 gccaatgttt tgttatgggc aataatcagt gttggtttat tcactttctc aataagattg
     4561 gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc ttttttcccg
     4621 ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc cggttggtat
     4681 ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg tcctcctttt
     4741 cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag gcattcctta
     4801 ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat tatacatgtt
     4861 atgttttata acataaaatg ttagaaactc gcattttttt cattttttcg agcttacagt
     4921 cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt aggaggttga
     4981 ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga cttgtgacac
     5041 ttgcgtttgg tcatgttgca caagctgaca acactacaac ggcagagtca agcacaagcg
     5101 agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt gactctgctt
     5161 ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat gtaaaattga
     5221 tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt ggtttctctg
     5281 ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga atgactgtaa
     5341 cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc gggatccaat
     5401 tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa ggaaaaacag
     5461 tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa tctgaatatg
     5521 gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta aaagttggcg
     5581 caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa caaggacaag
     5641 aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc gttaaaaaag
     5701 gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta aaaagaacag
     5761 gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact tccagtgagt
     5821 cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat caagcattgt
     5881 tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg gctatagtaa
     5941 tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc acaattgcta
     6001 gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc tttattttct
     6061 ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt gttatcacct
     6121 tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc aacgccgttc
     6181 ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg acaatgcaaa
     6241 aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat cagttcgtta
     6301 tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg ctgcaaactg
     6361 gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc attgcgatca
     6421 tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa aagaaggtga
     6481 agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg cgataacaca
     6541 gtattaaacg acatcaatgt atccatcaac gaaggagatg tcgtctgtat cattggccct
     6601 tcaggttcag gaaaaagtac attcttgcgt tgcttgaatc gtttagaaga accttctgac
     6661 ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat caacttggtg
     6721 agacaacata tcggaatggt attccaacac tttaacctat tccctcatct ttctattttg
     6781 gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga agcagagaaa
     6841 aaagcaatca aattactgca gacagttggt ctagaagata aaaaagatat gtatcctgaa
     6901 atgctttctg gtggacaaaa acagcgtgtg gctattgctc gtgctctagc gatgaaccct
     6961 gacatcatgc tttttgatga gcctacttct gcactcgatc ctgaaatggt cggtgatgtc
     7021 ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt tacgcacgaa
     7081 atggggttcg ctaaagaagt ggccaatcgc gtcatgttca ttgacgacgg aaacttttta
     7141 gaagatggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac aaaagatttc
     7201 ttgaataaag tattaaatat ttaaaataaa ttatatatgt ctttttgact atctttttat
     7261 atcttcttaa atgtccgcaa agccttattt tataggctct acgggcattt tgcttttgtg
     7321 gtaaacctca catatctagg tctatcttct tatattttca ctatcaagcg tggttaaaat
     7381 cgtggtaaat acttctatgc aattagacgc tgaacctctg attttgcggt atctatggac
     7441 gcatgagcat accagttcat tgtgatactg atatttgaat gccccatgat atactgtaaa
     7501 tcttttgggt tcatgttctt gcttgccagt cttgtgcaga atgtatggcg tagcgtatgc
     7561 ggtgtgatat gtggcaatgg attgtccttg tggtgcttgt tgtatttctt taccatacgg
     7621 ataaataagg cgttataatc aatcgcaact ttgggcttgc ccttatgatt gacaaatagg
     7681 aagttgcccc gtccgtctat cacaaatggt tctgcctttg gacgtttttt cataacctgt
     7741 tgaaatgcct gtatcgtttc tttgcttaat ggtacttgcc ttattccgct ttttgtttta
     7801 ggcgtttcaa tataatagcc ctgttccttg ctctttagta actggtggtc gataatcaca
     7861 acttcattct tgaaatcaat gtcggctact gtcagtccgc acagttccga aatacgaagt
     7921 cctgtcttta acagtatcag cacatcatca taatgcttgt gatacacatt gtccgtcttg
     7981 atgaatgaga gtaaggcttg ttcctgttcc tctgtcaatg cgactttctt tttggtatca
     8041 ttttctagga cttcactcaa cttgaaatca aagggatttt tcctcacata atcatcttga
     8101 atggctatgt agaatgacgc ttttaacgag cgtttatggt tgttaatggt attgtaggaa
     8161 aagcctttgt ctttcatgcg taacgcccat tccttagcgt cagagggttt gattgtatca
     8221 atgctcctag cacctaactt gtcctctttc aataaccgca tgagttgttc ccgttgtttc
     8281 tgtgtgcttt tcttcacatt tgccctctgt gcgttctgtt tggcataaag ttggcaaagc
     8341 gtcattttct tgccagtgct gtcgataccg tcctcaatat cccgtcttat ctgctgttcc
     8401 agttctcgaa gtgagggttt ttcccgtttt ccctttggtg tcgggtctgt gggtgtcaat
     8461 ctccaagcat acacatattt tgtgtttcca aatgcgtcta catatttata taagtatttt
     8521 ctgtctgttc gttggctctc tccagtatgc aggatacgac ctttgttatc ccgtcttttt
     8581 tctttcatgg tgtctgctcc tttccttgtt ggaaagagcc ttgatatgac ttgtgttcat
     8641 cataacacat acaaggctca tttgcattag atagcgtcca atttatcaat gacctgttca
     8701 aactgtcggc gtttaatctg tatgcggttg ccattcataa tgagccagcc agcgtccttg
     8761 ttttcctctg ccaatcgtct taacttgttt tctccgatac ggaaatactt tgacgcttct
     8821 tcaatggtaa gggtgtattt ttcccatacg ggaatatcct gtgcattact cataaaacac
     8881 cccctcactt tcgattacgt ctgctatgtc cgtacacatc tgttccacaa gcatttcctg
     8941 cttttcagag aggtgggtac attcatcaat cattttcatg tagtttgagc ctgtcagcct
     9001 gtcgatttcc tctatcatca cgattgacgg catgacctgt ctttccagcc attgcagagt
     9061 gcgttccata ttgacaggta cggggttcat ttcaaaaggt aaactgtcat gggaaatgaa
     9121 tatctcccac agcggatagt cgggaaattg cacaaaatca gtaataaggt aaaacaccag
     9181 cccatgaggg ttatatgtca ataacaattc ctcaactgcc tgtactgctt ttgtaccacg
     9241 cagacgaatt tcaaaacgat tgataatatc cgtatgtttc tttttggttg cctgttcctt
     9301 ttgtttttca tacaggcaga agtattttgt gcttgccttt gaaccgatat aaagggtgct
     9361 tgctaaatta cggttttttc cgcttagttt tccaccctgc acattttcat aatttttaca
     9421 gcgacaatcc gcaccgccat ttctgtattt ttcagacaaa acgggaatat ccaacagacc
     9481 acacatatca ttgattgcca agtcaaaacg tcggataaca ccgtcacagt caagacagcg
     9541 gtttaaaaat gaataccaat ctctgttctg tgcctgcaag acatattcca tgttgcgtga
     9601 gcctgcccct tttaattcca agaatacgcc catgtgttcg tcatcagaag ccatgacctt
     9661 gatttctccg taggcgtaat gctctttata gccataatac ccatagtcat aatggataaa
     9721 ataatctgct ttcatgccaa gcacctttcg gataatttcc aatgcgtcag tcgttggata
     9781 acggacagac agataatcaa cgagcagata aaaaggctta tcgcagatat aataattcag
     9841 acaacgcagg ataatatttt gtgtttcctt gctggcgttg gcttttccgt tctcaaagcg
     9901 gttgatacta tatcttgata catgggtaag acttgcaagt tctccttgtg aaagaccgct
     9961 tgcaagacgt ttgttcttca tttcaagtgc aaattctaaa ttgttcattg tattcctcca
    10021 atcccacaat agccttgaaa tacctgtaaa cctctattca aaaagtgtga tatgcactat
    10081 cttcttaaaa cttctcatta ctactcacaa ctttgggatt gtatggtttt tgacttgtct
    10141 tttgtgaaat agtgcccccc tgttagataa cgggggcaca gacaaggctc gcaggctcgc
    10201 cagccgacaa agcatttctt ccccttgaca caaggtgtca tggcggtaag cctgagaggc
    10261 ttccgccacc gtgtcagaaa tactttgtca gcaagttctg ttttgccttt tctaacaagt
    10321 tccatttcaa ccgatattca tatctacgga tttaatcaga taggtatttc tttttgaatg
    10381 tttttatatt tcattctatt gtgcttattc gattacaaga acatagtaat tcttatatgc
    10441 ttatttgtca atatcttttt ttgcctttac aaatcatatt aagcatgata gacttataat
    10501 agcgaagata taacgagata tggagggtta tgcaatgaaa ataagccatt taggaaataa
    10561 tatacagacc ataagaaaat ttaggggaat gaaacaacag gaacttgcgg acaaaatcgg
    10621 tatcaatatg cagagccttt ccaagattga aagagggttg aactatcctg cctatgaaac
    10681 gctggaaaag ataatggaag tgctggacgt tacgcccaat gaattgttgt cgggagaatg
    10741 gaagtatgtc aatcaatctg aaaaggaagt ctgtcagttt ttaagagccg aagaacgtct
    10801 aaatgcagaa ctgaaacatg gacactatga taacttcttt gacagcgagg aagaatggct
    10861 ggaatatgag ttagaaaagt tacgggaata tattacagac tatatcaacg ggaaaagcat
    10921 tgaagcgtcc gacctttacc caatcaaaga atttatccag catttgaagt ttcaaaaatt
    10981 attagaccgc tatgatgatt tatacagtat ggatatgttt ggggaaagca tagagggaca
    11041 taagtataag acaccttatc aagtcgtgaa aatgataaac ccgaactcaa aagaagatat
    11101 ggaactgtta cgagaagtat taaggaataa caattttgat gatgaggacg agtaactttt
    11161 ttcctcttgc gagaagatac agcaaacaaa aagcccgatc aagagtgcaa agcaccacca
    11221 atggtggcaa agctcttgac agggcttttt cttttctgtt gtgggcaatc aagaggaaga
    11281 aagggataaa atctgatttg tggtaaatca ggcaaaggca acgcaggtat ctcaatttga
    11341 tttggtactt gaaaagaacg ggagtaattg gaagattata gaataacaaa tattggtaca
    11401 ttattacagc tattttgtaa tcacgtactc tctttgataa aaaattggag attcctttac
    11461 aaatatgctc ttacgtgcta ttatttaagt atctatttaa aaggagttaa taaatatgcg
    11521 gcaaggtatt cttaaataaa ctgtcaattt gatagtggga acaaataatt ggatgtcctt
    11581 ttttaggagg gcttagtttt ttgtacccag tttaagaata cctttatcat gtgattctaa
    11641 agtatccgga gaatatctgt atgctttgta tgcctatggt tatgcataaa aatcccagtg
    11701 ataaaagtat ttatcactgg gatttttatg cccttttggg tttttgaatg gaggaaaatc
    11761 acatgaaaat tattaatatt ggagttttag ctcatgttga tgcaggaaaa actaccttaa
    11821 cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta
    11881 caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa
    11941 taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg
    12001 atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt
    12061 ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg
    12121 ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg
    12181 tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac
    12241 tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag
    12301 agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac
    12361 tcgaacaaga ggaaagcata agatttcaga attgttctct gttccctctt tatcatggaa
    12421 gtgcaaaaag taatataggg attgataacc ttatagaagt tattactaat aaattttatt
    12481 catcaacaca tcgaggtccg tctgaacttt gcggaaatgt tttcaaaatt gaatatacaa
    12541 aaaaaagaca acgtcttgca tatatacgcc tttatagtgg agtactacat ttacgagatt
    12601 cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact tcaataaatg
    12661 gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg caaaatgagt
    12721 ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga aaaaagattg
    12781 aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa cagagagaaa
    12841 tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga tattacgtgg
    12901 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
    12961 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
    13021 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
    13081 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
    13141 atgagagctc ggtttctctt ggatacttaa atcaatcgtt tcaaaatgca gttatggagg
    13201 ggatacgcta tggctgtgaa caaggattgt atggttggaa tgtgacggac tgtaaaatct
    13261 gttttaagta tggcttatac tatagccctg ttagtacccc agcagatttt cggatgcttg
    13321 ctcctattgt attggaacaa gtcttaaaaa aagctggaac agaattgtta gagccatatc
    13381 ttagttttaa aatttatgcg ccacaggaat atctttcacg agcatacaac gatgctccta
    13441 aatattgtgc gaacatcgta gacactcaat tgaaaaataa tgaggtcatt cttagtggag
    13501 aaatccctgc tcggtgtatt caagaatatc gtagtgattt aactttcttt acaaatggac
    13561 gtagtgtttg tttaacagag ttaaaagggt accatgttac taccggtgaa cctgtttgcc
    13621 agccccgtcg tccaaatagt cggatagata aagtacgata tatgttcaat aaaataactt
    13681 agtgtatttt atgttgttat ataaatatgg tttcttgtta aataagatga aatatttttt
    13741 aataaagatt tgaattaaag tgtaaaggag gagatagtta ttataaacta caagtggata
    13801 ttgtgtcctg tatgtggaaa taaaacacga ttaaagataa gggaagatac tgaattaaaa
    13861 aaattccccc tctattgtcc gaaatgcaga caagaaaatt taattgaaat aaagcagttc
    13921 aaagtaactg tgattacaga gccagacgca aagacgcaga gccgataaaa tgagattaat
    13981 acaatctcat tttatcggct ctttccgtta tgtatggatt cttttaatta gtcttcgatg
    14041 tttcttgctt cgttgatacc gctggctaaa gattccatta aggatagttc tttgtctgta
    14101 aagctatcca tgtatttctc tatctgtaat cgtcgggtgc tttttaccaa gttattagca
    14161 ggtaagaaaa attcatcaac ggaaacatga agtaacgata caaggtcata aagaacttgt
    14221 atgctggggt gttgcccttt attttcaata ttagttaagt accgtgggtc aatttcaatc
    14281 aatgctccca cttgttcacg agttaaacct cgtttcaatc gagcttcttt aatggctaaa
    14341 ccaaaggctc taaaatcata tttatcttct tttttacgca tagtagacca cctctataca
    14401 ttttattgtt cctactgaat taaaaacagg tatagaaaaa cgtgttatat ggtttatagg
    14461 tttatattta ataaaaagca ctactaaacg ccaataaaaa aaaccgttat atggtagtgc
    14521 tatttacgct gttaaaatat tgtatattac ttccaaatgg cggtttgttg gaggtcaacg
    14581 tcgccatgaa gtacatcata tacaataaat ttccttacat tgggttcttg tcaaaaaaag
    14641 tcgtctatct gcaatagata agtacgtcca ccaatgtggt tttataaatc atatagatag
    14701 aataacagaa gcatgtaaac agagaaataa atctgtttat atgctttttt ggctattcag
    14761 aactttttta caaagtttat ttatcagtaa tgcaacaaat ccccctttca cattgggact
    14821 aagagtgaaa ggagataaac gagcaaggct cacttccttt cctagacaga aagggggtga
    14881 gattatgagg acatcttctt tcgagcatat cgtcagatta cagttcaatt ctttaatgct
    14941 gattgtcatt aagaacaaag tgaaaagtcg aaacagacag cttgcaagac attctaaacg
    15001 tgaagtattg ttttgtgaaa tagccgaaac gaaacaagtt gaacgtggaa cgattgatac
    15061 ctgtttttat gattgtgtat catttaaagt cttgcacttt acaatttatg tatctgatga
    15121 aacacttggt acagttttac atagactatc agaaaaacag cgtagtgcga ttttattacg
    15181 ttattttcaa ggaatgaacg acagagaaat ttcagaactg taccatgtat cacgttctgc
    15241 tatttgcagt agaagaacta gaggactaag
//
LOCUS       DAZKMK010000277        13213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.guided.077, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000277 DAZKMK010000000
VERSION     DAZKMK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.guided.077"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG8V1_003026"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG8V1_003026"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0331014.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACG8V1_003027"
     CDS             complement(2159..3031)
                     /locus_tag="ACG8V1_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0331015.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACG8V1_003028"
     CDS             complement(3373..4245)
                     /locus_tag="ACG8V1_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0331016.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACG8V1_003029"
     CDS             4373..4942
                     /locus_tag="ACG8V1_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0331017.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACG8V1_003030"
     CDS             5009..5746
                     /locus_tag="ACG8V1_003030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0331018.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACG8V1_003031"
     CDS             5977..7245
                     /locus_tag="ACG8V1_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0331019.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACG8V1_003032"
     CDS             7732..8196
                     /locus_tag="ACG8V1_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0331020.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACG8V1_003033"
     CDS             8180..8809
                     /locus_tag="ACG8V1_003033"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0331021.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG8V1_003034"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG8V1_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0331022.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACG8V1_003035"
     CDS             10688..11134
                     /locus_tag="ACG8V1_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0331023.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACG8V1_003036"
     CDS             complement(11333..11848)
                     /locus_tag="ACG8V1_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0331024.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACG8V1_003037"
     CDS             11965..13167
                     /locus_tag="ACG8V1_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0331025.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAZKMK010000278         8062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.guided.114, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000278 DAZKMK010000000
VERSION     DAZKMK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.guided.114"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG8V1_003038"
     CDS             128..1564
                     /locus_tag="ACG8V1_003038"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0331026.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG8V1_003039"
     CDS             1753..4200
                     /locus_tag="ACG8V1_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0331027.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8V1_003040"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8V1_003040"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0331028.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG8V1_003041"
     CDS             5487..5693
                     /locus_tag="ACG8V1_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0331029.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG8V1_003042"
     CDS             complement(5690..6793)
                     /locus_tag="ACG8V1_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0331030.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8V1_003043"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8V1_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0331031.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DAZKMK010000279         4804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.guided.140, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000279 DAZKMK010000000
VERSION     DAZKMK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.guided.140"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..734
                     /locus_tag="ACG8V1_003044"
     CDS             <1..734
                     /locus_tag="ACG8V1_003044"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0331032.1"
                     /translation="IVTLGGSGCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRR
                     NVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFW
                     EVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRID
                     QQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAM
                     EHRQTLEKTKILGQTLSRFFTILPK"
     gene            992..2494
                     /gene="eat(A)"
                     /locus_tag="ACG8V1_003045"
     CDS             992..2494
                     /gene="eat(A)"
                     /locus_tag="ACG8V1_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0331033.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2561..3088
                     /locus_tag="ACG8V1_003046"
     CDS             2561..3088
                     /locus_tag="ACG8V1_003046"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0331034.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3299..4360
                     /locus_tag="ACG8V1_003047"
     CDS             3299..4360
                     /locus_tag="ACG8V1_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0331035.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4363..>4804
                     /locus_tag="ACG8V1_003048"
     CDS             4363..>4804
                     /locus_tag="ACG8V1_003048"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0331036.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRT"
BASE COUNT         1650 a          814 c         1076 g         1264 t
ORIGIN      
        1 ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct
       61 tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc
      121 attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta
      181 ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
      241 tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag
      301 atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg
      361 gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca
      421 ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg
      481 atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc
      541 gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt
      601 ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac
      661 acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca
      721 tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga
      781 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
      841 gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga
      901 tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata
      961 ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac
     1021 attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac
     1081 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
     1141 tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt
     1201 cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga
     1261 ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt
     1321 atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt
     1381 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     1441 tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag
     1501 ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca
     1561 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     1621 atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc
     1681 agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg
     1741 attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg
     1801 gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga
     1861 aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca
     1921 ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga
     1981 gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc
     2041 aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga
     2101 aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg
     2161 aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa
     2221 cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat
     2281 gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac
     2341 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     2401 cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt
     2461 atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg
     2521 tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca
     2581 cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac
     2641 tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc
     2701 aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc
     2761 tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga
     2821 ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt
     2881 acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa
     2941 agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag
     3001 agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga
     3061 taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt
     3121 caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata
     3181 taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt
     3241 ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga gggataatat
     3301 gtttggcttt tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg
     3361 ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga
     3421 tgtttcggca tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact
     3481 agaattgatg gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga
     3541 tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa
     3601 tcagcagatt gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac
     3661 gcctaaagat atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg
     3721 gcataattat tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa
     3781 tcaatggctc aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt
     3841 gagaggccca ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct
     3901 tgcaggcgct ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg
     3961 tctgtcagaa aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct
     4021 ccatacggaa cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg
     4081 gcaagacgat gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc
     4141 tatcccagca agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa
     4201 gtatttgcgc tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa
     4261 agtgaatgta gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc
     4321 tggtatgaac tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta
     4381 gagaacctaa caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt
     4441 tctcaagccg ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa
     4501 gaactgacaa gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga
     4561 cgtctgattt atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa
     4621 tgcgtaccga cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa
     4681 gaagcaatga ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat
     4741 ctaatcaagc tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt
     4801 acac
//
LOCUS       DAZKMK010000280         4802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.guided.141, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000280 DAZKMK010000000
VERSION     DAZKMK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.guided.141"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8V1_003049"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8V1_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0331037.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG8V1_003050"
     CDS             763..1917
                     /locus_tag="ACG8V1_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0331038.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACG8V1_003051"
     CDS             2132..3100
                     /locus_tag="ACG8V1_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0331039.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8V1_003052"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8V1_003052"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0331040.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACG8V1_003053"
     CDS             4130..4738
                     /locus_tag="ACG8V1_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0331041.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DAZKMK010000281         4286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.guided.152, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000281 DAZKMK010000000
VERSION     DAZKMK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.guided.152"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..650
                     /gene="dfrG"
                     /locus_tag="ACG8V1_003054"
     CDS             153..650
                     /gene="dfrG"
                     /locus_tag="ACG8V1_003054"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0331042.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(722..2674)
                     /locus_tag="ACG8V1_003055"
     CDS             complement(722..2674)
                     /locus_tag="ACG8V1_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0331043.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2681..2917)
                     /locus_tag="ACG8V1_003056"
     CDS             complement(2681..2917)
                     /locus_tag="ACG8V1_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0331044.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3319..3609
                     /locus_tag="ACG8V1_003057"
     CDS             3319..3609
                     /locus_tag="ACG8V1_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0331045.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(3881..4230)
                     /locus_tag="ACG8V1_003058"
                     /pseudo
     CDS             complement(3881..4230)
                     /locus_tag="ACG8V1_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1348 a          720 c          751 g         1467 t
ORIGIN      
        1 aattgaatga aaatttgtga ttttgtggta ttatgataat ataacttata gaaaaaattg
       61 cttattcaac taacgggcag gataatgaga aaaggttttt tgatagaaat aagaaattag
      121 atggtaaatt agttaaaaag gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg
      181 ataagaatag agtgattggc aaagagaatg acattccttg gaggattccc aaggactggg
      241 aatatgttaa aaatactaca aagggacatc cgataatatt aggtaggaag aaccttgaat
      301 caatcggaag agccttacct gacagaagaa atattattct gacgagagat aaggggttta
      361 cctttaatgg ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg
      421 aagaagaaat ttttattttc ggaggagaac agatttataa tttgtttttc ccttatgttg
      481 agaaaatgta catcacaaaa atacatcatg aattcgaagg agatactttt tttccagaag
      541 tgaattatga ggaatggaat gaggtatttg cccaaaaagg gataaagaat gataaaaatc
      601 cgtataacta ctattttcat gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt
      661 aattgaagaa gaacagtaaa gataaagcac aagagcttgg gattactcca agctcttttt
      721 gtcatttgaa ataaattata tctttccaat caaattgttt atctgtaatc cctaatcttt
      781 gagcaggtgt caaaaccttt ttatccgctg atttaaaagg gcgacaaaag ttgtagtaag
      841 ttcttagaat tgtaatcgca aattgagcat acttcggatt gaagttagcg taaatatagc
      901 tcttcttatc accacgagct gttgttaatg gtctttcaag agatgatata taacgtctta
      961 tctgttgaat aaagctattg gtcgaatggt cattaacatt taatagcatc tttgcgattt
     1021 cgtttggttc cagagcagat aaatccgttc tacaatcaac tgaataaaat cctttatcct
     1081 tagtggctaa tggatgtttg atagggttat cggcatattt gcgatatctt tctccatctt
     1141 tactaatagc ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga taatgagatg
     1201 ccaaagtccg tagaaacttt gttttgaatc ctctgatatc tccccaatcg agcagttctg
     1261 cttttgcttg tccgaattct tttaagcatt gttttctgct tttcgtcttg ttaacttggc
     1321 aaataaaatg atgtgcatcg ctcaacctta attctttcgt gaatactcta taaaaggcgt
     1381 ttctaataga gaagtcatca tcactaatca ttcgccattc gtcggcatta accatttgtt
     1441 taattaacca atagtgagca gttgtagtat aaggagcatc aacatttaaa ccatcgacaa
     1501 atctactcct attagtaatt ttccctaatt cgtgaagata agcattccta ttttcgctat
     1561 cgttagcgga tggttcttgt ggataataag accaatctaa cctatcattt ttacgactaa
     1621 aagtattgag atggtcttct ttgaattttc ttgtatcttc atttaattca tccattgaaa
     1681 tattccaatc ataggcaaca tcagaacgga acacatatct tgataagacc tcagcagata
     1741 caactatata cgtttgcata ttaaggtctt caaaccccgt atatttcttt gaaccttgtc
     1801 ctttcttacg gacgttattt aggtagtagt gcatcttgtc cgtattaagc cacatctcgt
     1861 taaatttctt tgtttgaagg ggttgtgtct catatcgttc taaaaactcc aaacaacgcc
     1921 tatacagcca ctccagctta tgatagtagg ttccaactcc aattcctaaa atatcacaag
     1981 tacgactgac tgaaaccctt ccaacaacca tcttagcgaa cattggaaga atggtgttct
     2041 tttgttggtg ataagtagtg gtttcttctc tttttggtag aacattagtg aacttcttac
     2101 acgccttaca ttgatagcgt tgagatttgc ccctactttt cccccttttg taaaattgct
     2161 tcggctcatt gaatggggtt gattcttctt ccgagcagcc ttctttatga aattgataat
     2221 ctggctcaac atcttggatg ctattgattt ctataaggcg tttaatttct tcaacaacag
     2281 accaattaga taaaggggtt actgtacagt tttgcgatac acctctgcca ataggattgt
     2341 cattgcagta cataccttta tgacctttat ctttggaact accagtcatt ttatatcgac
     2401 ttggtttccc ttttacatct ttgaatttgt gctgttcttt cccaaaatta caacaaaacg
     2461 gattgatgca gtaattatat tgaatgggat attcagaacc gttccattta aaagacgttg
     2521 gcaagaacaa cattttgtta taccttgttg caaatcgttt tggagttaat aaatcatatt
     2581 gtttagccct atcttttaat tcttcgtttg aaatcggaat tttaacatct acaatttcat
     2641 cttccttcgt ggctaatctt ttaagcttgg tcattgtcta tcaacccact ctatctttga
     2701 tacccatttt ttcaacaatc cgcttattac ttcgcttgtt ggcaatccat tcaataaact
     2761 tcttatcgtc taaaatgttc aataagaatt catccactgc ttcgctttca gtacgctctg
     2821 catattcagc gtattgctta actatcaccc ttacttgctc tgatatttcc caatccacag
     2881 acttagcgtt cttatttttt ggctcaagaa attgcatagg aaaatgcacc cctttttgct
     2941 tagtaaattg taaccatttt aacacaccaa tttgcattcc tacaataatt atttcacgtt
     3001 cgcattttta tatacatttt aacttacatt ttgtggtgta aattaacaac aaacgttgat
     3061 ttaacaataa aaaacgagta tcaaattcag ttgcaatttg atactcattt aggtttacat
     3121 tttcactttt tgtactccaa aactgaacta cttaaatcta tactgctgcc tctttttttt
     3181 agtacatgtc cttcatcttc gcaggaagtg ccaatatgat tttaagaaag gtaatagaca
     3241 aaaagtcaaa aagataaaac agagtttctt ctttacaaag ggaatgtatg ttcgtataat
     3301 ggtattgagg tgaaggttat ggagaaaaaa tatggaacag tagataagat ccgtactttg
     3361 caagttagtg ccagaccgtt gatccgtttc agtttaaatg gggtcaattg tttgattgct
     3421 tcccatagtt tgaacttcct agctgaagta gacgaaggga tgaagttagt cgttacaggt
     3481 tattataata atagaaagca atttgttgtg agatcctatc atgtgttagg gaaacccaaa
     3541 attgtggtag aatatgagaa aagtctatac ccaagaaaaa aggtccgaca agatgaaaga
     3601 ataaactaga aaacaagcgg ctgagacaaa aatgtcctag ccgcttgttt tggaaagatg
     3661 gatgcagacg atgggatttg aacccacacg agcatacgct cacaagaacc tcaatctata
     3721 tataactaca tgtatttaag tatttgtgat gaatcactaa actttccaaa ctcatttgag
     3781 ggatattgct taaagattga acagatcaat tcgaagccct attagtattc gctcaatggg
     3841 gggatcagag gccataaaaa gagactagtc attactagtc tcaggtagtg gaattgtagg
     3901 attttaaacg ttcctggaga gtaccggtat gtaactcgaa gaggtgatta tcatagtcgt
     3961 aaaagtaaat tgaagaagcc tcgcctttca cacgagaacg tcctggttcg acagttaaac
     4021 ctaacatttg gatttttgag acaaacgaat ctatatcttg ttcgtcaatt ttaaaagcaa
     4081 tatgattgta tgtctttggc agtttattcg acgaattctg cattactgca atccataggt
     4141 ccttaactaa gaaaaatttt tctggatata atgaaaattt ttttgttgac tatcgtagat
     4201 ttcttttgca tcaaataagg tatttaacaa ttaagtattt aaaagataac ttttaagtta
     4261 tctttaagta atgcttaaag ctatgt
//
LOCUS       DAZKMK010000282         3153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.guided.172, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000282 DAZKMK010000000
VERSION     DAZKMK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.guided.172"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG8V1_003059"
     CDS             260..1171
                     /locus_tag="ACG8V1_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0331046.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8V1_003060"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8V1_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0331047.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="ACG8V1_003061"
     CDS             2328..2612
                     /locus_tag="ACG8V1_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0331048.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT         1169 a          446 c          582 g          956 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa gtt
//
LOCUS       DAZKMK010000283         1691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.guided.199, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000283 DAZKMK010000000
VERSION     DAZKMK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.guided.199"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..200
                     /locus_tag="ACG8V1_003062"
     CDS             <1..200
                     /locus_tag="ACG8V1_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0331049.1"
                     /translation="AMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDI
                     PGMADEMRRPMMLIVSAKKKM"
     gene            233..1141
                     /locus_tag="ACG8V1_003063"
     CDS             233..1141
                     /locus_tag="ACG8V1_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0331050.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1138..1618
                     /locus_tag="ACG8V1_003064"
                     /pseudo
     CDS             1138..1618
                     /locus_tag="ACG8V1_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          598 a          244 c          372 g          477 t
ORIGIN      
        1 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
       61 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
      121 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
      181 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
      241 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gtattcgaat
      301 tgtgaccctt gaggggtcac gcgcaaatat taatatacct aaagatgaat ttcaggatta
      361 tgatattaca tattttgtaa gtgatataga accgtttata tctaatgatg actggcttaa
      421 tcaatttggg aatataataa tgatgcaaaa gccggaggat atggaattat tcccacctga
      481 agaaaaggga ttttcctatc ttatgctatt tgatgattac aataaaattg atcttacctt
      541 attgcccttg gaagagttag ataattacct aaagggcgat aaattaataa aggttctaat
      601 tgataaagat tgtagaatta aaagggacat agttccgact gatatagatt atcatgtaag
      661 aaagccaagc gcaagggagt atgatgattg ctgcaatgaa ttttggaatg taacacctta
      721 tgttattaaa ggattgtgcc gtaaggaaat tttatttgct attgatcatt ttaatcagat
      781 tgttcgccat gagctgctga gaatgatatc atggaaggtc ggcatcgaaa caggctttaa
      841 attaagtgta ggcaagaact ataagtttat tgaaaggtat atatccgagg atttgtggga
      901 gaaacttttg tccacctacc ggatggattc ctatgaaaac atatgggaag cattatttct
      961 atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc attatgccta
     1021 tccggagtat gataggaata taacaaaata taccagggac atgtataaaa aatacactgg
     1081 taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg
     1141 attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag
     1201 gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc
     1261 tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca
     1321 gattgccttg aatatattga caatactgat aagataatat atcttttata tagaagatat
     1381 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
     1441 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
     1501 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
     1561 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
     1621 tgcaaggaac agtgaattgg agttcgtctt gtggttctgt tgcaaagttt taaataaaga
     1681 ataaaatccc t
//
LOCUS       DAZKMK010000284         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.guided.224, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000284 DAZKMK010000000
VERSION     DAZKMK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.guided.224"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACG8V1_003065"
     CDS             26..76
                     /locus_tag="ACG8V1_003065"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0331051.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8V1_003066"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8V1_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0331052.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
LOCUS       DAZKMK010000285         1071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3665
            SAMEA115706854-rid22575873.guided.228, whole genome shotgun
            sequence.
ACCESSION   DAZKMK010000285 DAZKMK010000000
VERSION     DAZKMK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706854
            Sequence Read Archive: ERR13196588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287799.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 37 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706854-rid22575873.guided.228"
                     /isolate="VRE3665"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..550
                     /gene="catA"
                     /locus_tag="ACG8V1_003067"
     CDS             <1..550
                     /gene="catA"
                     /locus_tag="ACG8V1_003067"
                     /EC_number="2.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0331053.1"
                     /translation="DITLFKDMIKKKGYEIYPSLIYAIMEVVNKNKVFRTGINSENKL
                     GYWDKLNPLYTVFNKQTEKFTNIWTESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIP
                     ENTIPISMIPWIDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSVCD
                     GYHASLFMNEFQDIIHRVDDWI"
BASE COUNT          379 a          128 c          171 g          393 t
ORIGIN      
        1 tgatattact ttgtttaaag atatgataaa aaagaaagga tatgaaattt atccctcttt
       61 aatttatgca attatggaag ttgtaaataa aaataaagtg tttagaacag gaattaatag
      121 tgagaataaa ttaggttatt gggataagtt aaatcctttg tatacagttt ttaataagca
      181 aactgaaaaa tttactaaca tttggactga atctgataaa aacttcattt ctttttataa
      241 taattataaa aatgacttgc ttgaatataa agataaagaa gaaatgtttc ctaaaaaacc
      301 gatacctgaa aacaccatac cgatttcaat gattccttgg attgatttta gttcatttaa
      361 tttaaatatt ggtaacaata gcagcttttt attgcctatt attacgatag gtaaatttta
      421 tagtgagaat aataaaattt atataccagt tgctctgcaa cttcatcatt ctgtatgtga
      481 tggttaccat gcttcactat ttatgaatga atttcaagat ataattcata gggtagatga
      541 ttggatttag tttttagatt ttgaaagtga atttaatttt atacacgtaa gtgatcataa
      601 aatttatgaa cgtataacaa ccacattttt tggttgcttg tggttttgat tttgaatttg
      661 gttttgaact tatggactga tttattcagt ccattttttg tgcttgcaca aaaactagcc
      721 tcgcagagca cacgcattaa tgacttatga aacgtagtaa ataagtctag tgtgttatac
      781 tttacttgga agatgcaccg aataaaaaat attgaagaac aactagcaaa agattttaaa
      841 gagttatttt attttaagtc tttataacat gagtgaagcg aatttttaaa tttcgataga
      901 aatttttaca tcaaaaagcc ccctgtcaaa attgacgaag ggggtttttt ggcgcacgct
      961 tttcgttaga aatatacaag attgaaaatc gtgtataagt gcgccctttg ttttgaactt
     1021 agcacgttac atcaattttt taaaatgatg tataagtgcg cccttttaaa t
//
